Rroxscaffold_7G00167360

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008668
Physical Location & Seq
Forward (+)
8503103 .. 8511038
7936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167360.1

Sequence Viewer

Length: 4476 bp
ATGAACAGTGTTCCTACAATTTCTCAGTCTATAGGAATTTCGGACAAAGTTGAAATTGAACATGGAGAACCTTACCCTTTTAGGTATAGTAGGACAAAGCTGCTTGATGTATACAGGACGGCTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCTGAGGAAATGGCTCTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACTGCTGGCAAATACTGATTCTAAAGATGAACACATTGTTAGTTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGAAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGGTGAAGCTCTTAGAGACGATGGAGGTCCTTTCAGAAAGGCTGAGGAGGCACCTAGTAGTAGCGAATTGAGCATGCCGGGAGGAGTGAATGCTCATTCTGGCACTCCATGGAGGGCTCCATCACAGGTAGAACGGTCAAATACGGTCTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGCACACCCCCTGCTATGACGTGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCCGATCCATCTTACACCAGAAATGACGCAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTCTAGATAGGGAACAGGAAATGAGAAAACCTCAGCAGCCGTCACCGGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGTCCGGATATTTCGGCATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTCAAGTAGGCCAAACCTTGGTAATGTTGGGAAGATTCATGCTGGTTTAAGTGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCACTGATGTCTGGGAATACGAGTGTTTCTACTCATCAGAAGTTTCCTTTTTCTGAAGGTTTGCAAGGGTTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGAACGACAGCGGTCAATATCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCATAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCCAGGTGGGTCAGATCCACTACAGAGTAAGATAGACATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCTTTTCCAAATCTACTAGCTCAAGCTGTTGACAGTTCATCTAGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCGTTGTTGGACAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTAATTAGGCAACAACAGCAGCTCCTTTCCCAGGTTCTTGCGGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCGTCTCGGCTTCAGGCATCACAAGAAATGTTTCCACTTGGTACAAATGTGCCAGTTCCTAATATGCAAAATGAGCTCACTACTAAGTTTATGGGTCTGCCTCCTCAAGGCACCCAAGATATTCGTCATAATGTAAGTGATGGGGCCCCTTCTTTACCTCTACCACAGAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCATTTAATGATGTCCATCAAGATTCATTGCCAGTGTCAAATATTGCCGAGAGATCACCCTTACTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTGGTTTCTGAGACAATTGCAGATTCTGCATCTTTAAATTCTCCTCGAAGTTTTATTTCTATGCCACCAGGAGCTCGGGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCAATTAGATGGTCAGGTTGAAGAACAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGCAGTGACTGAAATAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCAGAGAAGAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGACCTCAATTCAGGAATTTCTCCACAAAAGACAAGAGGTAACAGAGGCAGTAAATCTGAAATTGGTGCTTCGGAGGCTGTGCAATCCCAACAAGGTGGCGGGGATACTGAATCTCTTCAAGTCAAGGTTGATTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTAAATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTCAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAGTGGTAGTATCTGAAGTCCTCAATTCTGTCAATTCGCCAAGTTTATCAACTCCTTGGGCTGGCGTTGTGGCCAATTCAGACACAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAATGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTTTGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTTGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAAGTTATGCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGGTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAAGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCAAACGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACCAACATCTTTAAGAGATATCCAAAAGGAGCAAGGGAAGAGGGTTTCCTCTGGTTCACAGATTACAACTCCTCAGAAATCCCAGCCAACTCAAGCCACCCGCAATGGTGCCCCTTCATGGTCACTTTCAGGATCATCTCCATCTAAGCCTGCTTCTCCAATCCAGATCAATTCCCATACTTCTCAGTCAAAGTCTAAAGGAGACGATGACCTATTCTGGGGCCCAATTGACCAATCAAAGCAAGAAACCAAACAGGCCGATTTTCCTCAGCTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGTGAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAATCCGCCAGTGGCAAAGGAACTGAGCGATTACTTTCATCTTCACCGGCCTCTACTCAGTCCTCTCTGAAAGGAAAAAGAGATGCCATGACTAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCTAGGCTAATTGGGACAAAAGATACGAGTTTCCTTGAATTTTGCTTGAAGCAATCCAGAGCTGAGGCTGAGTTGCTTCTGATAGAGAATCTTGGGTCTTACGATCCTGATCATGAGTTCATTGAAAAATTTCTCAACTACAAGGAGTTGCTATCAGCGGATGTCCTTGACATAGCTTTTCAAAGTCGGAATGATCAAAAGGCCACTGGATTCAGTGGTGCAGGTGTCAATTCCTACGGTGCTGATGTTGGGGATGTTGATCAAGATGGTGGGTCCTCCAAAGGGGGTGGCAAGAAGAAGGGGAAGAAAGGGAAGAAGGTTAGTCCTGCCGTCTTGGGATTTAACGTTGTTAGTAACCGGATCATGATGGGTGAGATCCAGTCAGTCGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1491

Amino Acids

162.47

Weight (kDa)

6.03

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 310 - 354 3.1e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
AarI CACCTGC 1 cut(s) 4292
Acc36I ACCTGC 2 cut(s) 2950, 4292
AccB1I GGYRCC 4 cut(s) 580, 1813, 2135, 3701
AccB7I CCANNNNNTGG 3 cut(s) 1112, 1166, 1976
AccI GTMKAC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 998
AciI CCGC 8 cut(s) 1426, 1937, 1951, 2393, 2841, 3694, 3945, 4238
AclI AACGTT 1 cut(s) 4425
AcoI YGGCCR 3 cut(s) 786, 1398, 3081
AcsI RAATTY 9 cut(s) 36, 220, 1957, 2440, 2757, 2976, 3255, 4118, 4208
AcuI CTGAAG 6 cut(s) 1350, 2021, 2628, 3030, 3045, 3900
AdeI CACNNNGTG 1 cut(s) 1070
AfaI GTAC 8 cut(s) 1790, 1853, 2068, 2613, 3297, 3462, 3480, 3898
AflIII ACRYGT 2 cut(s) 1590, 1854
AjiI CACGTC 1 cut(s) 738
AjnI CCWGG 9 cut(s) 1111, 1555, 1809, 1926, 2470, 2685, 2947, 3154, 3873
AjuI GAANNNNNNNTTGG 2 cut(s) 3129, 3161
AloI GAACNNNNNNTCC 2 cut(s) 3504, 3536
Alw21I GWGCWC 8 cut(s) 217, 283, 440, 1042, 1126, 2103, 2367, 2479
Alw26I GTCTC 8 cut(s) 141, 540, 742, 2034, 2408, 3122, 3276, 3789
Alw44I GTGCAC 4 cut(s) 213, 279, 1038, 1122
AlwNI CAGNNNCTG 5 cut(s) 1256, 2336, 2429, 2591, 3299
Ama87I CYCGRG 1 cut(s) 2478
Aor13HI TCCGGA 1 cut(s) 998
ApaI GGGCCC 2 cut(s) 2173, 3818
ApaLI GTGCAC 4 cut(s) 213, 279, 1038, 1122
ApoI RAATTY 9 cut(s) 36, 220, 1957, 2440, 2757, 2976, 3255, 4118, 4208
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 5 cut(s) 524, 1357, 2055, 2856, 4209
AspLEI GCGC 1 cut(s) 4082
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 8 cut(s) 545, 900, 1044, 1082, 2283, 3391, 3975, 4463
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 2478
AvaII GGWCC 6 cut(s) 557, 1553, 2234, 2307, 3567, 4353
BaeGI GKGCMC 7 cut(s) 217, 283, 1042, 1126, 2173, 3706, 3818
BaeI ACNNNNGTAYC 2 cut(s) 2058, 2091
BalI TGGCCA 2 cut(s) 1400, 3083
BanI GGYRCC 4 cut(s) 580, 1813, 2135, 3701
BanII GRGCYC 7 cut(s) 440, 649, 2103, 2173, 2479, 3188, 3818
BbsI GAAGAC 2 cut(s) 363, 512
Bbv12I GWGCWC 8 cut(s) 217, 283, 440, 1042, 1126, 2103, 2367, 2479
BbvCI CCTCAGC 4 cut(s) 573, 897, 3861, 4143
BceAI ACGGC 4 cut(s) 135, 889, 3253, 4394
BcgI CGANNNNNNTGC 2 cut(s) 4047, 4081
BciT130I CCWGG 9 cut(s) 1113, 1557, 1811, 1928, 2472, 2687, 2949, 3156, 3875
BciVI GTATCC 2 cut(s) 1138, 2839
BclI TGATCA 4 cut(s) 1600, 4189, 4273, 4339
BcnI CCSGG 1 cut(s) 609
BcoDI GTCTC 8 cut(s) 141, 540, 742, 2034, 2408, 3122, 3276, 3789
BfaI CTAG 8 cut(s) 585, 869, 1101, 1685, 1710, 3228, 4083, 4474
BfmI CTRYAG 3 cut(s) 30, 1574, 2583
BfuAI ACCTGC 2 cut(s) 2950, 4292
BfuI GTATCC 2 cut(s) 1138, 2839
BglII AGATCT 1 cut(s) 1015
BmcAI AGTACT 3 cut(s) 1790, 3480, 3898
Bme18I GGWCC 6 cut(s) 557, 1553, 2234, 2307, 3567, 4353
BmeT110I CYCGRG 1 cut(s) 2478
BmgBI CACGTC 1 cut(s) 738
BmrI ACTGGG 3 cut(s) 305, 781, 3398
BmsI GCATC 6 cut(s) 2024, 2051, 2380, 2441, 3112, 4012
BmuI ACTGGG 3 cut(s) 305, 781, 3398
BoxI GACNNNNGTC 1 cut(s) 1426
BpiI GAAGAC 2 cut(s) 363, 512
BplI GAGNNNNNCTC 6 cut(s) 121, 153, 427, 459, 880, 912
BpmI CTGGAG 2 cut(s) 882, 3543
Bpu10I CCTNAGC 5 cut(s) 573, 897, 2496, 3861, 4143
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 6 cut(s) 270, 1135, 1674, 2115, 2321, 3669
BpuMI CCSGG 1 cut(s) 609
BsaAI YACGTR 1 cut(s) 2615
BsaBI GATNNNNATC 3 cut(s) 2250, 4188, 4338
BsaJI CCNNGG 9 cut(s) 638, 1165, 1226, 1809, 1926, 2664, 3065, 3222, 3874
BsaWI WCCGGW 3 cut(s) 910, 998, 4437
Bse118I RCCGGY 2 cut(s) 3933, 3985
Bse3DI GCAATG 4 cut(s) 2261, 2575, 3703, 4053
Bse8I GATNNNNATC 3 cut(s) 2250, 4188, 4338
BseAI TCCGGA 1 cut(s) 998
BseBI CCWGG 9 cut(s) 1113, 1557, 1811, 1928, 2472, 2687, 2949, 3156, 3875
BseDI CCNNGG 9 cut(s) 638, 1165, 1226, 1809, 1926, 2664, 3065, 3222, 3874
BseGI GGATG 5 cut(s) 979, 2325, 3310, 4246, 4339
BseJI GATNNNNATC 3 cut(s) 2250, 4188, 4338
BseMI GCAATG 4 cut(s) 2261, 2575, 3703, 4053
BseRI GAGGAG 9 cut(s) 432, 590, 627, 1614, 1901, 2118, 2436, 3224, 3654
BseSI GKGCMC 7 cut(s) 217, 283, 1042, 1126, 2173, 3706, 3818
BseYI CCCAGC 1 cut(s) 3675
BsgI GTGCAG 4 cut(s) 2336, 2921, 3252, 4320
BshNI GGYRCC 4 cut(s) 580, 1813, 2135, 3701
BsiHKAI GWGCWC 8 cut(s) 217, 283, 440, 1042, 1126, 2103, 2367, 2479
BsiHKCI CYCGRG 1 cut(s) 2478
BsiSI CCGG 6 cut(s) 608, 911, 999, 3934, 3986, 4438
BslFI GGGAC 3 cut(s) 2247, 2320, 4108
BsmAI GTCTC 8 cut(s) 141, 540, 742, 2034, 2408, 3122, 3276, 3789
BsmBI CGTCTC 5 cut(s) 141, 540, 2034, 3276, 3789
BsmFI GGGAC 3 cut(s) 2247, 2320, 4108
BsmI GAATGC 3 cut(s) 625, 2017, 2608
BsoBI CYCGRG 1 cut(s) 2478
Bsp119I TTCGAA 1 cut(s) 422
Bsp120I GGGCCC 2 cut(s) 2169, 3814
Bsp13I TCCGGA 1 cut(s) 998
Bsp1407I TGTACA 1 cut(s) 3460
Bsp19I CCATGG 1 cut(s) 638
BspACI CCGC 8 cut(s) 1426, 1937, 1951, 2393, 2841, 3694, 3945, 4238
BspEI TCCGGA 1 cut(s) 998
BspHI TCATGA 6 cut(s) 445, 682, 1597, 2227, 4192, 4443
BspMAI CTGCAG 1 cut(s) 2587
BspMI ACCTGC 2 cut(s) 2950, 4292
BspQI GCTCTTC 1 cut(s) 3176
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 4 cut(s) 580, 1813, 2135, 3701
BsrDI GCAATG 4 cut(s) 2261, 2575, 3703, 4053
BsrFI RCCGGY 2 cut(s) 3933, 3985
BsrGI TGTACA 1 cut(s) 3460
BssAI RCCGGY 2 cut(s) 3933, 3985
BssECI CCNNGG 9 cut(s) 638, 1165, 1226, 1809, 1926, 2664, 3065, 3222, 3874
BssNAI GTATAC 1 cut(s) 112
BssT1I CCWWGG 6 cut(s) 638, 1165, 1226, 2664, 3065, 3222
Bst1107I GTATAC 1 cut(s) 112
Bst2UI CCWGG 9 cut(s) 1113, 1557, 1811, 1928, 2472, 2687, 2949, 3156, 3875
Bst4CI ACNGT 7 cut(s) 8, 666, 676, 1703, 1983, 2359, 4319
Bst6I CTCTTC 5 cut(s) 1744, 2719, 2862, 3176, 3626
BstAPI GCANNNNNTGC 2 cut(s) 2429, 3548
BstAUI TGTACA 1 cut(s) 3460
BstBAI YACGTR 1 cut(s) 2615
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 8 cut(s) 369, 605, 2577, 3073, 3744, 3867, 3871, 3935
BstDSI CCRYGG 1 cut(s) 638
BstENI CCTNNNNNAGG 3 cut(s) 1153, 1925, 2130
BstF5I GGATG 5 cut(s) 979, 2325, 3310, 4246, 4339
BstHHI GCGC 1 cut(s) 4082
BstMAI GTCTC 8 cut(s) 141, 540, 742, 2034, 2408, 3122, 3276, 3789
BstNI CCWGG 9 cut(s) 1113, 1557, 1811, 1928, 2472, 2687, 2949, 3156, 3875
BstNSI RCATGY 4 cut(s) 607, 1594, 1858, 2504
BstPAI GACNNNNGTC 1 cut(s) 1426
BstSFI CTRYAG 3 cut(s) 30, 1574, 2583
BstSLI GKGCMC 7 cut(s) 217, 283, 1042, 1126, 2173, 3706, 3818
BstV2I GAAGAC 2 cut(s) 363, 512
BstX2I RGATCY 8 cut(s) 757, 835, 937, 1015, 1567, 1756, 2023, 4455
BstXI CCANNNNNNTGG 3 cut(s) 1408, 2359, 2837
BstYI RGATCY 8 cut(s) 757, 835, 937, 1015, 1567, 1756, 2023, 4455
BstZ17I GTATAC 1 cut(s) 112
BsuI GTATCC 2 cut(s) 1138, 2839
BtgI CCRYGG 1 cut(s) 638
BtrI CACGTC 1 cut(s) 738
BtsCI GGATG 5 cut(s) 979, 2325, 3310, 4246, 4339
BtsI GCAGTG 2 cut(s) 2592, 2904
BveI ACCTGC 2 cut(s) 2950, 4292
Cac8I GCNNGC 8 cut(s) 369, 605, 2577, 3073, 3744, 3867, 3871, 3935
CaiI CAGNNNCTG 5 cut(s) 1256, 2336, 2429, 2591, 3299
CciI TCATGA 6 cut(s) 445, 682, 1597, 2227, 4192, 4443
CfoI GCGC 1 cut(s) 4082
Cfr10I RCCGGY 2 cut(s) 3933, 3985
CseI GACGC 1 cut(s) 821
Csp6I GTAC 8 cut(s) 1789, 1852, 2067, 2612, 3296, 3461, 3479, 3897
CspCI CAANNNNNGTGG 4 cut(s) 2385, 2420, 4348, 4383
CviQI GTAC 8 cut(s) 1789, 1852, 2067, 2612, 3296, 3461, 3479, 3897
DraI TTTAAA 1 cut(s) 2439
DraIII CACNNNGTG 1 cut(s) 1070
EaeI YGGCCR 3 cut(s) 786, 1398, 3081
Eam1104I CTCTTC 5 cut(s) 1744, 2719, 2862, 3176, 3626
EarI CTCTTC 5 cut(s) 1744, 2719, 2862, 3176, 3626
EciI GGCGGA 1 cut(s) 3934
Ecl136II GAGCTC 3 cut(s) 438, 2101, 2477
Eco130I CCWWGG 6 cut(s) 638, 1165, 1226, 2664, 3065, 3222
Eco24I GRGCYC 7 cut(s) 440, 649, 2103, 2173, 2479, 3188, 3818
Eco32I GATATC 1 cut(s) 3613
Eco47I GGWCC 6 cut(s) 557, 1553, 2234, 2307, 3567, 4353
Eco53kI GAGCTC 3 cut(s) 438, 2101, 2477
Eco57I CTGAAG 6 cut(s) 1350, 2021, 2628, 3030, 3045, 3900
Eco88I CYCGRG 1 cut(s) 2478
EcoICRI GAGCTC 3 cut(s) 438, 2101, 2477
EcoNI CCTNNNNNAGG 3 cut(s) 1153, 1925, 2130
EcoO109I RGGNCCY 8 cut(s) 557, 959, 2169, 2170, 2307, 3500, 3814, 4353
EcoRII CCWGG 9 cut(s) 1111, 1555, 1809, 1926, 2470, 2685, 2947, 3154, 3873
EcoRV GATATC 1 cut(s) 3613
EcoT14I CCWWGG 6 cut(s) 638, 1165, 1226, 2664, 3065, 3222
EcoT22I ATGCAT 1 cut(s) 2017
EcoT38I GRGCYC 7 cut(s) 440, 649, 2103, 2173, 2479, 3188, 3818
ErhI CCWWGG 6 cut(s) 638, 1165, 1226, 2664, 3065, 3222
Esp3I CGTCTC 5 cut(s) 141, 540, 2034, 3276, 3789
FaqI GGGAC 3 cut(s) 2247, 2320, 4108
FauI CCCGC 3 cut(s) 1958, 2834, 3701
FbaI TGATCA 4 cut(s) 1600, 4189, 4273, 4339
FblI GTMKAC 1 cut(s) 111
FokI GGATG 5 cut(s) 986, 2312, 3317, 4253, 4346
FriOI GRGCYC 7 cut(s) 440, 649, 2103, 2173, 2479, 3188, 3818
FspBI CTAG 8 cut(s) 585, 869, 1101, 1685, 1710, 3228, 4083, 4474
GlaI GCGC 1 cut(s) 4081
GsaI CCCAGC 1 cut(s) 3679
GsuI CTGGAG 2 cut(s) 882, 3543
HapII CCGG 6 cut(s) 608, 911, 999, 3934, 3986, 4438
HgaI GACGC 1 cut(s) 821
HhaI GCGC 1 cut(s) 4082
Hin6I GCGC 1 cut(s) 4080
HinP1I GCGC 1 cut(s) 4080
HincII GTYRAC 1 cut(s) 1699
HindII GTYRAC 1 cut(s) 1699
HindIII AAGCTT 2 cut(s) 341, 3584
HpaII CCGG 6 cut(s) 608, 911, 999, 3934, 3986, 4438
HphI GGTGA 8 cut(s) 545, 900, 1044, 1082, 2283, 3391, 3975, 4463
Hpy166II GTNNAC 9 cut(s) 112, 215, 281, 321, 1040, 1124, 1699, 3570, 3650
Hpy8I GTNNAC 9 cut(s) 112, 215, 281, 321, 1040, 1124, 1699, 3570, 3650
HpyCH4III ACNGT 7 cut(s) 8, 666, 676, 1703, 1983, 2359, 4319
HpyCH4IV ACGT 3 cut(s) 737, 2614, 4425
HpySE526I ACGT 3 cut(s) 737, 2614, 4425
HspAI GCGC 1 cut(s) 4080
KflI GGGWCCC 1 cut(s) 2307
Kpn2I TCCGGA 1 cut(s) 998
KroI GCCGGC 1 cut(s) 3933
KroNI GCCGGC 1 cut(s) 3935
Ksp22I TGATCA 4 cut(s) 1600, 4189, 4273, 4339
LguI GCTCTTC 1 cut(s) 3176
LweI GCATC 6 cut(s) 2024, 2051, 2380, 2441, 3112, 4012
MaeI CTAG 8 cut(s) 585, 869, 1101, 1685, 1710, 3228, 4083, 4474
MaeII ACGT 3 cut(s) 737, 2614, 4425
MfeI CAATTG 2 cut(s) 2418, 3819
MflI RGATCY 8 cut(s) 757, 835, 937, 1015, 1567, 1756, 2023, 4455
MlsI TGGCCA 2 cut(s) 1400, 3083
MluNI TGGCCA 2 cut(s) 1400, 3083
MlyI GAGTC 4 cut(s) 1004, 1283, 3352, 3569
MmeI TCCRAC 7 cut(s) 946, 1330, 1660, 1824, 2902, 2915, 4247
Mox20I TGGCCA 2 cut(s) 1400, 3083
Mph1103I ATGCAT 1 cut(s) 2017
MroI TCCGGA 1 cut(s) 998
MroNI GCCGGC 1 cut(s) 3933
MroXI GAANNNNTTC 5 cut(s) 524, 1357, 2055, 2856, 4209
MscI TGGCCA 2 cut(s) 1400, 3083
MslI CAYNNNNRTG 1 cut(s) 2505
Msp20I TGGCCA 2 cut(s) 1400, 3083
MspA1I CMGCKG 4 cut(s) 1426, 2548, 3865, 4238
MspI CCGG 6 cut(s) 608, 911, 999, 3934, 3986, 4438
MunI CAATTG 2 cut(s) 2418, 3819
Mva1269I GAATGC 3 cut(s) 625, 2017, 2608
MvaI CCWGG 9 cut(s) 1113, 1557, 1811, 1928, 2472, 2687, 2949, 3156, 3875
NaeI GCCGGC 1 cut(s) 3935
NciI CCSGG 1 cut(s) 609
NcoI CCATGG 1 cut(s) 638
NgoMIV GCCGGC 1 cut(s) 3933
NmeAIII GCCGAG 2 cut(s) 2011, 2308
NmuCI GTSAC 7 cut(s) 166, 741, 906, 1275, 2587, 3397, 3714
NsiI ATGCAT 1 cut(s) 2017
NspI RCATGY 4 cut(s) 607, 1594, 1858, 2504
NspV TTCGAA 1 cut(s) 422
PaeI GCATGC 1 cut(s) 607
PagI TCATGA 6 cut(s) 445, 682, 1597, 2227, 4192, 4443
PaqCI CACCTGC 1 cut(s) 4292
PciI ACATGT 2 cut(s) 1590, 1854
PciSI GCTCTTC 1 cut(s) 3176
PctI GAATGC 3 cut(s) 625, 2017, 2608
PdiI GCCGGC 1 cut(s) 3935
PdmI GAANNNNTTC 5 cut(s) 524, 1357, 2055, 2856, 4209
PflFI GACNNNGTC 1 cut(s) 739
PflMI CCANNNNNTGG 3 cut(s) 1112, 1166, 1976
PfoI TCCNGGA 1 cut(s) 1111
PleI GAGTC 4 cut(s) 1003, 1282, 3351, 3569
PpsI GAGTC 4 cut(s) 1003, 1282, 3351, 3569
Ppu21I YACGTR 1 cut(s) 2615
PpuMI RGGWCCY 3 cut(s) 557, 2307, 4353
PscI ACATGT 2 cut(s) 1590, 1854
PshAI GACNNNNGTC 1 cut(s) 1426
PshBI ATTAAT 1 cut(s) 1898
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 3 cut(s) 440, 2103, 2479
Psp1406I AACGTT 1 cut(s) 4425
Psp5II RGGWCCY 3 cut(s) 557, 2307, 4353
Psp6I CCWGG 9 cut(s) 1111, 1555, 1809, 1926, 2470, 2685, 2947, 3154, 3873
PspFI CCCAGC 1 cut(s) 3675
PspGI CCWGG 9 cut(s) 1111, 1555, 1809, 1926, 2470, 2685, 2947, 3154, 3873
PspOMI GGGCCC 2 cut(s) 2169, 3814
PspPPI RGGWCCY 3 cut(s) 557, 2307, 4353
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstI CTGCAG 1 cut(s) 2587
PstNI CAGNNNCTG 5 cut(s) 1256, 2336, 2429, 2591, 3299
PsuI RGATCY 8 cut(s) 757, 835, 937, 1015, 1567, 1756, 2023, 4455
PsyI GACNNNGTC 1 cut(s) 739
PvuII CAGCTG 2 cut(s) 2548, 3865
RsaI GTAC 8 cut(s) 1790, 1853, 2068, 2613, 3297, 3462, 3480, 3898
RsaNI GTAC 8 cut(s) 1789, 1852, 2067, 2612, 3296, 3461, 3479, 3897
RseI CAYNNNNRTG 1 cut(s) 2505
SacI GAGCTC 3 cut(s) 440, 2103, 2479
SapI GCTCTTC 1 cut(s) 3176
ScaI AGTACT 3 cut(s) 1790, 3480, 3898
SchI GAGTC 4 cut(s) 1004, 1283, 3352, 3569
SfaNI GCATC 6 cut(s) 2024, 2051, 2380, 2441, 3112, 4012
SfcI CTRYAG 3 cut(s) 30, 1574, 2583
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 6 cut(s) 557, 1553, 2234, 2307, 3567, 4353
SmiMI CAYNNNNRTG 1 cut(s) 2505
SmlI CTYRAG 6 cut(s) 285, 1150, 1689, 2130, 2300, 3684
SmoI CTYRAG 6 cut(s) 285, 1150, 1689, 2130, 2300, 3684
SphI GCATGC 1 cut(s) 607
SsiI CCGC 8 cut(s) 1426, 1937, 1951, 2393, 2841, 3694, 3945, 4238
SspI AATATT 3 cut(s) 2197, 2278, 3102
SspMI CTAG 8 cut(s) 585, 869, 1101, 1685, 1710, 3228, 4083, 4474
SstI GAGCTC 3 cut(s) 440, 2103, 2479
StyI CCWWGG 6 cut(s) 638, 1165, 1226, 2664, 3065, 3222
TaaI ACNGT 7 cut(s) 8, 666, 676, 1703, 1983, 2359, 4319
TaiI ACGT 3 cut(s) 740, 2617, 4428
TaqI TCGA 5 cut(s) 139, 422, 473, 2449, 4467
TatI WGTACW 5 cut(s) 1788, 1851, 3460, 3478, 3896
TauI GCSGC 1 cut(s) 2396
TseFI GTSAC 7 cut(s) 166, 741, 906, 1275, 2587, 3397, 3714
Tsp45I GTSAC 7 cut(s) 166, 741, 906, 1275, 2587, 3397, 3714
TspGWI ACGGA 2 cut(s) 1431, 2016
Tth111I GACNNNGTC 1 cut(s) 739
Van91I CCANNNNNTGG 3 cut(s) 1112, 1166, 1976
VneI GTGCAC 4 cut(s) 213, 279, 1038, 1122
VpaK11BI GGWCC 6 cut(s) 557, 1553, 2234, 2307, 3567, 4353
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 3 cut(s) 1153, 1925, 2130
XapI RAATTY 9 cut(s) 36, 220, 1957, 2440, 2757, 2976, 3255, 4118, 4208
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 4 cut(s) 607, 1594, 1858, 2504
XcmI CCANNNNNNNNNTGG 1 cut(s) 2918
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 5 cut(s) 524, 1357, 2055, 2856, 4209
XspI CTAG 8 cut(s) 585, 869, 1101, 1685, 1710, 3228, 4083, 4474
ZrmI AGTACT 3 cut(s) 1790, 3480, 3898
Zsp2I ATGCAT 1 cut(s) 2017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.