Rorug03G0253100

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005059
Physical Location & Seq
Forward (+)
24004328 .. 24005036
709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0253100.1

Sequence Viewer

Length: 591 bp
ATGGACTTGAGGCGCCATTTCAAGCGTGTTTGGGTACTGAACCAAGGATTTACCGTGCAGCAAAGAGCCCCAGATCGATTCTTGTTCTCGTTCGACCTCCGCCGTGATAGCCGGAAAGTGATGATGGGAGGACCGTGGAGTTTTAATCGGGCTCTGGTCGTGATGCAGCCTTATGATGGTATTGCTCCTCTGCCATCGGTACAAATGCACGACCTATTCTTTTGGGTCCGCATAACCAATATGCCACCGGCATATGAACAACGGAAAACTATTGCTAATGTAGCCTCGGTTGCCGGGATTGAGCGAGAGGTACACTTTCGTTTTGAAAATCTAGTAGGCAAGTGTGATATATGTGCTCGTATCTTCCATGAGAATGGTCCATGCAATCCGGATACGTTGCCAAATGGTATCAAGCCTGTTGGACAACCTGTAGCTCGTAAGCTCGAACTCCCTCAGCCGTTTCTGGGATTCCATGGTCCTCAGTTCAAGGAGGGTGCATTCCGGTTTAAAGGCATCACTACTCCCATTCTGCCCTCGGTGGGGCGTTCCTTGTTTGGTGGTGCTGGGTCTAGCAAGCCTCTAGGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

196

Amino Acids

22.16

Weight (kDa)

10.22

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 2 - 59 1.2e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 2 cut(s) 100, 229
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 3 cut(s) 36, 201, 312
AfiI CCNNNNNNNGG 4 cut(s) 176, 464, 539, 540
AgsI TTSAA 3 cut(s) 22, 326, 487
AluBI AGCT 2 cut(s) 434, 442
AluI AGCT 2 cut(s) 434, 442
Alw21I GWGCWC 1 cut(s) 358
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 2 cut(s) 58, 166
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 4 cut(s) 131, 226, 377, 476
AsuC2I CCSGG 1 cut(s) 295
AvaII GGWCC 4 cut(s) 131, 226, 377, 476
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 2 cut(s) 70, 154
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 70, 178
BccI CCATC 3 cut(s) 118, 170, 202
BceAI ACGGC 2 cut(s) 87, 442
BciVI GTATCC 1 cut(s) 385
BcnI CCSGG 1 cut(s) 295
BfaI CTAG 3 cut(s) 332, 570, 581
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 16
BfuI GTATCC 1 cut(s) 385
BisI GCNGC 2 cut(s) 59, 167
BlsI GCNGC 2 cut(s) 60, 168
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 4 cut(s) 131, 226, 377, 476
BmgT120I GGNCC 4 cut(s) 131, 226, 377, 476
BmiI GGNNCC 2 cut(s) 14, 227
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 2 cut(s) 153, 522
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 295
Bsa29I ATCGAT 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 13
BsaJI CCNNGG 5 cut(s) 43, 134, 285, 472, 534
BsaWI WCCGGW 2 cut(s) 388, 501
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 464, 539, 540
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 388
BseCI ATCGAT 1 cut(s) 76
BseDI CCNNGG 5 cut(s) 43, 134, 285, 472, 534
BseLI CCNNNNNNNGG 4 cut(s) 176, 464, 539, 540
BseMII CTCAG 2 cut(s) 467, 494
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 70, 178
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 585
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 5 cut(s) 112, 248, 294, 389, 502
BslI CCNNNNNNNGG 4 cut(s) 176, 464, 539, 540
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 1 cut(s) 585
Bsp1286I GDGCHC 3 cut(s) 70, 154, 358
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 2 cut(s) 100, 229
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 2 cut(s) 466, 493
BspDI ATCGAT 1 cut(s) 76
BspEI TCCGGA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 14, 227
BspT107I GGYRCC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 1 cut(s) 586
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 5 cut(s) 43, 134, 285, 472, 534
BssMI GATC 1 cut(s) 73
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 43, 472
Bst4CI ACNGT 2 cut(s) 55, 135
BstACI GRCGYC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 2 cut(s) 453, 480
BstDSI CCRYGG 2 cut(s) 134, 472
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 3 cut(s) 108, 281, 290
BstSCI CCNGG 1 cut(s) 293
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 70, 178
BstXI CCANNNNNNTGG 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 76
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 1 cut(s) 585
BsuTUI ATCGAT 1 cut(s) 76
BtgI CCRYGG 2 cut(s) 134, 472
Cac8I GCNNGC 1 cut(s) 575
CfoI GCGC 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 4 cut(s) 131, 226, 377, 476
ClaI ATCGAT 1 cut(s) 76
Csp6I GTAC 3 cut(s) 35, 200, 311
CviAII CATG 3 cut(s) 368, 381, 473
CviQI GTAC 3 cut(s) 35, 200, 311
DdeI CTNAG 2 cut(s) 453, 480
DinI GGCGCC 1 cut(s) 14
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
DraI TTTAAA 1 cut(s) 508
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 43, 472
Eco24I GRGCYC 2 cut(s) 70, 154
Eco47I GGWCC 4 cut(s) 131, 226, 377, 476
EcoT14I CCWWGG 2 cut(s) 43, 472
EcoT38I GRGCYC 2 cut(s) 70, 154
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 2 cut(s) 43, 472
FaeI CATG 3 cut(s) 371, 384, 476
FatI CATG 3 cut(s) 367, 380, 472
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 59, 167
FriOI GRGCYC 2 cut(s) 70, 154
Fsp4HI GCNGC 2 cut(s) 59, 167
FspBI CTAG 3 cut(s) 332, 570, 581
GlaI GCGC 1 cut(s) 14
GluI GCNGC 2 cut(s) 59, 167
GsaI CCCAGC 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 1 cut(s) 585
HapII CCGG 5 cut(s) 112, 248, 294, 389, 502
HhaI GCGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 3 cut(s) 371, 384, 476
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HinfI GANTC 2 cut(s) 78, 468
HpaII CCGG 5 cut(s) 112, 248, 294, 389, 502
Hpy166II GTNNAC 1 cut(s) 313
Hpy188III TCNNGA 2 cut(s) 160, 389
Hpy8I GTNNAC 1 cut(s) 313
HpyCH4III ACNGT 2 cut(s) 55, 135
HpyCH4IV ACGT 1 cut(s) 395
HpyCH4V TGCA 5 cut(s) 58, 166, 208, 384, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 281, 290
HpyF3I CTNAG 2 cut(s) 453, 480
HpySE526I ACGT 1 cut(s) 395
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 3 cut(s) 371, 384, 476
HspAI GCGC 1 cut(s) 13
KasI GGCGCC 1 cut(s) 12
Kpn2I TCCGGA 1 cut(s) 388
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 2 cut(s) 70, 178
LweI GCATC 2 cut(s) 153, 522
MaeI CTAG 3 cut(s) 332, 570, 581
MaeII ACGT 1 cut(s) 395
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 1 cut(s) 355
MhlI GDGCHC 3 cut(s) 70, 154, 358
Mly113I GGCGCC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 400
MroI TCCGGA 1 cut(s) 388
MseI TTAA 2 cut(s) 144, 507
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 5 cut(s) 112, 248, 294, 389, 502
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 497
MwoI GCNNNNNNNGC 3 cut(s) 108, 281, 290
NarI GGCGCC 1 cut(s) 13
NciI CCSGG 1 cut(s) 295
NcoI CCATGG 1 cut(s) 472
NdeI CATATG 1 cut(s) 253
NdeII GATC 1 cut(s) 73
NlaIII CATG 3 cut(s) 371, 384, 476
NlaIV GGNNCC 2 cut(s) 14, 227
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 2 cut(s) 78, 468
PkrI GCNGC 2 cut(s) 60, 168
PluTI GGCGCC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 14, 227
PspPI GGNCC 4 cut(s) 131, 226, 377, 476
RsaI GTAC 3 cut(s) 36, 201, 312
RsaNI GTAC 3 cut(s) 35, 200, 311
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 2 cut(s) 144, 507
SatI GCNGC 2 cut(s) 59, 167
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 4 cut(s) 131, 226, 377, 476
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 3 cut(s) 70, 154, 358
SetI ASST 7 cut(s) 99, 216, 312, 398, 430, 436, 444
SfaNI GCATC 2 cut(s) 153, 522
SfcI CTRYAG 1 cut(s) 429
SfoI GGCGCC 1 cut(s) 14
SinI GGWCC 4 cut(s) 131, 226, 377, 476
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SsiI CCGC 2 cut(s) 100, 229
SspDI GGCGCC 1 cut(s) 12
SspMI CTAG 3 cut(s) 332, 570, 581
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 2 cut(s) 43, 472
TaaI ACNGT 2 cut(s) 55, 135
TaiI ACGT 1 cut(s) 398
TaqI TCGA 3 cut(s) 76, 93, 444
TfiI GAWTC 2 cut(s) 78, 468
Tru1I TTAA 2 cut(s) 144, 507
Tru9I TTAA 2 cut(s) 144, 507
TseI GCWGC 2 cut(s) 58, 166
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 1 cut(s) 277
VpaK11BI GGWCC 4 cut(s) 131, 226, 377, 476
XspI CTAG 3 cut(s) 332, 570, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.