RLG00000011295

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008668
Physical Location & Seq
Forward (+)
8282222 .. 8290090
7869 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011295

Sequence Viewer

Length: 5319 bp
ATGGCCGACATCACCGACTCTGGTTCCCGCCGCCACCACCTCTCTGTCACCACTCCCGCCCAGATCTCCAAAGCTGGACAAGGTTCTGAGAATCCTATTCCACTTTCACCGCAATGGCTTCTGCCAAAGGCGGGGGAGAGTAAGCCTGGAGCGTTTAGTGGGGAAAAACCTCTCAGCCCAAATCCATCATTTGGCAATCGCTCAGACGCCTTCAAGTTGTCAGGAAATGGCGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGCTGGACATGGAAACTGGTGGTCGTCATGAACGCTGGCGGGATGAAGAAAGGGACACCAATTCTGCTGTGCGCAAGGATCGGTGGAGGGATGGGGAGAAAGAGCTTAATGACACTCGCAGGATGGATCGTCGGACAGAAAATGCACCAGCAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACAGACTCAAGTAACAGGGAGTCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACTCCCGTTGGGGTCCCGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCCGAGATGGCAGCATGCAGGATAAAGGCTTATCTAATGTTGGAAATCAGGGAAAGGATGAGAAGGATGGGGATCACTACCGGCCTTGGAGATCAAACTCTTCCCAAATCCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCGGCAGTCAACAAATATATTCCTAGTAGGGGCCGTGGGGAAAATACACCTCCAACCTTTTCTGTTGGCCGTGGAAGGGTTAGCCCTGGTGGAAGCTTTATGAACAGTGTTCCTACAATTTCTCAATCAGTAGGAATTTCGGACAAAGTTGAAATTGAACATGGACAGCCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAAGACGACTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCCCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCTGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGATTCTAAAGATGAACACATTGTTAGTTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGAGACGATGGAGGTCCTTTCAGAAAGGCCGAGGAGGCACCTAGTAGTAGGGAATTGAGCATGCCGGGAGGAGTGAATGCTCATTCTGGCACTCCATGGAGGGCTCCATCACAGGTAGAACGCTCAAATACGGTCTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGCACACCCCCTGCTATGACATGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCCGATCCATCTTACACCAGAAATGACGCAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTCTAGATAGGGAACAGGAAATGAGAAAACCTCAGCAGCCCTCACCGGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGGCCGGATATTTTGGCATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAATTATGGATACCTCAAGTAGGCCAAACCTTGGTAATGTTGGGAAGATTCATACTGGTTTAAGCGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCGCTGATGTCTGGGAATACGAGTGGTTCTACACATCAGAAGTTTCCTTTTTCCGAAGGTTTGCAAGGATTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGAACGACAGCGGTCAATACCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCATAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCAAGGTGGGTCAGATCCGCTACAGAGTAAGATAGACATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCCTCTTTTCCGAATCTACTATCTCAAGCTGTTGACAGTTCATCTAGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCTGCACAGCAGATGTCGTTATTGGATAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTAATTAGACAACAACAGCAGCTCCTTTCCCAGGTTCTTGCAGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCGCCTCGGCTTCAGGCATCACAAGAAATGTTTCCACTTGGTACAAATGTGCCAATTCCTAATATGCAAAATGAGCTCACTACTAAGTTTATGGGTCTGCCTCCTCAAGGCACCCAAGATATTTGTCATAATGTAAGTGATGGGGCCCCTTCTTTACCTTTACCACAAAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCAGTTAATGATGTCCATCAAGATTCATTGCCAGTATCAAATATTGCTGAGAGATCACCCTTACTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTGGTTTCTGAGACAGTTGCACATTTTGCATCTTTAAAGTCTCCTCGAAGTTTTATTTCTATGCCACCAGGAGCTTGTGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCCATTAGATAGTCAGGTTGAAGAGCAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGCGGTGACTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCAGAGAAGAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGATCTCAATTCAGGAATTTCTCCACAAAAGACAAGAGGTAACAGAGGCAGTAAATCTGAAATTGGTACTTTGGAGGCTGTGCAATCCCAACAAGGTGGCGGGGATGCTGGATCTCTTCAAGTCAAGGTTGATTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTAAATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAGTGGTAGTATATGAAGTCCCCAATTCTGTCAATTCGCCAAGTTTATCAACTCCTTGGGCTGGCGTTGTGGCCAATTCAGACACAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAATGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTAAGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTCGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAGGTTATGCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGTTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCATTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCAAATGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACCAACATCTTTAAGAGATATCCAAAAGGAGCAAGGGAAGAGGGTTTCCTCTGCTCAGCACGTGAACCAGATTACAACTCCTCAGAAATCCCAGCCAACTCAAGCCACCCGCAATGGTGCGCCTTCATGGTCACTTTCGGGATCATCTCCATCTAAGCCTGCTTCTCCAATCCAGATCAATTCCCAAACTTCTCAGTCAAAGTATAAAGGAGACGATGACCTATTCTGGGGCCCAATTGACCAATCAAAGCAAGAAACCAAACAGGTCGATTTTCCTCAGCTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGCAAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAATCCGCAAGTGGCAAAGGAACTGAGCAATTACTTTCATCTTCACCGGCCTCTGCTCAGTCCTCTCTGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCCAGGCTAATTGGGACAAAAGATACGAGTTTCCTTGAATTTTGCTTGAAGCAATCCAGAGCTGAGGCTGAGTTGCTTCTGATAGAGAATCTTGGGTCTTACGATCCTGATCATGAGTTCATTGAAAAATTTCTTAACTACAAGGAGTTGCTACCAGCCGATGTCCTTGAAATAGCTTTTCAAAGTCGGAATGATCAAAAGGCCACTGGATTCAGTGGTGCAGGTGTCAATTCCTACGGTGCAGATGTTGGGGATGTTGATCAAGATGGTGGGTCCTCCAAAGGGGGTGGCAAGAAGAAGGGGAAGAAAGGGAAGAAGGTTAGCCCTGCCGTCTTGGGATTTAACGTTGTTAGTAACCGGATCATGATGGGTGAGATCCAGTCCGTCGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1773

Amino Acids

194.24

Weight (kDa)

6.64

Isoelectric Point (pI)

57.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 588 - 632 2.8e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1767
AarI CACCTGC 1 cut(s) 5135
Acc16I TGCGCA 1 cut(s) 353
Acc36I ACCTGC 2 cut(s) 3784, 5135
AccB1I GGYRCC 3 cut(s) 1414, 2647, 2969
AccB7I CCANNNNNTGG 4 cut(s) 191, 1946, 2000, 2810
AccI GTMKAC 1 cut(s) 945
AclI AACGTT 1 cut(s) 5268
AcoI YGGCCR 5 cut(s) 3, 802, 1620, 2232, 3915
AcsI RAATTY 8 cut(s) 870, 1054, 2791, 3591, 3810, 4089, 4961, 5051
AcuI CTGAAG 6 cut(s) 1389, 2855, 3447, 3462, 3864, 4743
AcvI CACGTG 1 cut(s) 4489
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 2 cut(s) 455, 1904
AfaI GTAC 9 cut(s) 2624, 2687, 2902, 3447, 3643, 4131, 4296, 4314, 4741
AflIII ACRYGT 2 cut(s) 2424, 2688
AjiI CACGTC 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 3963, 3995
AloI GAACNNNNNNTCC 2 cut(s) 4338, 4370
Alw21I GWGCWC 8 cut(s) 460, 1051, 1117, 1274, 1876, 1960, 2937, 3201
Alw26I GTCTC 8 cut(s) 975, 1374, 1576, 3242, 3282, 3956, 4110, 4632
Alw44I GTGCAC 5 cut(s) 456, 1047, 1113, 1872, 1956
AlwNI CAGNNNCTG 3 cut(s) 2090, 3170, 4133
ApaI GGGCCC 2 cut(s) 3007, 4661
ApaLI GTGCAC 5 cut(s) 456, 1047, 1113, 1872, 1956
ApoI RAATTY 8 cut(s) 870, 1054, 2791, 3591, 3810, 4089, 4961, 5051
AseI ATTAAT 1 cut(s) 2732
Asp700I GAANNNNTTC 4 cut(s) 1358, 2191, 2889, 5052
AspLEI GCGC 3 cut(s) 354, 4549, 4925
AsuC2I CCSGG 2 cut(s) 704, 1443
AsuII TTCGAA 1 cut(s) 1256
AvaII GGWCC 6 cut(s) 542, 1391, 3068, 3141, 4401, 5196
BaeGI GKGCMC 7 cut(s) 460, 1051, 1117, 1876, 1960, 3007, 4661
BaeI ACNNNNGTAYC 2 cut(s) 2892, 2925
BalI TGGCCA 2 cut(s) 2234, 3917
BanI GGYRCC 3 cut(s) 1414, 2647, 2969
BanII GRGCYC 6 cut(s) 1274, 1483, 2937, 3007, 4022, 4661
BbrPI CACGTG 1 cut(s) 4489
BbsI GAAGAC 2 cut(s) 1197, 1346
Bbv12I GWGCWC 8 cut(s) 460, 1051, 1117, 1274, 1876, 1960, 2937, 3201
BbvCI CCTCAGC 3 cut(s) 1731, 4704, 4986
BceAI ACGGC 5 cut(s) 527, 753, 789, 4087, 5237
BcgI CGANNNNNNTGC 2 cut(s) 4890, 4924
BciVI GTATCC 1 cut(s) 1972
BclI TGATCA 4 cut(s) 2434, 5032, 5116, 5182
BcnI CCSGG 2 cut(s) 704, 1443
BcoDI GTCTC 8 cut(s) 975, 1374, 1576, 3242, 3282, 3956, 4110, 4632
BfaI CTAG 7 cut(s) 759, 1419, 1703, 1935, 2544, 4062, 5317
BfmI CTRYAG 1 cut(s) 2408
BfuAI ACCTGC 2 cut(s) 3784, 5135
BfuI GTATCC 1 cut(s) 1972
BglI GCCNNNNNGGC 2 cut(s) 600, 1412
BglII AGATCT 2 cut(s) 63, 1849
BlpI GCTNAGC 1 cut(s) 4482
BmcAI AGTACT 3 cut(s) 2624, 4314, 4741
Bme18I GGWCC 6 cut(s) 542, 1391, 3068, 3141, 4401, 5196
BmgBI CACGTC 1 cut(s) 452
BmrI ACTGGG 3 cut(s) 1139, 1615, 4232
BmsI GCATC 7 cut(s) 2858, 2885, 3214, 3275, 3670, 3946, 4855
BmuI ACTGGG 3 cut(s) 1139, 1615, 4232
BoxI GACNNNNGTC 1 cut(s) 2260
BpiI GAAGAC 2 cut(s) 1197, 1346
BplI GAGNNNNNCTC 6 cut(s) 955, 987, 1261, 1293, 1714, 1746
BpmI CTGGAG 3 cut(s) 168, 1716, 4377
Bpu10I CCTNAGC 4 cut(s) 1731, 3330, 4704, 4986
Bpu1102I GCTNAGC 1 cut(s) 4482
Bpu14I TTCGAA 1 cut(s) 1256
BpuEI CTTGAG 7 cut(s) 466, 1104, 1969, 2508, 2949, 3155, 4512
BpuMI CCSGG 2 cut(s) 704, 1443
BsaAI YACGTR 2 cut(s) 3449, 4489
BsaBI GATNNNNATC 4 cut(s) 663, 3084, 5031, 5181
BsaHI GRCGYC 1 cut(s) 207
BsaWI WCCGGW 2 cut(s) 1744, 5280
Bse118I RCCGGY 3 cut(s) 672, 4776, 4828
Bse3DI GCAATG 5 cut(s) 119, 3095, 3409, 4546, 4896
Bse8I GATNNNNATC 4 cut(s) 663, 3084, 5031, 5181
BseJI GATNNNNATC 4 cut(s) 663, 3084, 5031, 5181
BseMI GCAATG 5 cut(s) 119, 3095, 3409, 4546, 4896
BseRI GAGGAG 9 cut(s) 1266, 1424, 1461, 2448, 2735, 2952, 3270, 4058, 4497
BseSI GKGCMC 7 cut(s) 460, 1051, 1117, 1876, 1960, 3007, 4661
BseYI CCCAGC 1 cut(s) 4518
BsgI GTGCAG 6 cut(s) 2643, 3170, 3755, 4086, 5163, 5184
BshNI GGYRCC 3 cut(s) 1414, 2647, 2969
BsiHKAI GWGCWC 8 cut(s) 460, 1051, 1117, 1274, 1876, 1960, 2937, 3201
BsiSI CCGG 9 cut(s) 673, 703, 737, 1442, 1745, 1833, 4777, 4829, 5281
BslFI GGGAC 6 cut(s) 347, 528, 3081, 3154, 3848, 4951
BsmAI GTCTC 8 cut(s) 975, 1374, 1576, 3242, 3282, 3956, 4110, 4632
BsmBI CGTCTC 4 cut(s) 975, 1374, 4110, 4632
BsmFI GGGAC 6 cut(s) 347, 528, 3081, 3154, 3848, 4951
BsmI GAATGC 3 cut(s) 1459, 2851, 3442
Bsp119I TTCGAA 1 cut(s) 1256
Bsp120I GGGCCC 2 cut(s) 3003, 4657
Bsp1407I TGTACA 1 cut(s) 4294
Bsp1720I GCTNAGC 1 cut(s) 4482
Bsp19I CCATGG 1 cut(s) 1472
BspHI TCATGA 8 cut(s) 241, 307, 1279, 1516, 2431, 3061, 5035, 5286
BspMI ACCTGC 2 cut(s) 3784, 5135
BspQI GCTCTTC 2 cut(s) 3369, 4010
BspT104I TTCGAA 1 cut(s) 1256
BspT107I GGYRCC 3 cut(s) 1414, 2647, 2969
BsrDI GCAATG 5 cut(s) 119, 3095, 3409, 4546, 4896
BsrFI RCCGGY 3 cut(s) 672, 4776, 4828
BsrGI TGTACA 1 cut(s) 4294
BssAI RCCGGY 3 cut(s) 672, 4776, 4828
BssNAI GTATAC 1 cut(s) 946
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 7 cut(s) 677, 1472, 1999, 2060, 3498, 3899, 4056
Bst1107I GTATAC 1 cut(s) 946
Bst4CI ACNGT 7 cut(s) 842, 1510, 2537, 2817, 3193, 3253, 5162
Bst6I CTCTTC 7 cut(s) 697, 2578, 3369, 3553, 3696, 4010, 4460
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 3 cut(s) 455, 3263, 4382
BstAUI TGTACA 1 cut(s) 4294
BstBAI YACGTR 2 cut(s) 3449, 4489
BstBI TTCGAA 1 cut(s) 1256
BstDSI CCRYGG 3 cut(s) 769, 805, 1472
BstENI CCTNNNNNAGG 3 cut(s) 1987, 2759, 2964
BstHHI GCGC 3 cut(s) 354, 4549, 4925
BstMAI GTCTC 8 cut(s) 975, 1374, 1576, 3242, 3282, 3956, 4110, 4632
BstNSI RCATGY 5 cut(s) 610, 1441, 2428, 2692, 3338
BstPAI GACNNNNGTC 1 cut(s) 2260
BstSFI CTRYAG 1 cut(s) 2408
BstSLI GKGCMC 7 cut(s) 460, 1051, 1117, 1876, 1960, 3007, 4661
BstV2I GAAGAC 2 cut(s) 1197, 1346
BstXI CCANNNNNNTGG 3 cut(s) 2242, 3193, 3671
BstZ17I GTATAC 1 cut(s) 946
BsuI GTATCC 1 cut(s) 1972
BtgI CCRYGG 3 cut(s) 769, 805, 1472
BtrI CACGTC 1 cut(s) 452
BtsI GCAGTG 1 cut(s) 3738
BtsIMutI CAGTG 7 cut(s) 847, 1669, 3738, 4266, 4404, 5127, 5143
BveI ACCTGC 2 cut(s) 3784, 5135
CaiI CAGNNNCTG 3 cut(s) 2090, 3170, 4133
CciI TCATGA 8 cut(s) 241, 307, 1279, 1516, 2431, 3061, 5035, 5286
CfoI GCGC 3 cut(s) 354, 4549, 4925
Cfr10I RCCGGY 3 cut(s) 672, 4776, 4828
CseI GACGC 2 cut(s) 215, 1655
Csp6I GTAC 9 cut(s) 2623, 2686, 2901, 3446, 3642, 4130, 4295, 4313, 4740
CspCI CAANNNNNGTGG 4 cut(s) 3219, 3254, 5191, 5226
CviQI GTAC 9 cut(s) 2623, 2686, 2901, 3446, 3642, 4130, 4295, 4313, 4740
DraI TTTAAA 1 cut(s) 3273
DraIII CACNNNGTG 2 cut(s) 455, 1904
EaeI YGGCCR 5 cut(s) 3, 802, 1620, 2232, 3915
Eam1104I CTCTTC 7 cut(s) 697, 2578, 3369, 3553, 3696, 4010, 4460
EarI CTCTTC 7 cut(s) 697, 2578, 3369, 3553, 3696, 4010, 4460
EciI GGCGGA 1 cut(s) 2850
Ecl136II GAGCTC 2 cut(s) 1272, 2935
Eco130I CCWWGG 7 cut(s) 677, 1472, 1999, 2060, 3498, 3899, 4056
Eco24I GRGCYC 6 cut(s) 1274, 1483, 2937, 3007, 4022, 4661
Eco32I GATATC 1 cut(s) 4447
Eco47I GGWCC 6 cut(s) 542, 1391, 3068, 3141, 4401, 5196
Eco53kI GAGCTC 2 cut(s) 1272, 2935
Eco57I CTGAAG 6 cut(s) 1389, 2855, 3447, 3462, 3864, 4743
Eco72I CACGTG 1 cut(s) 4489
EcoICRI GAGCTC 2 cut(s) 1272, 2935
EcoNI CCTNNNNNAGG 3 cut(s) 1987, 2759, 2964
EcoO109I RGGNCCY 9 cut(s) 542, 1391, 1793, 3003, 3004, 3141, 4334, 4657, 5196
EcoRV GATATC 1 cut(s) 4447
EcoT14I CCWWGG 7 cut(s) 677, 1472, 1999, 2060, 3498, 3899, 4056
EcoT22I ATGCAT 1 cut(s) 2851
EcoT38I GRGCYC 6 cut(s) 1274, 1483, 2937, 3007, 4022, 4661
ErhI CCWWGG 7 cut(s) 677, 1472, 1999, 2060, 3498, 3899, 4056
Esp3I CGTCTC 4 cut(s) 975, 1374, 4110, 4632
FaqI GGGAC 6 cut(s) 347, 528, 3081, 3154, 3848, 4951
FauI CCCGC 7 cut(s) 35, 64, 124, 312, 2792, 3668, 4544
FbaI TGATCA 4 cut(s) 2434, 5032, 5116, 5182
FblI GTMKAC 1 cut(s) 945
FriOI GRGCYC 6 cut(s) 1274, 1483, 2937, 3007, 4022, 4661
FspBI CTAG 7 cut(s) 759, 1419, 1703, 1935, 2544, 4062, 5317
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 3 cut(s) 353, 4548, 4924
GsaI CCCAGC 1 cut(s) 4522
GsuI CTGGAG 3 cut(s) 168, 1716, 4377
HapII CCGG 9 cut(s) 673, 703, 737, 1442, 1745, 1833, 4777, 4829, 5281
HgaI GACGC 2 cut(s) 215, 1655
HhaI GCGC 3 cut(s) 354, 4549, 4925
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 3 cut(s) 352, 4547, 4923
HinP1I GCGC 3 cut(s) 352, 4547, 4923
HincII GTYRAC 2 cut(s) 745, 2533
HindII GTYRAC 2 cut(s) 745, 2533
HindIII AAGCTT 3 cut(s) 829, 1175, 4418
HpaII CCGG 9 cut(s) 673, 703, 737, 1442, 1745, 1833, 4777, 4829, 5281
Hpy99I CGWCG 3 cut(s) 414, 456, 5312
HpyCH4III ACNGT 7 cut(s) 842, 1510, 2537, 2817, 3193, 3253, 5162
HpyCH4IV ACGT 4 cut(s) 451, 3448, 4488, 5268
HpySE526I ACGT 4 cut(s) 451, 3448, 4488, 5268
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 3 cut(s) 352, 4547, 4923
KflI GGGWCCC 2 cut(s) 542, 3141
KroI GCCGGC 1 cut(s) 4776
KroNI GCCGGC 1 cut(s) 4778
Ksp22I TGATCA 4 cut(s) 2434, 5032, 5116, 5182
LguI GCTCTTC 2 cut(s) 3369, 4010
LweI GCATC 7 cut(s) 2858, 2885, 3214, 3275, 3670, 3946, 4855
MaeI CTAG 7 cut(s) 759, 1419, 1703, 1935, 2544, 4062, 5317
MaeII ACGT 4 cut(s) 451, 3448, 4488, 5268
MfeI CAATTG 1 cut(s) 4662
MlsI TGGCCA 2 cut(s) 2234, 3917
MluNI TGGCCA 2 cut(s) 2234, 3917
MlyI GAGTC 8 cut(s) 11, 473, 503, 581, 727, 2117, 4186, 4403
MmeI TCCRAC 9 cut(s) 392, 613, 812, 1780, 2164, 2494, 3736, 3749, 5090
Mox20I TGGCCA 2 cut(s) 2234, 3917
Mph1103I ATGCAT 1 cut(s) 2851
MroNI GCCGGC 1 cut(s) 4776
MroXI GAANNNNTTC 4 cut(s) 1358, 2191, 2889, 5052
MscI TGGCCA 2 cut(s) 2234, 3917
MslI CAYNNNNRTG 2 cut(s) 112, 3339
Msp20I TGGCCA 2 cut(s) 2234, 3917
MspA1I CMGCKG 4 cut(s) 1201, 2260, 3382, 4708
MspI CCGG 9 cut(s) 673, 703, 737, 1442, 1745, 1833, 4777, 4829, 5281
MunI CAATTG 1 cut(s) 4662
Mva1269I GAATGC 3 cut(s) 1459, 2851, 3442
NaeI GCCGGC 1 cut(s) 4778
NciI CCSGG 2 cut(s) 704, 1443
NcoI CCATGG 1 cut(s) 1472
NgoMIV GCCGGC 1 cut(s) 4776
NmeAIII GCCGAG 3 cut(s) 620, 1432, 2845
NmuCI GTSAC 7 cut(s) 46, 572, 1000, 1575, 3421, 4231, 4557
NsbI TGCGCA 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 2851
NspI RCATGY 5 cut(s) 610, 1441, 2428, 2692, 3338
NspV TTCGAA 1 cut(s) 1256
PaeI GCATGC 2 cut(s) 610, 1441
PagI TCATGA 8 cut(s) 241, 307, 1279, 1516, 2431, 3061, 5035, 5286
PaqCI CACCTGC 1 cut(s) 5135
PciI ACATGT 2 cut(s) 2424, 2688
PciSI GCTCTTC 2 cut(s) 3369, 4010
PctI GAATGC 3 cut(s) 1459, 2851, 3442
PdiI GCCGGC 1 cut(s) 4778
PdmI GAANNNNTTC 4 cut(s) 1358, 2191, 2889, 5052
PflFI GACNNNGTC 1 cut(s) 1573
PflMI CCANNNNNTGG 4 cut(s) 191, 1946, 2000, 2810
PfoI TCCNGGA 1 cut(s) 1945
PleI GAGTC 8 cut(s) 11, 473, 502, 581, 727, 2116, 4185, 4403
PmaCI CACGTG 1 cut(s) 4489
PmlI CACGTG 1 cut(s) 4489
PpsI GAGTC 8 cut(s) 11, 473, 502, 581, 727, 2116, 4185, 4403
Ppu21I YACGTR 2 cut(s) 3449, 4489
PpuMI RGGWCCY 4 cut(s) 542, 1391, 3141, 5196
PscI ACATGT 2 cut(s) 2424, 2688
PshAI GACNNNNGTC 1 cut(s) 2260
PshBI ATTAAT 1 cut(s) 2732
PsiI TTATAA 1 cut(s) 1767
Psp124BI GAGCTC 2 cut(s) 1274, 2937
Psp1406I AACGTT 1 cut(s) 5268
Psp5II RGGWCCY 4 cut(s) 542, 1391, 3141, 5196
PspCI CACGTG 1 cut(s) 4489
PspFI CCCAGC 1 cut(s) 4518
PspOMI GGGCCC 2 cut(s) 3003, 4657
PspPPI RGGWCCY 4 cut(s) 542, 1391, 3141, 5196
PsrI GAACNNNNNNTAC 2 cut(s) 1275, 1307
PstNI CAGNNNCTG 3 cut(s) 2090, 3170, 4133
PsyI GACNNNGTC 1 cut(s) 1573
PvuII CAGCTG 2 cut(s) 3382, 4708
RsaI GTAC 9 cut(s) 2624, 2687, 2902, 3447, 3643, 4131, 4296, 4314, 4741
RsaNI GTAC 9 cut(s) 2623, 2686, 2901, 3446, 3642, 4130, 4295, 4313, 4740
RseI CAYNNNNRTG 2 cut(s) 112, 3339
SacI GAGCTC 2 cut(s) 1274, 2937
SapI GCTCTTC 2 cut(s) 3369, 4010
ScaI AGTACT 3 cut(s) 2624, 4314, 4741
SchI GAGTC 8 cut(s) 11, 473, 503, 581, 727, 2117, 4186, 4403
SfaNI GCATC 7 cut(s) 2858, 2885, 3214, 3275, 3670, 3946, 4855
SfcI CTRYAG 1 cut(s) 2408
SfuI TTCGAA 1 cut(s) 1256
SinI GGWCC 6 cut(s) 542, 1391, 3068, 3141, 4401, 5196
SmiMI CAYNNNNRTG 2 cut(s) 112, 3339
SmlI CTYRAG 7 cut(s) 481, 1119, 1984, 2523, 2964, 3134, 4527
SmoI CTYRAG 7 cut(s) 481, 1119, 1984, 2523, 2964, 3134, 4527
SphI GCATGC 2 cut(s) 610, 1441
SspI AATATT 3 cut(s) 3031, 3112, 3936
SspMI CTAG 7 cut(s) 759, 1419, 1703, 1935, 2544, 4062, 5317
SstI GAGCTC 2 cut(s) 1274, 2937
StyI CCWWGG 7 cut(s) 677, 1472, 1999, 2060, 3498, 3899, 4056
TaaI ACNGT 7 cut(s) 842, 1510, 2537, 2817, 3193, 3253, 5162
TaiI ACGT 4 cut(s) 454, 3451, 4491, 5271
TaqI TCGA 6 cut(s) 973, 1256, 1307, 3283, 4695, 5310
TatI WGTACW 5 cut(s) 2622, 2685, 4294, 4312, 4739
TauI GCSGC 2 cut(s) 33, 3230
TscAI CASTG 7 cut(s) 847, 1669, 3738, 4273, 4411, 5134, 5143
TseFI GTSAC 7 cut(s) 46, 572, 1000, 1575, 3421, 4231, 4557
Tsp45I GTSAC 7 cut(s) 46, 572, 1000, 1575, 3421, 4231, 4557
TspGWI ACGGA 2 cut(s) 2265, 5296
TspRI CASTG 7 cut(s) 847, 1669, 3738, 4273, 4411, 5134, 5143
Tth111I GACNNNGTC 1 cut(s) 1573
Van91I CCANNNNNTGG 4 cut(s) 191, 1946, 2000, 2810
VneI GTGCAC 5 cut(s) 456, 1047, 1113, 1872, 1956
VpaK11BI GGWCC 6 cut(s) 542, 1391, 3068, 3141, 4401, 5196
VspI ATTAAT 1 cut(s) 2732
XagI CCTNNNNNAGG 3 cut(s) 1987, 2759, 2964
XapI RAATTY 8 cut(s) 870, 1054, 2791, 3591, 3810, 4089, 4961, 5051
XbaI TCTAGA 1 cut(s) 1702
XceI RCATGY 5 cut(s) 610, 1441, 2428, 2692, 3338
XcmI CCANNNNNNNNNTGG 2 cut(s) 468, 3752
XmiI GTMKAC 1 cut(s) 945
XmnI GAANNNNTTC 4 cut(s) 1358, 2191, 2889, 5052
XspI CTAG 7 cut(s) 759, 1419, 1703, 1935, 2544, 4062, 5317
ZrmI AGTACT 3 cut(s) 2624, 4314, 4741
Zsp2I ATGCAT 1 cut(s) 2851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.