Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012766

Physical Location & Seq

Genomic Coordinates Reverse (-)

17269310 .. 17272298

Gene Structure 2989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G43060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1392 bp

ATGGGTTTCTTAAAGTTATCTCCAATGATCCTTCTCCTAGCAATGATCGGCGTTTCATATGCCATGGACATGTCAATCATCTCCTACGACGAAAACCACCACATCACCACCGAGACCAGCCGTAGCGACTCGGAGGTTGAGAGAATCTACGAAGCGTGGATGGTGGAACACGGGAAGAAGAAGATGAATCAAAACGGTCTTGGTGCAGAGAAAGATCAAAGATTCGAGATCTTTAAAGATAATCTTCGTTTCATCGATGAACATAACACCAAAAACCTCAGTTATAAACTTGGTTTAACCCGGTTTGCTGATTTAACCAATGAGGAGTACCGATCTATGTACCTTGGTGCTAAACCGACCAAGAGGGTTTTGAAAACCAGTGATCGGTATCAGGCGCGTGTTGGTGACGCGCTTCCTGATTCGGTTGACTGGAGAAAAGAAGGTGCCGTCGCTGATGTTAAAGATCAAGGAAGCTGTGGGAGTTGTTGGGCATTCTCAACTATTGGAGCAGTGGAAGGAATAAACAAGATTGTGACAGGAGATTTAATATCTTTGTCTGAACAAGAATTGGTTGATTGTGACACATCTTATAACCAAGGATGTAATGGTGGTTTAATGGACTATGCGTTTGAGTTTATTATCAAAAATGGTGGTATTGATACCGAAGCGGATTATCCTTACAAGGCTGCTGATGGTCGTTGTGACCAGAACAGGAAAAATGCTAAGGTTGTTACCATTGATTCATATGAAGATGTCCCTGAGAACAGCGAGGCGTCTCTAAAGAAAGCTTTGGCTCACCAGCCTATTAGTGTCGCCATTGAAGCGGGTGGTCGCGCTTTCCAGCTCTATTCTTCGGGTGTATTTGATGGACTTTGTGGGACGGAGTTAGACCACGGTGTTGTGGCTGTGGGTTATGGAACCGAGAACGGAAAGGACTACTGGATTGTGAGAAACTCATGGGGAAACAGATGGGGAGAGAGCGGATACATAAAGATGGCACGTAACATTGAAGCGCCAACAGGAAAGTGTGGAATCGCTATGGAGGCTTCGTATCCGATCAAGAAAGGTCAGAACCCACCAAACCCTGGCCCGTCACCACCATCTCCCATTAAGCCACCAACCACATGTGACAAGTACTTCTCTTGCCCTGAGAGCAACACTTGTTGTTGTCTCTACAAGTATGGTAAGTACTGCTTCGGATGGGGATGTTGCCCGCTCGAAGCTGCTACTTGCTGCGATGATAACTCAAGTTGCTGTCCTCATGAGTACCCGGTTTGTGACGTTAACCGCGGAACCTGTTTGATGAGCAAGAACAGTCCGTTTAGCGTGAAGGCTTTAAAGCGTACACCCGCAATACCGTTCTGGGCAAAGAGCAGAAAGCACATTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009987 GO:0010494 GO:0012505 GO:0016787 GO:0019538 GO:0030054 GO:0030163 GO:0030312 GO:0032991 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0070011 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

463

Amino Acids

51.2

Weight (kDa)

5.86

Isoelectric Point (pI)

39.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	50 - 110	2.1e-16	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	138 - 353	1.3e-88	Papain family cysteine protease
Granulin	PF00396	387 - 434	6.3e-09	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47128 AT5G43060
fragaria_vesca	FvH4_2g33910
malus_domestica	MD08G1065500.v1.1 MD08G1093900.v1.1 MD15G1078300.v1.1
prunus_persica	Prupe.1G431000_v2.0.a1
pyrus_communis	pycom08g07660 pycom15g07310
rosa_chinensis	RchiOBHm_Chr6g0308501
rosa_laevigata	RLG00000010637
rosa_multiflora	Rmu_sc0020439.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160070
rosa_rugosa	Rorug06G0367300
rosa_samantha	Rh6AG477200 Rh6BG487400 Rh6DG478500
rosa_wichuraiana	Rw6G041640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	285, 591
AccB1I	GGYRCC	1 cut(s)	443
AccB7I	CCANNNNNTGG	1 cut(s)	1085
AccBSI	CCGCTC	2 cut(s)	981, 1216
AccII	CGCG	4 cut(s)	397, 410, 834, 1290
AciI	CCGC	7 cut(s)	668, 824, 981, 1214, 1288, 1290, 1350
AclWI	GGATC	1 cut(s)	22
AcyI	GRCGYC	1 cut(s)	773
AfaI	GTAC	6 cut(s)	329, 341, 1136, 1190, 1268, 1345
AfiI	CCNNNNNNNGG	2 cut(s)	384, 1085
AflIII	ACRYGT	2 cut(s)	69, 1124
AgsI	TTSAA	3 cut(s)	373, 821, 1010
AjnI	CCWGG	1 cut(s)	1084
AluBI	AGCT	4 cut(s)	474, 788, 844, 1223
AluI	AGCT	4 cut(s)	474, 788, 844, 1223
Alw26I	GTCTC	3 cut(s)	107, 780, 1175
AlwI	GGATC	1 cut(s)	22
AoxI	GGCC	1 cut(s)	1087
ApeKI	GCWGC	3 cut(s)	686, 1223, 1233
ArsI	GACNNNNNNTTYG	2 cut(s)	611, 643
AspLEI	GCGC	4 cut(s)	397, 412, 836, 1015
AspS9I	GGNCC	1 cut(s)	1088
AsuC2I	CCSGG	2 cut(s)	301, 1271
AsuHPI	GGTGA	4 cut(s)	97, 416, 788, 1086
BanI	GGYRCC	1 cut(s)	443
BbvI	GCAGC	3 cut(s)	673, 1210, 1220
BccI	CCATC	7 cut(s)	154, 686, 860, 963, 988, 1108, 1194
BceAI	ACGGC	2 cut(s)	105, 431
BciT130I	CCWGG	1 cut(s)	1086
BciVI	GTATCC	2 cut(s)	977, 1062
BcnI	CCSGG	2 cut(s)	301, 1271
BcoDI	GTCTC	3 cut(s)	107, 780, 1175
BfaI	CTAG	1 cut(s)	38
BfoI	RGCGCY	1 cut(s)	1016
BfuI	GTATCC	2 cut(s)	977, 1062
BglII	AGATCT	1 cut(s)	228
BisI	GCNGC	3 cut(s)	687, 1224, 1234
BlsI	GCNGC	3 cut(s)	688, 1225, 1235
BmcAI	AGTACT	2 cut(s)	1136, 1190
Bme1390I	CCNGG	3 cut(s)	301, 1086, 1271
BmgT120I	GGNCC	1 cut(s)	1088
BmiI	GGNNCC	3 cut(s)	445, 919, 1294
BmrFI	CCNGG	3 cut(s)	301, 1086, 1271
BpmI	CTGGAG	1 cut(s)	451
Bpu10I	CCTNAGC	1 cut(s)	723
BpuEI	CTTGAG	1 cut(s)	1231
BpuMI	CCSGG	2 cut(s)	301, 1271
Bsa29I	ATCGAT	1 cut(s)	255
BsaAI	YACGTR	1 cut(s)	1001
BsaBI	GATNNNNATC	1 cut(s)	387
BsaHI	GRCGYC	1 cut(s)	773
BsaI	GGTCTC	1 cut(s)	107
BsaJI	CCNNGG	6 cut(s)	63, 343, 595, 892, 1084, 1288
BsaXI	ACNNNNNCTCC	2 cut(s)	1034, 1064
Bsc4I	CCNNNNNNNGG	2 cut(s)	384, 1085
Bse1I	ACTGG	3 cut(s)	378, 434, 944
Bse3DI	GCAATG	2 cut(s)	48, 1383
Bse8I	GATNNNNATC	1 cut(s)	387
BseBI	CCWGG	1 cut(s)	1086
BseCI	ATCGAT	1 cut(s)	255
BseDI	CCNNGG	6 cut(s)	63, 343, 595, 892, 1084, 1288
BseGI	GGATG	4 cut(s)	165, 605, 1205, 1211
BseJI	GATNNNNATC	1 cut(s)	387
BseLI	CCNNNNNNNGG	2 cut(s)	384, 1085
BseMI	GCAATG	2 cut(s)	48, 1383
BseMII	CTCAG	3 cut(s)	292, 750, 1140
BseNI	ACTGG	3 cut(s)	378, 434, 944
BseRI	GAGGAG	1 cut(s)	338
BseXI	GCAGC	3 cut(s)	673, 1210, 1220
BsgI	GTGCAG	1 cut(s)	225
Bsh1236I	CGCG	4 cut(s)	397, 410, 834, 1290
BshFI	GGCC	1 cut(s)	1089
BshNI	GGYRCC	1 cut(s)	443
BshVI	ATCGAT	1 cut(s)	255
BsiSI	CCGG	2 cut(s)	301, 1271
BslFI	GGGAC	2 cut(s)	740, 892
BslI	CCNNNNNNNGG	2 cut(s)	384, 1085
BsmAI	GTCTC	3 cut(s)	107, 780, 1175
BsmBI	CGTCTC	1 cut(s)	780
BsmFI	GGGAC	2 cut(s)	740, 892
BsmI	GAATGC	1 cut(s)	491
BsnI	GGCC	1 cut(s)	1089
Bso31I	GGTCTC	1 cut(s)	107
Bsp143I	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
Bsp19I	CCATGG	1 cut(s)	63
BspACI	CCGC	7 cut(s)	668, 824, 981, 1214, 1288, 1290, 1350
BspANI	GGCC	1 cut(s)	1089
BspCNI	CTCAG	3 cut(s)	291, 751, 1141
BspDI	ATCGAT	1 cut(s)	255
BspFNI	CGCG	4 cut(s)	397, 410, 834, 1290
BspHI	TCATGA	1 cut(s)	1261
BspLI	GGNNCC	3 cut(s)	445, 919, 1294
BspPI	GGATC	1 cut(s)	22
BspT107I	GGYRCC	1 cut(s)	443
BspTNI	GGTCTC	1 cut(s)	107
BsrBI	CCGCTC	2 cut(s)	981, 1216
BsrDI	GCAATG	2 cut(s)	48, 1383
BsrI	ACTGG	3 cut(s)	378, 434, 944
BssECI	CCNNGG	6 cut(s)	63, 343, 595, 892, 1084, 1288
BssMI	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
BssNI	GRCGYC	1 cut(s)	773
BssT1I	CCWWGG	3 cut(s)	63, 343, 595
Bst2UI	CCWGG	1 cut(s)	1086
Bst4CI	ACNGT	4 cut(s)	197, 896, 1316, 1359
BstACI	GRCGYC	1 cut(s)	773
BstBAI	YACGTR	1 cut(s)	1001
BstC8I	GCNNGC	1 cut(s)	1214
BstDEI	CTNAG	4 cut(s)	278, 723, 759, 1149
BstDSI	CCRYGG	3 cut(s)	63, 892, 1288
BstF5I	GGATG	4 cut(s)	165, 605, 1205, 1211
BstFNI	CGCG	4 cut(s)	397, 410, 834, 1290
BstH2I	RGCGCY	1 cut(s)	1016
BstHHI	GCGC	4 cut(s)	397, 412, 836, 1015
BstKTI	GATC	8 cut(s)	30, 48, 217, 231, 335, 385, 466, 1059
BstMAI	GTCTC	3 cut(s)	107, 780, 1175
BstMBI	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
BstMWI	GCNNNNNNNGC	3 cut(s)	821, 1043, 1152
BstNI	CCWGG	1 cut(s)	1086
BstNSI	RCATGY	2 cut(s)	73, 1128
BstSCI	CCNGG	3 cut(s)	299, 1084, 1269
BstUI	CGCG	4 cut(s)	397, 410, 834, 1290
BstV1I	GCAGC	3 cut(s)	673, 1210, 1220
BstX2I	RGATCY	1 cut(s)	228
BstYI	RGATCY	1 cut(s)	228
Bsu15I	ATCGAT	1 cut(s)	255
BsuI	GTATCC	2 cut(s)	977, 1062
BsuRI	GGCC	1 cut(s)	1089
BsuTUI	ATCGAT	1 cut(s)	255
BtgI	CCRYGG	3 cut(s)	63, 892, 1288
BtgZI	GCGATG	1 cut(s)	1251
BtsCI	GGATG	4 cut(s)	165, 605, 1205, 1211
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	2 cut(s)	385, 516
Cac8I	GCNNGC	1 cut(s)	1214
CciI	TCATGA	1 cut(s)	1261
CfoI	GCGC	4 cut(s)	397, 412, 836, 1015
Cfr13I	GGNCC	1 cut(s)	1088
Cfr42I	CCGCGG	1 cut(s)	1291
ClaI	ATCGAT	1 cut(s)	255
CseI	GACGC	2 cut(s)	416, 762
Csp6I	GTAC	6 cut(s)	328, 340, 1135, 1189, 1267, 1344
CspCI	CAANNNNNGTGG	2 cut(s)	631, 666
CviAII	CATG	5 cut(s)	64, 70, 957, 1125, 1262
CviQI	GTAC	6 cut(s)	328, 340, 1135, 1189, 1267, 1344
DdeI	CTNAG	4 cut(s)	278, 723, 759, 1149
DpnI	GATC	8 cut(s)	29, 47, 216, 230, 334, 384, 465, 1058
DpnII	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
DraI	TTTAAA	2 cut(s)	235, 1338
Eco130I	CCWWGG	3 cut(s)	63, 343, 595
Eco31I	GGTCTC	1 cut(s)	107
EcoRII	CCWGG	1 cut(s)	1084
EcoT14I	CCWWGG	3 cut(s)	63, 343, 595
ErhI	CCWWGG	3 cut(s)	63, 343, 595
Esp3I	CGTCTC	1 cut(s)	780
FaeI	CATG	5 cut(s)	67, 73, 960, 1128, 1265
FalI	AAGNNNNNCTT	4 cut(s)	228, 260, 1178, 1210
FaqI	GGGAC	2 cut(s)	740, 892
FatI	CATG	5 cut(s)	63, 69, 956, 1124, 1261
FauI	CCCGC	3 cut(s)	817, 1221, 1357
FauNDI	CATATG	2 cut(s)	58, 745
Fnu4HI	GCNGC	3 cut(s)	687, 1224, 1234
FokI	GGATG	4 cut(s)	172, 612, 1212, 1218
Fsp4HI	GCNGC	3 cut(s)	687, 1224, 1234
FspBI	CTAG	1 cut(s)	38
GlaI	GCGC	4 cut(s)	396, 411, 835, 1014
GluI	GCNGC	3 cut(s)	687, 1224, 1234
GsuI	CTGGAG	1 cut(s)	451
HaeII	RGCGCY	1 cut(s)	1016
HaeIII	GGCC	1 cut(s)	1089
HapII	CCGG	2 cut(s)	301, 1271
HgaI	GACGC	2 cut(s)	416, 762
HhaI	GCGC	4 cut(s)	397, 412, 836, 1015
Hin1I	GRCGYC	1 cut(s)	773
Hin1II	CATG	5 cut(s)	67, 73, 960, 1128, 1265
Hin6I	GCGC	4 cut(s)	395, 410, 834, 1013
HinP1I	GCGC	4 cut(s)	395, 410, 834, 1013
HincII	GTYRAC	2 cut(s)	427, 1285
HindII	GTYRAC	2 cut(s)	427, 1285
HindIII	AAGCTT	1 cut(s)	786
HinfI	GANTC	7 cut(s)	128, 144, 187, 222, 419, 740, 1032
HpaI	GTTAAC	1 cut(s)	1285
HpaII	CCGG	2 cut(s)	301, 1271
HphI	GGTGA	4 cut(s)	97, 416, 788, 1086
Hpy166II	GTNNAC	3 cut(s)	427, 1285, 1346
Hpy188I	TCNGA	5 cut(s)	133, 559, 1056, 1071, 1199
Hpy188III	TCNNGA	4 cut(s)	226, 416, 1060, 1262
Hpy8I	GTNNAC	3 cut(s)	427, 1285, 1346
Hpy99I	CGWCG	2 cut(s)	92, 452
HpyAV	CCTTC	4 cut(s)	41, 434, 509, 1324
HpyCH4III	ACNGT	4 cut(s)	197, 896, 1316, 1359
HpyCH4IV	ACGT	2 cut(s)	1000, 1281
HpyCH4V	TGCA	1 cut(s)	206
HpyF10VI	GCNNNNNNNGC	3 cut(s)	821, 1043, 1152
HpyF3I	CTNAG	4 cut(s)	278, 723, 759, 1149
HpySE526I	ACGT	2 cut(s)	1000, 1281
Hsp92I	GRCGYC	1 cut(s)	773
Hsp92II	CATG	5 cut(s)	67, 73, 960, 1128, 1265
HspAI	GCGC	4 cut(s)	395, 410, 834, 1013
KspAI	GTTAAC	1 cut(s)	1285
KspI	CCGCGG	1 cut(s)	1291
Kzo9I	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
LmnI	GCTCC	1 cut(s)	506
Lsp1109I	GCAGC	3 cut(s)	673, 1210, 1220
MaeI	CTAG	1 cut(s)	38
MaeII	ACGT	2 cut(s)	1000, 1281
MaeIII	GTNAC	9 cut(s)	404, 532, 578, 701, 730, 1001, 1092, 1127, 1277
MalI	GATC	8 cut(s)	29, 47, 216, 230, 334, 384, 465, 1058
MbiI	CCGCTC	2 cut(s)	981, 1216
MboI	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
MboII	GAAGA	6 cut(s)	187, 190, 193, 236, 761, 843
MflI	RGATCY	1 cut(s)	228
MluCI	AATT	1 cut(s)	566
MlyI	GAGTC	1 cut(s)	122
MnlI	CCTC	7 cut(s)	127, 287, 316, 357, 763, 1036, 1269
MslI	CAYNNNNRTG	2 cut(s)	68, 992
MspA1I	CMGCKG	1 cut(s)	1290
MspI	CCGG	2 cut(s)	301, 1271
MspR9I	CCNGG	3 cut(s)	301, 1086, 1271
Mva1269I	GAATGC	1 cut(s)	491
MvaI	CCWGG	1 cut(s)	1086
MvnI	CGCG	4 cut(s)	397, 410, 834, 1290
MwoI	GCNNNNNNNGC	3 cut(s)	821, 1043, 1152
NciI	CCSGG	2 cut(s)	301, 1271
NcoI	CCATGG	1 cut(s)	63
NdeI	CATATG	2 cut(s)	58, 745
NdeII	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
NlaIII	CATG	5 cut(s)	67, 73, 960, 1128, 1265
NlaIV	GGNNCC	3 cut(s)	445, 919, 1294
NmuCI	GTSAC	7 cut(s)	404, 532, 578, 701, 1092, 1127, 1277
NspI	RCATGY	2 cut(s)	73, 1128
PagI	TCATGA	1 cut(s)	1261
PciI	ACATGT	2 cut(s)	69, 1124
PctI	GAATGC	1 cut(s)	491
PfeI	GAWTC	6 cut(s)	144, 187, 222, 419, 740, 1032
PflMI	CCANNNNNTGG	1 cut(s)	1085
PkrI	GCNGC	3 cut(s)	688, 1225, 1235
PleI	GAGTC	1 cut(s)	122
PpsI	GAGTC	1 cut(s)	122
Ppu21I	YACGTR	1 cut(s)	1001
PscI	ACATGT	2 cut(s)	69, 1124
PsiI	TTATAA	2 cut(s)	285, 591
Psp6I	CCWGG	1 cut(s)	1084
PspGI	CCWGG	1 cut(s)	1084
PspN4I	GGNNCC	3 cut(s)	445, 919, 1294
PspPI	GGNCC	1 cut(s)	1088
PsuI	RGATCY	1 cut(s)	228
RsaI	GTAC	6 cut(s)	329, 341, 1136, 1190, 1268, 1345
RsaNI	GTAC	6 cut(s)	328, 340, 1135, 1189, 1267, 1344
RseI	CAYNNNNRTG	2 cut(s)	68, 992
SacII	CCGCGG	1 cut(s)	1291
SatI	GCNGC	3 cut(s)	687, 1224, 1234
Sau3AI	GATC	8 cut(s)	27, 45, 214, 228, 332, 382, 463, 1056
Sau96I	GGNCC	1 cut(s)	1088
ScaI	AGTACT	2 cut(s)	1136, 1190
SchI	GAGTC	1 cut(s)	122
ScrFI	CCNGG	3 cut(s)	301, 1086, 1271
Sfr303I	CCGCGG	1 cut(s)	1291
SgrBI	CCGCGG	1 cut(s)	1291
SmiMI	CAYNNNNRTG	2 cut(s)	68, 992
SmlI	CTYRAG	1 cut(s)	1246
SmoI	CTYRAG	1 cut(s)	1246
Sse9I	AATT	1 cut(s)	566
SsiI	CCGC	7 cut(s)	668, 824, 981, 1214, 1288, 1290, 1350
SspMI	CTAG	1 cut(s)	38
StyD4I	CCNGG	3 cut(s)	299, 1084, 1269
StyI	CCWWGG	3 cut(s)	63, 343, 595
TaaI	ACNGT	4 cut(s)	197, 896, 1316, 1359
TaiI	ACGT	2 cut(s)	1003, 1284
TaqI	TCGA	3 cut(s)	225, 255, 1218
TasI	AATT	1 cut(s)	566
TatI	WGTACW	2 cut(s)	1134, 1188
TfiI	GAWTC	6 cut(s)	144, 187, 222, 419, 740, 1032
TscAI	CASTG	2 cut(s)	385, 516
TseFI	GTSAC	7 cut(s)	404, 532, 578, 701, 1092, 1127, 1277
TseI	GCWGC	3 cut(s)	686, 1223, 1233
Tsp45I	GTSAC	7 cut(s)	404, 532, 578, 701, 1092, 1127, 1277
TspDTI	ATGAA	6 cut(s)	45, 200, 241, 273, 732, 762
TspGWI	ACGGA	3 cut(s)	896, 942, 1308
TspRI	CASTG	2 cut(s)	385, 516
Van91I	CCANNNNNTGG	1 cut(s)	1085
XceI	RCATGY	2 cut(s)	73, 1128
XcmI	CCANNNNNNNNNTGG	1 cut(s)	602
XspI	CTAG	1 cut(s)	38
ZrmI	AGTACT	2 cut(s)	1136, 1190

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.