RchiOBHm_Chr6g0300861

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008668
Physical Location & Seq
Reverse (-)
61550697 .. 61559165
8469 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27020

Sequence Viewer

Length: 5583 bp
ATGAAAATTACTAACTCGTTGAACAGAAAAGTAAGGGTGAGACAACAAATCGATACACCTCAAACGTGCGCTGTGTGGTTAATTGCGTCTCTTTTTTTTTTTTTTTTTTTTTGGAAGAGCAATTTTTTGTTGTTGTTGCCTCAGAACTTATATTGCCACCCTTCTCTCGCGTGTGTCTTACCTCACTCCTTCAGCGCAAAAAGGGTTTCTTTGGCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCAACTTAATCGATCATCCATGGCCGACATCACCGACTCCGGTTCCCGCCACCACCTCTCCGTCACCACTCCCGCCCAGATCTCCAAAGCTGGACAAGGTTCTGAGAATCCTATTCCGCTTTCACCGCAATGGCTTCTGCCAAAGGCGGGGGAGAGTAAGCCTGGAGCTTTTAATGGGATGCAGGAAAAACCTCTCAGCCCAAATCCATCATTTGGCAATCGCTCAGATGCCTTGAAGTTGTCAGGAAATGGTGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGCTGGACATGGAAACTGGTGGTCGTCATGAACGCTGGCGGGATGAAGAAAGGGACACCAATTCTGCTGTGCGCAAGGATCGGTGGAGGGATGGGGACAAAGAGCTTAATGACACTCGCAGGATGGATCGTCGGACAGAAAATGCACCAGCAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACCGACTCAAGTAACAGGGAGTCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACTCCCGTTGGGGTCCTGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCCGAGATGGCAGCATGCAGGATAAAGGCTTATCTAATGTTGGAAATCACGGAAAGGATGAGAAGGATGGGGATCACTATCGGCCTTGGAGATCAAACTCTTCCCAAATCCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCGGCAGTCAACAAATATATTCCTAGTAGGGGCCGTGGGGAAAATACACCTCCAACCTTTTCTGTTGGCCGTGGAAGGGTTAGCCCTGGTGGAAGCTTTATGAACAGTGTTTCTACAATTTCTCAATCTGTAGGAATTTTGGACAAAGTTGAAATTGAACATGGAGAGCCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAGGACGGCTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCAGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGATTCTAAAGATGAACACATTGTTAGTTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGGGACGATGGAGGTCCTTTCAGAAAGGCCGAGGAGGCACCTAGTAGTAGGGAATTGAGCATGCCGGGAGGAGTGAATGCTCATTCTGGCACTCCATGGAGGGCTCCATCACAGGTAGAACGCTCAAATACGGTCTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGGGCACCCCCTGCTATGACGTGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCCGAATCTTACACCAGAAATGACACAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTCTAGATAGGGAACAGGAAGTAAGAAAACCTCAGCAGCCCTCACCGGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGGCCGGATATTTTGGCATAGATCTGCAAGTTCGTGTGGCAAATGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTCAAGTAGGCCAAACTTTGGTAATGTTGGGAAGAATCATTCTGGTTTAAGCGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCACTGATGTCTGGGAATATGAGTGGTTCTACTCATCAGAAGTTTCCTTTTTCCGAAGGTTTGCAAGGATTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGATCGACAGCGGTCAATACCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCTTAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCAAGGTGGGTCAGATCCACTACAGAGTAAGATAGATATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCTTTTCCGAATCTACTCGCTCAAGCTGTTGACAGTTCATCTAGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCATTATTGGATAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTACTTAGGCAACAACAGCAGCTCCTTTCCCAGGTTCTTGCGGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCGTCTCGGCTTCAGGCATCACAAGAAATGTTTCCAGTTGGTACAAATGTGCCGGTTCCTAATATGCAAAATGAGCTCACTACTAAGTTTATGGGTCTACCTCCTCAAGGCACCCAAGATATTCGTCATAATGTAAGTGATGGGGCCCCTTCTTTACCTCTACCACAAAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCGTTTAATGATGTCCATCAAGATTCATTGCCAGTGTCAAATATTGCCGAGAGATCACCCTTACTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTTGTTTCTGAGACAGTTGCAGATTCTGCATCTTTAAAGTCTTCTCGAAATTTTATTCCTATGCCACCAGGAGCTTGTGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCAATTAGATAGTCAGCTTGAAGAGCAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGCGGTGACTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCGGAGAAAAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGACCTCAATTCAGGAATTTCTCCTCAAAAGACAAGAGGTGACAGAGGCAGTAAATCTGAAATTGGTACTTCAGAGGCTGTGCAATCCCAACAAGGTGGCGGGGATGCTGAATCTCTTCAAGTCAAGGTTGACTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTACATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAATGGTAGTATCTGAAGTCCCCAATTCTGTCAATTCGCAAAGTTTATCAACTCCTTGGGCTGGCATTGTGGCCAATTCAGACGCAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAAAGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTAAGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTTGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAAGTTATTCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGGTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAGGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCAAACGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACGAACATCTTTAAGAGATATCCAAAAGGAGCAAGGCAAGAGGGTTTCCTCTGCTCAGCACGTGAACCAGATTACAACTCCTCAGAAATCCCAGCCAACTCAAGCCACCCGCAATGGTGCCCCTTCATGGTCACTTTCAGGATCATCTCCATCTAAGCCTGCTTCTCCAATCCAGATCAATTCCCATACTTCTCAGTCAAATTATAAAGGAGACGATGACCTATTCTGGGGCCCGATTGACCAATCAAAGCAAGAAACCAAACAGGCCGATTTTCCTCAGTTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGCGAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAATCCGCAAGTGGCAAAGGAACTGAGCGATTACTTTCATCTTCACTGGCCTCTGCTCAGTCCTCTCTGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCTAGGCTAATTGGGACAAAAGATACGAGTTTCCTTGAATTTTGCTTGAAGCAATCTAGAGCTGAGGCTGAGTTGCTTCTGATAGAGAATCTTGGGTCTTACGATCCTGAACATGAGTTCATTGAAAAATTTCTCAACTACAAGGAGTTGCTACCAGCGGATGTCCTTGAAATAGCTTTTCAAAGTCGGAATGATCAAAAGGCCGCCACTGGATTCAGTGGTGCAGGTGTCAATTCCTACGGTGCAGATGTTGGGGATGTTGATCAAGATGGTGGGTCCTCCAAAGGGGGTGGCAAGAAGAAGGGGAAGAAAGGGAAGAAGGTTAGCCCTGCCGTCTTGGGATTTAACGTTGTTAGTAACCGGATCATGATGGGTGAGATCCAGTCAGTCGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1860

Amino Acids

204.45

Weight (kDa)

7.28

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 675 - 719 2.9e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2028, 4893
AarI CACCTGC 1 cut(s) 5399
Acc16I TGCGCA 1 cut(s) 617
Acc36I ACCTGC 2 cut(s) 4045, 5399
AccB1I GGYRCC 5 cut(s) 1678, 1819, 2908, 3230, 4805
AccB7I CCANNNNNTGG 4 cut(s) 455, 2207, 2261, 3071
AccI GTMKAC 2 cut(s) 1209, 3217
AccII CGCG 1 cut(s) 170
AclI AACGTT 1 cut(s) 5532
AcoI YGGCCR 5 cut(s) 264, 1066, 1884, 2493, 4176
AcsI RAATTY 9 cut(s) 1134, 1318, 3052, 3547, 3852, 4071, 4350, 5222, 5312
AcuI CTGAAG 8 cut(s) 175, 1653, 3116, 3708, 3723, 3891, 4140, 5004
AcvI CACGTG 1 cut(s) 4750
AdeI CACNNNGTG 2 cut(s) 719, 2165
AflIII ACRYGT 2 cut(s) 2949, 4012
AjiI CACGTC 2 cut(s) 716, 1836
AjuI GAANNNNNNNTTGG 2 cut(s) 4224, 4256
AloI GAACNNNNNNTCC 2 cut(s) 4599, 4631
Alw21I GWGCWC 7 cut(s) 724, 1315, 1381, 1538, 2221, 3198, 3462
Alw26I GTCTC 9 cut(s) 34, 93, 1239, 1840, 3129, 3503, 4217, 4371, 4893
Alw44I GTGCAC 4 cut(s) 720, 1311, 1377, 2217
AlwNI CAGNNNCTG 5 cut(s) 2351, 3431, 3524, 3914, 4394
ApaI GGGCCC 2 cut(s) 3268, 4922
ApaLI GTGCAC 4 cut(s) 720, 1311, 1377, 2217
ApoI RAATTY 9 cut(s) 1134, 1318, 3052, 3547, 3852, 4071, 4350, 5222, 5312
Asp700I GAANNNNTTC 5 cut(s) 1622, 2452, 3150, 3951, 5313
AspLEI GCGC 4 cut(s) 71, 197, 618, 5186
AsuC2I CCSGG 2 cut(s) 968, 1707
AsuII TTCGAA 1 cut(s) 1520
AvaII GGWCC 7 cut(s) 738, 806, 1655, 3329, 3402, 4662, 5460
BaeGI GKGCMC 8 cut(s) 724, 1315, 1381, 1822, 2221, 3268, 4810, 4922
BaeI ACNNNNGTAYC 2 cut(s) 3153, 3186
BalI TGGCCA 2 cut(s) 2495, 4178
BanI GGYRCC 5 cut(s) 1678, 1819, 2908, 3230, 4805
BanII GRGCYC 6 cut(s) 1538, 1747, 3198, 3268, 4283, 4922
BbrPI CACGTG 1 cut(s) 4750
BbsI GAAGAC 3 cut(s) 1461, 1610, 3531
Bbv12I GWGCWC 7 cut(s) 724, 1315, 1381, 1538, 2221, 3198, 3462
BbvCI CCTCAGC 2 cut(s) 1992, 5247
BceAI ACGGC 6 cut(s) 791, 1017, 1053, 1233, 4348, 5501
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 5151, 5185
BciVI GTATCC 1 cut(s) 2233
BclI TGATCA 3 cut(s) 2695, 5377, 5446
BcnI CCSGG 2 cut(s) 968, 1707
BcoDI GTCTC 9 cut(s) 34, 93, 1239, 1840, 3129, 3503, 4217, 4371, 4893
BfaI CTAG 9 cut(s) 1023, 1683, 1964, 2196, 2805, 4323, 5187, 5241, 5581
BfmI CTRYAG 2 cut(s) 1128, 2669
BfuAI ACCTGC 2 cut(s) 4045, 5399
BfuI GTATCC 1 cut(s) 2233
BglI GCCNNNNNGGC 2 cut(s) 864, 1676
BglII AGATCT 2 cut(s) 321, 2110
BlpI GCTNAGC 1 cut(s) 4743
BmcAI AGTACT 3 cut(s) 2885, 4575, 5002
Bme18I GGWCC 7 cut(s) 738, 806, 1655, 3329, 3402, 4662, 5460
BmgBI CACGTC 2 cut(s) 716, 1836
BmrI ACTGGG 3 cut(s) 1403, 1879, 4493
BmsI GCATC 9 cut(s) 411, 460, 3119, 3146, 3475, 3536, 3931, 4207, 5116
BmuI ACTGGG 3 cut(s) 1403, 1879, 4493
BoxI GACNNNNGTC 1 cut(s) 2521
BpiI GAAGAC 3 cut(s) 1461, 1610, 3531
BplI GAGNNNNNCTC 4 cut(s) 1219, 1251, 1525, 1557
BpmI CTGGAG 3 cut(s) 426, 1977, 4638
Bpu10I CCTNAGC 3 cut(s) 1992, 3591, 5247
Bpu1102I GCTNAGC 1 cut(s) 4743
Bpu14I TTCGAA 1 cut(s) 1520
BpuEI CTTGAG 7 cut(s) 730, 1368, 2230, 2769, 3210, 3416, 4773
BpuMI CCSGG 2 cut(s) 968, 1707
Bsa29I ATCGAT 2 cut(s) 51, 253
BsaAI YACGTR 2 cut(s) 3710, 4750
BsaBI GATNNNNATC 5 cut(s) 927, 933, 2688, 3345, 5445
BsaWI WCCGGW 3 cut(s) 281, 2005, 5544
Bse118I RCCGGY 2 cut(s) 3172, 5037
Bse3DI GCAATG 5 cut(s) 377, 3356, 3670, 4807, 5157
Bse8I GATNNNNATC 5 cut(s) 927, 933, 2688, 3345, 5445
BseCI ATCGAT 2 cut(s) 51, 253
BseJI GATNNNNATC 5 cut(s) 927, 933, 2688, 3345, 5445
BseMI GCAATG 5 cut(s) 377, 3356, 3670, 4807, 5157
BseRI GAGGAG 9 cut(s) 1530, 1688, 1725, 2709, 2996, 3213, 3849, 4319, 4758
BseSI GKGCMC 8 cut(s) 724, 1315, 1381, 1822, 2221, 3268, 4810, 4922
BseYI CCCAGC 1 cut(s) 4779
BsgI GTGCAG 5 cut(s) 3431, 4016, 4347, 5427, 5448
Bsh1236I CGCG 1 cut(s) 170
BshNI GGYRCC 5 cut(s) 1678, 1819, 2908, 3230, 4805
BshVI ATCGAT 2 cut(s) 51, 253
BsiHKAI GWGCWC 7 cut(s) 724, 1315, 1381, 1538, 2221, 3198, 3462
BsiSI CCGG 9 cut(s) 282, 967, 1001, 1706, 2006, 2094, 3173, 5038, 5545
BslFI GGGAC 7 cut(s) 611, 653, 1658, 3342, 3415, 4109, 5212
BsmAI GTCTC 9 cut(s) 34, 93, 1239, 1840, 3129, 3503, 4217, 4371, 4893
BsmBI CGTCTC 5 cut(s) 93, 1239, 3129, 4371, 4893
BsmFI GGGAC 7 cut(s) 611, 653, 1658, 3342, 3415, 4109, 5212
BsmI GAATGC 3 cut(s) 1723, 3112, 3703
Bsp119I TTCGAA 1 cut(s) 1520
Bsp120I GGGCCC 2 cut(s) 3264, 4918
Bsp1407I TGTACA 1 cut(s) 4555
Bsp1720I GCTNAGC 1 cut(s) 4743
Bsp19I CCATGG 2 cut(s) 261, 1736
BspDI ATCGAT 2 cut(s) 51, 253
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 7 cut(s) 505, 571, 1543, 1780, 2692, 3322, 5550
BspMI ACCTGC 2 cut(s) 4045, 5399
BspQI GCTCTTC 3 cut(s) 110, 3630, 4271
BspT104I TTCGAA 1 cut(s) 1520
BspT107I GGYRCC 5 cut(s) 1678, 1819, 2908, 3230, 4805
BsrDI GCAATG 5 cut(s) 377, 3356, 3670, 4807, 5157
BsrFI RCCGGY 2 cut(s) 3172, 5037
BsrGI TGTACA 1 cut(s) 4555
BssAI RCCGGY 2 cut(s) 3172, 5037
BssNAI GTATAC 1 cut(s) 1210
BssT1I CCWWGG 7 cut(s) 261, 941, 1736, 2321, 3759, 4160, 4317
Bst1107I GTATAC 1 cut(s) 1210
Bst4CI ACNGT 8 cut(s) 1106, 1774, 2798, 3078, 3333, 3454, 3514, 5426
Bst6I CTCTTC 7 cut(s) 110, 961, 2839, 3630, 3814, 3957, 4271
BstAPI GCANNNNNTGC 4 cut(s) 719, 1826, 3524, 4643
BstAUI TGTACA 1 cut(s) 4555
BstBAI YACGTR 2 cut(s) 3710, 4750
BstBI TTCGAA 1 cut(s) 1520
BstC8I GCNNGC 9 cut(s) 581, 872, 1467, 1703, 3672, 4168, 4848, 4975, 5039
BstDSI CCRYGG 4 cut(s) 261, 1033, 1069, 1736
BstENI CCTNNNNNAGG 3 cut(s) 2248, 3020, 3225
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 4 cut(s) 71, 197, 618, 5186
BstMAI GTCTC 9 cut(s) 34, 93, 1239, 1840, 3129, 3503, 4217, 4371, 4893
BstNSI RCATGY 5 cut(s) 874, 1705, 2953, 3599, 4016
BstPAI GACNNNNGTC 1 cut(s) 2521
BstSFI CTRYAG 2 cut(s) 1128, 2669
BstSLI GKGCMC 8 cut(s) 724, 1315, 1381, 1822, 2221, 3268, 4810, 4922
BstUI CGCG 1 cut(s) 170
BstV2I GAAGAC 3 cut(s) 1461, 1610, 3531
BstX2I RGATCY 9 cut(s) 321, 1855, 1930, 2032, 2110, 2662, 2851, 3118, 5562
BstXI CCANNNNNNTGG 3 cut(s) 2503, 3454, 3932
BstYI RGATCY 9 cut(s) 321, 1855, 1930, 2032, 2110, 2662, 2851, 3118, 5562
BstZ17I GTATAC 1 cut(s) 1210
Bsu15I ATCGAT 2 cut(s) 51, 253
BsuI GTATCC 1 cut(s) 2233
BsuTUI ATCGAT 2 cut(s) 51, 253
BtgI CCRYGG 4 cut(s) 261, 1033, 1069, 1736
BtrI CACGTC 2 cut(s) 716, 1836
BtsI GCAGTG 1 cut(s) 3999
BveI ACCTGC 2 cut(s) 4045, 5399
Cac8I GCNNGC 9 cut(s) 581, 872, 1467, 1703, 3672, 4168, 4848, 4975, 5039
CaiI CAGNNNCTG 5 cut(s) 2351, 3431, 3524, 3914, 4394
CciI TCATGA 7 cut(s) 505, 571, 1543, 1780, 2692, 3322, 5550
CfoI GCGC 4 cut(s) 71, 197, 618, 5186
Cfr10I RCCGGY 2 cut(s) 3172, 5037
ClaI ATCGAT 2 cut(s) 51, 253
CseI GACGC 2 cut(s) 75, 4196
CspCI CAANNNNNGTGG 2 cut(s) 5455, 5490
DraI TTTAAA 1 cut(s) 3534
DraIII CACNNNGTG 2 cut(s) 719, 2165
EaeI YGGCCR 5 cut(s) 264, 1066, 1884, 2493, 4176
Eam1104I CTCTTC 7 cut(s) 110, 961, 2839, 3630, 3814, 3957, 4271
EarI CTCTTC 7 cut(s) 110, 961, 2839, 3630, 3814, 3957, 4271
Ecl136II GAGCTC 2 cut(s) 1536, 3196
Eco130I CCWWGG 7 cut(s) 261, 941, 1736, 2321, 3759, 4160, 4317
Eco24I GRGCYC 6 cut(s) 1538, 1747, 3198, 3268, 4283, 4922
Eco32I GATATC 1 cut(s) 4708
Eco47I GGWCC 7 cut(s) 738, 806, 1655, 3329, 3402, 4662, 5460
Eco53kI GAGCTC 2 cut(s) 1536, 3196
Eco57I CTGAAG 8 cut(s) 175, 1653, 3116, 3708, 3723, 3891, 4140, 5004
Eco72I CACGTG 1 cut(s) 4750
EcoICRI GAGCTC 2 cut(s) 1536, 3196
EcoNI CCTNNNNNAGG 3 cut(s) 2248, 3020, 3225
EcoO109I RGGNCCY 9 cut(s) 806, 1655, 2054, 3264, 3265, 3402, 4595, 4918, 5460
EcoRV GATATC 1 cut(s) 4708
EcoT14I CCWWGG 7 cut(s) 261, 941, 1736, 2321, 3759, 4160, 4317
EcoT22I ATGCAT 1 cut(s) 3112
EcoT38I GRGCYC 6 cut(s) 1538, 1747, 3198, 3268, 4283, 4922
ErhI CCWWGG 7 cut(s) 261, 941, 1736, 2321, 3759, 4160, 4317
Esp3I CGTCTC 5 cut(s) 93, 1239, 3129, 4371, 4893
FalI AAGNNNNNCTT 2 cut(s) 193, 225
FaqI GGGAC 7 cut(s) 611, 653, 1658, 3342, 3415, 4109, 5212
FauI CCCGC 7 cut(s) 296, 322, 382, 576, 3053, 3929, 4805
FbaI TGATCA 3 cut(s) 2695, 5377, 5446
FblI GTMKAC 2 cut(s) 1209, 3217
FriOI GRGCYC 6 cut(s) 1538, 1747, 3198, 3268, 4283, 4922
FspBI CTAG 9 cut(s) 1023, 1683, 1964, 2196, 2805, 4323, 5187, 5241, 5581
FspI TGCGCA 1 cut(s) 617
GlaI GCGC 4 cut(s) 70, 196, 617, 5185
GsaI CCCAGC 1 cut(s) 4783
GsuI CTGGAG 3 cut(s) 426, 1977, 4638
HapII CCGG 9 cut(s) 282, 967, 1001, 1706, 2006, 2094, 3173, 5038, 5545
HgaI GACGC 2 cut(s) 75, 4196
HhaI GCGC 4 cut(s) 71, 197, 618, 5186
Hin6I GCGC 4 cut(s) 69, 195, 616, 5184
HinP1I GCGC 4 cut(s) 69, 195, 616, 5184
HincII GTYRAC 3 cut(s) 1009, 2794, 3967
HindII GTYRAC 3 cut(s) 1009, 2794, 3967
HindIII AAGCTT 3 cut(s) 1093, 1439, 4679
HpaII CCGG 9 cut(s) 282, 967, 1001, 1706, 2006, 2094, 3173, 5038, 5545
Hpy99I CGWCG 2 cut(s) 678, 720
HpyCH4III ACNGT 8 cut(s) 1106, 1774, 2798, 3078, 3333, 3454, 3514, 5426
HpyCH4IV ACGT 6 cut(s) 65, 715, 1835, 3709, 4749, 5532
HpySE526I ACGT 6 cut(s) 65, 715, 1835, 3709, 4749, 5532
HspAI GCGC 4 cut(s) 69, 195, 616, 5184
KflI GGGWCCC 1 cut(s) 3402
KroI GCCGGC 1 cut(s) 5037
KroNI GCCGGC 1 cut(s) 5039
Ksp22I TGATCA 3 cut(s) 2695, 5377, 5446
LguI GCTCTTC 3 cut(s) 110, 3630, 4271
LweI GCATC 9 cut(s) 411, 460, 3119, 3146, 3475, 3536, 3931, 4207, 5116
MaeI CTAG 9 cut(s) 1023, 1683, 1964, 2196, 2805, 4323, 5187, 5241, 5581
MaeII ACGT 6 cut(s) 65, 715, 1835, 3709, 4749, 5532
MflI RGATCY 9 cut(s) 321, 1855, 1930, 2032, 2110, 2662, 2851, 3118, 5562
MlsI TGGCCA 2 cut(s) 2495, 4178
MluNI TGGCCA 2 cut(s) 2495, 4178
MlyI GAGTC 9 cut(s) 272, 737, 767, 845, 991, 2378, 3962, 4447, 4664
MmeI TCCRAC 9 cut(s) 656, 877, 1076, 2041, 2425, 2755, 3997, 4010, 5351
Mox20I TGGCCA 2 cut(s) 2495, 4178
Mph1103I ATGCAT 1 cut(s) 3112
MroNI GCCGGC 1 cut(s) 5037
MroXI GAANNNNTTC 5 cut(s) 1622, 2452, 3150, 3951, 5313
MscI TGGCCA 2 cut(s) 2495, 4178
MslI CAYNNNNRTG 2 cut(s) 370, 3600
Msp20I TGGCCA 2 cut(s) 2495, 4178
MspA1I CMGCKG 4 cut(s) 1465, 2521, 3643, 5342
MspI CCGG 9 cut(s) 282, 967, 1001, 1706, 2006, 2094, 3173, 5038, 5545
Mva1269I GAATGC 3 cut(s) 1723, 3112, 3703
MvnI CGCG 1 cut(s) 170
NaeI GCCGGC 1 cut(s) 5039
NciI CCSGG 2 cut(s) 968, 1707
NcoI CCATGG 2 cut(s) 261, 1736
NgoMIV GCCGGC 1 cut(s) 5037
NmeAIII GCCGAG 4 cut(s) 884, 1696, 3106, 3403
NmuCI GTSAC 9 cut(s) 304, 836, 1264, 1839, 2370, 3682, 3875, 4492, 4818
NsbI TGCGCA 1 cut(s) 617
NsiI ATGCAT 1 cut(s) 3112
NspI RCATGY 5 cut(s) 874, 1705, 2953, 3599, 4016
NspV TTCGAA 1 cut(s) 1520
PaeI GCATGC 2 cut(s) 874, 1705
PagI TCATGA 7 cut(s) 505, 571, 1543, 1780, 2692, 3322, 5550
PaqCI CACCTGC 1 cut(s) 5399
PciI ACATGT 2 cut(s) 2949, 4012
PciSI GCTCTTC 3 cut(s) 110, 3630, 4271
PctI GAATGC 3 cut(s) 1723, 3112, 3703
PdiI GCCGGC 1 cut(s) 5039
PdmI GAANNNNTTC 5 cut(s) 1622, 2452, 3150, 3951, 5313
PflFI GACNNNGTC 1 cut(s) 1837
PflMI CCANNNNNTGG 4 cut(s) 455, 2207, 2261, 3071
PfoI TCCNGGA 1 cut(s) 2206
PleI GAGTC 9 cut(s) 272, 737, 766, 845, 991, 2377, 3962, 4446, 4664
PmaCI CACGTG 1 cut(s) 4750
PmlI CACGTG 1 cut(s) 4750
PpsI GAGTC 9 cut(s) 272, 737, 766, 845, 991, 2377, 3962, 4446, 4664
Ppu21I YACGTR 2 cut(s) 3710, 4750
PpuMI RGGWCCY 4 cut(s) 806, 1655, 3402, 5460
PscI ACATGT 2 cut(s) 2949, 4012
PshAI GACNNNNGTC 1 cut(s) 2521
PsiI TTATAA 2 cut(s) 2028, 4893
Psp124BI GAGCTC 2 cut(s) 1538, 3198
Psp1406I AACGTT 1 cut(s) 5532
Psp5II RGGWCCY 4 cut(s) 806, 1655, 3402, 5460
PspCI CACGTG 1 cut(s) 4750
PspFI CCCAGC 1 cut(s) 4779
PspOMI GGGCCC 2 cut(s) 3264, 4918
PspPPI RGGWCCY 4 cut(s) 806, 1655, 3402, 5460
PsrI GAACNNNNNNTAC 2 cut(s) 1539, 1571
PstNI CAGNNNCTG 5 cut(s) 2351, 3431, 3524, 3914, 4394
PsuI RGATCY 9 cut(s) 321, 1855, 1930, 2032, 2110, 2662, 2851, 3118, 5562
PsyI GACNNNGTC 1 cut(s) 1837
PvuII CAGCTG 1 cut(s) 3643
RseI CAYNNNNRTG 2 cut(s) 370, 3600
SacI GAGCTC 2 cut(s) 1538, 3198
SapI GCTCTTC 3 cut(s) 110, 3630, 4271
ScaI AGTACT 3 cut(s) 2885, 4575, 5002
SchI GAGTC 9 cut(s) 272, 737, 767, 845, 991, 2378, 3962, 4447, 4664
SfaNI GCATC 9 cut(s) 411, 460, 3119, 3146, 3475, 3536, 3931, 4207, 5116
SfcI CTRYAG 2 cut(s) 1128, 2669
SfuI TTCGAA 1 cut(s) 1520
SinI GGWCC 7 cut(s) 738, 806, 1655, 3329, 3402, 4662, 5460
SmiMI CAYNNNNRTG 2 cut(s) 370, 3600
SmlI CTYRAG 7 cut(s) 745, 1383, 2245, 2784, 3225, 3395, 4788
SmoI CTYRAG 7 cut(s) 745, 1383, 2245, 2784, 3225, 3395, 4788
SphI GCATGC 2 cut(s) 874, 1705
SspI AATATT 3 cut(s) 3292, 3373, 4197
SspMI CTAG 9 cut(s) 1023, 1683, 1964, 2196, 2805, 4323, 5187, 5241, 5581
SstI GAGCTC 2 cut(s) 1538, 3198
StyI CCWWGG 7 cut(s) 261, 941, 1736, 2321, 3759, 4160, 4317
TaaI ACNGT 8 cut(s) 1106, 1774, 2798, 3078, 3333, 3454, 3514, 5426
TaiI ACGT 6 cut(s) 68, 718, 1838, 3712, 4752, 5535
TaqI TCGA 8 cut(s) 51, 253, 1237, 1520, 1571, 2515, 3544, 5574
TaqII GACCGA 1 cut(s) 755
TatI WGTACW 6 cut(s) 2883, 2946, 4009, 4555, 4573, 5000
TauI GCSGC 2 cut(s) 3491, 5390
TseFI GTSAC 9 cut(s) 304, 836, 1264, 1839, 2370, 3682, 3875, 4492, 4818
Tsp45I GTSAC 9 cut(s) 304, 836, 1264, 1839, 2370, 3682, 3875, 4492, 4818
TspGWI ACGGA 4 cut(s) 292, 921, 2526, 3111
Tth111I GACNNNGTC 1 cut(s) 1837
Van91I CCANNNNNTGG 4 cut(s) 455, 2207, 2261, 3071
VneI GTGCAC 4 cut(s) 720, 1311, 1377, 2217
VpaK11BI GGWCC 7 cut(s) 738, 806, 1655, 3329, 3402, 4662, 5460
XagI CCTNNNNNAGG 3 cut(s) 2248, 3020, 3225
XapI RAATTY 9 cut(s) 1134, 1318, 3052, 3547, 3852, 4071, 4350, 5222, 5312
XbaI TCTAGA 2 cut(s) 1963, 5240
XceI RCATGY 5 cut(s) 874, 1705, 2953, 3599, 4016
XcmI CCANNNNNNNNNTGG 2 cut(s) 732, 4013
XmiI GTMKAC 2 cut(s) 1209, 3217
XmnI GAANNNNTTC 5 cut(s) 1622, 2452, 3150, 3951, 5313
XspI CTAG 9 cut(s) 1023, 1683, 1964, 2196, 2805, 4323, 5187, 5241, 5581
ZrmI AGTACT 3 cut(s) 2885, 4575, 5002
Zsp2I ATGCAT 1 cut(s) 3112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.