Rh3CG336300

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005059
Physical Location & Seq
Reverse (-)
37536276 .. 37550943
14668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG336300.1

Sequence Viewer

Length: 1167 bp
ATGTTTTATCTTTCTGGTTTCTTTAGAAACATTCAATTCTTTGGACTTGAACCTCAACAAAAAGTGACTGCTTCCTATGTTGTGGTCATTGGTCTTGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGGTCGGGGGTTGGCAGGCTTCTCCTTGTGGACTTTGATCAGGTATCAGTTTCATCACTTAATCGACATGCTGTTGCAACAAGAGCTGACGTTGGTATCCCAAAAGCCCAGTGTCTAAAAGATCATTTCTTGTCTATCTTCCCAGAGTGTCACATAGAAGCAAAGGTCCTGTTATACAATGCATCGTCTGAGGAAGAGATTCTTTCGGGTCATCCTGATTTTGTTTTGGACTGCATTGACAACATTGATACAAAGGTGGCACTTCTTGCGGCATGTGTACGTAGGGGTTTAAAGGTCCTATCAGCCACAGGAGCTGGTGCTAGAGCTGACCCAACAAGAATACGCATTGCTGATTTAAGACAATCGACAAATGACCCATTATCTCGAGCTGTGAGACACCGTTTGAAGAGAGAGTATGGCATTGATGGTGGCATTCCTGTTGTATTTTCATTGGAGAAACCTAAGGCAAAGCTTCTTCCATTTAAAGGACAAAGTGGAGAAGAAGAAAATCCTTCAGATTACCAGATAGTACCAGGGTTTAGGGTTCGCATAATACCTGTCCTTGGTACCATTCCTGCAATATTTGGCCAAGTGATGGCCTCCTATGTGTTGACACAACTTGCAGAATTGCAAGTTCAATTGGAACCTGTGATAAACTTGGACATTGACCATTACCGGATGCTTCACCAACGTCTTATTGAGCATGAGGAGTCATTGTATGGCACTGCCTTGCATGTCCTGGTAGATGTTGAAGAAGTGATGTATGTTGCAAAAGAGTTGTGGCATGGAAGAAGTGCTAGAGAGCCATTTGCTAAAGATGTAGGACGTAGCATGTGGCGATCGGTGAATGAATTAATGCTTTTGAGGTGGGACCGGGAAAAGCCGGCATCTGTGTCCAACTTAATTCTATTGAAATTTAAAGAGGCAGATGAACATGAATCCAGGACTCTTGAAGATATAAAAGAGGAGGAACCAGAATTTTTTGTTCGAGTTACATCTGTTCTGAAGCGAGCCGAAGTAGAATTTGGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

388

Amino Acids

43.54

Weight (kDa)

6.09

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 9 - 263 4.4e-44 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 698
AccB7I CCANNNNNTGG 1 cut(s) 727
AciI CCGC 2 cut(s) 133, 401
AcoI YGGCCR 1 cut(s) 718
AcsI RAATTY 3 cut(s) 1046, 1109, 1154
AcuI CTGAAG 2 cut(s) 630, 1157
AfaI GTAC 3 cut(s) 411, 663, 700
AfiI CCNNNNNNNGG 3 cut(s) 617, 695, 727
AgsI TTSAA 7 cut(s) 35, 50, 538, 770, 884, 1045, 1085
AjnI CCWGG 3 cut(s) 664, 870, 1073
AjuI GAANNNNNNNTTGG 1 cut(s) 1140
AluBI AGCT 5 cut(s) 218, 446, 458, 521, 604
AluI AGCT 5 cut(s) 218, 446, 458, 521, 604
Alw26I GTCTC 1 cut(s) 520
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 516
AoxI GGCC 2 cut(s) 718, 729
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 3 cut(s) 1046, 1109, 1154
AseI ATTAAT 1 cut(s) 986
Asp700I GAANNNNTTC 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 698
AspS9I GGNCC 3 cut(s) 298, 427, 1003
AsuC2I CCSGG 1 cut(s) 1007
AsuHPI GGTGA 2 cut(s) 809, 988
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 3 cut(s) 298, 427, 1003
AxyI CCTNAGG 1 cut(s) 594
BaeI ACNNNNGTAYC 4 cut(s) 211, 244, 372, 405
BalI TGGCCA 1 cut(s) 720
BanI GGYRCC 1 cut(s) 698
BbvI GCAGC 1 cut(s) 103
BccI CCATC 2 cut(s) 551, 721
BciT130I CCWGG 3 cut(s) 666, 872, 1075
BciVI GTATCC 1 cut(s) 239
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 1007
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 2 cut(s) 453, 930
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 2 cut(s) 117, 402
BlsI GCNGC 2 cut(s) 118, 403
Bme1390I CCNGG 4 cut(s) 666, 872, 1007, 1075
Bme18I GGWCC 3 cut(s) 298, 427, 1003
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 3 cut(s) 298, 427, 1003
BmiI GGNNCC 4 cut(s) 700, 777, 1004, 1104
BmrFI CCNGG 4 cut(s) 666, 872, 1007, 1075
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 4 cut(s) 128, 323, 801, 1028
BmuI ACTGGG 1 cut(s) 235
BpuMI CCSGG 1 cut(s) 1007
BsaAI YACGTR 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 665, 694
BsaWI WCCGGW 1 cut(s) 807
BsaXI ACNNNNNCTCC 2 cut(s) 833, 863
Bsc4I CCNNNNNNNGG 3 cut(s) 617, 695, 727
Bse118I RCCGGY 1 cut(s) 1015
Bse1I ACTGG 1 cut(s) 241
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 3 cut(s) 666, 872, 1075
BseDI CCNNGG 2 cut(s) 665, 694
BseGI GGATG 2 cut(s) 343, 816
BseLI CCNNNNNNNGG 3 cut(s) 617, 695, 727
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 3 cut(s) 114, 854, 1112
BseXI GCAGC 1 cut(s) 103
Bsh1285I CGRYCG 2 cut(s) 137, 974
BshFI GGCC 2 cut(s) 720, 731
BshNI GGYRCC 1 cut(s) 698
BsiEI CGRYCG 2 cut(s) 137, 974
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 3 cut(s) 808, 1006, 1016
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 3 cut(s) 617, 695, 727
BsmAI GTCTC 1 cut(s) 520
BsmFI GGGAC 1 cut(s) 1016
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 2 cut(s) 720, 731
BsoBI CYCGRG 1 cut(s) 516
Bsp143I GATC 3 cut(s) 169, 253, 971
BspACI CCGC 2 cut(s) 133, 401
BspANI GGCC 2 cut(s) 720, 731
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 4 cut(s) 700, 777, 1004, 1104
BspT107I GGYRCC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 1015
BsrI ACTGG 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 1015
BssECI CCNNGG 2 cut(s) 665, 694
BssMI GATC 3 cut(s) 169, 253, 971
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 3 cut(s) 666, 872, 1075
Bst4CI ACNGT 1 cut(s) 533
Bst6I CTCTTC 2 cut(s) 321, 533
BstAPI GCANNNNNTGC 1 cut(s) 398
BstBAI YACGTR 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 149, 1017, 1143
BstDEI CTNAG 2 cut(s) 321, 594
BstF5I GGATG 2 cut(s) 343, 816
BstKTI GATC 3 cut(s) 172, 256, 974
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 3 cut(s) 169, 253, 971
BstMCI CGRYCG 2 cut(s) 137, 974
BstMWI GCNNNNNNNGC 3 cut(s) 215, 398, 443
BstNI CCWGG 3 cut(s) 666, 872, 1075
BstNSI RCATGY 4 cut(s) 203, 408, 869, 967
BstSCI CCNGG 4 cut(s) 664, 870, 1005, 1073
BstSNI TACGTA 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 103
Bsu36I CCTNAGG 1 cut(s) 594
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 2 cut(s) 720, 731
BtsCI GGATG 2 cut(s) 343, 816
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 2 cut(s) 248, 855
Cac8I GCNNGC 3 cut(s) 149, 1017, 1143
CaiI CAGNNNCTG 1 cut(s) 446
Cfr10I RCCGGY 1 cut(s) 1015
Cfr13I GGNCC 3 cut(s) 298, 427, 1003
Csp6I GTAC 3 cut(s) 410, 662, 699
CviAII CATG 8 cut(s) 113, 200, 405, 836, 866, 917, 964, 1067
CviQI GTAC 3 cut(s) 410, 662, 699
DdeI CTNAG 2 cut(s) 321, 594
DpnI GATC 3 cut(s) 171, 255, 973
DpnII GATC 3 cut(s) 169, 253, 971
DraI TTTAAA 3 cut(s) 423, 616, 1051
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 321, 533
EarI CTCTTC 2 cut(s) 321, 533
Eco105I TACGTA 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 694
Eco47I GGWCC 3 cut(s) 298, 427, 1003
Eco57I CTGAAG 2 cut(s) 630, 1157
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 1 cut(s) 516
EcoO109I RGGNCCY 2 cut(s) 298, 427
EcoRII CCWGG 3 cut(s) 664, 870, 1073
EcoT14I CCWWGG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 694
FaeI CATG 8 cut(s) 116, 203, 408, 839, 869, 920, 967, 1070
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 1016
FatI CATG 8 cut(s) 112, 199, 404, 835, 865, 916, 963, 1066
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 117, 402
FokI GGATG 2 cut(s) 330, 823
Fsp4HI GCNGC 2 cut(s) 117, 402
FspBI CTAG 2 cut(s) 453, 930
GluI GCNGC 2 cut(s) 117, 402
HaeIII GGCC 2 cut(s) 720, 731
HapII CCGG 3 cut(s) 808, 1006, 1016
Hin1II CATG 8 cut(s) 116, 203, 408, 839, 869, 920, 967, 1070
HincII GTYRAC 1 cut(s) 744
HindII GTYRAC 1 cut(s) 744
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 331, 842, 1070, 1078
HpaII CCGG 3 cut(s) 808, 1006, 1016
HphI GGTGA 2 cut(s) 809, 988
Hpy166II GTNNAC 3 cut(s) 163, 410, 744
Hpy188I TCNGA 3 cut(s) 322, 649, 1137
Hpy188III TCNNGA 3 cut(s) 347, 516, 1082
Hpy8I GTNNAC 3 cut(s) 163, 410, 744
HpyAV CCTTC 1 cut(s) 654
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4IV ACGT 4 cut(s) 222, 412, 823, 958
HpyCH4V TGCA 9 cut(s) 119, 209, 314, 366, 710, 755, 763, 865, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 398, 443
HpyF3I CTNAG 2 cut(s) 321, 594
HpySE526I ACGT 4 cut(s) 222, 412, 823, 958
Hsp92II CATG 8 cut(s) 116, 203, 408, 839, 869, 920, 967, 1070
KpnI GGTACC 1 cut(s) 702
KroI GCCGGC 1 cut(s) 1015
KroNI GCCGGC 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 3 cut(s) 169, 253, 971
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 4 cut(s) 128, 323, 801, 1028
MaeI CTAG 2 cut(s) 453, 930
MaeII ACGT 4 cut(s) 222, 412, 823, 958
MaeIII GTNAC 3 cut(s) 64, 281, 1123
MalI GATC 3 cut(s) 171, 255, 973
MboI GATC 3 cut(s) 169, 253, 971
MboII GAAGA 9 cut(s) 262, 338, 550, 599, 644, 647, 896, 933, 1097
MfeI CAATTG 1 cut(s) 770
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 8 cut(s) 35, 758, 770, 983, 1035, 1046, 1109, 1154
MluNI TGGCCA 1 cut(s) 720
MlyI GAGTC 2 cut(s) 851, 1072
MmeI TCCRAC 2 cut(s) 85, 1053
MnlI CCTC 9 cut(s) 63, 92, 316, 742, 832, 990, 1048, 1090, 1093
Mox20I TGGCCA 1 cut(s) 720
Mph1103I ATGCAT 1 cut(s) 316
MroNI GCCGGC 1 cut(s) 1015
MroXI GAANNNNTTC 1 cut(s) 330
MscI TGGCCA 1 cut(s) 720
MseI TTAA 7 cut(s) 192, 422, 488, 615, 986, 1034, 1050
Msp20I TGGCCA 1 cut(s) 720
MspI CCGG 3 cut(s) 808, 1006, 1016
MspR9I CCNGG 4 cut(s) 666, 872, 1007, 1075
MunI CAATTG 1 cut(s) 770
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 3 cut(s) 666, 872, 1075
MwoI GCNNNNNNNGC 3 cut(s) 215, 398, 443
NaeI GCCGGC 1 cut(s) 1017
NciI CCSGG 1 cut(s) 1007
NdeII GATC 3 cut(s) 169, 253, 971
NgoMIV GCCGGC 1 cut(s) 1015
NlaIII CATG 8 cut(s) 116, 203, 408, 839, 869, 920, 967, 1070
NlaIV GGNNCC 4 cut(s) 700, 777, 1004, 1104
NmuCI GTSAC 2 cut(s) 64, 281
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 4 cut(s) 203, 408, 869, 967
PaeR7I CTCGAG 1 cut(s) 516
PctI GAATGC 1 cut(s) 564
PdiI GCCGGC 1 cut(s) 1017
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 331, 1070
PflMI CCANNNNNTGG 1 cut(s) 727
PfoI TCCNGGA 1 cut(s) 1073
PkrI GCNGC 2 cut(s) 118, 403
Ple19I CGATCG 1 cut(s) 974
PleI GAGTC 2 cut(s) 850, 1072
PpsI GAGTC 2 cut(s) 850, 1072
Ppu21I YACGTR 1 cut(s) 413
PpuMI RGGWCCY 2 cut(s) 298, 427
PshBI ATTAAT 1 cut(s) 986
Psp5II RGGWCCY 2 cut(s) 298, 427
Psp6I CCWGG 3 cut(s) 664, 870, 1073
PspGI CCWGG 3 cut(s) 664, 870, 1073
PspN4I GGNNCC 4 cut(s) 700, 777, 1004, 1104
PspPI GGNCC 3 cut(s) 298, 427, 1003
PspPPI RGGWCCY 2 cut(s) 298, 427
PstNI CAGNNNCTG 1 cut(s) 446
PvuI CGATCG 1 cut(s) 974
RsaI GTAC 3 cut(s) 411, 663, 700
RsaNI GTAC 3 cut(s) 410, 662, 699
SaqAI TTAA 7 cut(s) 192, 422, 488, 615, 986, 1034, 1050
SatI GCNGC 2 cut(s) 117, 402
Sau3AI GATC 3 cut(s) 169, 253, 971
Sau96I GGNCC 3 cut(s) 298, 427, 1003
SchI GAGTC 2 cut(s) 851, 1072
ScrFI CCNGG 4 cut(s) 666, 872, 1007, 1075
SfaNI GCATC 4 cut(s) 128, 323, 801, 1028
Sfr274I CTCGAG 1 cut(s) 516
SinI GGWCC 3 cut(s) 298, 427, 1003
SlaI CTCGAG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
SnaBI TACGTA 1 cut(s) 413
Sse9I AATT 8 cut(s) 35, 758, 770, 983, 1035, 1046, 1109, 1154
SsiI CCGC 2 cut(s) 133, 401
SspI AATATT 1 cut(s) 714
SspMI CTAG 2 cut(s) 453, 930
StyD4I CCNGG 4 cut(s) 664, 870, 1005, 1073
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 1 cut(s) 533
TaiI ACGT 4 cut(s) 225, 415, 826, 961
TaqI TCGA 4 cut(s) 196, 497, 517, 1120
TasI AATT 8 cut(s) 35, 758, 770, 983, 1035, 1046, 1109, 1154
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 2 cut(s) 331, 1070
Tru1I TTAA 7 cut(s) 192, 422, 488, 615, 986, 1034, 1050
Tru9I TTAA 7 cut(s) 192, 422, 488, 615, 986, 1034, 1050
TscAI CASTG 2 cut(s) 248, 862
TseFI GTSAC 2 cut(s) 64, 281
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 2 cut(s) 64, 281
TspDTI ATGAA 5 cut(s) 174, 570, 996, 1077, 1083
TspRI CASTG 2 cut(s) 248, 862
Van91I CCANNNNNTGG 1 cut(s) 727
VpaK11BI GGWCC 3 cut(s) 298, 427, 1003
VspI ATTAAT 1 cut(s) 986
XapI RAATTY 3 cut(s) 1046, 1109, 1154
XceI RCATGY 4 cut(s) 203, 408, 869, 967
XhoI CTCGAG 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 453, 930
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.