Prupe.1G415900_v2.0.a1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0017074
Physical Location & Seq
Forward (+)
36126542 .. 36128760
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G415900.1

Sequence Viewer

Length: 1245 bp
ATGTTCTCGTTAGCATTGGGATTGTTTGTTTTTTGTGTTAGTGCAGCAGAACATCACCATGTGAAGGCTAAAACTAAACGAATTTCTTTGGAAGTTCATAAGAAGTTGAAGCTTTTGAATAAGCGTGGCGTGAAGACTATCAAGAGTGAGGATGGAGATATCATTGACTGTGTTGACATCTACAAACAGCCTGCTTTTGATCATCCGGCATTGCGCAACCACAAAATTCAGATGAGACCCAGTAATCATGTACAAAATGATCATAATTCTTCTCAGCTGGTATCTCAAATTTGGCAAAGGAGCGGAAGCTGTCCAGACGGAACCATTCCCATTCGGAGAATTCGAAAGCAAGATTTATTGAGAGCTACTTCACTTGAAAACTTTGGGAAGAAAATGCCAGTGCATTCTTCCTACTCCCCCACAGATGACAAGGGCACTGTGTACATCAACAGCACCCGAGTCGAACTCGGGCCTCAAGTCAATCGTTCAGCTGCTATCCTGTTAACTACTGGATACAATTACATAGGTGCTCAAGGAGATATAAATATTTGGACTCCAAGAGTTGAATCACCAGATGAGTTCACTACTGCTCAAATCTGGCTTAAGAATGGACCTGGAGAGGCTTTTGAAAGTGTAGAATCAGGCTGGGTGGTAAACCCAAAGGTATATGGTAATGGAGCAACTCGACTTTTTGTATATTGGACTAAGGACTCATACAAATCATCAGGCTGCTTTGATCTCACCTGCCCAGGATTTGTGCAAACCAACAAAGACATAGCCCTTGGTATGGTCCTTGGACCTGTATCATCTAAAATGGGACCCCAATATCAAACCACCTTCAGCATCACCAAGGAACCAAGCACAGGAAATTGGTGGGTGCGTATTGGCCAAAACGTTCCTGTGGGATATTTTCCTGGAGAGCTCTTCTATTATTTGACACGCGGTGCTGCAACATTAGTTGAATGGGGAGGAGAAGTGTTTAGCTCCAAGGTGAAGCAAAATCATCCACACACTGCAACAGGGATGGGAAGTGGAGATTTTGCATCGGGCAAATTGGGCAATGCATGCTATGTGAAGCAAGTTAGGATTATAGATTATTCAAAGCAATTGAAGTACCCAGAATGGGTAGGCACTTATTCAGATGAAGAGTATTGCTACTCTGCATTGAACTACGCTCTGTCACTTGCTCAAGAACCTGTGTTCTACTTCGGCGGACCGGGTCGAAATCCTCCTTATTGTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

415

Amino Acids

46.18

Weight (kDa)

8.97

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 752
Acc16I TGCGCA 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 752
AccBSI CCGCTC 1 cut(s) 303
AccII CGCG 1 cut(s) 942
AciI CCGC 3 cut(s) 303, 942, 1212
AclI AACGTT 1 cut(s) 894
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 4 cut(s) 81, 225, 288, 339
AcuI CTGAAG 1 cut(s) 823
AdeI CACNNNGTG 2 cut(s) 61, 944
AfaI GTAC 3 cut(s) 252, 443, 1115
AfiI CCNNNNNNNGG 4 cut(s) 64, 787, 863, 1123
AflII CTTAAG 1 cut(s) 602
AgsI TTSAA 9 cut(s) 109, 118, 377, 566, 629, 962, 1101, 1111, 1168
AjnI CCWGG 3 cut(s) 613, 748, 913
AluBI AGCT 7 cut(s) 112, 277, 309, 365, 491, 922, 984
AluI AGCT 7 cut(s) 112, 277, 309, 365, 491, 922, 984
Alw21I GWGCWC 2 cut(s) 532, 924
Alw26I GTCTC 1 cut(s) 229
Ama87I CYCGRG 2 cut(s) 456, 467
AoxI GGCC 2 cut(s) 470, 886
ApeKI GCWGC 4 cut(s) 44, 491, 729, 947
ApoI RAATTY 4 cut(s) 81, 225, 288, 339
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 6 cut(s) 470, 611, 790, 797, 818, 1214
AsuC2I CCSGG 1 cut(s) 1218
AsuHPI GGTGA 5 cut(s) 47, 561, 733, 838, 1003
AsuII TTCGAA 1 cut(s) 343
AvaI CYCGRG 2 cut(s) 456, 467
AvaII GGWCC 5 cut(s) 611, 790, 797, 818, 1214
BaeGI GKGCMC 1 cut(s) 437
BalI TGGCCA 1 cut(s) 888
BanII GRGCYC 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 532, 924
BbvI GCAGC 4 cut(s) 56, 478, 716, 934
BccI CCATC 2 cut(s) 146, 1018
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 3 cut(s) 615, 750, 915
BciVI GTATCC 1 cut(s) 506
BclI TGATCA 2 cut(s) 199, 259
BcnI CCSGG 1 cut(s) 1218
BcoDI GTCTC 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 752
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 4 cut(s) 45, 492, 730, 948
BlsI GCNGC 4 cut(s) 46, 493, 731, 949
Bme1390I CCNGG 4 cut(s) 615, 750, 915, 1218
Bme18I GGWCC 5 cut(s) 611, 790, 797, 818, 1214
BmeT110I CYCGRG 2 cut(s) 456, 467
BmgT120I GGNCC 6 cut(s) 470, 611, 790, 797, 818, 1214
BmiI GGNNCC 4 cut(s) 322, 819, 820, 855
BmrFI CCNGG 4 cut(s) 615, 750, 915, 1218
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 852, 1052
BmuI ACTGGG 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 636, 936
Bpu14I TTCGAA 1 cut(s) 343
BpuEI CTTGAG 3 cut(s) 459, 516, 1173
BpuMI CCSGG 1 cut(s) 1218
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 5 cut(s) 748, 781, 793, 849, 987
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 787, 863, 1123
Bse1I ACTGG 3 cut(s) 240, 398, 514
Bse3DI GCAATG 2 cut(s) 209, 1066
BseBI CCWGG 3 cut(s) 615, 750, 915
BseDI CCNNGG 5 cut(s) 748, 781, 793, 849, 987
BseGI GGATG 4 cut(s) 157, 202, 1003, 1029
BseLI CCNNNNNNNGG 4 cut(s) 64, 787, 863, 1123
BseMI GCAATG 2 cut(s) 209, 1066
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 3 cut(s) 240, 398, 514
BseRI GAGGAG 1 cut(s) 984
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 4 cut(s) 56, 478, 716, 934
BseYI CCCAGC 1 cut(s) 645
BsgI GTGCAG 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 942
BshFI GGCC 2 cut(s) 472, 888
BsiHKAI GWGCWC 2 cut(s) 532, 924
BsiHKCI CYCGRG 2 cut(s) 456, 467
BsiSI CCGG 2 cut(s) 206, 1217
BslFI GGGAC 2 cut(s) 831, 1224
BslI CCNNNNNNNGG 4 cut(s) 64, 787, 863, 1123
BsmAI GTCTC 1 cut(s) 229
BsmFI GGGAC 2 cut(s) 831, 1224
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 2 cut(s) 472, 888
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 456, 467
Bsp119I TTCGAA 1 cut(s) 343
Bsp1286I GDGCHC 3 cut(s) 437, 532, 924
Bsp1407I TGTACA 2 cut(s) 250, 441
Bsp143I GATC 3 cut(s) 199, 259, 736
BspACI CCGC 3 cut(s) 303, 942, 1212
BspANI GGCC 2 cut(s) 472, 888
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 1 cut(s) 942
BspLI GGNNCC 4 cut(s) 322, 819, 820, 855
BspMI ACCTGC 1 cut(s) 752
BspQI GCTCTTC 1 cut(s) 929
BspT104I TTCGAA 1 cut(s) 343
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 303
BsrDI GCAATG 2 cut(s) 209, 1066
BsrGI TGTACA 2 cut(s) 250, 441
BsrI ACTGG 3 cut(s) 240, 398, 514
BssECI CCNNGG 5 cut(s) 748, 781, 793, 849, 987
BssMI GATC 3 cut(s) 199, 259, 736
BssT1I CCWWGG 4 cut(s) 781, 793, 849, 987
Bst2UI CCWGG 3 cut(s) 615, 750, 915
Bst4CI ACNGT 2 cut(s) 170, 439
Bst6I CTCTTC 2 cut(s) 929, 1140
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 1065
BstAUI TGTACA 2 cut(s) 250, 441
BstBI TTCGAA 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 192, 1066
BstDEI CTNAG 2 cut(s) 273, 705
BstF5I GGATG 4 cut(s) 157, 202, 1003, 1029
BstFNI CGCG 1 cut(s) 942
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 3 cut(s) 202, 262, 739
BstMAI GTCTC 1 cut(s) 229
BstMBI GATC 3 cut(s) 199, 259, 736
BstMWI GCNNNNNNNGC 2 cut(s) 1056, 1065
BstNI CCWGG 3 cut(s) 615, 750, 915
BstNSI RCATGY 1 cut(s) 1068
BstSCI CCNGG 4 cut(s) 613, 748, 913, 1216
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 1 cut(s) 942
BstV1I GCAGC 4 cut(s) 56, 478, 716, 934
BstV2I GAAGAC 1 cut(s) 140
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 2 cut(s) 472, 888
BtsCI GGATG 4 cut(s) 157, 202, 1003, 1029
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 3 cut(s) 405, 435, 1011
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 2 cut(s) 192, 1066
CfoI GCGC 1 cut(s) 216
Cfr13I GGNCC 6 cut(s) 470, 611, 790, 797, 818, 1214
CpoI CGGWCCG 1 cut(s) 1214
Csp6I GTAC 3 cut(s) 251, 442, 1114
CspI CGGWCCG 1 cut(s) 1214
CviAII CATG 3 cut(s) 59, 248, 1065
CviQI GTAC 3 cut(s) 251, 442, 1114
DdeI CTNAG 2 cut(s) 273, 705
DpnI GATC 3 cut(s) 201, 261, 738
DpnII GATC 3 cut(s) 199, 259, 736
DraIII CACNNNGTG 2 cut(s) 61, 944
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 2 cut(s) 929, 1140
EarI CTCTTC 2 cut(s) 929, 1140
EciI GGCGGA 1 cut(s) 1227
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 4 cut(s) 781, 793, 849, 987
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 5 cut(s) 611, 790, 797, 818, 1214
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 823
Eco88I CYCGRG 2 cut(s) 456, 467
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 339
EcoRII CCWGG 3 cut(s) 613, 748, 913
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 4 cut(s) 781, 793, 849, 987
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 4 cut(s) 781, 793, 849, 987
FaeI CATG 3 cut(s) 62, 251, 1068
FaqI GGGAC 2 cut(s) 831, 1224
FatI CATG 3 cut(s) 58, 247, 1064
FbaI TGATCA 2 cut(s) 199, 259
Fnu4HI GCNGC 4 cut(s) 45, 492, 730, 948
FokI GGATG 4 cut(s) 164, 189, 990, 1036
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 4 cut(s) 45, 492, 730, 948
FspI TGCGCA 1 cut(s) 215
GlaI GCGC 1 cut(s) 215
GluI GCNGC 4 cut(s) 45, 492, 730, 948
GsaI CCCAGC 1 cut(s) 649
GsuI CTGGAG 2 cut(s) 636, 936
HaeIII GGCC 2 cut(s) 472, 888
HapII CCGG 2 cut(s) 206, 1217
HhaI GCGC 1 cut(s) 216
Hin1II CATG 3 cut(s) 62, 251, 1068
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HincII GTYRAC 2 cut(s) 175, 504
HindII GTYRAC 2 cut(s) 175, 504
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 5 cut(s) 459, 553, 566, 638, 710
HpaI GTTAAC 1 cut(s) 504
HpaII CCGG 2 cut(s) 206, 1217
HphI GGTGA 5 cut(s) 47, 561, 733, 838, 1003
Hpy166II GTNNAC 5 cut(s) 175, 442, 504, 582, 655
Hpy188I TCNGA 3 cut(s) 231, 336, 1141
Hpy188III TCNNGA 3 cut(s) 142, 314, 1190
Hpy8I GTNNAC 5 cut(s) 175, 442, 504, 582, 655
HpyAV CCTTC 2 cut(s) 58, 847
HpyCH4III ACNGT 2 cut(s) 170, 439
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 8 cut(s) 44, 403, 760, 950, 1016, 1043, 1064, 1163
HpyF10VI GCNNNNNNNGC 2 cut(s) 1056, 1065
HpyF3I CTNAG 2 cut(s) 273, 705
HpySE526I ACGT 1 cut(s) 894
Hsp92II CATG 3 cut(s) 62, 251, 1068
HspAI GCGC 1 cut(s) 214
KflI GGGWCCC 1 cut(s) 818
Ksp22I TGATCA 2 cut(s) 199, 259
KspAI GTTAAC 1 cut(s) 504
Kzo9I GATC 3 cut(s) 199, 259, 736
LguI GCTCTTC 1 cut(s) 929
LmnI GCTCC 3 cut(s) 300, 677, 989
Lsp1109I GCAGC 4 cut(s) 56, 478, 716, 934
LweI GCATC 2 cut(s) 852, 1052
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 1 cut(s) 1179
MalI GATC 3 cut(s) 201, 261, 738
MbiI CCGCTC 1 cut(s) 303
MboI GATC 3 cut(s) 199, 259, 736
MboII GAAGA 6 cut(s) 145, 261, 399, 400, 916, 1157
MfeI CAATTG 1 cut(s) 1106
MhlI GDGCHC 3 cut(s) 437, 532, 924
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 9 cut(s) 81, 225, 265, 288, 339, 517, 868, 1052, 1106
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 3 cut(s) 468, 547, 704
MnlI CCTC 5 cut(s) 142, 483, 613, 962, 1239
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 888
MseI TTAA 2 cut(s) 503, 603
MslI CAYNNNNRTG 1 cut(s) 57
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 2 cut(s) 277, 491
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 2 cut(s) 206, 1217
MspR9I CCNGG 4 cut(s) 615, 750, 915, 1218
MunI CAATTG 1 cut(s) 1106
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 3 cut(s) 615, 750, 915
MvnI CGCG 1 cut(s) 942
MwoI GCNNNNNNNGC 2 cut(s) 1056, 1065
NciI CCSGG 1 cut(s) 1218
NdeII GATC 3 cut(s) 199, 259, 736
NlaIII CATG 3 cut(s) 62, 251, 1068
NlaIV GGNNCC 4 cut(s) 322, 819, 820, 855
NmuCI GTSAC 1 cut(s) 1179
NsbI TGCGCA 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 1068
NspV TTCGAA 1 cut(s) 343
PaeI GCATGC 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 752
PciSI GCTCTTC 1 cut(s) 929
PcsI WCGNNNNNNNCGW 1 cut(s) 340
PctI GAATGC 1 cut(s) 403
PfeI GAWTC 2 cut(s) 566, 638
PflFI GACNNNGTC 1 cut(s) 1218
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 4 cut(s) 46, 493, 731, 949
PleI GAGTC 3 cut(s) 467, 547, 704
PpsI GAGTC 3 cut(s) 467, 547, 704
PpuMI RGGWCCY 1 cut(s) 818
Psp124BI GAGCTC 1 cut(s) 924
Psp1406I AACGTT 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 818
Psp6I CCWGG 3 cut(s) 613, 748, 913
PspFI CCCAGC 1 cut(s) 645
PspGI CCWGG 3 cut(s) 613, 748, 913
PspN4I GGNNCC 4 cut(s) 322, 819, 820, 855
PspPI GGNCC 6 cut(s) 470, 611, 790, 797, 818, 1214
PspPPI RGGWCCY 1 cut(s) 818
PsyI GACNNNGTC 1 cut(s) 1218
PvuII CAGCTG 2 cut(s) 277, 491
RsaI GTAC 3 cut(s) 252, 443, 1115
RsaNI GTAC 3 cut(s) 251, 442, 1114
RseI CAYNNNNRTG 1 cut(s) 57
Rsr2I CGGWCCG 1 cut(s) 1214
RsrII CGGWCCG 1 cut(s) 1214
SacI GAGCTC 1 cut(s) 924
SapI GCTCTTC 1 cut(s) 929
SaqAI TTAA 2 cut(s) 503, 603
SatI GCNGC 4 cut(s) 45, 492, 730, 948
Sau3AI GATC 3 cut(s) 199, 259, 736
Sau96I GGNCC 6 cut(s) 470, 611, 790, 797, 818, 1214
SchI GAGTC 3 cut(s) 468, 547, 704
ScrFI CCNGG 4 cut(s) 615, 750, 915, 1218
SduI GDGCHC 3 cut(s) 437, 532, 924
SfaNI GCATC 2 cut(s) 852, 1052
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 5 cut(s) 611, 790, 797, 818, 1214
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 4 cut(s) 474, 531, 602, 1188
SmoI CTYRAG 4 cut(s) 474, 531, 602, 1188
SphI GCATGC 1 cut(s) 1068
Sse9I AATT 9 cut(s) 81, 225, 265, 288, 339, 517, 868, 1052, 1106
SsiI CCGC 3 cut(s) 303, 942, 1212
SspI AATATT 1 cut(s) 547
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 4 cut(s) 613, 748, 913, 1216
StyI CCWWGG 4 cut(s) 781, 793, 849, 987
TaaI ACNGT 2 cut(s) 170, 439
TaiI ACGT 1 cut(s) 897
TaqI TCGA 4 cut(s) 343, 462, 685, 1222
TasI AATT 9 cut(s) 81, 225, 265, 288, 339, 517, 868, 1052, 1106
TatI WGTACW 2 cut(s) 250, 441
TfiI GAWTC 2 cut(s) 566, 638
Tru1I TTAA 2 cut(s) 503, 603
Tru9I TTAA 2 cut(s) 503, 603
TscAI CASTG 3 cut(s) 405, 442, 1018
TseFI GTSAC 1 cut(s) 1179
TseI GCWGC 4 cut(s) 44, 491, 729, 947
Tsp45I GTSAC 1 cut(s) 1179
TspDTI ATGAA 2 cut(s) 86, 1158
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 3 cut(s) 405, 442, 1018
Tth111I GACNNNGTC 1 cut(s) 1218
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 5 cut(s) 611, 790, 797, 818, 1214
XapI RAATTY 4 cut(s) 81, 225, 288, 339
XceI RCATGY 1 cut(s) 1068
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.