MD15G1031200.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008668
Physical Location & Seq
Reverse (-)
1900606 .. 1908450
7845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1031200.v1.1.491

Sequence Viewer

Length: 5379 bp
ATGGCCGACCTTTCCAGCTCCGCCTCCCGCAACCAGCTCTCCGTCACCACTACGCCCCAGATCTCCAAAGGTGGACAAGGTTCTGAGAATCCTATTCCCCTTTCACCGCAATGGCTTCTGCCTAAGCCGGGGGAGAGTAAGCAGGGAATGTTAACCGGGGAAAAACTTCCCAGCCCAAATCCATCCTTTGGCAGTCGCTTGGATACCATGAAATCATCAGGTAATGGTGATGAAATTCATGATACCCAGAAGAAGAAGGATGTTTTCAGGCCATCCTTGATGGATATGGAAGCTGGTGGTCGTCGTGAACGCTGGCGTGATGAAGAAAGAGACACCAACTCCTCAGGACGCAAAGATCGTTGGAGGGATGGGGATAAGGAAATTGGTGACACTCGCAGGATGGATCGACGGATGGAAAATTCATCTGCAAAGCACTTTGGGGAAGCACGTCGTGCCCCACCTGAGCGGTGGACTGACTCAAGTAACAAGGAATCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACACGCGTTGGGGGCCTGATGACAAGGAGGCAGAAGGTTTGCGTGACAAGTGGGCAGACTCTGGTAGAGATGCTAGCATGCATCTTGATAAGGGTTTGTCTCATGTTGGAAATCATGTAAAGGATGAGAAGGATGGGGATCACTACCGGCCTTGGAGATCAAATTCATCCCAAGCCCGAGGAAGGGGAGATCCTTCTCATAACCAGACAACAAACAAGAATGCTCCTATATATTCATCTAATTGGGGGCGCGGGGAAAATGCACCTCCCACCTTTTCTGTTGGTCGTGGAAGGGGTAGCTCTGGTGGAGGCTCTATGAACAGCAGTCCCACTATTCCTCAGTCTATAGGAACTGTATTGGACAAAGTTGAAAGTGAACATGGAGAGCCTTATCCATTAAGGTATAGTAGGACAAAGCTGATTGATGTATACAGGAAGGCTGATATGAAATCATATCAAAAACTAGTTGATGGGTTTATAGAAATGAGTTCTCTTATGGTGGATGAACCATTGGAGCCTCTGGCGCTTTGTGCACCGAATTCTGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCCCAGACTTCTAAAGATGGAAGGAACCCAAATGATTTTACACAGTCAAGAAGACCGAAGCTTGGCAGTAGAGAAGATATACCCCTCGCACTTAGTGACTCTAAAGATGAAAGTACTGCTAGTTCAAAAGGTGGTCTTCCTATTTATTCAGAGGGCTTTTCTTATGAAAGGCAGGTGCTCCATCATGGATCCAGCCTAAAAGCAGAAACATCGCTGGAGCAGAAACCATACTCAGAGAGCAATTTCAGAGGGGAAGCTTTTAGAGTAGACAGTGGTCCTTTCAGAAGGGTTGAGGAGGTACCTGTTGGTGCAGATTTGAGCATGCAGGGAGGTAGTACTGCTCACTTTGGCTCTCCATGGAGAGCTCCATTACAAGAGTGCTCACATACAGTCTTGCATGATTGGAAAGAGATTCCTGGTGATATCAAGTCAAGAACTCCTGATATGGGCTGGTCACTAAGACCGAAAGATCTTAATAACGAATGGGAAAGTAGGGATGAGGCAAGATGGAAAACCAGTGACGATCCAGTTATAAGGAGGCAGCCATCTGGAATTCTAGATAGGGAACAAGAATTGAGAAAACCTCAGCAGCTTTCTCCAGAGGATCTGCAGCTTTATTATAAAGATCCTCAGGGTATAATTCAAGGTCCTTTCTCTGGGGCTGACATTATTGGCTGGTTTGAGGCTGGATATTTTGGCATAGATTTGCTAGTTCGCATAGCAGGTGCATCAAATGATACGCCATTTTTGGCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGACCACCACCTGGATTTACTGCACCTAAACCCGAGGTTGCGGATACATCAAGTAGGCCAAACTTTGGTAATGTTAGGATGATGCAAGCTGGCTTAAGTGAGGCAGAGATGGCAATAAATGATCTAAGGAATAAACAGGGTTCAACAGCAGAAGCTGAGAATAGGTTTCTGGAGTCACTAATGTCTGGTAATCCGAGTGGCTCACCACTTCAGAAGTTTCCTTTTACGGAAGGTTTACAAGGATTTATGGGAAACAATCTTCATACTGGATTAGATAATCTGTTCGCCAAGAGAATGGTTCTTGAACAACAGAGGTCTTTTCCTAATCCTTATCAATATTGGCCCGGGAGAGATGTTTCATCTGTGATACCTAAGTCGGAGATTGTTGCAGATGCAAACCTCCTCTCTTCAGTGGCAGAAAGTCCTAGTCAGCCTCCTCATACCCAAAGTTCTGACATCATGTCCATCCTTCAAGGATTAACTGACCGGTCATCATTGGCCATACATAATAGTGCTGCTGGTCGGTCAACTTTTCCTGTCCAAGGTGGCTCAGACCCACTCCAGAGTAATTTGCCTCCCCAAGCCCCACTCCAGATTAACTTTCCTCCCCAGGTCCCTCTTGGATTCCAACAGCAGAGGCTGCATCCTCAGAATCAACAATCTTTACTTTCCCAAGCTATGGATAGTTCAAGTATTTCAACGCAAGAGAAATTACTTTCTTCTGGTTTATTACAAGATCCACAAGTGTTGGGCATGTTGCAACAGCAGTATTTATTGCAGTTACATTCCCAAGCGCCTGTTCCAACACAACAAATGACATTGTTGGATAAGCTCATGTTGCTGAAGCAGCAGCAACAGAAACAGGAGGAGGAGCAAAGTTTGTTAAGGCAACAACAGCAGCTGCTTTCCCAGGTTATGTCAGAGCATCAATCTCGACAGCATTTTACTGAGCCATCTTTTGGACAGATGCAGGCTTCTGCAGTGCTGAAAGGGATAGATCCTTCCTGGCTTCAGTCATCACGAGAAATGTTTCCAGTTGGTACAAATGTGCAAGTTCCCAATACACAAAATGAACTCACTAGTAGTTATATGACTTTGCCTCCCCAAGGTACCCAAGATATTAGTCATAATGTTAGTGAGGGAGCATCCTCAGTAGTGTTACCTCATCAAGTGCTTGAGAATATTACTCATCAAAGAAGTCTGGATGTTAATATGGCGGCACCAATTTCTATTCAGCAGGAGTCGTTGCCAATGTCGACAAGTGTTGAGAGCTCACCTTTGCTTGATGTGATGACCAAATCTAGAGAGGTGTCCCTTGCGCAGGACATACCTGATTCTGACTTCCATGTTGTGAAACCCCTGGAATGGGCAGCTGAAAACACTTTCATAGCTGACGGATCTGTTGGGGTTTCAATTTCTCAGGGTGTTGCAGATTCTTTGCCATCACTGGGAGCTTTCAAGAAAGATGTACATGAGTATATTAATGATGTGAATGTTCAATCAGGCAATCAAATTGAAGAACAGCGGATTGAAAGGGAAAAATGCAATGATGAGGCTCCTACAGTAGTTGAAGCAAAGAATGTTGAAGTAAGAGGGCAAAGAAAGACTTCTGAGAAGAAGTCCAAAAAGCAAAAATCTGCCAAGGTAGAGTCTTCTGGTGATCAAGCAAAAGGAGCATCCAGTGCACAGTCTGAAGCTGAAAAGCCTGTTGTTAGCGACACAAAATTGGAGACGAGAGGCAACCGAGGCAGTAAGTCTGTAATTGTTACAGCAGAGGCTTTGGAATCCCGACAAGCTGAAAATCTAGAACCTGTAAAAGTTTCTGGAGGTGATAAGGAACATAATGAATTCAGGGGTGATTCTAAGTTAGTTGAGTCTGTTTCTGGGCAAAGTACTCGAACACAGATTGGGCAGCGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCGAAATCGTTACTAGAAATTCAACAGGAAGAACAGAGGAAGGCACGGACAGAAGTGATTATACCTGAGGTCATCATCAACTCCCCCAGTTTGTCAACTCCTTGGGCTGGGGTTGTAGTCAATTCAGAACCAAAAATATCTACAGAAACTCCGGTAGATGCAGGTATTCATGACTTAAATGTTGGGAAGCCCAAAACTTCTCAAAACTCAAAGAGCAAGAAGAGCCCGCTACATGATTTATTAGCAGAGGAGGTTTTGGCCAAGTCTAGTGAAAGAGATGTTGAAATTCCTAATGGTGTATCAACTCAGCATCCTTCACAAATTATGCCCACCCGGCCAGAATCTGTAGATGACGATAACTTTATTGAGGCAAAAGACACCAAAAAAAGTCGCAAAAGGTCAGCAAAATCAAAGGGTGTTGGAACCAAGCCCTCAGTATCAGTCACTGCTGTAGATGTGCCTGTTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACATCAGGAAAATGAAGTATCGCCTGCAATTCCATCAGGGCCTTCATTGGGAGATTTTGTTCTTTGGAAAGGAGAAACAGCTAACCCTGCTCCTTCTCCAGCATGGTCTACAGACTCTGGGAAGATTTCTAAACCCACATCTTTAAGAGATATCCAAAAGGAGCAGGAGAAGAGGGTTGCTGCTACTCAGCACCAAAATCAGATTCCAACTCCTCAGAAATCACAGCCACCTCAAGTCACCCGCAGTAGTGCTCCGTCATGGTCACTTTCAGCATCATCTCCATCTAAGGCTGCATCTCCCATCGCGATTAATTCCCATGCTTCTCAGTCGAGAAATAAAGTAGAAGATGACTTATTCTGGGGCCCAATTGATCAAGCAAAGCAAGAAAATAAGCAGGCAGATTTTCCTCATCTTGCAAGCCAGGGCAGTCGGGGAGTGAAAATTACTCCTGGTAAAGGAACTTCAGCTGGTTCATTAGGCGGGCAGAAATCAGTGGGTCGAAAAGCAACTGAGCGATTACTTTCATCCTCACCGTCTCAGTCATCTATGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAGGCCATGGGCTTCCGAGATTGGTGCAAGAATGAGTGCGTTAGGCTTATTGGCACGAAAGATACGAGTTTCCTTGAGTTTTGCCTGAAGCAATCCAAATCGGAGGCTGAGTTGCTTCTGAAAGAGAACCTAGGGTCGTATGATCCCGATCACGAATTCATTGACAAATTTCTCAACTACAAGGAGTTGCTACCGGCTGATGTCCTTGAAATTGCGTTTGAAAAAGGGAATGACCAAGGGGTCGCTGGGTTGAGCGGCAGAGATGTGAATTCGTACGGTGCAGATGTTGGGGATGCGGACCAAGACGCGTCCTCCAAAGGGGTTGGCAAGAAGAAGGGAAAGAAAGGAAAGAAGGTTAGTCCGGCTGTCTTGGGATTTAACGTCGTGAGTAACCGCATCATGATGGGCGAGATCCAGACACTGGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1793

Amino Acids

196.47

Weight (kDa)

6.28

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 581 - 619 2.5e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1658, 1746
AarI CACCTGC 2 cut(s) 1288, 1838
AasI GACNNNNNNGTC 1 cut(s) 5186
Acc16I TGCGCA 1 cut(s) 3234
Acc36I ACCTGC 4 cut(s) 1288, 1838, 3850, 4010
Acc65I GGTACC 2 cut(s) 1423, 3023
AccB1I GGYRCC 3 cut(s) 1423, 3023, 3133
AccB7I CCANNNNNTGG 6 cut(s) 188, 1925, 1979, 2593, 2873, 5368
AccBSI CCGCTC 2 cut(s) 466, 5202
AccI GTMKAC 4 cut(s) 954, 1392, 3170, 4472
AccII CGCG 4 cut(s) 532, 777, 4670, 5255
AcoI YGGCCR 4 cut(s) 3, 2412, 4116, 4193
AcuI CTGAAG 7 cut(s) 2108, 2307, 2777, 2909, 3627, 4812, 5054
AdeI CACNNNGTG 3 cut(s) 452, 1220, 1883
AfaI GTAC 9 cut(s) 1240, 1425, 1462, 2956, 3025, 3384, 3808, 4365, 5222
AflII CTTAAG 1 cut(s) 2008
AflIII ACRYGT 2 cut(s) 530, 5253
AgeI ACCGGT 1 cut(s) 2400
AhdI GACNNNNNGTC 1 cut(s) 2374
AhlI ACTAGT 2 cut(s) 988, 2993
AjiI CACGTC 1 cut(s) 449
AjnI CCWGG 9 cut(s) 1540, 1924, 2523, 2823, 2918, 3273, 3847, 4785, 4813
AjuI GAANNNNNNNTTGG 4 cut(s) 2697, 2729, 4551, 4583
AloI GAACNNNNNNTCC 2 cut(s) 4407, 4439
Alw21I GWGCWC 8 cut(s) 1060, 1126, 1305, 1492, 1508, 3188, 3601, 4618
Alw26I GTCTC 4 cut(s) 324, 630, 3638, 4905
Alw44I GTGCAC 3 cut(s) 1056, 1122, 3597
AlwNI CAGNNNCTG 8 cut(s) 587, 2069, 2554, 2815, 4202, 4304, 4481, 5368
Ama87I CYCGRG 3 cut(s) 702, 1946, 2258
ApaI GGGCCC 1 cut(s) 4730
ApaLI GTGCAC 3 cut(s) 1056, 1122, 3597
AseI ATTAAT 2 cut(s) 3396, 4674
AsiGI ACCGGT 1 cut(s) 2400
Asp700I GAANNNNTTC 3 cut(s) 165, 2943, 3520
Asp718I GGTACC 2 cut(s) 1423, 3023
AspA2I CCTAGG 1 cut(s) 5077
AspLEI GCGC 4 cut(s) 777, 1051, 2710, 3235
AspS9I GGNCC 9 cut(s) 539, 1400, 1772, 2256, 2527, 4403, 4726, 4727, 5245
AsuC2I CCSGG 5 cut(s) 129, 157, 2259, 2260, 4192
AsuNHI GCTAGC 1 cut(s) 599
AvaI CYCGRG 3 cut(s) 702, 1946, 2258
AvaII GGWCC 4 cut(s) 1400, 1772, 2527, 5245
AvrII CCTAGG 1 cut(s) 5077
AxyI CCTNAGG 3 cut(s) 343, 1755, 3924
BaeGI GKGCMC 5 cut(s) 457, 1060, 1126, 3601, 4730
BaeI ACNNNNGTAYC 4 cut(s) 2273, 2306, 2946, 2979
BalI TGGCCA 2 cut(s) 2414, 4118
BamHI GGATCC 1 cut(s) 1313
BanI GGYRCC 3 cut(s) 1423, 3023, 3133
BanII GRGCYC 4 cut(s) 1492, 3188, 4085, 4730
BarI GAAGNNNNNNTAC 4 cut(s) 3903, 3935, 4810, 4842
BauI CACGAG 1 cut(s) 2934
BbsI GAAGAC 3 cut(s) 1183, 1253, 3558
Bbv12I GWGCWC 8 cut(s) 1060, 1126, 1305, 1492, 1508, 3188, 3601, 4618
BbvCI CCTCAGC 1 cut(s) 1710
BceAI ACGGC 1 cut(s) 524
BcgI CGANNNNNNTGC 8 cut(s) 1317, 1351, 2828, 2862, 3791, 3825, 4953, 4987
BciT130I CCWGG 9 cut(s) 1542, 1926, 2525, 2825, 2920, 3275, 3849, 4787, 4815
BciVI GTATCC 2 cut(s) 196, 1951
BclI TGATCA 2 cut(s) 3574, 4735
BcnI CCSGG 5 cut(s) 129, 157, 2259, 2260, 4192
BcoDI GTCTC 4 cut(s) 324, 630, 3638, 4905
BcuI ACTAGT 2 cut(s) 988, 2993
BfmI CTRYAG 8 cut(s) 870, 1733, 2892, 3474, 3999, 4203, 4308, 4473
BfoI RGCGCY 2 cut(s) 1052, 2711
BfrI CTTAAG 1 cut(s) 2008
BfuAI ACCTGC 4 cut(s) 1288, 1838, 3850, 4010
BfuI GTATCC 2 cut(s) 196, 1951
BglI GCCNNNNNGGC 1 cut(s) 4192
BglII AGATCT 2 cut(s) 60, 1594
BlnI CCTAGG 1 cut(s) 5077
BmcAI AGTACT 3 cut(s) 1240, 1462, 3808
Bme18I GGWCC 4 cut(s) 1400, 1772, 2527, 5245
BmeRI GACNNNNNGTC 1 cut(s) 2374
BmeT110I CYCGRG 3 cut(s) 702, 1946, 2258
BmgBI CACGTC 1 cut(s) 449
BmgT120I GGNCC 9 cut(s) 539, 1400, 1772, 2256, 2527, 4403, 4726, 4727, 5245
BmrI ACTGGG 2 cut(s) 3371, 3939
BmtI GCTAGC 1 cut(s) 603
BmuI ACTGGG 2 cut(s) 3371, 3939
BoxI GACNNNNGTC 1 cut(s) 1398
BpiI GAAGAC 3 cut(s) 1183, 1253, 3558
BplI GAGNNNNNCTC 2 cut(s) 1693, 1725
BpmI CTGGAG 7 cut(s) 1361, 1707, 2105, 2459, 2489, 3759, 4446
Bpu10I CCTNAGC 3 cut(s) 123, 462, 1710
BpuEI CTTGAG 4 cut(s) 463, 3110, 4581, 5042
BpuMI CCSGG 5 cut(s) 129, 157, 2259, 2260, 4192
BsaBI GATNNNNATC 2 cut(s) 663, 5094
BsaWI WCCGGW 2 cut(s) 2400, 4009
Bse118I RCCGGY 3 cut(s) 672, 2400, 5140
Bse1I ACTGG 8 cut(s) 1641, 1652, 2185, 2948, 3366, 3594, 3945, 5373
Bse21I CCTNAGG 3 cut(s) 343, 1755, 3924
Bse3DI GCAATG 2 cut(s) 116, 3466
Bse8I GATNNNNATC 2 cut(s) 663, 5094
BseBI CCWGG 9 cut(s) 1542, 1926, 2525, 2825, 2920, 3275, 3849, 4787, 4815
BseJI GATNNNNATC 2 cut(s) 663, 5094
BseMI GCAATG 2 cut(s) 116, 3466
BseNI ACTGG 8 cut(s) 1641, 1652, 2185, 2948, 3366, 3594, 3945, 5373
BseRI GAGGAG 8 cut(s) 331, 1433, 2306, 2340, 2795, 2798, 4121, 4566
BseSI GKGCMC 5 cut(s) 457, 1060, 1126, 3601, 4730
BseYI CCCAGC 3 cut(s) 170, 3965, 5192
BsgI GTGCAG 3 cut(s) 1455, 1920, 5247
Bsh1236I CGCG 4 cut(s) 532, 777, 4670, 5255
BshNI GGYRCC 3 cut(s) 1423, 3023, 3133
BshTI ACCGGT 1 cut(s) 2400
BsiHKAI GWGCWC 8 cut(s) 1060, 1126, 1305, 1492, 1508, 3188, 3601, 4618
BsiHKCI CYCGRG 3 cut(s) 702, 1946, 2258
BsiSI CCGG 9 cut(s) 128, 156, 673, 2259, 2401, 4010, 4192, 5141, 5309
BsiWI CGTACG 1 cut(s) 5220
BslFI GGGAC 3 cut(s) 837, 2513, 3211
BsmAI GTCTC 4 cut(s) 324, 630, 3638, 4905
BsmBI CGTCTC 2 cut(s) 3638, 4905
BsmFI GGGAC 3 cut(s) 837, 2513, 3211
BsmI GAATGC 1 cut(s) 751
BsoBI CYCGRG 3 cut(s) 702, 1946, 2258
Bsp120I GGGCCC 1 cut(s) 4726
Bsp1407I TGTACA 2 cut(s) 3382, 4363
Bsp19I CCATGG 2 cut(s) 1481, 4953
Bsp68I TCGCGA 1 cut(s) 4670
BspFNI CGCG 4 cut(s) 532, 777, 4670, 5255
BspHI TCATGA 3 cut(s) 238, 4027, 5346
BspMAI CTGCAG 2 cut(s) 1737, 2896
BspMI ACCTGC 4 cut(s) 1288, 1838, 3850, 4010
BspOI GCTAGC 1 cut(s) 603
BspQI GCTCTTC 1 cut(s) 4073
BspT107I GGYRCC 3 cut(s) 1423, 3023, 3133
BspTI CTTAAG 1 cut(s) 2008
BsrBI CCGCTC 2 cut(s) 466, 5202
BsrDI GCAATG 2 cut(s) 116, 3466
BsrFI RCCGGY 3 cut(s) 672, 2400, 5140
BsrGI TGTACA 2 cut(s) 3382, 4363
BsrI ACTGG 8 cut(s) 1641, 1652, 2185, 2948, 3366, 3594, 3945, 5373
BssAI RCCGGY 3 cut(s) 672, 2400, 5140
BssNAI GTATAC 1 cut(s) 955
BssSI CACGAG 1 cut(s) 2934
BssT1I CCWWGG 9 cut(s) 677, 1481, 2455, 3019, 3555, 3959, 4953, 5077, 5182
Bst1107I GTATAC 1 cut(s) 955
Bst2BI CACGAG 1 cut(s) 2934
Bst2UI CCWGG 9 cut(s) 1542, 1926, 2525, 2825, 2920, 3275, 3849, 4787, 4815
Bst4CI ACNGT 8 cut(s) 880, 1170, 1397, 1516, 3478, 3603, 4899, 5225
Bst6I CTCTTC 3 cut(s) 2326, 4073, 4529
BstAFI CTTAAG 1 cut(s) 2008
BstAPI GCANNNNNTGC 3 cut(s) 452, 2554, 3596
BstAUI TGTACA 2 cut(s) 3382, 4363
BstDSI CCRYGG 2 cut(s) 1481, 4953
BstENI CCTNNNNNAGG 4 cut(s) 2454, 3018, 3233, 4818
BstFNI CGCG 4 cut(s) 532, 777, 4670, 5255
BstH2I RGCGCY 2 cut(s) 1052, 2711
BstHHI GCGC 4 cut(s) 777, 1051, 2710, 3235
BstMAI GTCTC 4 cut(s) 324, 630, 3638, 4905
BstNI CCWGG 9 cut(s) 1542, 1926, 2525, 2825, 2920, 3275, 3849, 4787, 4815
BstNSI RCATGY 3 cut(s) 607, 1450, 2671
BstPAI GACNNNNGTC 1 cut(s) 1398
BstSFI CTRYAG 8 cut(s) 870, 1733, 2892, 3474, 3999, 4203, 4308, 4473
BstSLI GKGCMC 5 cut(s) 457, 1060, 1126, 3601, 4730
BstUI CGCG 4 cut(s) 532, 777, 4670, 5255
BstV2I GAAGAC 3 cut(s) 1183, 1253, 3558
BstXI CCANNNNNNTGG 1 cut(s) 2209
BstZ17I GTATAC 1 cut(s) 955
Bsu36I CCTNAGG 3 cut(s) 343, 1755, 3924
BsuI GTATCC 2 cut(s) 196, 1951
BtgI CCRYGG 2 cut(s) 1481, 4953
BtgZI GCGATG 2 cut(s) 1320, 4651
BtrI CACGTC 1 cut(s) 449
BtsI GCAGTG 2 cut(s) 2901, 4302
BtuMI TCGCGA 1 cut(s) 4670
BveI ACCTGC 4 cut(s) 1288, 1838, 3850, 4010
CaiI CAGNNNCTG 8 cut(s) 587, 2069, 2554, 2815, 4202, 4304, 4481, 5368
CciI TCATGA 3 cut(s) 238, 4027, 5346
CfoI GCGC 4 cut(s) 777, 1051, 2710, 3235
Cfr10I RCCGGY 3 cut(s) 672, 2400, 5140
Cfr13I GGNCC 9 cut(s) 539, 1400, 1772, 2256, 2527, 4403, 4726, 4727, 5245
Cfr9I CCCGGG 1 cut(s) 2258
CseI GACGC 3 cut(s) 357, 5244, 5261
Csp6I GTAC 9 cut(s) 1239, 1424, 1461, 2955, 3024, 3383, 3807, 4364, 5221
CspAI ACCGGT 1 cut(s) 2400
CspCI CAANNNNNGTGG 2 cut(s) 2643, 2678
CviQI GTAC 9 cut(s) 1239, 1424, 1461, 2955, 3024, 3383, 3807, 4364, 5221
DraIII CACNNNGTG 3 cut(s) 452, 1220, 1883
DrdI GACNNNNNNGTC 1 cut(s) 5186
DriI GACNNNNNGTC 1 cut(s) 2374
DseDI GACNNNNNNGTC 1 cut(s) 5186
EaeI YGGCCR 4 cut(s) 3, 2412, 4116, 4193
Eam1104I CTCTTC 3 cut(s) 2326, 4073, 4529
Eam1105I GACNNNNNGTC 1 cut(s) 2374
EarI CTCTTC 3 cut(s) 2326, 4073, 4529
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 2 cut(s) 1490, 3186
Eco130I CCWWGG 9 cut(s) 677, 1481, 2455, 3019, 3555, 3959, 4953, 5077, 5182
Eco24I GRGCYC 4 cut(s) 1492, 3188, 4085, 4730
Eco32I GATATC 2 cut(s) 1549, 4516
Eco47I GGWCC 4 cut(s) 1400, 1772, 2527, 5245
Eco53kI GAGCTC 2 cut(s) 1490, 3186
Eco57I CTGAAG 7 cut(s) 2108, 2307, 2777, 2909, 3627, 4812, 5054
Eco81I CCTNAGG 3 cut(s) 343, 1755, 3924
Eco88I CYCGRG 3 cut(s) 702, 1946, 2258
EcoICRI GAGCTC 2 cut(s) 1490, 3186
EcoNI CCTNNNNNAGG 4 cut(s) 2454, 3018, 3233, 4818
EcoO109I RGGNCCY 5 cut(s) 539, 1772, 2527, 4403, 4726
EcoRI GAATTC 5 cut(s) 1063, 1677, 3761, 5102, 5215
EcoRII CCWGG 9 cut(s) 1540, 1924, 2523, 2823, 2918, 3273, 3847, 4785, 4813
EcoRV GATATC 2 cut(s) 1549, 4516
EcoT14I CCWWGG 9 cut(s) 677, 1481, 2455, 3019, 3555, 3959, 4953, 5077, 5182
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 4 cut(s) 1492, 3188, 4085, 4730
ErhI CCWWGG 9 cut(s) 677, 1481, 2455, 3019, 3555, 3959, 4953, 5077, 5182
Esp3I CGTCTC 2 cut(s) 3638, 4905
FalI AAGNNNNNCTT 4 cut(s) 1245, 1277, 3505, 3537
FaqI GGGAC 3 cut(s) 837, 2513, 3211
FauI CCCGC 5 cut(s) 35, 770, 4092, 4613, 4838
FbaI TGATCA 2 cut(s) 3574, 4735
FblI GTMKAC 4 cut(s) 954, 1392, 3170, 4472
FriOI GRGCYC 4 cut(s) 1492, 3188, 4085, 4730
FspI TGCGCA 1 cut(s) 3234
GlaI GCGC 4 cut(s) 776, 1050, 2709, 3234
GsaI CCCAGC 3 cut(s) 174, 3969, 5196
GsuI CTGGAG 7 cut(s) 1361, 1707, 2105, 2459, 2489, 3759, 4446
HaeII RGCGCY 2 cut(s) 1052, 2711
HapII CCGG 9 cut(s) 128, 156, 673, 2259, 2401, 4010, 4192, 5141, 5309
HgaI GACGC 3 cut(s) 357, 5244, 5261
HhaI GCGC 4 cut(s) 777, 1051, 2710, 3235
Hin6I GCGC 4 cut(s) 775, 1049, 2708, 3233
HinP1I GCGC 4 cut(s) 775, 1049, 2708, 3233
HincII GTYRAC 4 cut(s) 153, 2442, 3171, 3954
HindII GTYRAC 4 cut(s) 153, 2442, 3171, 3954
HindIII AAGCTT 2 cut(s) 1184, 1380
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 9 cut(s) 128, 156, 673, 2259, 2401, 4010, 4192, 5141, 5309
Hpy99I CGWCG 4 cut(s) 306, 411, 453, 5333
HpyCH4III ACNGT 8 cut(s) 880, 1170, 1397, 1516, 3478, 3603, 4899, 5225
HpyCH4IV ACGT 2 cut(s) 448, 5328
HpySE526I ACGT 2 cut(s) 448, 5328
HspAI GCGC 4 cut(s) 775, 1049, 2708, 3233
KpnI GGTACC 2 cut(s) 1427, 3027
Ksp22I TGATCA 2 cut(s) 3574, 4735
KspAI GTTAAC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 4073
MaeII ACGT 2 cut(s) 448, 5328
MbiI CCGCTC 2 cut(s) 466, 5202
MfeI CAATTG 1 cut(s) 4731
MlsI TGGCCA 2 cut(s) 2414, 4118
MluI ACGCGT 2 cut(s) 530, 5253
MluNI TGGCCA 2 cut(s) 2414, 4118
MlyI GAGTC 8 cut(s) 470, 578, 1217, 2096, 3164, 3572, 3797, 4472
MmeI TCCRAC 9 cut(s) 341, 613, 2271, 2566, 2718, 2741, 3815, 4258, 4595
Mox20I TGGCCA 2 cut(s) 2414, 4118
Mph1103I ATGCAT 1 cut(s) 609
MroXI GAANNNNTTC 3 cut(s) 165, 2943, 3520
MscI TGGCCA 2 cut(s) 2414, 4118
MslI CAYNNNNRTG 3 cut(s) 109, 2424, 5348
Msp20I TGGCCA 2 cut(s) 2414, 4118
MspA1I CMGCKG 4 cut(s) 2815, 3287, 3439, 4832
MspCI CTTAAG 1 cut(s) 2008
MspI CCGG 9 cut(s) 128, 156, 673, 2259, 2401, 4010, 4192, 5141, 5309
MunI CAATTG 1 cut(s) 4731
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 9 cut(s) 1542, 1926, 2525, 2825, 2920, 3275, 3849, 4787, 4815
MvnI CGCG 4 cut(s) 532, 777, 4670, 5255
NciI CCSGG 5 cut(s) 129, 157, 2259, 2260, 4192
NcoI CCATGG 2 cut(s) 1481, 4953
NheI GCTAGC 1 cut(s) 599
NruI TCGCGA 1 cut(s) 4670
NsbI TGCGCA 1 cut(s) 3234
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 3 cut(s) 607, 1450, 2671
PaeI GCATGC 2 cut(s) 607, 1450
PagI TCATGA 3 cut(s) 238, 4027, 5346
PaqCI CACCTGC 2 cut(s) 1288, 1838
PciSI GCTCTTC 1 cut(s) 4073
PcsI WCGNNNNNNNCGW 2 cut(s) 355, 5009
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 3 cut(s) 165, 2943, 3520
Pfl23II CGTACG 1 cut(s) 5220
PflMI CCANNNNNTGG 6 cut(s) 188, 1925, 1979, 2593, 2873, 5368
PinAI ACCGGT 1 cut(s) 2400
PleI GAGTC 8 cut(s) 470, 578, 1217, 2095, 3163, 3571, 3796, 4472
PpsI GAGTC 8 cut(s) 470, 578, 1217, 2095, 3163, 3571, 3796, 4472
PpuMI RGGWCCY 2 cut(s) 1772, 2527
PshAI GACNNNNGTC 1 cut(s) 1398
PshBI ATTAAT 2 cut(s) 3396, 4674
PsiI TTATAA 2 cut(s) 1658, 1746
Psp124BI GAGCTC 2 cut(s) 1492, 3188
Psp5II RGGWCCY 2 cut(s) 1772, 2527
Psp6I CCWGG 9 cut(s) 1540, 1924, 2523, 2823, 2918, 3273, 3847, 4785, 4813
PspFI CCCAGC 3 cut(s) 170, 3965, 5192
PspGI CCWGG 9 cut(s) 1540, 1924, 2523, 2823, 2918, 3273, 3847, 4785, 4813
PspLI CGTACG 1 cut(s) 5220
PspOMI GGGCCC 1 cut(s) 4726
PspPI GGNCC 9 cut(s) 539, 1400, 1772, 2256, 2527, 4403, 4726, 4727, 5245
PspPPI RGGWCCY 2 cut(s) 1772, 2527
PsrI GAACNNNNNNTAC 2 cut(s) 1231, 1263
PstI CTGCAG 2 cut(s) 1737, 2896
PstNI CAGNNNCTG 8 cut(s) 587, 2069, 2554, 2815, 4202, 4304, 4481, 5368
PvuII CAGCTG 3 cut(s) 2815, 3287, 4832
RruI TCGCGA 1 cut(s) 4670
RsaI GTAC 9 cut(s) 1240, 1425, 1462, 2956, 3025, 3384, 3808, 4365, 5222
RsaNI GTAC 9 cut(s) 1239, 1424, 1461, 2955, 3024, 3383, 3807, 4364, 5221
RseI CAYNNNNRTG 3 cut(s) 109, 2424, 5348
SacI GAGCTC 2 cut(s) 1492, 3188
SalI GTCGAC 1 cut(s) 3169
SapI GCTCTTC 1 cut(s) 4073
Sau96I GGNCC 9 cut(s) 539, 1400, 1772, 2256, 2527, 4403, 4726, 4727, 5245
ScaI AGTACT 3 cut(s) 1240, 1462, 3808
SchI GAGTC 8 cut(s) 470, 578, 1217, 2096, 3164, 3572, 3797, 4472
SfcI CTRYAG 8 cut(s) 870, 1733, 2892, 3474, 3999, 4203, 4308, 4473
SinI GGWCC 4 cut(s) 1400, 1772, 2527, 5245
SmaI CCCGGG 1 cut(s) 2260
SmiMI CAYNNNNRTG 3 cut(s) 109, 2424, 5348
SmlI CTYRAG 5 cut(s) 478, 2008, 3089, 4596, 5021
SmoI CTYRAG 5 cut(s) 478, 2008, 3089, 4596, 5021
SpeI ACTAGT 2 cut(s) 988, 2993
SphI GCATGC 2 cut(s) 607, 1450
SspI AATATT 2 cut(s) 2252, 3097
SstI GAGCTC 2 cut(s) 1492, 3188
StyI CCWWGG 9 cut(s) 677, 1481, 2455, 3019, 3555, 3959, 4953, 5077, 5182
TaaI ACNGT 8 cut(s) 880, 1170, 1397, 1516, 3478, 3603, 4899, 5225
TaiI ACGT 2 cut(s) 451, 5331
TaqI TCGA 6 cut(s) 406, 2848, 3170, 3811, 4694, 4864
TaqII GACCGA 3 cut(s) 1195, 1603, 2427
TatI WGTACW 5 cut(s) 1238, 1460, 3382, 3806, 4363
TauI GCSGC 2 cut(s) 3134, 5205
TspGWI ACGGA 6 cut(s) 31, 424, 2156, 3324, 3919, 4608
TspMI CCCGGG 1 cut(s) 2258
Van91I CCANNNNNTGG 6 cut(s) 188, 1925, 1979, 2593, 2873, 5368
Vha464I CTTAAG 1 cut(s) 2008
VneI GTGCAC 3 cut(s) 1056, 1122, 3597
VpaK11BI GGWCC 4 cut(s) 1400, 1772, 2527, 5245
VspI ATTAAT 2 cut(s) 3396, 4674
XagI CCTNNNNNAGG 4 cut(s) 2454, 3018, 3233, 4818
XbaI TCTAGA 3 cut(s) 1681, 3215, 3718
XceI RCATGY 3 cut(s) 607, 1450, 2671
XcmI CCANNNNNNNNNTGG 3 cut(s) 465, 2531, 5189
XmaI CCCGGG 1 cut(s) 2258
XmaJI CCTAGG 1 cut(s) 5077
XmiI GTMKAC 4 cut(s) 954, 1392, 3170, 4472
XmnI GAANNNNTTC 3 cut(s) 165, 2943, 3520
ZrmI AGTACT 3 cut(s) 1240, 1462, 3808
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.