MD02G1283900.v1.1

Protein ENHANCED DISEASE RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001313
Physical Location & Seq
Reverse (-)
34067537 .. 34071475
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1283900.v1.1.491

Sequence Viewer

Length: 2175 bp
ATGTTTGAGTATTTTGGGTGGGTGTATCATCTGGGTGTGAATACGTTAGGGCATGAGTATTGTCACCTTAGGTTTCTCTTCATTAGGGGAAAGTATGTCGAGATGTATAAGCGTGATCCTCATGAGAACCCCGGTATTAGACCCATTCGGAGGGGTGCTGTTGGGCCTACGCTGATGGTGGAGGAGCTGGGGCGCAGAAAGGTTAATCATGGAGGTAAGGGTGGGGATATCTATGTTCTACGGTTTTACAATCGTTTGGATGAAACCAAAAAGGGAGAAATTGCTTGTGCTACTGCCGGAGAAGCACAGAAATGGATGGAGGCATTTGATCAAGCCAAGCAACAGGCTGAGTATGAGCTTTCAAGAGGGGCTAGTGCCAGAAACAAGCTGAACATGGAAGAGGAGATCGATCTTGAAGGTCATCGACCTAGAGTAAGGCACTATGCACATGGATTGAAAAGGCTTATAAAAATTGGGCAAGGCCCGGAGATGCTCTTGCGTCAATCCTCAAGCTTGGGTGGAGATGTTAACACAGAGGGATACTTTCGAACAGATTTTGGAGATGCAGTTGAGGCGTATGTATGGAAATGTGTACGAACAGTTAATGGTGTTCGAATATTTCAAGATGTTGCTAACACTGAGAGAGGAAAAGGCGTTATCGTCAAGTCTGTTGGCGTTATTGAGGCAAGTGCAGATACTGCTTTTGAAGTGATTTTAAACCTCGACCGACACCAGAGATATGAGTGGGATATGCTGACAGCTAACTTAGAGCTGATAGATTCTTATGATGGATACTTTGATGTTGTCTACGGGACTTTTGATCCTATGTATCTTTCTCGGTGGCGTTCCAAGAAAGATTTTCTCTTCTCTAGGCAATGGTTCCGTGGACAAGATGGAACATACACCATCTTGCAATTTCCAGCTGTCCATAAGAAAAAGCCTCAGAGGTCCGGGTATCATCGTACAAAAATAAATCCATCTAGTTGGGAGATTAGAGATCTGAAAACATCCATGGGTTCAAATACTCCACGATGTCTTGTGACGCAAACGGTGGAGATACATTCTGTGGGCTGGTGGAAATGGAAGAAAAATCAGTACTCGAAGTTCGAAAAGGGTGTACCTTATGCATTGTTGTGCCAAGTGGCAGGTTTGAAGGATTATATTGCGTCAAATCCTGCACTAATATTCAAATCATCCGCTTCAGTTATCCAATCGAAGGCAGCTGAAGCTTCCATTTCCAATGCTGAATATGAGGCTGAGACAGTGCATGATGAGTTTTATGATGCAATTTCAGCAGGCTCTTCATCTTCTGATGAAGAGAGCGACAGTGATGAACATAAGAATAAGGAAGCAAAAGTGAGACTGAAGAATGTTGCATGGGCCATCGCTAGCTTAGCTTTGAAGCGAGCTCAAGCTGCAGATGCTAATAAAGAATTTGATTCCAGAGTGCCTGCAATCATTATTGATCCAAGCCAGTTCCATGGTTCCTTGTCCAAAGGGAAGGATGAGACCGTCACAAACTGTTGGACATCTCCAAGTGGCGAGGGATTTATGATCAGAGGAAAGAACTACCTGGAGGACAATTCTAAGGTAATGGGAGGAGATCCCCTACTCAAGCTCATAGCGGTAGATTGGTTCAAAGTTGATAAAAGCATAGATAGGTTCGCACTGCATCCCAGGTGCCTTGTCCAGTCAGAAGCCGGAAAGAAGCTTCCATTTGTACTAGTCTTTAATCTCCAGGTTCCATCTAAACCAAATTATAGTTTGGTACTTTACTATGCTGCTGACAGACCTCCAAATCCAAATTCTTTGTTTGCTAAGTTTGTGGATGGGAGTGACATGTTTCGCAATGCAAGATTAAAGTTAATTCCAAGTATTGCTGAAGGATATTGGATGGTTAAGCGTGCTGTTGGAACTAAAGCTTGCCTACTGGGGAAAGCTGTATCCTGCAAATATCTCAGACAAGACAATTTCCTAGAGATTGATGTAGATATTGGATCATCATCTGTGGCAAGGAGTATCATTGGACTTGTCCTTGGATATGTCACGAGCATAGTGGTTGACCTTGCAATTTTAATAGAGGCAAGGGAGCACGCAGAGTTACCTGAGTACATTTTGGGGACTGCTCGACTAAATCGTTTGAAGCTTGACTCTGCCGTTCATTTGGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336 START

Protein Analysis

725

Amino Acids

81.76

Weight (kDa)

8.71

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 509 - 714 2.4e-74 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 1680
AccI GTMKAC 1 cut(s) 807
AciI CCGC 2 cut(s) 1197, 1625
AclWI GGATC 5 cut(s) 110, 815, 1460, 1598, 2005
AcsI RAATTY 2 cut(s) 1433, 1804
AcuI CTGAAG 4 cut(s) 1185, 1245, 1385, 1902
AfaI GTAC 7 cut(s) 594, 964, 1097, 1119, 1722, 1770, 2111
AfiI CCNNNNNNNGG 2 cut(s) 150, 1216
AflIII ACRYGT 1 cut(s) 1839
AhlI ACTAGT 1 cut(s) 1723
AjnI CCWGG 3 cut(s) 1572, 1676, 1737
AjuI GAANNNNNNNTTGG 2 cut(s) 1789, 1821
Alw21I GWGCWC 2 cut(s) 1411, 2094
Alw26I GTCTC 3 cut(s) 1253, 1354, 1502
AlwI GGATC 5 cut(s) 110, 815, 1460, 1598, 2005
AlwNI CAGNNNCTG 1 cut(s) 698
AoxI GGCC 3 cut(s) 164, 481, 1380
ApeKI GCWGC 3 cut(s) 1220, 1415, 1781
ApoI RAATTY 2 cut(s) 1433, 1804
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 4 cut(s) 164, 482, 948, 1380
AsuC2I CCSGG 3 cut(s) 132, 485, 952
AsuHPI GGTGA 1 cut(s) 56
AsuII TTCGAA 3 cut(s) 547, 613, 1107
AsuNHI GCTAGC 1 cut(s) 1388
AvaII GGWCC 1 cut(s) 948
AxyI CCTNAGG 1 cut(s) 68
BanI GGYRCC 1 cut(s) 1680
BanII GRGCYC 1 cut(s) 1411
BauI CACGAG 1 cut(s) 2047
Bbv12I GWGCWC 2 cut(s) 1411, 2094
BbvI GCAGC 3 cut(s) 1232, 1402, 1768
BceAI ACGGC 1 cut(s) 2141
BciT130I CCWGG 3 cut(s) 1574, 1678, 1739
BciVI GTATCC 3 cut(s) 533, 785, 1954
BclI TGATCA 2 cut(s) 328, 1554
BcnI CCSGG 3 cut(s) 132, 485, 952
BcoDI GTCTC 3 cut(s) 1253, 1354, 1502
BcuI ACTAGT 1 cut(s) 1723
BfaI CTAG 7 cut(s) 372, 429, 870, 981, 1389, 1724, 1976
BfmI CTRYAG 1 cut(s) 1416
BfuAI ACCTGC 1 cut(s) 1136
BfuI GTATCC 3 cut(s) 533, 785, 1954
BglII AGATCT 1 cut(s) 997
BisI GCNGC 3 cut(s) 1221, 1416, 1782
BlpI GCTNAGC 1 cut(s) 1393
BlsI GCNGC 3 cut(s) 1222, 1417, 1783
BmcAI AGTACT 1 cut(s) 1097
Bme1390I CCNGG 6 cut(s) 132, 485, 952, 1574, 1678, 1739
Bme18I GGWCC 1 cut(s) 948
BmgT120I GGNCC 4 cut(s) 164, 482, 948, 1380
BmiI GGNNCC 4 cut(s) 881, 1486, 1682, 1743
BmrFI CCNGG 6 cut(s) 132, 485, 952, 1574, 1678, 1739
BmrI ACTGGG 1 cut(s) 1940
BmsI GCATC 5 cut(s) 480, 553, 1273, 1411, 1681
BmtI GCTAGC 1 cut(s) 1392
BmuI ACTGGG 1 cut(s) 1940
BpmI CTGGAG 2 cut(s) 1595, 1721
Bpu1102I GCTNAGC 1 cut(s) 1393
Bpu14I TTCGAA 3 cut(s) 547, 613, 1107
BpuEI CTTGAG 3 cut(s) 493, 1395, 1598
BpuMI CCSGG 3 cut(s) 132, 485, 952
Bsa29I ATCGAT 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 2002
BsaI GGTCTC 1 cut(s) 1502
BsaJI CCNNGG 6 cut(s) 130, 883, 1011, 1480, 1676, 2035
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 1216
Bse1I ACTGG 3 cut(s) 1474, 1690, 1935
Bse21I CCTNAGG 1 cut(s) 68
Bse3DI GCAATG 2 cut(s) 881, 1855
Bse8I GATNNNNATC 1 cut(s) 2002
BseBI CCWGG 3 cut(s) 1574, 1678, 1739
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 6 cut(s) 130, 883, 1011, 1480, 1676, 2035
BseGI GGATG 8 cut(s) 265, 321, 1007, 1193, 1510, 1672, 1834, 1899
BseJI GATNNNNATC 1 cut(s) 2002
BseLI CCNNNNNNNGG 2 cut(s) 150, 1216
BseMI GCAATG 2 cut(s) 881, 1855
BseMII CTCAG 6 cut(s) 339, 630, 956, 1248, 1972, 2097
BseNI ACTGG 3 cut(s) 1474, 1690, 1935
BseRI GAGGAG 3 cut(s) 197, 416, 1614
BseXI GCAGC 3 cut(s) 1232, 1402, 1768
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 2 cut(s) 711, 1161
Bsh1285I CGRYCG 1 cut(s) 727
BshFI GGCC 3 cut(s) 166, 483, 1382
BshNI GGYRCC 1 cut(s) 1680
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 727
BsiHKAI GWGCWC 2 cut(s) 1411, 2094
BsiSI CCGG 5 cut(s) 132, 297, 485, 951, 1701
BslFI GGGAC 2 cut(s) 826, 2134
BslI CCNNNNNNNGG 2 cut(s) 150, 1216
BsmAI GTCTC 3 cut(s) 1253, 1354, 1502
BsmFI GGGAC 2 cut(s) 826, 2134
BsnI GGCC 3 cut(s) 166, 483, 1382
Bso31I GGTCTC 1 cut(s) 1502
Bsp119I TTCGAA 3 cut(s) 547, 613, 1107
Bsp1286I GDGCHC 2 cut(s) 1411, 2094
Bsp1720I GCTNAGC 1 cut(s) 1393
Bsp19I CCATGG 2 cut(s) 1011, 1480
BspACI CCGC 2 cut(s) 1197, 1625
BspANI GGCC 3 cut(s) 166, 483, 1382
BspCNI CTCAG 6 cut(s) 340, 631, 955, 1249, 1971, 2098
BspDI ATCGAT 1 cut(s) 408
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 4 cut(s) 881, 1486, 1682, 1743
BspMAI CTGCAG 1 cut(s) 1420
BspMI ACCTGC 1 cut(s) 1136
BspOI GCTAGC 1 cut(s) 1392
BspPI GGATC 5 cut(s) 110, 815, 1460, 1598, 2005
BspQI GCTCTTC 1 cut(s) 1306
BspT104I TTCGAA 3 cut(s) 547, 613, 1107
BspT107I GGYRCC 1 cut(s) 1680
BspTNI GGTCTC 1 cut(s) 1502
BsrDI GCAATG 2 cut(s) 881, 1855
BsrI ACTGG 3 cut(s) 1474, 1690, 1935
BssECI CCNNGG 6 cut(s) 130, 883, 1011, 1480, 1676, 2035
BssSI CACGAG 1 cut(s) 2047
BssT1I CCWWGG 3 cut(s) 1011, 1480, 2035
Bst2BI CACGAG 1 cut(s) 2047
Bst2UI CCWGG 3 cut(s) 1574, 1678, 1739
Bst4CI ACNGT 7 cut(s) 243, 601, 1051, 1264, 1328, 1513, 1523
Bst6I CTCTTC 5 cut(s) 83, 393, 869, 1306, 1311
BstAPI GCANNNNNTGC 1 cut(s) 698
BstBI TTCGAA 3 cut(s) 547, 613, 1107
BstC8I GCNNGC 7 cut(s) 1297, 1390, 1407, 1452, 1905, 1924, 2094
BstDSI CCRYGG 3 cut(s) 883, 1011, 1480
BstF5I GGATG 8 cut(s) 265, 321, 1007, 1193, 1510, 1672, 1834, 1899
BstHHI GCGC 1 cut(s) 195
BstMAI GTCTC 3 cut(s) 1253, 1354, 1502
BstMCI CGRYCG 1 cut(s) 727
BstMWI GCNNNNNNNGC 8 cut(s) 302, 572, 698, 1226, 1292, 1394, 1415, 1421
BstNI CCWGG 3 cut(s) 1574, 1678, 1739
BstNSI RCATGY 1 cut(s) 1843
BstSCI CCNGG 6 cut(s) 130, 483, 950, 1572, 1676, 1737
BstSFI CTRYAG 1 cut(s) 1416
BstV1I GCAGC 3 cut(s) 1232, 1402, 1768
BstX2I RGATCY 2 cut(s) 997, 1603
BstXI CCANNNNNNTGG 2 cut(s) 984, 1481
BstYI RGATCY 2 cut(s) 997, 1603
Bsu15I ATCGAT 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 68
BsuI GTATCC 3 cut(s) 533, 785, 1954
BsuRI GGCC 3 cut(s) 166, 483, 1382
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 3 cut(s) 883, 1011, 1480
BtgZI GCGATG 1 cut(s) 1369
BtsCI GGATG 8 cut(s) 265, 321, 1007, 1193, 1510, 1672, 1834, 1899
BtsI GCAGTG 1 cut(s) 1667
BtsIMutI CAGTG 4 cut(s) 636, 1269, 1333, 1667
BveI ACCTGC 1 cut(s) 1136
Cac8I GCNNGC 7 cut(s) 1297, 1390, 1407, 1452, 1905, 1924, 2094
CaiI CAGNNNCTG 1 cut(s) 698
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 164, 482, 948, 1380
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 3 cut(s) 488, 1051, 1155
Csp6I GTAC 7 cut(s) 593, 963, 1096, 1118, 1721, 1769, 2110
CviQI GTAC 7 cut(s) 593, 963, 1096, 1118, 1721, 1769, 2110
DraI TTTAAA 1 cut(s) 717
Eam1104I CTCTTC 5 cut(s) 83, 393, 869, 1306, 1311
EarI CTCTTC 5 cut(s) 83, 393, 869, 1306, 1311
Ecl136II GAGCTC 1 cut(s) 1409
Eco130I CCWWGG 3 cut(s) 1011, 1480, 2035
Eco24I GRGCYC 1 cut(s) 1411
Eco31I GGTCTC 1 cut(s) 1502
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 948
Eco53kI GAGCTC 1 cut(s) 1409
Eco57I CTGAAG 4 cut(s) 1185, 1245, 1385, 1902
Eco81I CCTNAGG 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 1409
EcoRII CCWGG 3 cut(s) 1572, 1676, 1737
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 3 cut(s) 1011, 1480, 2035
EcoT22I ATGCAT 1 cut(s) 1129
EcoT38I GRGCYC 1 cut(s) 1411
ErhI CCWWGG 3 cut(s) 1011, 1480, 2035
FaqI GGGAC 2 cut(s) 826, 2134
FbaI TGATCA 2 cut(s) 328, 1554
FblI GTMKAC 1 cut(s) 807
Fnu4HI GCNGC 3 cut(s) 1221, 1416, 1782
FokI GGATG 8 cut(s) 272, 328, 994, 1180, 1517, 1659, 1841, 1906
FriOI GRGCYC 1 cut(s) 1411
Fsp4HI GCNGC 3 cut(s) 1221, 1416, 1782
FspBI CTAG 7 cut(s) 372, 429, 870, 981, 1389, 1724, 1976
GlaI GCGC 1 cut(s) 194
GluI GCNGC 3 cut(s) 1221, 1416, 1782
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 1595, 1721
HaeIII GGCC 3 cut(s) 166, 483, 1382
HapII CCGG 5 cut(s) 132, 297, 485, 951, 1701
HgaI GACGC 3 cut(s) 488, 1051, 1155
HhaI GCGC 1 cut(s) 195
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 2 cut(s) 529, 2062
HindII GTYRAC 2 cut(s) 529, 2062
HindIII AAGCTT 5 cut(s) 511, 1227, 1709, 1920, 2144
HinfI GANTC 3 cut(s) 779, 1439, 2150
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 5 cut(s) 132, 297, 485, 951, 1701
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 6 cut(s) 529, 593, 808, 887, 1118, 2062
Hpy188I TCNGA 7 cut(s) 150, 945, 1002, 1312, 1559, 1696, 1961
Hpy188III TCNNGA 7 cut(s) 100, 122, 363, 413, 623, 1443, 2047
Hpy8I GTNNAC 6 cut(s) 529, 593, 808, 887, 1118, 2062
HpyAV CCTTC 5 cut(s) 410, 1147, 1210, 1495, 1877
HpyCH4III ACNGT 7 cut(s) 243, 601, 1051, 1264, 1328, 1513, 1523
HpyCH4IV ACGT 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 8 cut(s) 302, 572, 698, 1226, 1292, 1394, 1415, 1421
HpySE526I ACGT 1 cut(s) 44
HspAI GCGC 1 cut(s) 193
Ksp22I TGATCA 2 cut(s) 328, 1554
KspAI GTTAAC 1 cut(s) 529
LguI GCTCTTC 1 cut(s) 1306
LmnI GCTCC 2 cut(s) 184, 2089
Lsp1109I GCAGC 3 cut(s) 1232, 1402, 1768
LweI GCATC 5 cut(s) 480, 553, 1273, 1411, 1681
MaeI CTAG 7 cut(s) 372, 429, 870, 981, 1389, 1724, 1976
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 6 cut(s) 62, 1039, 1513, 1835, 2044, 2100
MboII GAAGA 8 cut(s) 70, 410, 856, 1096, 1293, 1299, 1328, 1378
MflI RGATCY 2 cut(s) 997, 1603
MhlI GDGCHC 2 cut(s) 1411, 2094
MlyI GAGTC 1 cut(s) 2144
MmeI TCCRAC 2 cut(s) 1505, 1891
Mph1103I ATGCAT 1 cut(s) 1129
MslI CAYNNNNRTG 3 cut(s) 33, 310, 1132
MspA1I CMGCKG 2 cut(s) 923, 1223
MspI CCGG 5 cut(s) 132, 297, 485, 951, 1701
MspR9I CCNGG 6 cut(s) 132, 485, 952, 1574, 1678, 1739
MvaI CCWGG 3 cut(s) 1574, 1678, 1739
MwoI GCNNNNNNNGC 8 cut(s) 302, 572, 698, 1226, 1292, 1394, 1415, 1421
NciI CCSGG 3 cut(s) 132, 485, 952
NcoI CCATGG 2 cut(s) 1011, 1480
NheI GCTAGC 1 cut(s) 1388
NlaIV GGNNCC 4 cut(s) 881, 1486, 1682, 1743
NmuCI GTSAC 5 cut(s) 62, 1039, 1513, 1835, 2044
NsiI ATGCAT 1 cut(s) 1129
NspI RCATGY 1 cut(s) 1843
NspV TTCGAA 3 cut(s) 547, 613, 1107
PagI TCATGA 1 cut(s) 121
PciI ACATGT 1 cut(s) 1839
PciSI GCTCTTC 1 cut(s) 1306
PcsI WCGNNNNNNNCGW 1 cut(s) 2134
PfeI GAWTC 2 cut(s) 779, 1439
PkrI GCNGC 3 cut(s) 1222, 1417, 1783
PleI GAGTC 1 cut(s) 2144
PpsI GAGTC 1 cut(s) 2144
PscI ACATGT 1 cut(s) 1839
PsiI TTATAA 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 1411
Psp6I CCWGG 3 cut(s) 1572, 1676, 1737
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 3 cut(s) 1572, 1676, 1737
PspN4I GGNNCC 4 cut(s) 881, 1486, 1682, 1743
PspPI GGNCC 4 cut(s) 164, 482, 948, 1380
PsrI GAACNNNNNNTAC 2 cut(s) 1088, 1120
PstI CTGCAG 1 cut(s) 1420
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 2 cut(s) 997, 1603
PvuII CAGCTG 2 cut(s) 923, 1223
RsaI GTAC 7 cut(s) 594, 964, 1097, 1119, 1722, 1770, 2111
RsaNI GTAC 7 cut(s) 593, 963, 1096, 1118, 1721, 1769, 2110
RseI CAYNNNNRTG 3 cut(s) 33, 310, 1132
SacI GAGCTC 1 cut(s) 1411
SapI GCTCTTC 1 cut(s) 1306
SatI GCNGC 3 cut(s) 1221, 1416, 1782
Sau96I GGNCC 4 cut(s) 164, 482, 948, 1380
ScaI AGTACT 1 cut(s) 1097
SchI GAGTC 1 cut(s) 2144
ScrFI CCNGG 6 cut(s) 132, 485, 952, 1574, 1678, 1739
SduI GDGCHC 2 cut(s) 1411, 2094
SfaNI GCATC 5 cut(s) 480, 553, 1273, 1411, 1681
SfcI CTRYAG 1 cut(s) 1416
SfuI TTCGAA 3 cut(s) 547, 613, 1107
SinI GGWCC 1 cut(s) 948
SmiMI CAYNNNNRTG 3 cut(s) 33, 310, 1132
SmlI CTYRAG 3 cut(s) 508, 1410, 1613
SmoI CTYRAG 3 cut(s) 508, 1410, 1613
SpeI ACTAGT 1 cut(s) 1723
SsiI CCGC 2 cut(s) 1197, 1625
SspI AATATT 2 cut(s) 618, 1185
SspMI CTAG 7 cut(s) 372, 429, 870, 981, 1389, 1724, 1976
SstI GAGCTC 1 cut(s) 1411
StyD4I CCNGG 6 cut(s) 130, 483, 950, 1572, 1676, 1737
StyI CCWWGG 3 cut(s) 1011, 1480, 2035
TaaI ACNGT 7 cut(s) 243, 601, 1051, 1264, 1328, 1513, 1523
TaiI ACGT 1 cut(s) 47
TaqII GACCGA 1 cut(s) 741
TatI WGTACW 3 cut(s) 1095, 1720, 2109
TfiI GAWTC 2 cut(s) 779, 1439
TscAI CASTG 4 cut(s) 643, 1269, 1333, 1674
TseFI GTSAC 5 cut(s) 62, 1039, 1513, 1835, 2044
TseI GCWGC 3 cut(s) 1220, 1415, 1781
Tsp45I GTSAC 5 cut(s) 62, 1039, 1513, 1835, 2044
TspDTI ATGAA 6 cut(s) 70, 276, 1293, 1329, 1347, 2150
TspGWI ACGGA 1 cut(s) 872
TspRI CASTG 4 cut(s) 643, 1269, 1333, 1674
VpaK11BI GGWCC 1 cut(s) 948
XapI RAATTY 2 cut(s) 1433, 1804
XceI RCATGY 1 cut(s) 1843
XmiI GTMKAC 1 cut(s) 807
XspI CTAG 7 cut(s) 372, 429, 870, 981, 1389, 1724, 1976
ZrmI AGTACT 1 cut(s) 1097
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.