pycom09g18020

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005059
Physical Location & Seq
Forward (+)
18628120 .. 18633405
5286 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g18020.1

Sequence Viewer

Length: 1017 bp
ATGTTGGACCCTGAGGCTACAAAAAAAATCTTAAAAATCATAAGTATAACAGCAAAATCTAAAGGCTCCGCCTCTGCTAGAGAGCTTCGGGTGCCACTGTCAACCCGGTTACCGGAGTTGCGATGCCTAAAACCCTGTCTGTCCCCCTTCCCCGCCAATGTTCCGGAGATTGGGGTGTTTGGCAACCCAGAAAAACGAAAGAAAATAAGAAAAAACCAGAGGTTGGAGGGAGGGCGGCAGAAGATGGAGGAGAGAGCAAGATGCTTGGCATTGCTCGGATTTGGAGCTCTGGTAGGCTCTGTTTCCACCATCCTTCTCTTGAAGTTTCGACCAAGGACTGTCAACGGATGCGATGAAAAGGGAGGTATACGGCCTGTTTCCAAGACACGAACAAATTGTTGTGCAGTTCCTGGGAATGGGAATAACAAGATGGCTGGTGTAGATCTTCTAAGTGATGAAATAGTTTCTGAACAACTAACAAGGAACATTCAATTTTTCGGACTTGAGCCTCAACAAAAAGTTACTGCATCCTATGTTGTGGTAATTGGTCTTGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGATCAGGGGTTGGAAGGCTCCTCCTTGTGGACTTTGATCAGGTATCAGTTTCATCCCTCAATCGACATGCTGTTGCAACAAGAGCAGATGTTGGCATCCCAAAAGCTCAATGTCTGAAAGAGCATTTCTTGTCAATCTTCCCAGAGTGCCACATAGATGCAAAAGTTTTATTATACAATGAATCATCTGAAGAAGAAATCCTAGCGGGTCATCCTGATTTTGTTTTAGATTGCATTGATAACATTGATACAAAGGTGGCACTTCTTGCTGCGTGTGTACGTAGGGGTTTGAATGTATTATCAGCAACAGGAGCTGGTGCTAGAGCTGACCCAACTAGAATACGCGTTGCTGATTTAAGACAATCAACAAATGATCCATTATCCCGATCTCTTTGTACGACACCGTTTAAGGAGAGATTATGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

339

Amino Acids

36.77

Weight (kDa)

9.43

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 160 - 311 1.3e-41 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 223
AccI GTMKAC 1 cut(s) 367
AccII CGCG 1 cut(s) 933
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 4 cut(s) 69, 153, 235, 794
AclWI GGATC 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 798
AfaI GTAC 2 cut(s) 867, 985
AfiI CCNNNNNNNGG 5 cut(s) 112, 170, 223, 416, 616
AflIII ACRYGT 1 cut(s) 931
AgsI TTSAA 3 cut(s) 322, 491, 880
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 5 cut(s) 85, 287, 695, 902, 914
AluI AGCT 5 cut(s) 85, 287, 695, 902, 914
Alw21I GWGCWC 1 cut(s) 289
AlwI GGATC 1 cut(s) 956
AlwNI CAGNNNCTG 2 cut(s) 410, 902
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 572, 857
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 106
AvaII GGWCC 1 cut(s) 7
AxyI CCTNAGG 1 cut(s) 12
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 2 cut(s) 559, 844
BccI CCATC 3 cut(s) 238, 317, 424
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 411
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 106
BfaI CTAG 4 cut(s) 78, 791, 909, 924
BglII AGATCT 1 cut(s) 442
BisI GCNGC 3 cut(s) 236, 573, 858
BlsI GCNGC 3 cut(s) 237, 574, 859
Bme1390I CCNGG 2 cut(s) 106, 411
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 4 cut(s) 9, 67, 93, 608
BmrFI CCNGG 2 cut(s) 106, 411
BmsI GCATC 7 cut(s) 113, 251, 338, 536, 584, 693, 736
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 106
BsaAI YACGTR 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 332, 410
BsaWI WCCGGW 2 cut(s) 112, 163
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 170, 223, 416, 616
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 269
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 332, 410
BseGI GGATG 6 cut(s) 309, 353, 527, 641, 684, 799
BseLI CCNNNNNNNGG 5 cut(s) 112, 170, 223, 416, 616
BseMI GCAATG 1 cut(s) 269
BseRI GAGGAG 3 cut(s) 263, 570, 599
BseXI GCAGC 2 cut(s) 559, 844
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 933
BshFI GGCC 1 cut(s) 373
BshNI GGYRCC 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 3 cut(s) 106, 113, 164
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 5 cut(s) 112, 170, 223, 416, 616
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 289
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 442, 590, 625, 961, 974
BspACI CCGC 4 cut(s) 69, 153, 235, 794
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 1 cut(s) 4
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 1 cut(s) 933
BspLI GGNNCC 4 cut(s) 9, 67, 93, 608
BspPI GGATC 1 cut(s) 956
BspT107I GGYRCC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 269
BssECI CCNNGG 2 cut(s) 332, 410
BssMI GATC 5 cut(s) 442, 590, 625, 961, 974
BssNAI GTATAC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 332
Bst1107I GTATAC 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 3 cut(s) 99, 340, 993
BstAPI GCANNNNNTGC 1 cut(s) 854
BstBAI YACGTR 1 cut(s) 869
BstDEI CTNAG 2 cut(s) 12, 449
BstEII GGTNACC 1 cut(s) 108
BstF5I GGATG 6 cut(s) 309, 353, 527, 641, 684, 799
BstFNI CGCG 1 cut(s) 933
BstKTI GATC 5 cut(s) 445, 593, 628, 964, 977
BstMBI GATC 5 cut(s) 442, 590, 625, 961, 974
BstMWI GCNNNNNNNGC 4 cut(s) 91, 671, 854, 899
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 659
BstPI GGTNACC 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 104, 409
BstSNI TACGTA 1 cut(s) 869
BstUI CGCG 1 cut(s) 933
BstV1I GCAGC 2 cut(s) 559, 844
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 368
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 1 cut(s) 373
BtgZI GCGATG 2 cut(s) 136, 366
BtsCI GGATG 6 cut(s) 309, 353, 527, 641, 684, 799
BtsIMutI CAGTG 1 cut(s) 95
CaiI CAGNNNCTG 2 cut(s) 410, 902
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 2 cut(s) 866, 984
CviAII CATG 2 cut(s) 569, 656
CviQI GTAC 2 cut(s) 866, 984
DdeI CTNAG 2 cut(s) 12, 449
DpnI GATC 5 cut(s) 444, 592, 627, 963, 976
DpnII GATC 5 cut(s) 442, 590, 625, 961, 974
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 287
Eco105I TACGTA 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 798
Eco81I CCTNAGG 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 332
FaeI CATG 2 cut(s) 572, 659
FaiI YATR 9 cut(s) 41, 47, 368, 534, 570, 657, 743, 763, 1009
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 568, 655
FauI CCCGC 2 cut(s) 160, 787
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 236, 573, 858
FokI GGATG 6 cut(s) 296, 360, 514, 628, 671, 786
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 3 cut(s) 236, 573, 858
FspBI CTAG 4 cut(s) 78, 791, 909, 924
GluI GCNGC 3 cut(s) 236, 573, 858
HaeIII GGCC 1 cut(s) 373
HapII CCGG 3 cut(s) 106, 113, 164
Hin1II CATG 2 cut(s) 572, 659
HincII GTYRAC 2 cut(s) 102, 343
HindII GTYRAC 2 cut(s) 102, 343
HinfI GANTC 1 cut(s) 770
HpaII CCGG 3 cut(s) 106, 113, 164
Hpy166II GTNNAC 5 cut(s) 102, 343, 368, 619, 866
Hpy188I TCNGA 5 cut(s) 278, 469, 500, 705, 778
Hpy188III TCNNGA 4 cut(s) 164, 319, 803, 972
Hpy8I GTNNAC 5 cut(s) 102, 343, 368, 619, 866
HpyAV CCTTC 3 cut(s) 157, 323, 597
HpyCH4III ACNGT 3 cut(s) 99, 340, 993
HpyCH4IV ACGT 1 cut(s) 868
HpyCH4V TGCA 6 cut(s) 404, 527, 575, 665, 749, 822
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 671, 854, 899
HpyF3I CTNAG 2 cut(s) 12, 449
HpySE526I ACGT 1 cut(s) 868
Hsp92II CATG 2 cut(s) 572, 659
Kpn2I TCCGGA 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 5 cut(s) 442, 590, 625, 961, 974
LmnI GCTCC 4 cut(s) 71, 284, 612, 899
Lsp1109I GCAGC 2 cut(s) 559, 844
LweI GCATC 7 cut(s) 113, 251, 338, 536, 584, 693, 736
MaeI CTAG 4 cut(s) 78, 791, 909, 924
MaeII ACGT 1 cut(s) 868
MaeIII GTNAC 2 cut(s) 108, 520
MalI GATC 5 cut(s) 444, 592, 627, 963, 976
MboI GATC 5 cut(s) 442, 590, 625, 961, 974
MboII GAAGA 5 cut(s) 253, 437, 718, 791, 794
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 3 cut(s) 394, 491, 543
MluI ACGCGT 1 cut(s) 931
MmeI TCCRAC 3 cut(s) 204, 541, 580
MroI TCCGGA 1 cut(s) 163
MseI TTAA 3 cut(s) 32, 944, 996
MslI CAYNNNNRTG 1 cut(s) 744
MspI CCGG 3 cut(s) 106, 113, 164
MspR9I CCNGG 2 cut(s) 106, 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 933
MwoI GCNNNNNNNGC 4 cut(s) 91, 671, 854, 899
NciI CCSGG 1 cut(s) 106
NdeII GATC 5 cut(s) 442, 590, 625, 961, 974
NlaIII CATG 2 cut(s) 572, 659
NlaIV GGNNCC 4 cut(s) 9, 67, 93, 608
NspI RCATGY 1 cut(s) 659
PfeI GAWTC 1 cut(s) 770
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 3 cut(s) 237, 574, 859
Ppu21I YACGTR 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 409
PspEI GGTNACC 1 cut(s) 108
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 4 cut(s) 9, 67, 93, 608
PspPI GGNCC 1 cut(s) 7
PstNI CAGNNNCTG 2 cut(s) 410, 902
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 2 cut(s) 867, 985
RsaNI GTAC 2 cut(s) 866, 984
RseI CAYNNNNRTG 1 cut(s) 744
SacI GAGCTC 1 cut(s) 289
SaqAI TTAA 3 cut(s) 32, 944, 996
SatI GCNGC 3 cut(s) 236, 573, 858
Sau3AI GATC 5 cut(s) 442, 590, 625, 961, 974
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 106, 411
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 7 cut(s) 113, 251, 338, 536, 584, 693, 736
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SnaBI TACGTA 1 cut(s) 869
Sse9I AATT 3 cut(s) 394, 491, 543
SsiI CCGC 4 cut(s) 69, 153, 235, 794
SspMI CTAG 4 cut(s) 78, 791, 909, 924
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 2 cut(s) 104, 409
StyI CCWWGG 1 cut(s) 332
TaaI ACNGT 3 cut(s) 99, 340, 993
TaiI ACGT 1 cut(s) 871
TaqI TCGA 2 cut(s) 328, 652
TasI AATT 3 cut(s) 394, 491, 543
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 3 cut(s) 32, 944, 996
Tru9I TTAA 3 cut(s) 32, 944, 996
TscAI CASTG 1 cut(s) 102
TseI GCWGC 2 cut(s) 572, 857
TspDTI ATGAA 4 cut(s) 369, 471, 630, 783
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 7
XceI RCATGY 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 367
XspI CTAG 4 cut(s) 78, 791, 909, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.