MD08G1034300.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008668
Physical Location & Seq
Reverse (-)
2410041 .. 2417811
7771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1034300.v1.1.491

Sequence Viewer

Length: 5400 bp
ATGGCCGACCTCTCCAGCTCAGCCTCTCGCCGCCATCTCTCCGTCACCACTCCGCCCCAGATCTCCAAAGGTGGACAAGGTTCTGAGAATCTTATTCCCCTTTCACCGCAGTGGCTTCTACCTAAGCCGGGGGAGAGTAAGACAGGAATGTTAACTGGGGAAAAACCTCCCAGCCCAAATCCATCCTTTGGCAGTCGCTTGGATACCACGAAATCATCAGGTAATGGTGATGAAATTCGTGATACCCAGAAGAAGGATGTTTTCAGGCCATCTTTGATGGATATGGAAGCTGGTGGTCGTCGTGAATGCTGGCGTGATGAAGAAAGAGACACCAACTCCTCAGGACGCAAAGATCGTTGGAGGGATGGGGATAAAGAAGTTGGTGACACTCGCAGGATGGATCGATGGATGGAAAATTCATCTGCAAAGCACTTTGGAGAAGCACGTCGTGCCCCACCTGAGCGGTGGACTGACTCAAGTAACAAGGAATCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACACACGCTGGGGGCCTAATGACAAGGAGGCAGAAGGTTTTCCTGACAAGTGGGCAGACTCTGGTAGAGATGGTCTGCATCTTGATAAGAGTTTGTCTCATGTTGGAAATCATGTAAAGGATGAGAAAGATGGGGATCACTACCGACCTTGGAGATCAAACTCCTCCCAAGCCCGGGGAAGGGGAGATCCTTCTTATAACCAGACTTTATCAGCAAACAAGCATGCTCCTACACATTCATCTAATTGGGGGCGTGGAGAAATTACACCTCCCAGCTTTTCTCTTGGTCGTGGAAGGGGTAGCTCTGGTGGAGGCTTTATGAACAGCAGTCCTACTATTCCTCAGTCTATAGGAACTGTATTGGACAAAGTTGAAAGTGAACATGGAGAGCCTTATCCATTAAGGTATAGTAGGACGAAGCTGCTTGATGTATACAGGAAGGCTGATATGAAATCATATCAAAAACCAGTTGATGGGTTTATAGAAGTGAGCTCTCTTGCGGTGGATGAACCATTGGAGCCTCTGGCACTTTGTGCACCGAATTCTGAGGAAATGTCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCCCAGACTTCTAAAGATGGAAGGAACCCAAATGATTTTACACAGTCAAGAAGACCCAAGCTTGGCAGTAGAGAAGATTTACCCCTTGCACTTGGTGATTCTAAAGATGAAAGCATTGCTAGATCAAAAGGTGGTCTTCCAAATTATTCAGAGGGCTCTTCTTATGAAAGGCAGGTGCTCCATCATGGATCCAGCCTAAAAGGAGAAATAACGCAGGAACATAAGACATACTCAGAGAACAATTTCAGATCGGAAGCTTTTAGAGGAGACAGTGGTCCTTTCATAAGGGCTGAGGAGGCACCTGTTAGTGCAGATTTGAGCATGCAGGGAGGTATTACTGCTCATTCTGGTTCTCCATGGAGATCTTCATCACAAGGAGAACGCTCACATACAGTCTTGCATGATTGGAACGAGATTCCCGGTGATATTAAGTCAAGAACTCCTGATATGGGCTGGTCGCTAGGATCGAAAGATCTTAATAACGAATGGGAAAGTAGGGATGAGGCAAGATGGAAAACCAGTGATGATCCTGTTATAAGGAGGCAGCGATCGGGAATTCTAGATAGGGAACAAGAATTGAGACAACCTCAGCAGCTTTCTCCAGAGGAGCTTCAGCTTTATTATAAAGATCCTCAGGGTATAATTCAAGGTCCTTTCTCTGGGGCTGACATTATTGGCTGGTTTGAGGCTGGATATTTTGGCATAGATTTGCTAGTTCGCATAGCAGGTGCAAAAAATGATACGCCATTTTTGGCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGACCACCGCCTGGATTTACTGCACCTAAACCAGAGGTTGCAGATACATCAGGTAGGCCAAACTTTGGTAATGTTGGGATGATTCAAGCTGGTTTAAGTGAGATAGAGATAGCAAGAAATGATCCAAGGAATAAACAGGGCTCGACAACAGAAGCTCAGAATAGGTTTCTGGAGTCACTGATGTCTGGTAATCCGAGTGGCTTACCACTTCATAAGTTTCCTTTTTCGGAAGGTTTACAAGGATTTATGGGAAACAATCCTCACGGCTTGACTCATAGTGGATTGGATAATCTGTTGGCCAAGAGAATGGTTCTCGAACGACAGAGGTCTTTTCCTAATCCTTATCAATATTGGCCTGGGAGGGATGCTTCATCTGTGATGCCTAAGTCGGAGATTGTCCCAGATGCAAACCTCCTCTCTTCAGTGGCTGAAAATCCTAGTCAGCCTCCTCATACCCAAAATGCTGACCTCATGTCCATCCTTCAAGGATTAACTAACCGGTCATCATCTGACATAAATAATAGTACTGCTGGTCGGTCAACTTTTCCTGTCCAAGGTGGCTCAGAGCCACTCCAGAGTAATTTTCCCCCCCAAGCCCCACTCCAGAGTAACTTTCCTCCCCAAATCCCACTTGGGTTCCAACAGCAGAGGCTGCATCCTCAGAATCAACAATCTTTACTCTCTCAAGCTATTGATAGTTCAAGTATTTCAACGCAAGAGAAATTACTATCTTCTGGTTTATTACAAGATCCACAAGTATTGAGTATGTTGCAACAGCAGTATTTATTGCAGTTACATTCCCAGGCGCCTGTTCCAACACAACAAATGTCATTGTTGGATAATTTAATGTTGCTCAAGCAGCAGCAACAGAAACAGGAGGAGCAGCAAATTTTGTTAAGGCAACAACAGCAGTTGCTTTCCCAGGTTATGCCAGAGCATCACTCTCAACAGCATTTTACTGAGCCATCTTTTGGACAGATGCAGGCTTCTGCGATGCTGAAAGGGATAGATCCCTCCCGGCTTCAGTCGTCACAAGAAATGTTTCCAGTTGGTACAAATGTGCAGGTTGCTAATACGCAAAATGAACTTACTAGTAGTTATATGACTTTGCCTCCCCAAGGTAACCTAGATATCAGTCATAATGTTAGTGAGGGAGCATCCTCAGTACCTTTACCGCATCAAGTGCTTGGGAATATTACCCATCAAAGAAGTCGGGATGTTACTCCGGCGGCACCAATTTCTATTCAGCAGGAGTCGCTGCCAATGTCCACAAATGTTGAGAGCTCACCGTTGTTTGATGTGATGACCAAATCTAGAGAGGTGTCCCTTGCGCAGGAGTCCATACCTGATTCTGATTTTCATGTTGCTAAAACCCTGGAAGAGGCAGCTGAAAACGCTTTCATAGCTGACCGATCTGCTGGGGTAGCAATTTCTCAGGGTGTTGCAGATCCTCTGCGATCATTAGGAGCTTTTGAGAAAGATGTACCTGAGCATATCAATGATGTGAATGTTCAATCAGGCAATCAAATTGAAGAACAGCAGATTGAAAGGGAAAAATACAATGATGAGGCTCCTACAGTGGTTGAAGCAAAGAATGTTGAAGTAAGAGGGCAAAGAAAGATTTCTGAGAAGAAGTCCAAGAAGCAAAAATCTGCCAAGGCACAGTCTTCAAGTGACCAAGCAAAAGGAGCATCCAAGGCGCAGTCTGAAGCTGAAAAGCCTGTTGTTGGTGACGTAAAATTGGAGACGAGAGGCAACCGAGGCAGTAAGCCTGAAATTGTTACAGTGGAGGCTGTGGAATCCCGACAATCTGAAAATTTAGAGCCTGTGAAAGTTTCTGGAGATGATACAGAACCTCATGAAATCAGGGGTGATTCTAAGTTAGTTGAGTCTGTTTCTGGGCAAAGTACTCATACACAGATTGGCCAGCGAGCTTGGAAGCCTGCTCCTGGTTTTAAGGCAAAATCGTTACTAGAAATTCAACAGGAAGAACAGAGGAAGGCAGAGTCAGAAGTGATTATACCTGAGGTCACCATCAACTCCCCCAGTTTGTCAACTCCTTGGGCCGGGGTTGTAGCCAATTCAGAACCAAAAATATCTAGAGAAACTCCGGTAGATGCAGGTATTAATGACTTAAATGCTGGGAAGCCCAAAACTTCTCAAAACTCAAAGAGCAAGAAGAGCCCGCTACATGATTTATTAGCAGAGGAGGTTTTGGCCAAGTCCAGTGAAAGAGATGTTGAAATTCCTAATGGTGTGTCAACTCAGCATTCTTCACAAATTATGCCCACCCATTCAGAATCCGTAGATGACGATAACTTTATTGAGGCAAAAGACACCAAAAAGAGTCGCAAAAGGTCAGCAAAAGCGAAGGGTACTGGAACCAAGACCTCAATATCAGTTACTGCTGTAGATGTGCCTGTTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAGCAGGAAAAGGAAGTATTGCCTGCAATTCCATCAGGGCCTTCATTGGGAGATTTTGTTCCTTGGAAAGGAGAAACGGCTAACCCTGCTCCTTCTCCAGCATGGCTTACAGACTCCGGGAAGATTCCTAAACCCACATCTTTAAGAGATATCCAAAAGGAGCAGGAGAAGAGGGTTGCTGCTGCTCAGCACCAAAATCAGATTCCAACTCCAAAGAAATCACAGCCACCTCAAGTCACCCGCAATAGTGCTCCGTCATGGTCGCTTTCAGCATCATCTCCATCTAAAGCTGCATCTCCCATCGCAATTAATTCCCATGCATCTCAGTCAAGAAATAAAGTAGAAGATGACTTATTCTGGGGACCAATTGATCAAGCAAAGCAAGAAAATAAGCAGGCAGATTTTCCTCATCTTGCAAGCCAGGGCAGTCAGGGAGTGAAAAATACTCCTGTAAAAGGAACTTCAGCTGGTTCATTAGGCAGGCAGAAATCAGTGGGTGGAAAGTCAAATGAGCGATTACTTTCATCCTCACCGTCTCAGTCATCTACAAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCCATGGGCTTCCAAGATTGGTGCAAGAGCGAGTGCGTTAGGCTTATTGGCACGAAAGATACGAGTTTCCTTGAGTTTTGCCTGAAGCAATCCAAATCGGAGGCGGAGTTGCTTCTGAAACAGAACCTAGGGTCGTATGATCCCGATCATGAGTTCATTGACAAATTTCTCAACTACAAGGAGTTGCTACCGGCTGATGTCCTTGAAATTGCCTTTCAAAAAGGGAATGACCAAAGAGGGGTCGCTGGGTTCAGCGGCGGAGATGTGAATTCATACAGTGCAGATGTTGGGGATGTGGACCAAGACGGGTTCTCCAAAGGGGGCGGCAAGAAGAAGGGGAAGAAAGGAAAGAAGGTTAGTCCGGCTGTCTTGGGATTTAACGTCGTGAGCAACCGCATCATGATGGGTGAGATCCAGACACTGGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1800

Amino Acids

196.97

Weight (kDa)

6.25

Isoelectric Point (pI)

58.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 582 - 619 2.7e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 720, 1661, 1749
AarI CACCTGC 2 cut(s) 1288, 1841
Acc16I TGCGCA 1 cut(s) 3249
Acc36I ACCTGC 4 cut(s) 1288, 1841, 2971, 4028
AccB1I GGYRCC 3 cut(s) 1423, 2722, 3148
AccB7I CCANNNNNTGG 6 cut(s) 188, 995, 1928, 1982, 2888, 5389
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 954
AcoI YGGCCR 4 cut(s) 3, 2214, 3841, 4134
AcuI CTGAAG 6 cut(s) 1721, 2322, 2924, 3645, 4830, 5072
AcyI GRCGYC 1 cut(s) 2723
AdeI CACNNNGTG 4 cut(s) 449, 1055, 1220, 1886
AfaI GTAC 7 cut(s) 2443, 2971, 3084, 3402, 3826, 4294, 4383
AgeI ACCGGT 1 cut(s) 2415
AhdI GACNNNNNGTC 1 cut(s) 2389
AhlI ACTAGT 1 cut(s) 3008
AjiI CACGTC 1 cut(s) 446
AjnI CCWGG 7 cut(s) 1927, 2272, 2718, 2838, 3291, 3865, 4803
AjuI GAANNNNNNNTTGG 2 cut(s) 4569, 4601
AleI CACNNNNGTG 1 cut(s) 109
AloI GAACNNNNNNTCC 2 cut(s) 4425, 4457
Alw21I GWGCWC 6 cut(s) 1016, 1060, 1126, 1305, 3203, 4636
Alw26I GTCTC 7 cut(s) 321, 624, 1083, 1386, 1699, 3656, 4923
Alw44I GTGCAC 2 cut(s) 1056, 1122
AlwNI CAGNNNCTG 5 cut(s) 584, 2345, 2569, 4322, 5389
Ama87I CYCGRG 1 cut(s) 696
ApaLI GTGCAC 2 cut(s) 1056, 1122
AseI ATTAAT 2 cut(s) 4044, 4692
AsiGI ACCGGT 1 cut(s) 2415
Asp700I GAANNNNTTC 4 cut(s) 561, 1367, 2958, 3538
AspA2I CCTAGG 1 cut(s) 5095
AspLEI GCGC 3 cut(s) 2725, 3250, 3619
AspS9I GGNCC 7 cut(s) 536, 1400, 1775, 3981, 4421, 4745, 5266
AsuC2I CCSGG 7 cut(s) 129, 697, 698, 1545, 2935, 3985, 4501
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 4 cut(s) 1400, 1775, 4745, 5266
AvrII CCTAGG 1 cut(s) 5095
AxyI CCTNAGG 3 cut(s) 340, 1758, 3942
BaeGI GKGCMC 3 cut(s) 454, 1060, 1126
BaeI ACNNNNGTAYC 2 cut(s) 2961, 2994
BalI TGGCCA 3 cut(s) 2216, 3843, 4136
BamHI GGATCC 1 cut(s) 1313
BanI GGYRCC 3 cut(s) 1423, 2722, 3148
BanII GRGCYC 5 cut(s) 1016, 1283, 2060, 3203, 4103
BarI GAAGNNNNNNTAC 2 cut(s) 3921, 3953
BbsI GAAGAC 3 cut(s) 1183, 1253, 3576
Bbv12I GWGCWC 6 cut(s) 1016, 1060, 1126, 1305, 3203, 4636
BbvCI CCTCAGC 2 cut(s) 1416, 1713
BceAI ACGGC 3 cut(s) 521, 2197, 4476
BciT130I CCWGG 7 cut(s) 1929, 2274, 2720, 2840, 3293, 3867, 4805
BciVI GTATCC 1 cut(s) 196
BclI TGATCA 1 cut(s) 4753
BcnI CCSGG 7 cut(s) 129, 697, 698, 1545, 2935, 3985, 4501
BcoDI GTCTC 7 cut(s) 321, 624, 1083, 1386, 1699, 3656, 4923
BcuI ACTAGT 1 cut(s) 3008
BfmI CTRYAG 3 cut(s) 870, 3492, 4326
BfoI RGCGCY 1 cut(s) 2726
BfuAI ACCTGC 4 cut(s) 1288, 1841, 2971, 4028
BfuI GTATCC 1 cut(s) 196
BglII AGATCT 3 cut(s) 60, 1487, 1597
BlnI CCTAGG 1 cut(s) 5095
BlpI GCTNAGC 2 cut(s) 19, 4569
BmcAI AGTACT 2 cut(s) 2443, 3826
Bme18I GGWCC 4 cut(s) 1400, 1775, 4745, 5266
BmeRI GACNNNNNGTC 1 cut(s) 2389
BmeT110I CYCGRG 1 cut(s) 696
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 7 cut(s) 536, 1400, 1775, 3981, 4421, 4745, 5266
BmrI ACTGGG 2 cut(s) 165, 3957
BmuI ACTGGG 2 cut(s) 165, 3957
BoxI GACNNNNGTC 2 cut(s) 1398, 2242
BpiI GAAGAC 3 cut(s) 1183, 1253, 3576
BplI GAGNNNNNCTC 6 cut(s) 604, 636, 1696, 1728, 2843, 2875
BpmI CTGGAG 6 cut(s) 1710, 2108, 2474, 2504, 3777, 4464
Bpu10I CCTNAGC 5 cut(s) 123, 459, 1416, 1713, 3405
Bpu1102I GCTNAGC 2 cut(s) 19, 4569
BpuEI CTTGAG 5 cut(s) 460, 2586, 2756, 4599, 5060
BpuMI CCSGG 7 cut(s) 129, 697, 698, 1545, 2935, 3985, 4501
Bsa29I ATCGAT 1 cut(s) 403
BsaBI GATNNNNATC 3 cut(s) 657, 1492, 5112
BsaHI GRCGYC 1 cut(s) 2723
BsaWI WCCGGW 2 cut(s) 2415, 4027
Bse118I RCCGGY 2 cut(s) 2415, 5158
Bse1I ACTGG 8 cut(s) 160, 989, 1644, 2963, 3963, 4143, 4300, 5394
Bse21I CCTNAGG 3 cut(s) 340, 1758, 3942
Bse3DI GCAATG 1 cut(s) 1239
Bse8I GATNNNNATC 3 cut(s) 657, 1492, 5112
BseBI CCWGG 7 cut(s) 1929, 2274, 2720, 2840, 3293, 3867, 4805
BseCI ATCGAT 1 cut(s) 403
BseJI GATNNNNATC 3 cut(s) 657, 1492, 5112
BseMI GCAATG 1 cut(s) 1239
BseNI ACTGG 8 cut(s) 160, 989, 1644, 2963, 3963, 4143, 4300, 5394
BseRI GAGGAG 9 cut(s) 328, 676, 1404, 1433, 1745, 2321, 2355, 2810, 4139
BseSI GKGCMC 3 cut(s) 454, 1060, 1126
BseYI CCCAGC 6 cut(s) 170, 531, 794, 3335, 4058, 5213
BsgI GTGCAG 4 cut(s) 1455, 1923, 2999, 5268
Bsh1285I CGRYCG 1 cut(s) 1676
BshNI GGYRCC 3 cut(s) 1423, 2722, 3148
BshTI ACCGGT 1 cut(s) 2415
BshVI ATCGAT 1 cut(s) 403
BsiEI CGRYCG 1 cut(s) 1676
BsiHKAI GWGCWC 6 cut(s) 1016, 1060, 1126, 1305, 3203, 4636
BsiHKCI CYCGRG 1 cut(s) 696
BslFI GGGAC 3 cut(s) 2300, 3226, 4758
BsmAI GTCTC 7 cut(s) 321, 624, 1083, 1386, 1699, 3656, 4923
BsmBI CGTCTC 2 cut(s) 3656, 4923
BsmFI GGGAC 3 cut(s) 2300, 3226, 4758
BsmI GAATGC 2 cut(s) 311, 4186
BsoBI CYCGRG 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 4381
Bsp1720I GCTNAGC 2 cut(s) 19, 4569
Bsp19I CCATGG 2 cut(s) 1481, 4971
BspDI ATCGAT 1 cut(s) 403
BspHI TCATGA 3 cut(s) 3775, 5116, 5367
BspMI ACCTGC 4 cut(s) 1288, 1841, 2971, 4028
BspQI GCTCTTC 2 cut(s) 1288, 4091
BspT107I GGYRCC 3 cut(s) 1423, 2722, 3148
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 1239
BsrFI RCCGGY 2 cut(s) 2415, 5158
BsrGI TGTACA 1 cut(s) 4381
BsrI ACTGG 8 cut(s) 160, 989, 1644, 2963, 3963, 4143, 4300, 5394
BssAI RCCGGY 2 cut(s) 2415, 5158
BssNAI GTATAC 1 cut(s) 955
BssNI GRCGYC 1 cut(s) 2723
Bst1107I GTATAC 1 cut(s) 955
Bst2UI CCWGG 7 cut(s) 1929, 2274, 2720, 2840, 3293, 3867, 4805
Bst6I CTCTTC 5 cut(s) 1288, 2341, 3291, 4091, 4547
BstACI GRCGYC 1 cut(s) 2723
BstAPI GCANNNNNTGC 4 cut(s) 449, 1055, 2569, 3100
BstAUI TGTACA 1 cut(s) 4381
BstDSI CCRYGG 2 cut(s) 1481, 4971
BstEII GGTNACC 2 cut(s) 3038, 3946
BstENI CCTNNNNNAGG 6 cut(s) 2469, 3033, 3248, 3296, 4449, 4836
BstH2I RGCGCY 1 cut(s) 2726
BstHHI GCGC 3 cut(s) 2725, 3250, 3619
BstMAI GTCTC 7 cut(s) 321, 624, 1083, 1386, 1699, 3656, 4923
BstMCI CGRYCG 1 cut(s) 1676
BstNI CCWGG 7 cut(s) 1929, 2274, 2720, 2840, 3293, 3867, 4805
BstNSI RCATGY 2 cut(s) 749, 1450
BstPAI GACNNNNGTC 2 cut(s) 1398, 2242
BstPI GGTNACC 2 cut(s) 3038, 3946
BstSFI CTRYAG 3 cut(s) 870, 3492, 4326
BstSLI GKGCMC 3 cut(s) 454, 1060, 1126
BstV2I GAAGAC 3 cut(s) 1183, 1253, 3576
BstXI CCANNNNNNTGG 1 cut(s) 2224
BstZ17I GTATAC 1 cut(s) 955
Bsu15I ATCGAT 1 cut(s) 403
Bsu36I CCTNAGG 3 cut(s) 340, 1758, 3942
BsuI GTATCC 1 cut(s) 196
BsuTUI ATCGAT 1 cut(s) 403
BtgI CCRYGG 2 cut(s) 1481, 4971
BtgZI GCGATG 2 cut(s) 2924, 4669
BtrI CACGTC 1 cut(s) 446
BtsI GCAGTG 1 cut(s) 116
BveI ACCTGC 4 cut(s) 1288, 1841, 2971, 4028
CaiI CAGNNNCTG 5 cut(s) 584, 2345, 2569, 4322, 5389
CciI TCATGA 3 cut(s) 3775, 5116, 5367
CfoI GCGC 3 cut(s) 2725, 3250, 3619
Cfr10I RCCGGY 2 cut(s) 2415, 5158
Cfr13I GGNCC 7 cut(s) 536, 1400, 1775, 3981, 4421, 4745, 5266
Cfr9I CCCGGG 1 cut(s) 696
ClaI ATCGAT 1 cut(s) 403
CseI GACGC 1 cut(s) 354
Csp6I GTAC 7 cut(s) 2442, 2970, 3083, 3401, 3825, 4293, 4382
CspAI ACCGGT 1 cut(s) 2415
CspCI CAANNNNNGTGG 4 cut(s) 2658, 2693, 3175, 3210
CviQI GTAC 7 cut(s) 2442, 2970, 3083, 3401, 3825, 4293, 4382
DinI GGCGCC 1 cut(s) 2724
DraIII CACNNNGTG 4 cut(s) 449, 1055, 1220, 1886
DriI GACNNNNNGTC 1 cut(s) 2389
EaeI YGGCCR 4 cut(s) 3, 2214, 3841, 4134
Eam1104I CTCTTC 5 cut(s) 1288, 2341, 3291, 4091, 4547
Eam1105I GACNNNNNGTC 1 cut(s) 2389
EarI CTCTTC 5 cut(s) 1288, 2341, 3291, 4091, 4547
EciI GGCGGA 3 cut(s) 42, 5087, 5241
Ecl136II GAGCTC 2 cut(s) 1014, 3201
Eco24I GRGCYC 5 cut(s) 1016, 1283, 2060, 3203, 4103
Eco32I GATATC 2 cut(s) 3049, 4534
Eco47I GGWCC 4 cut(s) 1400, 1775, 4745, 5266
Eco53kI GAGCTC 2 cut(s) 1014, 3201
Eco57I CTGAAG 6 cut(s) 1721, 2322, 2924, 3645, 4830, 5072
Eco81I CCTNAGG 3 cut(s) 340, 1758, 3942
Eco88I CYCGRG 1 cut(s) 696
Eco91I GGTNACC 2 cut(s) 3038, 3946
EcoICRI GAGCTC 2 cut(s) 1014, 3201
EcoNI CCTNNNNNAGG 6 cut(s) 2469, 3033, 3248, 3296, 4449, 4836
EcoO109I RGGNCCY 3 cut(s) 536, 1775, 4421
EcoO65I GGTNACC 2 cut(s) 3038, 3946
EcoRI GAATTC 3 cut(s) 1063, 1680, 5236
EcoRII CCWGG 7 cut(s) 1927, 2272, 2718, 2838, 3291, 3865, 4803
EcoRV GATATC 2 cut(s) 3049, 4534
EcoT22I ATGCAT 1 cut(s) 4705
EcoT38I GRGCYC 5 cut(s) 1016, 1283, 2060, 3203, 4103
EgeI GGCGCC 1 cut(s) 2724
EheI GGCGCC 1 cut(s) 2724
Esp3I CGTCTC 2 cut(s) 3656, 4923
FalI AAGNNNNNCTT 2 cut(s) 1245, 1277
FaqI GGGAC 3 cut(s) 2300, 3226, 4758
FauI CCCGC 2 cut(s) 4110, 4631
FbaI TGATCA 1 cut(s) 4753
FblI GTMKAC 1 cut(s) 954
FriOI GRGCYC 5 cut(s) 1016, 1283, 2060, 3203, 4103
FspI TGCGCA 1 cut(s) 3249
GlaI GCGC 3 cut(s) 2724, 3249, 3618
GsaI CCCAGC 6 cut(s) 174, 535, 798, 3339, 4062, 5217
GsuI CTGGAG 6 cut(s) 1710, 2108, 2474, 2504, 3777, 4464
HaeII RGCGCY 1 cut(s) 2726
HgaI GACGC 1 cut(s) 354
HhaI GCGC 3 cut(s) 2725, 3250, 3619
Hin1I GRCGYC 1 cut(s) 2723
Hin6I GCGC 3 cut(s) 2723, 3248, 3617
HinP1I GCGC 3 cut(s) 2723, 3248, 3617
HincII GTYRAC 4 cut(s) 153, 2457, 3972, 4179
HindII GTYRAC 4 cut(s) 153, 2457, 3972, 4179
HindIII AAGCTT 2 cut(s) 1184, 1380
HpaI GTTAAC 1 cut(s) 153
Hpy99I CGWCG 3 cut(s) 303, 450, 5354
HpyCH4IV ACGT 3 cut(s) 445, 3651, 5349
HpySE526I ACGT 3 cut(s) 445, 3651, 5349
Hsp92I GRCGYC 1 cut(s) 2723
HspAI GCGC 3 cut(s) 2723, 3248, 3617
KasI GGCGCC 1 cut(s) 2722
Ksp22I TGATCA 1 cut(s) 4753
KspAI GTTAAC 1 cut(s) 153
LguI GCTCTTC 2 cut(s) 1288, 4091
MaeII ACGT 3 cut(s) 445, 3651, 5349
MbiI CCGCTC 1 cut(s) 463
MfeI CAATTG 1 cut(s) 4749
MlsI TGGCCA 3 cut(s) 2216, 3843, 4136
MluNI TGGCCA 3 cut(s) 2216, 3843, 4136
Mly113I GGCGCC 1 cut(s) 2723
MmeI TCCRAC 7 cut(s) 338, 607, 2286, 2581, 2733, 2756, 4613
Mox20I TGGCCA 3 cut(s) 2216, 3843, 4136
Mph1103I ATGCAT 1 cut(s) 4705
MroXI GAANNNNTTC 4 cut(s) 561, 1367, 2958, 3538
MscI TGGCCA 3 cut(s) 2216, 3843, 4136
MslI CAYNNNNRTG 3 cut(s) 109, 3414, 5369
Msp20I TGGCCA 3 cut(s) 2216, 3843, 4136
MspA1I CMGCKG 3 cut(s) 3305, 4850, 5223
MunI CAATTG 1 cut(s) 4749
Mva1269I GAATGC 2 cut(s) 311, 4186
MvaI CCWGG 7 cut(s) 1929, 2274, 2720, 2840, 3293, 3867, 4805
NarI GGCGCC 1 cut(s) 2723
NciI CCSGG 7 cut(s) 129, 697, 698, 1545, 2935, 3985, 4501
NcoI CCATGG 2 cut(s) 1481, 4971
NmuCI GTSAC 8 cut(s) 43, 383, 2091, 2946, 3590, 3647, 3946, 4618
NsbI TGCGCA 1 cut(s) 3249
NsiI ATGCAT 1 cut(s) 4705
NspI RCATGY 2 cut(s) 749, 1450
OliI CACNNNNGTG 1 cut(s) 109
PaeI GCATGC 2 cut(s) 749, 1450
PagI TCATGA 3 cut(s) 3775, 5116, 5367
PaqCI CACCTGC 2 cut(s) 1288, 1841
PciSI GCTCTTC 2 cut(s) 1288, 4091
PcsI WCGNNNNNNNCGW 3 cut(s) 352, 1589, 5027
PctI GAATGC 2 cut(s) 311, 4186
PdmI GAANNNNTTC 4 cut(s) 561, 1367, 2958, 3538
PflMI CCANNNNNTGG 6 cut(s) 188, 995, 1928, 1982, 2888, 5389
PfoI TCCNGGA 1 cut(s) 4499
PinAI ACCGGT 1 cut(s) 2415
Ple19I CGATCG 1 cut(s) 1676
PluTI GGCGCC 1 cut(s) 2726
PpuMI RGGWCCY 1 cut(s) 1775
PshAI GACNNNNGTC 2 cut(s) 1398, 2242
PshBI ATTAAT 2 cut(s) 4044, 4692
PsiI TTATAA 3 cut(s) 720, 1661, 1749
Psp124BI GAGCTC 2 cut(s) 1016, 3203
Psp5II RGGWCCY 1 cut(s) 1775
Psp6I CCWGG 7 cut(s) 1927, 2272, 2718, 2838, 3291, 3865, 4803
PspEI GGTNACC 2 cut(s) 3038, 3946
PspFI CCCAGC 6 cut(s) 170, 531, 794, 3335, 4058, 5213
PspGI CCWGG 7 cut(s) 1927, 2272, 2718, 2838, 3291, 3865, 4803
PspPI GGNCC 7 cut(s) 536, 1400, 1775, 3981, 4421, 4745, 5266
PspPPI RGGWCCY 1 cut(s) 1775
PstNI CAGNNNCTG 5 cut(s) 584, 2345, 2569, 4322, 5389
PvuI CGATCG 1 cut(s) 1676
PvuII CAGCTG 2 cut(s) 3305, 4850
RsaI GTAC 7 cut(s) 2443, 2971, 3084, 3402, 3826, 4294, 4383
RsaNI GTAC 7 cut(s) 2442, 2970, 3083, 3401, 3825, 4293, 4382
RseI CAYNNNNRTG 3 cut(s) 109, 3414, 5369
SacI GAGCTC 2 cut(s) 1016, 3203
SapI GCTCTTC 2 cut(s) 1288, 4091
Sau96I GGNCC 7 cut(s) 536, 1400, 1775, 3981, 4421, 4745, 5266
ScaI AGTACT 2 cut(s) 2443, 3826
SfcI CTRYAG 3 cut(s) 870, 3492, 4326
SfoI GGCGCC 1 cut(s) 2724
SinI GGWCC 4 cut(s) 1400, 1775, 4745, 5266
SmaI CCCGGG 1 cut(s) 698
SmiMI CAYNNNNRTG 3 cut(s) 109, 3414, 5369
SmlI CTYRAG 5 cut(s) 475, 2601, 2771, 4614, 5039
SmoI CTYRAG 5 cut(s) 475, 2601, 2771, 4614, 5039
SpeI ACTAGT 1 cut(s) 3008
SphI GCATGC 2 cut(s) 749, 1450
SspDI GGCGCC 1 cut(s) 2722
SspI AATATT 2 cut(s) 2267, 3112
SstI GAGCTC 2 cut(s) 1016, 3203
TaiI ACGT 3 cut(s) 448, 3654, 5352
TaqI TCGA 4 cut(s) 403, 1592, 2060, 2232
TaqII GACCGA 2 cut(s) 2442, 3342
TatI WGTACW 3 cut(s) 2441, 3824, 4381
TauI GCSGC 4 cut(s) 33, 3149, 5226, 5295
TseFI GTSAC 8 cut(s) 43, 383, 2091, 2946, 3590, 3647, 3946, 4618
Tsp45I GTSAC 8 cut(s) 43, 383, 2091, 2946, 3590, 3647, 3946, 4618
TspGWI ACGGA 3 cut(s) 31, 4210, 4626
TspMI CCCGGG 1 cut(s) 696
Van91I CCANNNNNTGG 6 cut(s) 188, 995, 1928, 1982, 2888, 5389
VneI GTGCAC 2 cut(s) 1056, 1122
VpaK11BI GGWCC 4 cut(s) 1400, 1775, 4745, 5266
VspI ATTAAT 2 cut(s) 4044, 4692
XagI CCTNNNNNAGG 6 cut(s) 2469, 3033, 3248, 3296, 4449, 4836
XbaI TCTAGA 3 cut(s) 1684, 3230, 4016
XceI RCATGY 2 cut(s) 749, 1450
XcmI CCANNNNNNNNNTGG 2 cut(s) 462, 2546
XmaI CCCGGG 1 cut(s) 696
XmaJI CCTAGG 1 cut(s) 5095
XmiI GTMKAC 1 cut(s) 954
XmnI GAANNNNTTC 4 cut(s) 561, 1367, 2958, 3538
ZrmI AGTACT 2 cut(s) 2443, 3826
Zsp2I ATGCAT 1 cut(s) 4705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.