Rh6BG419800

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008668
Physical Location & Seq
Reverse (-)
64385939 .. 64394114
8176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG419800.1

Sequence Viewer

Length: 4977 bp
ATGGCCGACATCACCGACTCCGGTTCCCGCCACGACCTCTCCGTTACCACTCCCGCCCAGATCTCCAAAGCTGGACAAGGTTCTGAGAATCCTATTCCACTTTCACCGCAATGGCTTCTGCCAAAGGCGGGGGAGAGTAAGCCTGGAGCGTTTAGTGGGATGCAGGAAAAACCTCTCAGCCCAAATCCATCATTTGGCAATCGCTCAGACGCCTTGAAGTTGTCAGGAAATGGTGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGCTGGACATGGAAACTGGCGGTCATCATGAACGCTGGCGGGATGAAGAAAGGGACACCAATTCTGCTGTGCGCAAGGATCGGTGGAGGGATGGGGACAAAGAGCTTAATGACACTCGCAGGATGGATCGTCGGACAGAAAATGCACCAGCAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACCGACTCAAGTAACAGGGAGTCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACTCCCGTTGGGGTCCTGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCCGAGATGGCAGCATGCAGGATAAAGGCTTATCTAATGTTGGAAATCATGGAAAGGATGAGAAGGATGGGGATCACTACCGGCCTTGGAGATCAAACTCTTCCCAAATCCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCGGCAGTCAACAAATATATTCCTAGTAGGGGCCGTGGGGAAAATACACCTCCAACCTTTTCTGTTGGCCGTGGAAGGGTTAGCCCTGGTGGAAGCTTTATGAACAGTGTTCCTACAATTTCTCAGTCTGTAGGAATTTCGGACAAAGTTGAAATTGAACATGGAGAGCCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAAGACGGCTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCTGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGATTCTAAAGATGAACACATTGTTAGCTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGAGACGATGGAGGTCCTTTCAGAAAGGCCGAGGAGGCACCTAGTAGTAGGGAATTGAGCATGCCGGGAGGAGTGAATGCTCATTCTGTCACTCCATGGAGGGCTCCATCACAGGTGGAACGCTCAAATACGGTCTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGCACACCCCCTGCTATGACGTGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCTGATCCATCTTACACCAGAAATGACGCAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTGTAGATAGGGAACAGGAAATGAGAAAACCTCAGCAGCCCTCACCGGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGGCCGGATATTTTGGCATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTTAAGTAGGCCAAACCTTGGTAATGTTGGGAAGATTCATGCTGGTTTAAGCGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCGCTGATGTCTGGGAATACGAGTGTTTCTACTCATCAGAAGTTTCCTTTTTCCGAAGGTTTGCAAGGATTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGAACGACAGCGGTCAATACCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCATAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCAAGGTGGGTCAGATCCACTACAGAGTAAGATAGACATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCTTTTCCAAATCTACTATCTCAAGCTGTTGACAGTTCATCTAGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCATTATTGGATAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTAATTAGGCAACAACAGCAGCTACTTTCCCAGGTTCTTGCGGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCATCTCGGCTTCAGGCATCACAAGAAATGTTTCCACTTGGTACAAATGTGCCAGTTCCTAATATTATGCAAAATGAGCTCACTACTAAGTTTATGGGTCTGCCTCCTCAAGGCACCCAAGATATTCGTCATAATGTAAGTGATGGGGCCCCTTCTTTACCTCTACCACAGAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCATTTAATGATGTCCATCAAGATTCATTGCCAGTGTCAAATATTGCCGAGAGATCACCCTTATTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTGGTTTCTGAGACAGTTGCAGATTCTGCATCTCTAAATTCTCCTCGAAGTTTTATTTCTATGCCACCAGGAGCTCGTGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCAATTAGATTGTCAGGTTGAAGAGCAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGCGGTGACTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCAGAGAAGAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGACCTCAATTCAGGAATTTCTCCACAAAAGACGAGAGGTAACAGAGGCAGTAAATCTGAAATTGGTACTTCGGAGGCTGTGCAATCCCAACAAGGTGGCGGGGCTACTGAATCTCTTCAAGTCAAGGTTGATTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTAAATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAGTGGTAGTATCTGAAGTCCCCAATTCTGTTAATTCGCCAAGTTTATCAACTCCTTGGGCTGGCGTTGTGGCCAATTCAGACACAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAATGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTATGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTTGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAAGTTATGCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGGTTCTGCATCTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCAAACGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACCAACATCTTTAAGAGATATCCAAAAGGAGCAAGGGAAGAGGGTTTCCTCTGCTCAGCATGTGAACCAGATTACAACTCCTCAGAAATCCCAGCCAACTCAAGCCACCCGCAATGGTGCCCCTTCATGGTCACTTTCAGGATCATCTCCATCTAATCCTGCTTCTCCAATCCAGATCAATTCCCATACTTCTCAGTCAAAGTATAAAGGAGACGATGACCTATTCTGGGGCCCAATTGACCAATCAAAGCAAGAAACCAAACAGGCCGATTTTCCTCAGCTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGCGAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAAACTGCAAGTGGCAAAGGAACCGAGCGATTACTTTCATCATCACCGGCCTCTGCTCAGTCCTCTCTGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCTAGGCTAATTGGGACAAAAGGTATGCTTGTCTTTCTGCCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1658

Amino Acids

181.97

Weight (kDa)

6.57

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 589 - 633 2.6e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1770
Acc16I TGCGCA 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 3790
AccB1I GGYRCC 4 cut(s) 1417, 2650, 2975, 4550
AccB7I CCANNNNNTGG 4 cut(s) 194, 1949, 2003, 2813
AccI GTMKAC 1 cut(s) 948
AcoI YGGCCR 4 cut(s) 3, 805, 2235, 3921
AcsI RAATTY 7 cut(s) 873, 1057, 2794, 3280, 3597, 3816, 4095
AcuI CTGAAG 7 cut(s) 1392, 2858, 3453, 3468, 3870, 3885, 4749
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 2 cut(s) 458, 1907
AfaI GTAC 9 cut(s) 2627, 2690, 2905, 3453, 3649, 4137, 4302, 4320, 4747
AflII CTTAAG 1 cut(s) 1987
AflIII ACRYGT 2 cut(s) 2427, 2691
AjiI CACGTC 2 cut(s) 455, 1575
AjuI GAANNNNNNNTTGG 2 cut(s) 3969, 4001
AloI GAACNNNNNNTCC 2 cut(s) 4344, 4376
Alw21I GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1879, 1963, 2943, 3207, 3319
Alw26I GTCTC 7 cut(s) 978, 1377, 1579, 3248, 3962, 4116, 4638
Alw44I GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
AlwNI CAGNNNCTG 4 cut(s) 2093, 3176, 3269, 4139
ApaI GGGCCC 2 cut(s) 3013, 4667
ApaLI GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
ApoI RAATTY 7 cut(s) 873, 1057, 2794, 3280, 3597, 3816, 4095
AseI ATTAAT 1 cut(s) 2735
Asp700I GAANNNNTTC 4 cut(s) 1361, 2194, 2892, 3696
AspLEI GCGC 2 cut(s) 357, 4931
AsuC2I CCSGG 2 cut(s) 707, 1446
AsuII TTCGAA 1 cut(s) 1259
AvaII GGWCC 6 cut(s) 477, 545, 1394, 3074, 3147, 4407
BaeGI GKGCMC 8 cut(s) 463, 1054, 1120, 1879, 1963, 3013, 4555, 4667
BaeI ACNNNNGTAYC 2 cut(s) 2895, 2928
BalI TGGCCA 2 cut(s) 2237, 3923
BanI GGYRCC 4 cut(s) 1417, 2650, 2975, 4550
BanII GRGCYC 7 cut(s) 1277, 1486, 2943, 3013, 3319, 4028, 4667
BauI CACGAG 1 cut(s) 3318
BbsI GAAGAC 2 cut(s) 1200, 1349
Bbv12I GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1879, 1963, 2943, 3207, 3319
BbvCI CCTCAGC 2 cut(s) 1734, 4710
BceAI ACGGC 5 cut(s) 530, 756, 792, 972, 4093
BcgI CGANNNNNNTGC 2 cut(s) 4896, 4930
BciVI GTATCC 1 cut(s) 1975
BclI TGATCA 1 cut(s) 2437
BcnI CCSGG 2 cut(s) 707, 1446
BcoDI GTCTC 7 cut(s) 978, 1377, 1579, 3248, 3962, 4116, 4638
BfaI CTAG 6 cut(s) 762, 1422, 1938, 2547, 4068, 4932
BfmI CTRYAG 2 cut(s) 867, 2411
BfrI CTTAAG 1 cut(s) 1987
BfuAI ACCTGC 1 cut(s) 3790
BfuI GTATCC 1 cut(s) 1975
BglI GCCNNNNNGGC 2 cut(s) 603, 1415
BglII AGATCT 2 cut(s) 60, 1852
BlpI GCTNAGC 1 cut(s) 4488
BmcAI AGTACT 3 cut(s) 2627, 4320, 4747
Bme18I GGWCC 6 cut(s) 477, 545, 1394, 3074, 3147, 4407
BmgBI CACGTC 2 cut(s) 455, 1575
BmrI ACTGGG 3 cut(s) 1142, 1618, 4238
BmsI GCATC 8 cut(s) 150, 2861, 2888, 3220, 3281, 3952, 4226, 4861
BmuI ACTGGG 3 cut(s) 1142, 1618, 4238
BoxI GACNNNNGTC 1 cut(s) 2263
BpiI GAAGAC 2 cut(s) 1200, 1349
BplI GAGNNNNNCTC 6 cut(s) 958, 990, 1264, 1296, 1717, 1749
BpmI CTGGAG 3 cut(s) 165, 1719, 4383
Bpu10I CCTNAGC 3 cut(s) 1734, 3336, 4710
Bpu1102I GCTNAGC 1 cut(s) 4488
Bpu14I TTCGAA 1 cut(s) 1259
BpuEI CTTGAG 5 cut(s) 469, 1107, 2511, 2955, 4518
BpuMI CCSGG 2 cut(s) 707, 1446
BsaAI YACGTR 1 cut(s) 3455
BsaBI GATNNNNATC 2 cut(s) 666, 3090
BsaHI GRCGYC 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 20, 1747
Bse118I RCCGGY 3 cut(s) 675, 4782, 4834
Bse1I ACTGG 8 cut(s) 304, 1148, 1613, 1665, 2915, 3107, 3722, 4244
Bse3DI GCAATG 5 cut(s) 116, 3101, 3415, 4552, 4902
Bse8I GATNNNNATC 2 cut(s) 666, 3090
BseJI GATNNNNATC 2 cut(s) 666, 3090
BseMI GCAATG 5 cut(s) 116, 3101, 3415, 4552, 4902
BseNI ACTGG 8 cut(s) 304, 1148, 1613, 1665, 2915, 3107, 3722, 4244
BseRI GAGGAG 9 cut(s) 1269, 1427, 1464, 2451, 2738, 2958, 3276, 4064, 4503
BseSI GKGCMC 8 cut(s) 463, 1054, 1120, 1879, 1963, 3013, 4555, 4667
BseYI CCCAGC 1 cut(s) 4524
BsgI GTGCAG 3 cut(s) 3176, 3761, 4092
BshNI GGYRCC 4 cut(s) 1417, 2650, 2975, 4550
BsiHKAI GWGCWC 9 cut(s) 463, 1054, 1120, 1277, 1879, 1963, 2943, 3207, 3319
BsiSI CCGG 9 cut(s) 21, 676, 706, 740, 1445, 1748, 1836, 4783, 4835
BslFI GGGAC 6 cut(s) 350, 392, 3087, 3160, 3854, 4957
BsmAI GTCTC 7 cut(s) 978, 1377, 1579, 3248, 3962, 4116, 4638
BsmBI CGTCTC 4 cut(s) 978, 1377, 4116, 4638
BsmFI GGGAC 6 cut(s) 350, 392, 3087, 3160, 3854, 4957
BsmI GAATGC 3 cut(s) 1462, 2854, 3448
Bsp119I TTCGAA 1 cut(s) 1259
Bsp120I GGGCCC 2 cut(s) 3009, 4663
Bsp1407I TGTACA 1 cut(s) 4300
Bsp1720I GCTNAGC 1 cut(s) 4488
Bsp19I CCATGG 1 cut(s) 1475
BspHI TCATGA 6 cut(s) 244, 310, 1282, 1519, 2434, 3067
BspMI ACCTGC 1 cut(s) 3790
BspQI GCTCTTC 2 cut(s) 3375, 4016
BspT104I TTCGAA 1 cut(s) 1259
BspT107I GGYRCC 4 cut(s) 1417, 2650, 2975, 4550
BspTI CTTAAG 1 cut(s) 1987
BsrDI GCAATG 5 cut(s) 116, 3101, 3415, 4552, 4902
BsrFI RCCGGY 3 cut(s) 675, 4782, 4834
BsrGI TGTACA 1 cut(s) 4300
BsrI ACTGG 8 cut(s) 304, 1148, 1613, 1665, 2915, 3107, 3722, 4244
BssAI RCCGGY 3 cut(s) 675, 4782, 4834
BssNAI GTATAC 1 cut(s) 949
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 3318
BssT1I CCWWGG 7 cut(s) 680, 1475, 2002, 2063, 3504, 3905, 4062
Bst1107I GTATAC 1 cut(s) 949
Bst2BI CACGAG 1 cut(s) 3318
Bst4CI ACNGT 6 cut(s) 845, 1513, 2540, 2820, 3199, 3259
Bst6I CTCTTC 7 cut(s) 700, 2581, 3375, 3559, 3702, 4016, 4466
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 1987
BstAPI GCANNNNNTGC 3 cut(s) 458, 3269, 4388
BstAUI TGTACA 1 cut(s) 4300
BstBAI YACGTR 1 cut(s) 3455
BstBI TTCGAA 1 cut(s) 1259
BstC8I GCNNGC 9 cut(s) 320, 611, 1206, 1442, 3417, 3913, 4716, 4720, 4784
BstDSI CCRYGG 3 cut(s) 772, 808, 1475
BstENI CCTNNNNNAGG 2 cut(s) 1990, 2970
BstHHI GCGC 2 cut(s) 357, 4931
BstMAI GTCTC 7 cut(s) 978, 1377, 1579, 3248, 3962, 4116, 4638
BstNSI RCATGY 6 cut(s) 613, 1444, 2431, 2695, 3344, 4496
BstPAI GACNNNNGTC 1 cut(s) 2263
BstSFI CTRYAG 2 cut(s) 867, 2411
BstSLI GKGCMC 8 cut(s) 463, 1054, 1120, 1879, 1963, 3013, 4555, 4667
BstV2I GAAGAC 2 cut(s) 1200, 1349
BstX2I RGATCY 8 cut(s) 60, 1594, 1672, 1774, 1852, 2404, 2593, 2860
BstXI CCANNNNNNTGG 3 cut(s) 2245, 3199, 3677
BstYI RGATCY 8 cut(s) 60, 1594, 1672, 1774, 1852, 2404, 2593, 2860
BstZ17I GTATAC 1 cut(s) 949
BsuI GTATCC 1 cut(s) 1975
BtgI CCRYGG 3 cut(s) 772, 808, 1475
BtrI CACGTC 2 cut(s) 455, 1575
BtsI GCAGTG 1 cut(s) 3744
BtsIMutI CAGTG 6 cut(s) 850, 1672, 3114, 3744, 4272, 4410
BveI ACCTGC 1 cut(s) 3790
Cac8I GCNNGC 9 cut(s) 320, 611, 1206, 1442, 3417, 3913, 4716, 4720, 4784
CaiI CAGNNNCTG 4 cut(s) 2093, 3176, 3269, 4139
CciI TCATGA 6 cut(s) 244, 310, 1282, 1519, 2434, 3067
CfoI GCGC 2 cut(s) 357, 4931
Cfr10I RCCGGY 3 cut(s) 675, 4782, 4834
CseI GACGC 2 cut(s) 218, 1658
Csp6I GTAC 9 cut(s) 2626, 2689, 2904, 3452, 3648, 4136, 4301, 4319, 4746
CspCI CAANNNNNGTGG 2 cut(s) 3225, 3260
CviQI GTAC 9 cut(s) 2626, 2689, 2904, 3452, 3648, 4136, 4301, 4319, 4746
DraIII CACNNNGTG 2 cut(s) 458, 1907
EaeI YGGCCR 4 cut(s) 3, 805, 2235, 3921
Eam1104I CTCTTC 7 cut(s) 700, 2581, 3375, 3559, 3702, 4016, 4466
EarI CTCTTC 7 cut(s) 700, 2581, 3375, 3559, 3702, 4016, 4466
Ecl136II GAGCTC 3 cut(s) 1275, 2941, 3317
Eco130I CCWWGG 7 cut(s) 680, 1475, 2002, 2063, 3504, 3905, 4062
Eco24I GRGCYC 7 cut(s) 1277, 1486, 2943, 3013, 3319, 4028, 4667
Eco32I GATATC 1 cut(s) 4453
Eco47I GGWCC 6 cut(s) 477, 545, 1394, 3074, 3147, 4407
Eco53kI GAGCTC 3 cut(s) 1275, 2941, 3317
Eco57I CTGAAG 7 cut(s) 1392, 2858, 3453, 3468, 3870, 3885, 4749
EcoICRI GAGCTC 3 cut(s) 1275, 2941, 3317
EcoNI CCTNNNNNAGG 2 cut(s) 1990, 2970
EcoO109I RGGNCCY 8 cut(s) 545, 1394, 1796, 3009, 3010, 3147, 4340, 4663
EcoRV GATATC 1 cut(s) 4453
EcoT14I CCWWGG 7 cut(s) 680, 1475, 2002, 2063, 3504, 3905, 4062
EcoT22I ATGCAT 1 cut(s) 2854
EcoT38I GRGCYC 7 cut(s) 1277, 1486, 2943, 3013, 3319, 4028, 4667
ErhI CCWWGG 7 cut(s) 680, 1475, 2002, 2063, 3504, 3905, 4062
Esp3I CGTCTC 4 cut(s) 978, 1377, 4116, 4638
FalI AAGNNNNNCTT 2 cut(s) 4941, 4973
FaqI GGGAC 6 cut(s) 350, 392, 3087, 3160, 3854, 4957
FauI CCCGC 7 cut(s) 35, 61, 121, 315, 2795, 3674, 4550
FbaI TGATCA 1 cut(s) 2437
FblI GTMKAC 1 cut(s) 948
FriOI GRGCYC 7 cut(s) 1277, 1486, 2943, 3013, 3319, 4028, 4667
FspBI CTAG 6 cut(s) 762, 1422, 1938, 2547, 4068, 4932
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 2 cut(s) 356, 4930
GsaI CCCAGC 1 cut(s) 4528
GsuI CTGGAG 3 cut(s) 165, 1719, 4383
HapII CCGG 9 cut(s) 21, 676, 706, 740, 1445, 1748, 1836, 4783, 4835
HgaI GACGC 2 cut(s) 218, 1658
HhaI GCGC 2 cut(s) 357, 4931
Hin1I GRCGYC 1 cut(s) 210
Hin6I GCGC 2 cut(s) 355, 4929
HinP1I GCGC 2 cut(s) 355, 4929
HincII GTYRAC 2 cut(s) 748, 2536
HindII GTYRAC 2 cut(s) 748, 2536
HindIII AAGCTT 3 cut(s) 832, 1178, 4424
HpaII CCGG 9 cut(s) 21, 676, 706, 740, 1445, 1748, 1836, 4783, 4835
Hpy99I CGWCG 2 cut(s) 417, 459
HpyCH4III ACNGT 6 cut(s) 845, 1513, 2540, 2820, 3199, 3259
HpyCH4IV ACGT 3 cut(s) 454, 1574, 3454
HpySE526I ACGT 3 cut(s) 454, 1574, 3454
Hsp92I GRCGYC 1 cut(s) 210
HspAI GCGC 2 cut(s) 355, 4929
KflI GGGWCCC 1 cut(s) 3147
KroI GCCGGC 1 cut(s) 4782
KroNI GCCGGC 1 cut(s) 4784
Ksp22I TGATCA 1 cut(s) 2437
LguI GCTCTTC 2 cut(s) 3375, 4016
LweI GCATC 8 cut(s) 150, 2861, 2888, 3220, 3281, 3952, 4226, 4861
MaeI CTAG 6 cut(s) 762, 1422, 1938, 2547, 4068, 4932
MaeII ACGT 3 cut(s) 454, 1574, 3454
MfeI CAATTG 1 cut(s) 4668
MflI RGATCY 8 cut(s) 60, 1594, 1672, 1774, 1852, 2404, 2593, 2860
MlsI TGGCCA 2 cut(s) 2237, 3923
MluNI TGGCCA 2 cut(s) 2237, 3923
MlyI GAGTC 8 cut(s) 11, 476, 506, 584, 730, 2120, 4192, 4409
MmeI TCCRAC 8 cut(s) 395, 616, 815, 1783, 2167, 2497, 3742, 3755
Mox20I TGGCCA 2 cut(s) 2237, 3923
Mph1103I ATGCAT 1 cut(s) 2854
MroNI GCCGGC 1 cut(s) 4782
MroXI GAANNNNTTC 4 cut(s) 1361, 2194, 2892, 3696
MscI TGGCCA 2 cut(s) 2237, 3923
MslI CAYNNNNRTG 2 cut(s) 109, 3345
Msp20I TGGCCA 2 cut(s) 2237, 3923
MspA1I CMGCKG 4 cut(s) 1204, 2263, 3388, 4714
MspCI CTTAAG 1 cut(s) 1987
MspI CCGG 9 cut(s) 21, 676, 706, 740, 1445, 1748, 1836, 4783, 4835
MunI CAATTG 1 cut(s) 4668
Mva1269I GAATGC 3 cut(s) 1462, 2854, 3448
NaeI GCCGGC 1 cut(s) 4784
NciI CCSGG 2 cut(s) 707, 1446
NcoI CCATGG 1 cut(s) 1475
NgoMIV GCCGGC 1 cut(s) 4782
NmeAIII GCCGAG 4 cut(s) 623, 1435, 2848, 3148
NmuCI GTSAC 7 cut(s) 575, 1003, 1468, 1578, 3427, 4237, 4563
NsbI TGCGCA 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 2854
NspI RCATGY 6 cut(s) 613, 1444, 2431, 2695, 3344, 4496
NspV TTCGAA 1 cut(s) 1259
PaeI GCATGC 2 cut(s) 613, 1444
PagI TCATGA 6 cut(s) 244, 310, 1282, 1519, 2434, 3067
PciI ACATGT 2 cut(s) 2427, 2691
PciSI GCTCTTC 2 cut(s) 3375, 4016
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 3 cut(s) 1462, 2854, 3448
PdiI GCCGGC 1 cut(s) 4784
PdmI GAANNNNTTC 4 cut(s) 1361, 2194, 2892, 3696
PflFI GACNNNGTC 1 cut(s) 1576
PflMI CCANNNNNTGG 4 cut(s) 194, 1949, 2003, 2813
PfoI TCCNGGA 1 cut(s) 1948
PleI GAGTC 8 cut(s) 11, 476, 505, 584, 730, 2119, 4191, 4409
PpsI GAGTC 8 cut(s) 11, 476, 505, 584, 730, 2119, 4191, 4409
Ppu21I YACGTR 1 cut(s) 3455
PpuMI RGGWCCY 3 cut(s) 545, 1394, 3147
PscI ACATGT 2 cut(s) 2427, 2691
PshAI GACNNNNGTC 1 cut(s) 2263
PshBI ATTAAT 1 cut(s) 2735
PsiI TTATAA 1 cut(s) 1770
Psp124BI GAGCTC 3 cut(s) 1277, 2943, 3319
Psp5II RGGWCCY 3 cut(s) 545, 1394, 3147
PspFI CCCAGC 1 cut(s) 4524
PspOMI GGGCCC 2 cut(s) 3009, 4663
PspPPI RGGWCCY 3 cut(s) 545, 1394, 3147
PsrI GAACNNNNNNTAC 2 cut(s) 1278, 1310
PstNI CAGNNNCTG 4 cut(s) 2093, 3176, 3269, 4139
PsuI RGATCY 8 cut(s) 60, 1594, 1672, 1774, 1852, 2404, 2593, 2860
PsyI GACNNNGTC 1 cut(s) 1576
PvuII CAGCTG 2 cut(s) 3388, 4714
RsaI GTAC 9 cut(s) 2627, 2690, 2905, 3453, 3649, 4137, 4302, 4320, 4747
RsaNI GTAC 9 cut(s) 2626, 2689, 2904, 3452, 3648, 4136, 4301, 4319, 4746
RseI CAYNNNNRTG 2 cut(s) 109, 3345
SacI GAGCTC 3 cut(s) 1277, 2943, 3319
SapI GCTCTTC 2 cut(s) 3375, 4016
ScaI AGTACT 3 cut(s) 2627, 4320, 4747
SchI GAGTC 8 cut(s) 11, 476, 506, 584, 730, 2120, 4192, 4409
SfaNI GCATC 8 cut(s) 150, 2861, 2888, 3220, 3281, 3952, 4226, 4861
SfcI CTRYAG 2 cut(s) 867, 2411
SfuI TTCGAA 1 cut(s) 1259
SinI GGWCC 6 cut(s) 477, 545, 1394, 3074, 3147, 4407
SmiMI CAYNNNNRTG 2 cut(s) 109, 3345
SmlI CTYRAG 6 cut(s) 484, 1122, 1987, 2526, 2970, 4533
SmoI CTYRAG 6 cut(s) 484, 1122, 1987, 2526, 2970, 4533
SphI GCATGC 2 cut(s) 613, 1444
SspI AATATT 4 cut(s) 2926, 3037, 3118, 3942
SspMI CTAG 6 cut(s) 762, 1422, 1938, 2547, 4068, 4932
SstI GAGCTC 3 cut(s) 1277, 2943, 3319
StyI CCWWGG 7 cut(s) 680, 1475, 2002, 2063, 3504, 3905, 4062
TaaI ACNGT 6 cut(s) 845, 1513, 2540, 2820, 3199, 3259
TaiI ACGT 3 cut(s) 457, 1577, 3457
TaqI TCGA 4 cut(s) 976, 1259, 1310, 3289
TaqII GACCGA 1 cut(s) 494
TatI WGTACW 5 cut(s) 2625, 2688, 4300, 4318, 4745
TauI GCSGC 1 cut(s) 3236
TscAI CASTG 6 cut(s) 850, 1672, 3114, 3744, 4279, 4417
TseFI GTSAC 7 cut(s) 575, 1003, 1468, 1578, 3427, 4237, 4563
Tsp45I GTSAC 7 cut(s) 575, 1003, 1468, 1578, 3427, 4237, 4563
TspGWI ACGGA 2 cut(s) 31, 2268
TspRI CASTG 6 cut(s) 850, 1672, 3114, 3744, 4279, 4417
Tth111I GACNNNGTC 1 cut(s) 1576
Van91I CCANNNNNTGG 4 cut(s) 194, 1949, 2003, 2813
Vha464I CTTAAG 1 cut(s) 1987
VneI GTGCAC 5 cut(s) 459, 1050, 1116, 1875, 1959
VpaK11BI GGWCC 6 cut(s) 477, 545, 1394, 3074, 3147, 4407
VspI ATTAAT 1 cut(s) 2735
XagI CCTNNNNNAGG 2 cut(s) 1990, 2970
XapI RAATTY 7 cut(s) 873, 1057, 2794, 3280, 3597, 3816, 4095
XceI RCATGY 6 cut(s) 613, 1444, 2431, 2695, 3344, 4496
XcmI CCANNNNNNNNNTGG 2 cut(s) 471, 3758
XmiI GTMKAC 1 cut(s) 948
XmnI GAANNNNTTC 4 cut(s) 1361, 2194, 2892, 3696
XspI CTAG 6 cut(s) 762, 1422, 1938, 2547, 4068, 4932
ZrmI AGTACT 3 cut(s) 2627, 4320, 4747
Zsp2I ATGCAT 1 cut(s) 2854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.