Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0012766

Physical Location & Seq

Genomic Coordinates Forward (+)

69174079 .. 69177575

Gene Structure 3497 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG487400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGGTCCGTACCGATCATCATCATCGCCGATCATGGCGATCCTCTTCCTCGCCATGTTCGCCTTGTCCTCCGCCGCCGACGACATGTCGATCATCGCCTACGACAACAGCCACGCCTCCTCTGCCGGCTGGAGGACCGACGAGGAGGTGATGTCACTGTACGAGGGGTGGTTGGCCAAGCACGGCAAGTCCTACAACGGCTTGGGAGAGAAGGAGAAGCGCTTCCAGATCTTCAAGGACAACCTCAGGTTCATCGACGACCACAACGCTCTCAACCTCAGCTACAAGCTCGGTCTGACTAAGTTCGCCGATCTGTCCAACGAGGAGTACCGGTCAACGTACCTAGGGACCAAGCCCCGCGCTCTGAATAGGCTCTCCAAGACAAAGAGCGACCGGTACGCGCCACGTGTCGGCGATCAGTTGCCTGACGCTGTTGATTGGAGGAAGGAAGGCGTCGTCAACCCTGTCAAAGACCAAGGCCAGTGTGGGAGTTGCTGGGCATTCTCAACAATCTCTGCTGTGGAAGGAATCAATAAGATAGTGACTGGCGATCTTATCTCACTATCTGAGCAGGAGTTGGTTGATTGTGATAAAACTTATAATGAAGGATGCAATGGAGGGCTCATGGACTATGCCTTCGAGTTCATCATTAACAATGGTGGCATTGACAGTGAAGATGACTATCCTTACAAAGGATATGATTCCACATGTGACACCTACAGGAAAAATGCCAAGGTGGTTTCAATTGATTCTTATGAAGATGTTCCCACATACGATGAGCAAGCGTTGCAAAAGGCTGTTGCCAATCAGCCAATAGCTGTTGCTATTGAAGGAGGAGGCAGGGAATTCCAGTTATATTCATCAGCGGGTTTGCGGGTTACGGGCTCAAATGCCAGGCCTACTGGACGGTGTGGGACAGCACTAGACCATGGTGTTACTGTTGTTGGATACGGCACAGACCATGGTGTTGATTACTGGATTGTGAGGAATTCATGGGGTGCCAGCTGGGGAGAGGCAGGGTACATCAGAATGGAGCGTAATCTGGGTAACACCGCAACCGGGAAATGTGGAATTGCAATGGAGGCTTCTTACCCCGTCAAGGTAGGACAGAATCCCCCTAACCCTGGTCCATCTCCTCCATCTCCGATAAAACCTCCTCAGGTTTGTGACAACTACTTCTCCTGCCCCGAGAGCAACACCTGCTGCTGTATCTATCAGTATGCAAACTACTGCTTTGCTTGGGGATGCTGTCCTCTTGAAGGTGCCACCTGCTGTGATGACCATTACAGTTGCTGCCCATCAGACTACCCCGTCTGCAATGTCAATGCAGGAACCTGTCAGCTGAGCAAGGGCAATCCAATGAGTGTGAAGGCATTGAAGCGCACACCCGCTAAAGCTCACTGGACTTTGGGAGGTGGTGCCAAGCACAGCAGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010494 GO:0016787 GO:0019538 GO:0030054 GO:0030163 GO:0032991 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0070011 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

479

Amino Acids

52.23

Weight (kDa)

5.4

Isoelectric Point (pI)

34.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	54 - 110	1.2e-19	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	141 - 366	9.4e-81	Papain family cysteine protease
Granulin	PF00396	400 - 447	2.6e-08	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47128 AT5G43060
fragaria_vesca	FvH4_2g33910
malus_domestica	MD08G1065500.v1.1 MD08G1093900.v1.1 MD15G1078300.v1.1
prunus_persica	Prupe.1G431000_v2.0.a1
pyrus_communis	pycom08g07660 pycom15g07310
rosa_chinensis	RchiOBHm_Chr6g0308501
rosa_laevigata	RLG00000010637
rosa_multiflora	Rmu_sc0020439.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160070
rosa_rugosa	Rorug06G0367300
rosa_samantha	Rh6AG477200 Rh6BG487400 Rh6DG478500
rosa_wichuraiana	Rw6G041640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	600
AarI	CACCTGC	2 cut(s)	1208, 1277
AasI	GACNNNNNNGTC	1 cut(s)	1310
Acc36I	ACCTGC	2 cut(s)	1208, 1277
AccB1I	GGYRCC	3 cut(s)	998, 1262, 1418
AccII	CGCG	2 cut(s)	360, 401
AciI	CCGC	7 cut(s)	72, 75, 358, 866, 874, 1053, 1389
AclWI	GGATC	1 cut(s)	34
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	2 cut(s)	845, 988
AcvI	CACGTG	1 cut(s)	407
AcyI	GRCGYC	1 cut(s)	453
AfaI	GTAC	6 cut(s)	11, 161, 329, 341, 398, 1022
AfeI	AGCGCT	1 cut(s)	221
AfiI	CCNNNNNNNGG	7 cut(s)	132, 410, 692, 1059, 1099, 1124, 1259
AflIII	ACRYGT	3 cut(s)	84, 406, 707
AgeI	ACCGGT	2 cut(s)	330, 393
AgsI	TTSAA	5 cut(s)	235, 744, 830, 1259, 1378
AhdI	GACNNNNNGTC	1 cut(s)	85
AjnI	CCWGG	2 cut(s)	893, 1123
AluBI	AGCT	6 cut(s)	282, 289, 818, 1005, 1342, 1397
AluI	AGCT	6 cut(s)	282, 289, 818, 1005, 1342, 1397
AlwI	GGATC	1 cut(s)	34
AlwNI	CAGNNNCTG	1 cut(s)	1293
Ama87I	CYCGRG	1 cut(s)	1187
Aor51HI	AGCGCT	1 cut(s)	221
AoxI	GGCC	3 cut(s)	174, 478, 896
ApeKI	GCWGC	2 cut(s)	1203, 1293
ApoI	RAATTY	2 cut(s)	845, 988
ArsI	GACNNNNNNTTYG	2 cut(s)	620, 652
AsiGI	ACCGGT	2 cut(s)	330, 393
Asp700I	GAANNNNTTC	2 cut(s)	221, 762
AspA2I	CCTAGG	1 cut(s)	343
AspLEI	GCGC	4 cut(s)	222, 362, 403, 1383
AspS9I	GGNCC	4 cut(s)	5, 135, 348, 1127
AsuC2I	CCSGG	1 cut(s)	1060
AsuHPI	GGTGA	1 cut(s)	160
AvaI	CYCGRG	1 cut(s)	1187
AvaII	GGWCC	4 cut(s)	5, 135, 348, 1127
AvrII	CCTAGG	1 cut(s)	343
AxyI	CCTNAGG	2 cut(s)	245, 1158
BalI	TGGCCA	1 cut(s)	176
BanI	GGYRCC	3 cut(s)	998, 1262, 1418
BanII	GRGCYC	2 cut(s)	624, 887
BbrPI	CACGTG	1 cut(s)	407
BbvCI	CCTCAGC	1 cut(s)	278
BbvI	GCAGC	2 cut(s)	1190, 1280
BccI	CCATC	3 cut(s)	1138, 1147, 1306
BceAI	ACGGC	3 cut(s)	199, 214, 967
BciT130I	CCWGG	2 cut(s)	895, 1125
BciVI	GTATCC	1 cut(s)	941
BcnI	CCSGG	1 cut(s)	1060
BfaI	CTAG	2 cut(s)	344, 923
BfmI	CTRYAG	1 cut(s)	718
BfoI	RGCGCY	1 cut(s)	223
BfuAI	ACCTGC	2 cut(s)	1208, 1277
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	228
BisI	GCNGC	3 cut(s)	75, 1204, 1294
BlnI	CCTAGG	1 cut(s)	343
BlpI	GCTNAGC	1 cut(s)	1343
BlsI	GCNGC	3 cut(s)	76, 1205, 1295
Bme1390I	CCNGG	3 cut(s)	895, 1060, 1125
Bme18I	GGWCC	4 cut(s)	5, 135, 348, 1127
BmeRI	GACNNNNNGTC	1 cut(s)	85
BmeT110I	CYCGRG	1 cut(s)	1187
BmgT120I	GGNCC	4 cut(s)	5, 135, 348, 1127
BmiI	GGNNCC	6 cut(s)	6, 349, 1000, 1264, 1333, 1420
BmrFI	CCNGG	3 cut(s)	895, 1060, 1125
BmsI	GCATC	2 cut(s)	599, 1235
BpmI	CTGGAG	1 cut(s)	151
Bpu10I	CCTNAGC	1 cut(s)	278
Bpu1102I	GCTNAGC	1 cut(s)	1343
BpuMI	CCSGG	1 cut(s)	1060
BsaAI	YACGTR	1 cut(s)	407
BsaBI	GATNNNNATC	2 cut(s)	19, 681
BsaHI	GRCGYC	1 cut(s)	453
BsaJI	CCNNGG	6 cut(s)	343, 475, 732, 928, 961, 1123
BsaWI	WCCGGW	2 cut(s)	330, 393
BsaXI	ACNNNNNCTCC	6 cut(s)	137, 167, 317, 347, 1163, 1193
Bsc4I	CCNNNNNNNGG	7 cut(s)	132, 410, 692, 1059, 1099, 1124, 1259
Bse118I	RCCGGY	3 cut(s)	125, 330, 393
Bse1I	ACTGG	6 cut(s)	481, 550, 850, 907, 980, 1406
Bse21I	CCTNAGG	2 cut(s)	245, 1158
Bse3DI	GCAATG	3 cut(s)	619, 1083, 1324
Bse8I	GATNNNNATC	2 cut(s)	19, 681
BseBI	CCWGG	2 cut(s)	895, 1125
BseDI	CCNNGG	6 cut(s)	343, 475, 732, 928, 961, 1123
BseGI	GGATG	2 cut(s)	614, 1250
BseJI	GATNNNNATC	2 cut(s)	19, 681
BseLI	CCNNNNNNNGG	7 cut(s)	132, 410, 692, 1059, 1099, 1124, 1259
BseMI	GCAATG	3 cut(s)	619, 1083, 1324
BseMII	CTCAG	5 cut(s)	259, 292, 558, 1172, 1334
BseNI	ACTGG	6 cut(s)	481, 550, 850, 907, 980, 1406
BseRI	GAGGAG	6 cut(s)	109, 158, 338, 849, 1125, 1146
BseXI	GCAGC	2 cut(s)	1190, 1280
BseYI	CCCAGC	2 cut(s)	495, 1005
Bsh1236I	CGCG	2 cut(s)	360, 401
Bsh1285I	CGRYCG	1 cut(s)	394
BshFI	GGCC	3 cut(s)	176, 480, 898
BshNI	GGYRCC	3 cut(s)	998, 1262, 1418
BshTI	ACCGGT	2 cut(s)	330, 393
BsiEI	CGRYCG	1 cut(s)	394
BsiHKCI	CYCGRG	1 cut(s)	1187
BsiSI	CCGG	4 cut(s)	126, 331, 394, 1059
BslFI	GGGAC	2 cut(s)	361, 928
BslI	CCNNNNNNNGG	7 cut(s)	132, 410, 692, 1059, 1099, 1124, 1259
BsmFI	GGGAC	2 cut(s)	361, 928
BsmI	GAATGC	1 cut(s)	500
BsnI	GGCC	3 cut(s)	176, 480, 898
BsoBI	CYCGRG	1 cut(s)	1187
Bsp1286I	GDGCHC	2 cut(s)	624, 887
Bsp143I	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
Bsp1720I	GCTNAGC	1 cut(s)	1343
Bsp19I	CCATGG	2 cut(s)	928, 961
BspACI	CCGC	7 cut(s)	72, 75, 358, 866, 874, 1053, 1389
BspANI	GGCC	3 cut(s)	176, 480, 898
BspCNI	CTCAG	5 cut(s)	258, 291, 559, 1171, 1335
BspFNI	CGCG	2 cut(s)	360, 401
BspLI	GGNNCC	6 cut(s)	6, 349, 1000, 1264, 1333, 1420
BspMI	ACCTGC	2 cut(s)	1208, 1277
BspPI	GGATC	1 cut(s)	34
BspT107I	GGYRCC	3 cut(s)	998, 1262, 1418
BsrDI	GCAATG	3 cut(s)	619, 1083, 1324
BsrFI	RCCGGY	3 cut(s)	125, 330, 393
BsrI	ACTGG	6 cut(s)	481, 550, 850, 907, 980, 1406
BssAI	RCCGGY	3 cut(s)	125, 330, 393
BssECI	CCNNGG	6 cut(s)	343, 475, 732, 928, 961, 1123
BssMI	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
BssNI	GRCGYC	1 cut(s)	453
BssT1I	CCWWGG	5 cut(s)	343, 475, 732, 928, 961
Bst2UI	CCWGG	2 cut(s)	895, 1125
Bst4CI	ACNGT	5 cut(s)	159, 671, 909, 940, 1289
Bst6I	CTCTTC	1 cut(s)	50
BstACI	GRCGYC	1 cut(s)	453
BstAPI	GCANNNNNTGC	2 cut(s)	787, 1200
BstBAI	YACGTR	1 cut(s)	407
BstC8I	GCNNGC	3 cut(s)	127, 783, 1003
BstDEI	CTNAG	6 cut(s)	245, 278, 300, 567, 1158, 1343
BstDSI	CCRYGG	2 cut(s)	928, 961
BstENI	CCTNNNNNAGG	2 cut(s)	690, 1257
BstF5I	GGATG	2 cut(s)	614, 1250
BstFNI	CGCG	2 cut(s)	360, 401
BstH2I	RGCGCY	1 cut(s)	223
BstHHI	GCGC	4 cut(s)	222, 362, 403, 1383
BstKTI	GATC	8 cut(s)	17, 33, 42, 93, 231, 313, 418, 553
BstMBI	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
BstMCI	CGRYCG	1 cut(s)	394
BstMWI	GCNNNNNNNGC	6 cut(s)	59, 122, 787, 1082, 1191, 1200
BstNI	CCWGG	2 cut(s)	895, 1125
BstNSI	RCATGY	2 cut(s)	88, 711
BstSCI	CCNGG	3 cut(s)	893, 1058, 1123
BstSFI	CTRYAG	1 cut(s)	718
BstUI	CGCG	2 cut(s)	360, 401
BstV1I	GCAGC	2 cut(s)	1190, 1280
BstX2I	RGATCY	1 cut(s)	228
BstYI	RGATCY	1 cut(s)	228
Bsu36I	CCTNAGG	2 cut(s)	245, 1158
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	3 cut(s)	176, 480, 898
BtgI	CCRYGG	2 cut(s)	928, 961
BtgZI	GCGATG	2 cut(s)	9, 79
BtsCI	GGATG	2 cut(s)	614, 1250
BtsIMutI	CAGTG	4 cut(s)	155, 488, 676, 1399
BveI	ACCTGC	2 cut(s)	1208, 1277
Cac8I	GCNNGC	3 cut(s)	127, 783, 1003
CaiI	CAGNNNCTG	1 cut(s)	1293
CfoI	GCGC	4 cut(s)	222, 362, 403, 1383
Cfr10I	RCCGGY	3 cut(s)	125, 330, 393
Cfr13I	GGNCC	4 cut(s)	5, 135, 348, 1127
CseI	GACGC	2 cut(s)	437, 442
Csp6I	GTAC	6 cut(s)	10, 160, 328, 340, 397, 1021
CspAI	ACCGGT	2 cut(s)	330, 393
CviAII	CATG	8 cut(s)	34, 55, 85, 625, 708, 929, 962, 993
CviQI	GTAC	6 cut(s)	10, 160, 328, 340, 397, 1021
DdeI	CTNAG	6 cut(s)	245, 278, 300, 567, 1158, 1343
DpnI	GATC	8 cut(s)	16, 32, 41, 92, 230, 312, 417, 552
DpnII	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
DrdI	GACNNNNNNGTC	1 cut(s)	1310
DriI	GACNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	1310
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	1 cut(s)	50
Eam1105I	GACNNNNNGTC	1 cut(s)	85
EarI	CTCTTC	1 cut(s)	50
EciI	GGCGGA	1 cut(s)	61
Eco130I	CCWWGG	5 cut(s)	343, 475, 732, 928, 961
Eco147I	AGGCCT	1 cut(s)	898
Eco24I	GRGCYC	2 cut(s)	624, 887
Eco47I	GGWCC	4 cut(s)	5, 135, 348, 1127
Eco47III	AGCGCT	1 cut(s)	221
Eco72I	CACGTG	1 cut(s)	407
Eco81I	CCTNAGG	2 cut(s)	245, 1158
Eco88I	CYCGRG	1 cut(s)	1187
EcoNI	CCTNNNNNAGG	2 cut(s)	690, 1257
EcoRI	GAATTC	2 cut(s)	845, 988
EcoRII	CCWGG	2 cut(s)	893, 1123
EcoT14I	CCWWGG	5 cut(s)	343, 475, 732, 928, 961
EcoT38I	GRGCYC	2 cut(s)	624, 887
ErhI	CCWWGG	5 cut(s)	343, 475, 732, 928, 961
FaeI	CATG	8 cut(s)	37, 58, 88, 628, 711, 932, 965, 996
FaqI	GGGAC	2 cut(s)	361, 928
FatI	CATG	8 cut(s)	33, 54, 84, 624, 707, 928, 961, 992
FauI	CCCGC	4 cut(s)	365, 859, 867, 1396
Fnu4HI	GCNGC	3 cut(s)	75, 1204, 1294
FokI	GGATG	2 cut(s)	621, 1257
FriOI	GRGCYC	2 cut(s)	624, 887
Fsp4HI	GCNGC	3 cut(s)	75, 1204, 1294
FspBI	CTAG	2 cut(s)	344, 923
GlaI	GCGC	4 cut(s)	221, 361, 402, 1382
GluI	GCNGC	3 cut(s)	75, 1204, 1294
GsaI	CCCAGC	2 cut(s)	499, 1009
GsuI	CTGGAG	1 cut(s)	151
HaeII	RGCGCY	1 cut(s)	223
HaeIII	GGCC	3 cut(s)	176, 480, 898
HapII	CCGG	4 cut(s)	126, 331, 394, 1059
HgaI	GACGC	2 cut(s)	437, 442
HhaI	GCGC	4 cut(s)	222, 362, 403, 1383
Hin1I	GRCGYC	1 cut(s)	453
Hin1II	CATG	8 cut(s)	37, 58, 88, 628, 711, 932, 965, 996
Hin6I	GCGC	4 cut(s)	220, 360, 401, 1381
HinP1I	GCGC	4 cut(s)	220, 360, 401, 1381
HincII	GTYRAC	2 cut(s)	336, 460
HindII	GTYRAC	2 cut(s)	336, 460
HinfI	GANTC	4 cut(s)	528, 701, 749, 1111
HpaII	CCGG	4 cut(s)	126, 331, 394, 1059
HphI	GGTGA	1 cut(s)	160
Hpy166II	GTNNAC	2 cut(s)	336, 460
Hpy188I	TCNGA	6 cut(s)	297, 366, 568, 1028, 1146, 1303
Hpy188III	TCNNGA	2 cut(s)	226, 1256
Hpy8I	GTNNAC	2 cut(s)	336, 460
Hpy99I	CGWCG	4 cut(s)	83, 143, 260, 458
HpyAV	CCTTC	9 cut(s)	205, 439, 443, 518, 599, 646, 824, 1253, 1363
HpyCH4III	ACNGT	5 cut(s)	159, 671, 909, 940, 1289
HpyCH4IV	ACGT	2 cut(s)	338, 406
HpyCH4V	TGCA	6 cut(s)	612, 790, 1076, 1223, 1317, 1328
HpyF10VI	GCNNNNNNNGC	6 cut(s)	59, 122, 787, 1082, 1191, 1200
HpyF3I	CTNAG	6 cut(s)	245, 278, 300, 567, 1158, 1343
HpySE526I	ACGT	2 cut(s)	338, 406
Hsp92I	GRCGYC	1 cut(s)	453
Hsp92II	CATG	8 cut(s)	37, 58, 88, 628, 711, 932, 965, 996
HspAI	GCGC	4 cut(s)	220, 360, 401, 1381
KroI	GCCGGC	1 cut(s)	125
KroNI	GCCGGC	1 cut(s)	127
Kzo9I	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
LmnI	GCTCC	1 cut(s)	1033
Lsp1109I	GCAGC	2 cut(s)	1190, 1280
LweI	GCATC	2 cut(s)	599, 1235
MaeI	CTAG	2 cut(s)	344, 923
MaeII	ACGT	2 cut(s)	338, 406
MaeIII	GTNAC	7 cut(s)	153, 541, 710, 877, 934, 1046, 1166
MalI	GATC	8 cut(s)	16, 32, 41, 92, 230, 312, 417, 552
MboI	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
MboII	GAAGA	4 cut(s)	37, 223, 686, 770
MfeI	CAATTG	1 cut(s)	744
MflI	RGATCY	1 cut(s)	228
MhlI	GDGCHC	2 cut(s)	624, 887
MlsI	TGGCCA	1 cut(s)	176
MluCI	AATT	4 cut(s)	744, 845, 988, 1071
MluNI	TGGCCA	1 cut(s)	176
MmeI	TCCRAC	2 cut(s)	342, 925
Mox20I	TGGCCA	1 cut(s)	176
MroNI	GCCGGC	1 cut(s)	125
MroXI	GAANNNNTTC	2 cut(s)	221, 762
MscI	TGGCCA	1 cut(s)	176
MseI	TTAA	2 cut(s)	651, 1438
MslI	CAYNNNNRTG	1 cut(s)	1028
Msp20I	TGGCCA	1 cut(s)	176
MspA1I	CMGCKG	3 cut(s)	866, 1005, 1342
MspI	CCGG	4 cut(s)	126, 331, 394, 1059
MspR9I	CCNGG	3 cut(s)	895, 1060, 1125
MunI	CAATTG	1 cut(s)	744
Mva1269I	GAATGC	1 cut(s)	500
MvaI	CCWGG	2 cut(s)	895, 1125
MvnI	CGCG	2 cut(s)	360, 401
MwoI	GCNNNNNNNGC	6 cut(s)	59, 122, 787, 1082, 1191, 1200
NaeI	GCCGGC	1 cut(s)	127
NciI	CCSGG	1 cut(s)	1060
NcoI	CCATGG	2 cut(s)	928, 961
NdeII	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
NgoMIV	GCCGGC	1 cut(s)	125
NlaIII	CATG	8 cut(s)	37, 58, 88, 628, 711, 932, 965, 996
NlaIV	GGNNCC	6 cut(s)	6, 349, 1000, 1264, 1333, 1420
NmuCI	GTSAC	4 cut(s)	153, 541, 710, 1166
NspI	RCATGY	2 cut(s)	88, 711
PaqCI	CACCTGC	2 cut(s)	1208, 1277
PceI	AGGCCT	1 cut(s)	898
PciI	ACATGT	2 cut(s)	84, 707
PctI	GAATGC	1 cut(s)	500
PdiI	GCCGGC	1 cut(s)	127
PdmI	GAANNNNTTC	2 cut(s)	221, 762
PfeI	GAWTC	4 cut(s)	528, 701, 749, 1111
PinAI	ACCGGT	2 cut(s)	330, 393
PkrI	GCNGC	3 cut(s)	76, 1205, 1295
PmaCI	CACGTG	1 cut(s)	407
PmlI	CACGTG	1 cut(s)	407
Ppu21I	YACGTR	1 cut(s)	407
PscI	ACATGT	2 cut(s)	84, 707
PsiI	TTATAA	1 cut(s)	600
Psp6I	CCWGG	2 cut(s)	893, 1123
PspCI	CACGTG	1 cut(s)	407
PspFI	CCCAGC	2 cut(s)	495, 1005
PspGI	CCWGG	2 cut(s)	893, 1123
PspN4I	GGNNCC	6 cut(s)	6, 349, 1000, 1264, 1333, 1420
PspPI	GGNCC	4 cut(s)	5, 135, 348, 1127
PstNI	CAGNNNCTG	1 cut(s)	1293
PsuI	RGATCY	1 cut(s)	228
PvuII	CAGCTG	2 cut(s)	1005, 1342
RsaI	GTAC	6 cut(s)	11, 161, 329, 341, 398, 1022
RsaNI	GTAC	6 cut(s)	10, 160, 328, 340, 397, 1021
RseI	CAYNNNNRTG	1 cut(s)	1028
SaqAI	TTAA	2 cut(s)	651, 1438
SatI	GCNGC	3 cut(s)	75, 1204, 1294
Sau3AI	GATC	8 cut(s)	14, 30, 39, 90, 228, 310, 415, 550
Sau96I	GGNCC	4 cut(s)	5, 135, 348, 1127
ScrFI	CCNGG	3 cut(s)	895, 1060, 1125
SduI	GDGCHC	2 cut(s)	624, 887
SfaNI	GCATC	2 cut(s)	599, 1235
SfcI	CTRYAG	1 cut(s)	718
SinI	GGWCC	4 cut(s)	5, 135, 348, 1127
SmiMI	CAYNNNNRTG	1 cut(s)	1028
Sse9I	AATT	4 cut(s)	744, 845, 988, 1071
SseBI	AGGCCT	1 cut(s)	898
SsiI	CCGC	7 cut(s)	72, 75, 358, 866, 874, 1053, 1389
SspMI	CTAG	2 cut(s)	344, 923
StuI	AGGCCT	1 cut(s)	898
StyD4I	CCNGG	3 cut(s)	893, 1058, 1123
StyI	CCWWGG	5 cut(s)	343, 475, 732, 928, 961
TaaI	ACNGT	5 cut(s)	159, 671, 909, 940, 1289
TaiI	ACGT	2 cut(s)	341, 409
TaqI	TCGA	3 cut(s)	89, 255, 639
TaqII	GACCGA	2 cut(s)	152, 281
TasI	AATT	4 cut(s)	744, 845, 988, 1071
TauI	GCSGC	1 cut(s)	77
TfiI	GAWTC	4 cut(s)	528, 701, 749, 1111
Tru1I	TTAA	2 cut(s)	651, 1438
Tru9I	TTAA	2 cut(s)	651, 1438
TscAI	CASTG	4 cut(s)	162, 488, 676, 1406
TseFI	GTSAC	4 cut(s)	153, 541, 710, 1166
TseI	GCWGC	2 cut(s)	1203, 1293
Tsp45I	GTSAC	4 cut(s)	153, 541, 710, 1166
TspDTI	ATGAA	6 cut(s)	241, 618, 634, 771, 849, 981
TspRI	CASTG	4 cut(s)	162, 488, 676, 1406
VpaK11BI	GGWCC	4 cut(s)	5, 135, 348, 1127
XagI	CCTNNNNNAGG	2 cut(s)	690, 1257
XapI	RAATTY	2 cut(s)	845, 988
XceI	RCATGY	2 cut(s)	88, 711
XcmI	CCANNNNNNNNNTGG	1 cut(s)	482
XmaJI	CCTAGG	1 cut(s)	343
XmnI	GAANNNNTTC	2 cut(s)	221, 762
XspI	CTAG	2 cut(s)	344, 923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.