MD09G1267400.v1.1

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005059
Physical Location & Seq
Forward (+)
34167177 .. 34174930
7754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1267400.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGGAGGAGAGAGCAAAATGCTTGGCATTGCTCGGATTCGGAGCTCTGGTAGGCTCTGTTTCCACCATCCTTCTCTTGAAGTTTCGACCAAGGACTGTCAACGGATGCGATGAAAAGGGAGGTATAGGGCCTGTATCCAAGACACGAACAAATTGTTGTGCAGTTTCTGGGAATGGGAATAACAAGATGGCTGGTGTAGATCTTCTAAGTGATGAAATAGTTTCTGAACAACTAACAAGGAACATTCAATTTTTCGGACTTGAGCCTCAACAAAAAGTTACTGCATCCTATGTTGTGGTCATTGGTCTTGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGATCAGGGGTTGGAAGGCTCCTCCTTGTGGACTTTGATCAGGTATCAGTTTCATCACTCAATCGACATGCTGTTGCAACAAGAGCAGATGTTGGCATCCCTAAGGCTCAATGTCTGAAAGAGCATTTCTTGTCAATCTTCCCAGAGTGCCACATAGATGCAAAAGTTTTATTATACAATGAATCATCTGAAGAAGAAATCCTTGCGGGTCATCCTGATTTTGTTTTAGATTGCATTGATAACATCGATACAAAGGTGGCACTTCTTGCTGCATGTGTGCGTAGGGGTTTGAATGTATTATCAGCAACAGGAGCTGGTGCTAGAGCTGACCCAACTAGAATACGCGTTGCTGATTTAAGACAATCAACAAATGATCCATTATCCCGATCTGTAAGACACCGTTTAAGGAGAGATTATGGTATTGAGGGTGGCGTAACTGTTGTATTTTCATTAGAGAAGCCTAAGGCAAAGCTTCTTCCATTCAAAGGACCAAGTGGAGAGGAAGAAAATCCTTCAGATTATCAGATCCTACCGGGGTTCAGGGTTCGCATAATACCTGTTCTTGGCACCATCCCTGCAATATTTGGACAGGTCATGGCCTCCTTTGTTTTGACGCAACTGGCTGGATTGCAAGTTCAAACAGAGCCTATAGTAAATTTGGACATTGACCATTACCGGATGCTTCACCAACGCCTTATTGAACATGAGGAGTCATTGTATGGAACTGCCTTGCATGTCCAGGTGGATGTTGAGGAAGTGATGTATATTGCAAAAGAGTTGTGGCATGGAAGAAGTGCAAGAGAGCCATTCGCTAAAGATGTAGGACGTGGGATGTGGCGATCGGTAAATGAGTTAATGCTTGTGAGGTGGGACAGGGAAAAGCCGGCATCTGTATCCAACCTAATTCTCTTAAAATTTAAAGAGGCAGATGAACATGAATCGATTACTCTGGAGGATATTAAAGAAAGAGAAGCAGAGTTTTTCGATAGAGTGACCTCTGTTCTGAAGCGTGCTGAGCTAGAATTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

460

Amino Acids

50.69

Weight (kDa)

6.11

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 79 - 340 3.5e-44 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 911
AccII CGCG 1 cut(s) 690
AciI CCGC 1 cut(s) 551
AclWI GGATC 2 cut(s) 713, 865
AcsI RAATTY 3 cut(s) 1000, 1259, 1367
AcuI CTGAAG 3 cut(s) 555, 843, 1370
AfiI CCNNNNNNNGG 3 cut(s) 373, 830, 908
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 6 cut(s) 79, 248, 637, 829, 983, 1046
AjiI CACGTC 1 cut(s) 1172
AjnI CCWGG 1 cut(s) 1083
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 5 cut(s) 44, 659, 671, 817, 1363
AluI AGCT 5 cut(s) 44, 659, 671, 817, 1363
Alw21I GWGCWC 1 cut(s) 46
AlwI GGATC 2 cut(s) 713, 865
AlwNI CAGNNNCTG 2 cut(s) 167, 659
AoxI GGCC 2 cut(s) 128, 942
ApeKI GCWGC 2 cut(s) 329, 614
ApoI RAATTY 3 cut(s) 1000, 1259, 1367
AspS9I GGNCC 2 cut(s) 128, 833
AsuC2I CCSGG 1 cut(s) 879
AsuHPI GGTGA 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 833
AxyI CCTNAGG 2 cut(s) 447, 807
BaeI ACNNNNGTAYC 2 cut(s) 585, 618
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 316, 601
BccI CCATC 3 cut(s) 74, 181, 923
BciT130I CCWGG 1 cut(s) 1085
BciVI GTATCC 2 cut(s) 145, 1249
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 879
BfaI CTAG 3 cut(s) 666, 681, 1364
BfmI CTRYAG 1 cut(s) 993
BfuI GTATCC 2 cut(s) 145, 1249
BglII AGATCT 1 cut(s) 199
BisI GCNGC 2 cut(s) 330, 615
BlpI GCTNAGC 1 cut(s) 1359
BlsI GCNGC 2 cut(s) 331, 616
Bme1390I CCNGG 2 cut(s) 879, 1085
Bme18I GGWCC 1 cut(s) 833
BmgBI CACGTC 1 cut(s) 1172
BmgT120I GGNCC 2 cut(s) 128, 833
BmiI GGNNCC 2 cut(s) 365, 913
BmrFI CCNGG 2 cut(s) 879, 1085
BmsI GCATC 7 cut(s) 95, 293, 341, 450, 493, 1014, 1241
BpmI CTGGAG 1 cut(s) 1316
Bpu1102I GCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 879
Bsa29I ATCGAT 2 cut(s) 591, 1286
BsaJI CCNNGG 2 cut(s) 89, 878
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 1046, 1076
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 830, 908
Bse118I RCCGGY 1 cut(s) 1228
Bse1I ACTGG 1 cut(s) 969
Bse21I CCTNAGG 2 cut(s) 447, 807
Bse3DI GCAATG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 1085
BseCI ATCGAT 2 cut(s) 591, 1286
BseDI CCNNGG 2 cut(s) 89, 878
BseGI GGATG 9 cut(s) 66, 110, 284, 441, 556, 915, 1029, 1096, 1182
BseLI CCNNNNNNNGG 3 cut(s) 373, 830, 908
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 1350
BseNI ACTGG 1 cut(s) 969
BseRI GAGGAG 4 cut(s) 20, 327, 356, 1067
BseXI GCAGC 2 cut(s) 316, 601
BsgI GTGCAG 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 690
Bsh1285I CGRYCG 1 cut(s) 1187
BshFI GGCC 2 cut(s) 130, 944
BshNI GGYRCC 1 cut(s) 911
BshVI ATCGAT 2 cut(s) 591, 1286
BsiEI CGRYCG 1 cut(s) 1187
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 3 cut(s) 878, 1021, 1229
BslFI GGGAC 1 cut(s) 1229
BslI CCNNNNNNNGG 3 cut(s) 373, 830, 908
BsmFI GGGAC 1 cut(s) 1229
BsnI GGCC 2 cut(s) 130, 944
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
Bsp1720I GCTNAGC 1 cut(s) 1359
BspACI CCGC 1 cut(s) 551
BspANI GGCC 2 cut(s) 130, 944
BspCNI CTCAG 1 cut(s) 1351
BspDI ATCGAT 2 cut(s) 591, 1286
BspFNI CGCG 1 cut(s) 690
BspLI GGNNCC 2 cut(s) 365, 913
BspPI GGATC 2 cut(s) 713, 865
BspT107I GGYRCC 1 cut(s) 911
BsrDI GCAATG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 1228
BsrI ACTGG 1 cut(s) 969
BssAI RCCGGY 1 cut(s) 1228
BssECI CCNNGG 2 cut(s) 89, 878
BssMI GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 1085
Bst4CI ACNGT 3 cut(s) 97, 746, 784
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 2 cut(s) 1230, 1356
BstDEI CTNAG 4 cut(s) 206, 447, 807, 1359
BstF5I GGATG 9 cut(s) 66, 110, 284, 441, 556, 915, 1029, 1096, 1182
BstFNI CGCG 1 cut(s) 690
BstKTI GATC 7 cut(s) 202, 350, 385, 721, 734, 873, 1187
BstMBI GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
BstMCI CGRYCG 1 cut(s) 1187
BstMWI GCNNNNNNNGC 4 cut(s) 428, 611, 656, 1360
BstNI CCWGG 1 cut(s) 1085
BstNSI RCATGY 3 cut(s) 416, 621, 1082
BstSCI CCNGG 2 cut(s) 877, 1083
BstSFI CTRYAG 1 cut(s) 993
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 2 cut(s) 316, 601
BstX2I RGATCY 2 cut(s) 199, 870
BstYI RGATCY 2 cut(s) 199, 870
Bsu15I ATCGAT 2 cut(s) 591, 1286
Bsu36I CCTNAGG 2 cut(s) 447, 807
BsuI GTATCC 2 cut(s) 145, 1249
BsuRI GGCC 2 cut(s) 130, 944
BsuTUI ATCGAT 2 cut(s) 591, 1286
BtgZI GCGATG 1 cut(s) 123
BtrI CACGTC 1 cut(s) 1172
BtsCI GGATG 9 cut(s) 66, 110, 284, 441, 556, 915, 1029, 1096, 1182
Cac8I GCNNGC 2 cut(s) 1230, 1356
CaiI CAGNNNCTG 2 cut(s) 167, 659
Cfr10I RCCGGY 1 cut(s) 1228
Cfr13I GGNCC 2 cut(s) 128, 833
ClaI ATCGAT 2 cut(s) 591, 1286
CseI GACGC 1 cut(s) 967
CviAII CATG 8 cut(s) 326, 413, 618, 940, 1049, 1079, 1130, 1280
DdeI CTNAG 4 cut(s) 206, 447, 807, 1359
DpnI GATC 7 cut(s) 201, 349, 384, 720, 733, 872, 1186
DpnII GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
DraI TTTAAA 1 cut(s) 1264
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 833
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 3 cut(s) 555, 843, 1370
Eco81I CCTNAGG 2 cut(s) 447, 807
EcoICRI GAGCTC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 1083
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 8 cut(s) 329, 416, 621, 943, 1052, 1082, 1133, 1283
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 1 cut(s) 1229
FatI CATG 8 cut(s) 325, 412, 617, 939, 1048, 1078, 1129, 1279
FauI CCCGC 1 cut(s) 544
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 330, 615
FokI GGATG 9 cut(s) 53, 117, 271, 428, 543, 902, 1036, 1103, 1189
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 2 cut(s) 330, 615
FspBI CTAG 3 cut(s) 666, 681, 1364
GluI GCNGC 2 cut(s) 330, 615
GsuI CTGGAG 1 cut(s) 1316
HaeIII GGCC 2 cut(s) 130, 944
HapII CCGG 3 cut(s) 878, 1021, 1229
HgaI GACGC 1 cut(s) 967
Hin1II CATG 8 cut(s) 329, 416, 621, 943, 1052, 1082, 1133, 1283
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 4 cut(s) 36, 527, 1055, 1283
HpaII CCGG 3 cut(s) 878, 1021, 1229
HphI GGTGA 1 cut(s) 1022
Hpy166II GTNNAC 2 cut(s) 100, 376
Hpy188I TCNGA 9 cut(s) 35, 41, 226, 257, 462, 535, 862, 870, 1350
Hpy188III TCNNGA 4 cut(s) 76, 560, 729, 1295
Hpy8I GTNNAC 2 cut(s) 100, 376
HpyAV CCTTC 3 cut(s) 80, 354, 867
HpyCH4III ACNGT 3 cut(s) 97, 746, 784
HpyCH4IV ACGT 1 cut(s) 1171
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 611, 656, 1360
HpyF3I CTNAG 4 cut(s) 206, 447, 807, 1359
HpySE526I ACGT 1 cut(s) 1171
Hsp92II CATG 8 cut(s) 329, 416, 621, 943, 1052, 1082, 1133, 1283
KroI GCCGGC 1 cut(s) 1228
KroNI GCCGGC 1 cut(s) 1230
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
LmnI GCTCC 3 cut(s) 41, 369, 656
Lsp1109I GCAGC 2 cut(s) 316, 601
LweI GCATC 7 cut(s) 95, 293, 341, 450, 493, 1014, 1241
MaeI CTAG 3 cut(s) 666, 681, 1364
MaeII ACGT 1 cut(s) 1171
MaeIII GTNAC 3 cut(s) 277, 778, 1336
MalI GATC 7 cut(s) 201, 349, 384, 720, 733, 872, 1186
MboI GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
MboII GAAGA 7 cut(s) 194, 475, 548, 551, 812, 860, 1146
MflI RGATCY 2 cut(s) 199, 870
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 6 cut(s) 151, 248, 1000, 1248, 1259, 1367
MluI ACGCGT 1 cut(s) 688
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 3 cut(s) 298, 337, 1266
MroNI GCCGGC 1 cut(s) 1228
MseI TTAA 6 cut(s) 701, 749, 1199, 1256, 1263, 1305
MslI CAYNNNNRTG 1 cut(s) 501
MspI CCGG 3 cut(s) 878, 1021, 1229
MspR9I CCNGG 2 cut(s) 879, 1085
MvaI CCWGG 1 cut(s) 1085
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 4 cut(s) 428, 611, 656, 1360
NaeI GCCGGC 1 cut(s) 1230
NciI CCSGG 1 cut(s) 879
NdeII GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
NgoMIV GCCGGC 1 cut(s) 1228
NlaIII CATG 8 cut(s) 329, 416, 621, 943, 1052, 1082, 1133, 1283
NlaIV GGNNCC 2 cut(s) 365, 913
NmuCI GTSAC 1 cut(s) 1336
NspI RCATGY 3 cut(s) 416, 621, 1082
PdiI GCCGGC 1 cut(s) 1230
PfeI GAWTC 3 cut(s) 36, 527, 1283
PkrI GCNGC 2 cut(s) 331, 616
Ple19I CGATCG 1 cut(s) 1187
PleI GAGTC 1 cut(s) 1063
PpsI GAGTC 1 cut(s) 1063
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 1083
PspGI CCWGG 1 cut(s) 1083
PspN4I GGNNCC 2 cut(s) 365, 913
PspPI GGNCC 2 cut(s) 128, 833
PstNI CAGNNNCTG 2 cut(s) 167, 659
PsuI RGATCY 2 cut(s) 199, 870
PvuI CGATCG 1 cut(s) 1187
RseI CAYNNNNRTG 1 cut(s) 501
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 6 cut(s) 701, 749, 1199, 1256, 1263, 1305
SatI GCNGC 2 cut(s) 330, 615
Sau3AI GATC 7 cut(s) 199, 347, 382, 718, 731, 870, 1184
Sau96I GGNCC 2 cut(s) 128, 833
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 2 cut(s) 879, 1085
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 7 cut(s) 95, 293, 341, 450, 493, 1014, 1241
SfcI CTRYAG 1 cut(s) 993
SinI GGWCC 1 cut(s) 833
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 6 cut(s) 151, 248, 1000, 1248, 1259, 1367
SsiI CCGC 1 cut(s) 551
SspI AATATT 1 cut(s) 927
SspMI CTAG 3 cut(s) 666, 681, 1364
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 2 cut(s) 877, 1083
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 3 cut(s) 97, 746, 784
TaiI ACGT 1 cut(s) 1174
TaqI TCGA 5 cut(s) 85, 409, 591, 1286, 1329
TasI AATT 6 cut(s) 151, 248, 1000, 1248, 1259, 1367
TfiI GAWTC 3 cut(s) 36, 527, 1283
Tru1I TTAA 6 cut(s) 701, 749, 1199, 1256, 1263, 1305
Tru9I TTAA 6 cut(s) 701, 749, 1199, 1256, 1263, 1305
TseFI GTSAC 1 cut(s) 1336
TseI GCWGC 2 cut(s) 329, 614
Tsp45I GTSAC 1 cut(s) 1336
TspDTI ATGAA 7 cut(s) 126, 228, 387, 540, 783, 1290, 1296
TspGWI ACGGA 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 833
XapI RAATTY 3 cut(s) 1000, 1259, 1367
XceI RCATGY 3 cut(s) 416, 621, 1082
XspI CTAG 3 cut(s) 666, 681, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.