Rw3G026740

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005059
Physical Location & Seq
Reverse (-)
36895418 .. 36906963
11546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026740.1

Sequence Viewer

Length: 1404 bp
ATGGAGGAGAGAGCAAGATGCTTCACATTTCTCGGCGTCGGAGCTCTACTGGGCTCCGTCTCCACCTTTCTTCTGCTCAAGCTTCTACCAAGAAGGATTGCAAACCAATGTGATAAAAAGGCACTCCAAATGAATGATATTAATGGTAAAGGGTTGGCATCTGAGACACCTACAAATTGCAGTGCAGTTACTGGGAGCGGAAATAGCAAGTTAGCTGGTGTAGACCTTCTAAACGATGAGATAGTCTGTGAACAACTAACAAGAAACATTCAATTCTTTGGACTTGAACCTCAACAAAAAGTGACTGCTTCCTATGTTGTGGTCATTGGTCTTGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGGTCGGGGGTTGGCAGGCTTCTCCTTGTGGACTTTGATCAGGTATCAGTTTCATCACTTAATCGACATGCTGTTGCAACAAGAGCTGACGTTGGTATCCCAAAAGCCCAGTGTCTAAAAGATCATTTCTTGTCTATCTTCCCAGAGTGTCACATAGAAGCAAAGGTCCTGTTATACAATGCATCGTCTGAGGAAGAGATTCTTTCGGGTCATCCTGATTTTGTTTTGGACTGCATTGACAACATTGATACAAAGGTGGCACTTCTTGCGGCATGTGTACGTAGGGGTTTAAAGGTCCTATCAGCCACAGGAGCTGGTGCTAGAGCTGACCCGACAAGAATACGCATTGCTGATTTAAGACAATCGACAAATGACCCATTATCTCGAGCTGTGAGACACCGTTTGAAGAGAGAGTATGGCATTGAAGGTGGCATTCCTGTTGTATTTTCATTGGAGAAACCTAAGGCAAAGCTTCTTCCATTTAAAGGACAAAGTGGAGAAGAAGAAAATCCTTCAGATTACCAGATAGTACCAGGGTTTAGGGTTCGCATAATACCTGTCCTTGGTACCATTCCTGCAATATTTGGCCAAGTGATGGCCTCCTATGTGTTGACACAACTTGCAGAATTGCAAGTTCAATTGGAACCTGTGATAAACTTGGACATTGACCATTACCGGATGCTTCACCAACGTCTTATTGAGCATGAGGAGTCATTGTATGGCACTGCCTTGCATGTCCTGGTAGATGTTGAAGAAGTGATGTATGTTGCAAAAGAGTTGTGGCATGGAAGAAGTGCTAGAGAGCCATTTGCTAAAGATGTAGGACGTAGCATGTGGCGATCGGTGAATGAATTAATGCTTTTGAGGTGGGACTGGGAAAAGCCGGCATCTGTGTCCAACTTAATTCTCTTGAAATTTAAAGAGGCAGATGAACATGAATCCAGGACTCTTGAAGATATAAAAGAGGAGGAACCAGAATTTTTTGTTCGAGTTACATCTGTTCTGAAGCGAGCCGAAGTAGAATTTGGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

467

Amino Acids

51.85

Weight (kDa)

6.01

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 87 - 342 2.5e-44 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 935
AccB7I CCANNNNNTGG 1 cut(s) 964
AccBSI CCGCTC 1 cut(s) 198
AccI GTMKAC 1 cut(s) 222
AciI CCGC 3 cut(s) 198, 370, 638
AcoI YGGCCR 1 cut(s) 955
AcsI RAATTY 3 cut(s) 1283, 1346, 1391
AcuI CTGAAG 2 cut(s) 867, 1394
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 648, 900, 937
AfiI CCNNNNNNNGG 3 cut(s) 854, 932, 964
AgsI TTSAA 8 cut(s) 272, 287, 775, 794, 1007, 1121, 1282, 1322
AjnI CCWGG 3 cut(s) 901, 1107, 1310
AjuI GAANNNNNNNTTGG 1 cut(s) 1377
AluBI AGCT 8 cut(s) 44, 82, 215, 455, 683, 695, 758, 841
AluI AGCT 8 cut(s) 44, 82, 215, 455, 683, 695, 758, 841
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 3 cut(s) 64, 158, 757
AlwNI CAGNNNCTG 2 cut(s) 191, 683
Ama87I CYCGRG 1 cut(s) 753
AoxI GGCC 2 cut(s) 955, 966
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 3 cut(s) 1283, 1346, 1391
AseI ATTAAT 2 cut(s) 141, 1223
Asp700I GAANNNNTTC 1 cut(s) 567
Asp718I GGTACC 1 cut(s) 935
AspS9I GGNCC 2 cut(s) 535, 664
AsuHPI GGTGA 2 cut(s) 1046, 1225
AvaI CYCGRG 1 cut(s) 753
AvaII GGWCC 2 cut(s) 535, 664
AxyI CCTNAGG 1 cut(s) 831
BaeI ACNNNNGTAYC 4 cut(s) 448, 481, 609, 642
BalI TGGCCA 1 cut(s) 957
BanI GGYRCC 1 cut(s) 935
BanII GRGCYC 2 cut(s) 46, 56
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 340
BccI CCATC 1 cut(s) 958
BciT130I CCWGG 3 cut(s) 903, 1109, 1312
BciVI GTATCC 1 cut(s) 476
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 64, 158, 757
BfaI CTAG 2 cut(s) 690, 1167
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 2 cut(s) 354, 639
BlsI GCNGC 2 cut(s) 355, 640
Bme1390I CCNGG 3 cut(s) 903, 1109, 1312
Bme18I GGWCC 2 cut(s) 535, 664
BmeT110I CYCGRG 1 cut(s) 753
BmgT120I GGNCC 2 cut(s) 535, 664
BmiI GGNNCC 4 cut(s) 55, 937, 1014, 1341
BmrFI CCNGG 3 cut(s) 903, 1109, 1312
BmrI ACTGGG 4 cut(s) 59, 201, 472, 1252
BmsI GCATC 6 cut(s) 8, 167, 365, 560, 1038, 1265
BmuI ACTGGG 4 cut(s) 59, 201, 472, 1252
BpuEI CTTGAG 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 902, 931
BsaWI WCCGGW 1 cut(s) 1044
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 3 cut(s) 854, 932, 964
Bse118I RCCGGY 1 cut(s) 1252
Bse1I ACTGG 4 cut(s) 54, 196, 478, 1247
Bse21I CCTNAGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 714
BseBI CCWGG 3 cut(s) 903, 1109, 1312
BseDI CCNNGG 2 cut(s) 902, 931
BseGI GGATG 2 cut(s) 580, 1053
BseLI CCNNNNNNNGG 3 cut(s) 854, 932, 964
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 2 cut(s) 153, 549
BseNI ACTGG 4 cut(s) 54, 196, 478, 1247
BseRI GAGGAG 4 cut(s) 20, 351, 1091, 1349
BseXI GCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 204
Bsh1285I CGRYCG 2 cut(s) 374, 1211
BshFI GGCC 2 cut(s) 957, 968
BshNI GGYRCC 1 cut(s) 935
BsiEI CGRYCG 2 cut(s) 374, 1211
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 753
BsiSI CCGG 2 cut(s) 1045, 1253
BslFI GGGAC 1 cut(s) 1253
BslI CCNNNNNNNGG 3 cut(s) 854, 932, 964
BsmAI GTCTC 3 cut(s) 64, 158, 757
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 1253
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 2 cut(s) 957, 968
BsoBI CYCGRG 1 cut(s) 753
Bsp1286I GDGCHC 2 cut(s) 46, 56
Bsp143I GATC 3 cut(s) 406, 490, 1208
BspACI CCGC 3 cut(s) 198, 370, 638
BspANI GGCC 2 cut(s) 957, 968
BspCNI CTCAG 2 cut(s) 154, 550
BspLI GGNNCC 4 cut(s) 55, 937, 1014, 1341
BspT107I GGYRCC 1 cut(s) 935
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 1252
BsrI ACTGG 4 cut(s) 54, 196, 478, 1247
BssAI RCCGGY 1 cut(s) 1252
BssECI CCNNGG 2 cut(s) 902, 931
BssMI GATC 3 cut(s) 406, 490, 1208
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 931
Bst2UI CCWGG 3 cut(s) 903, 1109, 1312
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 2 cut(s) 558, 770
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBAI YACGTR 1 cut(s) 650
BstC8I GCNNGC 3 cut(s) 386, 1254, 1380
BstDEI CTNAG 3 cut(s) 162, 558, 831
BstF5I GGATG 2 cut(s) 580, 1053
BstKTI GATC 3 cut(s) 409, 493, 1211
BstMAI GTCTC 3 cut(s) 64, 158, 757
BstMBI GATC 3 cut(s) 406, 490, 1208
BstMCI CGRYCG 2 cut(s) 374, 1211
BstMWI GCNNNNNNNGC 4 cut(s) 204, 452, 635, 680
BstNI CCWGG 3 cut(s) 903, 1109, 1312
BstNSI RCATGY 4 cut(s) 440, 645, 1106, 1204
BstSCI CCNGG 3 cut(s) 901, 1107, 1310
BstSNI TACGTA 1 cut(s) 650
BstV1I GCAGC 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 831
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 2 cut(s) 957, 968
BtsCI GGATG 2 cut(s) 580, 1053
BtsI GCAGTG 2 cut(s) 187, 1092
BtsIMutI CAGTG 3 cut(s) 187, 485, 1092
Cac8I GCNNGC 3 cut(s) 386, 1254, 1380
CaiI CAGNNNCTG 2 cut(s) 191, 683
Cfr10I RCCGGY 1 cut(s) 1252
Cfr13I GGNCC 2 cut(s) 535, 664
CseI GACGC 1 cut(s) 25
Csp6I GTAC 3 cut(s) 647, 899, 936
CviAII CATG 8 cut(s) 350, 437, 642, 1073, 1103, 1154, 1201, 1304
CviQI GTAC 3 cut(s) 647, 899, 936
DdeI CTNAG 3 cut(s) 162, 558, 831
DpnI GATC 3 cut(s) 408, 492, 1210
DpnII GATC 3 cut(s) 406, 490, 1208
DraI TTTAAA 3 cut(s) 660, 853, 1288
EaeI YGGCCR 1 cut(s) 955
Eam1104I CTCTTC 2 cut(s) 558, 770
EarI CTCTTC 2 cut(s) 558, 770
Ecl136II GAGCTC 1 cut(s) 44
Eco105I TACGTA 1 cut(s) 650
Eco130I CCWWGG 1 cut(s) 931
Eco24I GRGCYC 2 cut(s) 46, 56
Eco47I GGWCC 2 cut(s) 535, 664
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 867, 1394
Eco81I CCTNAGG 1 cut(s) 831
Eco88I CYCGRG 1 cut(s) 753
EcoICRI GAGCTC 1 cut(s) 44
EcoO109I RGGNCCY 2 cut(s) 535, 664
EcoRII CCWGG 3 cut(s) 901, 1107, 1310
EcoT14I CCWWGG 1 cut(s) 931
EcoT22I ATGCAT 1 cut(s) 553
EcoT38I GRGCYC 2 cut(s) 46, 56
ErhI CCWWGG 1 cut(s) 931
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 8 cut(s) 353, 440, 645, 1076, 1106, 1157, 1204, 1307
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FaqI GGGAC 1 cut(s) 1253
FatI CATG 8 cut(s) 349, 436, 641, 1072, 1102, 1153, 1200, 1303
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 2 cut(s) 354, 639
FokI GGATG 2 cut(s) 567, 1060
FriOI GRGCYC 2 cut(s) 46, 56
Fsp4HI GCNGC 2 cut(s) 354, 639
FspBI CTAG 2 cut(s) 690, 1167
GluI GCNGC 2 cut(s) 354, 639
HaeIII GGCC 2 cut(s) 957, 968
HapII CCGG 2 cut(s) 1045, 1253
HgaI GACGC 1 cut(s) 25
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 8 cut(s) 353, 440, 645, 1076, 1106, 1157, 1204, 1307
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 2 cut(s) 80, 839
HinfI GANTC 4 cut(s) 568, 1079, 1307, 1315
HpaII CCGG 2 cut(s) 1045, 1253
HphI GGTGA 2 cut(s) 1046, 1225
Hpy166II GTNNAC 5 cut(s) 223, 251, 400, 647, 981
Hpy188I TCNGA 5 cut(s) 41, 163, 559, 886, 1374
Hpy188III TCNNGA 4 cut(s) 584, 753, 1279, 1319
Hpy8I GTNNAC 5 cut(s) 223, 251, 400, 647, 981
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 87, 236, 788, 891
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4IV ACGT 4 cut(s) 459, 649, 1060, 1195
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 452, 635, 680
HpyF3I CTNAG 3 cut(s) 162, 558, 831
HpySE526I ACGT 4 cut(s) 459, 649, 1060, 1195
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 8 cut(s) 353, 440, 645, 1076, 1106, 1157, 1204, 1307
KpnI GGTACC 1 cut(s) 939
KroI GCCGGC 1 cut(s) 1252
KroNI GCCGGC 1 cut(s) 1254
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 3 cut(s) 406, 490, 1208
LmnI GCTCC 4 cut(s) 41, 59, 195, 680
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 6 cut(s) 8, 167, 365, 560, 1038, 1265
MaeI CTAG 2 cut(s) 690, 1167
MaeII ACGT 4 cut(s) 459, 649, 1060, 1195
MaeIII GTNAC 4 cut(s) 187, 301, 518, 1360
MalI GATC 3 cut(s) 408, 492, 1210
MbiI CCGCTC 1 cut(s) 198
MboI GATC 3 cut(s) 406, 490, 1208
MfeI CAATTG 1 cut(s) 1007
MhlI GDGCHC 2 cut(s) 46, 56
MlsI TGGCCA 1 cut(s) 957
MluCI AATT 9 cut(s) 175, 272, 995, 1007, 1220, 1272, 1283, 1346, 1391
MluNI TGGCCA 1 cut(s) 957
MlyI GAGTC 2 cut(s) 1088, 1309
MmeI TCCRAC 3 cut(s) 19, 322, 1290
MnlI CCTC 9 cut(s) 300, 329, 553, 979, 1069, 1227, 1285, 1327, 1330
Mox20I TGGCCA 1 cut(s) 957
Mph1103I ATGCAT 1 cut(s) 553
MroNI GCCGGC 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 1 cut(s) 957
MseI TTAA 8 cut(s) 141, 429, 659, 725, 852, 1223, 1271, 1287
Msp20I TGGCCA 1 cut(s) 957
MspI CCGG 2 cut(s) 1045, 1253
MspR9I CCNGG 3 cut(s) 903, 1109, 1312
MunI CAATTG 1 cut(s) 1007
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 3 cut(s) 903, 1109, 1312
MwoI GCNNNNNNNGC 4 cut(s) 204, 452, 635, 680
NaeI GCCGGC 1 cut(s) 1254
NdeII GATC 3 cut(s) 406, 490, 1208
NgoMIV GCCGGC 1 cut(s) 1252
NlaIII CATG 8 cut(s) 353, 440, 645, 1076, 1106, 1157, 1204, 1307
NlaIV GGNNCC 4 cut(s) 55, 937, 1014, 1341
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 2 cut(s) 301, 518
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 4 cut(s) 440, 645, 1106, 1204
PaeR7I CTCGAG 1 cut(s) 753
PctI GAATGC 1 cut(s) 801
PdiI GCCGGC 1 cut(s) 1254
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 2 cut(s) 568, 1307
PflMI CCANNNNNTGG 1 cut(s) 964
PfoI TCCNGGA 1 cut(s) 1310
PkrI GCNGC 2 cut(s) 355, 640
Ple19I CGATCG 1 cut(s) 1211
PleI GAGTC 2 cut(s) 1087, 1309
PpsI GAGTC 2 cut(s) 1087, 1309
Ppu21I YACGTR 1 cut(s) 650
PpuMI RGGWCCY 2 cut(s) 535, 664
PshBI ATTAAT 2 cut(s) 141, 1223
Psp124BI GAGCTC 1 cut(s) 46
Psp5II RGGWCCY 2 cut(s) 535, 664
Psp6I CCWGG 3 cut(s) 901, 1107, 1310
PspGI CCWGG 3 cut(s) 901, 1107, 1310
PspN4I GGNNCC 4 cut(s) 55, 937, 1014, 1341
PspPI GGNCC 2 cut(s) 535, 664
PspPPI RGGWCCY 2 cut(s) 535, 664
PstNI CAGNNNCTG 2 cut(s) 191, 683
PvuI CGATCG 1 cut(s) 1211
RsaI GTAC 3 cut(s) 648, 900, 937
RsaNI GTAC 3 cut(s) 647, 899, 936
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 8 cut(s) 141, 429, 659, 725, 852, 1223, 1271, 1287
SatI GCNGC 2 cut(s) 354, 639
Sau3AI GATC 3 cut(s) 406, 490, 1208
Sau96I GGNCC 2 cut(s) 535, 664
SchI GAGTC 2 cut(s) 1088, 1309
ScrFI CCNGG 3 cut(s) 903, 1109, 1312
SduI GDGCHC 2 cut(s) 46, 56
SfaNI GCATC 6 cut(s) 8, 167, 365, 560, 1038, 1265
Sfr274I CTCGAG 1 cut(s) 753
SinI GGWCC 2 cut(s) 535, 664
SlaI CTCGAG 1 cut(s) 753
SmlI CTYRAG 2 cut(s) 77, 753
SmoI CTYRAG 2 cut(s) 77, 753
SnaBI TACGTA 1 cut(s) 650
Sse9I AATT 9 cut(s) 175, 272, 995, 1007, 1220, 1272, 1283, 1346, 1391
SsiI CCGC 3 cut(s) 198, 370, 638
SspI AATATT 1 cut(s) 951
SspMI CTAG 2 cut(s) 690, 1167
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 3 cut(s) 901, 1107, 1310
StyI CCWWGG 1 cut(s) 931
TaaI ACNGT 1 cut(s) 770
TaiI ACGT 4 cut(s) 462, 652, 1063, 1198
TaqI TCGA 4 cut(s) 433, 734, 754, 1357
TasI AATT 9 cut(s) 175, 272, 995, 1007, 1220, 1272, 1283, 1346, 1391
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 2 cut(s) 568, 1307
Tru1I TTAA 8 cut(s) 141, 429, 659, 725, 852, 1223, 1271, 1287
Tru9I TTAA 8 cut(s) 141, 429, 659, 725, 852, 1223, 1271, 1287
TscAI CASTG 3 cut(s) 187, 485, 1099
TseFI GTSAC 2 cut(s) 301, 518
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 2 cut(s) 301, 518
TspDTI ATGAA 6 cut(s) 146, 411, 807, 1233, 1314, 1320
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 3 cut(s) 187, 485, 1099
Van91I CCANNNNNTGG 1 cut(s) 964
VpaK11BI GGWCC 2 cut(s) 535, 664
VspI ATTAAT 2 cut(s) 141, 1223
XapI RAATTY 3 cut(s) 1283, 1346, 1391
XceI RCATGY 4 cut(s) 440, 645, 1106, 1204
XhoI CTCGAG 1 cut(s) 753
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 690, 1167
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.