Rroxscaffold_6G00392430

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005059
Physical Location & Seq
Forward (+)
11988073 .. 12001770
13698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392430.1

Sequence Viewer

Length: 1380 bp
ATGGAGGGGAGAGCAAGATGCTTCACATTTCTCGGCGTCGGAGCTCTGCTGGGCTCCGTCTCCACCTTTCTTCTGCTCAAGCTTCTACCAAGGCCAGCACTCCATATGAATGATATTAATGGTAAAGGGTTGGCATCTGAGACACCTACAAATTGCAGTGCAGTTACTGGGAGCGGAAATAGCAAGTTAGCTGGTGTAGACCTTCTAAATGATGAGATAGTCTGTGAACAACTAACAAGAAACATTCAATTCTTTGGACTTGAACCTCAACAAAAAGTGACTGCTTCCTATGTTGTGGTCATTGGTCTTGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGGTCGGGGGTTGGCAGGCTTCTCCTTGTGGACTTTGATCAGGTATCAGTTTCATCACTTAATCGACATGCTGTTGCAACAAGAGCTGACGTTGGTATCCCAAAAGCCCAGTGTCTAAAAGATCATTTCTTGTCTATCTTCCCAGAGTGTCACATAGAAGCAAAGGTCCTGTTATACAATGCATCATCTGAGGAAGAGATTCTTTCGGGTCATCCTGATTTTGTTTTGGACTGCATTGACAACATTGATACAAAGGTGGCACTTCTTGCGGCATGTGTACGTAGGGGTTTAAAGGTCCTATCAGCCACAGGAGCTGGTGCTAGAGCTGACCCGACAAGAATACGCATTGCTGATTTAAGACAATCGACAAATGACCCATTATCTCGAGCTGTGAGACACCGTTTGAAGAGAGAGTATGGCATTGAAGGTGGCATTCCTGTTGTATTTTCATTGGAGAAACCTAAGGCAAAGCTTCTTCCATTTAAAGGACAAAGTGGAGAAGAAGAAAATCCTTCAGATTACCAGATAGTACCAGGGTTTAGGGTTCGCATAATACCTGTCCTTGGTACCATTCCTGCAATATTCGGCCAAGTGATGGCCTCCTATGTGTTGACACAACTTGCAGAATTGCAAGTTCAATTGGAACCTGTGATAAACTTGGACATTGACCATTACCGGATGCTTCACCAACGTCTTATTGAGCATGAGGAGTCGTTGTATGGCACTGCCTTGCATGTCCAGGTAGATGTTGAAGAAGTGATGTATGTTGCAAAAGAGTTGTGGCATGGAAGAAGTGCTAGAGAGCCATTTGCTAAAGATGTAGGACGTAGCATGTGGCGATCGGTGAATGAATTAATGCTTTTGAGGTGGGACCGGGAAAAGCCGGCATCTGTGTCCAACTTAATTCTCTTGAAATTTAAAGAGGCAGATGAACATGAGTCCAGGACTCTTGAAGATATAAAAGAAGAGGAACCAGAATTTTTTGTTCGAGTTACATCTGTTCTGAAGCGAGCCGAACTAGAATTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

459

Amino Acids

50.82

Weight (kDa)

6.05

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 79 - 334 2.4e-44 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 940
AccBSI CCGCTC 1 cut(s) 174
AccI GTMKAC 1 cut(s) 198
AciI CCGC 3 cut(s) 174, 346, 614
AcoI YGGCCR 1 cut(s) 931
AcsI RAATTY 3 cut(s) 1259, 1322, 1367
AcuI CTGAAG 2 cut(s) 843, 1370
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 624, 876, 913
AfiI CCNNNNNNNGG 3 cut(s) 830, 908, 940
AgsI TTSAA 8 cut(s) 248, 263, 751, 770, 983, 1097, 1258, 1298
AjnI CCWGG 3 cut(s) 877, 1083, 1286
AjuI GAANNNNNNNTTGG 1 cut(s) 1353
AluBI AGCT 8 cut(s) 44, 82, 191, 431, 659, 671, 734, 817
AluI AGCT 8 cut(s) 44, 82, 191, 431, 659, 671, 734, 817
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 3 cut(s) 64, 134, 733
AlwNI CAGNNNCTG 2 cut(s) 167, 659
Ama87I CYCGRG 1 cut(s) 729
AoxI GGCC 3 cut(s) 92, 931, 942
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 3 cut(s) 1259, 1322, 1367
AseI ATTAAT 2 cut(s) 117, 1199
Asp700I GAANNNNTTC 1 cut(s) 543
Asp718I GGTACC 1 cut(s) 911
AspS9I GGNCC 3 cut(s) 511, 640, 1216
AsuC2I CCSGG 1 cut(s) 1220
AsuHPI GGTGA 2 cut(s) 1022, 1201
AvaI CYCGRG 1 cut(s) 729
AvaII GGWCC 3 cut(s) 511, 640, 1216
AxyI CCTNAGG 1 cut(s) 807
BaeI ACNNNNGTAYC 4 cut(s) 424, 457, 585, 618
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 2 cut(s) 46, 56
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 316
BccI CCATC 1 cut(s) 934
BciT130I CCWGG 3 cut(s) 879, 1085, 1288
BciVI GTATCC 1 cut(s) 452
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 1220
BcoDI GTCTC 3 cut(s) 64, 134, 733
BfaI CTAG 3 cut(s) 666, 1143, 1364
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 2 cut(s) 330, 615
BlsI GCNGC 2 cut(s) 331, 616
Bme1390I CCNGG 4 cut(s) 879, 1085, 1220, 1288
Bme18I GGWCC 3 cut(s) 511, 640, 1216
BmeT110I CYCGRG 1 cut(s) 729
BmgT120I GGNCC 3 cut(s) 511, 640, 1216
BmiI GGNNCC 5 cut(s) 55, 913, 990, 1217, 1317
BmrFI CCNGG 4 cut(s) 879, 1085, 1220, 1288
BmrI ACTGGG 2 cut(s) 177, 448
BmsI GCATC 6 cut(s) 8, 143, 341, 536, 1014, 1241
BmuI ACTGGG 2 cut(s) 177, 448
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 1220
BsaAI YACGTR 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 89, 878, 907
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 3 cut(s) 830, 908, 940
Bse118I RCCGGY 1 cut(s) 1228
Bse1I ACTGG 2 cut(s) 172, 454
Bse21I CCTNAGG 1 cut(s) 807
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 3 cut(s) 879, 1085, 1288
BseDI CCNNGG 3 cut(s) 89, 878, 907
BseGI GGATG 2 cut(s) 556, 1029
BseLI CCNNNNNNNGG 3 cut(s) 830, 908, 940
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 2 cut(s) 129, 525
BseNI ACTGG 2 cut(s) 172, 454
BseRI GAGGAG 2 cut(s) 327, 1067
BseXI GCAGC 1 cut(s) 316
BseYI CCCAGC 1 cut(s) 49
BsgI GTGCAG 1 cut(s) 180
Bsh1285I CGRYCG 2 cut(s) 350, 1187
BshFI GGCC 3 cut(s) 94, 933, 944
BshNI GGYRCC 1 cut(s) 911
BsiEI CGRYCG 2 cut(s) 350, 1187
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 729
BsiSI CCGG 3 cut(s) 1021, 1219, 1229
BslFI GGGAC 1 cut(s) 1229
BslI CCNNNNNNNGG 3 cut(s) 830, 908, 940
BsmAI GTCTC 3 cut(s) 64, 134, 733
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 1229
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 94, 933, 944
BsoBI CYCGRG 1 cut(s) 729
Bsp1286I GDGCHC 2 cut(s) 46, 56
Bsp143I GATC 3 cut(s) 382, 466, 1184
BspACI CCGC 3 cut(s) 174, 346, 614
BspANI GGCC 3 cut(s) 94, 933, 944
BspCNI CTCAG 2 cut(s) 130, 526
BspLI GGNNCC 5 cut(s) 55, 913, 990, 1217, 1317
BspT107I GGYRCC 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 690
BsrFI RCCGGY 1 cut(s) 1228
BsrI ACTGG 2 cut(s) 172, 454
BssAI RCCGGY 1 cut(s) 1228
BssECI CCNNGG 3 cut(s) 89, 878, 907
BssMI GATC 3 cut(s) 382, 466, 1184
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 89, 907
Bst2UI CCWGG 3 cut(s) 879, 1085, 1288
Bst4CI ACNGT 1 cut(s) 746
Bst6I CTCTTC 3 cut(s) 534, 746, 1305
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 611
BstBAI YACGTR 1 cut(s) 626
BstC8I GCNNGC 4 cut(s) 96, 362, 1230, 1356
BstDEI CTNAG 3 cut(s) 138, 534, 807
BstF5I GGATG 2 cut(s) 556, 1029
BstKTI GATC 3 cut(s) 385, 469, 1187
BstMAI GTCTC 3 cut(s) 64, 134, 733
BstMBI GATC 3 cut(s) 382, 466, 1184
BstMCI CGRYCG 2 cut(s) 350, 1187
BstMWI GCNNNNNNNGC 4 cut(s) 180, 428, 611, 656
BstNI CCWGG 3 cut(s) 879, 1085, 1288
BstNSI RCATGY 4 cut(s) 416, 621, 1082, 1180
BstSCI CCNGG 4 cut(s) 877, 1083, 1218, 1286
BstSNI TACGTA 1 cut(s) 626
BstV1I GCAGC 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 807
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 3 cut(s) 94, 933, 944
BtsCI GGATG 2 cut(s) 556, 1029
BtsI GCAGTG 2 cut(s) 163, 1068
BtsIMutI CAGTG 3 cut(s) 163, 461, 1068
Cac8I GCNNGC 4 cut(s) 96, 362, 1230, 1356
CaiI CAGNNNCTG 2 cut(s) 167, 659
Cfr10I RCCGGY 1 cut(s) 1228
Cfr13I GGNCC 3 cut(s) 511, 640, 1216
CseI GACGC 1 cut(s) 25
Csp6I GTAC 3 cut(s) 623, 875, 912
CviAII CATG 8 cut(s) 326, 413, 618, 1049, 1079, 1130, 1177, 1280
CviQI GTAC 3 cut(s) 623, 875, 912
DdeI CTNAG 3 cut(s) 138, 534, 807
DpnI GATC 3 cut(s) 384, 468, 1186
DpnII GATC 3 cut(s) 382, 466, 1184
DraI TTTAAA 3 cut(s) 636, 829, 1264
EaeI YGGCCR 1 cut(s) 931
Eam1104I CTCTTC 3 cut(s) 534, 746, 1305
EarI CTCTTC 3 cut(s) 534, 746, 1305
Ecl136II GAGCTC 1 cut(s) 44
Eco105I TACGTA 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 89, 907
Eco24I GRGCYC 2 cut(s) 46, 56
Eco47I GGWCC 3 cut(s) 511, 640, 1216
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 843, 1370
Eco81I CCTNAGG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 44
EcoO109I RGGNCCY 2 cut(s) 511, 640
EcoRII CCWGG 3 cut(s) 877, 1083, 1286
EcoT14I CCWWGG 2 cut(s) 89, 907
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 2 cut(s) 46, 56
ErhI CCWWGG 2 cut(s) 89, 907
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 8 cut(s) 329, 416, 621, 1052, 1082, 1133, 1180, 1283
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 1 cut(s) 1229
FatI CATG 8 cut(s) 325, 412, 617, 1048, 1078, 1129, 1176, 1279
FauNDI CATATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 330, 615
FokI GGATG 2 cut(s) 543, 1036
FriOI GRGCYC 2 cut(s) 46, 56
Fsp4HI GCNGC 2 cut(s) 330, 615
FspBI CTAG 3 cut(s) 666, 1143, 1364
GluI GCNGC 2 cut(s) 330, 615
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 3 cut(s) 94, 933, 944
HapII CCGG 3 cut(s) 1021, 1219, 1229
HgaI GACGC 1 cut(s) 25
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 8 cut(s) 329, 416, 621, 1052, 1082, 1133, 1180, 1283
HincII GTYRAC 1 cut(s) 957
HindII GTYRAC 1 cut(s) 957
HindIII AAGCTT 2 cut(s) 80, 815
HinfI GANTC 4 cut(s) 544, 1055, 1283, 1291
HpaII CCGG 3 cut(s) 1021, 1219, 1229
HphI GGTGA 2 cut(s) 1022, 1201
Hpy166II GTNNAC 5 cut(s) 199, 227, 376, 623, 957
Hpy188I TCNGA 5 cut(s) 41, 139, 535, 862, 1350
Hpy188III TCNNGA 4 cut(s) 560, 729, 1255, 1295
Hpy8I GTNNAC 5 cut(s) 199, 227, 376, 623, 957
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 212, 764, 867
HpyCH4III ACNGT 1 cut(s) 746
HpyCH4IV ACGT 4 cut(s) 435, 625, 1036, 1171
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 428, 611, 656
HpyF3I CTNAG 3 cut(s) 138, 534, 807
HpySE526I ACGT 4 cut(s) 435, 625, 1036, 1171
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 8 cut(s) 329, 416, 621, 1052, 1082, 1133, 1180, 1283
KpnI GGTACC 1 cut(s) 915
KroI GCCGGC 1 cut(s) 1228
KroNI GCCGGC 1 cut(s) 1230
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 382, 466, 1184
LmnI GCTCC 4 cut(s) 41, 59, 171, 656
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 6 cut(s) 8, 143, 341, 536, 1014, 1241
MaeI CTAG 3 cut(s) 666, 1143, 1364
MaeII ACGT 4 cut(s) 435, 625, 1036, 1171
MaeIII GTNAC 4 cut(s) 163, 277, 494, 1336
MalI GATC 3 cut(s) 384, 468, 1186
MbiI CCGCTC 1 cut(s) 174
MboI GATC 3 cut(s) 382, 466, 1184
MfeI CAATTG 1 cut(s) 983
MhlI GDGCHC 2 cut(s) 46, 56
MluCI AATT 9 cut(s) 151, 248, 971, 983, 1196, 1248, 1259, 1322, 1367
MlyI GAGTC 3 cut(s) 1064, 1285, 1292
MmeI TCCRAC 3 cut(s) 19, 298, 1266
MnlI CCTC 8 cut(s) 276, 305, 529, 955, 1045, 1203, 1261, 1306
Mph1103I ATGCAT 1 cut(s) 529
MroNI GCCGGC 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 8 cut(s) 117, 405, 635, 701, 828, 1199, 1247, 1263
MslI CAYNNNNRTG 1 cut(s) 108
MspI CCGG 3 cut(s) 1021, 1219, 1229
MspR9I CCNGG 4 cut(s) 879, 1085, 1220, 1288
MunI CAATTG 1 cut(s) 983
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 3 cut(s) 879, 1085, 1288
MwoI GCNNNNNNNGC 4 cut(s) 180, 428, 611, 656
NaeI GCCGGC 1 cut(s) 1230
NciI CCSGG 1 cut(s) 1220
NdeI CATATG 1 cut(s) 105
NdeII GATC 3 cut(s) 382, 466, 1184
NgoMIV GCCGGC 1 cut(s) 1228
NlaIII CATG 8 cut(s) 329, 416, 621, 1052, 1082, 1133, 1180, 1283
NlaIV GGNNCC 5 cut(s) 55, 913, 990, 1217, 1317
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 2 cut(s) 277, 494
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 4 cut(s) 416, 621, 1082, 1180
PaeR7I CTCGAG 1 cut(s) 729
PctI GAATGC 1 cut(s) 777
PdiI GCCGGC 1 cut(s) 1230
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 940
PfoI TCCNGGA 1 cut(s) 1286
PkrI GCNGC 2 cut(s) 331, 616
Ple19I CGATCG 1 cut(s) 1187
PleI GAGTC 3 cut(s) 1063, 1285, 1291
PpsI GAGTC 3 cut(s) 1063, 1285, 1291
Ppu21I YACGTR 1 cut(s) 626
PpuMI RGGWCCY 2 cut(s) 511, 640
PshBI ATTAAT 2 cut(s) 117, 1199
Psp124BI GAGCTC 1 cut(s) 46
Psp5II RGGWCCY 2 cut(s) 511, 640
Psp6I CCWGG 3 cut(s) 877, 1083, 1286
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 3 cut(s) 877, 1083, 1286
PspN4I GGNNCC 5 cut(s) 55, 913, 990, 1217, 1317
PspPI GGNCC 3 cut(s) 511, 640, 1216
PspPPI RGGWCCY 2 cut(s) 511, 640
PstNI CAGNNNCTG 2 cut(s) 167, 659
PvuI CGATCG 1 cut(s) 1187
RsaI GTAC 3 cut(s) 624, 876, 913
RsaNI GTAC 3 cut(s) 623, 875, 912
RseI CAYNNNNRTG 1 cut(s) 108
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 8 cut(s) 117, 405, 635, 701, 828, 1199, 1247, 1263
SatI GCNGC 2 cut(s) 330, 615
Sau3AI GATC 3 cut(s) 382, 466, 1184
Sau96I GGNCC 3 cut(s) 511, 640, 1216
SchI GAGTC 3 cut(s) 1064, 1285, 1292
ScrFI CCNGG 4 cut(s) 879, 1085, 1220, 1288
SduI GDGCHC 2 cut(s) 46, 56
SfaNI GCATC 6 cut(s) 8, 143, 341, 536, 1014, 1241
Sfr274I CTCGAG 1 cut(s) 729
SinI GGWCC 3 cut(s) 511, 640, 1216
SlaI CTCGAG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 108
SmlI CTYRAG 2 cut(s) 77, 729
SmoI CTYRAG 2 cut(s) 77, 729
SnaBI TACGTA 1 cut(s) 626
Sse9I AATT 9 cut(s) 151, 248, 971, 983, 1196, 1248, 1259, 1322, 1367
SsiI CCGC 3 cut(s) 174, 346, 614
SspI AATATT 1 cut(s) 927
SspMI CTAG 3 cut(s) 666, 1143, 1364
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 4 cut(s) 877, 1083, 1218, 1286
StyI CCWWGG 2 cut(s) 89, 907
TaaI ACNGT 1 cut(s) 746
TaiI ACGT 4 cut(s) 438, 628, 1039, 1174
TaqI TCGA 4 cut(s) 409, 710, 730, 1333
TasI AATT 9 cut(s) 151, 248, 971, 983, 1196, 1248, 1259, 1322, 1367
TauI GCSGC 1 cut(s) 617
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 8 cut(s) 117, 405, 635, 701, 828, 1199, 1247, 1263
Tru9I TTAA 8 cut(s) 117, 405, 635, 701, 828, 1199, 1247, 1263
TscAI CASTG 3 cut(s) 163, 461, 1075
TseFI GTSAC 2 cut(s) 277, 494
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 2 cut(s) 277, 494
TspDTI ATGAA 5 cut(s) 122, 387, 783, 1209, 1290
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 3 cut(s) 163, 461, 1075
Van91I CCANNNNNTGG 1 cut(s) 940
VpaK11BI GGWCC 3 cut(s) 511, 640, 1216
VspI ATTAAT 2 cut(s) 117, 1199
XapI RAATTY 3 cut(s) 1259, 1322, 1367
XceI RCATGY 4 cut(s) 416, 621, 1082, 1180
XhoI CTCGAG 1 cut(s) 729
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 3 cut(s) 666, 1143, 1364
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.