Rorug06G0379900

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017074
Physical Location & Seq
Forward (+)
52595319 .. 52596317
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0379900.1

Sequence Viewer

Length: 357 bp
ATGGAGATGGTGTCGAAGAAGCTTGAGAACCCTGCAGGTGCGGCAATTCTCTGGTCCTTGACAGACCCAAAGCCGGTGGGCAAACTCGAGGCGACGAAGGCTAATTGGTGCAAGCCCAAGAAGGGCAGTGTTTTCCCGGTGAAGAGGAGGCTGGTGAAGACCATGGTGTTTGATTCCATTGTGAAATTCATTGCCTCTGTTTTCTCTTCCTCTAAAGTACCCAATCCCACGATCTCTCTTTCTGCACCCAAAATTCCAAAAGCCAAAAAATGCAACCACACTCGGACATACCCACATCCAAGCCCAGACCATGTTGGCAAGCTAGCTCACAATGAGTCGTCGGTAACGAGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

118

Amino Acids

12.89

Weight (kDa)

10.11

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 26
AciI CCGC 1 cut(s) 41
AcsI RAATTY 2 cut(s) 185, 252
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 73, 122
AluBI AGCT 3 cut(s) 22, 322, 326
AluI AGCT 3 cut(s) 22, 322, 326
Ama87I CYCGRG 1 cut(s) 86
ApoI RAATTY 2 cut(s) 185, 252
AspS9I GGNCC 1 cut(s) 54
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 2 cut(s) 151, 166
AsuNHI GCTAGC 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 164
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 137
BmtI GCTAGC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 122
Bse118I RCCGGY 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 2 cut(s) 73, 122
BseMI GCAATG 1 cut(s) 189
BseRI GAGGAG 1 cut(s) 160
BsgI GTGCAG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 74, 137
BslI CCNNNNNNNGG 2 cut(s) 73, 122
BsoBI CYCGRG 1 cut(s) 86
Bsp143I GATC 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 1 cut(s) 41
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 26
BspOI GCTAGC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 73
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 162
Bst6I CTCTTC 2 cut(s) 137, 211
BstC8I GCNNGC 3 cut(s) 113, 320, 324
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 295
BstKTI GATC 1 cut(s) 234
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 41, 98
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 3 cut(s) 113, 320, 324
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 218
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 2 cut(s) 163, 311
CviJI RGCY 9 cut(s) 22, 73, 101, 115, 151, 263, 303, 322, 326
CviKI_1 RGCY 9 cut(s) 22, 73, 101, 115, 151, 263, 303, 322, 326
CviQI GTAC 1 cut(s) 218
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 137, 211
EarI CTCTTC 2 cut(s) 137, 211
Eco130I CCWWGG 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 166, 314
FaiI YATR 3 cut(s) 164, 289, 312
FatI CATG 2 cut(s) 162, 310
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 1 cut(s) 323
GluI GCNGC 1 cut(s) 42
HapII CCGG 2 cut(s) 74, 137
Hin1II CATG 2 cut(s) 166, 314
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 3 cut(s) 173, 335, 349
HpaII CCGG 2 cut(s) 74, 137
HphI GGTGA 2 cut(s) 151, 166
Hpy188I TCNGA 2 cut(s) 285, 356
Hpy99I CGWCG 2 cut(s) 97, 343
HpyAV CCTTC 2 cut(s) 91, 115
HpyCH4V TGCA 4 cut(s) 35, 111, 245, 273
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 98
Hsp92II CATG 2 cut(s) 166, 314
Kzo9I GATC 1 cut(s) 231
LpnPI CCDG 7 cut(s) 21, 37, 45, 87, 137, 150, 318
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 4 cut(s) 28, 154, 169, 198
MluCI AATT 4 cut(s) 45, 103, 185, 252
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 5 cut(s) 82, 138, 141, 205, 220
MspI CCGG 2 cut(s) 74, 137
MspR9I CCNGG 1 cut(s) 137
MwoI GCNNNNNNNGC 2 cut(s) 41, 98
NciI CCSGG 1 cut(s) 137
NcoI CCATGG 1 cut(s) 162
NdeII GATC 1 cut(s) 231
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 2 cut(s) 166, 314
PaeR7I CTCGAG 1 cut(s) 86
PaqCI CACCTGC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 43
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PspPI GGNCC 1 cut(s) 54
PspXI VCTCGAGB 1 cut(s) 86
PstI CTGCAG 1 cut(s) 37
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 54
SbfI CCTGCAGG 1 cut(s) 37
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 137
SdaI CCTGCAGG 1 cut(s) 37
SetI ASST 4 cut(s) 24, 40, 324, 328
SfcI CTRYAG 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 23, 86
SmoI CTYRAG 2 cut(s) 23, 86
Sse8387I CCTGCAGG 1 cut(s) 37
Sse9I AATT 4 cut(s) 45, 103, 185, 252
SsiI CCGC 1 cut(s) 41
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 162
TaqI TCGA 2 cut(s) 14, 87
TasI AATT 4 cut(s) 45, 103, 185, 252
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 173
TscAI CASTG 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 178
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 2 cut(s) 185, 252
XhoI CTCGAG 1 cut(s) 86
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.