Rh6CG427300

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008668
Physical Location & Seq
Reverse (-)
60941210 .. 60949615
8406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG427300.1

Sequence Viewer

Length: 4815 bp
ATGCAGGAAAAACCTCTCAGCCCAAATCCATCATTTGGCAATCGCTCAGAAGCCTTGAAGTTGTCAGGAAATGGTGAGGATATTCATGATACCCAGAAGAAAAAGGATGTTTTTAGGCCATCCTTGCTGGACATGGAAACTGGTGGTCGTCATGAACGCTGGCGGGATGAAGAAAGGGACACCAATTCTGCTGTGCGCAAGGATCGGTGGAGGGATGGGGACAAAGAGCTTAATGACACTCGCAGGATGGATCGTCGGACAGAAAATGCACCAGCAAAGCACTTTGGAGAGGCACGTCGTGCACCATCAGAAAGGTGGACCGACTCAAGTAACAGGGAGTCCAATTATGAGCAACGGCGAGAGAGCAAATGGAACTCCCGTTGGGGTCCTGATAACAAGGAGGCAGAAGGTTTGCGTGACAAATGGGCTGACTCAGGCCGAGATGGCAGCATGCAGGATAAAGGCTTATCTAATGTTGGAAATCACGGAAAGGATGAGAAGGATGGGGATCACTATCGGCCTTGGAGATCAAACTCTTCCCAAATCCGGGGAAGGGGGGAGCCTTCTCATAACCAGACTCCGGCAGTCAACAAATATATTCCTAGTAGGGGCCGTGGGGAAAATACACCTCCAACCTTTTCTGTTGGCCGTGGAAGGGTTAGCCCTGGTGGAAGCTTTGTGAACAGTGTTCCTACAATTTCTCAATCTGTAGGAATTTCGGACAAAGTTGAAATTGAACATGGAGAGCCTTACCCTTTTAGGTATAGTAGGACAAAGCTGTTTGATGTATACAGGACGGCTGATATGAGATCGTCTCGAAAATTAGTTGATGGGTTTATAGATGTGACTTCTCTTACTTTGGAGGAACCATTGGAGCCTCTGGCTCTTTGTGCACCAAATTCAGAGGAAATGGCACTTTTGAAGGGAATTGACAAAGGAGATATAGTAAGTAGTGGTGCACCTCAAGTTTCCAAAGATGGAAGGAACCCAGTAGAGTTTACACAAACAAGAAGAACAAAGCTTGGAAGTAGAGAAGACATACCGCTGGCAAATACCGATTCTAAAGATGAACACATTGTTAGTTCAAAAGGTGGTTTTTCGAATTATTTAGAGAGCTCTCCTCATGAACAGCAATTACTTCATCATGGGTCGAGCCTAAAAGCCGAAGCAACGCTGGATCAGAAGACATACTCAGAGAACAGGTTCAGAGCTGAAGCTCTTAGGGACGATGGAGGTCCTTTCAGAAAGGCCGAGGAGGCACCTAGTAGTAGGGAATTGAGCATGCCGGGAGGAGTGAATGCTCATTCTGGCACTCCATGGAGGGCTCCATCACAGGTAGAACGCTCAAATACGGTTTTTCATGATTGGCAAGAGATTCCCAGAGATATGAAGTCAGGCACACCCCCTGCTATGACGTGGTCACAGAGACAGAAGGATCTAAATAATGACTGGGAAAGTAATCTGGCCGATCCATCTTACACCAGAAATGACGCAAAATGGAAAACCAGTGAAGATCCTATTATTAGAAGGCAGCTATCTGGAGTTCTAGATAGGGAACAGGAAGTAAGAAAACCTCAGCAGCCCTCACCAGAGGAACTCCAACTTTATTATAAAGATCCTCATGGTGTAATTCAAGGCCCTTTTTCTGGGGATGACATTATTGGCTGGTTTGAGGCCGGATATTTTGGCATAGATCTGCAAGTTCGTGTGGCAAGTGCACCAAATGATTCACCATTTTCATCACTTGGTGATGTTATGCCTCATTTACGAGCTAAAGCTAGGCCACCTCCTGGATTTAGTGCACCTAAAAATGAAGTTATGGATACCTCAAGTAGGCCAAACTTTGGTAATGTTGGGAAGATTCATGCTGGTTTAAGCGAGGCAGATATAGCAAGAAATGATCCAAGGCTTAAACAGAGTTCAATAACAGAAGCTGAAAATAGGTTTTTGGAGTCACTGATGTCTGGGAATATGAGTGGTTCTACTCATCAGAAGTTTCCTTTTTCTGAAGGTTTGCAAGGATTTGTTGGAAATAATTCTCATGGTATGCCTCATTCTGGATTGGAAAACCTGTTGGCCAAGAGAATGGCACTTGAACGACAGCGGTCAATACCTAATCCGTATCTTGAAAATCCCCATAACCAAAATGCTGAGATCAAATCAGTCCTTCAAGGATTAACTGATAGGTCTGGCTTAAATAATAATGCTGCTGGTTGGTCAAGTTTTTCTGGTCAAGGTGGGTCAGATCCACTACAGAGTAAGATAGATATGTATCATGATCAAAGTTTTCCTACCCAAGCTCCTCTCGGATTCCAACAACAGAGGTTGCAACCTCAAAATCAACCATCTTTTCCGAATCTACTCGCTCAAGCTGTTGACAGTTCATCTAGCATTTCAACGCAAGAGAAATTACTCTCTTCTGGCTTATTGCAAGATCCCCAACTGATGAATATATTGCAACAGCAGTACTTGTTGCAGTTACATTCCCAGGCACCTGTTCCAGCACAGCAGATGTCATTATTGGATAAGTACATGTTGATTAAGCAGCAGCAACAGAAGCAGGAGGAGGAACTATTACTTAGGCAACAACAGCAGCTCCTTTCCCAAGTTCTTGCGGATCATCAATCCCGCCAAAATTTTTCGGAGCCATCTTTTGGACAGTTACAGGCTGCTGCGATACCACAAGGGAATGCATCAATAGATCCGTCTCGGCTTCAGGCATCACAAGAAATGTTTCCAGTTGGTACAAATGTGCCGGTTCCTAATATGCAAAATGAGCTCACTACTAAGTTTATGGGTCTGCCTCCTCAAGGCACCCAAGATATTCGTCAGAATGTAAGTGATGGGGCCCCTTCTTTACCTCTACCACAAAATATTACTCATCAGAGAAGTTGGGATAATACTCATGAGGGACCGTTTAATGATGTCCATCAAGATTCATTGCCAGTGTCAAATATTGCCGAGAGATCACCCTTACTTGAGGGGACCCTTGTGCAGAAATCCATCCCAGATTCTGACTTCAATGCTGCCAAAACTGTGGAGCACACATCAGAAAAGACTTCCAGAGATGCGGCAACTGAGGTTGTTTCTGAGACAGTTGCAGATTCTGCATCTTTAAAGTCTTCTCAAAGTTTTATTCCTATGCCACCAGGAGCTTGTGAGGAAGATATGCCTGAGCATGTCAATGATGGGAAAGCTCAATTAGATAGTCAGCTTGAAGAGCAGCTGGTTGAAAAGGAAAAATGCAATGAGCAGGCTCCTGTGGTGACTGAAGTAAAGAATGCTGAAGTACGTGGGCAAAAAAAGACATCAGAGAAAAAGTCCAAGAAGCAAAAATCTTCCAAGGCACAGGCTACAAATGACCAGGCAAAAGGAGTATCAAAATCTTCCTCTTCACAGCAAATAGAGCAGTCTGAAACTGACCTCAATTCAGGAATTTCTCCTCAAAAGACAAGAGGTGACAGAGGCAGTAAATCTGAAATTGGTACTTCGGAGGCTGTGCAATCCCAACAAGGTGGCGGGGATGCTGAATCTCTTCAAGTCAAGGTTGATTCTAAACCAGTTGAGCCTGTTGCAGTGCAGAATACCCAAGTACATGTTGGACAGAGAGGTTGGAAACCTGCTCCTGGTTTTAAGGCAAAATCACTATTAGAAATTCAACAGGAAGAACAGAGGAAAGTGCAAACTGAAGTGGTAGTATCTGAAGTCCCCAATTCTGTCAATTCGCAAAGTTTATCAACTCCTTGGGCTGGCATTGTGGCCAATTCAGACCCAAAAATATTTAGAGAAAATGAGAGAGATGCAGAGACTAAAGAATTAAATGTTGGGAAACCTGGAACTTCAAATCGTAAGAGCAAGAAGAGCCCATTACATGATCTTTTGGCAGAGGAGGGTTTGTCCAAGGCTAGTGCAGTCATTGAGGTTCCTAATGGAATTTTAAGTCAGCCGTCTCCACAAGTTATTCCTCATTCAGTACCTGTGGATGATGATAACTTCATTGAGGCTAAAGACACCAAAAGGAGTCGCAAAAAGTCGGCAAAATCAAAGGGTTCTGCAACTAAGGTTTCAGGAGCAGTCACCCCAGTAGAAGTGCCTATTAGTTCAAGCCCCACTGAGAAAGTCAAAGGCTCCCGTTCTGTACAACAGGAAAAGGAAGTACTACCCACAATTCCATCAGGGCCTTCGTTGGGAGATTTTGTTCTTTGGAAGGGAGAAACAGCAAACGCTGCTCCTTCTCCAGCATGGTCCACTGACTCTGGGAAGCTTAATAAACGAACATCTTTAAGAGATATCCAAAAGGAGCAAGGGAAGAGGGTTTCCTCTGCTCAGCACGTGAACCAGATTACAACTCCTCAGAAATCCCAGCCAACACAAGCCACCCGCAATGGTGCCCCTTCATGGTCACTTTCAGGATCATCTCCATCTAATCCTGCTTCTCCAATCCAGATCAATTCCCATACTTCTCAGTCAAATTATAAAGGAGATGATGACCTATTCTGGGGCCCGATTGACCAATCAAAGCAAGAAACCAAACAGGCCGATTTTCCTCAGTTGGCAAGCCAGGGTAGTAGGGGAATGAAAAGTACTCCTGCGAAAGTAACTTCAGGTGGTTCATTAAGCCGGCAGAAATCCGCAAGTGGCAAAGGAACTGAGCGATTACTTTCATCTTCACTGGCCTCTGCTCAGTCCTCTCTGAAAGGAAAAAGAGATGCCATGACCAAGCAATCAGAAGCAATGGACTTCCGAGATTGGTGCAAGAGTGAGTGCGCTAGGCTAATTGGGACAAAAGGTATGCTTGTCTTTCTGACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1604

Amino Acids

176.43

Weight (kDa)

6.61

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 536 - 580 2.5e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1611, 4476
Acc16I TGCGCA 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 3628
AccB1I GGYRCC 4 cut(s) 1258, 2491, 2813, 4388
AccB7I CCANNNNNTGG 4 cut(s) 35, 1790, 1844, 2654
AccI GTMKAC 1 cut(s) 789
AciI CCGC 9 cut(s) 163, 1043, 2104, 2615, 2629, 3071, 3519, 4381, 4632
AcoI YGGCCR 4 cut(s) 646, 1464, 2076, 3759
AcsI RAATTY 6 cut(s) 714, 898, 2635, 3435, 3654, 3933
AcuI CTGAAG 8 cut(s) 1233, 2028, 2699, 3291, 3306, 3708, 3723, 4587
AcvI CACGTG 1 cut(s) 4333
AdeI CACNNNGTG 2 cut(s) 299, 1748
AflIII ACRYGT 2 cut(s) 2532, 3595
AjiI CACGTC 2 cut(s) 296, 1416
AjnI CCWGG 8 cut(s) 664, 1789, 2487, 3148, 3363, 3625, 3832, 4560
AjuI GAANNNNNNNTTGG 2 cut(s) 3807, 3839
AloI GAACNNNNNNTCC 2 cut(s) 4182, 4214
Alw21I GWGCWC 8 cut(s) 304, 895, 961, 1118, 1720, 1804, 2781, 3045
Alw26I GTCTC 6 cut(s) 819, 1420, 2712, 3086, 3800, 3954
Alw44I GTGCAC 5 cut(s) 300, 891, 957, 1716, 1800
AlwNI CAGNNNCTG 4 cut(s) 1934, 3014, 3107, 3977
ApaI GGGCCC 2 cut(s) 2851, 4505
ApaLI GTGCAC 5 cut(s) 300, 891, 957, 1716, 1800
ApoI RAATTY 6 cut(s) 714, 898, 2635, 3435, 3654, 3933
Asp700I GAANNNNTTC 4 cut(s) 1202, 2035, 2733, 3534
AspLEI GCGC 2 cut(s) 198, 4769
AsuC2I CCSGG 2 cut(s) 548, 1287
AsuHPI GGTGA 8 cut(s) 86, 1578, 1722, 1760, 2961, 3277, 3470, 4069
AsuII TTCGAA 1 cut(s) 1100
AvaII GGWCC 6 cut(s) 318, 386, 1235, 2912, 2985, 4245
BaeGI GKGCMC 8 cut(s) 304, 895, 961, 1720, 1804, 2851, 4393, 4505
BaeI ACNNNNGTAYC 2 cut(s) 2736, 2769
BalI TGGCCA 2 cut(s) 2078, 3761
BanI GGYRCC 4 cut(s) 1258, 2491, 2813, 4388
BanII GRGCYC 6 cut(s) 1118, 1327, 2781, 2851, 3866, 4505
BbrPI CACGTG 1 cut(s) 4333
BbsI GAAGAC 3 cut(s) 1041, 1190, 3114
Bbv12I GWGCWC 8 cut(s) 304, 895, 961, 1118, 1720, 1804, 2781, 3045
BbvCI CCTCAGC 1 cut(s) 1575
BceAI ACGGC 5 cut(s) 371, 597, 633, 813, 3931
BcgI CGANNNNNNTGC 2 cut(s) 4734, 4768
BciT130I CCWGG 8 cut(s) 666, 1791, 2489, 3150, 3365, 3627, 3834, 4562
BciVI GTATCC 1 cut(s) 1816
BclI TGATCA 1 cut(s) 2278
BcnI CCSGG 2 cut(s) 548, 1287
BcoDI GTCTC 6 cut(s) 819, 1420, 2712, 3086, 3800, 3954
BfaI CTAG 7 cut(s) 603, 1263, 1547, 1779, 2388, 3906, 4770
BfmI CTRYAG 2 cut(s) 708, 2252
BfuAI ACCTGC 1 cut(s) 3628
BfuI GTATCC 1 cut(s) 1816
BglI GCCNNNNNGGC 2 cut(s) 444, 1256
BglII AGATCT 1 cut(s) 1693
BlpI GCTNAGC 1 cut(s) 4326
BmcAI AGTACT 3 cut(s) 2468, 4158, 4585
Bme18I GGWCC 6 cut(s) 318, 386, 1235, 2912, 2985, 4245
BmgBI CACGTC 2 cut(s) 296, 1416
BmrI ACTGGG 3 cut(s) 983, 1459, 4076
BmsI GCATC 7 cut(s) 2702, 2729, 3058, 3119, 3514, 3790, 4699
BmuI ACTGGG 3 cut(s) 983, 1459, 4076
BoxI GACNNNNGTC 1 cut(s) 2104
BpiI GAAGAC 3 cut(s) 1041, 1190, 3114
BplI GAGNNNNNCTC 4 cut(s) 799, 831, 1105, 1137
BpmI CTGGAG 2 cut(s) 1560, 4221
Bpu10I CCTNAGC 2 cut(s) 1575, 3174
Bpu1102I GCTNAGC 1 cut(s) 4326
Bpu14I TTCGAA 1 cut(s) 1100
BpuEI CTTGAG 6 cut(s) 310, 948, 1813, 2352, 2793, 2999
BpuMI CCSGG 2 cut(s) 548, 1287
BsaAI YACGTR 2 cut(s) 3293, 4333
BsaBI GATNNNNATC 4 cut(s) 507, 513, 2271, 2928
Bse118I RCCGGY 2 cut(s) 2755, 4620
Bse1I ACTGG 9 cut(s) 145, 989, 1454, 1506, 2738, 2945, 3560, 4082, 4677
Bse3DI GCAATG 4 cut(s) 2939, 3253, 4390, 4740
Bse8I GATNNNNATC 4 cut(s) 507, 513, 2271, 2928
BseBI CCWGG 8 cut(s) 666, 1791, 2489, 3150, 3365, 3627, 3834, 4562
BseJI GATNNNNATC 4 cut(s) 507, 513, 2271, 2928
BseMI GCAATG 4 cut(s) 2939, 3253, 4390, 4740
BseNI ACTGG 9 cut(s) 145, 989, 1454, 1506, 2738, 2945, 3560, 4082, 4677
BseRI GAGGAG 9 cut(s) 1110, 1268, 1305, 2292, 2579, 2796, 3432, 3902, 4341
BseSI GKGCMC 8 cut(s) 304, 895, 961, 1720, 1804, 2851, 4393, 4505
BseYI CCCAGC 1 cut(s) 4362
BsgI GTGCAG 3 cut(s) 3014, 3599, 3930
BshNI GGYRCC 4 cut(s) 1258, 2491, 2813, 4388
BsiHKAI GWGCWC 8 cut(s) 304, 895, 961, 1118, 1720, 1804, 2781, 3045
BsiSI CCGG 6 cut(s) 547, 581, 1286, 1677, 2756, 4621
BslFI GGGAC 7 cut(s) 191, 233, 1238, 2925, 2998, 3692, 4795
BsmAI GTCTC 6 cut(s) 819, 1420, 2712, 3086, 3800, 3954
BsmBI CGTCTC 3 cut(s) 819, 2712, 3954
BsmFI GGGAC 7 cut(s) 191, 233, 1238, 2925, 2998, 3692, 4795
BsmI GAATGC 3 cut(s) 1303, 2695, 3286
Bsp119I TTCGAA 1 cut(s) 1100
Bsp120I GGGCCC 2 cut(s) 2847, 4501
Bsp1407I TGTACA 1 cut(s) 4138
Bsp1720I GCTNAGC 1 cut(s) 4326
Bsp19I CCATGG 1 cut(s) 1316
BspACI CCGC 9 cut(s) 163, 1043, 2104, 2615, 2629, 3071, 3519, 4381, 4632
BspHI TCATGA 6 cut(s) 85, 151, 1123, 1360, 2275, 2905
BspMI ACCTGC 1 cut(s) 3628
BspQI GCTCTTC 2 cut(s) 3213, 3854
BspT104I TTCGAA 1 cut(s) 1100
BspT107I GGYRCC 4 cut(s) 1258, 2491, 2813, 4388
BsrDI GCAATG 4 cut(s) 2939, 3253, 4390, 4740
BsrFI RCCGGY 2 cut(s) 2755, 4620
BsrGI TGTACA 1 cut(s) 4138
BsrI ACTGG 9 cut(s) 145, 989, 1454, 1506, 2738, 2945, 3560, 4082, 4677
BssAI RCCGGY 2 cut(s) 2755, 4620
BssNAI GTATAC 1 cut(s) 790
BssT1I CCWWGG 6 cut(s) 521, 1316, 1904, 3342, 3743, 3900
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 8 cut(s) 666, 1791, 2489, 3150, 3365, 3627, 3834, 4562
Bst4CI ACNGT 7 cut(s) 686, 1354, 2381, 2661, 2916, 3037, 3097
Bst6I CTCTTC 7 cut(s) 541, 2422, 3213, 3397, 3540, 3854, 4304
BstAPI GCANNNNNTGC 3 cut(s) 299, 3107, 4226
BstAUI TGTACA 1 cut(s) 4138
BstBAI YACGTR 2 cut(s) 3293, 4333
BstBI TTCGAA 1 cut(s) 1100
BstC8I GCNNGC 8 cut(s) 161, 452, 1047, 1283, 3255, 3751, 4558, 4622
BstDSI CCRYGG 3 cut(s) 613, 649, 1316
BstENI CCTNNNNNAGG 2 cut(s) 1831, 2808
BstHHI GCGC 2 cut(s) 198, 4769
BstMAI GTCTC 6 cut(s) 819, 1420, 2712, 3086, 3800, 3954
BstNI CCWGG 8 cut(s) 666, 1791, 2489, 3150, 3365, 3627, 3834, 4562
BstNSI RCATGY 5 cut(s) 454, 1285, 2536, 3182, 3599
BstPAI GACNNNNGTC 1 cut(s) 2104
BstSFI CTRYAG 2 cut(s) 708, 2252
BstSLI GKGCMC 8 cut(s) 304, 895, 961, 1720, 1804, 2851, 4393, 4505
BstV2I GAAGAC 3 cut(s) 1041, 1190, 3114
BstX2I RGATCY 7 cut(s) 1435, 1513, 1615, 1693, 2245, 2434, 2701
BstXI CCANNNNNNTGG 3 cut(s) 2086, 3037, 3515
BstYI RGATCY 7 cut(s) 1435, 1513, 1615, 1693, 2245, 2434, 2701
BstZ17I GTATAC 1 cut(s) 790
BsuI GTATCC 1 cut(s) 1816
BtgI CCRYGG 3 cut(s) 613, 649, 1316
BtrI CACGTC 2 cut(s) 296, 1416
BtsI GCAGTG 1 cut(s) 3582
BtsIMutI CAGTG 8 cut(s) 691, 1513, 1955, 2952, 3582, 4110, 4248, 4670
BveI ACCTGC 1 cut(s) 3628
Cac8I GCNNGC 8 cut(s) 161, 452, 1047, 1283, 3255, 3751, 4558, 4622
CaiI CAGNNNCTG 4 cut(s) 1934, 3014, 3107, 3977
CciI TCATGA 6 cut(s) 85, 151, 1123, 1360, 2275, 2905
CfoI GCGC 2 cut(s) 198, 4769
Cfr10I RCCGGY 2 cut(s) 2755, 4620
CseI GACGC 1 cut(s) 1499
DraI TTTAAA 1 cut(s) 3117
DraIII CACNNNGTG 2 cut(s) 299, 1748
EaeI YGGCCR 4 cut(s) 646, 1464, 2076, 3759
Eam1104I CTCTTC 7 cut(s) 541, 2422, 3213, 3397, 3540, 3854, 4304
EarI CTCTTC 7 cut(s) 541, 2422, 3213, 3397, 3540, 3854, 4304
Ecl136II GAGCTC 2 cut(s) 1116, 2779
Eco130I CCWWGG 6 cut(s) 521, 1316, 1904, 3342, 3743, 3900
Eco24I GRGCYC 6 cut(s) 1118, 1327, 2781, 2851, 3866, 4505
Eco32I GATATC 1 cut(s) 4291
Eco47I GGWCC 6 cut(s) 318, 386, 1235, 2912, 2985, 4245
Eco53kI GAGCTC 2 cut(s) 1116, 2779
Eco57I CTGAAG 8 cut(s) 1233, 2028, 2699, 3291, 3306, 3708, 3723, 4587
Eco72I CACGTG 1 cut(s) 4333
EcoICRI GAGCTC 2 cut(s) 1116, 2779
EcoNI CCTNNNNNAGG 2 cut(s) 1831, 2808
EcoO109I RGGNCCY 8 cut(s) 386, 1235, 1637, 2847, 2848, 2985, 4178, 4501
EcoRII CCWGG 8 cut(s) 664, 1789, 2487, 3148, 3363, 3625, 3832, 4560
EcoRV GATATC 1 cut(s) 4291
EcoT14I CCWWGG 6 cut(s) 521, 1316, 1904, 3342, 3743, 3900
EcoT22I ATGCAT 1 cut(s) 2695
EcoT38I GRGCYC 6 cut(s) 1118, 1327, 2781, 2851, 3866, 4505
ErhI CCWWGG 6 cut(s) 521, 1316, 1904, 3342, 3743, 3900
Esp3I CGTCTC 3 cut(s) 819, 2712, 3954
FalI AAGNNNNNCTT 2 cut(s) 4779, 4811
FaqI GGGAC 7 cut(s) 191, 233, 1238, 2925, 2998, 3692, 4795
FauI CCCGC 4 cut(s) 156, 2636, 3512, 4388
FbaI TGATCA 1 cut(s) 2278
FblI GTMKAC 1 cut(s) 789
FriOI GRGCYC 6 cut(s) 1118, 1327, 2781, 2851, 3866, 4505
FspBI CTAG 7 cut(s) 603, 1263, 1547, 1779, 2388, 3906, 4770
FspI TGCGCA 1 cut(s) 197
GlaI GCGC 2 cut(s) 197, 4768
GsaI CCCAGC 1 cut(s) 4366
GsuI CTGGAG 2 cut(s) 1560, 4221
HapII CCGG 6 cut(s) 547, 581, 1286, 1677, 2756, 4621
HgaI GACGC 1 cut(s) 1499
HhaI GCGC 2 cut(s) 198, 4769
Hin6I GCGC 2 cut(s) 196, 4767
HinP1I GCGC 2 cut(s) 196, 4767
HincII GTYRAC 2 cut(s) 589, 2377
HindII GTYRAC 2 cut(s) 589, 2377
HindIII AAGCTT 3 cut(s) 673, 1019, 4262
HpaII CCGG 6 cut(s) 547, 581, 1286, 1677, 2756, 4621
HphI GGTGA 8 cut(s) 86, 1578, 1722, 1760, 2961, 3277, 3470, 4069
Hpy99I CGWCG 2 cut(s) 258, 300
HpyCH4III ACNGT 7 cut(s) 686, 1354, 2381, 2661, 2916, 3037, 3097
HpyCH4IV ACGT 4 cut(s) 295, 1415, 3292, 4332
HpySE526I ACGT 4 cut(s) 295, 1415, 3292, 4332
HspAI GCGC 2 cut(s) 196, 4767
KflI GGGWCCC 1 cut(s) 2985
KroI GCCGGC 1 cut(s) 4620
KroNI GCCGGC 1 cut(s) 4622
Ksp22I TGATCA 1 cut(s) 2278
LguI GCTCTTC 2 cut(s) 3213, 3854
LweI GCATC 7 cut(s) 2702, 2729, 3058, 3119, 3514, 3790, 4699
MaeI CTAG 7 cut(s) 603, 1263, 1547, 1779, 2388, 3906, 4770
MaeII ACGT 4 cut(s) 295, 1415, 3292, 4332
MflI RGATCY 7 cut(s) 1435, 1513, 1615, 1693, 2245, 2434, 2701
MlsI TGGCCA 2 cut(s) 2078, 3761
MluNI TGGCCA 2 cut(s) 2078, 3761
MlyI GAGTC 7 cut(s) 317, 347, 425, 571, 1961, 4030, 4247
MmeI TCCRAC 8 cut(s) 236, 457, 656, 1624, 2008, 2338, 3580, 3593
Mox20I TGGCCA 2 cut(s) 2078, 3761
Mph1103I ATGCAT 1 cut(s) 2695
MroNI GCCGGC 1 cut(s) 4620
MroXI GAANNNNTTC 4 cut(s) 1202, 2035, 2733, 3534
MscI TGGCCA 2 cut(s) 2078, 3761
MslI CAYNNNNRTG 1 cut(s) 3183
Msp20I TGGCCA 2 cut(s) 2078, 3761
MspA1I CMGCKG 3 cut(s) 1045, 2104, 3226
MspI CCGG 6 cut(s) 547, 581, 1286, 1677, 2756, 4621
Mva1269I GAATGC 3 cut(s) 1303, 2695, 3286
MvaI CCWGG 8 cut(s) 666, 1791, 2489, 3150, 3365, 3627, 3834, 4562
NaeI GCCGGC 1 cut(s) 4622
NciI CCSGG 2 cut(s) 548, 1287
NcoI CCATGG 1 cut(s) 1316
NgoMIV GCCGGC 1 cut(s) 4620
NmeAIII GCCGAG 4 cut(s) 464, 1276, 2689, 2986
NmuCI GTSAC 8 cut(s) 416, 844, 1419, 1953, 3265, 3458, 4075, 4401
NsbI TGCGCA 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 2695
NspI RCATGY 5 cut(s) 454, 1285, 2536, 3182, 3599
NspV TTCGAA 1 cut(s) 1100
PaeI GCATGC 2 cut(s) 454, 1285
PagI TCATGA 6 cut(s) 85, 151, 1123, 1360, 2275, 2905
PciI ACATGT 2 cut(s) 2532, 3595
PciSI GCTCTTC 2 cut(s) 3213, 3854
PctI GAATGC 3 cut(s) 1303, 2695, 3286
PdiI GCCGGC 1 cut(s) 4622
PdmI GAANNNNTTC 4 cut(s) 1202, 2035, 2733, 3534
PflFI GACNNNGTC 1 cut(s) 1417
PflMI CCANNNNNTGG 4 cut(s) 35, 1790, 1844, 2654
PfoI TCCNGGA 1 cut(s) 1789
PleI GAGTC 7 cut(s) 317, 346, 425, 571, 1960, 4029, 4247
PmaCI CACGTG 1 cut(s) 4333
PmlI CACGTG 1 cut(s) 4333
PpsI GAGTC 7 cut(s) 317, 346, 425, 571, 1960, 4029, 4247
Ppu21I YACGTR 2 cut(s) 3293, 4333
PpuMI RGGWCCY 3 cut(s) 386, 1235, 2985
PscI ACATGT 2 cut(s) 2532, 3595
PshAI GACNNNNGTC 1 cut(s) 2104
PsiI TTATAA 2 cut(s) 1611, 4476
Psp124BI GAGCTC 2 cut(s) 1118, 2781
Psp5II RGGWCCY 3 cut(s) 386, 1235, 2985
Psp6I CCWGG 8 cut(s) 664, 1789, 2487, 3148, 3363, 3625, 3832, 4560
PspCI CACGTG 1 cut(s) 4333
PspFI CCCAGC 1 cut(s) 4362
PspGI CCWGG 8 cut(s) 664, 1789, 2487, 3148, 3363, 3625, 3832, 4560
PspOMI GGGCCC 2 cut(s) 2847, 4501
PspPPI RGGWCCY 3 cut(s) 386, 1235, 2985
PsrI GAACNNNNNNTAC 2 cut(s) 1119, 1151
PstNI CAGNNNCTG 4 cut(s) 1934, 3014, 3107, 3977
PsuI RGATCY 7 cut(s) 1435, 1513, 1615, 1693, 2245, 2434, 2701
PsyI GACNNNGTC 1 cut(s) 1417
PvuII CAGCTG 1 cut(s) 3226
RseI CAYNNNNRTG 1 cut(s) 3183
SacI GAGCTC 2 cut(s) 1118, 2781
SapI GCTCTTC 2 cut(s) 3213, 3854
ScaI AGTACT 3 cut(s) 2468, 4158, 4585
SchI GAGTC 7 cut(s) 317, 347, 425, 571, 1961, 4030, 4247
SfaNI GCATC 7 cut(s) 2702, 2729, 3058, 3119, 3514, 3790, 4699
SfcI CTRYAG 2 cut(s) 708, 2252
SfuI TTCGAA 1 cut(s) 1100
SinI GGWCC 6 cut(s) 318, 386, 1235, 2912, 2985, 4245
SmiMI CAYNNNNRTG 1 cut(s) 3183
SmlI CTYRAG 6 cut(s) 325, 963, 1828, 2367, 2808, 2978
SmoI CTYRAG 6 cut(s) 325, 963, 1828, 2367, 2808, 2978
SphI GCATGC 2 cut(s) 454, 1285
SsiI CCGC 9 cut(s) 163, 1043, 2104, 2615, 2629, 3071, 3519, 4381, 4632
SspI AATATT 3 cut(s) 2875, 2956, 3780
SspMI CTAG 7 cut(s) 603, 1263, 1547, 1779, 2388, 3906, 4770
SstI GAGCTC 2 cut(s) 1118, 2781
StyI CCWWGG 6 cut(s) 521, 1316, 1904, 3342, 3743, 3900
TaaI ACNGT 7 cut(s) 686, 1354, 2381, 2661, 2916, 3037, 3097
TaiI ACGT 4 cut(s) 298, 1418, 3295, 4335
TaqI TCGA 3 cut(s) 817, 1100, 1151
TaqII GACCGA 1 cut(s) 335
TatI WGTACW 6 cut(s) 2466, 2529, 3592, 4138, 4156, 4583
TauI GCSGC 1 cut(s) 3074
TscAI CASTG 8 cut(s) 691, 1513, 1962, 2952, 3582, 4117, 4255, 4677
TseFI GTSAC 8 cut(s) 416, 844, 1419, 1953, 3265, 3458, 4075, 4401
Tsp45I GTSAC 8 cut(s) 416, 844, 1419, 1953, 3265, 3458, 4075, 4401
TspGWI ACGGA 3 cut(s) 501, 2109, 2694
TspRI CASTG 8 cut(s) 691, 1513, 1962, 2952, 3582, 4117, 4255, 4677
Tth111I GACNNNGTC 1 cut(s) 1417
Van91I CCANNNNNTGG 4 cut(s) 35, 1790, 1844, 2654
VneI GTGCAC 5 cut(s) 300, 891, 957, 1716, 1800
VpaK11BI GGWCC 6 cut(s) 318, 386, 1235, 2912, 2985, 4245
XagI CCTNNNNNAGG 2 cut(s) 1831, 2808
XapI RAATTY 6 cut(s) 714, 898, 2635, 3435, 3654, 3933
XbaI TCTAGA 1 cut(s) 1546
XceI RCATGY 5 cut(s) 454, 1285, 2536, 3182, 3599
XcmI CCANNNNNNNNNTGG 2 cut(s) 312, 3596
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 4 cut(s) 1202, 2035, 2733, 3534
XspI CTAG 7 cut(s) 603, 1263, 1547, 1779, 2388, 3906, 4770
ZrmI AGTACT 3 cut(s) 2468, 4158, 4585
Zsp2I ATGCAT 1 cut(s) 2695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.