AT5G35180

Protein ENHANCED DISEASE RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001313
Physical Location & Seq
Forward (+)
13424316 .. 13433067
8752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35180.5

Sequence Viewer

Length: 2337 bp
ATGACATCGCCTGGATCGAAGAAAGTTGTTACCACCGACGACGGATCGGAGAAGAAAGTTTCCGGCAATCTCGGAAAAGTAAGTTTTTCCGGAGATCTTAATCATAGTGGAAGCCATAGTGGAAGCCATAGTCGTAGCAGCAGCAGCGCCGGAGGTGGTGAAGGAGGAACGTTTGAGTATTTCGGATGGGTTTATCATTTGGGTGTGAATAAGATCGGTCATGAGTACTGTAATCTTCGTTTCCTTTTCATTAGAGGCAAATATGTTGAGATGTATAAAAGAGATCCTCATGAGAACCCAGACATTAAACCTATAAGGCGTGGTGTCATAGGGCCGACAATGGTGATTGAAGAGCTAGGACGTCGAAAAGTTAATCATGGGGATGTCTACGTTATAAGGTTCTATAACCGGTTGGACGAGTCAAGGAAAGGAGAAATTGCTTGTGCTACTGCTGGAGAAGCCCTGAAATGGGTTGAGGCTTTTGAGGAAGCAAAGCAGCAGGCTGAATATGCACTATCGAGAGGCGGAAGTACAAGAACAAAGTTAAGCATGGAGGCCAACATCGATCTGGAAGGACATCGGCCTAGAGTACGGCGTTATGCTTATGGATTAAAAAAGCTTATCCGAATTGGGCAAGGTCCAGAGTCTCTTTTGAGGCAATCATCTACCCTGGTTAATGATGTCAGGGGTGATGGTTTCTATGAGGGTGGTGACAATGGAGATGCAATCGAGGCACACGAATGGAAATGTGTTCGTACAATCAATGGTGTTAGGATTTTTGAAGATGTTGCTAACTTCAAGGCTGGTCGAGGAGTTCTTGTGAAAGCCGTTGCTGTAGTTGAAGCAAGTGCGGATACCGTGTTCGAAGTACTTTTGAACATTGACAAACATCAAAGATACGAGTGGGATGCAGTAACAGGTGATTCGGAAAAGATAGACTCATACGAGGGTCACTATGATGTCATTTATTGCATATACGATCCTAAATATCTTTCGCGATGGCAGTCAAAGAGAGATTTTGTGTTCTCTAGACAGTGGGTTCGTGGGCAAGATGGGACATATACAATCTTACAGTTTCCAGCAGTGCATAAGAAACGGCCTGCAAAGTCTGGATATCGACGTACAGAAATCACGCCATCAACTTGGGAAATCAAAAGCTTAAAAAAGCGCAGCGATGCAGAAACTCCAAGTTGTCTTGTAACACACATGCTAGAAATCCACTCTAAACGTTGGTGCAAATGGAAGAGGACCAGCTACTCAAAGTTTGAAAAGACTATTCCATATGCATTACTCCTCCAAGTAGCAGGTCTGAAGGAGTACATTGGAGCAAACCCAGCCTTCAAGTATGAAACTTCCGCCACGGTTGTCCAGTCCAAGTTCCAAGATGTCCCCAATGGTGAATATGTAGATGAAGAAATGGAGGAGCAGTTTTATGATGCCACGGACTCATCATCTGGAGAAGAGGATGAGGAGGAAAGCGATGACGATGATGAAAATCAGGACAATAAGGAGATAAAAGTTAAACTGAAGAATGTTTCATGGGCAATCGCTAGCTTATCTTTGAAGCGACCTAAAGCTCCAGGCGCAAGTAATGTATTAGACGCTAGTGTTGATCCTGTGAGTATCGATCCAAGTCAGTTCCAAGGATCTCTGCGCAAAGGAAATGGTGATAAGGACTCAAATTGCTGGAATTCTCCTAGTGGCATGGGGTTTATGATCAGGGGAAAGACCTACCTAAAAGATAATGCAAAGGTGATGGGTGGGCAGCCCCTTCTAACACTTATATCAGTGGATTGGTTCAAAGTTGATAGTGCTGTAGACAACATCGCCCTGCATCCAAAATGTCTTATCCAGTCGGAACCTGGGAAAAAGCTCCCATTCATCCTAGTCATCAATCTACAGGTACCAGCAAAGCCTAACTATTGTCTGGTACTCTACTATGCTGCGGATCGACCTGTAAACAAGACTTCATCCCTGGGAAAATTCGTGGATGGGAGTGACAGTTACCGCGACGCAAGATTTAAGCTAATTCCAAGTATTGTTCAGGGATATTGGATGGTCAAGCGGGCTGTTGGAACAAAAGCCTGCTTGCTCGGTAAAGCTGTGACCTGTAAGTACCTGAGGCAGGATAACTTTTTGGAGATTGATGTAGATATTGGATCATCGGCTGTTGCGAGGAGCGTTATTGGTCTGGTCCTTGGTTATGTCACAAGCCTCATTGTTGATCTCGCCATCTTGATTGAGGGAAAGGAGGAAAGCGATTTGCCCGAGTATATCCTTGGGACAGTTCGACTTAACCGAATAGAGCTGGACTCAGCCGTATCATTTGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336 START

Protein Analysis

778

Amino Acids

87.03

Weight (kDa)

6.96

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 269 - 415 9.2e-07 START domain
EDR2_C PF07059 567 - 773 5.1e-74 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
AatII GACGTC 1 cut(s) 364
Acc16I TGCGCA 1 cut(s) 1655
Acc36I ACCTGC 1 cut(s) 1295
Acc65I GGTACC 1 cut(s) 1903
AccB1I GGYRCC 1 cut(s) 1903
AccI GTMKAC 2 cut(s) 387, 1818
AccII CGCG 2 cut(s) 997, 2010
AccIII TCCGGA 1 cut(s) 89
AciI CCGC 6 cut(s) 525, 851, 1356, 1946, 2008, 2065
AclI AACGTT 2 cut(s) 170, 1228
AclWI GGATC 9 cut(s) 22, 52, 278, 974, 1607, 1622, 1654, 1956, 2167
AcsI RAATTY 2 cut(s) 1690, 1982
AcuI CTGAAG 2 cut(s) 1331, 1547
AcyI GRCGYC 1 cut(s) 361
AfiI CCNNNNNNNGG 3 cut(s) 468, 469, 2125
AgeI ACCGGT 1 cut(s) 408
AgsI TTSAA 9 cut(s) 350, 782, 799, 842, 877, 1268, 1342, 1564, 1801
AjnI CCWGG 5 cut(s) 10, 669, 1579, 1861, 1974
AloI GAACNNNNNNTCC 2 cut(s) 845, 877
Alw26I GTCTC 1 cut(s) 651
AlwI GGATC 9 cut(s) 22, 52, 278, 974, 1607, 1622, 1654, 1956, 2167
Ama87I CYCGRG 1 cut(s) 2267
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 4 cut(s) 332, 555, 581, 1097
ApeKI GCWGC 7 cut(s) 138, 141, 144, 496, 1170, 1765, 1943
ApoI RAATTY 2 cut(s) 1690, 1982
ArsI GACNNNNNNTTYG 2 cut(s) 1023, 1055
AsiGI ACCGGT 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 1903
AspLEI GCGC 4 cut(s) 149, 1170, 1586, 1656
AspS9I GGNCC 4 cut(s) 332, 638, 1248, 2194
AsuHPI GGTGA 8 cut(s) 170, 355, 701, 722, 932, 1409, 1679, 1765
AsuII TTCGAA 1 cut(s) 864
AsuNHI GCTAGC 1 cut(s) 1550
AvaI CYCGRG 1 cut(s) 2267
AvaII GGWCC 3 cut(s) 638, 1248, 2194
AxyI CCTNAGG 1 cut(s) 2120
BanI GGYRCC 1 cut(s) 1903
BbvI GCAGC 7 cut(s) 150, 153, 156, 508, 1182, 1777, 1930
BccI CCATC 9 cut(s) 180, 686, 993, 1046, 1144, 1750, 1985, 2050, 2240
BceAI ACGGC 4 cut(s) 608, 812, 1112, 2303
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 5 cut(s) 12, 671, 1581, 1863, 1976
BciVI GTATCC 1 cut(s) 847
BclI TGATCA 1 cut(s) 1716
BcoDI GTCTC 1 cut(s) 651
BfaI CTAG 8 cut(s) 356, 585, 1029, 1211, 1551, 1605, 1698, 1886
BfmI CTRYAG 3 cut(s) 834, 1815, 1898
BfoI RGCGCY 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 1295
BfuI GTATCC 1 cut(s) 847
BglII AGATCT 1 cut(s) 94
BisI GCNGC 7 cut(s) 139, 142, 145, 497, 1171, 1766, 1944
BlsI GCNGC 7 cut(s) 140, 143, 146, 498, 1172, 1767, 1945
BmcAI AGTACT 2 cut(s) 227, 870
Bme1390I CCNGG 5 cut(s) 12, 671, 1581, 1863, 1976
Bme18I GGWCC 3 cut(s) 638, 1248, 2194
BmeT110I CYCGRG 1 cut(s) 2267
BmgT120I GGNCC 4 cut(s) 332, 638, 1248, 2194
BmiI GGNNCC 2 cut(s) 1860, 1905
BmrFI CCNGG 5 cut(s) 12, 671, 1581, 1863, 1976
BmsI GCATC 5 cut(s) 712, 898, 1165, 1426, 1843
BmtI GCTAGC 1 cut(s) 1554
BplI GAGNNNNNCTC 2 cut(s) 2297, 2329
BpmI CTGGAG 3 cut(s) 474, 1476, 1563
Bpu14I TTCGAA 1 cut(s) 864
Bsa29I ATCGAT 2 cut(s) 564, 1626
BsaBI GATNNNNATC 2 cut(s) 99, 1494
BsaHI GRCGYC 1 cut(s) 361
BsaJI CCNNGG 9 cut(s) 669, 1359, 1440, 1642, 1862, 1974, 1975, 2197, 2278
BsaWI WCCGGW 2 cut(s) 89, 408
BsaXI ACNNNNNCTCC 6 cut(s) 804, 834, 1412, 1442, 1987, 2017
Bsc4I CCNNNNNNNGG 3 cut(s) 468, 469, 2125
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 1369, 1852
Bse21I CCTNAGG 1 cut(s) 2120
Bse8I GATNNNNATC 2 cut(s) 99, 1494
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 5 cut(s) 12, 671, 1581, 1863, 1976
BseCI ATCGAT 2 cut(s) 564, 1626
BseDI CCNNGG 9 cut(s) 669, 1359, 1440, 1642, 1862, 1974, 1975, 2197, 2278
BseGI GGATG 9 cut(s) 191, 388, 913, 1471, 1834, 1881, 1970, 1996, 2061
BseJI GATNNNNATC 2 cut(s) 99, 1494
BseLI CCNNNNNNNGG 3 cut(s) 468, 469, 2125
BseMII CTCAG 2 cut(s) 2111, 2328
BseNI ACTGG 2 cut(s) 1369, 1852
BseRI GAGGAG 5 cut(s) 825, 1283, 1436, 1484, 2191
BseXI GCAGC 7 cut(s) 150, 153, 156, 508, 1182, 1777, 1930
BseYI CCCAGC 1 cut(s) 1333
Bsh1236I CGCG 2 cut(s) 997, 2010
BshFI GGCC 4 cut(s) 334, 557, 583, 1099
BshNI GGYRCC 1 cut(s) 1903
BshTI ACCGGT 1 cut(s) 408
BshVI ATCGAT 2 cut(s) 564, 1626
BsiHKCI CYCGRG 1 cut(s) 2267
BsiSI CCGG 4 cut(s) 63, 90, 150, 409
BslFI GGGAC 3 cut(s) 1069, 1373, 2296
BslI CCNNNNNNNGG 3 cut(s) 468, 469, 2125
BsmAI GTCTC 1 cut(s) 651
BsmFI GGGAC 3 cut(s) 1069, 1373, 2296
BsnI GGCC 4 cut(s) 334, 557, 583, 1099
BsoBI CYCGRG 1 cut(s) 2267
Bsp119I TTCGAA 1 cut(s) 864
Bsp13I TCCGGA 1 cut(s) 89
Bsp68I TCGCGA 1 cut(s) 997
BspACI CCGC 6 cut(s) 525, 851, 1356, 1946, 2008, 2065
BspANI GGCC 4 cut(s) 334, 557, 583, 1099
BspCNI CTCAG 2 cut(s) 2112, 2327
BspDI ATCGAT 2 cut(s) 564, 1626
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 2 cut(s) 997, 2010
BspHI TCATGA 2 cut(s) 220, 289
BspLI GGNNCC 2 cut(s) 1860, 1905
BspMI ACCTGC 1 cut(s) 1295
BspOI GCTAGC 1 cut(s) 1554
BspPI GGATC 9 cut(s) 22, 52, 278, 974, 1607, 1622, 1654, 1956, 2167
BspQI GCTCTTC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 1903
BsrFI RCCGGY 1 cut(s) 408
BsrI ACTGG 2 cut(s) 1369, 1852
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 9 cut(s) 669, 1359, 1440, 1642, 1862, 1974, 1975, 2197, 2278
BssNI GRCGYC 1 cut(s) 361
BssT1I CCWWGG 3 cut(s) 1642, 2197, 2278
Bst2UI CCWGG 5 cut(s) 12, 671, 1581, 1863, 1976
Bst4CI ACNGT 7 cut(s) 230, 859, 1035, 1074, 1363, 2003, 2287
Bst6I CTCTTC 3 cut(s) 345, 1238, 1455
BstACI GRCGYC 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 6 cut(s) 501, 1101, 1552, 2067, 2086, 2090
BstDEI CTNAG 2 cut(s) 2120, 2314
BstDSI CCRYGG 2 cut(s) 1359, 1440
BstENI CCTNNNNNAGG 1 cut(s) 2123
BstF5I GGATG 9 cut(s) 191, 388, 913, 1471, 1834, 1881, 1970, 1996, 2061
BstFNI CGCG 2 cut(s) 997, 2010
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 4 cut(s) 149, 1170, 1586, 1656
BstMAI GTCTC 1 cut(s) 651
BstMWI GCNNNNNNNGC 6 cut(s) 144, 458, 509, 731, 1334, 1583
BstNI CCWGG 5 cut(s) 12, 671, 1581, 1863, 1976
BstNSI RCATGY 1 cut(s) 1210
BstSCI CCNGG 5 cut(s) 10, 669, 1579, 1861, 1974
BstSFI CTRYAG 3 cut(s) 834, 1815, 1898
BstUI CGCG 2 cut(s) 997, 2010
BstV1I GCAGC 7 cut(s) 150, 153, 156, 508, 1182, 1777, 1930
BstX2I RGATCY 3 cut(s) 94, 283, 1646
BstXI CCANNNNNNTGG 1 cut(s) 1143
BstYI RGATCY 3 cut(s) 94, 283, 1646
Bsu15I ATCGAT 2 cut(s) 564, 1626
Bsu36I CCTNAGG 1 cut(s) 2120
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 4 cut(s) 334, 557, 583, 1099
BsuTUI ATCGAT 2 cut(s) 564, 1626
BtgI CCRYGG 2 cut(s) 1359, 1440
BtgZI GCGATG 4 cut(s) 1012, 1188, 1494, 1810
BtsCI GGATG 9 cut(s) 191, 388, 913, 1471, 1834, 1881, 1970, 1996, 2061
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 3 cut(s) 1040, 1089, 1794
BtuMI TCGCGA 1 cut(s) 997
BveI ACCTGC 1 cut(s) 1295
Cac8I GCNNGC 6 cut(s) 501, 1101, 1552, 2067, 2086, 2090
CciI TCATGA 2 cut(s) 220, 289
CfoI GCGC 4 cut(s) 149, 1170, 1586, 1656
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 4 cut(s) 332, 638, 1248, 2194
ClaI ATCGAT 2 cut(s) 564, 1626
CseI GACGC 2 cut(s) 1610, 2021
CspAI ACCGGT 1 cut(s) 408
CviAII CATG 7 cut(s) 221, 290, 377, 550, 1207, 1539, 1705
DdeI CTNAG 2 cut(s) 2120, 2314
Eam1104I CTCTTC 3 cut(s) 345, 1238, 1455
EarI CTCTTC 3 cut(s) 345, 1238, 1455
EciI GGCGGA 2 cut(s) 540, 1345
Eco130I CCWWGG 3 cut(s) 1642, 2197, 2278
Eco32I GATATC 1 cut(s) 1115
Eco47I GGWCC 3 cut(s) 638, 1248, 2194
Eco57I CTGAAG 2 cut(s) 1331, 1547
Eco81I CCTNAGG 1 cut(s) 2120
Eco88I CYCGRG 1 cut(s) 2267
EcoNI CCTNNNNNAGG 1 cut(s) 2123
EcoRI GAATTC 1 cut(s) 1690
EcoRII CCWGG 5 cut(s) 10, 669, 1579, 1861, 1974
EcoRV GATATC 1 cut(s) 1115
EcoT14I CCWWGG 3 cut(s) 1642, 2197, 2278
EcoT22I ATGCAT 1 cut(s) 1288
ErhI CCWWGG 3 cut(s) 1642, 2197, 2278
FaeI CATG 7 cut(s) 224, 293, 380, 553, 1210, 1542, 1708
FaqI GGGAC 3 cut(s) 1069, 1373, 2296
FatI CATG 7 cut(s) 220, 289, 376, 549, 1206, 1538, 1704
FauI CCCGC 1 cut(s) 2058
FauNDI CATATG 1 cut(s) 1282
FbaI TGATCA 1 cut(s) 1716
FblI GTMKAC 2 cut(s) 387, 1818
Fnu4HI GCNGC 7 cut(s) 139, 142, 145, 497, 1171, 1766, 1944
FokI GGATG 9 cut(s) 198, 395, 920, 1478, 1821, 1868, 1957, 2003, 2068
Fsp4HI GCNGC 7 cut(s) 139, 142, 145, 497, 1171, 1766, 1944
FspBI CTAG 8 cut(s) 356, 585, 1029, 1211, 1551, 1605, 1698, 1886
FspI TGCGCA 1 cut(s) 1655
GlaI GCGC 4 cut(s) 148, 1169, 1585, 1655
GluI GCNGC 7 cut(s) 139, 142, 145, 497, 1171, 1766, 1944
GsaI CCCAGC 1 cut(s) 1337
GsuI CTGGAG 3 cut(s) 474, 1476, 1563
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 4 cut(s) 334, 557, 583, 1099
HapII CCGG 4 cut(s) 63, 90, 150, 409
HgaI GACGC 2 cut(s) 1610, 2021
HhaI GCGC 4 cut(s) 149, 1170, 1586, 1656
Hin1I GRCGYC 1 cut(s) 361
Hin1II CATG 7 cut(s) 224, 293, 380, 553, 1210, 1542, 1708
Hin6I GCGC 4 cut(s) 147, 1168, 1584, 1654
HinP1I GCGC 4 cut(s) 147, 1168, 1584, 1654
HindIII AAGCTT 2 cut(s) 617, 1156
HinfI GANTC 7 cut(s) 419, 644, 923, 938, 1445, 1676, 2312
HpaII CCGG 4 cut(s) 63, 90, 150, 409
HphI GGTGA 8 cut(s) 170, 355, 701, 722, 932, 1409, 1679, 1765
Hpy166II GTNNAC 3 cut(s) 388, 1819, 1960
Hpy188I TCNGA 7 cut(s) 49, 74, 185, 626, 928, 1311, 1858
Hpy8I GTNNAC 3 cut(s) 388, 1819, 1960
Hpy99I CGWCG 5 cut(s) 41, 44, 366, 1122, 2015
HpyAV CCTTC 5 cut(s) 155, 566, 1306, 1348, 1781
HpyCH4III ACNGT 7 cut(s) 230, 859, 1035, 1074, 1363, 2003, 2287
HpyCH4IV ACGT 5 cut(s) 170, 361, 390, 1120, 1228
HpyF10VI GCNNNNNNNGC 6 cut(s) 144, 458, 509, 731, 1334, 1583
HpyF3I CTNAG 2 cut(s) 2120, 2314
HpySE526I ACGT 5 cut(s) 170, 361, 390, 1120, 1228
Hsp92I GRCGYC 1 cut(s) 361
Hsp92II CATG 7 cut(s) 224, 293, 380, 553, 1210, 1542, 1708
HspAI GCGC 4 cut(s) 147, 1168, 1584, 1654
Kpn2I TCCGGA 1 cut(s) 89
KpnI GGTACC 1 cut(s) 1907
Ksp22I TGATCA 1 cut(s) 1716
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 5 cut(s) 1325, 1423, 1582, 1878, 2178
Lsp1109I GCAGC 7 cut(s) 150, 153, 156, 508, 1182, 1777, 1930
LweI GCATC 5 cut(s) 712, 898, 1165, 1426, 1843
MaeI CTAG 8 cut(s) 356, 585, 1029, 1211, 1551, 1605, 1698, 1886
MaeII ACGT 5 cut(s) 170, 361, 390, 1120, 1228
MaeIII GTNAC 9 cut(s) 28, 710, 913, 950, 1198, 1997, 2003, 2104, 2206
MboII GAAGA 9 cut(s) 31, 64, 227, 362, 794, 1255, 1424, 1472, 1540
MflI RGATCY 3 cut(s) 94, 283, 1646
MluCI AATT 6 cut(s) 435, 627, 1681, 1690, 1982, 2028
MlyI GAGTC 6 cut(s) 428, 653, 932, 1439, 1670, 2306
MmeI TCCRAC 3 cut(s) 393, 1836, 2053
Mph1103I ATGCAT 1 cut(s) 1288
MroI TCCGGA 1 cut(s) 89
MslI CAYNNNNRTG 4 cut(s) 201, 381, 739, 957
MspI CCGG 4 cut(s) 63, 90, 150, 409
MspR9I CCNGG 5 cut(s) 12, 671, 1581, 1863, 1976
MvaI CCWGG 5 cut(s) 12, 671, 1581, 1863, 1976
MvnI CGCG 2 cut(s) 997, 2010
MwoI GCNNNNNNNGC 6 cut(s) 144, 458, 509, 731, 1334, 1583
NdeI CATATG 1 cut(s) 1282
NheI GCTAGC 1 cut(s) 1550
NlaIII CATG 7 cut(s) 224, 293, 380, 553, 1210, 1542, 1708
NlaIV GGNNCC 2 cut(s) 1860, 1905
NmuCI GTSAC 5 cut(s) 710, 950, 1997, 2104, 2206
NruI TCGCGA 1 cut(s) 997
NsbI TGCGCA 1 cut(s) 1655
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 1 cut(s) 1210
NspV TTCGAA 1 cut(s) 864
PagI TCATGA 2 cut(s) 220, 289
PasI CCCWGGG 1 cut(s) 1975
PciSI GCTCTTC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 4 cut(s) 14, 735, 2171, 2296
PfeI GAWTC 1 cut(s) 923
PinAI ACCGGT 1 cut(s) 408
PkrI GCNGC 7 cut(s) 140, 143, 146, 498, 1172, 1767, 1945
PleI GAGTC 6 cut(s) 427, 652, 932, 1439, 1670, 2306
PpsI GAGTC 6 cut(s) 427, 652, 932, 1439, 1670, 2306
PsiI TTATAA 1 cut(s) 395
Psp1406I AACGTT 2 cut(s) 170, 1228
Psp6I CCWGG 5 cut(s) 10, 669, 1579, 1861, 1974
PspFI CCCAGC 1 cut(s) 1333
PspGI CCWGG 5 cut(s) 10, 669, 1579, 1861, 1974
PspN4I GGNNCC 2 cut(s) 1860, 1905
PspPI GGNCC 4 cut(s) 332, 638, 1248, 2194
PsuI RGATCY 3 cut(s) 94, 283, 1646
RruI TCGCGA 1 cut(s) 997
RseI CAYNNNNRTG 4 cut(s) 201, 381, 739, 957
SapI GCTCTTC 1 cut(s) 345
SatI GCNGC 7 cut(s) 139, 142, 145, 497, 1171, 1766, 1944
Sau96I GGNCC 4 cut(s) 332, 638, 1248, 2194
ScaI AGTACT 2 cut(s) 227, 870
SchI GAGTC 6 cut(s) 428, 653, 932, 1439, 1670, 2306
ScrFI CCNGG 5 cut(s) 12, 671, 1581, 1863, 1976
SfaNI GCATC 5 cut(s) 712, 898, 1165, 1426, 1843
SfcI CTRYAG 3 cut(s) 834, 1815, 1898
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 3 cut(s) 638, 1248, 2194
SmiMI CAYNNNNRTG 4 cut(s) 201, 381, 739, 957
Sse9I AATT 6 cut(s) 435, 627, 1681, 1690, 1982, 2028
SsiI CCGC 6 cut(s) 525, 851, 1356, 1946, 2008, 2065
SspMI CTAG 8 cut(s) 356, 585, 1029, 1211, 1551, 1605, 1698, 1886
StyD4I CCNGG 5 cut(s) 10, 669, 1579, 1861, 1974
StyI CCWWGG 3 cut(s) 1642, 2197, 2278
TaaI ACNGT 7 cut(s) 230, 859, 1035, 1074, 1363, 2003, 2287
TaiI ACGT 5 cut(s) 173, 364, 393, 1123, 1231
TaqII GACCGA 1 cut(s) 206
TasI AATT 6 cut(s) 435, 627, 1681, 1690, 1982, 2028
TatI WGTACW 4 cut(s) 225, 530, 868, 1317
TfiI GAWTC 1 cut(s) 923
TscAI CASTG 3 cut(s) 1040, 1089, 1794
TseFI GTSAC 5 cut(s) 710, 950, 1997, 2104, 2206
TseI GCWGC 7 cut(s) 138, 141, 144, 496, 1170, 1765, 1943
Tsp45I GTSAC 5 cut(s) 710, 950, 1997, 2104, 2206
TspDTI ATGAA 7 cut(s) 238, 1362, 1425, 1506, 1527, 1870, 1959
TspGWI ACGGA 2 cut(s) 57, 1457
TspRI CASTG 3 cut(s) 1040, 1089, 1794
VpaK11BI GGWCC 3 cut(s) 638, 1248, 2194
XagI CCTNNNNNAGG 1 cut(s) 2123
XapI RAATTY 2 cut(s) 1690, 1982
XbaI TCTAGA 1 cut(s) 1028
XceI RCATGY 1 cut(s) 1210
XcmI CCANNNNNNNNNTGG 2 cut(s) 565, 1859
XmiI GTMKAC 2 cut(s) 387, 1818
XspI CTAG 8 cut(s) 356, 585, 1029, 1211, 1551, 1605, 1698, 1886
ZraI GACGTC 1 cut(s) 362
ZrmI AGTACT 2 cut(s) 227, 870
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.