pycom08g02740

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008668
Physical Location & Seq
Reverse (-)
1992411 .. 1998154
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02740.1

Sequence Viewer

Length: 4560 bp
ATGAACAGCAGTCCTACTATTCCTCAGTCTATAGGAACTGTATTGGACAAAGTTGAAAGTGAACATGGAGAGCCTTATCCATTAAGGTATAGTAGGACGAAGCTGCTTGATGTTTACAGGAAGGCTGATATGAAATCATATCAAAAACCAGTTGATGGGTTTATAGAAGTGAGCTCTCTTGCGGTGGATGAACCATTGGAACCTCTGGCACTTTGTGCACCGAATTCTGAGGAAATGGCTCTTTTGAAAGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCCCAGACTTCTAAAGATGGAAGGAACCCAAATGATTTTACACAGTCAAGAAGACCCAAGCTTGGCAGTAGAGAAGATTTACCCCTTGCACTTGGTGATTCTAAAGATGAAAGCATTGCTAGGTCAAAAGGTGGTCTTCCGAGTTATGCAGAGGGTTCTTCTTATGAAAGGCAGGTGCTTCATCATGGATCCAGCCTAAAAGGAGAAATAACGAAGGAACATAAAACATACTCAGAGAACAATTTCAGATCAGAAGCTTTTAGAGGAGACAGTGGTCCTTTCATAAGGGCTGAGGAGGCACCTGTTAGTGCAGAGTTGAGCATGCAGGGAGGTATTACTGCTCATTCTGGTTCTCCATGGAGAGCTTCATCACAAGGAGAACGCTCACATACAGTCTTGCATGATTGGAACGAGATTCCCGGTGATATTAAGTCAAGAACTCCTGATATGGGCTGGTCGTTAGGATCGAAAGATCTTAACAACGAATGGGAAAGTAGGGATGAGGCAAGATGGAAAACCAGTGATGATCCTGTTATAAGGAGGCAGCGATCGGGAATTCTAGATAGGGAACAAGAATTGAGACAACCTCAGCAGCTTTCTCCAGAGGAGCTTCAGCTTTATTATAAAGATCCTCAGGGTATAATTCAAGGTCCTTTCTCTGGGGCTGACATTATTGGCTGGTTTGAGGCTGGATATTTTGGCATAGATTTGCTAGTTCGCATAGCAGGTGCAAAAAATGATATGCCATTTTTGGCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGACCACCGCCTGGATTTACTGCACCTAAACCGGAGGTTGCAGATACATCAAGTAGGCCAAACTTTGGTAATGTTGGGATGATTCAAGCTGGTTTAAGTGAGATAGAGATAGCAAGAAATGATCCAAGGAATAAACAGGGCTCGACAACAGAAGCTCAGAATAGGTTTCTGGAGTCACTGATGTCTGGTAATCCGAGTGGCTCACCACTTCATAAGTTTCCTTTTTCGGAAGGTTTACAAGGATTTATAGGAAACAATCCTCACGGCTTGACTCATAGTGGATTGGATAATCTGTTGGCCAAGAGAATGGTTCTTGAACGACAGAGGTCTTTTCCTAATCCTTATCAATATTGGCCTGGGACGGATGCTTCATCTGTGATACCTATGTCGGAGATTGTCCCAGATGCAAACCCCCTCTCTTCAGTGGCTGAAAATCCTAGTCAGCCTCCTCATACCCAAAATGCTGACCTCATGTCCATCCTTCAAGGATTAACGAACCGGTCATCATCTGACATAAATAATAGTGCTGCTGGTCGGTCAACTTTTCCTGTCCAAGGTGGCTCAGAGCCACTCCAGAGTAATTTTCCTCCCCAAGCCCCACTCCAGAGTAACTTTCCTCCCCAAATCCCACTTGGGTTCCAACAGCAGAGGCTGCATCCTCAGAATCAACAATCTTTACTCTCTCAAGCTATTGATAGTTCAAGTATTTCAACGCAAGAGAAATTACTATCTTCTGGTTTATTACATGATCCACAAGTATTGAGTATGTTGCAACAGCAGTATTTATTACAGTTACATTCCCAGGCGCCTGTTCCAACACAACAAATGTCATTGTTGGATAATTTAATGTTGCTCAAGCAGCAGCAACAGAAACAGGAGGAGCAGCAAATTTTGTTAAGGCAACAACAGCAGCTGCTTTCCCAGGTTATGCCAGAGCATCACTCTCAACAGCATTTTACTGAGCCATCTTTTGGACAGATGCAGGCTTCTGTGATGCTGAAAGGGATCGATCCCTCCCGGCTTCAGTCGTCACAAGAAATGTTTCCAGTTGGTACAAATGTGCAGGTTCCTAATACGCAAAATGAACTTACTAGTAGTTATATGACTTTGCCTCCCCAAGGTAACCAAGATATCAGTCATAATGTTAGTGAGGGAGCATCCTCAGTACCTTTACCGCATCAAGTGCTTGGGAATGTTACTCATCAAAGAAGTTGGGATGTTACTCCGGCGGCACCAATTTCTATTCAGCAGGAGTCGCTGCCAATGTCCACAATTGTTGAGAGCTCACCGTTGTTTGATGTGATGACCAAATCTAGAGAGGTGTCCCTTGCGCAGGAGTCCATACCTGATTCTGATTTTCATGTTGCTAAAACCCTGGAAGAGGCAGCTGAAAACGCTTTCATAGCTGACCAATCTGCCGGGGTAGCAATTTCTCAGTGTGTTGCAGATCCTCTTCCATCATTAGGAGCTTTCAAGAAAGATGTACCTGAGCATATCAATGATGTGAATGTTCAATCAGGCAATCAAATTGAAGAACAGCAGATTGAAAGGGAAAAATACAATGATGAGGCTCCTACAGTGGTTGAAGCAAAGAATGTTGAAGTAAGAGGGCAAAGAAAGACTTCTGAGAAGAAGTCCAAGAAGCAAAAATCTGCCAAGGCACAGTCTTCAGCTGACCAAGCAAAAGGAGCATCCAAGGCGCAGTCCGAAGCTGAAAAGCCTGTTGTTGGAGATGTAAAGTTGGGGATGAGAGGCAACCGAAGCAGTAAGCCTGAAATTGTTACAGTGGAGGCTGTGGAATCCCAACAATCTGAAAAATTAGAGGCTGTGAAAGTTTCTGGAGATGATACGGAACCTCATGAAATCAGGGGTGATTCTAAGTTAGTTGAGTCTGCTTCTGGGCAAAGTACTCAAACACAGATTGGCCAGCGAGCTTGGAAGCCTGCTCCTGGTTTTAAGGCGAAATCGTTACTAGAAATTCAACAGGAAGAACAGAGGAAGGCACAGACAGAAGTGATTATACCTGAGGTCACCATCAACTCCCCCAGTTTGTCGACTCCTTGGGCCGGGGTTGTAGCCAATTCAGAACCAAAAATATCTAGAGAAACTCCGGTAGATGCAGGTATTAATGACTTAAATGCTGGGAAGCCCAAAACTTCTCAAAACCCAAAGAGCAAGAAGAGCCCGCTACATGATTTATTAGCAGATGAGGTTTTGGCCAAGTCTAGTGAAAGAGATGTCGAAATTCCTAATGGTGTGTCAACTCAGCATTCTTCACAAATTATGCCCACCTATTCAGAATCTGTAGATGGCGATAACTTTATTGAGGCAAAAGACACCAAAAAGAGTCGCAAAAGGTCAGCAAAAGCAAAGGGTACTGGAACCAAGACCTCAATATCAGTTACTGCTGTAGATGTGCCTGTTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAGCAGGAAAAAGAAGTATTGCCTACAATTCCATCAGGGCCTTCATTGGGAGATTTTGTTCTTTGGAAAGGAGAAACGGCTAACCCTGCTCCTTCTCCAGCATGGTTTACAGACTCCGGGAAGATTCCTAAACCCACATCTTTAAGAGATATCCAAAAGGAGCAGGAGAAGAGGGTTGCTGCTGCTCAGCACCAAAATCAGATTCCAACTCCACAGAAATCACAGCCACCTCAAGTCACCCGCAATAGTGCTCCGTCATGGTCGCTTTCAGCATCATCTCCATCTAAAGCTGCATCTCCCATCGCGATTAATTCCCATGCATCTCAGTCAAGAAATAAAGTAGAAGATGACTTATTCTGGGGACCAATTGATCAAGCAAAGCAAGAAAATAAGCAGGCAGATTTTCCTCATCTTGCAAGCCAGGGCAGTCGGGGAGTGAAAAATACTCCTGTAAAAGGAACTTCGGCTGGTTCATTAGGCAGGCAGAAATCAGTGGGTGGAAAGTCAACCGAGCGATTACTTTCATCCTCACCGTCTCAGTCATCTATGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCCATGGGCTTCCGAGATTGGTGCAAGAGCGAGTGCGTGAGGCTTATTGGCACGAAAGATACGAGTTTCCTTGAGTTTTGCCTGAAGCAATCCAAATCGGAGGCGGAGTTGCTTCTGAAAGAGAACCTAGGGTCGTATGATCCCGATCACGAGTTCATTGACAAATTTCTCAACTACAAGGAGTTGCTACCGGCTGATGTCCTTGAAATTGCCTTTCAAAAAGGGAATGACCAAGGAGGGGTCGCTGGGTTCAGCGGCGGAGATATGAATTCATACAGTGCAGATGTTGGGGATGTGGACCAAGACGGGTTCTCCAAAGGGGGCGGCAAGAAGAAGGGGAAGAAAGGAAAGAAGGTTAGTCCGGCTGTCTTGGGATTTAACGTCGTGAGTAACCGCATCATGATGGGTGAGATCCAGACACTGGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1520

Amino Acids

165.49

Weight (kDa)

5.9

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 302 - 337 3.4e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 821, 909
AarI CACCTGC 2 cut(s) 448, 1001
Acc16I TGCGCA 1 cut(s) 2409
Acc36I ACCTGC 4 cut(s) 448, 1001, 2131, 3188
AccB1I GGYRCC 3 cut(s) 583, 1882, 2308
AccB7I CCANNNNNTGG 5 cut(s) 155, 1088, 1142, 2048, 4549
AccI GTMKAC 1 cut(s) 3131
AccII CGCG 1 cut(s) 3848
AcoI YGGCCR 3 cut(s) 1374, 3001, 3294
AcsI RAATTY 7 cut(s) 223, 840, 1965, 3054, 3321, 4292, 4396
AcuI CTGAAG 5 cut(s) 881, 1482, 2084, 2730, 4232
AcyI GRCGYC 1 cut(s) 1883
AdeI CACNNNGTG 3 cut(s) 215, 380, 1046
AfaI GTAC 6 cut(s) 2131, 2244, 2562, 2986, 3454, 3543
AgeI ACCGGT 1 cut(s) 1575
AhdI GACNNNNNGTC 1 cut(s) 1549
AhlI ACTAGT 1 cut(s) 2168
AjnI CCWGG 7 cut(s) 1087, 1432, 1878, 1998, 2451, 3025, 3963
AjuI GAANNNNNNNTTGG 2 cut(s) 3729, 3761
AloI GAACNNNNNNTCC 2 cut(s) 3585, 3617
Alw21I GWGCWC 5 cut(s) 176, 220, 286, 2363, 3796
Alw26I GTCTC 3 cut(s) 546, 859, 4083
Alw44I GTGCAC 2 cut(s) 216, 282
AlwNI CAGNNNCTG 6 cut(s) 1505, 1729, 1990, 3380, 3482, 4549
AoxI GGCC 7 cut(s) 1133, 1374, 1430, 3001, 3141, 3294, 3581
ApaLI GTGCAC 2 cut(s) 216, 282
ApoI RAATTY 7 cut(s) 223, 840, 1965, 3054, 3321, 4292, 4396
AseI ATTAAT 2 cut(s) 3204, 3852
AsiGI ACCGGT 1 cut(s) 1575
Asp700I GAANNNNTTC 3 cut(s) 527, 2118, 2698
AspA2I CCTAGG 1 cut(s) 4255
AspLEI GCGC 3 cut(s) 1885, 2410, 2779
AspS9I GGNCC 6 cut(s) 560, 935, 3141, 3581, 3905, 4426
AsuC2I CCSGG 5 cut(s) 705, 2095, 2497, 3145, 3661
AvaII GGWCC 4 cut(s) 560, 935, 3905, 4426
AvrII CCTAGG 1 cut(s) 4255
AxyI CCTNAGG 2 cut(s) 918, 3102
BaeGI GKGCMC 2 cut(s) 220, 286
BaeI ACNNNNGTAYC 4 cut(s) 1448, 1481, 2121, 2154
BalI TGGCCA 3 cut(s) 1376, 3003, 3296
BamHI GGATCC 1 cut(s) 473
BanI GGYRCC 3 cut(s) 583, 1882, 2308
BanII GRGCYC 4 cut(s) 176, 1220, 2363, 3263
BarI GAAGNNNNNNTAC 2 cut(s) 3081, 3113
BauI CACGAG 1 cut(s) 4277
BbsI GAAGAC 3 cut(s) 343, 413, 2736
Bbv12I GWGCWC 5 cut(s) 176, 220, 286, 2363, 3796
BbvCI CCTCAGC 2 cut(s) 576, 873
BceAI ACGGC 2 cut(s) 1357, 3636
BcgI CGANNNNNNTGC 2 cut(s) 4131, 4165
BciT130I CCWGG 7 cut(s) 1089, 1434, 1880, 2000, 2453, 3027, 3965
BclI TGATCA 1 cut(s) 3913
BcnI CCSGG 5 cut(s) 705, 2095, 2497, 3145, 3661
BcoDI GTCTC 3 cut(s) 546, 859, 4083
BcuI ACTAGT 1 cut(s) 2168
BfmI CTRYAG 4 cut(s) 30, 2652, 3381, 3486
BfoI RGCGCY 1 cut(s) 1886
BfuAI ACCTGC 4 cut(s) 448, 1001, 2131, 3188
BglII AGATCT 1 cut(s) 757
BlnI CCTAGG 1 cut(s) 4255
BlpI GCTNAGC 1 cut(s) 3729
BmcAI AGTACT 1 cut(s) 2986
Bme18I GGWCC 4 cut(s) 560, 935, 3905, 4426
BmeRI GACNNNNNGTC 1 cut(s) 1549
BmgT120I GGNCC 6 cut(s) 560, 935, 3141, 3581, 3905, 4426
BmrI ACTGGG 1 cut(s) 3117
BmuI ACTGGG 1 cut(s) 3117
BoxI GACNNNNGTC 2 cut(s) 558, 1402
BpiI GAAGAC 3 cut(s) 343, 413, 2736
BplI GAGNNNNNCTC 4 cut(s) 856, 888, 2003, 2035
BpmI CTGGAG 6 cut(s) 870, 1268, 1634, 1664, 2937, 3624
Bpu10I CCTNAGC 3 cut(s) 576, 873, 2565
Bpu1102I GCTNAGC 1 cut(s) 3729
BpuEI CTTGAG 4 cut(s) 1746, 1916, 3759, 4220
BpuMI CCSGG 5 cut(s) 705, 2095, 2497, 3145, 3661
Bsa29I ATCGAT 1 cut(s) 2085
BsaBI GATNNNNATC 1 cut(s) 4272
BsaHI GRCGYC 1 cut(s) 1883
BsaWI WCCGGW 3 cut(s) 1108, 1575, 3187
Bse118I RCCGGY 2 cut(s) 1575, 4318
Bse1I ACTGG 6 cut(s) 149, 804, 2123, 3123, 3460, 4554
Bse21I CCTNAGG 2 cut(s) 918, 3102
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 4272
BseBI CCWGG 7 cut(s) 1089, 1434, 1880, 2000, 2453, 3027, 3965
BseCI ATCGAT 1 cut(s) 2085
BseJI GATNNNNATC 1 cut(s) 4272
BseMI GCAATG 1 cut(s) 399
BseNI ACTGG 6 cut(s) 149, 804, 2123, 3123, 3460, 4554
BseRI GAGGAG 5 cut(s) 564, 593, 905, 1515, 1970
BseSI GKGCMC 2 cut(s) 220, 286
BseYI CCCAGC 2 cut(s) 3218, 4373
BsgI GTGCAG 4 cut(s) 615, 1083, 2159, 4428
Bsh1236I CGCG 1 cut(s) 3848
Bsh1285I CGRYCG 1 cut(s) 836
BshFI GGCC 7 cut(s) 1135, 1376, 1432, 3003, 3143, 3296, 3583
BshNI GGYRCC 3 cut(s) 583, 1882, 2308
BshTI ACCGGT 1 cut(s) 1575
BshVI ATCGAT 1 cut(s) 2085
BsiEI CGRYCG 1 cut(s) 836
BsiHKAI GWGCWC 5 cut(s) 176, 220, 286, 2363, 3796
BslFI GGGAC 4 cut(s) 1450, 1460, 2386, 3918
BsmAI GTCTC 3 cut(s) 546, 859, 4083
BsmBI CGTCTC 1 cut(s) 4083
BsmFI GGGAC 4 cut(s) 1450, 1460, 2386, 3918
BsmI GAATGC 1 cut(s) 3346
BsnI GGCC 7 cut(s) 1135, 1376, 1432, 3003, 3143, 3296, 3583
Bsp1286I GDGCHC 7 cut(s) 176, 220, 286, 1220, 2363, 3263, 3796
Bsp1407I TGTACA 1 cut(s) 3541
Bsp1720I GCTNAGC 1 cut(s) 3729
Bsp19I CCATGG 2 cut(s) 641, 4131
Bsp68I TCGCGA 1 cut(s) 3848
BspANI GGCC 7 cut(s) 1135, 1376, 1432, 3003, 3143, 3296, 3583
BspDI ATCGAT 1 cut(s) 2085
BspFNI CGCG 1 cut(s) 3848
BspHI TCATGA 2 cut(s) 2935, 4527
BspMI ACCTGC 4 cut(s) 448, 1001, 2131, 3188
BspQI GCTCTTC 1 cut(s) 3251
BspT107I GGYRCC 3 cut(s) 583, 1882, 2308
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 2 cut(s) 1575, 4318
BsrGI TGTACA 1 cut(s) 3541
BsrI ACTGG 6 cut(s) 149, 804, 2123, 3123, 3460, 4554
BssAI RCCGGY 2 cut(s) 1575, 4318
BssNI GRCGYC 1 cut(s) 1883
BssSI CACGAG 1 cut(s) 4277
Bst2BI CACGAG 1 cut(s) 4277
Bst2UI CCWGG 7 cut(s) 1089, 1434, 1880, 2000, 2453, 3027, 3965
Bst6I CTCTTC 5 cut(s) 1501, 2451, 2535, 3251, 3707
BstACI GRCGYC 1 cut(s) 1883
BstAPI GCANNNNNTGC 3 cut(s) 215, 1729, 2260
BstAUI TGTACA 1 cut(s) 3541
BstC8I GCNNGC 9 cut(s) 608, 2061, 3005, 3009, 3021, 3263, 3939, 3961, 4025
BstDSI CCRYGG 2 cut(s) 641, 4131
BstEII GGTNACC 2 cut(s) 2198, 3106
BstENI CCTNNNNNAGG 5 cut(s) 1629, 2193, 2408, 2456, 3996
BstFNI CGCG 1 cut(s) 3848
BstH2I RGCGCY 1 cut(s) 1886
BstHHI GCGC 3 cut(s) 1885, 2410, 2779
BstMAI GTCTC 3 cut(s) 546, 859, 4083
BstMCI CGRYCG 1 cut(s) 836
BstNI CCWGG 7 cut(s) 1089, 1434, 1880, 2000, 2453, 3027, 3965
BstNSI RCATGY 1 cut(s) 610
BstPAI GACNNNNGTC 2 cut(s) 558, 1402
BstPI GGTNACC 2 cut(s) 2198, 3106
BstSFI CTRYAG 4 cut(s) 30, 2652, 3381, 3486
BstSLI GKGCMC 2 cut(s) 220, 286
BstUI CGCG 1 cut(s) 3848
BstV2I GAAGAC 3 cut(s) 343, 413, 2736
BstX2I RGATCY 5 cut(s) 473, 757, 913, 2524, 4539
BstXI CCANNNNNNTGG 1 cut(s) 1384
BstYI RGATCY 5 cut(s) 473, 757, 913, 2524, 4539
Bsu15I ATCGAT 1 cut(s) 2085
Bsu36I CCTNAGG 2 cut(s) 918, 3102
BsuRI GGCC 7 cut(s) 1135, 1376, 1432, 3003, 3143, 3296, 3583
BsuTUI ATCGAT 1 cut(s) 2085
BtgI CCRYGG 2 cut(s) 641, 4131
BtgZI GCGATG 1 cut(s) 3829
BtuMI TCGCGA 1 cut(s) 3848
BveI ACCTGC 4 cut(s) 448, 1001, 2131, 3188
Cac8I GCNNGC 9 cut(s) 608, 2061, 3005, 3009, 3021, 3263, 3939, 3961, 4025
CaiI CAGNNNCTG 6 cut(s) 1505, 1729, 1990, 3380, 3482, 4549
CciI TCATGA 2 cut(s) 2935, 4527
CfoI GCGC 3 cut(s) 1885, 2410, 2779
Cfr10I RCCGGY 2 cut(s) 1575, 4318
Cfr13I GGNCC 6 cut(s) 560, 935, 3141, 3581, 3905, 4426
ClaI ATCGAT 1 cut(s) 2085
Csp6I GTAC 6 cut(s) 2130, 2243, 2561, 2985, 3453, 3542
CspAI ACCGGT 1 cut(s) 1575
CspCI CAANNNNNGTGG 4 cut(s) 1818, 1853, 2335, 2370
CviQI GTAC 6 cut(s) 2130, 2243, 2561, 2985, 3453, 3542
DinI GGCGCC 1 cut(s) 1884
DraIII CACNNNGTG 3 cut(s) 215, 380, 1046
DriI GACNNNNNGTC 1 cut(s) 1549
EaeI YGGCCR 3 cut(s) 1374, 3001, 3294
Eam1104I CTCTTC 5 cut(s) 1501, 2451, 2535, 3251, 3707
Eam1105I GACNNNNNGTC 1 cut(s) 1549
EarI CTCTTC 5 cut(s) 1501, 2451, 2535, 3251, 3707
EciI GGCGGA 2 cut(s) 4247, 4401
Ecl136II GAGCTC 2 cut(s) 174, 2361
Eco24I GRGCYC 4 cut(s) 176, 1220, 2363, 3263
Eco32I GATATC 2 cut(s) 2209, 3694
Eco47I GGWCC 4 cut(s) 560, 935, 3905, 4426
Eco53kI GAGCTC 2 cut(s) 174, 2361
Eco57I CTGAAG 5 cut(s) 881, 1482, 2084, 2730, 4232
Eco81I CCTNAGG 2 cut(s) 918, 3102
Eco91I GGTNACC 2 cut(s) 2198, 3106
EcoICRI GAGCTC 2 cut(s) 174, 2361
EcoNI CCTNNNNNAGG 5 cut(s) 1629, 2193, 2408, 2456, 3996
EcoO109I RGGNCCY 2 cut(s) 935, 3581
EcoO65I GGTNACC 2 cut(s) 2198, 3106
EcoRI GAATTC 3 cut(s) 223, 840, 4396
EcoRII CCWGG 7 cut(s) 1087, 1432, 1878, 1998, 2451, 3025, 3963
EcoRV GATATC 2 cut(s) 2209, 3694
EcoT22I ATGCAT 1 cut(s) 3865
EcoT38I GRGCYC 4 cut(s) 176, 1220, 2363, 3263
EgeI GGCGCC 1 cut(s) 1884
EheI GGCGCC 1 cut(s) 1884
Esp3I CGTCTC 1 cut(s) 4083
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 2683, 2715
FaqI GGGAC 4 cut(s) 1450, 1460, 2386, 3918
FauI CCCGC 2 cut(s) 3270, 3791
FbaI TGATCA 1 cut(s) 3913
FblI GTMKAC 1 cut(s) 3131
FriOI GRGCYC 4 cut(s) 176, 1220, 2363, 3263
FspI TGCGCA 1 cut(s) 2409
GlaI GCGC 3 cut(s) 1884, 2409, 2778
GsaI CCCAGC 2 cut(s) 3222, 4377
GsuI CTGGAG 6 cut(s) 870, 1268, 1634, 1664, 2937, 3624
HaeII RGCGCY 1 cut(s) 1886
HaeIII GGCC 7 cut(s) 1135, 1376, 1432, 3003, 3143, 3296, 3583
HhaI GCGC 3 cut(s) 1885, 2410, 2779
Hin1I GRCGYC 1 cut(s) 1883
Hin6I GCGC 3 cut(s) 1883, 2408, 2777
HinP1I GCGC 3 cut(s) 1883, 2408, 2777
HincII GTYRAC 4 cut(s) 1617, 3132, 3339, 4050
HindII GTYRAC 4 cut(s) 1617, 3132, 3339, 4050
HindIII AAGCTT 2 cut(s) 344, 540
Hpy99I CGWCG 1 cut(s) 4514
HpyCH4IV ACGT 1 cut(s) 4509
HpySE526I ACGT 1 cut(s) 4509
Hsp92I GRCGYC 1 cut(s) 1883
HspAI GCGC 3 cut(s) 1883, 2408, 2777
KasI GGCGCC 1 cut(s) 1882
Ksp22I TGATCA 1 cut(s) 3913
LguI GCTCTTC 1 cut(s) 3251
MaeII ACGT 1 cut(s) 4509
MfeI CAATTG 2 cut(s) 2349, 3909
MflI RGATCY 5 cut(s) 473, 757, 913, 2524, 4539
MhlI GDGCHC 7 cut(s) 176, 220, 286, 1220, 2363, 3263, 3796
MlsI TGGCCA 3 cut(s) 1376, 3003, 3296
MluNI TGGCCA 3 cut(s) 1376, 3003, 3296
Mly113I GGCGCC 1 cut(s) 1883
MlyI GAGTC 8 cut(s) 1259, 1342, 2339, 2423, 2975, 3127, 3433, 3650
MmeI TCCRAC 6 cut(s) 1446, 1741, 1893, 1916, 2785, 3773
Mox20I TGGCCA 3 cut(s) 1376, 3003, 3296
Mph1103I ATGCAT 1 cut(s) 3865
MroXI GAANNNNTTC 3 cut(s) 527, 2118, 2698
MscI TGGCCA 3 cut(s) 1376, 3003, 3296
MslI CAYNNNNRTG 2 cut(s) 2574, 4529
Msp20I TGGCCA 3 cut(s) 1376, 3003, 3296
MspA1I CMGCKG 4 cut(s) 1990, 2465, 2750, 4383
MunI CAATTG 2 cut(s) 2349, 3909
Mva1269I GAATGC 1 cut(s) 3346
MvaI CCWGG 7 cut(s) 1089, 1434, 1880, 2000, 2453, 3027, 3965
MvnI CGCG 1 cut(s) 3848
NarI GGCGCC 1 cut(s) 1883
NciI CCSGG 5 cut(s) 705, 2095, 2497, 3145, 3661
NcoI CCATGG 2 cut(s) 641, 4131
NmuCI GTSAC 4 cut(s) 1251, 2106, 3106, 3778
NruI TCGCGA 1 cut(s) 3848
NsbI TGCGCA 1 cut(s) 2409
NsiI ATGCAT 1 cut(s) 3865
NspI RCATGY 1 cut(s) 610
PaeI GCATGC 1 cut(s) 610
PagI TCATGA 2 cut(s) 2935, 4527
PaqCI CACCTGC 2 cut(s) 448, 1001
PciSI GCTCTTC 1 cut(s) 3251
PcsI WCGNNNNNNNCGW 2 cut(s) 749, 4187
PctI GAATGC 1 cut(s) 3346
PdmI GAANNNNTTC 3 cut(s) 527, 2118, 2698
PflMI CCANNNNNTGG 5 cut(s) 155, 1088, 1142, 2048, 4549
PfoI TCCNGGA 1 cut(s) 3659
PinAI ACCGGT 1 cut(s) 1575
Ple19I CGATCG 1 cut(s) 836
PleI GAGTC 8 cut(s) 1258, 1342, 2338, 2422, 2974, 3127, 3432, 3650
PluTI GGCGCC 1 cut(s) 1886
PpsI GAGTC 8 cut(s) 1258, 1342, 2338, 2422, 2974, 3127, 3432, 3650
PpuMI RGGWCCY 1 cut(s) 935
PshAI GACNNNNGTC 2 cut(s) 558, 1402
PshBI ATTAAT 2 cut(s) 3204, 3852
PsiI TTATAA 2 cut(s) 821, 909
Psp124BI GAGCTC 2 cut(s) 176, 2363
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 7 cut(s) 1087, 1432, 1878, 1998, 2451, 3025, 3963
PspEI GGTNACC 2 cut(s) 2198, 3106
PspFI CCCAGC 2 cut(s) 3218, 4373
PspGI CCWGG 7 cut(s) 1087, 1432, 1878, 1998, 2451, 3025, 3963
PspPI GGNCC 6 cut(s) 560, 935, 3141, 3581, 3905, 4426
PspPPI RGGWCCY 1 cut(s) 935
PstNI CAGNNNCTG 6 cut(s) 1505, 1729, 1990, 3380, 3482, 4549
PsuI RGATCY 5 cut(s) 473, 757, 913, 2524, 4539
PvuI CGATCG 1 cut(s) 836
PvuII CAGCTG 3 cut(s) 1990, 2465, 2750
RruI TCGCGA 1 cut(s) 3848
RsaI GTAC 6 cut(s) 2131, 2244, 2562, 2986, 3454, 3543
RsaNI GTAC 6 cut(s) 2130, 2243, 2561, 2985, 3453, 3542
RseI CAYNNNNRTG 2 cut(s) 2574, 4529
SacI GAGCTC 2 cut(s) 176, 2363
SalI GTCGAC 1 cut(s) 3130
SapI GCTCTTC 1 cut(s) 3251
Sau96I GGNCC 6 cut(s) 560, 935, 3141, 3581, 3905, 4426
ScaI AGTACT 1 cut(s) 2986
SchI GAGTC 8 cut(s) 1259, 1342, 2339, 2423, 2975, 3127, 3433, 3650
SduI GDGCHC 7 cut(s) 176, 220, 286, 1220, 2363, 3263, 3796
SfcI CTRYAG 4 cut(s) 30, 2652, 3381, 3486
SfoI GGCGCC 1 cut(s) 1884
SinI GGWCC 4 cut(s) 560, 935, 3905, 4426
SmiMI CAYNNNNRTG 2 cut(s) 2574, 4529
SmlI CTYRAG 4 cut(s) 1761, 1931, 3774, 4199
SmoI CTYRAG 4 cut(s) 1761, 1931, 3774, 4199
SpeI ACTAGT 1 cut(s) 2168
SphI GCATGC 1 cut(s) 610
SspDI GGCGCC 1 cut(s) 1882
SspI AATATT 1 cut(s) 1427
SstI GAGCTC 2 cut(s) 176, 2363
TaiI ACGT 1 cut(s) 4512
TaqI TCGA 5 cut(s) 752, 1220, 2085, 3131, 3318
TaqII GACCGA 1 cut(s) 1602
TatI WGTACW 2 cut(s) 2984, 3541
TauI GCSGC 3 cut(s) 2309, 4386, 4455
TseFI GTSAC 4 cut(s) 1251, 2106, 3106, 3778
Tsp45I GTSAC 4 cut(s) 1251, 2106, 3106, 3778
TspGWI ACGGA 3 cut(s) 1454, 2942, 3786
Van91I CCANNNNNTGG 5 cut(s) 155, 1088, 1142, 2048, 4549
VneI GTGCAC 2 cut(s) 216, 282
VpaK11BI GGWCC 4 cut(s) 560, 935, 3905, 4426
VspI ATTAAT 2 cut(s) 3204, 3852
XagI CCTNNNNNAGG 5 cut(s) 1629, 2193, 2408, 2456, 3996
XapI RAATTY 7 cut(s) 223, 840, 1965, 3054, 3321, 4292, 4396
XbaI TCTAGA 3 cut(s) 844, 2390, 3176
XceI RCATGY 1 cut(s) 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 1706
XmaJI CCTAGG 1 cut(s) 4255
XmiI GTMKAC 1 cut(s) 3131
XmnI GAANNNNTTC 3 cut(s) 527, 2118, 2698
ZrmI AGTACT 1 cut(s) 2986
Zsp2I ATGCAT 1 cut(s) 3865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.