Rorug03G0253200

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005059
Physical Location & Seq
Forward (+)
24032490 .. 24041422
8933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0253200.1

Sequence Viewer

Length: 1881 bp
ATGGTCGACCAAAGGGAAGTTAGCCTTGAAGGTACACCAACATGGGCTGTATCAGTATTTGCTTTGTTTATCTTCTTGCTATCCTTTGCCATTGACGATTGTCTTCATCGTCTAACAAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTAAATAAGGCTTTGATGAAGTTCAGATCAGAAATGATGCAACTGGGTTTCGTATCTTTGCTTCTTACTCTGTCCGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAAGTTCCAGCTTCAAATACCAACTTCTCTGAAGTCACCACTCAAAATTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAACAGATGGAATCCAGCAGCTAAACATCTTTATCTCTGTGTTAGCAGTGTTTCATGTTCTCTACTGCATCTTGACTATGTTCCTTGGGATGGCCAAGATGAGGAAATGGAATGTATGGGAGGATGAGACTCGGACACTAAACTATCAGATTCTTAATGATCCAAGGAGGTTTCAACTCACTGATCAAACTCCTTTTGGGAAACGACATCTAAAGTTCTGGAGTAAACACCCTCTTCTTCTATGGCCTGCATGTTTCGCTCGGCAGTTCGGTGGCTCAATCTCAAAAGTCGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACTGCACAGAAGGTAGCAACTTCAACTTCCTGAACTTCCTTGTCAGAGCTTTCGACGACGATTTTGAGCAAGTGGTTGGAATCAAGCTTTGGATCTGGATCTCTTCAACTCTTTTCATGTTTTTCAATGCCCACGTGTTCTATAACTATTACTGGCTACCATTCATTCCCCTACTGATTGTTTTAGTAGTGGGAACAAAGCTGCAAGTCGTGATAACTAACATGTGCGTCGAGAGTTATGAGAAGAATCCTGTGATTCGAGGAAGCTTTGTTGTGAAGCTCAAAGATGGCTTATTTTGGTTCAGTCAACCCAATTGGCTTCTTCATCTTCTCCAATTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGACATGGTTTGAATTTGGCCAGAATTCATGCTTTAATCGAAGAACAAAAGATATCGTCATTAAGAGTAGTATGGGACTGGGTGTGCAGTTGATATGTGGTTATGTGACCCTCCCTCTTTACGCACTGGTTACCCAGATGGGGTCTGGCATGAGAAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTCAAAAACTGGCACAAGAATGCAAGACGCAGACTATCGAAGAATGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACATTTTTATTCAACCGATGACTCCTCATTTACAGACAATGAATGTAGCTACAAAACTCCTAATCCCACCACAGCGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCGCGTTCCAGCCGGTTTAAGTCCGAAGAATGGATCCACTACTTCAAGGAAATCCTCCGATTCTGCACATTTTTATTCAACTGATAACTCCTCATATCCAGACAATGAATGTAGCAACAAAACTCCTAATCCCACCATAGTGAGAAGCTCTTCTTTTACCTCCGAAATAATTGAAGAGCAAGTTCCACCCGGTTTAACTCCGAACCCGGATACTACAAGTTTACCAACTCCAGAAATCACCAGAGAGGAACAGAATCCCAACATCATCAACAAAATAATCTATGACGGTGAGATTTCTTTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATGAGCAAGAGAAGTGGGGAAATCACTTCAATAATTGAAGACGAACTTAACTTCGATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

626

Amino Acids

71.08

Weight (kDa)

8.46

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 461 8.4e-169 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AccII CGCG 1 cut(s) 1491
AciI CCGC 1 cut(s) 318
AclWI GGATC 7 cut(s) 557, 830, 836, 1338, 1351, 1515, 1528
AcoI YGGCCR 3 cut(s) 495, 726, 1126
AcsI RAATTY 3 cut(s) 1121, 1132, 1369
AcuI CTGAAG 1 cut(s) 372
AcvI CACGTG 1 cut(s) 865
AfaI GTAC 2 cut(s) 34, 228
AfiI CCNNNNNNNGG 6 cut(s) 154, 493, 504, 1113, 1248, 1517
AflIII ACRYGT 2 cut(s) 864, 951
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 1 cut(s) 1106
AjuI GAANNNNNNNTTGG 2 cut(s) 802, 834
AleI CACNNNNGTG 1 cut(s) 1625
Alw21I GWGCWC 1 cut(s) 1284
Alw26I GTCTC 3 cut(s) 253, 313, 524
AlwI GGATC 7 cut(s) 557, 830, 836, 1338, 1351, 1515, 1528
AoxI GGCC 5 cut(s) 495, 647, 726, 1126, 1294
ApeKI GCWGC 2 cut(s) 421, 931
ApoI RAATTY 3 cut(s) 1121, 1132, 1369
ArsI GACNNNNNNTTYG 2 cut(s) 776, 808
Asp700I GAANNNNTTC 2 cut(s) 1459, 1636
AspLEI GCGC 1 cut(s) 1491
AsuC2I CCSGG 2 cut(s) 1677, 1694
AsuHPI GGTGA 3 cut(s) 349, 1717, 1787
BalI TGGCCA 3 cut(s) 497, 728, 1128
BamHI GGATCC 2 cut(s) 1343, 1520
BanII GRGCYC 1 cut(s) 1284
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BbrPI CACGTG 1 cut(s) 865
BbsI GAAGAC 2 cut(s) 95, 1863
Bbv12I GWGCWC 1 cut(s) 1284
BbvI GCAGC 2 cut(s) 433, 918
BccI CCATC 5 cut(s) 404, 487, 1010, 1240, 1286
BciT130I CCWGG 1 cut(s) 1108
BciVI GTATCC 1 cut(s) 1690
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 2 cut(s) 1677, 1694
BcoDI GTCTC 3 cut(s) 253, 313, 524
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 2 cut(s) 303, 398
BfuI GTATCC 1 cut(s) 1690
BisI GCNGC 3 cut(s) 318, 422, 932
BlsI GCNGC 3 cut(s) 319, 423, 933
Bme1390I CCNGG 3 cut(s) 1108, 1677, 1694
BmiI GGNNCC 2 cut(s) 1345, 1522
BmrFI CCNGG 3 cut(s) 1108, 1677, 1694
BmrI ACTGGG 2 cut(s) 200, 1196
BmsI GCATC 2 cut(s) 174, 480
BmuI ACTGGG 2 cut(s) 200, 1196
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 95, 1863
BpmI CTGGAG 3 cut(s) 643, 1701, 1832
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 1677, 1694
BsaAI YACGTR 1 cut(s) 865
BsaBI GATNNNNATC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 291, 487, 566
Bsc4I CCNNNNNNNGG 6 cut(s) 154, 493, 504, 1113, 1248, 1517
Bse118I RCCGGY 1 cut(s) 1499
Bse1I ACTGG 5 cut(s) 195, 887, 1191, 1239, 1310
Bse8I GATNNNNATC 1 cut(s) 827
BseBI CCWGG 1 cut(s) 1108
BseDI CCNNGG 3 cut(s) 291, 487, 566
BseGI GGATG 2 cut(s) 498, 532
BseJI GATNNNNATC 1 cut(s) 827
BseLI CCNNNNNNNGG 6 cut(s) 154, 493, 504, 1113, 1248, 1517
BseMII CTCAG 1 cut(s) 1269
BseNI ACTGG 5 cut(s) 195, 887, 1191, 1239, 1310
BseRI GAGGAG 2 cut(s) 1390, 1567
BseXI GCAGC 2 cut(s) 433, 918
BsgI GTGCAG 4 cut(s) 718, 1214, 1359, 1536
Bsh1236I CGCG 1 cut(s) 1491
Bsh1285I CGRYCG 1 cut(s) 1876
BshFI GGCC 5 cut(s) 497, 649, 728, 1128, 1296
BsiEI CGRYCG 1 cut(s) 1876
BsiHKAI GWGCWC 1 cut(s) 1284
BsiSI CCGG 3 cut(s) 1500, 1677, 1694
BslFI GGGAC 1 cut(s) 1197
BslI CCNNNNNNNGG 6 cut(s) 154, 493, 504, 1113, 1248, 1517
BsmAI GTCTC 3 cut(s) 253, 313, 524
BsmFI GGGAC 1 cut(s) 1197
BsmI GAATGC 2 cut(s) 1097, 1321
BsnI GGCC 5 cut(s) 497, 649, 728, 1128, 1296
Bsp1286I GDGCHC 1 cut(s) 1284
Bsp143I GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
BspACI CCGC 1 cut(s) 318
BspANI GGCC 5 cut(s) 497, 649, 728, 1128, 1296
BspCNI CTCAG 1 cut(s) 1270
BspFNI CGCG 1 cut(s) 1491
BspLI GGNNCC 2 cut(s) 1345, 1522
BspPI GGATC 7 cut(s) 557, 830, 836, 1338, 1351, 1515, 1528
BspQI GCTCTTC 4 cut(s) 1465, 1479, 1642, 1656
BsrFI RCCGGY 1 cut(s) 1499
BsrI ACTGG 5 cut(s) 195, 887, 1191, 1239, 1310
BssAI RCCGGY 1 cut(s) 1499
BssECI CCNNGG 3 cut(s) 291, 487, 566
BssMI GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
BssT1I CCWWGG 2 cut(s) 487, 566
Bst2UI CCWGG 1 cut(s) 1108
Bst4CI ACNGT 1 cut(s) 1775
Bst6I CTCTTC 6 cut(s) 642, 838, 1465, 1479, 1642, 1656
BstBAI YACGTR 1 cut(s) 865
BstC8I GCNNGC 2 cut(s) 651, 1095
BstDEI CTNAG 1 cut(s) 1278
BstEII GGTNACC 1 cut(s) 1237
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 498, 532
BstFNI CGCG 1 cut(s) 1491
BstHHI GCGC 1 cut(s) 1491
BstKTI GATC 8 cut(s) 176, 565, 589, 825, 831, 1346, 1523, 1876
BstMAI GTCTC 3 cut(s) 253, 313, 524
BstMBI GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
BstMCI CGRYCG 1 cut(s) 1876
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 1 cut(s) 1108
BstNSI RCATGY 2 cut(s) 657, 955
BstPAI GACNNNNGTC 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 1237
BstSCI CCNGG 3 cut(s) 1106, 1675, 1692
BstUI CGCG 1 cut(s) 1491
BstV1I GCAGC 2 cut(s) 433, 918
BstV2I GAAGAC 2 cut(s) 95, 1863
BstX2I RGATCY 4 cut(s) 822, 828, 1343, 1520
BstYI RGATCY 4 cut(s) 822, 828, 1343, 1520
BsuI GTATCC 1 cut(s) 1690
BsuRI GGCC 5 cut(s) 497, 649, 728, 1128, 1296
BtsCI GGATG 2 cut(s) 498, 532
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 3 cut(s) 456, 582, 1232
Cac8I GCNNGC 2 cut(s) 651, 1095
CfoI GCGC 1 cut(s) 1491
Cfr10I RCCGGY 1 cut(s) 1499
CseI GACGC 2 cut(s) 946, 1332
Csp6I GTAC 2 cut(s) 33, 227
CspCI CAANNNNNGTGG 2 cut(s) 1813, 1848
CviQI GTAC 2 cut(s) 33, 227
DdeI CTNAG 1 cut(s) 1278
DpnI GATC 8 cut(s) 175, 564, 588, 824, 830, 1345, 1522, 1875
DpnII GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
EaeI YGGCCR 3 cut(s) 495, 726, 1126
Eam1104I CTCTTC 6 cut(s) 642, 838, 1465, 1479, 1642, 1656
EarI CTCTTC 6 cut(s) 642, 838, 1465, 1479, 1642, 1656
Ecl136II GAGCTC 1 cut(s) 1282
Eco130I CCWWGG 2 cut(s) 487, 566
Eco24I GRGCYC 1 cut(s) 1284
Eco32I GATATC 1 cut(s) 1162
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 1 cut(s) 372
Eco72I CACGTG 1 cut(s) 865
Eco91I GGTNACC 1 cut(s) 1237
EcoICRI GAGCTC 1 cut(s) 1282
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 1237
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 1 cut(s) 1106
EcoRV GATATC 1 cut(s) 1162
EcoT14I CCWWGG 2 cut(s) 487, 566
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 2 cut(s) 487, 566
FalI AAGNNNNNCTT 8 cut(s) 9, 41, 1447, 1479, 1624, 1656, 1848, 1880
FaqI GGGAC 1 cut(s) 1197
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 318, 422, 932
FokI GGATG 2 cut(s) 505, 539
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 3 cut(s) 318, 422, 932
FspBI CTAG 2 cut(s) 303, 398
GlaI GCGC 1 cut(s) 1490
GluI GCNGC 3 cut(s) 318, 422, 932
GsuI CTGGAG 3 cut(s) 643, 1701, 1832
HaeIII GGCC 5 cut(s) 497, 649, 728, 1128, 1296
HapII CCGG 3 cut(s) 1500, 1677, 1694
HgaI GACGC 2 cut(s) 946, 1332
HhaI GCGC 1 cut(s) 1491
Hin6I GCGC 1 cut(s) 1489
HinP1I GCGC 1 cut(s) 1489
HincII GTYRAC 3 cut(s) 7, 405, 1037
HindII GTYRAC 3 cut(s) 7, 405, 1037
HindIII AAGCTT 2 cut(s) 815, 994
HpaII CCGG 3 cut(s) 1500, 1677, 1694
HphI GGTGA 3 cut(s) 349, 1717, 1787
Hpy166II GTNNAC 6 cut(s) 7, 35, 405, 629, 1037, 1709
Hpy8I GTNNAC 6 cut(s) 7, 35, 405, 629, 1037, 1709
Hpy99I CGWCG 3 cut(s) 788, 791, 962
HpyAV CCTTC 3 cut(s) 23, 126, 734
HpyCH4III ACNGT 1 cut(s) 1775
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 9 cut(s) 187, 471, 653, 735, 934, 1195, 1319, 1376, 1553
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 1 cut(s) 1278
HpySE526I ACGT 1 cut(s) 864
HspAI GCGC 1 cut(s) 1489
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
LguI GCTCTTC 4 cut(s) 1465, 1479, 1642, 1656
LmnI GCTCC 1 cut(s) 1791
Lsp1109I GCAGC 2 cut(s) 433, 918
LweI GCATC 2 cut(s) 174, 480
MaeI CTAG 2 cut(s) 303, 398
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 355, 1213, 1237
MalI GATC 8 cut(s) 175, 564, 588, 824, 830, 1345, 1522, 1875
MboI GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
MfeI CAATTG 1 cut(s) 1042
MflI RGATCY 4 cut(s) 822, 828, 1343, 1520
MhlI GDGCHC 1 cut(s) 1284
MlsI TGGCCA 3 cut(s) 497, 728, 1128
MluNI TGGCCA 3 cut(s) 497, 728, 1128
MlyI GAGTC 4 cut(s) 290, 526, 697, 1391
MmeI TCCRAC 2 cut(s) 787, 1778
Mox20I TGGCCA 3 cut(s) 497, 728, 1128
MroXI GAANNNNTTC 2 cut(s) 1459, 1636
MscI TGGCCA 3 cut(s) 497, 728, 1128
MseI TTAA 8 cut(s) 126, 558, 1143, 1170, 1505, 1682, 1805, 1866
MslI CAYNNNNRTG 3 cut(s) 40, 1314, 1625
Msp20I TGGCCA 3 cut(s) 497, 728, 1128
MspA1I CMGCKG 1 cut(s) 1093
MspI CCGG 3 cut(s) 1500, 1677, 1694
MspR9I CCNGG 3 cut(s) 1108, 1677, 1694
MunI CAATTG 1 cut(s) 1042
Mva1269I GAATGC 2 cut(s) 1097, 1321
MvaI CCWGG 1 cut(s) 1108
MvnI CGCG 1 cut(s) 1491
MwoI GCNNNNNNNGC 1 cut(s) 659
NciI CCSGG 2 cut(s) 1677, 1694
NdeII GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
NlaIV GGNNCC 2 cut(s) 1345, 1522
NmeAIII GCCGAG 1 cut(s) 643
NmuCI GTSAC 2 cut(s) 355, 1213
NspI RCATGY 2 cut(s) 657, 955
OliI CACNNNNGTG 1 cut(s) 1625
PciI ACATGT 1 cut(s) 951
PciSI GCTCTTC 4 cut(s) 1465, 1479, 1642, 1656
PctI GAATGC 2 cut(s) 1097, 1321
PdmI GAANNNNTTC 2 cut(s) 1459, 1636
PfeI GAWTC 7 cut(s) 414, 553, 810, 976, 985, 1547, 1741
PkrI GCNGC 3 cut(s) 319, 423, 933
Ple19I CGATCG 1 cut(s) 1876
PleI GAGTC 4 cut(s) 290, 526, 697, 1391
PmaCI CACGTG 1 cut(s) 865
PmlI CACGTG 1 cut(s) 865
PpsI GAGTC 4 cut(s) 290, 526, 697, 1391
Ppu21I YACGTR 1 cut(s) 865
PscI ACATGT 1 cut(s) 951
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 1284
Psp6I CCWGG 1 cut(s) 1106
PspCI CACGTG 1 cut(s) 865
PspEI GGTNACC 1 cut(s) 1237
PspGI CCWGG 1 cut(s) 1106
PspN4I GGNNCC 2 cut(s) 1345, 1522
PsuI RGATCY 4 cut(s) 822, 828, 1343, 1520
PvuI CGATCG 1 cut(s) 1876
PvuII CAGCTG 1 cut(s) 1093
RsaI GTAC 2 cut(s) 34, 228
RsaNI GTAC 2 cut(s) 33, 227
RseI CAYNNNNRTG 3 cut(s) 40, 1314, 1625
SacI GAGCTC 1 cut(s) 1284
SalI GTCGAC 1 cut(s) 5
SapI GCTCTTC 4 cut(s) 1465, 1479, 1642, 1656
SaqAI TTAA 8 cut(s) 126, 558, 1143, 1170, 1505, 1682, 1805, 1866
SatI GCNGC 3 cut(s) 318, 422, 932
Sau3AI GATC 8 cut(s) 173, 562, 586, 822, 828, 1343, 1520, 1873
SchI GAGTC 4 cut(s) 290, 526, 697, 1391
ScrFI CCNGG 3 cut(s) 1108, 1677, 1694
SduI GDGCHC 1 cut(s) 1284
SfaNI GCATC 2 cut(s) 174, 480
SmiMI CAYNNNNRTG 3 cut(s) 40, 1314, 1625
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
SpeI ACTAGT 1 cut(s) 302
SsiI CCGC 1 cut(s) 318
SspMI CTAG 2 cut(s) 303, 398
SstI GAGCTC 1 cut(s) 1284
StyD4I CCNGG 3 cut(s) 1106, 1675, 1692
StyI CCWWGG 2 cut(s) 487, 566
TaaI ACNGT 1 cut(s) 1775
TaiI ACGT 1 cut(s) 867
TaqI TCGA 9 cut(s) 6, 236, 693, 783, 960, 988, 1147, 1334, 1872
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 7 cut(s) 414, 553, 810, 976, 985, 1547, 1741
Tru1I TTAA 8 cut(s) 126, 558, 1143, 1170, 1505, 1682, 1805, 1866
Tru9I TTAA 8 cut(s) 126, 558, 1143, 1170, 1505, 1682, 1805, 1866
TscAI CASTG 3 cut(s) 456, 589, 1239
TseFI GTSAC 2 cut(s) 355, 1213
TseI GCWGC 2 cut(s) 421, 931
Tsp45I GTSAC 2 cut(s) 355, 1213
TspRI CASTG 3 cut(s) 456, 589, 1239
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 3 cut(s) 1121, 1132, 1369
XceI RCATGY 2 cut(s) 657, 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 2 cut(s) 1459, 1636
XspI CTAG 2 cut(s) 303, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.