FvH4_2g39920

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008668
Physical Location & Seq
Forward (+)
28529946 .. 28539346
9401 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39920.t4

Sequence Viewer

Length: 5274 bp
ATGGCCGACATCACCGACTCCGGTTCCCGCCACCACCTCTCCGTCACCACTCCCCCCCAGATCTCCAAAGCTGGACAAGGTTCTGAGAATCCTATCCCACTGTCACCGCAATGGCTTCTGCCAAAGCCGGGGGAGAATAAGCCTGGGGCGTTAAGTGGGATGCAGGAAAAACCTCTCAGCCCAAACCCATCATTTGGGAATCGCTCAGACACCATGAAATTGTCAGGAAATGGTGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGATGGACATGGAAACTGGTGGTCGTCGTGAACGCTGGCGGGATGAAGAAAGAGACACCAATTCTGCTGTGCGGAAGGATTGGTGGAGGGATGGGGACAAAGAACTTAATGACACTCGCAGGATGGATCGTCGTACGGAAAACACACCAACAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACCGACTCAAGTAACAAGGAGTCCAACTATGAGCAACGACGGGAGAGCAAATGGAACTCGCGTTGGGGTCCTGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCAAAGATGGGAGCATGCCGGATAAAGGCTCATCTCATGTTGGAATTCACGGAAAGGATGAGAAGGATGGGGATCACTACCGGCCTTGGAGATCAAACTCTTCCCAAATTCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCGCCAGTCAACAAATATATTCCAGGTAGGGGCCGTGGGGAAAGTACACCTCCAACCTTTTCTGTTGGCCGTGGAAGAGTTGGCCCTGGTGGAAGCTGTATGAGCAGTGTTCCTACAATTTCTCAATCTGTAGGAATTTTGGACAAAGTTGAAATTGAACATGGAGAGTCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAGGACGGCTGATATGAGATCGTATCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACACTGGGGGAACCGTTGGAGCCTCTGGCTCTTTGTGCACCAAATTCTGAAGAAATGGCTCTTCTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGATTTTACACAAACAAGAAGAACAAATCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGAGTCTAAGGATGAACACATTGTTAGTTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACATCATCATGGGTCGAGCCTAAAAGCCGAGACAACGCTGGACCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGAGACGATGGAGGTCCTTTCAGAAAAGCTGACGAGCCACCTAGTAGCAGGGAGTTGAGCATGTCGGGGGGAGTGACTGCTCATGCTGGCACTCCATGGAGAGCTCCATCACAGGTAGAGCGCTCAAATACGGTCTTTCATGATTGGCAAGACACTCCCAGAGATATGAAGTCAGGCACGCCCCCTGTTATGACGTGGTCACAGAGACAGAAAGATCTAAATAATGACTGGGAAAGTAATCTGGCCGATCAATCTTACACCAGAAATGACGCGAAATGGAAAACCAGTGAGGATCCTATTATTAGAAGGCAGCTATCTGGAGTTCTAGATAGGGAACAGGAAGTGAGAAAACCTCAGCAACCTTTACCGGAGGAACTCCAACTTTATTATAAGGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGACGATATTATTGGCTGGTTTGAAGCCGGATATTTTGGTATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGAGTCACCATTTTCAGCACTTGGTGACGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTCAAGTAGGTCAAACTTTGGTAATGTGGGGAAGATTCATACTGGTTTAAGTGAGGCAGATATAATAAGAACTGAACCAAGGCTTAAGCAGACTTCAATGACTGAAGCTGAAAATAGGTTTTTGGAGTCACTGATGTCTGGTAATACAAGTGGTTCTACTCATCAGCAGTTCCCTTTCTCTGAAGGTTTGCAAGGTTTTGTTGGAAATAATTCTCATGGTTTGCCTTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCGCTTGAAAGACAGAGGTCTATACCTAATCCTTATCTTGAAAATCCCCATATCCAAAATGTTGAGGTCAATTCAGTCCTTCAAGGATTAACTGATAGGTCATCTGGTATAAATAATAATGCTGCTGGTTGGTCAAGTTTTCCTGGTCAAGGTGGGTCAGATCCACTGCAGAGTAAGATAGACATGTATCATGATCAAAGTTTTCCTCCCCAAGCTCCTCTTGGTTTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCCTTTCCGAATCTACTATCCCAAGCTGTTGACAGTTCATCAACACAAGAGAAATTGCTCTCTTCTGGCTTATTGCAAGATCCCCAGCTGATGAATATATTGCAACAGCAATACTTGATGCAGTTGCATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCGTTATTGGAGAAGATGGTGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTGTTAATGAGGCAACAACAGCAGCTCCTTTCCCAGGTTCTTGCGGAGCATCAGTCCCGCCAAAATTTTTCTGAGCCGTCGTTCGGACAGTTACAAGCTACTGCCATTCTGAAAGGGAATGCATCTATAGATCCGTCTCGGCTTCAGGCATCACAAGAAATGTTTTCACTTGGTACAAATGTGTCAGTTCCTAATATGCAGAATGAGCTCACTACTAACTTTATGGGTCTGCCTCCTCAAGGCACTCAAGATATTCGTCATCATGTAAGTGATGGGACCCCTTCTTTACCTCTATCACATCAGGTCTTTGGGAATATTATTCATCAAAGAAGTTGGGATAGTACTCATGACAGACCATCTAATGATATCCATCAAGATTCGTTGCCTGTGTCAAATATTGCTGAGAGGTCATCCTTGCTTGAGGGGACCCGTGTGCATAATTCCATCCCGGATTCTGACTTCAATGGTGCTAGAACTGTGGAACAGGCTTCAGAAAAAACTTCCAGGGATGCGGCAACTGAGGTTGTTTCTGAGACTGTTGCAGATTCCGCATCTTTAAAGTCTCCTCGAAGTTTTATTTCTATGCCACCAGGAGCTTGTGAGGAAGATATGCGTGAGCATGCCAATGATGGGAAACCTCAGTTTGATAGTCAGGTTGAAGAGCAGGTGGTTGAAAAGGAAAAAGGCAATGATGAGGCTACTTTGGTGTCTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAGAAGACATCTGAGAAGAAGTCGAAGAAGCAAAAAGCTTCCAAGGCACAGTACACAAGTGACCAGGCAAAAGGAGTATCAAAATCTGTCTCTTCACAGCAAATAGAGCAGTCCGAAACTGACCTCATTTCAGGAATTGGTACTTCAGAGGCTGTGCAATCCCAACAAGCTGGAGGGGATACTGGATATCTTCAAGTCAATGTTGACTCTAAACCAGTTGATCCTGTTGCTGTGCAGAATACCCAAGTACCTGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGCCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAGAGCGCAAACTGAAGTTGTAGTATCTGAAGTCCCCAATTCAGTCAACTCACCAGGTTTGTCAACTCCTTGGGCTGGAGTTGTGGCCAATTCAGACCCAAAAATATCTAGAGAGAATGAGAGAGATGCAGAGATTAATGAATTAAATGTTGGGAAACCTGGATCTTCAAATCGTAAGAGCAAGAAGAGCCCGTTACATGATCTTTTGACAGAGGAGGTTCTGTCCAAAGCTAGTGCAGTCATCGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCGCAAGTTATGCCTCATTCAGTACCTGTGGATGATGATAACTTCATCGAGGCTAAAGACACCAAAAGGAGTCGCAAGAAGTCGGCAAAATCAAAGGGTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGCAGAACTGCCTATTAGTTCGAGCCCTACTGAGAAAGTCAAGAGCTCCCGTTCTGTACAACAGGAAAAGGAAGTATTACCCACAATTCCTTCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCGAACGCTGCTCCATCTCCAGCATGGTCTACCGACTCTGGGAAGCTTAATAAACCAACATCTTTAAGAGATATCCAAAAGGAGCAACAGAAGAGGGTTTCCTCTGCTCAGCACGTGAACCAGATTACAGCTCCTCAGAAATCCCAGCCAACTCAAGCCACTCGCAACAGTACTCCTTCATGGTCACTTTCAGGATCATCTCCATCTAAACCTGCTTCTCCAATCCAGATCAATTCCCATGCTTCTCAGTCAAAGTATAAAGGAGACGATGACCTATTCTGGGGCCCAATCAACCAATCAAAGCAAGAAGCCAAACAAGCTGATTTTCCTCAGCTGGCAAGCCAGGGCAGTAGGGGAATGAAAAGTACTCCTGCAAAAGTAAATTCAGCTGGTTCATTAAGCCGGCAGAAATCTACAGTTGGCAAAGAAACTGAGCGATTGCTTTCATCCTCTGCGGCCCCTGCTCAGTCATCTGTGAAGGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGTTAGGCTAATTGGGACAAAAGATACGAGTGTCCTCGAATTTTGTTTGAAGCAGTCTAGATCTGAGGCTGAGTTGCTTCTGATAGAGAATCTTGGGTCTTACGATCCTGATCACAAGTTCATTGAAGAATTTCTCAACTACAAGGAGTTGCTACCAGCTGATGTCCTGGAAATAGCTTTCCAAAGTCGAGATGATCAAAAGGCCACTGGATTCAGTGGTGTCAATTCCTACAGTGCAAATGCTGGGGATGTTGATCAAGATGGTGGGTCATCCAAAGGGGGTGGCAAGAAGAAGGGGAAGAAAGGGAAAAAGGTTAGCCCTGCTGTCTTGGGCTTTAATGTAGTTAGTAACCGTATCATGATGGGTGAGATCCAGACAGTTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1758

Amino Acids

193.11

Weight (kDa)

6.36

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 536 - 573 4.6e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1770
AarI CACCTGC 1 cut(s) 3376
Acc36I ACCTGC 3 cut(s) 3376, 3745, 4555
AccB1I GGYRCC 1 cut(s) 2641
AccB7I CCANNNNNTGG 2 cut(s) 194, 1949
AccI GTMKAC 2 cut(s) 948, 4364
AccII CGCG 2 cut(s) 538, 1652
AcoI YGGCCR 5 cut(s) 3, 805, 1623, 2232, 3876
AcvI CACGTG 1 cut(s) 4450
AdeI CACNNNGTG 2 cut(s) 458, 1907
AfeI AGCGCT 1 cut(s) 1502
AflII CTTAAG 1 cut(s) 2069
AflIII ACRYGT 1 cut(s) 2427
AjiI CACGTC 2 cut(s) 455, 1575
AjuI GAANNNNNNNTTGG 2 cut(s) 3924, 3956
AloI GAACNNNNNNTCC 2 cut(s) 4299, 4331
Alw21I GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1486, 1879, 1963, 2931, 4247
Alw44I GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
AlwNI CAGNNNCTG 2 cut(s) 3614, 4094
Aor51HI AGCGCT 1 cut(s) 1502
ApaI GGGCCC 1 cut(s) 4622
ApaLI GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
ApeKI GCWGC 7 cut(s) 937, 1690, 2366, 2695, 2698, 2743, 4343
ArsI GACNNNNNNTTYG 2 cut(s) 1202, 1234
AseI ATTAAT 1 cut(s) 3927
Asp700I GAANNNNTTC 3 cut(s) 1361, 2194, 5013
AspLEI GCGC 3 cut(s) 1503, 2248, 3799
AsuC2I CCSGG 2 cut(s) 129, 3170
AsuHPI GGTGA 9 cut(s) 4, 37, 96, 245, 1881, 1919, 3834, 4186, 5261
AsuII TTCGAA 1 cut(s) 1259
AvaII GGWCC 6 cut(s) 477, 545, 1336, 1394, 2997, 3147
BaeGI GKGCMC 6 cut(s) 463, 1054, 1120, 1879, 1963, 4622
BaeI ACNNNNGTAYC 6 cut(s) 2886, 2919, 3693, 3726, 4899, 4932
BalI TGGCCA 2 cut(s) 2234, 3878
BamHI GGATCC 2 cut(s) 1672, 1774
BanI GGYRCC 1 cut(s) 2641
BanII GRGCYC 7 cut(s) 1277, 1486, 2931, 3983, 4226, 4247, 4622
BbrPI CACGTG 1 cut(s) 4450
BbsI GAAGAC 3 cut(s) 1200, 1349, 3473
Bbv12I GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1486, 1879, 1963, 2931, 4247
BbvCI CCTCAGC 2 cut(s) 1734, 4665
BbvI GCAGC 7 cut(s) 924, 1702, 2353, 2707, 2710, 2755, 4330
BceAI ACGGC 5 cut(s) 756, 792, 972, 2782, 4048
BcgI CGANNNNNNTGC 2 cut(s) 4851, 4885
BciVI GTATCC 2 cut(s) 1975, 3634
BclI TGATCA 4 cut(s) 2437, 4993, 5077, 5137
BcnI CCSGG 2 cut(s) 129, 3170
BfaI CTAG 8 cut(s) 1422, 1706, 1938, 3192, 3900, 4023, 4941, 5272
BfmI CTRYAG 5 cut(s) 867, 2411, 2847, 4749, 5113
BfoI RGCGCY 2 cut(s) 1504, 2249
BfrI CTTAAG 1 cut(s) 2069
BfuAI ACCTGC 3 cut(s) 3376, 3745, 4555
BfuI GTATCC 2 cut(s) 1975, 3634
BglII AGATCT 4 cut(s) 60, 1594, 1852, 4943
BisI GCNGC 9 cut(s) 938, 1691, 2367, 2696, 2699, 2744, 3234, 4344, 4791
BlpI GCTNAGC 1 cut(s) 4443
BlsI GCNGC 9 cut(s) 939, 1692, 2368, 2697, 2700, 2745, 3235, 4345, 4792
BmcAI AGTACT 3 cut(s) 3064, 4507, 4702
Bme18I GGWCC 6 cut(s) 477, 545, 1336, 1394, 2997, 3147
BmgBI CACGTC 2 cut(s) 455, 1575
BmrI ACTGGG 3 cut(s) 1028, 1142, 1618
BmsI GCATC 9 cut(s) 150, 2613, 2779, 2852, 2879, 3220, 3281, 3907, 4816
BmuI ACTGGG 3 cut(s) 1028, 1142, 1618
BoxI GACNNNNGTC 1 cut(s) 2260
BpiI GAAGAC 3 cut(s) 1200, 1349, 3473
BplI GAGNNNNNCTC 4 cut(s) 1264, 1296, 1717, 1749
BpmI CTGGAG 4 cut(s) 1719, 3654, 3888, 4338
Bpu10I CCTNAGC 2 cut(s) 1734, 4665
Bpu1102I GCTNAGC 1 cut(s) 4443
Bpu14I TTCGAA 1 cut(s) 1259
BpuEI CTTGAG 7 cut(s) 469, 1107, 1972, 2943, 2952, 3161, 4473
BpuMI CCSGG 2 cut(s) 129, 3170
BsaAI YACGTR 2 cut(s) 3455, 4450
BsaBI GATNNNNATC 4 cut(s) 666, 3090, 4992, 5136
BsaWI WCCGGW 2 cut(s) 20, 1747
Bse118I RCCGGY 2 cut(s) 675, 4737
Bse3DI GCAATG 3 cut(s) 116, 3415, 4857
Bse8I GATNNNNATC 4 cut(s) 666, 3090, 4992, 5136
BseJI GATNNNNATC 4 cut(s) 666, 3090, 4992, 5136
BseMI GCAATG 3 cut(s) 116, 3415, 4857
BseRI GAGGAG 8 cut(s) 1269, 2451, 2729, 2946, 3276, 3805, 4019, 4458
BseSI GKGCMC 6 cut(s) 463, 1054, 1120, 1879, 1963, 4622
BseXI GCAGC 7 cut(s) 924, 1702, 2353, 2707, 2710, 2755, 4330
BseYI CCCAGC 4 cut(s) 2589, 4198, 4479, 5126
BsgI GTGCAG 2 cut(s) 3716, 4047
Bsh1236I CGCG 2 cut(s) 538, 1652
BshNI GGYRCC 1 cut(s) 2641
BsiHKAI GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1486, 1879, 1963, 2931, 4247
BsiSI CCGG 8 cut(s) 21, 128, 614, 676, 1748, 1836, 3170, 4738
BsiWI CGTACG 1 cut(s) 416
BslFI GGGAC 7 cut(s) 392, 1823, 2761, 3010, 3160, 3809, 4912
BsmBI CGTCTC 4 cut(s) 1377, 2862, 4071, 4593
BsmFI GGGAC 7 cut(s) 392, 1823, 2761, 3010, 3160, 3809, 4912
BsmI GAATGC 3 cut(s) 2632, 2845, 3448
Bsp119I TTCGAA 1 cut(s) 1259
Bsp120I GGGCCC 1 cut(s) 4618
Bsp1407I TGTACA 1 cut(s) 4255
Bsp1720I GCTNAGC 1 cut(s) 4443
Bsp19I CCATGG 1 cut(s) 1475
BspFNI CGCG 2 cut(s) 538, 1652
BspHI TCATGA 6 cut(s) 244, 1282, 1519, 2434, 3067, 5241
BspMAI CTGCAG 1 cut(s) 2415
BspMI ACCTGC 3 cut(s) 3376, 3745, 4555
BspQI GCTCTTC 3 cut(s) 1080, 3375, 3971
BspT104I TTCGAA 1 cut(s) 1259
BspT107I GGYRCC 1 cut(s) 2641
BspTI CTTAAG 1 cut(s) 2069
BsrDI GCAATG 3 cut(s) 116, 3415, 4857
BsrFI RCCGGY 2 cut(s) 675, 4737
BsrGI TGTACA 1 cut(s) 4255
BssAI RCCGGY 2 cut(s) 675, 4737
BssNAI GTATAC 1 cut(s) 949
BssT1I CCWWGG 5 cut(s) 680, 1475, 2063, 3504, 3860
Bst1107I GTATAC 1 cut(s) 949
Bst6I CTCTTC 8 cut(s) 700, 808, 1080, 2572, 3375, 3559, 3971, 4421
BstAFI CTTAAG 1 cut(s) 2069
BstAPI GCANNNNNTGC 2 cut(s) 458, 4078
BstAUI TGTACA 1 cut(s) 4255
BstBAI YACGTR 2 cut(s) 3455, 4450
BstBI TTCGAA 1 cut(s) 1259
BstC8I GCNNGC 9 cut(s) 320, 611, 1206, 1468, 1559, 3342, 4671, 4675, 4739
BstDSI CCRYGG 3 cut(s) 772, 808, 1475
BstENI CCTNNNNNAGG 4 cut(s) 1990, 2391, 2753, 2958
BstFNI CGCG 2 cut(s) 538, 1652
BstH2I RGCGCY 2 cut(s) 1504, 2249
BstHHI GCGC 3 cut(s) 1503, 2248, 3799
BstNSI RCATGY 4 cut(s) 613, 1444, 2431, 3344
BstPAI GACNNNNGTC 1 cut(s) 2260
BstSFI CTRYAG 5 cut(s) 867, 2411, 2847, 4749, 5113
BstSLI GKGCMC 6 cut(s) 463, 1054, 1120, 1879, 1963, 4622
BstUI CGCG 2 cut(s) 538, 1652
BstV1I GCAGC 7 cut(s) 924, 1702, 2353, 2707, 2710, 2755, 4330
BstV2I GAAGAC 3 cut(s) 1200, 1349, 3473
BstXI CCANNNNNNTGG 2 cut(s) 2242, 3632
BstZ17I GTATAC 1 cut(s) 949
BsuI GTATCC 2 cut(s) 1975, 3634
BtgI CCRYGG 3 cut(s) 772, 808, 1475
BtrI CACGTC 2 cut(s) 455, 1575
BtsI GCAGTG 2 cut(s) 850, 2408
BtsIMutI CAGTG 9 cut(s) 98, 850, 1016, 1672, 2114, 2408, 5088, 5104, 5122
BveI ACCTGC 3 cut(s) 3376, 3745, 4555
Cac8I GCNNGC 9 cut(s) 320, 611, 1206, 1468, 1559, 3342, 4671, 4675, 4739
CaiI CAGNNNCTG 2 cut(s) 3614, 4094
CciI TCATGA 6 cut(s) 244, 1282, 1519, 2434, 3067, 5241
CfoI GCGC 3 cut(s) 1503, 2248, 3799
Cfr10I RCCGGY 2 cut(s) 675, 4737
CseI GACGC 1 cut(s) 1658
CsiI ACCWGGT 1 cut(s) 3844
CspCI CAANNNNNGTGG 2 cut(s) 5146, 5181
DraI TTTAAA 1 cut(s) 3279
DraIII CACNNNGTG 2 cut(s) 458, 1907
EaeI YGGCCR 5 cut(s) 3, 805, 1623, 2232, 3876
Eam1104I CTCTTC 8 cut(s) 700, 808, 1080, 2572, 3375, 3559, 3971, 4421
EarI CTCTTC 8 cut(s) 700, 808, 1080, 2572, 3375, 3559, 3971, 4421
EciI GGCGGA 1 cut(s) 729
Ecl136II GAGCTC 4 cut(s) 1275, 1484, 2929, 4245
Eco130I CCWWGG 5 cut(s) 680, 1475, 2063, 3504, 3860
Eco24I GRGCYC 7 cut(s) 1277, 1486, 2931, 3983, 4226, 4247, 4622
Eco32I GATATC 3 cut(s) 3088, 3650, 4408
Eco47I GGWCC 6 cut(s) 477, 545, 1336, 1394, 2997, 3147
Eco47III AGCGCT 1 cut(s) 1502
Eco53kI GAGCTC 4 cut(s) 1275, 1484, 2929, 4245
Eco72I CACGTG 1 cut(s) 4450
EcoICRI GAGCTC 4 cut(s) 1275, 1484, 2929, 4245
EcoNI CCTNNNNNAGG 4 cut(s) 1990, 2391, 2753, 2958
EcoO109I RGGNCCY 7 cut(s) 545, 1394, 1796, 2997, 3147, 4295, 4618
EcoRI GAATTC 1 cut(s) 639
EcoRV GATATC 3 cut(s) 3088, 3650, 4408
EcoT14I CCWWGG 5 cut(s) 680, 1475, 2063, 3504, 3860
EcoT22I ATGCAT 1 cut(s) 2845
EcoT38I GRGCYC 7 cut(s) 1277, 1486, 2931, 3983, 4226, 4247, 4622
ErhI CCWWGG 5 cut(s) 680, 1475, 2063, 3504, 3860
Esp3I CGTCTC 4 cut(s) 1377, 2862, 4071, 4593
FalI AAGNNNNNCTT 2 cut(s) 2448, 2480
FaqI GGGAC 7 cut(s) 392, 1823, 2761, 3010, 3160, 3809, 4912
FauI CCCGC 3 cut(s) 35, 315, 2786
FbaI TGATCA 4 cut(s) 2437, 4993, 5077, 5137
FblI GTMKAC 2 cut(s) 948, 4364
Fnu4HI GCNGC 9 cut(s) 938, 1691, 2367, 2696, 2699, 2744, 3234, 4344, 4791
FriOI GRGCYC 7 cut(s) 1277, 1486, 2931, 3983, 4226, 4247, 4622
Fsp4HI GCNGC 9 cut(s) 938, 1691, 2367, 2696, 2699, 2744, 3234, 4344, 4791
FspBI CTAG 8 cut(s) 1422, 1706, 1938, 3192, 3900, 4023, 4941, 5272
GlaI GCGC 3 cut(s) 1502, 2247, 3798
GluI GCNGC 9 cut(s) 938, 1691, 2367, 2696, 2699, 2744, 3234, 4344, 4791
GsaI CCCAGC 4 cut(s) 2593, 4202, 4483, 5130
GsuI CTGGAG 4 cut(s) 1719, 3654, 3888, 4338
HaeII RGCGCY 2 cut(s) 1504, 2249
HapII CCGG 8 cut(s) 21, 128, 614, 676, 1748, 1836, 3170, 4738
HgaI GACGC 1 cut(s) 1658
HhaI GCGC 3 cut(s) 1503, 2248, 3799
Hin6I GCGC 3 cut(s) 1501, 2246, 3797
HinP1I GCGC 3 cut(s) 1501, 2246, 3797
HincII GTYRAC 5 cut(s) 748, 2536, 3667, 3838, 3855
HindII GTYRAC 5 cut(s) 748, 2536, 3667, 3838, 3855
HindIII AAGCTT 2 cut(s) 3498, 4379
HpaII CCGG 8 cut(s) 21, 128, 614, 676, 1748, 1836, 3170, 4738
HphI GGTGA 9 cut(s) 4, 37, 96, 245, 1881, 1919, 3834, 4186, 5261
Hpy99I CGWCG 5 cut(s) 312, 417, 459, 519, 2803
HpyCH4IV ACGT 5 cut(s) 454, 1574, 1911, 3454, 4449
HpySE526I ACGT 5 cut(s) 454, 1574, 1911, 3454, 4449
HspAI GCGC 3 cut(s) 1501, 2246, 3797
KflI GGGWCCC 2 cut(s) 2997, 3147
KroI GCCGGC 1 cut(s) 4737
KroNI GCCGGC 1 cut(s) 4739
Ksp22I TGATCA 4 cut(s) 2437, 4993, 5077, 5137
LguI GCTCTTC 3 cut(s) 1080, 3375, 3971
Lsp1109I GCAGC 7 cut(s) 924, 1702, 2353, 2707, 2710, 2755, 4330
LweI GCATC 9 cut(s) 150, 2613, 2779, 2852, 2879, 3220, 3281, 3907, 4816
MabI ACCWGGT 1 cut(s) 3844
MaeI CTAG 8 cut(s) 1422, 1706, 1938, 3192, 3900, 4023, 4941, 5272
MaeII ACGT 5 cut(s) 454, 1574, 1911, 3454, 4449
MlsI TGGCCA 2 cut(s) 2234, 3878
MluNI TGGCCA 2 cut(s) 2234, 3878
MmeI TCCRAC 9 cut(s) 525, 616, 815, 1011, 1783, 2167, 2497, 3697, 3710
Mox20I TGGCCA 2 cut(s) 2234, 3878
Mph1103I ATGCAT 1 cut(s) 2845
MroNI GCCGGC 1 cut(s) 4737
MroXI GAANNNNTTC 3 cut(s) 1361, 2194, 5013
MscI TGGCCA 2 cut(s) 2234, 3878
MslI CAYNNNNRTG 4 cut(s) 109, 1302, 2988, 3345
Msp20I TGGCCA 2 cut(s) 2234, 3878
MspA1I CMGCKG 5 cut(s) 1204, 2593, 4669, 4724, 5042
MspCI CTTAAG 1 cut(s) 2069
MspI CCGG 8 cut(s) 21, 128, 614, 676, 1748, 1836, 3170, 4738
Mva1269I GAATGC 3 cut(s) 2632, 2845, 3448
MvnI CGCG 2 cut(s) 538, 1652
NaeI GCCGGC 1 cut(s) 4739
NciI CCSGG 2 cut(s) 129, 3170
NcoI CCATGG 1 cut(s) 1475
NgoMIV GCCGGC 1 cut(s) 4737
NmeAIII GCCGAG 2 cut(s) 1348, 2839
NsiI ATGCAT 1 cut(s) 2845
NspI RCATGY 4 cut(s) 613, 1444, 2431, 3344
NspV TTCGAA 1 cut(s) 1259
PaeI GCATGC 2 cut(s) 613, 3344
PagI TCATGA 6 cut(s) 244, 1282, 1519, 2434, 3067, 5241
PaqCI CACCTGC 1 cut(s) 3376
PciI ACATGT 1 cut(s) 2427
PciSI GCTCTTC 3 cut(s) 1080, 3375, 3971
PctI GAATGC 3 cut(s) 2632, 2845, 3448
PdiI GCCGGC 1 cut(s) 4739
PdmI GAANNNNTTC 3 cut(s) 1361, 2194, 5013
PfeI GAWTC 9 cut(s) 88, 199, 2020, 2515, 3098, 3173, 3266, 4972, 5094
Pfl23II CGTACG 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 1576
PflMI CCANNNNNTGG 2 cut(s) 194, 1949
PfoI TCCNGGA 3 cut(s) 1948, 3168, 5049
PkrI GCNGC 9 cut(s) 939, 1692, 2368, 2697, 2700, 2745, 3235, 4345, 4792
PmaCI CACGTG 1 cut(s) 4450
PmlI CACGTG 1 cut(s) 4450
Ppu21I YACGTR 2 cut(s) 3455, 4450
PpuMI RGGWCCY 4 cut(s) 545, 1394, 2997, 3147
PscI ACATGT 1 cut(s) 2427
PshAI GACNNNNGTC 1 cut(s) 2260
PshBI ATTAAT 1 cut(s) 3927
PsiI TTATAA 1 cut(s) 1770
Psp124BI GAGCTC 4 cut(s) 1277, 1486, 2931, 4247
Psp5II RGGWCCY 4 cut(s) 545, 1394, 2997, 3147
PspCI CACGTG 1 cut(s) 4450
PspFI CCCAGC 4 cut(s) 2589, 4198, 4479, 5126
PspLI CGTACG 1 cut(s) 416
PspOMI GGGCCC 1 cut(s) 4618
PspPPI RGGWCCY 4 cut(s) 545, 1394, 2997, 3147
PsrI GAACNNNNNNTAC 2 cut(s) 1278, 1310
PstI CTGCAG 1 cut(s) 2415
PstNI CAGNNNCTG 2 cut(s) 3614, 4094
PsyI GACNNNGTC 1 cut(s) 1576
PvuII CAGCTG 4 cut(s) 2593, 4669, 4724, 5042
RseI CAYNNNNRTG 4 cut(s) 109, 1302, 2988, 3345
SacI GAGCTC 4 cut(s) 1277, 1486, 2931, 4247
SapI GCTCTTC 3 cut(s) 1080, 3375, 3971
SatI GCNGC 9 cut(s) 938, 1691, 2367, 2696, 2699, 2744, 3234, 4344, 4791
ScaI AGTACT 3 cut(s) 3064, 4507, 4702
SexAI ACCWGGT 1 cut(s) 3844
SfaNI GCATC 9 cut(s) 150, 2613, 2779, 2852, 2879, 3220, 3281, 3907, 4816
SfcI CTRYAG 5 cut(s) 867, 2411, 2847, 4749, 5113
SfuI TTCGAA 1 cut(s) 1259
SinI GGWCC 6 cut(s) 477, 545, 1336, 1394, 2997, 3147
SmiMI CAYNNNNRTG 4 cut(s) 109, 1302, 2988, 3345
SmlI CTYRAG 8 cut(s) 484, 1122, 1987, 2069, 2958, 2967, 3140, 4488
SmoI CTYRAG 8 cut(s) 484, 1122, 1987, 2069, 2958, 2967, 3140, 4488
SphI GCATGC 2 cut(s) 613, 3344
SspI AATATT 2 cut(s) 3037, 3118
SspMI CTAG 8 cut(s) 1422, 1706, 1938, 3192, 3900, 4023, 4941, 5272
SstI GAGCTC 4 cut(s) 1277, 1486, 2931, 4247
StyI CCWWGG 5 cut(s) 680, 1475, 2063, 3504, 3860
TaiI ACGT 5 cut(s) 457, 1577, 1914, 3457, 4452
TaqII GACCGA 1 cut(s) 494
TatI WGTACW 6 cut(s) 782, 3062, 3513, 4255, 4505, 4700
TauI GCSGC 2 cut(s) 3236, 4793
TfiI GAWTC 9 cut(s) 88, 199, 2020, 2515, 3098, 3173, 3266, 4972, 5094
TscAI CASTG 9 cut(s) 105, 850, 1023, 1672, 2121, 2415, 5095, 5104, 5122
TseI GCWGC 7 cut(s) 937, 1690, 2366, 2695, 2698, 2743, 4343
TspGWI ACGGA 4 cut(s) 31, 434, 660, 2844
TspRI CASTG 9 cut(s) 105, 850, 1023, 1672, 2121, 2415, 5095, 5104, 5122
Tth111I GACNNNGTC 1 cut(s) 1576
Van91I CCANNNNNTGG 2 cut(s) 194, 1949
Vha464I CTTAAG 1 cut(s) 2069
VneI GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
VpaK11BI GGWCC 6 cut(s) 477, 545, 1336, 1394, 2997, 3147
VspI ATTAAT 1 cut(s) 3927
XagI CCTNNNNNAGG 4 cut(s) 1990, 2391, 2753, 2958
XbaI TCTAGA 3 cut(s) 1705, 3899, 4940
XceI RCATGY 4 cut(s) 613, 1444, 2431, 3344
XcmI CCANNNNNNNNNTGG 4 cut(s) 471, 2462, 3713, 4356
XmiI GTMKAC 2 cut(s) 948, 4364
XmnI GAANNNNTTC 3 cut(s) 1361, 2194, 5013
XspI CTAG 8 cut(s) 1422, 1706, 1938, 3192, 3900, 4023, 4941, 5272
ZrmI AGTACT 3 cut(s) 3064, 4507, 4702
Zsp2I ATGCAT 1 cut(s) 2845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.