pycom12g21830

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004954
Physical Location & Seq
Forward (+)
22956450 .. 22957058
609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21830.1

Sequence Viewer

Length: 609 bp
ATGAGAGATTTCCCATCTTGTTTTGGTGAAAATGGGGTTCAGGTTGCTGATTCTTCATCATCAAATAGTAGTAGGGGTGCCCAGAATTTGGTTACTTGTATGTATCAATGCCGGTTACGAGGCCGGTCTTGCTTGATTACTGTGACTTGGAGTAGGAATTTGATGGGTCAAGGCCTTAGTGTTGGGATAGATGATTCATCAAACCAGTGTCTTTGTAAGGTTGATATTAAACCCTGGTTGTTCTCCAAGAGAAGAGGGTCCAAGAGTTTAGAAGCTTATTCGAGTCAAATAGATATATATTGGGACCTTTCATCGGCAAAATTCGGATCCGGGCCTGAACCTTTGGAGGGATATTATGTCGGGGTTGTGGTGGACAGGCAAATGCTTCTCCTTCTCGGAGACATGAGGAAAGAAGCTTTCAAAAAGACTAGTGCCAACCCTGTGCCTTCGAGTGCTGTTTGCGTTGCTAAGAGAGAGCATATTTTTGGTAAGAGGGGATTCACCACAAAGGCTCAGTTTTGCAATAATGGTCAAGTTCATGACCTTGTAATTGAATGTGACACTGTCGGCGTCAATGATCGATCCTTTACAACTCCTGGTGCGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

203

Amino Acids

22.23

Weight (kDa)

8.88

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 27 - 195 2.9e-68 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 604
AclWI GGATC 3 cut(s) 321, 334, 576
AcsI RAATTY 3 cut(s) 85, 157, 320
AcyI GRCGYC 1 cut(s) 570
AfiI CCNNNNNNNGG 3 cut(s) 88, 313, 347
AgsI TTSAA 2 cut(s) 421, 554
AhlI ACTAGT 1 cut(s) 428
AjnI CCWGG 2 cut(s) 233, 595
AluBI AGCT 2 cut(s) 275, 416
AluI AGCT 2 cut(s) 275, 416
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 3 cut(s) 321, 334, 576
AoxI GGCC 3 cut(s) 121, 172, 332
ApoI RAATTY 3 cut(s) 85, 157, 320
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 258, 304, 332
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 38, 493
AvaII GGWCC 2 cut(s) 258, 304
BaeGI GKGCMC 1 cut(s) 82
BamHI GGATCC 1 cut(s) 326
BanI GGYRCC 1 cut(s) 77
BccI CCATC 2 cut(s) 22, 157
BciT130I CCWGG 2 cut(s) 235, 597
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 393
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 1 cut(s) 429
Bme1390I CCNGG 3 cut(s) 235, 331, 597
Bme18I GGWCC 2 cut(s) 258, 304
BmgT120I GGNCC 3 cut(s) 258, 304, 332
BmiI GGNNCC 4 cut(s) 79, 259, 305, 328
BmrFI CCNGG 3 cut(s) 235, 331, 597
BpuMI CCSGG 1 cut(s) 331
Bsa29I ATCGAT 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 313, 347
Bse118I RCCGGY 2 cut(s) 111, 123
Bse1I ACTGG 1 cut(s) 205
BseBI CCWGG 2 cut(s) 235, 597
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 233
BseLI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 604
BshFI GGCC 3 cut(s) 123, 174, 334
BshNI GGYRCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 580
BsiSI CCGG 3 cut(s) 112, 124, 330
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 123, 174, 334
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 326, 577, 581
BspANI GGCC 3 cut(s) 123, 174, 334
BspCNI CTCAG 1 cut(s) 526
BspDI ATCGAT 1 cut(s) 580
BspFNI CGCG 1 cut(s) 604
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 4 cut(s) 79, 259, 305, 328
BspPI GGATC 3 cut(s) 321, 334, 576
BspT107I GGYRCC 1 cut(s) 77
BsrFI RCCGGY 2 cut(s) 111, 123
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 2 cut(s) 111, 123
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 3 cut(s) 326, 577, 581
BssNI GRCGYC 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 235, 597
Bst4CI ACNGT 2 cut(s) 142, 565
Bst6I CTCTTC 1 cut(s) 247
BstACI GRCGYC 1 cut(s) 570
BstDEI CTNAG 3 cut(s) 176, 468, 513
BstFNI CGCG 1 cut(s) 604
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 3 cut(s) 329, 580, 584
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 3 cut(s) 326, 577, 581
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 2 cut(s) 235, 597
BstSCI CCNGG 3 cut(s) 233, 329, 595
BstSLI GKGCMC 1 cut(s) 82
BstUI CGCG 1 cut(s) 604
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
Bsu15I ATCGAT 1 cut(s) 580
BsuRI GGCC 3 cut(s) 123, 174, 334
BsuTUI ATCGAT 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 212, 561
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 604
Cfr10I RCCGGY 2 cut(s) 111, 123
Cfr13I GGNCC 3 cut(s) 258, 304, 332
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 1 cut(s) 559
CviAII CATG 2 cut(s) 403, 539
CviJI RGCY 6 cut(s) 123, 174, 275, 334, 416, 512
CviKI_1 RGCY 6 cut(s) 123, 174, 275, 334, 416, 512
DdeI CTNAG 3 cut(s) 176, 468, 513
DpnI GATC 3 cut(s) 328, 579, 583
DpnII GATC 3 cut(s) 326, 577, 581
Eam1104I CTCTTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 258, 304
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 233, 595
FaeI CATG 2 cut(s) 406, 542
FaiI YATR 7 cut(s) 101, 296, 298, 357, 404, 480, 540
FaqI GGGAC 1 cut(s) 317
FatI CATG 2 cut(s) 402, 538
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 603
HaeIII GGCC 3 cut(s) 123, 174, 334
HapII CCGG 3 cut(s) 112, 124, 330
HgaI GACGC 1 cut(s) 559
HhaI GCGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 2 cut(s) 406, 542
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HindIII AAGCTT 2 cut(s) 273, 414
HinfI GANTC 4 cut(s) 50, 194, 283, 498
HpaII CCGG 3 cut(s) 112, 124, 330
HphI GGTGA 2 cut(s) 38, 493
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 326, 398
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 401, 456
HpyCH4III ACNGT 2 cut(s) 142, 565
HpyCH4V TGCA 1 cut(s) 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 3 cut(s) 176, 468, 513
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 2 cut(s) 406, 542
HspAI GCGC 1 cut(s) 602
Kzo9I GATC 3 cut(s) 326, 577, 581
MaeI CTAG 1 cut(s) 429
MaeIII GTNAC 4 cut(s) 91, 114, 142, 557
MalI GATC 3 cut(s) 328, 579, 583
MboI GATC 3 cut(s) 326, 577, 581
MboII GAAGA 2 cut(s) 45, 264
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 4 cut(s) 85, 157, 320, 549
MlyI GAGTC 1 cut(s) 292
MnlI CCTC 5 cut(s) 113, 248, 340, 399, 486
MseI TTAA 1 cut(s) 228
MspI CCGG 3 cut(s) 112, 124, 330
MspR9I CCNGG 3 cut(s) 235, 331, 597
MvaI CCWGG 2 cut(s) 235, 597
MvnI CGCG 1 cut(s) 604
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 331
NdeII GATC 3 cut(s) 326, 577, 581
NlaIII CATG 2 cut(s) 406, 542
NlaIV GGNNCC 4 cut(s) 79, 259, 305, 328
NmuCI GTSAC 2 cut(s) 142, 557
PagI TCATGA 1 cut(s) 538
PceI AGGCCT 1 cut(s) 174
PfeI GAWTC 3 cut(s) 50, 194, 498
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 88
PleI GAGTC 1 cut(s) 291
PpsI GAGTC 1 cut(s) 291
PpuMI RGGWCCY 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 2 cut(s) 233, 595
PspGI CCWGG 2 cut(s) 233, 595
PspN4I GGNNCC 4 cut(s) 79, 259, 305, 328
PspPI GGNCC 3 cut(s) 258, 304, 332
PspPPI RGGWCCY 1 cut(s) 304
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 563
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 3 cut(s) 326, 577, 581
Sau96I GGNCC 3 cut(s) 258, 304, 332
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 3 cut(s) 235, 331, 597
SduI GDGCHC 1 cut(s) 82
SetI ASST 7 cut(s) 45, 222, 277, 309, 343, 418, 546
SinI GGWCC 2 cut(s) 258, 304
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 4 cut(s) 85, 157, 320, 549
SseBI AGGCCT 1 cut(s) 174
SspMI CTAG 1 cut(s) 429
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 233, 329, 595
TaaI ACNGT 2 cut(s) 142, 565
TaqI TCGA 3 cut(s) 281, 449, 580
TasI AATT 4 cut(s) 85, 157, 320, 549
TfiI GAWTC 3 cut(s) 50, 194, 498
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 212, 568
TseFI GTSAC 2 cut(s) 142, 557
Tsp45I GTSAC 2 cut(s) 142, 557
TspDTI ATGAA 4 cut(s) 45, 186, 300, 527
TspRI CASTG 2 cut(s) 212, 568
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 258, 304
XapI RAATTY 3 cut(s) 85, 157, 320
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.