MD12G1237400.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004954
Physical Location & Seq
Forward (+)
31107622 .. 31109787
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1237400.v1.1.491

Sequence Viewer

Length: 900 bp
ATGAGAGATTTCCCATCTTGTTTTGGTGAAAATGGGGTTCAGGTTGCTGATTCTTCATCATCAAATAGTAGTAGGGGTGCCCAGAATTTGGTTACTTGTATGTATCAATGCCGGTTACGAGGCCGGTCTTGCTTGATTACTGTGACTTGGAGTAGGAATTTGATGGGTCAAGGCCTTAGTGTTGGGATAGATGATTCATCAAACCAGTGTCTTTGCAAGGTTGATATTAAACCCTGGTTGTTCTCCAAGAGAAGAGGGTCCAAGAGTTTAGAAGCTTATTCGAGTAAAATAGATATATATTGGGACCTTTCATTGGCAAAATTCGGATCCGGGCCTGAACCTTTGGAGGGATATTATGTGGGGGTTGTGGTGGACAGGCAAATGCTTCTCCTTCTTGGAGACATGAGGAAAGAAGCTTTCAAAAAGACTAGTGCCAGCCCTGTGCCTTCGAGTGCTGTTTGCGTTGCTAAGAGAGAGCATATTTTTGGTAAGAGGGGATTCACCACAAAGGCTCAGTTTTGCAATAATGGTCAAGTTCATGACCTTGTAATTGAATGTGACACTGTCGGGGTCAATGATCCATGCCTCCTGGTGCGCGTTGACAGCAAGCCTGTGATGCAGGTGAAGAGACTTCGGTGGAAGTTCCGAGGGAACCATACCATCTTGGTTGATGGGCTTGCAGTTGAAGTTTTCTGGGATGTTCACAATTGGCTGTTTGGTACATCACCTGGAAATGCTGTTTTTATGTTCAAGACATGCCTCTCAGCAGAGAAGTTGTGGGCTAGTCAACCAGCCTCTGATCCGAGCGCGTTGCAGTGGTCATTTTCGCAGAGATTCTCGGATAGTAAGTCACAAGGTCTAGGATTCTCGCTGATTCTGTATGCTTGGAAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

300

Amino Acids

33.34

Weight (kDa)

9.02

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 27 - 297 2.6e-111 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 610
Acc36I ACCTGC 1 cut(s) 610
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 2 cut(s) 597, 809
AclWI GGATC 4 cut(s) 321, 334, 572, 794
AcsI RAATTY 3 cut(s) 85, 157, 320
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 3 cut(s) 88, 313, 347
AgsI TTSAA 4 cut(s) 421, 554, 686, 751
AhlI ACTAGT 1 cut(s) 428
AjnI CCWGG 3 cut(s) 233, 588, 727
AluBI AGCT 2 cut(s) 275, 416
AluI AGCT 2 cut(s) 275, 416
Alw26I GTCTC 2 cut(s) 393, 622
AlwI GGATC 4 cut(s) 321, 334, 572, 794
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 3 cut(s) 121, 172, 332
ApoI RAATTY 3 cut(s) 85, 157, 320
AspLEI GCGC 2 cut(s) 597, 809
AspS9I GGNCC 3 cut(s) 258, 304, 332
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 4 cut(s) 38, 493, 634, 717
AvaII GGWCC 2 cut(s) 258, 304
BaeGI GKGCMC 1 cut(s) 82
BamHI GGATCC 1 cut(s) 326
BanI GGYRCC 1 cut(s) 77
BccI CCATC 4 cut(s) 22, 157, 665, 668
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 3 cut(s) 235, 590, 729
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 393, 622
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 3 cut(s) 429, 783, 860
BfuAI ACCTGC 1 cut(s) 610
Bme1390I CCNGG 4 cut(s) 235, 331, 590, 729
Bme18I GGWCC 2 cut(s) 258, 304
BmgT120I GGNCC 3 cut(s) 258, 304, 332
BmiI GGNNCC 5 cut(s) 79, 259, 305, 328, 653
BmrFI CCNGG 4 cut(s) 235, 331, 590, 729
BmsI GCATC 1 cut(s) 606
BpuMI CCSGG 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 233, 646
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 313, 347
Bse118I RCCGGY 2 cut(s) 111, 123
Bse1I ACTGG 1 cut(s) 205
BseBI CCWGG 3 cut(s) 235, 590, 729
BseDI CCNNGG 2 cut(s) 233, 646
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BseMII CTCAG 2 cut(s) 527, 777
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 82
Bsh1236I CGCG 2 cut(s) 597, 809
BshFI GGCC 3 cut(s) 123, 174, 334
BshNI GGYRCC 1 cut(s) 77
BsiSI CCGG 3 cut(s) 112, 124, 330
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 88, 313, 347
BsmAI GTCTC 2 cut(s) 393, 622
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 123, 174, 334
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 326, 577, 799
BspANI GGCC 3 cut(s) 123, 174, 334
BspCNI CTCAG 2 cut(s) 526, 776
BspFNI CGCG 2 cut(s) 597, 809
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 5 cut(s) 79, 259, 305, 328, 653
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 4 cut(s) 321, 334, 572, 794
BspT107I GGYRCC 1 cut(s) 77
BsrFI RCCGGY 2 cut(s) 111, 123
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 2 cut(s) 111, 123
BssECI CCNNGG 2 cut(s) 233, 646
BssMI GATC 3 cut(s) 326, 577, 799
Bst2UI CCWGG 3 cut(s) 235, 590, 729
Bst4CI ACNGT 2 cut(s) 142, 565
Bst6I CTCTTC 2 cut(s) 247, 620
BstC8I GCNNGC 3 cut(s) 436, 608, 678
BstDEI CTNAG 4 cut(s) 176, 468, 513, 763
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 2 cut(s) 597, 809
BstHHI GCGC 2 cut(s) 597, 809
BstKTI GATC 3 cut(s) 329, 580, 802
BstMAI GTCTC 2 cut(s) 393, 622
BstMBI GATC 3 cut(s) 326, 577, 799
BstMWI GCNNNNNNNGC 3 cut(s) 129, 603, 616
BstNI CCWGG 3 cut(s) 235, 590, 729
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 4 cut(s) 233, 329, 588, 727
BstSLI GKGCMC 1 cut(s) 82
BstUI CGCG 2 cut(s) 597, 809
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 3 cut(s) 123, 174, 334
BtsCI GGATG 1 cut(s) 703
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 3 cut(s) 212, 561, 821
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 3 cut(s) 436, 608, 678
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 538
CfoI GCGC 2 cut(s) 597, 809
Cfr10I RCCGGY 2 cut(s) 111, 123
Cfr13I GGNCC 3 cut(s) 258, 304, 332
Csp6I GTAC 1 cut(s) 720
CviAII CATG 4 cut(s) 403, 539, 582, 756
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 4 cut(s) 176, 468, 513, 763
DpnI GATC 3 cut(s) 328, 579, 801
DpnII GATC 3 cut(s) 326, 577, 799
Eam1104I CTCTTC 2 cut(s) 247, 620
EarI CTCTTC 2 cut(s) 247, 620
Eco147I AGGCCT 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 258, 304
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 3 cut(s) 233, 588, 727
FaeI CATG 4 cut(s) 406, 542, 585, 759
FaqI GGGAC 1 cut(s) 317
FatI CATG 4 cut(s) 402, 538, 581, 755
FokI GGATG 1 cut(s) 710
FspBI CTAG 3 cut(s) 429, 783, 860
GlaI GCGC 2 cut(s) 596, 808
HaeIII GGCC 3 cut(s) 123, 174, 334
HapII CCGG 3 cut(s) 112, 124, 330
HhaI GCGC 2 cut(s) 597, 809
Hin1II CATG 4 cut(s) 406, 542, 585, 759
Hin6I GCGC 2 cut(s) 595, 807
HinP1I GCGC 2 cut(s) 595, 807
HincII GTYRAC 2 cut(s) 601, 788
HindII GTYRAC 2 cut(s) 601, 788
HindIII AAGCTT 2 cut(s) 273, 414
HinfI GANTC 6 cut(s) 50, 194, 498, 834, 864, 874
HpaII CCGG 3 cut(s) 112, 124, 330
HphI GGTGA 4 cut(s) 38, 493, 634, 717
Hpy166II GTNNAC 4 cut(s) 373, 601, 703, 788
Hpy188I TCNGA 5 cut(s) 326, 647, 799, 804, 841
Hpy188III TCNNGA 2 cut(s) 539, 751
Hpy8I GTNNAC 4 cut(s) 373, 601, 703, 788
HpyAV CCTTC 2 cut(s) 401, 456
HpyCH4III ACNGT 2 cut(s) 142, 565
HpyCH4V TGCA 5 cut(s) 216, 522, 619, 680, 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 603, 616
HpyF3I CTNAG 4 cut(s) 176, 468, 513, 763
Hsp92II CATG 4 cut(s) 406, 542, 585, 759
HspAI GCGC 2 cut(s) 595, 807
Kzo9I GATC 3 cut(s) 326, 577, 799
LweI GCATC 1 cut(s) 606
MaeI CTAG 3 cut(s) 429, 783, 860
MaeIII GTNAC 5 cut(s) 91, 114, 142, 557, 849
MalI GATC 3 cut(s) 328, 579, 801
MboI GATC 3 cut(s) 326, 577, 799
MboII GAAGA 3 cut(s) 45, 264, 637
MfeI CAATTG 1 cut(s) 706
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 5 cut(s) 85, 157, 320, 549, 706
MnlI CCTC 9 cut(s) 113, 248, 340, 399, 486, 596, 641, 770, 805
MseI TTAA 1 cut(s) 228
MspI CCGG 3 cut(s) 112, 124, 330
MspR9I CCNGG 4 cut(s) 235, 331, 590, 729
MunI CAATTG 1 cut(s) 706
MvaI CCWGG 3 cut(s) 235, 590, 729
MvnI CGCG 2 cut(s) 597, 809
MwoI GCNNNNNNNGC 3 cut(s) 129, 603, 616
NciI CCSGG 1 cut(s) 331
NdeII GATC 3 cut(s) 326, 577, 799
NlaIII CATG 4 cut(s) 406, 542, 585, 759
NlaIV GGNNCC 5 cut(s) 79, 259, 305, 328, 653
NmuCI GTSAC 3 cut(s) 142, 557, 849
NspI RCATGY 1 cut(s) 759
PagI TCATGA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 610
PceI AGGCCT 1 cut(s) 174
PfeI GAWTC 6 cut(s) 50, 194, 498, 834, 864, 874
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 233, 588, 727
PspGI CCWGG 3 cut(s) 233, 588, 727
PspN4I GGNNCC 5 cut(s) 79, 259, 305, 328, 653
PspPI GGNCC 3 cut(s) 258, 304, 332
PspPPI RGGWCCY 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 3 cut(s) 326, 577, 799
Sau96I GGNCC 3 cut(s) 258, 304, 332
ScrFI CCNGG 4 cut(s) 235, 331, 590, 729
SduI GDGCHC 1 cut(s) 82
SfaNI GCATC 1 cut(s) 606
SinI GGWCC 2 cut(s) 258, 304
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 5 cut(s) 85, 157, 320, 549, 706
SseBI AGGCCT 1 cut(s) 174
SspMI CTAG 3 cut(s) 429, 783, 860
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 4 cut(s) 233, 329, 588, 727
TaaI ACNGT 2 cut(s) 142, 565
TaqI TCGA 2 cut(s) 281, 449
TasI AATT 5 cut(s) 85, 157, 320, 549, 706
TfiI GAWTC 6 cut(s) 50, 194, 498, 834, 864, 874
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 3 cut(s) 212, 568, 821
TseFI GTSAC 3 cut(s) 142, 557, 849
Tsp45I GTSAC 3 cut(s) 142, 557, 849
TspDTI ATGAA 4 cut(s) 45, 186, 300, 527
TspRI CASTG 3 cut(s) 212, 568, 821
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 258, 304
XapI RAATTY 3 cut(s) 85, 157, 320
XceI RCATGY 1 cut(s) 759
XspI CTAG 3 cut(s) 429, 783, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.