Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012766

Physical Location & Seq

Genomic Coordinates Reverse (-)

5343564 .. 5346712

Gene Structure 3149 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1078300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGCGGCCGTACCGATCATCGCCGGTCGTGGCGATCCTCCTCCTCACGATCTTCACCGTCTCATCGGCCCTCGACATGTCGATCATCTCCTACGACAGCAAGTCGTCGGGCAGCTGGAGGACAGACGATGAGGTGATGTCGATATACGAGGGGTGGCTGGTGGAGCACGGAAAGGCATACAACGCTTTGGGAGAGAAGGAGAGAAGGTTCCAGATCTTCAAGGACAACCTTAGGTACATCGACGAACAAAACTCCAAGAACCTCAGCTACAAGCTCGGTCTGAACAGGTTCGCCGATCTGTCGAACGAGGAGTACCGGAACACTTACCTCGGCGCCAAGACTCGCGCGCAGATGAAGCGGGTGTCCAACAGGAACACCAAGAGCGACCGGTACGCGCCACGTGTCGGCGATTCGTTGCCCGACTCCGTTGATTGGAGGAAGGAGGGCGCGGTGAGTCCAGTCAAAGACCAAGGCAGCTGCGGGAGTTGCTGGGCATTCTCAACTATCTCCGCCGTGGAAGGTATCAATAAGCTAGTCACCGGAGATCTCATCTCCCTATCTGAGCAGGAGCTGGTAGACTGCGATAGAACGTACAATGAAGGCTGCAATGGAGGTCTTATGGACTATGGTTTCGAGTTCATCATCAACAATGGCGGCATTGATAGCGAAGAGGACTATCCTTACAAAGGATATGATGCCACATGTGACACCTACAGGAAAAATGCCAAGGTTGTTTCGATTGACGATTACGAAGATGTTCCTACATACAATGAGAAAGCACTGCAGAAGGCTGTTGCCAATCAGCCAATTGCCGTTGCCATTGAAGGGGGTGGCAGAGACTTCCAGTTGTATAACTCCGGTGTGTTTACGGGACGTTGTGGGACAGCACTAGACCATGGTGTTACAGTTGTGGGATATGGCACGGACGACGGACTTGATTACTGGATAGTGAGAAACTCATGGGGTGGAAGCTGGGGAGAGGAGGGATACATCAGGATGCAGCGTAATCTGGGCAACACCGCCAATGGGATATGTGGAATTGCCATGGAGCCTTCTTACCCCATCAAGAAAGGCCAGAATCCCCCTAACCCTGGCCCTTCTCCTCCGTCTCCGGTAAAGCCCCCGTCAGTTTGTGACAACCACTACTCCTGCCCTGAGAGCAACACCTGCTGCTGTATCTATGAGTATGCAAACTATTGCTTTGCTTGGGGATGTTGTCCACTCGAAGGAGCCACCTGCTGTGACGACCATTACAGTTGCTGCCCAAGTGACTATCCCGTGTGCGATGTCAATGCCGGAGCTTGTCTGATGAGCAAGGGCAATCCGTTGGGGGTGAAGGCATTGAAGCGCACCCCCGCAAAACCTCACTGGGCCATTGGTGGTGGAAAGAGCAGCAGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010494 GO:0016787 GO:0019538 GO:0030054 GO:0030163 GO:0032991 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0070011 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

468

Amino Acids

51.29

Weight (kDa)

5.15

Isoelectric Point (pI)

40.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	49 - 105	3.7e-18	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	139 - 355	4.4e-84	Papain family cysteine protease
Granulin	PF00396	389 - 436	2e-08	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47128 AT5G43060
fragaria_vesca	FvH4_2g33910
malus_domestica	MD08G1065500.v1.1 MD08G1093900.v1.1 MD15G1078300.v1.1
prunus_persica	Prupe.1G431000_v2.0.a1
pyrus_communis	pycom08g07660 pycom15g07310
rosa_chinensis	RchiOBHm_Chr6g0308501
rosa_laevigata	RLG00000010637
rosa_multiflora	Rmu_sc0020439.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160070
rosa_rugosa	Rorug06G0367300
rosa_samantha	Rh6AG477200 Rh6BG487400 Rh6DG478500
rosa_wichuraiana	Rw6G041640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1175, 1244
Acc36I	ACCTGC	2 cut(s)	1175, 1244
AccB1I	GGYRCC	1 cut(s)	332
AccI	GTMKAC	1 cut(s)	576
AccII	CGCG	4 cut(s)	345, 347, 395, 449
AciI	CCGC	8 cut(s)	4, 358, 449, 480, 510, 654, 1020, 1356
AclWI	GGATC	1 cut(s)	28
AcoI	YGGCCR	1 cut(s)	5
AcvI	CACGTG	1 cut(s)	401
AcyI	GRCGYC	1 cut(s)	333
AfaI	GTAC	5 cut(s)	11, 236, 314, 392, 593
AfiI	CCNNNNNNNGG	7 cut(s)	404, 432, 686, 825, 1026, 1091, 1226
AflIII	ACRYGT	3 cut(s)	75, 400, 701
AgeI	ACCGGT	1 cut(s)	387
AgsI	TTSAA	3 cut(s)	220, 824, 1345
AhdI	GACNNNNNGTC	1 cut(s)	100
AjnI	CCWGG	1 cut(s)	1090
AloI	GAACNNNNNNTCC	2 cut(s)	191, 223
AluBI	AGCT	8 cut(s)	114, 267, 274, 477, 532, 571, 972, 1301
AluI	AGCT	8 cut(s)	114, 267, 274, 477, 532, 571, 972, 1301
Alw21I	GWGCWC	1 cut(s)	168
Alw26I	GTCTC	3 cut(s)	64, 831, 1113
AlwI	GGATC	1 cut(s)	28
AlwNI	CAGNNNCTG	2 cut(s)	571, 1260
AoxI	GGCC	5 cut(s)	5, 66, 1072, 1093, 1371
ApeKI	GCWGC	8 cut(s)	111, 474, 477, 603, 1000, 1170, 1260, 1392
ArsI	GACNNNNNNTTYG	2 cut(s)	614, 646
AsiGI	ACCGGT	1 cut(s)	387
Asp700I	GAANNNNTTC	2 cut(s)	287, 756
AspLEI	GCGC	6 cut(s)	335, 347, 349, 397, 449, 1350
AspS9I	GGNCC	3 cut(s)	67, 1094, 1371
AsuHPI	GGTGA	5 cut(s)	46, 145, 463, 529, 1345
AxyI	CCTNAGG	1 cut(s)	230
BanI	GGYRCC	1 cut(s)	332
BbrPI	CACGTG	1 cut(s)	401
Bbv12I	GWGCWC	1 cut(s)	168
BbvCI	CCTCAGC	1 cut(s)	263
BbvI	GCAGC	7 cut(s)	123, 464, 486, 590, 1012, 1157, 1247
BccI	CCATC	1 cut(s)	1070
BceAI	ACGGC	2 cut(s)	497, 797
BcgI	CGANNNNNNTGC	2 cut(s)	1274, 1308
BciT130I	CCWGG	1 cut(s)	1092
BciVI	GTATCC	1 cut(s)	980
BcoDI	GTCTC	3 cut(s)	64, 831, 1113
BfaI	CTAG	2 cut(s)	533, 890
BfmI	CTRYAG	2 cut(s)	712, 782
BfoI	RGCGCY	1 cut(s)	336
BfuAI	ACCTGC	2 cut(s)	1175, 1244
BfuI	GTATCC	1 cut(s)	980
BglII	AGATCT	2 cut(s)	213, 544
Bme1390I	CCNGG	1 cut(s)	1092
BmeRI	GACNNNNNGTC	1 cut(s)	100
BmgT120I	GGNCC	3 cut(s)	67, 1094, 1371
BmiI	GGNNCC	4 cut(s)	209, 334, 1050, 1231
BmrFI	CCNGG	1 cut(s)	1092
BmrI	ACTGGG	1 cut(s)	1378
BmsI	GCATC	2 cut(s)	685, 987
BmuI	ACTGGG	1 cut(s)	1378
BpmI	CTGGAG	1 cut(s)	136
Bpu10I	CCTNAGC	1 cut(s)	263
BsaAI	YACGTR	1 cut(s)	401
BsaHI	GRCGYC	1 cut(s)	333
BsaJI	CCNNGG	7 cut(s)	328, 469, 513, 726, 895, 1044, 1090
BsaWI	WCCGGW	5 cut(s)	315, 387, 539, 857, 1111
BsaXI	ACNNNNNCTCC	4 cut(s)	191, 221, 1130, 1160
Bsc4I	CCNNNNNNNGG	7 cut(s)	404, 432, 686, 825, 1026, 1091, 1226
Bse118I	RCCGGY	2 cut(s)	22, 387
Bse1I	ACTGG	4 cut(s)	458, 844, 947, 1373
Bse21I	CCTNAGG	1 cut(s)	230
Bse3DI	GCAATG	1 cut(s)	613
BseBI	CCWGG	1 cut(s)	1092
BseDI	CCNNGG	7 cut(s)	328, 469, 513, 726, 895, 1044, 1090
BseGI	GGATG	2 cut(s)	1002, 1217
BseLI	CCNNNNNNNGG	7 cut(s)	404, 432, 686, 825, 1026, 1091, 1226
BseMI	GCAATG	1 cut(s)	613
BseMII	CTCAG	3 cut(s)	277, 552, 1146
BseNI	ACTGG	4 cut(s)	458, 844, 947, 1373
BsePI	GCGCGC	1 cut(s)	345
BseRI	GAGGAG	5 cut(s)	29, 32, 323, 995, 1092
BseX3I	CGGCCG	1 cut(s)	5
BseXI	GCAGC	7 cut(s)	123, 464, 486, 590, 1012, 1157, 1247
BseYI	CCCAGC	2 cut(s)	489, 972
Bsh1236I	CGCG	4 cut(s)	345, 347, 395, 449
Bsh1285I	CGRYCG	3 cut(s)	8, 27, 388
BshFI	GGCC	5 cut(s)	7, 68, 1074, 1095, 1373
BshNI	GGYRCC	1 cut(s)	332
BshTI	ACCGGT	1 cut(s)	387
BsiEI	CGRYCG	3 cut(s)	8, 27, 388
BsiHKAI	GWGCWC	1 cut(s)	168
BsiSI	CCGG	7 cut(s)	23, 316, 388, 540, 858, 1112, 1296
BslFI	GGGAC	2 cut(s)	885, 895
BslI	CCNNNNNNNGG	7 cut(s)	404, 432, 686, 825, 1026, 1091, 1226
BsmAI	GTCTC	3 cut(s)	64, 831, 1113
BsmBI	CGTCTC	2 cut(s)	64, 1113
BsmFI	GGGAC	2 cut(s)	885, 895
BsmI	GAATGC	1 cut(s)	494
BsnI	GGCC	5 cut(s)	7, 68, 1074, 1095, 1373
Bsp1286I	GDGCHC	1 cut(s)	168
Bsp143I	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
Bsp19I	CCATGG	2 cut(s)	895, 1044
BspACI	CCGC	8 cut(s)	4, 358, 449, 480, 510, 654, 1020, 1356
BspANI	GGCC	5 cut(s)	7, 68, 1074, 1095, 1373
BspCNI	CTCAG	3 cut(s)	276, 553, 1147
BspFNI	CGCG	4 cut(s)	345, 347, 395, 449
BspLI	GGNNCC	4 cut(s)	209, 334, 1050, 1231
BspMAI	CTGCAG	1 cut(s)	786
BspMI	ACCTGC	2 cut(s)	1175, 1244
BspPI	GGATC	1 cut(s)	28
BspT107I	GGYRCC	1 cut(s)	332
BsrDI	GCAATG	1 cut(s)	613
BsrFI	RCCGGY	2 cut(s)	22, 387
BsrI	ACTGG	4 cut(s)	458, 844, 947, 1373
BssAI	RCCGGY	2 cut(s)	22, 387
BssECI	CCNNGG	7 cut(s)	328, 469, 513, 726, 895, 1044, 1090
BssHII	GCGCGC	1 cut(s)	345
BssMI	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
BssNI	GRCGYC	1 cut(s)	333
BssT1I	CCWWGG	4 cut(s)	469, 726, 895, 1044
Bst2UI	CCWGG	1 cut(s)	1092
Bst4CI	ACNGT	3 cut(s)	58, 907, 1256
Bst6I	CTCTTC	1 cut(s)	663
BstACI	GRCGYC	1 cut(s)	333
BstAPI	GCANNNNNTGC	1 cut(s)	1167
BstBAI	YACGTR	1 cut(s)	401
BstC8I	GCNNGC	1 cut(s)	347
BstDEI	CTNAG	4 cut(s)	230, 263, 561, 1155
BstDSI	CCRYGG	3 cut(s)	513, 895, 1044
BstENI	CCTNNNNNAGG	1 cut(s)	684
BstF5I	GGATG	2 cut(s)	1002, 1217
BstFNI	CGCG	4 cut(s)	345, 347, 395, 449
BstH2I	RGCGCY	1 cut(s)	336
BstHHI	GCGC	6 cut(s)	335, 347, 349, 397, 449, 1350
BstKTI	GATC	7 cut(s)	17, 36, 51, 84, 216, 298, 547
BstMAI	GTCTC	3 cut(s)	64, 831, 1113
BstMBI	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
BstMCI	CGRYCG	3 cut(s)	8, 27, 388
BstMWI	GCNNNNNNNGC	7 cut(s)	163, 182, 355, 486, 663, 1158, 1167
BstNI	CCWGG	1 cut(s)	1092
BstNSI	RCATGY	2 cut(s)	79, 705
BstSCI	CCNGG	1 cut(s)	1090
BstSFI	CTRYAG	2 cut(s)	712, 782
BstUI	CGCG	4 cut(s)	345, 347, 395, 449
BstV1I	GCAGC	7 cut(s)	123, 464, 486, 590, 1012, 1157, 1247
BstX2I	RGATCY	2 cut(s)	213, 544
BstYI	RGATCY	2 cut(s)	213, 544
BstZI	CGGCCG	1 cut(s)	5
Bsu36I	CCTNAGG	1 cut(s)	230
BsuI	GTATCC	1 cut(s)	980
BsuRI	GGCC	5 cut(s)	7, 68, 1074, 1095, 1373
BtgI	CCRYGG	3 cut(s)	513, 895, 1044
BtgZI	GCGATG	2 cut(s)	3, 1299
BtsCI	GGATG	2 cut(s)	1002, 1217
BtsI	GCAGTG	2 cut(s)	779, 1402
BtsIMutI	CAGTG	3 cut(s)	779, 1366, 1402
BveI	ACCTGC	2 cut(s)	1175, 1244
Cac8I	GCNNGC	1 cut(s)	347
CaiI	CAGNNNCTG	2 cut(s)	571, 1260
CfoI	GCGC	6 cut(s)	335, 347, 349, 397, 449, 1350
Cfr10I	RCCGGY	2 cut(s)	22, 387
Cfr13I	GGNCC	3 cut(s)	67, 1094, 1371
Csp6I	GTAC	5 cut(s)	10, 235, 313, 391, 592
CspAI	ACCGGT	1 cut(s)	387
CviAII	CATG	5 cut(s)	76, 702, 896, 960, 1045
CviQI	GTAC	5 cut(s)	10, 235, 313, 391, 592
DdeI	CTNAG	4 cut(s)	230, 263, 561, 1155
DinI	GGCGCC	1 cut(s)	334
DpnI	GATC	7 cut(s)	16, 35, 50, 83, 215, 297, 546
DpnII	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
DriI	GACNNNNNGTC	1 cut(s)	100
EaeI	YGGCCR	1 cut(s)	5
EagI	CGGCCG	1 cut(s)	5
Eam1104I	CTCTTC	1 cut(s)	663
Eam1105I	GACNNNNNGTC	1 cut(s)	100
EarI	CTCTTC	1 cut(s)	663
EciI	GGCGGA	1 cut(s)	499
EclXI	CGGCCG	1 cut(s)	5
Eco130I	CCWWGG	4 cut(s)	469, 726, 895, 1044
Eco52I	CGGCCG	1 cut(s)	5
Eco72I	CACGTG	1 cut(s)	401
Eco81I	CCTNAGG	1 cut(s)	230
EcoNI	CCTNNNNNAGG	1 cut(s)	684
EcoRII	CCWGG	1 cut(s)	1090
EcoT14I	CCWWGG	4 cut(s)	469, 726, 895, 1044
EgeI	GGCGCC	1 cut(s)	334
EheI	GGCGCC	1 cut(s)	334
ErhI	CCWWGG	4 cut(s)	469, 726, 895, 1044
Esp3I	CGTCTC	2 cut(s)	64, 1113
FaeI	CATG	5 cut(s)	79, 705, 899, 963, 1048
FaqI	GGGAC	2 cut(s)	885, 895
FatI	CATG	5 cut(s)	75, 701, 895, 959, 1044
FauI	CCCGC	3 cut(s)	351, 473, 1363
FblI	GTMKAC	1 cut(s)	576
FokI	GGATG	2 cut(s)	1009, 1224
FspBI	CTAG	2 cut(s)	533, 890
GlaI	GCGC	6 cut(s)	334, 346, 348, 396, 448, 1349
GsaI	CCCAGC	2 cut(s)	493, 976
GsuI	CTGGAG	1 cut(s)	136
HaeII	RGCGCY	1 cut(s)	336
HaeIII	GGCC	5 cut(s)	7, 68, 1074, 1095, 1373
HapII	CCGG	7 cut(s)	23, 316, 388, 540, 858, 1112, 1296
HhaI	GCGC	6 cut(s)	335, 347, 349, 397, 449, 1350
Hin1I	GRCGYC	1 cut(s)	333
Hin1II	CATG	5 cut(s)	79, 705, 899, 963, 1048
Hin6I	GCGC	6 cut(s)	333, 345, 347, 395, 447, 1348
HinP1I	GCGC	6 cut(s)	333, 345, 347, 395, 447, 1348
HinfI	GANTC	5 cut(s)	340, 410, 422, 454, 1078
HpaII	CCGG	7 cut(s)	23, 316, 388, 540, 858, 1112, 1296
HphI	GGTGA	5 cut(s)	46, 145, 463, 529, 1345
Hpy166II	GTNNAC	3 cut(s)	577, 867, 1220
Hpy188I	TCNGA	3 cut(s)	282, 562, 1308
Hpy188III	TCNNGA	4 cut(s)	46, 211, 994, 1066
Hpy8I	GTNNAC	3 cut(s)	577, 867, 1220
Hpy99I	CGWCG	3 cut(s)	109, 245, 932
HpyCH4III	ACNGT	3 cut(s)	58, 907, 1256
HpyCH4IV	ACGT	3 cut(s)	400, 590, 874
HpyCH4V	TGCA	4 cut(s)	606, 784, 1000, 1190
HpyF10VI	GCNNNNNNNGC	7 cut(s)	163, 182, 355, 486, 663, 1158, 1167
HpyF3I	CTNAG	4 cut(s)	230, 263, 561, 1155
HpySE526I	ACGT	3 cut(s)	400, 590, 874
Hsp92I	GRCGYC	1 cut(s)	333
Hsp92II	CATG	5 cut(s)	79, 705, 899, 963, 1048
HspAI	GCGC	6 cut(s)	333, 345, 347, 395, 447, 1348
KasI	GGCGCC	1 cut(s)	332
Kzo9I	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
LmnI	GCTCC	5 cut(s)	163, 568, 1048, 1229, 1298
Lsp1109I	GCAGC	7 cut(s)	123, 464, 486, 590, 1012, 1157, 1247
LweI	GCATC	2 cut(s)	685, 987
MaeI	CTAG	2 cut(s)	533, 890
MaeII	ACGT	3 cut(s)	400, 590, 874
MaeIII	GTNAC	6 cut(s)	535, 704, 901, 1133, 1241, 1268
MalI	GATC	7 cut(s)	16, 35, 50, 83, 215, 297, 546
MboI	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
MboII	GAAGA	4 cut(s)	43, 208, 680, 764
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	2 cut(s)	213, 544
MhlI	GDGCHC	1 cut(s)	168
MluCI	AATT	2 cut(s)	807, 1038
Mly113I	GGCGCC	1 cut(s)	333
MlyI	GAGTC	3 cut(s)	334, 416, 463
MmeI	TCCRAC	1 cut(s)	390
MroXI	GAANNNNTTC	2 cut(s)	287, 756
MseI	TTAA	1 cut(s)	1402
MslI	CAYNNNNRTG	1 cut(s)	995
MspA1I	CMGCKG	2 cut(s)	114, 477
MspI	CCGG	7 cut(s)	23, 316, 388, 540, 858, 1112, 1296
MspR9I	CCNGG	1 cut(s)	1092
MunI	CAATTG	1 cut(s)	807
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	1 cut(s)	1092
MvnI	CGCG	4 cut(s)	345, 347, 395, 449
MwoI	GCNNNNNNNGC	7 cut(s)	163, 182, 355, 486, 663, 1158, 1167
NarI	GGCGCC	1 cut(s)	333
NcoI	CCATGG	2 cut(s)	895, 1044
NdeII	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
NlaIII	CATG	5 cut(s)	79, 705, 899, 963, 1048
NlaIV	GGNNCC	4 cut(s)	209, 334, 1050, 1231
NmeAIII	GCCGAG	1 cut(s)	309
NmuCI	GTSAC	5 cut(s)	535, 704, 1133, 1241, 1268
NspI	RCATGY	2 cut(s)	79, 705
PaqCI	CACCTGC	2 cut(s)	1175, 1244
PauI	GCGCGC	1 cut(s)	345
PciI	ACATGT	2 cut(s)	75, 701
PctI	GAATGC	1 cut(s)	494
PdmI	GAANNNNTTC	2 cut(s)	287, 756
PfeI	GAWTC	2 cut(s)	410, 1078
PinAI	ACCGGT	1 cut(s)	387
PleI	GAGTC	3 cut(s)	334, 416, 462
PluTI	GGCGCC	1 cut(s)	336
PmaCI	CACGTG	1 cut(s)	401
PmlI	CACGTG	1 cut(s)	401
PpsI	GAGTC	3 cut(s)	334, 416, 462
Ppu21I	YACGTR	1 cut(s)	401
PscI	ACATGT	2 cut(s)	75, 701
Psp6I	CCWGG	1 cut(s)	1090
PspCI	CACGTG	1 cut(s)	401
PspFI	CCCAGC	2 cut(s)	489, 972
PspGI	CCWGG	1 cut(s)	1090
PspN4I	GGNNCC	4 cut(s)	209, 334, 1050, 1231
PspPI	GGNCC	3 cut(s)	67, 1094, 1371
PsrI	GAACNNNNNNTAC	4 cut(s)	251, 283, 296, 328
PstI	CTGCAG	1 cut(s)	786
PstNI	CAGNNNCTG	2 cut(s)	571, 1260
PsuI	RGATCY	2 cut(s)	213, 544
PteI	GCGCGC	1 cut(s)	345
PvuII	CAGCTG	2 cut(s)	114, 477
RsaI	GTAC	5 cut(s)	11, 236, 314, 392, 593
RsaNI	GTAC	5 cut(s)	10, 235, 313, 391, 592
RseI	CAYNNNNRTG	1 cut(s)	995
SaqAI	TTAA	1 cut(s)	1402
Sau3AI	GATC	7 cut(s)	14, 33, 48, 81, 213, 295, 544
Sau96I	GGNCC	3 cut(s)	67, 1094, 1371
SchI	GAGTC	3 cut(s)	334, 416, 463
ScrFI	CCNGG	1 cut(s)	1092
SduI	GDGCHC	1 cut(s)	168
SfaNI	GCATC	2 cut(s)	685, 987
SfcI	CTRYAG	2 cut(s)	712, 782
SfoI	GGCGCC	1 cut(s)	334
SmiMI	CAYNNNNRTG	1 cut(s)	995
Sse9I	AATT	2 cut(s)	807, 1038
SsiI	CCGC	8 cut(s)	4, 358, 449, 480, 510, 654, 1020, 1356
SspDI	GGCGCC	1 cut(s)	332
SspMI	CTAG	2 cut(s)	533, 890
StyD4I	CCNGG	1 cut(s)	1090
StyI	CCWWGG	4 cut(s)	469, 726, 895, 1044
TaaI	ACNGT	3 cut(s)	58, 907, 1256
TaiI	ACGT	3 cut(s)	403, 593, 877
TaqI	TCGA	8 cut(s)	72, 80, 140, 240, 302, 633, 737, 1224
TaqII	GACCGA	1 cut(s)	266
TasI	AATT	2 cut(s)	807, 1038
TauI	GCSGC	2 cut(s)	7, 657
TfiI	GAWTC	2 cut(s)	410, 1078
Tru1I	TTAA	1 cut(s)	1402
Tru9I	TTAA	1 cut(s)	1402
TscAI	CASTG	3 cut(s)	786, 1373, 1402
TseFI	GTSAC	5 cut(s)	535, 704, 1133, 1241, 1268
TseI	GCWGC	8 cut(s)	111, 474, 477, 603, 1000, 1170, 1260, 1392
Tsp45I	GTSAC	5 cut(s)	535, 704, 1133, 1241, 1268
TspDTI	ATGAA	3 cut(s)	368, 612, 628
TspGWI	ACGGA	6 cut(s)	183, 415, 938, 945, 1095, 1314
TspRI	CASTG	3 cut(s)	786, 1373, 1402
XagI	CCTNNNNNAGG	1 cut(s)	684
XceI	RCATGY	2 cut(s)	79, 705
XmiI	GTMKAC	1 cut(s)	576
XmnI	GAANNNNTTC	2 cut(s)	287, 756
XspI	CTAG	2 cut(s)	533, 890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.