MD04G1221100.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004954
Physical Location & Seq
Forward (+)
30212912 .. 30216587
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1221100.v1.1.491

Sequence Viewer

Length: 900 bp
ATGAGAGATTTCCCATCTTGTTTTGGTGAAAATGGGGTTCAGGTTGCTGATTCTTCATCATCAAATAGTAGTAGGACTGCCCAGAATTTGGTTACTTGTGTGTATCAATGCCGGTTACGAGGCCAGTCTTGCTTGATTACTGTGACTTGGAGTAGGAATTTGATGGGTCAAGGCCTTAGTGTTGGGATAGATGATTCATCAAGTCAGTGTCTTTGTAAGGTTGATATTAAACCCTGGTTGTTCTCCAAGAGAAGAGGGTCCAAGAGTTTAGAAGCTTATTCGAGTAAAATCGATATATATTGGGACCTTTCATCGGCAAAATTCGGATCAGGCCCTGAACCTTTGGAGGGATATTATGTGGGAGTTGTGGTGGACAGGCAAATCCTTCTCCTTCTTGGAGACATGAGAAAAGAAGCTTTCAAAAAAACTAGTGTCACCCCTGTGCCTTCGAGTGCCGTTTGCGTTGCTAAGAGAGAGCATATTTTTGGTAAGAGGGGCTTCGCTACAAAGGCTCAGTTTTGCAATAATGGTCAAGTTCATGATCTTGTAATTGAGTGTGACACTGTCGGGGTCAATGATCCATGCCTCCTGGTGCGCGTTGACAGCAAGCCTGTGATGCAGGTAAAGAGACTTCGGTGGAAGTTCCGAGGGAACCATACCATCTTGGTTGATGGGCTTGCTGTTGAAGTTTTCTGGGATGTTCACAATTGGCTCTTTGGTACATCACCTGGAAATGCTGTTTTTATGTTCAAGACATGCCTCTCAGCCGAGAAGCTGTGGGCTAGTCAACCAGCCTCTGATCCAAGCACGTTGCAGTGGTCATTTTCACAGAGATTCTCAGATAGTAAGTCACAAGGTCTAGGATTCTCGCTGATTTTGTATGCTTGGAAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

300

Amino Acids

33.3

Weight (kDa)

8.93

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 27 - 297 3.4e-112 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 597
AclWI GGATC 3 cut(s) 334, 572, 794
AcsI RAATTY 3 cut(s) 85, 157, 320
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 3 cut(s) 88, 313, 347
AgsI TTSAA 3 cut(s) 421, 686, 751
AhlI ACTAGT 1 cut(s) 428
AjnI CCWGG 3 cut(s) 233, 588, 727
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 3 cut(s) 275, 416, 775
AluI AGCT 3 cut(s) 275, 416, 775
Alw26I GTCTC 2 cut(s) 393, 622
AlwI GGATC 3 cut(s) 334, 572, 794
AlwNI CAGNNNCTG 2 cut(s) 335, 797
AoxI GGCC 3 cut(s) 121, 172, 331
ApoI RAATTY 3 cut(s) 85, 157, 320
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 3 cut(s) 258, 304, 332
AsuHPI GGTGA 3 cut(s) 38, 427, 717
AvaII GGWCC 2 cut(s) 258, 304
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BccI CCATC 4 cut(s) 22, 157, 665, 668
BceAI ACGGC 1 cut(s) 440
BciT130I CCWGG 3 cut(s) 235, 590, 729
BcoDI GTCTC 2 cut(s) 393, 622
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 3 cut(s) 429, 783, 860
BfuAI ACCTGC 1 cut(s) 610
Bme1390I CCNGG 3 cut(s) 235, 590, 729
Bme18I GGWCC 2 cut(s) 258, 304
BmgT120I GGNCC 3 cut(s) 258, 304, 332
BmiI GGNNCC 3 cut(s) 259, 305, 653
BmrFI CCNGG 3 cut(s) 235, 590, 729
BmsI GCATC 1 cut(s) 606
Bsa29I ATCGAT 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 233, 646
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 313, 347
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 3 cut(s) 235, 590, 729
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 233, 646
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BseMII CTCAG 3 cut(s) 527, 777, 852
BseNI ACTGG 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 123, 174, 333
BshVI ATCGAT 1 cut(s) 291
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BsmAI GTCTC 2 cut(s) 393, 622
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 123, 174, 333
Bsp143I GATC 4 cut(s) 326, 541, 577, 799
BspANI GGCC 3 cut(s) 123, 174, 333
BspCNI CTCAG 3 cut(s) 526, 776, 851
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 597
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 259, 305, 653
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 3 cut(s) 334, 572, 794
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 233, 646
BssMI GATC 4 cut(s) 326, 541, 577, 799
Bst2UI CCWGG 3 cut(s) 235, 590, 729
Bst4CI ACNGT 2 cut(s) 142, 565
Bst6I CTCTTC 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 608, 678
BstDEI CTNAG 5 cut(s) 176, 468, 513, 763, 838
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 1 cut(s) 597
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 4 cut(s) 329, 544, 580, 802
BstMAI GTCTC 2 cut(s) 393, 622
BstMBI GATC 4 cut(s) 326, 541, 577, 799
BstMWI GCNNNNNNNGC 4 cut(s) 129, 509, 603, 616
BstNI CCWGG 3 cut(s) 235, 590, 729
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 3 cut(s) 233, 588, 727
BstUI CGCG 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 3 cut(s) 123, 174, 333
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 1 cut(s) 703
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 3 cut(s) 212, 561, 821
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 2 cut(s) 608, 678
CaiI CAGNNNCTG 2 cut(s) 335, 797
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 597
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 3 cut(s) 258, 304, 332
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 1 cut(s) 720
CviAII CATG 4 cut(s) 403, 539, 582, 756
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 5 cut(s) 176, 468, 513, 763, 838
DpnI GATC 4 cut(s) 328, 543, 579, 801
DpnII GATC 4 cut(s) 326, 541, 577, 799
Eam1104I CTCTTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 258, 304
EcoO109I RGGNCCY 2 cut(s) 304, 332
EcoRII CCWGG 3 cut(s) 233, 588, 727
FaeI CATG 4 cut(s) 406, 542, 585, 759
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 317
FatI CATG 4 cut(s) 402, 538, 581, 755
FokI GGATG 1 cut(s) 710
FspBI CTAG 3 cut(s) 429, 783, 860
GlaI GCGC 1 cut(s) 596
HaeIII GGCC 3 cut(s) 123, 174, 333
HapII CCGG 1 cut(s) 112
HhaI GCGC 1 cut(s) 597
Hin1II CATG 4 cut(s) 406, 542, 585, 759
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 2 cut(s) 601, 788
HindII GTYRAC 2 cut(s) 601, 788
HindIII AAGCTT 2 cut(s) 273, 414
HinfI GANTC 4 cut(s) 50, 194, 834, 864
HpaII CCGG 1 cut(s) 112
HphI GGTGA 3 cut(s) 38, 427, 717
Hpy166II GTNNAC 4 cut(s) 373, 601, 703, 788
Hpy188I TCNGA 4 cut(s) 326, 647, 799, 841
Hpy188III TCNNGA 2 cut(s) 539, 751
Hpy8I GTNNAC 4 cut(s) 373, 601, 703, 788
HpyAV CCTTC 3 cut(s) 395, 401, 456
HpyCH4III ACNGT 2 cut(s) 142, 565
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 3 cut(s) 522, 619, 814
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 509, 603, 616
HpyF3I CTNAG 5 cut(s) 176, 468, 513, 763, 838
HpySE526I ACGT 1 cut(s) 809
Hsp92II CATG 4 cut(s) 406, 542, 585, 759
HspAI GCGC 1 cut(s) 595
Kzo9I GATC 4 cut(s) 326, 541, 577, 799
LweI GCATC 1 cut(s) 606
MaeI CTAG 3 cut(s) 429, 783, 860
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 6 cut(s) 91, 114, 142, 433, 557, 849
MalI GATC 4 cut(s) 328, 543, 579, 801
MboI GATC 4 cut(s) 326, 541, 577, 799
MboII GAAGA 2 cut(s) 45, 264
MfeI CAATTG 1 cut(s) 706
MluCI AATT 5 cut(s) 85, 157, 320, 549, 706
MnlI CCTC 8 cut(s) 113, 248, 340, 486, 596, 641, 770, 805
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 440
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 3 cut(s) 235, 590, 729
MunI CAATTG 1 cut(s) 706
MvaI CCWGG 3 cut(s) 235, 590, 729
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 4 cut(s) 129, 509, 603, 616
NdeII GATC 4 cut(s) 326, 541, 577, 799
NlaIII CATG 4 cut(s) 406, 542, 585, 759
NlaIV GGNNCC 3 cut(s) 259, 305, 653
NmeAIII GCCGAG 1 cut(s) 793
NmuCI GTSAC 4 cut(s) 142, 433, 557, 849
NspI RCATGY 1 cut(s) 759
OliI CACNNNNGTG 1 cut(s) 440
PagI TCATGA 1 cut(s) 538
PceI AGGCCT 1 cut(s) 174
PfeI GAWTC 4 cut(s) 50, 194, 834, 864
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 233, 588, 727
PspGI CCWGG 3 cut(s) 233, 588, 727
PspN4I GGNNCC 3 cut(s) 259, 305, 653
PspPI GGNCC 3 cut(s) 258, 304, 332
PspPPI RGGWCCY 1 cut(s) 304
PstNI CAGNNNCTG 2 cut(s) 335, 797
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 4 cut(s) 326, 541, 577, 799
Sau96I GGNCC 3 cut(s) 258, 304, 332
ScrFI CCNGG 3 cut(s) 235, 590, 729
SfaNI GCATC 1 cut(s) 606
SinI GGWCC 2 cut(s) 258, 304
SmiMI CAYNNNNRTG 1 cut(s) 440
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 5 cut(s) 85, 157, 320, 549, 706
SseBI AGGCCT 1 cut(s) 174
SspMI CTAG 3 cut(s) 429, 783, 860
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 233, 588, 727
TaaI ACNGT 2 cut(s) 142, 565
TaiI ACGT 1 cut(s) 812
TaqI TCGA 3 cut(s) 281, 291, 449
TasI AATT 5 cut(s) 85, 157, 320, 549, 706
TfiI GAWTC 4 cut(s) 50, 194, 834, 864
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 3 cut(s) 212, 568, 821
TseFI GTSAC 4 cut(s) 142, 433, 557, 849
Tsp45I GTSAC 4 cut(s) 142, 433, 557, 849
TspDTI ATGAA 4 cut(s) 45, 186, 300, 527
TspRI CASTG 3 cut(s) 212, 568, 821
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 258, 304
XapI RAATTY 3 cut(s) 85, 157, 320
XceI RCATGY 1 cut(s) 759
XspI CTAG 3 cut(s) 429, 783, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.