AT5G37530

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005059
Physical Location & Seq
Forward (+)
14906442 .. 14909266
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37530.2

Sequence Viewer

Length: 1374 bp
ATGGAGGAGAAATCAAAGTATCTAAGTCTCGTCGGAGCCGGAGCTCTTATCGGATCCATCTCCACCGTCGCTCTCCTTAAACTTCTCTCCAGGAGTTCATTGAAGCAGCTTAATGAAAACCCAGAAACTAATTTCTTAGCAGGAAATGGTATTGAATCTGAAACAAGACCAGATACTGTAGCAAATGGTCAAGATCTTTTGAAAGATGAAATTGTTTCTGAACATTTAACCAGGAATATACAGTTTTTCGGTCTTGAATCGCAGCATAAAGTAACTGGATCATATGTTGTGGTTATTGGACTTGGAGGTGTAGGGAGTCATGCTGCATCTATGCTCTTGAGGTCAGGTGTTGGCAAGCTTCTCCTTGTTGACTTTGATCAGGTGTCACTATCATCATTAAATCGACACGCTGTTGCAACACGAGCAGATGTTGGTATTCCGAAAGCTATGTGTCTCAAGAAGCATTTCTCTTCAATCTTTCCTGAATGCCATATAGAAGCCAAGGTGATGCTGTATGACTCATCATCTGAAGAAGAAATTCTTTCAGGCAAGCCAGATTTCGTTCTGGACTGCATTGACAACATCGATACAAAGGTGGGACTTTTGGCTGCATGTGTTAAGAGGGGTTTGAAAGTTCTGAGTGCAACTGGTGCTGGGGCTAGAGCTGACCCAACAAGAATCAGAGTGGCTGATATAAGAGAGTCAACAATTGACCCGTTATCTCGATCTGTAAGACACAGATTGAGGAGAAAACACGGCATTGAAGGAGGCATTCCTGTTGTATTTTCCCTGGAAAAACCAAAAGCAAAGCTGCTTCCCTTTGAGGGGACAAATGGAGAAGACGAGAATCCTTTAGATTATCAAGTTGTTCCTGGCTTTCGTGTTCGGATAATTCCTGTTCTCGGAACCATCCCGGCTATATTTGGACAAATTATGGCCTCCTATGTTATAACACAACTTGCAGGTGTGCAAGTTCAGATGGAGCCTATAGTGAATCTAGATTTGGATCATTACCGATTGCTTCATCAACGCCTTATTGAGCATGAGGAGACAGTTTATGGCACATCTGCTGAAGTCGAGGTGGATGTTGAGGAAGTGATGTACATAGTGAAAGAGTTGTGGCACGGACGAAGTGCTAGAGATGAGACTGCAAAAGATGTTGGCAGAGGGATGTGGCGAGCCATGAATGAGTTGATGCTCGTAAGATGGGATGCAAAAAAGCCAGCAACAGTCTCAAACTTGGTTCTTTTGAAGTTCAAAGAGGCGGATGAACATGAGACTAAGACTCTTGAGGAAGTGAAGGAAAGTGAAACAGAGTTCTTTGAAAGAGTTTCATGCGTACTGAAGAAGGCAGAGCTTGATTTCTACGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

457

Amino Acids

50.56

Weight (kDa)

5.8

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 76 - 334 7.2e-45 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 950
AarI CACCTGC 1 cut(s) 953
Acc36I ACCTGC 1 cut(s) 953
AciI CCGC 1 cut(s) 1265
AclWI GGATC 4 cut(s) 48, 61, 286, 1014
AcsI RAATTY 1 cut(s) 537
AcuI CTGAAG 3 cut(s) 549, 1092, 1364
AfaI GTAC 2 cut(s) 1103, 1341
AfiI CCNNNNNNNGG 3 cut(s) 824, 825, 902
AjnI CCWGG 4 cut(s) 89, 230, 789, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
AluBI AGCT 7 cut(s) 44, 109, 358, 446, 665, 811, 1357
AluI AGCT 7 cut(s) 44, 109, 358, 446, 665, 811, 1357
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 6 cut(s) 32, 458, 1043, 1139, 1237, 1271
AlwI GGATC 4 cut(s) 48, 61, 286, 1014
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 1 cut(s) 936
ApeKI GCWGC 5 cut(s) 106, 262, 323, 608, 811
ApoI RAATTY 1 cut(s) 537
Asp700I GAANNNNTTC 2 cut(s) 464, 537
AsuC2I CCSGG 1 cut(s) 914
AsuHPI GGTGA 1 cut(s) 517
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BamHI GGATCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 46
BauI CACGAG 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 5 cut(s) 118, 274, 310, 595, 798
BccI CCATC 4 cut(s) 65, 917, 973, 1200
BceAI ACGGC 1 cut(s) 772
BciT130I CCWGG 4 cut(s) 91, 232, 791, 873
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 6 cut(s) 32, 458, 1043, 1139, 1237, 1271
BfaI CTAG 4 cut(s) 660, 998, 1137, 1372
BfmI CTRYAG 2 cut(s) 177, 987
BfuAI ACCTGC 1 cut(s) 953
BglII AGATCT 1 cut(s) 193
BisI GCNGC 5 cut(s) 107, 263, 324, 609, 812
BlsI GCNGC 5 cut(s) 108, 264, 325, 610, 813
Bme1390I CCNGG 5 cut(s) 91, 232, 791, 873, 914
BmiI GGNNCC 4 cut(s) 37, 55, 907, 984
BmrFI CCNGG 5 cut(s) 91, 232, 791, 873, 914
BmsI GCATC 4 cut(s) 335, 498, 1185, 1201
BpiI GAAGAC 1 cut(s) 846
BpmI CTGGAG 1 cut(s) 73
BpuEI CTTGAG 3 cut(s) 358, 440, 1310
BpuMI CCSGG 1 cut(s) 914
Bsa29I ATCGAT 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 2 cut(s) 501, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 824, 825, 902
Bse1I ACTGG 2 cut(s) 280, 652
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 4 cut(s) 91, 232, 791, 873
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 2 cut(s) 501, 789
BseGI GGATG 5 cut(s) 909, 1090, 1176, 1216, 1273
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 3 cut(s) 824, 825, 902
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 2 cut(s) 280, 652
BseRI GAGGAG 3 cut(s) 20, 760, 1061
BseXI GCAGC 5 cut(s) 118, 274, 310, 595, 798
BseYI CCCAGC 1 cut(s) 653
BshFI GGCC 1 cut(s) 938
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 2 cut(s) 39, 914
BslFI GGGAC 2 cut(s) 612, 841
BslI CCNNNNNNNGG 3 cut(s) 824, 825, 902
BsmAI GTCTC 6 cut(s) 32, 458, 1043, 1139, 1237, 1271
BsmFI GGGAC 2 cut(s) 612, 841
BsmI GAATGC 2 cut(s) 491, 771
BsnI GGCC 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
BspACI CCGC 1 cut(s) 1265
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 1 cut(s) 630
BspDI ATCGAT 1 cut(s) 585
BspLI GGNNCC 4 cut(s) 37, 55, 907, 984
BspMI ACCTGC 1 cut(s) 953
BspPI GGATC 4 cut(s) 48, 61, 286, 1014
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 2 cut(s) 280, 652
BssECI CCNNGG 2 cut(s) 501, 789
BssMI GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 4 cut(s) 91, 232, 791, 873
Bst4CI ACNGT 5 cut(s) 67, 178, 243, 1054, 1231
Bst6I CTCTTC 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 650
BstAUI TGTACA 1 cut(s) 1101
BstC8I GCNNGC 4 cut(s) 356, 551, 1179, 1224
BstDEI CTNAG 4 cut(s) 23, 136, 638, 1281
BstF5I GGATG 5 cut(s) 909, 1090, 1176, 1216, 1273
BstKTI GATC 6 cut(s) 56, 196, 281, 379, 728, 1009
BstMAI GTCTC 6 cut(s) 32, 458, 1043, 1139, 1237, 1271
BstMBI GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
BstMWI GCNNNNNNNGC 2 cut(s) 422, 650
BstNI CCWGG 4 cut(s) 91, 232, 791, 873
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 5 cut(s) 89, 230, 789, 871, 912
BstSFI CTRYAG 2 cut(s) 177, 987
BstV1I GCAGC 5 cut(s) 118, 274, 310, 595, 798
BstV2I GAAGAC 1 cut(s) 846
BstX2I RGATCY 2 cut(s) 53, 193
BstYI RGATCY 2 cut(s) 53, 193
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 1 cut(s) 938
BsuTUI ATCGAT 1 cut(s) 585
BtsCI GGATG 5 cut(s) 909, 1090, 1176, 1216, 1273
BveI ACCTGC 1 cut(s) 953
Cac8I GCNNGC 4 cut(s) 356, 551, 1179, 1224
CaiI CAGNNNCTG 1 cut(s) 176
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 2 cut(s) 1102, 1340
CviAII CATG 6 cut(s) 320, 612, 1043, 1183, 1274, 1335
CviQI GTAC 2 cut(s) 1102, 1340
DdeI CTNAG 4 cut(s) 23, 136, 638, 1281
DpnI GATC 6 cut(s) 55, 195, 280, 378, 727, 1008
DpnII GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
EciI GGCGGA 1 cut(s) 1280
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 3 cut(s) 549, 1092, 1364
EcoICRI GAGCTC 1 cut(s) 44
EcoRII CCWGG 4 cut(s) 89, 230, 789, 871
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 6 cut(s) 323, 615, 1046, 1186, 1277, 1338
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 2 cut(s) 612, 841
FatI CATG 6 cut(s) 319, 611, 1042, 1182, 1273, 1334
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 5 cut(s) 107, 263, 324, 609, 812
FokI GGATG 5 cut(s) 896, 1097, 1183, 1223, 1280
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 5 cut(s) 107, 263, 324, 609, 812
FspBI CTAG 4 cut(s) 660, 998, 1137, 1372
GluI GCNGC 5 cut(s) 107, 263, 324, 609, 812
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 1 cut(s) 938
HapII CCGG 2 cut(s) 39, 914
Hin1II CATG 6 cut(s) 323, 615, 1046, 1186, 1277, 1338
HincII GTYRAC 2 cut(s) 370, 705
HindII GTYRAC 2 cut(s) 370, 705
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 9 cut(s) 155, 257, 316, 518, 678, 701, 847, 994, 1285
HpaII CCGG 2 cut(s) 39, 914
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 2 cut(s) 370, 705
Hpy188III TCNNGA 9 cut(s) 191, 254, 337, 457, 482, 566, 723, 998, 1289
Hpy8I GTNNAC 2 cut(s) 370, 705
Hpy99I CGWCG 2 cut(s) 35, 71
HpyAV CCTTC 3 cut(s) 758, 1294, 1342
HpyCH4III ACNGT 5 cut(s) 67, 178, 243, 1054, 1231
HpyCH4V TGCA 9 cut(s) 326, 416, 573, 611, 644, 962, 970, 1151, 1214
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 650
HpyF3I CTNAG 4 cut(s) 23, 136, 638, 1281
Hsp92II CATG 6 cut(s) 323, 615, 1046, 1186, 1277, 1338
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
LmnI GCTCC 3 cut(s) 35, 41, 982
Lsp1109I GCAGC 5 cut(s) 118, 274, 310, 595, 798
LweI GCATC 4 cut(s) 335, 498, 1185, 1201
MaeI CTAG 4 cut(s) 660, 998, 1137, 1372
MaeIII GTNAC 2 cut(s) 271, 384
MalI GATC 6 cut(s) 55, 195, 280, 378, 727, 1008
MboI GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
MboII GAAGA 5 cut(s) 462, 542, 545, 851, 1357
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 2 cut(s) 53, 193
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 6 cut(s) 130, 210, 537, 708, 891, 930
MlyI GAGTC 4 cut(s) 325, 512, 710, 1279
MmeI TCCRAC 1 cut(s) 13
MroXI GAANNNNTTC 2 cut(s) 464, 537
MseI TTAA 5 cut(s) 78, 111, 227, 398, 618
MspI CCGG 2 cut(s) 39, 914
MspR9I CCNGG 5 cut(s) 91, 232, 791, 873, 914
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 2 cut(s) 491, 771
MvaI CCWGG 4 cut(s) 91, 232, 791, 873
MwoI GCNNNNNNNGC 2 cut(s) 422, 650
NciI CCSGG 1 cut(s) 914
NdeI CATATG 1 cut(s) 283
NdeII GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
NlaIII CATG 6 cut(s) 323, 615, 1046, 1186, 1277, 1338
NlaIV GGNNCC 4 cut(s) 37, 55, 907, 984
NmuCI GTSAC 1 cut(s) 384
NspI RCATGY 1 cut(s) 615
PaqCI CACCTGC 1 cut(s) 953
PctI GAATGC 2 cut(s) 491, 771
PdmI GAANNNNTTC 2 cut(s) 464, 537
PfeI GAWTC 5 cut(s) 155, 257, 678, 847, 994
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 5 cut(s) 108, 264, 325, 610, 813
PleI GAGTC 4 cut(s) 324, 512, 709, 1279
PpsI GAGTC 4 cut(s) 324, 512, 709, 1279
PsiI TTATAA 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 4 cut(s) 89, 230, 789, 871
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 4 cut(s) 89, 230, 789, 871
PspN4I GGNNCC 4 cut(s) 37, 55, 907, 984
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 2 cut(s) 53, 193
RsaI GTAC 2 cut(s) 1103, 1341
RsaNI GTAC 2 cut(s) 1102, 1340
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 5 cut(s) 78, 111, 227, 398, 618
SatI GCNGC 5 cut(s) 107, 263, 324, 609, 812
Sau3AI GATC 6 cut(s) 53, 193, 278, 376, 725, 1006
SchI GAGTC 4 cut(s) 325, 512, 710, 1279
ScrFI CCNGG 5 cut(s) 91, 232, 791, 873, 914
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 4 cut(s) 335, 498, 1185, 1201
SfcI CTRYAG 2 cut(s) 177, 987
SmlI CTYRAG 3 cut(s) 337, 455, 1289
SmoI CTYRAG 3 cut(s) 337, 455, 1289
Sse9I AATT 6 cut(s) 130, 210, 537, 708, 891, 930
SsiI CCGC 1 cut(s) 1265
SspMI CTAG 4 cut(s) 660, 998, 1137, 1372
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 5 cut(s) 89, 230, 789, 871, 912
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 5 cut(s) 67, 178, 243, 1054, 1231
TaqI TCGA 4 cut(s) 403, 585, 724, 1077
TaqII GACCGA 1 cut(s) 239
TasI AATT 6 cut(s) 130, 210, 537, 708, 891, 930
TatI WGTACW 1 cut(s) 1101
TfiI GAWTC 5 cut(s) 155, 257, 678, 847, 994
Tru1I TTAA 5 cut(s) 78, 111, 227, 398, 618
Tru9I TTAA 5 cut(s) 78, 111, 227, 398, 618
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 5 cut(s) 106, 262, 323, 608, 811
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 7 cut(s) 87, 129, 222, 1013, 1199, 1284, 1323
TspGWI ACGGA 1 cut(s) 1140
XapI RAATTY 1 cut(s) 537
XbaI TCTAGA 1 cut(s) 997
XceI RCATGY 1 cut(s) 615
XmnI GAANNNNTTC 2 cut(s) 464, 537
XspI CTAG 4 cut(s) 660, 998, 1137, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.