AT5G28150

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004954
Physical Location & Seq
Forward (+)
10135409 .. 10137105
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28150.1

Sequence Viewer

Length: 870 bp
ATGAAGGATTTTCCTTCTTGCTTTGGTGAAAATGGAGTTCAAGTTGCGGATTCATCGTCTTCTTCGAACTCCGGTAAAAACGCTCAGAATTTGGTTACTTGTATCTACCAATGTCGAATTCGCGGAAGAAATTGTTTGATCACTGTGACATGGACTAAGAATCTGATGGGTCAAAGTGTAACAGTTGGTGTTGATGATTCTTGTAACCAAAGTCTTTGTAAAGTCGAGATTAAGCCGTGGCTGTTTACTAAACGGAAAGGGTCTAAGAGTTTAGAGGCGTATTCGTGTAATATCGATGTTTTTTGGGATCTTTCGTCTGCGAAATTCGGATCAGGTCCTGAAGCTTTGGGAGGATTCTATGTTGGTGTTGTTGTTGACAAAGAAATGGTTTTGCTTCTTGGTGATATGAAGAAAGAAGCTTTTAAGAAGACGAATGCTTCGCCTTCGTCTTTAGGTGCTGTGTTCATTGCTAAGAAAGAGCATGTTTTTGGTAAGAGAGTGTTTGCAACGAAAGCTCAGCTTTTTGCTGATGGGAAATTTCATGATCTGTTGATTGAGTGTGACACGAATGTCACCGATCCTTGTCTCGTTGTTCGTGTGGATGGTAAGACGTTGTTGCAGGTGAAGCGGCTTAAGTGGAAGTTTCGTGGCAACGATACGATAGTTGTTAATAAAATGACTGTGGAAGTTCTATGGGATGTTCATAGTTGGCTCTTTGGGTTGCCTACAACGGGAAACGCTGTGTTCATGTTCAGGACTTGTCAATCTACTGAGAAGAGCTTGTCTTTCTCACAGGACGTGACAACAACAAACTCCAAGTCTCATAGTTTTGGCTTTTCGTTGATTCTGTACGCTTGGAAGAGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

289

Amino Acids

32.06

Weight (kDa)

9.02

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 28 - 287 1.8e-106 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 610
AasI GACNNNNNNGTC 1 cut(s) 569
Acc36I ACCTGC 1 cut(s) 610
AccII CGCG 1 cut(s) 123
AciI CCGC 3 cut(s) 47, 123, 628
AclWI GGATC 3 cut(s) 315, 337, 572
AcsI RAATTY 4 cut(s) 88, 117, 323, 536
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 1 cut(s) 851
AfiI CCNNNNNNNGG 1 cut(s) 731
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 1 cut(s) 41
AjiI CACGTC 1 cut(s) 799
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 5 cut(s) 344, 419, 515, 520, 780
AluI AGCT 5 cut(s) 344, 419, 515, 520, 780
Alw26I GTCTC 2 cut(s) 590, 825
AlwI GGATC 3 cut(s) 315, 337, 572
AlwNI CAGNNNCTG 1 cut(s) 338
ApoI RAATTY 4 cut(s) 88, 117, 323, 536
ArsI GACNNNNNNTTYG 4 cut(s) 421, 453, 803, 835
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 4 cut(s) 38, 413, 565, 634
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 335
BaeI ACNNNNGTAYC 2 cut(s) 648, 681
BbsI GAAGAC 2 cut(s) 51, 434
BccI CCATC 3 cut(s) 160, 524, 596
BceAI ACGGC 1 cut(s) 220
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 590, 825
BfrI CTTAAG 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 610
BisI GCNGC 1 cut(s) 629
BlpI GCTNAGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 630
Bme18I GGWCC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 1 cut(s) 335
BpiI GAAGAC 2 cut(s) 51, 434
Bpu1102I GCTNAGC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse3DI GCAATG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 2 cut(s) 607, 703
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 3 cut(s) 98, 530, 762
Bsh1236I CGCG 1 cut(s) 123
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 731
BsmAI GTCTC 2 cut(s) 590, 825
BsmI GAATGC 1 cut(s) 439
Bsp119I TTCGAA 1 cut(s) 65
Bsp143I GATC 5 cut(s) 138, 307, 329, 544, 577
Bsp1720I GCTNAGC 1 cut(s) 516
BspACI CCGC 3 cut(s) 47, 123, 628
BspCNI CTCAG 3 cut(s) 97, 529, 763
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 1 cut(s) 123
BspHI TCATGA 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 3 cut(s) 315, 337, 572
BspQI GCTCTTC 2 cut(s) 770, 854
BspT104I TTCGAA 1 cut(s) 65
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 5 cut(s) 138, 307, 329, 544, 577
Bst4CI ACNGT 3 cut(s) 145, 184, 682
Bst6I CTCTTC 2 cut(s) 770, 854
BstAFI CTTAAG 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 65
BstDEI CTNAG 6 cut(s) 84, 156, 264, 471, 516, 771
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 607, 703
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 5 cut(s) 141, 310, 332, 547, 580
BstMAI GTCTC 2 cut(s) 590, 825
BstMBI GATC 5 cut(s) 138, 307, 329, 544, 577
BstMWI GCNNNNNNNGC 2 cut(s) 512, 625
BstNSI RCATGY 1 cut(s) 485
BstUI CGCG 1 cut(s) 123
BstV2I GAAGAC 2 cut(s) 51, 434
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 236
BtrI CACGTC 1 cut(s) 799
BtsCI GGATG 2 cut(s) 607, 703
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 610
CaiI CAGNNNCTG 1 cut(s) 338
CciI TCATGA 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 294
Csp6I GTAC 1 cut(s) 850
CviAII CATG 4 cut(s) 150, 482, 542, 748
CviQI GTAC 1 cut(s) 850
DdeI CTNAG 6 cut(s) 84, 156, 264, 471, 516, 771
DpnI GATC 5 cut(s) 140, 309, 331, 546, 579
DpnII GATC 5 cut(s) 138, 307, 329, 544, 577
DrdI GACNNNNNNGTC 1 cut(s) 569
DseDI GACNNNNNNGTC 1 cut(s) 569
Eam1104I CTCTTC 2 cut(s) 770, 854
EarI CTCTTC 2 cut(s) 770, 854
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 117
FaeI CATG 4 cut(s) 153, 485, 545, 751
FaiI YATR 9 cut(s) 151, 360, 407, 483, 543, 694, 705, 749, 825
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FatI CATG 4 cut(s) 149, 481, 541, 747
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 1 cut(s) 629
FokI GGATG 2 cut(s) 614, 710
Fsp4HI GCNGC 1 cut(s) 629
GluI GCNGC 1 cut(s) 629
HapII CCGG 1 cut(s) 72
Hin1II CATG 4 cut(s) 153, 485, 545, 751
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 342, 417
HinfI GANTC 5 cut(s) 50, 160, 197, 354, 844
HpaII CCGG 1 cut(s) 72
HphI GGTGA 4 cut(s) 38, 413, 565, 634
Hpy166II GTNNAC 2 cut(s) 246, 376
Hpy188I TCNGA 3 cut(s) 87, 165, 329
Hpy188III TCNNGA 4 cut(s) 226, 338, 542, 754
Hpy8I GTNNAC 2 cut(s) 246, 376
HpyAV CCTTC 2 cut(s) 24, 453
HpyCH4III ACNGT 3 cut(s) 145, 184, 682
HpyCH4IV ACGT 2 cut(s) 611, 798
HpyCH4V TGCA 2 cut(s) 506, 619
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 625
HpyF3I CTNAG 6 cut(s) 84, 156, 264, 471, 516, 771
HpySE526I ACGT 2 cut(s) 611, 798
Hsp92II CATG 4 cut(s) 153, 485, 545, 751
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 5 cut(s) 138, 307, 329, 544, 577
LguI GCTCTTC 2 cut(s) 770, 854
LpnPI CCDG 6 cut(s) 85, 318, 351, 605, 739, 779
MaeII ACGT 2 cut(s) 611, 798
MaeIII GTNAC 7 cut(s) 94, 145, 178, 203, 560, 571, 799
MalI GATC 5 cut(s) 140, 309, 331, 546, 579
MboI GATC 5 cut(s) 138, 307, 329, 544, 577
MboII GAAGA 6 cut(s) 51, 54, 138, 421, 439, 787
MflI RGATCY 1 cut(s) 307
MluCI AATT 5 cut(s) 88, 117, 130, 323, 536
MnlI CCTC 2 cut(s) 268, 344
MseI TTAA 4 cut(s) 231, 423, 633, 669
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 512, 625
NdeII GATC 5 cut(s) 138, 307, 329, 544, 577
NlaIII CATG 4 cut(s) 153, 485, 545, 751
NmuCI GTSAC 4 cut(s) 145, 560, 571, 799
NspI RCATGY 1 cut(s) 485
NspV TTCGAA 1 cut(s) 65
PagI TCATGA 1 cut(s) 541
PaqCI CACCTGC 1 cut(s) 610
PciSI GCTCTTC 2 cut(s) 770, 854
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 5 cut(s) 50, 160, 197, 354, 844
PkrI GCNGC 1 cut(s) 630
PpuMI RGGWCCY 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 335
PspPI GGNCC 1 cut(s) 335
PspPPI RGGWCCY 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
SapI GCTCTTC 2 cut(s) 770, 854
SaqAI TTAA 4 cut(s) 231, 423, 633, 669
SatI GCNGC 1 cut(s) 629
Sau3AI GATC 5 cut(s) 138, 307, 329, 544, 577
Sau96I GGNCC 1 cut(s) 335
SfuI TTCGAA 1 cut(s) 65
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 5 cut(s) 88, 117, 130, 323, 536
SsiI CCGC 3 cut(s) 47, 123, 628
TaaI ACNGT 3 cut(s) 145, 184, 682
TaiI ACGT 2 cut(s) 614, 801
TaqI TCGA 4 cut(s) 65, 115, 225, 294
TasI AATT 5 cut(s) 88, 117, 130, 323, 536
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 5 cut(s) 50, 160, 197, 354, 844
Tru1I TTAA 4 cut(s) 231, 423, 633, 669
Tru9I TTAA 4 cut(s) 231, 423, 633, 669
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 4 cut(s) 145, 560, 571, 799
Tsp45I GTSAC 4 cut(s) 145, 560, 571, 799
TspDTI ATGAA 7 cut(s) 17, 42, 422, 454, 530, 692, 736
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 1 cut(s) 148
Vha464I CTTAAG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 4 cut(s) 88, 117, 323, 536
XceI RCATGY 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.