AT5G42950

GYF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008668
Physical Location & Seq
Forward (+)
17224270 .. 17231384
7115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42950.1

Sequence Viewer

Length: 5145 bp
ATGGCTAACTCTTCCGCTGGCTCCGCCGCAGACCACCGCAACAAACACCTCTCCGTCAATCCACCGCACCAGATCTTCAAAGATATCCAAGGTTCTGACAATGCGATTCCTCTTTCACCACAGTGGCTTCTCTCCAAACCAGGGGAGAACAAGACTGGGATGGGAACTGGGGATCCTAATCAGTATGGAAACCATTCGGATGTTGTGAGAACAACAGGGAATGGGGAGGAGACACTGGATAATCTGAAGAAAAAAGATGTTTTCCGGCCATCCTTGCTTGATGCAGAAAGTGGTCGTCGTGATCGTTGGCGTGATGAGGAAAGGGACACCTTGTCCTCAGTCCGAAATGACCGCTGGCGGAATGGCGACAAAGACTCTGGTGATAATAAGAAGGTTGACCGGTGGGATAATGTGGCTCCTAAATTTGGGGAACAACGACGTGGTCCGAATGACCGGTGGACTGATTCAGGAAACAAGGATGCTGCGCCAGAGCAGAGGCGTGAGAGCAAGTGGAACTCTCGCTGGGGTCCTGATGACAAGGAAGCTGAGATTCCGCGTAATAAGTGGGATGAACCTGGTAAGGACGGTGAAATCATTCGTGAGAAGGGTCCATCTCTTCCTACTAGCGATGGAGACCATTACCGGCCCTGGAGACCCTCTCAAGGTCGAGGAAGAGGAGAAGCTCTTCACAACCAATCAACACCAAACAAACAGGTTACTTCCTTCTCCCACAGCAGGGGGCGTGGAGAGAACACTGCTATCTTTTCAGCTGGACGTGGAAGGATGAGTCCTGGTGGAAGCATTTTTACTAGCGCACCAAACCAGTCTCATCCCCCTGGATCTGCCTCTGACAAGGGGGAAAGTGGTCCTGGAGAACCTCCCCATCTGAGATATAGCAGAATGAAACTGTTGGATGTGTACAGGATGGCTGACACAGAGTGTTATGAAAAGTTTCCGGATGGGTTTATTGAGGTGCCTTCCCTAACGTCTGAGGAGCCAACGGATCCTCTGGCTCTTTGTGCTCCAAGTTCCGATGAAGTGAATGTTCTGGATGCGATTGAGAAAGGAAAAATAGTGAGCAGTGGTGCCCCTCAGACGTCCAAGGATGGCCCTACTGGACGAAATCCGGTCGAGTTTTCACAACCTAGACGGATCAGGCCTGCTGGAAGCAGAGAAGATATGACATTCGGTGCTGAGGAGTCTAAAGATGAAAGTGGAGAAACAAGGAACTATCCAGATGATAAGTTTAGGCCTGAAGCTTCTCATGAAGGTTATGCACCTTTTAGGAGAGGCAATGAGGCACCTGTCAGAGAACTGAAAGAACCCAGTATGCAGGGAAATGCTCATGTTCAATCTGCCTCTCCATGGCGTCAGTCTTCTGGGGGAGAAAGGTCGAATAGGAACTCACATGATTGGAATGACCCTTCAGCTGATAGCAGGCTGAAATCTTCTGACAGTGTTTGGTCGCATCCTAAAGATTCAATAAATCATTTAGGTGGCAATAATATGATGTTGCCGCAGTCGAAAGGTGAATCAAGATGGCAAATCAGTGAAGATCCTTCACTTAGAAGGCAGCCATCTCTGGTGTTCGACAGGGAGCAGGAAGTTAGAAAGCTTCTCCCATCTTCGCCTGAAGAACTTTCACTCTATTATAAAGATCCTCAGGGTCTAATTCAAGGCCCTTTTTCTGGATCTGATATCATTGGATGGTTTGAGGCTGGGTATTTTGGCATAGATTTGCTAGTTCGTCTTGCAAGTGCACCAAATGATTCTCCTTTTTCATTACTTGGTGATGTAATGCCACATTTACGGGCTAAGTCGGGTCCACCACCTGGTTTTACTGGTGCCAAGCAAAACGAATTTGTTGATGCAGCTGGTACATCAGCCTTCCCTGGTGTGGGGAAAGTTCATTCTGGGATGGGTGAGACTGATATGTTGCAAAATGATATGAGGTATAAGCATGTTGCAGGAACTGTAGCGGAGAACCGGTTTATTGAATCATTGATGTCTGGGGGTCTGACCAATTCAGCTCAAGGTGTTCAAGGATATGGAGTAAATAGTTCTGGTGGGTTGTCTTTACCAGTTACTGATGGTGGGGCTGATATGTATCTCCTGGCCAAGAAATTGGAACTTGAGCGGCAGAGATCAATACCTAGTCCGTATTCATATTGGCCTGGTCGGGAATCTGCAAACCTGATGCCAGGATCAGAGAATGTGTCAGAAAATGCTCAACAACCTACCCGTTCTCCAAGTTCTGATTTGTTGTCCATCCTCCAAGGTGTAACGGATAGGTCTTCTCCTGCTGTTAGTGGTCCTCTTCCTGCTTGGTCTCAACCCATTCAAAAGGAAAGTGATTTGCACCATGCTAAAACTTTCCAAACGCAAATTCCCTTTGGGGTCCAACAGCAGAGACTGCCAGAGCAGAATTTACCTTTGTCAGGTTTACTTGGTCAACCTATGGAAAATAATCCAGGTGGCATGTTATCTCCTGATATGATGCTCGCTGCTGGACTCTCTCAAGAGCATCAATCGCTCAATCTGTTGCAGCAGCAACAGCTCTTGTTGCAGTTGAATGCTCAGACACCACTTTCTGCCCAACATCAGCGTCTATTGGTGGAAAAGATGCTCTTGCTTAAACACCAACATAAACAAGAAGAGCAGCAGCAATTGTTACGACAGCAACAGCAGCTGTATTCTCAGGTTTTTGCTGATCAACAGCGTTCTCAGCAACGGTTTGGAGACCCATCTTATGGTCAGTTGCAGGCGTCTCTTGATGCGCTTAGGCTGCAACCATCAAAAGATATGTCACAAGTCAATCAGCAGGTGCAGGTTCCTGTTTCCCATGAGGAGCGAGGCATCAACTTAGCTGATTTGCTCCCAGTAACCCATGCCACTAATCAGACTGTTGCTTCTTTCGAAACCCCCTCTCTGCACCTGCAAAACCAGCTATTTGGTAATGTTGACCCTAGGATGGTTCTGCCTGATCAAATTGATGATACCCATAAAAAAGAGTCGAAATCAGAATATGAAAGAACAGTTTCTGCAGACTATGTGAACAGCTTGTACTCAGAAAAGCCTGTCCTTTCCCCTGGCTATCATGCTACACATAATGTGGAAGAACCTGTGAGCTATCCAAACAATGAAAGCTCCACTGCTACTATGACAGCTCCCGAAATAGTTGAAAGTAAGTTGCTGGAGGAGCAGTCTAAGGACATGTATGCTGGAAAGGGAGAGGTCAGTATTGAGTTATCTGGGGAGACTCCTGCAACTGAAGTTAAAAACAATGACGTTTCTGTAGCACGGAAGACTTCTGAGAAGAAGTCCAGGAAGCAGCGGGCTAAGCAGGCTGCTGACCTGGCTAAGTCAACTTCTAGAGCTCCTCTGCAGGAGACAAAGAAACCTCAACCAGGAAGTGCTGATGATTCTGAGATAAAGGGTAAAACTAAAAAGTCGGCTGACACTTTGATAGACAATGATACTCACCTCATTAAAAGCTCCACAGCCACAGCTTCAAACACTTCCCAAATGAGTTCTGAAGTAGATTCAGTCAGGGGAGAAGAGTCTTCACTGCAAAACACACGAACACAACCAGGACGAGCTTGGAAGCCTGCTCCTGGCTTTAAGCCGAAGTCATTACTGGAAATTCAAATGGAAGAACAGAGGGTAGCGCAAGCAGAAGCTTTAGCTCCAAAGATTTCTTCTACCGTGAATTCAGTGGGTTCGGCGGCTCCTTGGGCTGGAATTGTTACCAATTCAGATTCTAACATACTAAGGGAGACTCATGGAGAGTCAGCTATTACTCAAACCGGTGTTGTAAAGCCTGAAAGTGTTCCTACTCTTAAGGCTAAGAAAAGCCACTTGCATGACTTGCTGGCTGATGATGTTTTTGCCAAATCCAGTGACAAAGAGAGGGAAGTAATGGAAATCATCTCTAACAATGATGCATTTATGCAAGTCACGACCACTAATGCAGAGTCTTTCGACGATGATAACTTTATTGACGCTAGGGAAACAAAAAAGAGCCGTAAGAAATCTGCGAGGGCAAAAACTTCTGGTGCAAAAATTGCTGCACATGTTCCTGCTGTAGATACATCCCTCCAAACAAACTCTGTTGAGAAGGGAAAAAGTTCTCGTATTCTGCAGCAGCAGGAGAAGGAAGTTTTGCCGGCTATTCCTTCTGGGCCTTCTCTGGGAGATTTTGTTCTCTGGAAAGGAGAGTCTGTAAACAATCCTCCACCGGCTGCTGCATGGTCTTCTGGTCCCAAAAAATCTACAAAACCTAGCTCGCTTAGGGACATCGTGAAGGAGCAGGAGAAGATGACGACCTCATCTCATCCACCCCCTAGTCCAGTACCTACCACCCAGAAAGCTATTCCACCTCAGGCTCATCAGGGCGGTGCTTCCTGGTCGCGTTCTGCATCTTCACCATCGCAGGCTGTTTCTCAATCTTCCTCTCAGTCAAAATCCAAAGGAGATGATGACCTTTTCTGGGGTCCAGTTGAGCAATCAACCCAGGACACAAAACAGGGAGACTTTCCACATCTTACAAGTCAAAACAGTTGGGGAACCAAAAACACTCCTGGAAAAGTTAATGCAGGAACTTCACTGAATCGACAGAAATCAGTTTCAATGGGATCTGCGGATCGGGTTTTGTCTTCACCTGTTGTCACTCAGGCGTCACACAAAGGGAAAAAAGAGGCAGTAACAAAACTCACAGAGGCGAATGGCTTCAGAGATTGGTGCAAAAGCGAATGTCTCAGACTTCTTGGTTCCGAAGATACAAGTGTCTTGGAATTTTGTCTGAAGCTATCCCGATCAGAAGCTGAAACTCTTCTGATAGAAAATCTGGGGTCTCGTGACCCTGACCACAAGTTCATCGACAAATTTCTCAACTACAAAGACCTGTTACCATCAGAAGTAGTTGAGATTGCATTTCAATCAAAGGGCTCGGGAGTCGGGACCCGAAACAACACAGGTGAAGACTATTACTATAACACTACAGCTGCAAATGACGGGTTCTCGAAAGTTGGAGGAAAGAAAAAGGCGAAGAAAGGGAAGAAGGTTAGCTTGAGCGCATCGGTTCTGGGATTTAATGTGGTTAGTAACCGGATCATGATGGGAGAGATTCAGACAATTGAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1714

Amino Acids

187.62

Weight (kDa)

6.29

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 550 - 587 2.4e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1653
AarI CACCTGC 2 cut(s) 2812, 2943
AatII GACGTC 1 cut(s) 1100
Acc36I ACCTGC 3 cut(s) 2812, 2818, 2943
AccB1I GGYRCC 4 cut(s) 973, 1085, 1300, 1844
AccB7I CCANNNNNTGG 2 cut(s) 1832, 2750
AccBSI CCGCTC 1 cut(s) 2137
AccII CGCG 2 cut(s) 556, 4411
AccIII TCCGGA 1 cut(s) 955
AcoI YGGCCR 2 cut(s) 266, 2115
AcsI RAATTY 8 cut(s) 422, 1859, 2385, 2425, 3642, 3709, 4793, 4883
AcuI CTGAAG 8 cut(s) 266, 1275, 1410, 1653, 3291, 3555, 4714, 4823
AcyI GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
AdeI CACNNNGTG 1 cut(s) 939
AfaI GTAC 4 cut(s) 920, 1879, 3065, 4353
AflII CTTAAG 1 cut(s) 3839
AflIII ACRYGT 2 cut(s) 3213, 4072
AgeI ACCGGT 4 cut(s) 399, 453, 1986, 3806
AhdI GACNNNNNGTC 1 cut(s) 331
AjiI CACGTC 2 cut(s) 440, 776
AjuI GAANNNNNNNTTGG 4 cut(s) 2370, 2402, 3682, 3714
AleI CACNNNNGTG 1 cut(s) 121
AloI GAACNNNNNNTCC 4 cut(s) 157, 189, 4185, 4217
Alw21I GWGCWC 3 cut(s) 1024, 1762, 3379
Alw44I GTGCAC 1 cut(s) 1758
AlwNI CAGNNNCTG 6 cut(s) 1973, 2087, 2413, 2689, 3041, 4823
Ama87I CYCGRG 1 cut(s) 4948
Aor13HI TCCGGA 1 cut(s) 955
AoxI GGCC 9 cut(s) 266, 644, 1108, 1157, 1250, 1678, 2115, 2171, 4181
ApaLI GTGCAC 1 cut(s) 1758
ApoI RAATTY 8 cut(s) 422, 1859, 2385, 2425, 3642, 3709, 4793, 4883
ArsI GACNNNNNNTTYG 2 cut(s) 2789, 2821
AsiGI ACCGGT 4 cut(s) 399, 453, 1986, 3806
Asp700I GAANNNNTTC 9 cut(s) 193, 594, 684, 951, 3037, 3828, 4126, 4727, 4830
AspA2I CCTAGG 1 cut(s) 2966
AspLEI GCGC 5 cut(s) 487, 815, 2779, 3670, 5075
AsuII TTCGAA 1 cut(s) 2916
AvaI CYCGRG 1 cut(s) 4948
AvrII CCTAGG 1 cut(s) 2966
AxyI CCTNAGG 2 cut(s) 1662, 4380
BaeGI GKGCMC 2 cut(s) 1090, 1762
BaeI ACNNNNGTAYC 2 cut(s) 4770, 4803
BalI TGGCCA 1 cut(s) 2117
BamHI GGATCC 2 cut(s) 172, 1003
BanI GGYRCC 4 cut(s) 973, 1085, 1300, 1844
BanII GRGCYC 2 cut(s) 3379, 4949
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
BauI CACGAG 1 cut(s) 4854
BbsI GAAGAC 7 cut(s) 1368, 2286, 3311, 3555, 4245, 4647, 4986
Bbv12I GWGCWC 3 cut(s) 1024, 1762, 3379
BbvCI CCTCAGC 1 cut(s) 1194
BceAI ACGGC 1 cut(s) 4008
BclI TGATCA 2 cut(s) 2710, 2983
BfmI CTRYAG 7 cut(s) 1974, 3042, 3294, 3383, 4083, 4139, 4998
BfrI CTTAAG 1 cut(s) 3839
BfuAI ACCTGC 3 cut(s) 2812, 2818, 2943
BglII AGATCT 1 cut(s) 72
BlnI CCTAGG 1 cut(s) 2966
BlpI GCTNAGC 1 cut(s) 3339
BmeRI GACNNNNNGTC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 4948
BmgBI CACGTC 2 cut(s) 440, 776
BmrI ACTGGG 4 cut(s) 165, 177, 1320, 2873
BmuI ACTGGG 4 cut(s) 165, 177, 1320, 2873
BpiI GAAGAC 7 cut(s) 1368, 2286, 3311, 3555, 4245, 4647, 4986
BplI GAGNNNNNCTC 2 cut(s) 643, 675
BpmI CTGGAG 3 cut(s) 670, 891, 3215
Bpu10I CCTNAGC 3 cut(s) 1194, 2780, 4289
Bpu1102I GCTNAGC 1 cut(s) 3339
Bpu14I TTCGAA 1 cut(s) 2916
BpuEI CTTGAG 5 cut(s) 645, 2016, 2153, 2502, 5089
BsaBI GATNNNNATC 2 cut(s) 177, 2106
BsaHI GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
BsaI GGTCTC 5 cut(s) 627, 646, 2334, 2733, 4857
BsaWI WCCGGW 7 cut(s) 399, 453, 955, 1126, 1986, 3806, 5106
Bse118I RCCGGY 7 cut(s) 399, 453, 642, 1986, 3806, 4165, 4237
Bse21I CCTNAGG 2 cut(s) 1662, 4380
Bse3DI GCAATG 1 cut(s) 1300
Bse8I GATNNNNATC 2 cut(s) 177, 2106
BseAI TCCGGA 1 cut(s) 955
BseJI GATNNNNATC 2 cut(s) 177, 2106
BseMI GCAATG 1 cut(s) 1300
BseRI GAGGAG 7 cut(s) 242, 690, 1007, 1211, 2861, 3212, 3369
BseSI GKGCMC 2 cut(s) 1090, 1762
BseYI CCCAGC 2 cut(s) 522, 1718
BsgI GTGCAG 3 cut(s) 2846, 2915, 4053
Bsh1236I CGCG 2 cut(s) 556, 4411
Bsh1285I CGRYCG 1 cut(s) 1131
BshFI GGCC 9 cut(s) 268, 646, 1110, 1159, 1252, 1680, 2117, 2173, 4183
BshNI GGYRCC 4 cut(s) 973, 1085, 1300, 1844
BshTI ACCGGT 4 cut(s) 399, 453, 1986, 3806
BsiEI CGRYCG 1 cut(s) 1131
BsiHKAI GWGCWC 3 cut(s) 1024, 1762, 3379
BsiHKCI CYCGRG 1 cut(s) 4948
BslFI GGGAC 4 cut(s) 338, 4245, 4307, 4972
BsmBI CGTCTC 1 cut(s) 2772
BsmFI GGGAC 4 cut(s) 338, 4245, 4307, 4972
BsmI GAATGC 1 cut(s) 2578
BsnI GGCC 9 cut(s) 268, 646, 1110, 1159, 1252, 1680, 2117, 2173, 4183
Bso31I GGTCTC 5 cut(s) 627, 646, 2334, 2733, 4857
BsoBI CYCGRG 1 cut(s) 4948
Bsp119I TTCGAA 1 cut(s) 2916
Bsp1286I GDGCHC 5 cut(s) 1024, 1090, 1762, 3379, 4949
Bsp13I TCCGGA 1 cut(s) 955
Bsp1407I TGTACA 1 cut(s) 918
Bsp1720I GCTNAGC 1 cut(s) 3339
Bsp19I CCATGG 1 cut(s) 1364
BspANI GGCC 9 cut(s) 268, 646, 1110, 1159, 1252, 1680, 2117, 2173, 4183
BspEI TCCGGA 1 cut(s) 955
BspFNI CGCG 2 cut(s) 556, 4411
BspHI TCATGA 2 cut(s) 1264, 5112
BspMAI CTGCAG 3 cut(s) 3046, 3387, 4143
BspMI ACCTGC 3 cut(s) 2812, 2818, 2943
BspQI GCTCTTC 2 cut(s) 690, 2649
BspT104I TTCGAA 1 cut(s) 2916
BspT107I GGYRCC 4 cut(s) 973, 1085, 1300, 1844
BspTI CTTAAG 1 cut(s) 3839
BspTNI GGTCTC 5 cut(s) 627, 646, 2334, 2733, 4857
BsrBI CCGCTC 1 cut(s) 2137
BsrDI GCAATG 1 cut(s) 1300
BsrFI RCCGGY 7 cut(s) 399, 453, 642, 1986, 3806, 4165, 4237
BsrGI TGTACA 1 cut(s) 918
BssAI RCCGGY 7 cut(s) 399, 453, 642, 1986, 3806, 4165, 4237
BssNI GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
BssSI CACGAG 1 cut(s) 4854
BssT1I CCWWGG 6 cut(s) 88, 1101, 1364, 2275, 2966, 3731
Bst2BI CACGAG 1 cut(s) 4854
Bst6I CTCTTC 8 cut(s) 16, 621, 667, 690, 2322, 2649, 3552, 4836
BstACI GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
BstAFI CTTAAG 1 cut(s) 3839
BstAPI GCANNNNNTGC 3 cut(s) 2413, 3868, 4064
BstAUI TGTACA 1 cut(s) 918
BstBI TTCGAA 1 cut(s) 2916
BstDSI CCRYGG 1 cut(s) 1364
BstENI CCTNNNNNAGG 2 cut(s) 2436, 3405
BstFNI CGCG 2 cut(s) 556, 4411
BstHHI GCGC 5 cut(s) 487, 815, 2779, 3670, 5075
BstMCI CGRYCG 1 cut(s) 1131
BstNSI RCATGY 4 cut(s) 1964, 2482, 3217, 4076
BstSFI CTRYAG 7 cut(s) 1974, 3042, 3294, 3383, 4083, 4139, 4998
BstSLI GKGCMC 2 cut(s) 1090, 1762
BstUI CGCG 2 cut(s) 556, 4411
BstV2I GAAGAC 7 cut(s) 1368, 2286, 3311, 3555, 4245, 4647, 4986
BstX2I RGATCY 8 cut(s) 72, 172, 839, 1003, 1555, 1657, 1691, 4634
BstXI CCANNNNNNTGG 2 cut(s) 2125, 2951
BstYI RGATCY 8 cut(s) 72, 172, 839, 1003, 1555, 1657, 1691, 4634
Bsu36I CCTNAGG 2 cut(s) 1662, 4380
BsuRI GGCC 9 cut(s) 268, 646, 1110, 1159, 1252, 1680, 2117, 2173, 4183
BtgI CCRYGG 1 cut(s) 1364
BtgZI GCGATG 2 cut(s) 642, 4413
BtrI CACGTC 2 cut(s) 440, 776
BtsI GCAGTG 4 cut(s) 753, 1087, 3150, 3566
BveI ACCTGC 3 cut(s) 2812, 2818, 2943
CaiI CAGNNNCTG 6 cut(s) 1973, 2087, 2413, 2689, 3041, 4823
CciI TCATGA 2 cut(s) 1264, 5112
CfoI GCGC 5 cut(s) 487, 815, 2779, 3670, 5075
Cfr10I RCCGGY 7 cut(s) 399, 453, 642, 1986, 3806, 4165, 4237
CseI GACGC 5 cut(s) 1358, 2594, 2754, 4010, 4665
CsiI ACCWGGT 2 cut(s) 574, 1831
Csp6I GTAC 4 cut(s) 919, 1878, 3064, 4352
CspAI ACCGGT 4 cut(s) 399, 453, 1986, 3806
CviQI GTAC 4 cut(s) 919, 1878, 3064, 4352
DraIII CACNNNGTG 1 cut(s) 939
DriI GACNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 266, 2115
Eam1104I CTCTTC 8 cut(s) 16, 621, 667, 690, 2322, 2649, 3552, 4836
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 8 cut(s) 16, 621, 667, 690, 2322, 2649, 3552, 4836
EciI GGCGGA 2 cut(s) 13, 373
Ecl136II GAGCTC 1 cut(s) 3377
Eco130I CCWWGG 6 cut(s) 88, 1101, 1364, 2275, 2966, 3731
Eco147I AGGCCT 2 cut(s) 1159, 1252
Eco24I GRGCYC 2 cut(s) 3379, 4949
Eco31I GGTCTC 5 cut(s) 627, 646, 2334, 2733, 4857
Eco32I GATATC 2 cut(s) 85, 1699
Eco53kI GAGCTC 1 cut(s) 3377
Eco57I CTGAAG 8 cut(s) 266, 1275, 1410, 1653, 3291, 3555, 4714, 4823
Eco81I CCTNAGG 2 cut(s) 1662, 4380
Eco88I CYCGRG 1 cut(s) 4948
EcoICRI GAGCTC 1 cut(s) 3377
EcoNI CCTNNNNNAGG 2 cut(s) 2436, 3405
EcoO109I RGGNCCY 3 cut(s) 527, 1679, 4959
EcoRI GAATTC 1 cut(s) 3709
EcoRV GATATC 2 cut(s) 85, 1699
EcoT14I CCWWGG 6 cut(s) 88, 1101, 1364, 2275, 2966, 3731
EcoT22I ATGCAT 1 cut(s) 3946
EcoT38I GRGCYC 2 cut(s) 3379, 4949
ErhI CCWWGG 6 cut(s) 88, 1101, 1364, 2275, 2966, 3731
Esp3I CGTCTC 1 cut(s) 2772
FalI AAGNNNNNCTT 4 cut(s) 2612, 2644, 5051, 5083
FaqI GGGAC 4 cut(s) 338, 4245, 4307, 4972
FauI CCCGC 1 cut(s) 3327
FbaI TGATCA 2 cut(s) 2710, 2983
FriOI GRGCYC 2 cut(s) 3379, 4949
GlaI GCGC 5 cut(s) 486, 814, 2778, 3669, 5074
GsaI CCCAGC 2 cut(s) 526, 1722
GsuI CTGGAG 3 cut(s) 670, 891, 3215
HaeIII GGCC 9 cut(s) 268, 646, 1110, 1159, 1252, 1680, 2117, 2173, 4183
HgaI GACGC 5 cut(s) 1358, 2594, 2754, 4010, 4665
HhaI GCGC 5 cut(s) 487, 815, 2779, 3670, 5075
Hin1I GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
Hin6I GCGC 5 cut(s) 485, 813, 2777, 3668, 5073
HinP1I GCGC 5 cut(s) 485, 813, 2777, 3668, 5073
HincII GTYRAC 4 cut(s) 397, 2453, 2962, 3366
HindII GTYRAC 4 cut(s) 397, 2453, 2962, 3366
HindIII AAGCTT 3 cut(s) 1257, 1613, 3678
Hpy99I CGWCG 3 cut(s) 300, 441, 3986
HpyCH4IV ACGT 5 cut(s) 439, 775, 986, 1097, 3288
HpySE526I ACGT 5 cut(s) 439, 775, 986, 1097, 3288
Hsp92I GRCGYC 4 cut(s) 1097, 1369, 2765, 4676
HspAI GCGC 5 cut(s) 485, 813, 2777, 3668, 5073
KflI GGGWCCC 1 cut(s) 4959
Kpn2I TCCGGA 1 cut(s) 955
KroI GCCGGC 1 cut(s) 4165
KroNI GCCGGC 1 cut(s) 4167
Ksp22I TGATCA 2 cut(s) 2710, 2983
LguI GCTCTTC 2 cut(s) 690, 2649
MabI ACCWGGT 2 cut(s) 574, 1831
MaeII ACGT 5 cut(s) 439, 775, 986, 1097, 3288
MbiI CCGCTC 1 cut(s) 2137
MfeI CAATTG 2 cut(s) 2666, 5133
MflI RGATCY 8 cut(s) 72, 172, 839, 1003, 1555, 1657, 1691, 4634
MhlI GDGCHC 5 cut(s) 1024, 1090, 1762, 3379, 4949
MlsI TGGCCA 1 cut(s) 2117
MluNI TGGCCA 1 cut(s) 2117
MmeI TCCRAC 3 cut(s) 891, 2425, 5008
Mox20I TGGCCA 1 cut(s) 2117
Mph1103I ATGCAT 1 cut(s) 3946
MroI TCCGGA 1 cut(s) 955
MroNI GCCGGC 1 cut(s) 4165
MroXI GAANNNNTTC 9 cut(s) 193, 594, 684, 951, 3037, 3828, 4126, 4727, 4830
MscI TGGCCA 1 cut(s) 2117
MseI TTAA 7 cut(s) 2634, 3276, 3489, 3621, 3840, 4590, 5091
MslI CAYNNNNRTG 4 cut(s) 121, 198, 1494, 3861
Msp20I TGGCCA 1 cut(s) 2117
MspA1I CMGCKG 8 cut(s) 17, 354, 770, 1430, 1874, 2689, 3334, 5003
MspCI CTTAAG 1 cut(s) 3839
MunI CAATTG 2 cut(s) 2666, 5133
Mva1269I GAATGC 1 cut(s) 2578
MvnI CGCG 2 cut(s) 556, 4411
NaeI GCCGGC 1 cut(s) 4167
NcoI CCATGG 1 cut(s) 1364
NgoMIV GCCGGC 1 cut(s) 4165
NmuCI GTSAC 6 cut(s) 2805, 3899, 3955, 4666, 4677, 4856
NsiI ATGCAT 1 cut(s) 3946
NspI RCATGY 4 cut(s) 1964, 2482, 3217, 4076
NspV TTCGAA 1 cut(s) 2916
OliI CACNNNNGTG 1 cut(s) 121
PagI TCATGA 2 cut(s) 1264, 5112
PaqCI CACCTGC 2 cut(s) 2812, 2943
PceI AGGCCT 2 cut(s) 1159, 1252
PciI ACATGT 2 cut(s) 3213, 4072
PciSI GCTCTTC 2 cut(s) 690, 2649
PcsI WCGNNNNNNNCGW 1 cut(s) 301
PctI GAATGC 1 cut(s) 2578
PdiI GCCGGC 1 cut(s) 4167
PdmI GAANNNNTTC 9 cut(s) 193, 594, 684, 951, 3037, 3828, 4126, 4727, 4830
PflFI GACNNNGTC 1 cut(s) 441
PflMI CCANNNNNTGG 2 cut(s) 1832, 2750
PfoI TCCNGGA 3 cut(s) 868, 3323, 4579
PinAI ACCGGT 4 cut(s) 399, 453, 1986, 3806
PpuMI RGGWCCY 2 cut(s) 527, 4959
PscI ACATGT 2 cut(s) 3213, 4072
PsiI TTATAA 1 cut(s) 1653
Psp124BI GAGCTC 1 cut(s) 3379
Psp5II RGGWCCY 2 cut(s) 527, 4959
PspFI CCCAGC 2 cut(s) 522, 1718
PspPPI RGGWCCY 2 cut(s) 527, 4959
PsrI GAACNNNNNNTAC 2 cut(s) 3047, 3079
PstI CTGCAG 3 cut(s) 3046, 3387, 4143
PstNI CAGNNNCTG 6 cut(s) 1973, 2087, 2413, 2689, 3041, 4823
PsuI RGATCY 8 cut(s) 72, 172, 839, 1003, 1555, 1657, 1691, 4634
PsyI GACNNNGTC 1 cut(s) 441
PvuII CAGCTG 5 cut(s) 770, 1430, 1874, 2689, 5003
RsaI GTAC 4 cut(s) 920, 1879, 3065, 4353
RsaNI GTAC 4 cut(s) 919, 1878, 3064, 4352
RseI CAYNNNNRTG 4 cut(s) 121, 198, 1494, 3861
SacI GAGCTC 1 cut(s) 3379
SapI GCTCTTC 2 cut(s) 690, 2649
SaqAI TTAA 7 cut(s) 2634, 3276, 3489, 3621, 3840, 4590, 5091
SduI GDGCHC 5 cut(s) 1024, 1090, 1762, 3379, 4949
SexAI ACCWGGT 2 cut(s) 574, 1831
SfcI CTRYAG 7 cut(s) 1974, 3042, 3294, 3383, 4083, 4139, 4998
SfuI TTCGAA 1 cut(s) 2916
SmiMI CAYNNNNRTG 4 cut(s) 121, 198, 1494, 3861
SmlI CTYRAG 6 cut(s) 660, 2031, 2132, 2517, 3839, 5068
SmoI CTYRAG 6 cut(s) 660, 2031, 2132, 2517, 3839, 5068
SseBI AGGCCT 2 cut(s) 1159, 1252
SstI GAGCTC 1 cut(s) 3379
StuI AGGCCT 2 cut(s) 1159, 1252
StyI CCWWGG 6 cut(s) 88, 1101, 1364, 2275, 2966, 3731
TaiI ACGT 5 cut(s) 442, 778, 989, 1100, 3291
TatI WGTACW 2 cut(s) 918, 3063
TauI GCSGC 4 cut(s) 29, 1519, 2140, 3728
Tru1I TTAA 7 cut(s) 2634, 3276, 3489, 3621, 3840, 4590, 5091
Tru9I TTAA 7 cut(s) 2634, 3276, 3489, 3621, 3840, 4590, 5091
TseFI GTSAC 6 cut(s) 2805, 3899, 3955, 4666, 4677, 4856
Tsp45I GTSAC 6 cut(s) 2805, 3899, 3955, 4666, 4677, 4856
TspGWI ACGGA 6 cut(s) 43, 1016, 1165, 2148, 2300, 3316
Tth111I GACNNNGTC 1 cut(s) 441
Van91I CCANNNNNTGG 2 cut(s) 1832, 2750
Vha464I CTTAAG 1 cut(s) 3839
VneI GTGCAC 1 cut(s) 1758
XagI CCTNNNNNAGG 2 cut(s) 2436, 3405
XapI RAATTY 8 cut(s) 422, 1859, 2385, 2425, 3642, 3709, 4793, 4883
XbaI TCTAGA 1 cut(s) 3371
XceI RCATGY 4 cut(s) 1964, 2482, 3217, 4076
XcmI CCANNNNNNNNNTGG 1 cut(s) 3597
XmaJI CCTAGG 1 cut(s) 2966
XmnI GAANNNNTTC 9 cut(s) 193, 594, 684, 951, 3037, 3828, 4126, 4727, 4830
ZraI GACGTC 1 cut(s) 1098
Zsp2I ATGCAT 1 cut(s) 3946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.