RLG00000025639

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004954
Physical Location & Seq
Forward (+)
47248768 .. 47249676
909 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025639

Sequence Viewer

Length: 909 bp
ATGAGGGATTTCCCATCTTGTTTTGGTGAAAATGGGGTTCAAGTTGCTGATTCTTCTTCGTCGAATAATCATAATACTAGTCGGACTGCGCAGAATTTGGTTACTTGTATCTATCAATGCCGGTTGCGCAGCCGGACTTGCTTGATTACTGTCACATGGAGTAGGAATTTGATGGGTCAAGGCCTTAGTGTTGGGATAGATGATTCGTCAAATCAGTGTCTTTGTAAGGTTGATATTAAACCCTGGTTGTTCTCCAAGAGAAGAGGGTCCAAGAGTTTAGAAGCTTATTCTAGTAAAATTGATATATATTGGGACTTTTCTTCTGCAAAATTTGGATCTGGGCCTGAACCTGTAGACGGATATTACGTGGGAATTGTGGTGGACCGGCAGATGATTCTCCTTCTTGGAGATATGAGAAAGGAAGCTTTTAAAAAGACTAGTGCTAGCCCTGCGTCTTCTAATGCTGTTTGCATTGCCAGGAGAGAGCACATTTTCGGGAAGAAGTTGTTCACCACAAAGGCTCGGTTTTGTAATGATGGTCAAGTTCATGATCTTGTGATTGAATGCGACACTGTTGGGGTCAATGATCCATGCCTTATTGTCCGAGTAGACAGCAAGACGGTGATGCAGGTGAAGAGGCTTCGGTGGAAGTTCCGGGGAAATCACACCATCTTGGTTGATGGGCTTGCAGTTGAAGTTTTCTGGGATGTGCACAATTGGCTCTTTGGTACATCACCTGGAAATGCTGTGTTTATGTTCAAGACATGCCTCTCAGCCGAGAAGTTGTGGTCTGGCCAACCACAATCTGATCCAAGTGCCCTGCAGTGGTCGTTTTCACAGAGATTTTCAGACAGTAAGTCACAAGGGCTAGGATTCTCGCTCATGCTGTATGCTTGGAAGAACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

303

Amino Acids

33.94

Weight (kDa)

9.02

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 30 - 300 4.7e-113 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 619
Acc16I TGCGCA 2 cut(s) 90, 128
Acc36I ACCTGC 1 cut(s) 619
AccI GTMKAC 2 cut(s) 354, 609
AclWI GGATC 3 cut(s) 343, 581, 803
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 3 cut(s) 94, 166, 329
AfaI GTAC 1 cut(s) 730
AfiI CCNNNNNNNGG 2 cut(s) 356, 825
AgsI TTSAA 4 cut(s) 41, 563, 695, 760
AhdI GACNNNNNGTC 1 cut(s) 856
AhlI ACTAGT 2 cut(s) 77, 437
AjnI CCWGG 3 cut(s) 242, 476, 736
AluBI AGCT 2 cut(s) 284, 425
AluI AGCT 2 cut(s) 284, 425
Alw21I GWGCWC 2 cut(s) 489, 714
Alw44I GTGCAC 1 cut(s) 710
AlwI GGATC 3 cut(s) 343, 581, 803
AoxI GGCC 3 cut(s) 181, 341, 793
ApaLI GTGCAC 1 cut(s) 710
ApeKI GCWGC 1 cut(s) 129
ApoI RAATTY 3 cut(s) 94, 166, 329
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 2 cut(s) 91, 129
AspS9I GGNCC 3 cut(s) 267, 341, 382
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 5 cut(s) 38, 502, 634, 643, 726
AsuNHI GCTAGC 1 cut(s) 443
AvaII GGWCC 2 cut(s) 267, 382
BaeGI GKGCMC 2 cut(s) 714, 820
BalI TGGCCA 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 489, 714
BbvI GCAGC 1 cut(s) 141
BccI CCATC 5 cut(s) 22, 166, 530, 674, 677
BciT130I CCWGG 3 cut(s) 244, 478, 738
BcnI CCSGG 1 cut(s) 656
BcuI ACTAGT 2 cut(s) 77, 437
BfaI CTAG 5 cut(s) 78, 291, 438, 444, 869
BfmI CTRYAG 2 cut(s) 351, 821
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
Bme1390I CCNGG 4 cut(s) 244, 478, 656, 738
Bme18I GGWCC 2 cut(s) 267, 382
BmeRI GACNNNNNGTC 1 cut(s) 856
BmgT120I GGNCC 3 cut(s) 267, 341, 382
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 4 cut(s) 244, 478, 656, 738
BmsI GCATC 1 cut(s) 615
BmtI GCTAGC 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 242, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 825
Bse118I RCCGGY 2 cut(s) 120, 384
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 3 cut(s) 244, 478, 738
BseDI CCNNGG 2 cut(s) 242, 655
BseGI GGATG 1 cut(s) 712
BseLI CCNNNNNNNGG 2 cut(s) 356, 825
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 786
BseSI GKGCMC 2 cut(s) 714, 820
BseXI GCAGC 1 cut(s) 141
BshFI GGCC 3 cut(s) 183, 343, 795
BsiHKAI GWGCWC 2 cut(s) 489, 714
BsiSI CCGG 4 cut(s) 121, 133, 385, 655
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 356, 825
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 569
BsnI GGCC 3 cut(s) 183, 343, 795
Bsp1286I GDGCHC 3 cut(s) 489, 714, 820
Bsp143I GATC 4 cut(s) 335, 550, 586, 808
BspANI GGCC 3 cut(s) 183, 343, 795
BspCNI CTCAG 1 cut(s) 785
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 619
BspOI GCTAGC 1 cut(s) 447
BspPI GGATC 3 cut(s) 343, 581, 803
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 2 cut(s) 120, 384
BssAI RCCGGY 2 cut(s) 120, 384
BssECI CCNNGG 2 cut(s) 242, 655
BssMI GATC 4 cut(s) 335, 550, 586, 808
Bst2UI CCWGG 3 cut(s) 244, 478, 738
Bst4CI ACNGT 4 cut(s) 151, 574, 622, 854
Bst6I CTCTTC 2 cut(s) 256, 629
BstBAI YACGTR 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 445, 687
BstDEI CTNAG 2 cut(s) 185, 772
BstF5I GGATG 1 cut(s) 712
BstHHI GCGC 2 cut(s) 91, 129
BstKTI GATC 4 cut(s) 338, 553, 589, 811
BstMBI GATC 4 cut(s) 335, 550, 586, 808
BstMWI GCNNNNNNNGC 4 cut(s) 126, 138, 449, 718
BstNI CCWGG 3 cut(s) 244, 478, 738
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 4 cut(s) 242, 476, 654, 736
BstSFI CTRYAG 2 cut(s) 351, 821
BstSLI GKGCMC 2 cut(s) 714, 820
BstV1I GCAGC 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 3 cut(s) 183, 343, 795
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 1 cut(s) 830
BtsIMutI CAGTG 3 cut(s) 221, 570, 830
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 445, 687
CciI TCATGA 1 cut(s) 547
CfoI GCGC 2 cut(s) 91, 129
Cfr10I RCCGGY 2 cut(s) 120, 384
Cfr13I GGNCC 3 cut(s) 267, 341, 382
CseI GACGC 1 cut(s) 441
Csp6I GTAC 1 cut(s) 729
CviAII CATG 5 cut(s) 156, 548, 591, 765, 883
CviQI GTAC 1 cut(s) 729
DdeI CTNAG 2 cut(s) 185, 772
DpnI GATC 4 cut(s) 337, 552, 588, 810
DpnII GATC 4 cut(s) 335, 550, 586, 808
DraI TTTAAA 1 cut(s) 430
DriI GACNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 256, 629
Eam1105I GACNNNNNGTC 1 cut(s) 856
EarI CTCTTC 2 cut(s) 256, 629
Eco147I AGGCCT 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 267, 382
EcoRII CCWGG 3 cut(s) 242, 476, 736
FaeI CATG 5 cut(s) 159, 551, 594, 768, 886
FaqI GGGAC 1 cut(s) 326
FatI CATG 5 cut(s) 155, 547, 590, 764, 882
FblI GTMKAC 2 cut(s) 354, 609
Fnu4HI GCNGC 1 cut(s) 130
FokI GGATG 1 cut(s) 719
Fsp4HI GCNGC 1 cut(s) 130
FspBI CTAG 5 cut(s) 78, 291, 438, 444, 869
FspI TGCGCA 2 cut(s) 90, 128
GlaI GCGC 2 cut(s) 90, 128
GluI GCNGC 1 cut(s) 130
HaeIII GGCC 3 cut(s) 183, 343, 795
HapII CCGG 4 cut(s) 121, 133, 385, 655
HgaI GACGC 1 cut(s) 441
HhaI GCGC 2 cut(s) 91, 129
Hin1II CATG 5 cut(s) 159, 551, 594, 768, 886
Hin6I GCGC 2 cut(s) 89, 127
HinP1I GCGC 2 cut(s) 89, 127
HindIII AAGCTT 2 cut(s) 282, 423
HinfI GANTC 4 cut(s) 50, 203, 394, 873
HpaII CCGG 4 cut(s) 121, 133, 385, 655
HphI GGTGA 5 cut(s) 38, 502, 634, 643, 726
Hpy166II GTNNAC 5 cut(s) 355, 382, 510, 610, 712
Hpy188I TCNGA 4 cut(s) 84, 605, 808, 850
Hpy188III TCNNGA 3 cut(s) 496, 548, 760
Hpy8I GTNNAC 5 cut(s) 355, 382, 510, 610, 712
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 4 cut(s) 151, 574, 622, 854
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 6 cut(s) 326, 471, 628, 689, 712, 823
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 138, 449, 718
HpyF3I CTNAG 2 cut(s) 185, 772
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 5 cut(s) 159, 551, 594, 768, 886
HspAI GCGC 2 cut(s) 89, 127
Kzo9I GATC 4 cut(s) 335, 550, 586, 808
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 1 cut(s) 615
MaeI CTAG 5 cut(s) 78, 291, 438, 444, 869
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 3 cut(s) 100, 151, 858
MalI GATC 4 cut(s) 337, 552, 588, 810
MboI GATC 4 cut(s) 335, 550, 586, 808
MboII GAAGA 7 cut(s) 45, 48, 273, 312, 447, 511, 646
MfeI CAATTG 1 cut(s) 715
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 3 cut(s) 489, 714, 820
MlsI TGGCCA 1 cut(s) 795
MluCI AATT 6 cut(s) 94, 166, 297, 329, 372, 715
MluNI TGGCCA 1 cut(s) 795
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 3 cut(s) 257, 630, 779
Mox20I TGGCCA 1 cut(s) 795
MroXI GAANNNNTTC 1 cut(s) 506
MscI TGGCCA 1 cut(s) 795
MseI TTAA 2 cut(s) 237, 429
Msp20I TGGCCA 1 cut(s) 795
MspI CCGG 4 cut(s) 121, 133, 385, 655
MspR9I CCNGG 4 cut(s) 244, 478, 656, 738
MunI CAATTG 1 cut(s) 715
Mva1269I GAATGC 1 cut(s) 569
MvaI CCWGG 3 cut(s) 244, 478, 738
MwoI GCNNNNNNNGC 4 cut(s) 126, 138, 449, 718
NciI CCSGG 1 cut(s) 656
NdeII GATC 4 cut(s) 335, 550, 586, 808
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 5 cut(s) 159, 551, 594, 768, 886
NlaIV GGNNCC 1 cut(s) 268
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 2 cut(s) 151, 858
NsbI TGCGCA 2 cut(s) 90, 128
NspI RCATGY 1 cut(s) 768
PagI TCATGA 1 cut(s) 547
PaqCI CACCTGC 1 cut(s) 619
PceI AGGCCT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 1 cut(s) 569
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 4 cut(s) 50, 203, 394, 873
PkrI GCNGC 1 cut(s) 131
Ppu21I YACGTR 1 cut(s) 367
Psp6I CCWGG 3 cut(s) 242, 476, 736
PspGI CCWGG 3 cut(s) 242, 476, 736
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 3 cut(s) 267, 341, 382
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 1 cut(s) 335
RsaI GTAC 1 cut(s) 730
RsaNI GTAC 1 cut(s) 729
SaqAI TTAA 2 cut(s) 237, 429
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 4 cut(s) 335, 550, 586, 808
Sau96I GGNCC 3 cut(s) 267, 341, 382
ScrFI CCNGG 4 cut(s) 244, 478, 656, 738
SduI GDGCHC 3 cut(s) 489, 714, 820
SetI ASST 7 cut(s) 231, 286, 352, 369, 427, 633, 739
SfaNI GCATC 1 cut(s) 615
SfcI CTRYAG 2 cut(s) 351, 821
SinI GGWCC 2 cut(s) 267, 382
SpeI ACTAGT 2 cut(s) 77, 437
Sse9I AATT 6 cut(s) 94, 166, 297, 329, 372, 715
SseBI AGGCCT 1 cut(s) 183
SspMI CTAG 5 cut(s) 78, 291, 438, 444, 869
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 242, 476, 654, 736
TaaI ACNGT 4 cut(s) 151, 574, 622, 854
TaiI ACGT 1 cut(s) 369
TaqI TCGA 1 cut(s) 62
TasI AATT 6 cut(s) 94, 166, 297, 329, 372, 715
TfiI GAWTC 4 cut(s) 50, 203, 394, 873
Tru1I TTAA 2 cut(s) 237, 429
Tru9I TTAA 2 cut(s) 237, 429
TscAI CASTG 3 cut(s) 221, 577, 830
TseFI GTSAC 2 cut(s) 151, 858
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 2 cut(s) 151, 858
TspDTI ATGAA 1 cut(s) 536
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 3 cut(s) 221, 577, 830
VneI GTGCAC 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 267, 382
XapI RAATTY 3 cut(s) 94, 166, 329
XceI RCATGY 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 354, 609
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 5 cut(s) 78, 291, 438, 444, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.