FvH4_1g29991

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005059
Physical Location & Seq
Reverse (-)
23738111 .. 23751571
13461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29991.t1

Sequence Viewer

Length: 1434 bp
ATGATGGCGGACAAGCATCGTGCTCTCACATTTCTCGGACTCGGAGCTCTGCTGGGCTCCGTCTCCACCATTCTTCTGCTCAAGCTTCTACCGAGAAGGATTGCAAGCCGTTGTGATAAAAAGGCTCTCCAAATGCTTGATATTAAGGGTAATGGGTTGGCATCTGAGACACCTACGAATTGCAGTGCAGTTACTGGGAGTGGAAATAGCAAGTTAGCTGGTGTAGACCTTCTAAATGATGAGATAGTCTCTGAACAACTAACAAGGAACATTCAATTCTTTGGAGTTGAATCTCAACAAAAAGTGACTGCTTCCTATGTTGTGGTCATTGGTCTCGGAGGAGTTGGAAGTCATGCTGCATCAATGCTTTTGCGGTCGGGGGTTGGCAGGCTTCTCCTCGTGGACTTTGATCAGGTATCAGTCTCATCACTTAATCGACATGCTGTTGCAACAAGAGCTGACGTTGGTATCCCAAAAGCCCAGTGTCTAAAAGATCATTTCTTGTCTATCTTCCCAGAGTGTCACATAGAAGCAAAGGTCCTGTTATACAATGTATCGTCTGAGGAAGAGATTCTTTCCGGTCATCCTGATTTTGTTTTGGACTGCATCGACAACATTGATACAAAGGTGGCTCTTCTTGCGGCATGTGTACATAGGGGTTTAAAGATCCTATCAGCCACAGGAGCTGGTGCTAGAGCTGACCCGACAAGAATACGCATCGCTGATTTAAGACAGTCAACAAATGACCCATTATCTCGAGCTGTGAGACACCGTTTAAAGAGAGAGTATGGCATTGAAGGTGGCATACCTGTTGTCTTCTCATTGGAGAAACCTAAGGCAAAGCTTCTTCCATTCAAAGGACCAAGTGGAGAAGAAGAAAATCCTTCAGATTACCAGATACTACCAGGGTTTAGGGTTCGCATAATACCTGTCCTTGGTACCATTCCTGCAATATTTGGACAAGTCATGGCCTCCTATGTGTTAACACAACTTGCAGAATTGCAAGTTCAACCGGAACCTGTGATAAACTTGGACATTGACCATTACAGAATGCTTCACCAACGCCTTATTGAGCATGAGGAATCATTGTATGGCACTGCTATGCATGTCCAGGTAGATGTTGAAGAAGTGATGTATGTTGCAAAAGAATTATGGCATGGAAGAAGTGCTAGAGAGCCATCGGCTAAAGATGTAGGACGTAGCATGTGGCGATCGGTGAATGAATTAATGCTTGTGAGGTGGGATCGGGAAAAGCCAGCATCTGTGTTCAATTTAATTCTCTTGAAATTTAAAGAGCAACTGTTGCCAAAAAGAAAGAATAGAAAACCATCAATGCTATTGTTCTTTCCCGGTGGTGCATTTGTTGAGTCGATGACATCTTCTGTGACAATTCAAGTGCAGATGAACATGAATCCAGCAGTCTTGAAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

478

Amino Acids

52.61

Weight (kDa)

8.88

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 88 - 334 1.1e-44 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 938
AccI GTMKAC 1 cut(s) 225
AciI CCGC 3 cut(s) 8, 373, 641
AclWI GGATC 2 cut(s) 661, 1251
AcsI RAATTY 1 cut(s) 1286
AcuI CTGAAG 1 cut(s) 870
AfaI GTAC 2 cut(s) 651, 940
AfiI CCNNNNNNNGG 2 cut(s) 857, 935
AjnI CCWGG 2 cut(s) 904, 1110
AluBI AGCT 8 cut(s) 47, 85, 218, 458, 686, 698, 761, 844
AluI AGCT 8 cut(s) 47, 85, 218, 458, 686, 698, 761, 844
Alw21I GWGCWC 2 cut(s) 25, 49
Alw26I GTCTC 6 cut(s) 67, 161, 253, 338, 427, 760
AlwI GGATC 2 cut(s) 661, 1251
AlwNI CAGNNNCTG 3 cut(s) 194, 686, 1262
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 1 cut(s) 969
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 1286
AseI ATTAAT 1 cut(s) 1226
Asp700I GAANNNNTTC 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 938
AspS9I GGNCC 2 cut(s) 538, 860
AsuC2I CCSGG 1 cut(s) 1350
AsuHPI GGTGA 2 cut(s) 1049, 1228
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 2 cut(s) 538, 860
AxyI CCTNAGG 1 cut(s) 834
BaeI ACNNNNGTAYC 4 cut(s) 451, 484, 612, 645
BanI GGYRCC 1 cut(s) 938
BanII GRGCYC 2 cut(s) 49, 59
BarI GAAGNNNNNNTAC 2 cut(s) 789, 821
BauI CACGAG 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 808
Bbv12I GWGCWC 2 cut(s) 25, 49
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 1186, 1336
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 2 cut(s) 906, 1112
BciVI GTATCC 1 cut(s) 479
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 1350
BcoDI GTCTC 6 cut(s) 67, 161, 253, 338, 427, 760
BfaI CTAG 2 cut(s) 693, 1170
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 2 cut(s) 357, 642
BlsI GCNGC 2 cut(s) 358, 643
Bme1390I CCNGG 3 cut(s) 906, 1112, 1350
Bme18I GGWCC 2 cut(s) 538, 860
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 2 cut(s) 538, 860
BmiI GGNNCC 3 cut(s) 58, 940, 1017
BmrFI CCNGG 3 cut(s) 906, 1112, 1350
BmrI ACTGGG 2 cut(s) 204, 475
BmsI GCATC 6 cut(s) 25, 170, 368, 615, 726, 1268
BmuI ACTGGG 2 cut(s) 204, 475
BpiI GAAGAC 1 cut(s) 808
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 1350
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 905, 934
BsaWI WCCGGW 2 cut(s) 578, 1012
Bsc4I CCNNNNNNNGG 2 cut(s) 857, 935
Bse1I ACTGG 2 cut(s) 199, 481
Bse21I CCTNAGG 1 cut(s) 834
BseBI CCWGG 2 cut(s) 906, 1112
BseDI CCNNGG 2 cut(s) 905, 934
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 2 cut(s) 857, 935
BseMII CTCAG 2 cut(s) 156, 552
BseNI ACTGG 2 cut(s) 199, 481
BseRI GAGGAG 2 cut(s) 354, 386
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 207, 1418
Bsh1285I CGRYCG 2 cut(s) 377, 1214
BshFI GGCC 1 cut(s) 971
BshNI GGYRCC 1 cut(s) 938
BsiEI CGRYCG 2 cut(s) 377, 1214
BsiHKAI GWGCWC 2 cut(s) 25, 49
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 3 cut(s) 579, 1013, 1350
BslI CCNNNNNNNGG 2 cut(s) 857, 935
BsmAI GTCTC 6 cut(s) 67, 161, 253, 338, 427, 760
BsmBI CGTCTC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 1056
BsnI GGCC 1 cut(s) 971
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 756
Bsp1286I GDGCHC 3 cut(s) 25, 49, 59
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 5 cut(s) 409, 493, 666, 1211, 1243
BspACI CCGC 3 cut(s) 8, 373, 641
BspANI GGCC 1 cut(s) 971
BspCNI CTCAG 2 cut(s) 157, 553
BspLI GGNNCC 3 cut(s) 58, 940, 1017
BspPI GGATC 2 cut(s) 661, 1251
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 938
BspTNI GGTCTC 1 cut(s) 338
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 2 cut(s) 199, 481
BssECI CCNNGG 2 cut(s) 905, 934
BssMI GATC 5 cut(s) 409, 493, 666, 1211, 1243
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 934
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 906, 1112
Bst4CI ACNGT 3 cut(s) 735, 773, 1302
Bst6I CTCTTC 2 cut(s) 561, 639
BstAPI GCANNNNNTGC 1 cut(s) 1303
BstAUI TGTACA 1 cut(s) 649
BstC8I GCNNGC 3 cut(s) 106, 389, 1257
BstDEI CTNAG 3 cut(s) 165, 561, 834
BstF5I GGATG 1 cut(s) 583
BstKTI GATC 5 cut(s) 412, 496, 669, 1214, 1246
BstMAI GTCTC 6 cut(s) 67, 161, 253, 338, 427, 760
BstMBI GATC 5 cut(s) 409, 493, 666, 1211, 1243
BstMCI CGRYCG 2 cut(s) 377, 1214
BstMWI GCNNNNNNNGC 4 cut(s) 455, 638, 683, 1303
BstNI CCWGG 2 cut(s) 906, 1112
BstNSI RCATGY 4 cut(s) 443, 648, 1109, 1207
BstSCI CCNGG 3 cut(s) 904, 1110, 1348
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 808
BstX2I RGATCY 1 cut(s) 666
BstYI RGATCY 1 cut(s) 666
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 1 cut(s) 971
BtgZI GCGATG 1 cut(s) 703
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 2 cut(s) 190, 1095
BtsIMutI CAGTG 3 cut(s) 190, 488, 1095
Cac8I GCNNGC 3 cut(s) 106, 389, 1257
CaiI CAGNNNCTG 3 cut(s) 194, 686, 1262
Cfr13I GGNCC 2 cut(s) 538, 860
Csp6I GTAC 2 cut(s) 650, 939
CviAII CATG 9 cut(s) 353, 440, 645, 967, 1076, 1106, 1157, 1204, 1408
CviQI GTAC 2 cut(s) 650, 939
DdeI CTNAG 3 cut(s) 165, 561, 834
DpnI GATC 5 cut(s) 411, 495, 668, 1213, 1245
DpnII GATC 5 cut(s) 409, 493, 666, 1211, 1243
DraI TTTAAA 3 cut(s) 663, 777, 1291
Eam1104I CTCTTC 2 cut(s) 561, 639
EarI CTCTTC 2 cut(s) 561, 639
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 934
Eco24I GRGCYC 2 cut(s) 49, 59
Eco31I GGTCTC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 538, 860
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 870
Eco81I CCTNAGG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 538
EcoRII CCWGG 2 cut(s) 904, 1110
EcoT14I CCWWGG 1 cut(s) 934
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 2 cut(s) 49, 59
ErhI CCWWGG 1 cut(s) 934
Esp3I CGTCTC 1 cut(s) 67
FaeI CATG 9 cut(s) 356, 443, 648, 970, 1079, 1109, 1160, 1207, 1411
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FatI CATG 9 cut(s) 352, 439, 644, 966, 1075, 1105, 1156, 1203, 1407
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 357, 642
FokI GGATG 1 cut(s) 570
FriOI GRGCYC 2 cut(s) 49, 59
Fsp4HI GCNGC 2 cut(s) 357, 642
FspBI CTAG 2 cut(s) 693, 1170
GluI GCNGC 2 cut(s) 357, 642
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 1 cut(s) 971
HapII CCGG 3 cut(s) 579, 1013, 1350
Hin1II CATG 9 cut(s) 356, 443, 648, 970, 1079, 1109, 1160, 1207, 1411
HincII GTYRAC 2 cut(s) 738, 984
HindII GTYRAC 2 cut(s) 738, 984
HindIII AAGCTT 2 cut(s) 83, 842
HinfI GANTC 6 cut(s) 39, 290, 571, 1082, 1367, 1411
HpaI GTTAAC 1 cut(s) 984
HpaII CCGG 3 cut(s) 579, 1013, 1350
HphI GGTGA 2 cut(s) 1049, 1228
Hpy166II GTNNAC 5 cut(s) 226, 403, 650, 738, 984
Hpy188I TCNGA 7 cut(s) 38, 44, 166, 253, 338, 562, 889
Hpy188III TCNNGA 5 cut(s) 587, 756, 1247, 1282, 1423
Hpy8I GTNNAC 5 cut(s) 226, 403, 650, 738, 984
HpyAV CCTTC 4 cut(s) 90, 239, 791, 894
HpyCH4III ACNGT 3 cut(s) 735, 773, 1302
HpyCH4IV ACGT 2 cut(s) 462, 1198
HpyF10VI GCNNNNNNNGC 4 cut(s) 455, 638, 683, 1303
HpyF3I CTNAG 3 cut(s) 165, 561, 834
HpySE526I ACGT 2 cut(s) 462, 1198
Hsp92II CATG 9 cut(s) 356, 443, 648, 970, 1079, 1109, 1160, 1207, 1411
KpnI GGTACC 1 cut(s) 942
Ksp22I TGATCA 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 984
Kzo9I GATC 5 cut(s) 409, 493, 666, 1211, 1243
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 44, 62, 683
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 6 cut(s) 25, 170, 368, 615, 726, 1268
MaeI CTAG 2 cut(s) 693, 1170
MaeII ACGT 2 cut(s) 462, 1198
MaeIII GTNAC 4 cut(s) 190, 304, 521, 1384
MalI GATC 5 cut(s) 411, 495, 668, 1213, 1245
MboI GATC 5 cut(s) 409, 493, 666, 1211, 1243
MflI RGATCY 1 cut(s) 666
MhlI GDGCHC 3 cut(s) 25, 49, 59
MluCI AATT 9 cut(s) 178, 275, 998, 1148, 1223, 1270, 1275, 1286, 1389
MlyI GAGTC 2 cut(s) 33, 1376
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 6 cut(s) 332, 407, 556, 982, 1072, 1230
Mph1103I ATGCAT 1 cut(s) 1107
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 9 cut(s) 144, 432, 662, 728, 776, 983, 1226, 1274, 1290
MslI CAYNNNNRTG 1 cut(s) 1100
MspI CCGG 3 cut(s) 579, 1013, 1350
MspR9I CCNGG 3 cut(s) 906, 1112, 1350
Mva1269I GAATGC 1 cut(s) 1056
MvaI CCWGG 2 cut(s) 906, 1112
MwoI GCNNNNNNNGC 4 cut(s) 455, 638, 683, 1303
NciI CCSGG 1 cut(s) 1350
NdeII GATC 5 cut(s) 409, 493, 666, 1211, 1243
NlaIII CATG 9 cut(s) 356, 443, 648, 970, 1079, 1109, 1160, 1207, 1411
NlaIV GGNNCC 3 cut(s) 58, 940, 1017
NmuCI GTSAC 3 cut(s) 304, 521, 1384
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 4 cut(s) 443, 648, 1109, 1207
PaeR7I CTCGAG 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 1056
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 4 cut(s) 290, 571, 1082, 1411
PkrI GCNGC 2 cut(s) 358, 643
Ple19I CGATCG 1 cut(s) 1214
PleI GAGTC 2 cut(s) 33, 1375
PpsI GAGTC 2 cut(s) 33, 1375
PpuMI RGGWCCY 1 cut(s) 538
PshBI ATTAAT 1 cut(s) 1226
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 538
Psp6I CCWGG 2 cut(s) 904, 1110
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 904, 1110
PspN4I GGNNCC 3 cut(s) 58, 940, 1017
PspPI GGNCC 2 cut(s) 538, 860
PspPPI RGGWCCY 1 cut(s) 538
PstNI CAGNNNCTG 3 cut(s) 194, 686, 1262
PsuI RGATCY 1 cut(s) 666
PvuI CGATCG 1 cut(s) 1214
RsaI GTAC 2 cut(s) 651, 940
RsaNI GTAC 2 cut(s) 650, 939
RseI CAYNNNNRTG 1 cut(s) 1100
SacI GAGCTC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 9 cut(s) 144, 432, 662, 728, 776, 983, 1226, 1274, 1290
SatI GCNGC 2 cut(s) 357, 642
Sau3AI GATC 5 cut(s) 409, 493, 666, 1211, 1243
Sau96I GGNCC 2 cut(s) 538, 860
SchI GAGTC 2 cut(s) 33, 1376
ScrFI CCNGG 3 cut(s) 906, 1112, 1350
SduI GDGCHC 3 cut(s) 25, 49, 59
SfaNI GCATC 6 cut(s) 25, 170, 368, 615, 726, 1268
Sfr274I CTCGAG 1 cut(s) 756
SinI GGWCC 2 cut(s) 538, 860
SlaI CTCGAG 1 cut(s) 756
SmiMI CAYNNNNRTG 1 cut(s) 1100
SmlI CTYRAG 2 cut(s) 80, 756
SmoI CTYRAG 2 cut(s) 80, 756
Sse9I AATT 9 cut(s) 178, 275, 998, 1148, 1223, 1270, 1275, 1286, 1389
SsiI CCGC 3 cut(s) 8, 373, 641
SspI AATATT 1 cut(s) 954
SspMI CTAG 2 cut(s) 693, 1170
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 3 cut(s) 904, 1110, 1348
StyI CCWWGG 1 cut(s) 934
TaaI ACNGT 3 cut(s) 735, 773, 1302
TaiI ACGT 2 cut(s) 465, 1201
TaqI TCGA 4 cut(s) 436, 609, 757, 1370
TasI AATT 9 cut(s) 178, 275, 998, 1148, 1223, 1270, 1275, 1286, 1389
TatI WGTACW 1 cut(s) 649
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 4 cut(s) 290, 571, 1082, 1411
Tru1I TTAA 9 cut(s) 144, 432, 662, 728, 776, 983, 1226, 1274, 1290
Tru9I TTAA 9 cut(s) 144, 432, 662, 728, 776, 983, 1226, 1274, 1290
TscAI CASTG 3 cut(s) 190, 488, 1102
TseFI GTSAC 3 cut(s) 304, 521, 1384
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 3 cut(s) 304, 521, 1384
TspDTI ATGAA 3 cut(s) 1236, 1418, 1424
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 3 cut(s) 190, 488, 1102
VpaK11BI GGWCC 2 cut(s) 538, 860
VspI ATTAAT 1 cut(s) 1226
XapI RAATTY 1 cut(s) 1286
XceI RCATGY 4 cut(s) 443, 648, 1109, 1207
XhoI CTCGAG 1 cut(s) 756
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 2 cut(s) 693, 1170
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.