Rh6DG412600

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008668
Physical Location & Seq
Reverse (-)
59824308 .. 59832783
8476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG412600.1

Sequence Viewer

Length: 5265 bp
ATGTCTGCAGCTGGACAAGGTTCTGAGAATCCTATTCCACTTTCACCGCAATGGCTTCTGCCAAAGGCGGGGGAGAGTAAGCCTGGAGCGTTTAGTGGGATGCAGGAAAAACCTCTCAGCCCAAATCCATCATTTGGCAATCGTTCAGACGCCTTGAAGTTGTCAGGAAATGGTGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGCTGGACATGGAAACTGGCGGTCATCATGAACGCTGGCGGGATGAAGAAAGGGACACCAATTCTGCTGTGCGCAAGGATCGGTGGAGGGATGGGGACAAAGAGCTTAATGACACTCGCAGGATGGATCGTCGGACAGAAAATGCACCAGCAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACCGACTCAAGTAACAGGGAGTCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACTCCCGTTGGGGTCCTGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCCGAGATGGCAGCATGCAGGATAAAGGCTTATCTAATGTTGGAAATCACGGAAAGGATGAGAAGGATGGGGATCACTACCGGCCTTGGAGATCAAACTCTTCCCAAATCCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCAGCAGTCAACAAATATATTCCTAGTAGGGGCCGTGGGGAAAATACACCTCCAACCTTTTCTGTTGGCCGTGGAAGGGTTAGCCCTGGTGGAAGCTTTATGAACAGTGTTCCTACAATTTCTCAGTCTGTAGGAATTTCGGACAAAGTTGAAATTGAACATGGAGAGCCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAAGACGGCTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCTGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGATTCTAAAGATGAACACATTGTTAGCTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGAGACGATGGAGGTCCTTTCAGAAAGGCCGAGGAGGCACCTAGTAGTAGGGAATTGAGCATGCCGGGAGGAGTGAATGGTCATTCTGTCACTCCATGGAGGGCTCCATCACAGGTGGAACGCTCAAATACGGTCTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGCACACCCCCTGCTATGACGTGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCTGATCCATCTTACACCAGAAATGACGCAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTGTAGATAGGGAACAGGAAATGAGAAAACCTCAGCAGCCCTCACCGGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGGCCGGATATTTTGGCATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTTAAGTAGGCCAAACCTTGGTAATGTTGGGAAGATTCATGCTGGTTTAAGTGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCGCTGATGTCTGGGAATACGAGTGTTTCTACTCATCAGAAGTTTCCTTTTTCCGAAGGTTTGCAAGGATTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGAACGACAGCGGTCAATACCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCATAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCAAGGTGGGTCAGATCCACTACAGAGTAAGATAGACATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCTTTTCCAAATCTACTATCTCAAGCTGTTGACAGTTCATCTGGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCATTATTGGATAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTAATTAGGCAACAACAGCAGCTACTTTCCCAGGTTCTTGCGGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCATCTCGGCTTCAGGCATCACAAGAAATGTTTCCACTTGGTACAAATGTGCCAGTTCCTAATATTATGCAAAATGAGCTCACCACTAAGTTTATGGGTCTGCCTCCTCAAGGCACCCAAGATATTCGTCATAATGTAAGTGATGGGGCCCCTTCTTTACCTCTACCACAGAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCATTTAATGATGTCCATCAAGATTCATTGCCAGTGTCAAATATTGCCGAGAGATCACCCTTATTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTGGTTTCTGAGACAGTTGCAGATTCTGCATCTCTAAATTCTCCTCGAAGTTTTATTTCTATGCCACCAGGAGCTCGTGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCAATTAGATTGTCAGGTTGAAGAGCAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGCGGTGACTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCAGAGAAGAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGACCTCAATTCAGGAATTTCTCCACAAAAGACAAGAGGTAACAGAGGCAGTAAATCTGAAATTGGTACTTCGGAGGCTGTGCAATCCCAACAAGGTGGCGGGGCTACTGAATCTCTTCAAGTCAAGGTTGATTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTAAATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAGTGGTAGTATCTGAAGTCCCCAATTCTGTTAATTCGCCAAGTTTATCAACTCCTTGGGCTGGCGTTGTGGCCAATTCAGACACAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAATGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTATGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTTGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAAGTTATGCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGGTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGGGAAACAGCAAACGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACCAACATCTTTAAGAGATATCCAAAAGGAGCAAGGGAAGAGGGTTTCCTCTGCTCAGCATGTGAACCAGATTACAACTCCTCAGAAATCCCAGCCAACTCAAGCCACCCGCAATGGTGCCCCTTCATGGTCACTTTCAGGATCATCTCCATCTAAGCCTGCTTCTCCAATCCAGATCAATTCCCATACTTCTCAGTCAAAGTATAAAGGAGACGATGACCTATTCTGGGGCCCAATTGACCAATCAAAGCAAGAAACCAAACAGGCCGATTTTCCTCAGCTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGCGAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAATCCGCAAGTGGCAAAGGAACCGAGCGATTACTTTCATCTTCACCGGCCTCTGCTCAGTCCTCTCTGAAAGTAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCTAGGCTAATTGGGACAAAAGATACGAGTTTCCTTGAATTTTGCTTGAAGCAATCCAGAGCTGAGGCTGAGTTGCTTCTGATAGAGAATCTTGGGTCTTACGATCCTGATCATGAGTTCATTGAAAAATTTCTCAACTACAAGGAGTTGCTATCAGCCGATGTCCTTGAAATAGCTTTTCAAAGTCGGAATGATCAAAAGGCCACTGGATTCAGTGGTGCAGGTGTCAATTCCTACGGTGCAGATGTTGGGGATGTTGATCAAGATGGTGGGTCCTCCAAAGGGGGTGGCAAGAAGAAGGGGAAGAAAGGGAAGAAGGTTAGCCCTGCCGTCTTGGGATTTAACGTTGTTAGTAACCGGATCATGATGGGTGAGATCCAGTCAGTCGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1754

Amino Acids

192.33

Weight (kDa)

6.46

Isoelectric Point (pI)

57.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 569 - 613 2.8e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1710
AarI CACCTGC 1 cut(s) 5081
Acc16I TGCGCA 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 3730, 5081
AccB1I GGYRCC 4 cut(s) 1357, 2590, 2915, 4490
AccB7I CCANNNNNTGG 4 cut(s) 134, 1889, 1943, 2753
AccI GTMKAC 1 cut(s) 888
AclI AACGTT 1 cut(s) 5214
AcoI YGGCCR 3 cut(s) 745, 2175, 3861
AcsI RAATTY 9 cut(s) 813, 997, 2734, 3220, 3537, 3756, 4035, 4907, 4997
AcuI CTGAAG 7 cut(s) 1332, 2798, 3393, 3408, 3810, 3825, 4689
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 2 cut(s) 398, 1847
AfaI GTAC 9 cut(s) 2567, 2630, 2845, 3393, 3589, 4077, 4242, 4260, 4687
AflII CTTAAG 1 cut(s) 1927
AflIII ACRYGT 2 cut(s) 2367, 2631
AjiI CACGTC 2 cut(s) 395, 1515
AjuI GAANNNNNNNTTGG 2 cut(s) 3909, 3941
AloI GAACNNNNNNTCC 2 cut(s) 4284, 4316
Alw21I GWGCWC 9 cut(s) 403, 994, 1060, 1217, 1819, 1903, 2883, 3147, 3259
Alw26I GTCTC 7 cut(s) 918, 1317, 1519, 3188, 3902, 4056, 4578
Alw44I GTGCAC 5 cut(s) 399, 990, 1056, 1815, 1899
AlwNI CAGNNNCTG 4 cut(s) 2033, 3116, 3209, 4079
ApaI GGGCCC 2 cut(s) 2953, 4607
ApaLI GTGCAC 5 cut(s) 399, 990, 1056, 1815, 1899
ApoI RAATTY 9 cut(s) 813, 997, 2734, 3220, 3537, 3756, 4035, 4907, 4997
AseI ATTAAT 1 cut(s) 2675
Asp700I GAANNNNTTC 5 cut(s) 1301, 2134, 2832, 3636, 4998
AspLEI GCGC 2 cut(s) 297, 4871
AsuC2I CCSGG 2 cut(s) 647, 1386
AsuII TTCGAA 1 cut(s) 1199
AvaII GGWCC 7 cut(s) 417, 485, 1334, 3014, 3087, 4347, 5142
BaeGI GKGCMC 8 cut(s) 403, 994, 1060, 1819, 1903, 2953, 4495, 4607
BaeI ACNNNNGTAYC 2 cut(s) 2835, 2868
BalI TGGCCA 2 cut(s) 2177, 3863
BanI GGYRCC 4 cut(s) 1357, 2590, 2915, 4490
BanII GRGCYC 7 cut(s) 1217, 1426, 2883, 2953, 3259, 3968, 4607
BauI CACGAG 1 cut(s) 3258
BbsI GAAGAC 2 cut(s) 1140, 1289
Bbv12I GWGCWC 9 cut(s) 403, 994, 1060, 1217, 1819, 1903, 2883, 3147, 3259
BbvCI CCTCAGC 3 cut(s) 1674, 4650, 4932
BceAI ACGGC 6 cut(s) 470, 696, 732, 912, 4033, 5183
BcgI CGANNNNNNTGC 2 cut(s) 4836, 4870
BciVI GTATCC 1 cut(s) 1915
BclI TGATCA 4 cut(s) 2377, 4978, 5062, 5128
BcnI CCSGG 2 cut(s) 647, 1386
BcoDI GTCTC 7 cut(s) 918, 1317, 1519, 3188, 3902, 4056, 4578
BfaI CTAG 6 cut(s) 702, 1362, 1878, 4008, 4872, 5263
BfmI CTRYAG 3 cut(s) 6, 807, 2351
BfrI CTTAAG 1 cut(s) 1927
BfuAI ACCTGC 2 cut(s) 3730, 5081
BfuI GTATCC 1 cut(s) 1915
BglI GCCNNNNNGGC 2 cut(s) 543, 1355
BglII AGATCT 1 cut(s) 1792
BlpI GCTNAGC 1 cut(s) 4428
BmcAI AGTACT 3 cut(s) 2567, 4260, 4687
Bme18I GGWCC 7 cut(s) 417, 485, 1334, 3014, 3087, 4347, 5142
BmgBI CACGTC 2 cut(s) 395, 1515
BmrI ACTGGG 3 cut(s) 1082, 1558, 4178
BmsI GCATC 7 cut(s) 90, 2801, 2828, 3160, 3221, 3892, 4801
BmuI ACTGGG 3 cut(s) 1082, 1558, 4178
BoxI GACNNNNGTC 1 cut(s) 2203
BpiI GAAGAC 2 cut(s) 1140, 1289
BplI GAGNNNNNCTC 6 cut(s) 898, 930, 1204, 1236, 1657, 1689
BpmI CTGGAG 4 cut(s) 105, 663, 1659, 4323
Bpu10I CCTNAGC 4 cut(s) 1674, 3276, 4650, 4932
Bpu1102I GCTNAGC 1 cut(s) 4428
Bpu14I TTCGAA 1 cut(s) 1199
BpuEI CTTGAG 5 cut(s) 409, 1047, 2451, 2895, 4458
BpuMI CCSGG 2 cut(s) 647, 1386
BsaAI YACGTR 1 cut(s) 3395
BsaBI GATNNNNATC 4 cut(s) 606, 3030, 4977, 5127
BsaHI GRCGYC 1 cut(s) 150
BsaWI WCCGGW 2 cut(s) 1687, 5226
BsaXI ACNNNNNCTCC 8 cut(s) 1029, 1059, 2383, 2413, 3983, 4013, 4284, 4314
Bse118I RCCGGY 3 cut(s) 615, 4722, 4774
Bse3DI GCAATG 5 cut(s) 56, 3041, 3355, 4492, 4842
Bse8I GATNNNNATC 4 cut(s) 606, 3030, 4977, 5127
BseJI GATNNNNATC 4 cut(s) 606, 3030, 4977, 5127
BseMI GCAATG 5 cut(s) 56, 3041, 3355, 4492, 4842
BseRI GAGGAG 9 cut(s) 1209, 1367, 1404, 2391, 2678, 2898, 3216, 4004, 4443
BseSI GKGCMC 8 cut(s) 403, 994, 1060, 1819, 1903, 2953, 4495, 4607
BseYI CCCAGC 1 cut(s) 4464
BsgI GTGCAG 5 cut(s) 3116, 3701, 4032, 5109, 5130
BshNI GGYRCC 4 cut(s) 1357, 2590, 2915, 4490
BsiHKAI GWGCWC 9 cut(s) 403, 994, 1060, 1217, 1819, 1903, 2883, 3147, 3259
BsiSI CCGG 8 cut(s) 616, 646, 1385, 1688, 1776, 4723, 4775, 5227
BslFI GGGAC 6 cut(s) 290, 332, 3027, 3100, 3794, 4897
BsmAI GTCTC 7 cut(s) 918, 1317, 1519, 3188, 3902, 4056, 4578
BsmBI CGTCTC 4 cut(s) 918, 1317, 4056, 4578
BsmFI GGGAC 6 cut(s) 290, 332, 3027, 3100, 3794, 4897
BsmI GAATGC 2 cut(s) 2794, 3388
Bsp119I TTCGAA 1 cut(s) 1199
Bsp120I GGGCCC 2 cut(s) 2949, 4603
Bsp1407I TGTACA 1 cut(s) 4240
Bsp1720I GCTNAGC 1 cut(s) 4428
Bsp19I CCATGG 1 cut(s) 1415
BspHI TCATGA 8 cut(s) 184, 250, 1222, 1459, 2374, 3007, 4981, 5232
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 2 cut(s) 3730, 5081
BspQI GCTCTTC 2 cut(s) 3315, 3956
BspT104I TTCGAA 1 cut(s) 1199
BspT107I GGYRCC 4 cut(s) 1357, 2590, 2915, 4490
BspTI CTTAAG 1 cut(s) 1927
BsrDI GCAATG 5 cut(s) 56, 3041, 3355, 4492, 4842
BsrFI RCCGGY 3 cut(s) 615, 4722, 4774
BsrGI TGTACA 1 cut(s) 4240
BssAI RCCGGY 3 cut(s) 615, 4722, 4774
BssNAI GTATAC 1 cut(s) 889
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 3258
BssT1I CCWWGG 7 cut(s) 620, 1415, 1942, 2003, 3444, 3845, 4002
Bst1107I GTATAC 1 cut(s) 889
Bst2BI CACGAG 1 cut(s) 3258
Bst4CI ACNGT 7 cut(s) 785, 1453, 2480, 2760, 3139, 3199, 5108
Bst6I CTCTTC 7 cut(s) 640, 2521, 3315, 3499, 3642, 3956, 4406
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 1927
BstAPI GCANNNNNTGC 3 cut(s) 398, 3209, 4328
BstAUI TGTACA 1 cut(s) 4240
BstBAI YACGTR 1 cut(s) 3395
BstBI TTCGAA 1 cut(s) 1199
BstDSI CCRYGG 3 cut(s) 712, 748, 1415
BstENI CCTNNNNNAGG 2 cut(s) 1930, 2910
BstHHI GCGC 2 cut(s) 297, 4871
BstMAI GTCTC 7 cut(s) 918, 1317, 1519, 3188, 3902, 4056, 4578
BstNSI RCATGY 6 cut(s) 553, 1384, 2371, 2635, 3284, 4436
BstPAI GACNNNNGTC 1 cut(s) 2203
BstSFI CTRYAG 3 cut(s) 6, 807, 2351
BstSLI GKGCMC 8 cut(s) 403, 994, 1060, 1819, 1903, 2953, 4495, 4607
BstV2I GAAGAC 2 cut(s) 1140, 1289
BstX2I RGATCY 8 cut(s) 1534, 1612, 1714, 1792, 2344, 2533, 2800, 5244
BstXI CCANNNNNNTGG 3 cut(s) 2185, 3139, 3617
BstYI RGATCY 8 cut(s) 1534, 1612, 1714, 1792, 2344, 2533, 2800, 5244
BstZ17I GTATAC 1 cut(s) 889
BsuI GTATCC 1 cut(s) 1915
BtgI CCRYGG 3 cut(s) 712, 748, 1415
BtrI CACGTC 2 cut(s) 395, 1515
BtsI GCAGTG 1 cut(s) 3684
BtsIMutI CAGTG 8 cut(s) 790, 1612, 3054, 3684, 4212, 4350, 5073, 5089
BveI ACCTGC 2 cut(s) 3730, 5081
CaiI CAGNNNCTG 4 cut(s) 2033, 3116, 3209, 4079
CciI TCATGA 8 cut(s) 184, 250, 1222, 1459, 2374, 3007, 4981, 5232
CfoI GCGC 2 cut(s) 297, 4871
Cfr10I RCCGGY 3 cut(s) 615, 4722, 4774
CseI GACGC 2 cut(s) 158, 1598
Csp6I GTAC 9 cut(s) 2566, 2629, 2844, 3392, 3588, 4076, 4241, 4259, 4686
CspCI CAANNNNNGTGG 4 cut(s) 3165, 3200, 5137, 5172
CviQI GTAC 9 cut(s) 2566, 2629, 2844, 3392, 3588, 4076, 4241, 4259, 4686
DraIII CACNNNGTG 2 cut(s) 398, 1847
EaeI YGGCCR 3 cut(s) 745, 2175, 3861
Eam1104I CTCTTC 7 cut(s) 640, 2521, 3315, 3499, 3642, 3956, 4406
EarI CTCTTC 7 cut(s) 640, 2521, 3315, 3499, 3642, 3956, 4406
Ecl136II GAGCTC 3 cut(s) 1215, 2881, 3257
Eco130I CCWWGG 7 cut(s) 620, 1415, 1942, 2003, 3444, 3845, 4002
Eco24I GRGCYC 7 cut(s) 1217, 1426, 2883, 2953, 3259, 3968, 4607
Eco32I GATATC 1 cut(s) 4393
Eco47I GGWCC 7 cut(s) 417, 485, 1334, 3014, 3087, 4347, 5142
Eco53kI GAGCTC 3 cut(s) 1215, 2881, 3257
Eco57I CTGAAG 7 cut(s) 1332, 2798, 3393, 3408, 3810, 3825, 4689
EcoICRI GAGCTC 3 cut(s) 1215, 2881, 3257
EcoNI CCTNNNNNAGG 2 cut(s) 1930, 2910
EcoO109I RGGNCCY 9 cut(s) 485, 1334, 1736, 2949, 2950, 3087, 4280, 4603, 5142
EcoRV GATATC 1 cut(s) 4393
EcoT14I CCWWGG 7 cut(s) 620, 1415, 1942, 2003, 3444, 3845, 4002
EcoT22I ATGCAT 1 cut(s) 2794
EcoT38I GRGCYC 7 cut(s) 1217, 1426, 2883, 2953, 3259, 3968, 4607
ErhI CCWWGG 7 cut(s) 620, 1415, 1942, 2003, 3444, 3845, 4002
Esp3I CGTCTC 4 cut(s) 918, 1317, 4056, 4578
FaqI GGGAC 6 cut(s) 290, 332, 3027, 3100, 3794, 4897
FauI CCCGC 5 cut(s) 61, 255, 2735, 3614, 4490
FbaI TGATCA 4 cut(s) 2377, 4978, 5062, 5128
FblI GTMKAC 1 cut(s) 888
FriOI GRGCYC 7 cut(s) 1217, 1426, 2883, 2953, 3259, 3968, 4607
FspBI CTAG 6 cut(s) 702, 1362, 1878, 4008, 4872, 5263
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 2 cut(s) 296, 4870
GsaI CCCAGC 1 cut(s) 4468
GsuI CTGGAG 4 cut(s) 105, 663, 1659, 4323
HapII CCGG 8 cut(s) 616, 646, 1385, 1688, 1776, 4723, 4775, 5227
HgaI GACGC 2 cut(s) 158, 1598
HhaI GCGC 2 cut(s) 297, 4871
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 2 cut(s) 295, 4869
HinP1I GCGC 2 cut(s) 295, 4869
HincII GTYRAC 2 cut(s) 688, 2476
HindII GTYRAC 2 cut(s) 688, 2476
HindIII AAGCTT 3 cut(s) 772, 1118, 4364
HpaII CCGG 8 cut(s) 616, 646, 1385, 1688, 1776, 4723, 4775, 5227
Hpy99I CGWCG 2 cut(s) 357, 399
HpyCH4III ACNGT 7 cut(s) 785, 1453, 2480, 2760, 3139, 3199, 5108
HpyCH4IV ACGT 4 cut(s) 394, 1514, 3394, 5214
HpySE526I ACGT 4 cut(s) 394, 1514, 3394, 5214
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 2 cut(s) 295, 4869
KflI GGGWCCC 1 cut(s) 3087
KroI GCCGGC 1 cut(s) 4722
KroNI GCCGGC 1 cut(s) 4724
Ksp22I TGATCA 4 cut(s) 2377, 4978, 5062, 5128
LguI GCTCTTC 2 cut(s) 3315, 3956
LweI GCATC 7 cut(s) 90, 2801, 2828, 3160, 3221, 3892, 4801
MaeI CTAG 6 cut(s) 702, 1362, 1878, 4008, 4872, 5263
MaeII ACGT 4 cut(s) 394, 1514, 3394, 5214
MfeI CAATTG 1 cut(s) 4608
MflI RGATCY 8 cut(s) 1534, 1612, 1714, 1792, 2344, 2533, 2800, 5244
MlsI TGGCCA 2 cut(s) 2177, 3863
MluNI TGGCCA 2 cut(s) 2177, 3863
MlyI GAGTC 7 cut(s) 416, 446, 524, 670, 2060, 4132, 4349
MmeI TCCRAC 9 cut(s) 335, 556, 755, 1723, 2107, 2437, 3682, 3695, 5036
Mox20I TGGCCA 2 cut(s) 2177, 3863
Mph1103I ATGCAT 1 cut(s) 2794
MroNI GCCGGC 1 cut(s) 4722
MroXI GAANNNNTTC 5 cut(s) 1301, 2134, 2832, 3636, 4998
MscI TGGCCA 2 cut(s) 2177, 3863
MslI CAYNNNNRTG 2 cut(s) 49, 3285
Msp20I TGGCCA 2 cut(s) 2177, 3863
MspA1I CMGCKG 5 cut(s) 11, 1144, 2203, 3328, 4654
MspCI CTTAAG 1 cut(s) 1927
MspI CCGG 8 cut(s) 616, 646, 1385, 1688, 1776, 4723, 4775, 5227
MunI CAATTG 1 cut(s) 4608
Mva1269I GAATGC 2 cut(s) 2794, 3388
NaeI GCCGGC 1 cut(s) 4724
NciI CCSGG 2 cut(s) 647, 1386
NcoI CCATGG 1 cut(s) 1415
NgoMIV GCCGGC 1 cut(s) 4722
NmeAIII GCCGAG 4 cut(s) 563, 1375, 2788, 3088
NmuCI GTSAC 7 cut(s) 515, 943, 1408, 1518, 3367, 4177, 4503
NsbI TGCGCA 1 cut(s) 296
NsiI ATGCAT 1 cut(s) 2794
NspI RCATGY 6 cut(s) 553, 1384, 2371, 2635, 3284, 4436
NspV TTCGAA 1 cut(s) 1199
PaeI GCATGC 2 cut(s) 553, 1384
PagI TCATGA 8 cut(s) 184, 250, 1222, 1459, 2374, 3007, 4981, 5232
PaqCI CACCTGC 1 cut(s) 5081
PciI ACATGT 2 cut(s) 2367, 2631
PciSI GCTCTTC 2 cut(s) 3315, 3956
PctI GAATGC 2 cut(s) 2794, 3388
PdiI GCCGGC 1 cut(s) 4724
PdmI GAANNNNTTC 5 cut(s) 1301, 2134, 2832, 3636, 4998
PflFI GACNNNGTC 1 cut(s) 1516
PflMI CCANNNNNTGG 4 cut(s) 134, 1889, 1943, 2753
PfoI TCCNGGA 1 cut(s) 1888
PleI GAGTC 7 cut(s) 416, 445, 524, 670, 2059, 4131, 4349
PpsI GAGTC 7 cut(s) 416, 445, 524, 670, 2059, 4131, 4349
Ppu21I YACGTR 1 cut(s) 3395
PpuMI RGGWCCY 4 cut(s) 485, 1334, 3087, 5142
PscI ACATGT 2 cut(s) 2367, 2631
PshAI GACNNNNGTC 1 cut(s) 2203
PshBI ATTAAT 1 cut(s) 2675
PsiI TTATAA 1 cut(s) 1710
Psp124BI GAGCTC 3 cut(s) 1217, 2883, 3259
Psp1406I AACGTT 1 cut(s) 5214
Psp5II RGGWCCY 4 cut(s) 485, 1334, 3087, 5142
PspFI CCCAGC 1 cut(s) 4464
PspOMI GGGCCC 2 cut(s) 2949, 4603
PspPPI RGGWCCY 4 cut(s) 485, 1334, 3087, 5142
PsrI GAACNNNNNNTAC 2 cut(s) 1218, 1250
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 4 cut(s) 2033, 3116, 3209, 4079
PsuI RGATCY 8 cut(s) 1534, 1612, 1714, 1792, 2344, 2533, 2800, 5244
PsyI GACNNNGTC 1 cut(s) 1516
PvuII CAGCTG 3 cut(s) 11, 3328, 4654
RsaI GTAC 9 cut(s) 2567, 2630, 2845, 3393, 3589, 4077, 4242, 4260, 4687
RsaNI GTAC 9 cut(s) 2566, 2629, 2844, 3392, 3588, 4076, 4241, 4259, 4686
RseI CAYNNNNRTG 2 cut(s) 49, 3285
SacI GAGCTC 3 cut(s) 1217, 2883, 3259
SapI GCTCTTC 2 cut(s) 3315, 3956
ScaI AGTACT 3 cut(s) 2567, 4260, 4687
SchI GAGTC 7 cut(s) 416, 446, 524, 670, 2060, 4132, 4349
SfaNI GCATC 7 cut(s) 90, 2801, 2828, 3160, 3221, 3892, 4801
SfcI CTRYAG 3 cut(s) 6, 807, 2351
SfuI TTCGAA 1 cut(s) 1199
SinI GGWCC 7 cut(s) 417, 485, 1334, 3014, 3087, 4347, 5142
SmiMI CAYNNNNRTG 2 cut(s) 49, 3285
SmlI CTYRAG 6 cut(s) 424, 1062, 1927, 2466, 2910, 4473
SmoI CTYRAG 6 cut(s) 424, 1062, 1927, 2466, 2910, 4473
SphI GCATGC 2 cut(s) 553, 1384
SspI AATATT 4 cut(s) 2866, 2977, 3058, 3882
SspMI CTAG 6 cut(s) 702, 1362, 1878, 4008, 4872, 5263
SstI GAGCTC 3 cut(s) 1217, 2883, 3259
StyI CCWWGG 7 cut(s) 620, 1415, 1942, 2003, 3444, 3845, 4002
TaaI ACNGT 7 cut(s) 785, 1453, 2480, 2760, 3139, 3199, 5108
TaiI ACGT 4 cut(s) 397, 1517, 3397, 5217
TaqI TCGA 5 cut(s) 916, 1199, 1250, 3229, 5256
TaqII GACCGA 1 cut(s) 434
TatI WGTACW 5 cut(s) 2565, 2628, 4240, 4258, 4685
TauI GCSGC 1 cut(s) 3176
TscAI CASTG 8 cut(s) 790, 1612, 3054, 3684, 4219, 4357, 5080, 5089
TseFI GTSAC 7 cut(s) 515, 943, 1408, 1518, 3367, 4177, 4503
Tsp45I GTSAC 7 cut(s) 515, 943, 1408, 1518, 3367, 4177, 4503
TspGWI ACGGA 2 cut(s) 600, 2208
TspRI CASTG 8 cut(s) 790, 1612, 3054, 3684, 4219, 4357, 5080, 5089
Tth111I GACNNNGTC 1 cut(s) 1516
Van91I CCANNNNNTGG 4 cut(s) 134, 1889, 1943, 2753
Vha464I CTTAAG 1 cut(s) 1927
VneI GTGCAC 5 cut(s) 399, 990, 1056, 1815, 1899
VpaK11BI GGWCC 7 cut(s) 417, 485, 1334, 3014, 3087, 4347, 5142
VspI ATTAAT 1 cut(s) 2675
XagI CCTNNNNNAGG 2 cut(s) 1930, 2910
XapI RAATTY 9 cut(s) 813, 997, 2734, 3220, 3537, 3756, 4035, 4907, 4997
XceI RCATGY 6 cut(s) 553, 1384, 2371, 2635, 3284, 4436
XcmI CCANNNNNNNNNTGG 3 cut(s) 411, 2893, 3698
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 5 cut(s) 1301, 2134, 2832, 3636, 4998
XspI CTAG 6 cut(s) 702, 1362, 1878, 4008, 4872, 5263
ZrmI AGTACT 3 cut(s) 2567, 4260, 4687
Zsp2I ATGCAT 1 cut(s) 2794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.