Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012766

Physical Location & Seq

Genomic Coordinates Reverse (-)

5229470 .. 5230297

Gene Structure 828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1065500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGGGGCCGTACCGATCATGGCCGGTCGTGGCAGTCCTCATCCTCATGATCTTCACTGTCTCATCGGCCCTCGACATGTCGATCATCTCCTACGACAGCAACAACCACCTCGGAGACAACAAGTCGTTGGGCAGCTGGAGGACCGACAATGTGGTGATGTCGATATACGAGGGGTGGCTGGCGGAGCACGGAAAGGCATACAATGCTTTGGGAGAGAAGGAGAGAAGTTTCCAGATCTTCAAGGACAACCTTAGGTACATCGACGAAAAAAACTCCAAGAACCTTAGCTACAAGCTCGGTCTGAACAGGTTCGTCGATCTGTTGAACGAGGAGTACCGGAACACTTACCTCGGCGCCAAGACCCGGGCACAGATGAAGCGGGTGTCCAACAGAAACATCAAGAGCGACCGATACGCGCCACATGTAGGCGATTCGTTGCTTGACTCCGTTGATTGGAGGAAGGAGGGCGCGGTGAGTCCAGTCAAAGACCAAGGCAGCTGCGGGAGTTGCTGGACATTCTCAACTATCTCCGCCGTGGAAGGTATCAATAAGCTAGTCACCGGCGATCTCATCTCCTTATATGAGCAGGAGCTGGTAAACTACGATAGAACGTACAATGAAGGCTGCAATGGAGGTCTTACGGACTATGGCTTCAAGTTCATCATCAATAATGACGGCATTGATAGCGAAGAGGACTATCCTTACAAAGGATATGATGCCACATGTGACACCTACAGGACTCCAAAATTGATATTTTCCACAGGTTTCTTGAGCTCCAGTCCGCGCTCGAAGGGTCCGTCCCGGGAAAGGTCTCTGTTCCGCCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010494 GO:0016787 GO:0019538 GO:0030054 GO:0030163 GO:0032991 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0070011 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

276

Amino Acids

31.22

Weight (kDa)

6.84

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	56 - 112	3.4e-15	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	148 - 248	1.6e-33	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47128 AT5G43060
fragaria_vesca	FvH4_2g33910
malus_domestica	MD08G1065500.v1.1 MD08G1093900.v1.1 MD15G1078300.v1.1
prunus_persica	Prupe.1G431000_v2.0.a1
pyrus_communis	pycom08g07660 pycom15g07310
rosa_chinensis	RchiOBHm_Chr6g0308501
rosa_laevigata	RLG00000010637
rosa_multiflora	Rmu_sc0020439.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160070
rosa_rugosa	Rorug06G0367300
rosa_samantha	Rh6AG477200 Rh6BG487400 Rh6DG478500
rosa_wichuraiana	Rw6G041640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	353
AccII	CGCG	3 cut(s)	416, 470, 784
AciI	CCGC	7 cut(s)	182, 379, 470, 501, 531, 782, 820
AcoI	YGGCCR	1 cut(s)	20
AcyI	GRCGYC	1 cut(s)	354
AfaI	GTAC	4 cut(s)	11, 257, 335, 614
AfiI	CCNNNNNNNGG	5 cut(s)	363, 425, 453, 707, 807
AflIII	ACRYGT	3 cut(s)	75, 421, 722
AgsI	TTSAA	3 cut(s)	241, 325, 655
AhdI	GACNNNNNGTC	1 cut(s)	121
AluBI	AGCT	7 cut(s)	135, 288, 295, 498, 553, 592, 774
AluI	AGCT	7 cut(s)	135, 288, 295, 498, 553, 592, 774
Alw21I	GWGCWC	2 cut(s)	189, 776
Alw26I	GTCTC	3 cut(s)	64, 108, 816
AlwNI	CAGNNNCTG	1 cut(s)	592
Ama87I	CYCGRG	2 cut(s)	363, 801
AoxI	GGCC	3 cut(s)	5, 20, 66
ApeKI	GCWGC	4 cut(s)	132, 495, 498, 624
ArsI	GACNNNNNNTTYG	2 cut(s)	782, 814
Asp700I	GAANNNNTTC	1 cut(s)	308
AspLEI	GCGC	4 cut(s)	356, 418, 470, 786
AspS9I	GGNCC	4 cut(s)	5, 67, 141, 794
AsuC2I	CCSGG	4 cut(s)	364, 365, 802, 803
AsuHPI	GGTGA	3 cut(s)	166, 484, 550
AvaI	CYCGRG	2 cut(s)	363, 801
AvaII	GGWCC	2 cut(s)	141, 794
AxyI	CCTNAGG	1 cut(s)	251
BaeGI	GKGCMC	1 cut(s)	370
BanI	GGYRCC	1 cut(s)	353
BanII	GRGCYC	1 cut(s)	776
Bbv12I	GWGCWC	2 cut(s)	189, 776
BbvI	GCAGC	4 cut(s)	144, 485, 507, 611
BceAI	ACGGC	2 cut(s)	518, 691
BcnI	CCSGG	4 cut(s)	364, 365, 802, 803
BcoDI	GTCTC	3 cut(s)	64, 108, 816
BfaI	CTAG	1 cut(s)	554
BfmI	CTRYAG	1 cut(s)	733
BfoI	RGCGCY	1 cut(s)	357
BglII	AGATCT	1 cut(s)	234
BisI	GCNGC	4 cut(s)	133, 496, 499, 625
BlsI	GCNGC	4 cut(s)	134, 497, 500, 626
Bme1390I	CCNGG	4 cut(s)	364, 365, 802, 803
Bme18I	GGWCC	2 cut(s)	141, 794
BmeRI	GACNNNNNGTC	1 cut(s)	121
BmeT110I	CYCGRG	2 cut(s)	363, 801
BmgT120I	GGNCC	4 cut(s)	5, 67, 141, 794
BmiI	GGNNCC	3 cut(s)	6, 355, 795
BmrFI	CCNGG	4 cut(s)	364, 365, 802, 803
BmsI	GCATC	1 cut(s)	706
BpmI	CTGGAG	2 cut(s)	157, 760
Bpu10I	CCTNAGC	1 cut(s)	284
BpuEI	CTTGAG	1 cut(s)	790
BpuMI	CCSGG	4 cut(s)	364, 365, 802, 803
BsaHI	GRCGYC	1 cut(s)	354
BsaI	GGTCTC	1 cut(s)	816
BsaJI	CCNNGG	6 cut(s)	109, 349, 363, 490, 534, 801
BsaWI	WCCGGW	1 cut(s)	336
Bsc4I	CCNNNNNNNGG	5 cut(s)	363, 425, 453, 707, 807
Bse118I	RCCGGY	3 cut(s)	22, 560, 822
Bse1I	ACTGG	2 cut(s)	479, 777
Bse21I	CCTNAGG	1 cut(s)	251
Bse3DI	GCAATG	1 cut(s)	634
BseDI	CCNNGG	6 cut(s)	109, 349, 363, 490, 534, 801
BseGI	GGATG	1 cut(s)	39
BseLI	CCNNNNNNNGG	5 cut(s)	363, 425, 453, 707, 807
BseMI	GCAATG	1 cut(s)	634
BseNI	ACTGG	2 cut(s)	479, 777
BseRI	GAGGAG	1 cut(s)	344
BseSI	GKGCMC	1 cut(s)	370
BseXI	GCAGC	4 cut(s)	144, 485, 507, 611
Bsh1236I	CGCG	3 cut(s)	416, 470, 784
Bsh1285I	CGRYCG	2 cut(s)	27, 409
BshFI	GGCC	3 cut(s)	7, 22, 68
BshNI	GGYRCC	1 cut(s)	353
BsiEI	CGRYCG	2 cut(s)	27, 409
BsiHKAI	GWGCWC	2 cut(s)	189, 776
BsiHKCI	CYCGRG	2 cut(s)	363, 801
BsiSI	CCGG	6 cut(s)	23, 337, 364, 561, 802, 823
BslFI	GGGAC	1 cut(s)	784
BslI	CCNNNNNNNGG	5 cut(s)	363, 425, 453, 707, 807
BsmAI	GTCTC	3 cut(s)	64, 108, 816
BsmFI	GGGAC	1 cut(s)	784
BsnI	GGCC	3 cut(s)	7, 22, 68
Bso31I	GGTCTC	1 cut(s)	816
BsoBI	CYCGRG	2 cut(s)	363, 801
Bsp1286I	GDGCHC	3 cut(s)	189, 370, 776
Bsp143I	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
BspACI	CCGC	7 cut(s)	182, 379, 470, 501, 531, 782, 820
BspANI	GGCC	3 cut(s)	7, 22, 68
BspFNI	CGCG	3 cut(s)	416, 470, 784
BspHI	TCATGA	1 cut(s)	45
BspLI	GGNNCC	3 cut(s)	6, 355, 795
BspT107I	GGYRCC	1 cut(s)	353
BspTNI	GGTCTC	1 cut(s)	816
BsrDI	GCAATG	1 cut(s)	634
BsrFI	RCCGGY	3 cut(s)	22, 560, 822
BsrI	ACTGG	2 cut(s)	479, 777
BssAI	RCCGGY	3 cut(s)	22, 560, 822
BssECI	CCNNGG	6 cut(s)	109, 349, 363, 490, 534, 801
BssMI	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
BssNI	GRCGYC	1 cut(s)	354
BssT1I	CCWWGG	1 cut(s)	490
Bst4CI	ACNGT	1 cut(s)	58
Bst6I	CTCTTC	1 cut(s)	684
BstACI	GRCGYC	1 cut(s)	354
BstAPI	GCANNNNNTGC	1 cut(s)	203
BstC8I	GCNNGC	1 cut(s)	180
BstDEI	CTNAG	2 cut(s)	251, 284
BstDSI	CCRYGG	1 cut(s)	534
BstENI	CCTNNNNNAGG	1 cut(s)	705
BstF5I	GGATG	1 cut(s)	39
BstFNI	CGCG	3 cut(s)	416, 470, 784
BstH2I	RGCGCY	1 cut(s)	357
BstHHI	GCGC	4 cut(s)	356, 418, 470, 786
BstKTI	GATC	6 cut(s)	17, 51, 84, 237, 319, 568
BstMAI	GTCTC	3 cut(s)	64, 108, 816
BstMBI	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
BstMCI	CGRYCG	2 cut(s)	27, 409
BstMWI	GCNNNNNNNGC	4 cut(s)	184, 203, 507, 684
BstNSI	RCATGY	3 cut(s)	79, 425, 726
BstSCI	CCNGG	4 cut(s)	362, 363, 800, 801
BstSFI	CTRYAG	1 cut(s)	733
BstSLI	GKGCMC	1 cut(s)	370
BstUI	CGCG	3 cut(s)	416, 470, 784
BstV1I	GCAGC	4 cut(s)	144, 485, 507, 611
BstX2I	RGATCY	1 cut(s)	234
BstYI	RGATCY	1 cut(s)	234
Bsu36I	CCTNAGG	1 cut(s)	251
BsuRI	GGCC	3 cut(s)	7, 22, 68
BtgI	CCRYGG	1 cut(s)	534
BtsCI	GGATG	1 cut(s)	39
BtsIMutI	CAGTG	1 cut(s)	54
Cac8I	GCNNGC	1 cut(s)	180
CaiI	CAGNNNCTG	1 cut(s)	592
CciI	TCATGA	1 cut(s)	45
CfoI	GCGC	4 cut(s)	356, 418, 470, 786
Cfr10I	RCCGGY	3 cut(s)	22, 560, 822
Cfr13I	GGNCC	4 cut(s)	5, 67, 141, 794
Cfr9I	CCCGGG	2 cut(s)	363, 801
Csp6I	GTAC	4 cut(s)	10, 256, 334, 613
CviAII	CATG	5 cut(s)	18, 46, 76, 422, 723
CviQI	GTAC	4 cut(s)	10, 256, 334, 613
DdeI	CTNAG	2 cut(s)	251, 284
DinI	GGCGCC	1 cut(s)	355
DpnI	GATC	6 cut(s)	16, 50, 83, 236, 318, 567
DpnII	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
DriI	GACNNNNNGTC	1 cut(s)	121
EaeI	YGGCCR	1 cut(s)	20
Eam1104I	CTCTTC	1 cut(s)	684
Eam1105I	GACNNNNNGTC	1 cut(s)	121
EarI	CTCTTC	1 cut(s)	684
EciI	GGCGGA	3 cut(s)	197, 520, 809
Ecl136II	GAGCTC	1 cut(s)	774
Eco130I	CCWWGG	1 cut(s)	490
Eco24I	GRGCYC	1 cut(s)	776
Eco31I	GGTCTC	1 cut(s)	816
Eco47I	GGWCC	2 cut(s)	141, 794
Eco53kI	GAGCTC	1 cut(s)	774
Eco81I	CCTNAGG	1 cut(s)	251
Eco88I	CYCGRG	2 cut(s)	363, 801
EcoICRI	GAGCTC	1 cut(s)	774
EcoNI	CCTNNNNNAGG	1 cut(s)	705
EcoT14I	CCWWGG	1 cut(s)	490
EcoT38I	GRGCYC	1 cut(s)	776
EgeI	GGCGCC	1 cut(s)	355
EheI	GGCGCC	1 cut(s)	355
ErhI	CCWWGG	1 cut(s)	490
FaeI	CATG	5 cut(s)	21, 49, 79, 425, 726
FaqI	GGGAC	1 cut(s)	784
FatI	CATG	5 cut(s)	17, 45, 75, 421, 722
FauI	CCCGC	2 cut(s)	372, 494
Fnu4HI	GCNGC	4 cut(s)	133, 496, 499, 625
FokI	GGATG	1 cut(s)	26
FriOI	GRGCYC	1 cut(s)	776
Fsp4HI	GCNGC	4 cut(s)	133, 496, 499, 625
FspBI	CTAG	1 cut(s)	554
GlaI	GCGC	4 cut(s)	355, 417, 469, 785
GluI	GCNGC	4 cut(s)	133, 496, 499, 625
GsuI	CTGGAG	2 cut(s)	157, 760
HaeII	RGCGCY	1 cut(s)	357
HaeIII	GGCC	3 cut(s)	7, 22, 68
HapII	CCGG	6 cut(s)	23, 337, 364, 561, 802, 823
HhaI	GCGC	4 cut(s)	356, 418, 470, 786
Hin1I	GRCGYC	1 cut(s)	354
Hin1II	CATG	5 cut(s)	21, 49, 79, 425, 726
Hin6I	GCGC	4 cut(s)	354, 416, 468, 784
HinP1I	GCGC	4 cut(s)	354, 416, 468, 784
HinfI	GANTC	4 cut(s)	431, 443, 475, 739
HpaII	CCGG	6 cut(s)	23, 337, 364, 561, 802, 823
HphI	GGTGA	3 cut(s)	166, 484, 550
Hpy166II	GTNNAC	1 cut(s)	598
Hpy188I	TCNGA	2 cut(s)	113, 303
Hpy188III	TCNNGA	4 cut(s)	46, 232, 400, 769
Hpy8I	GTNNAC	1 cut(s)	598
Hpy99I	CGWCG	2 cut(s)	266, 317
HpyAV	CCTTC	5 cut(s)	211, 454, 533, 614, 784
HpyCH4III	ACNGT	1 cut(s)	58
HpyCH4IV	ACGT	1 cut(s)	611
HpyCH4V	TGCA	1 cut(s)	627
HpyF10VI	GCNNNNNNNGC	4 cut(s)	184, 203, 507, 684
HpyF3I	CTNAG	2 cut(s)	251, 284
HpySE526I	ACGT	1 cut(s)	611
Hsp92I	GRCGYC	1 cut(s)	354
Hsp92II	CATG	5 cut(s)	21, 49, 79, 425, 726
HspAI	GCGC	4 cut(s)	354, 416, 468, 784
KasI	GGCGCC	1 cut(s)	353
Kzo9I	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
LmnI	GCTCC	3 cut(s)	184, 589, 779
Lsp1109I	GCAGC	4 cut(s)	144, 485, 507, 611
LweI	GCATC	1 cut(s)	706
MaeI	CTAG	1 cut(s)	554
MaeII	ACGT	1 cut(s)	611
MaeIII	GTNAC	2 cut(s)	556, 725
MalI	GATC	6 cut(s)	16, 50, 83, 236, 318, 567
MboI	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
MboII	GAAGA	3 cut(s)	43, 229, 701
MflI	RGATCY	1 cut(s)	234
MhlI	GDGCHC	3 cut(s)	189, 370, 776
MluCI	AATT	1 cut(s)	746
Mly113I	GGCGCC	1 cut(s)	354
MlyI	GAGTC	3 cut(s)	437, 484, 733
MmeI	TCCRAC	1 cut(s)	411
MroXI	GAANNNNTTC	1 cut(s)	308
MslI	CAYNNNNRTG	1 cut(s)	44
MspA1I	CMGCKG	2 cut(s)	135, 498
MspI	CCGG	6 cut(s)	23, 337, 364, 561, 802, 823
MspR9I	CCNGG	4 cut(s)	364, 365, 802, 803
MvnI	CGCG	3 cut(s)	416, 470, 784
MwoI	GCNNNNNNNGC	4 cut(s)	184, 203, 507, 684
NarI	GGCGCC	1 cut(s)	354
NciI	CCSGG	4 cut(s)	364, 365, 802, 803
NdeII	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
NlaIII	CATG	5 cut(s)	21, 49, 79, 425, 726
NlaIV	GGNNCC	3 cut(s)	6, 355, 795
NmeAIII	GCCGAG	1 cut(s)	330
NmuCI	GTSAC	2 cut(s)	556, 725
NspI	RCATGY	3 cut(s)	79, 425, 726
PagI	TCATGA	1 cut(s)	45
PciI	ACATGT	3 cut(s)	75, 421, 722
PcsI	WCGNNNNNNNCGW	1 cut(s)	794
PdmI	GAANNNNTTC	1 cut(s)	308
PfeI	GAWTC	1 cut(s)	431
PkrI	GCNGC	4 cut(s)	134, 497, 500, 626
PleI	GAGTC	3 cut(s)	437, 483, 733
PluTI	GGCGCC	1 cut(s)	357
PpsI	GAGTC	3 cut(s)	437, 483, 733
PscI	ACATGT	3 cut(s)	75, 421, 722
Psp124BI	GAGCTC	1 cut(s)	776
PspN4I	GGNNCC	3 cut(s)	6, 355, 795
PspPI	GGNCC	4 cut(s)	5, 67, 141, 794
PsrI	GAACNNNNNNTAC	4 cut(s)	272, 304, 317, 349
PstNI	CAGNNNCTG	1 cut(s)	592
PsuI	RGATCY	1 cut(s)	234
PvuII	CAGCTG	2 cut(s)	135, 498
RsaI	GTAC	4 cut(s)	11, 257, 335, 614
RsaNI	GTAC	4 cut(s)	10, 256, 334, 613
RseI	CAYNNNNRTG	1 cut(s)	44
SacI	GAGCTC	1 cut(s)	776
SatI	GCNGC	4 cut(s)	133, 496, 499, 625
Sau3AI	GATC	6 cut(s)	14, 48, 81, 234, 316, 565
Sau96I	GGNCC	4 cut(s)	5, 67, 141, 794
SchI	GAGTC	3 cut(s)	437, 484, 733
ScrFI	CCNGG	4 cut(s)	364, 365, 802, 803
SduI	GDGCHC	3 cut(s)	189, 370, 776
SfaNI	GCATC	1 cut(s)	706
SfcI	CTRYAG	1 cut(s)	733
SfoI	GGCGCC	1 cut(s)	355
SgrAI	CRCCGGYG	1 cut(s)	560
SinI	GGWCC	2 cut(s)	141, 794
SmaI	CCCGGG	2 cut(s)	365, 803
SmiMI	CAYNNNNRTG	1 cut(s)	44
SmlI	CTYRAG	1 cut(s)	769
SmoI	CTYRAG	1 cut(s)	769
Sse9I	AATT	1 cut(s)	746
SsiI	CCGC	7 cut(s)	182, 379, 470, 501, 531, 782, 820
SspDI	GGCGCC	1 cut(s)	353
SspMI	CTAG	1 cut(s)	554
SstI	GAGCTC	1 cut(s)	776
StyD4I	CCNGG	4 cut(s)	362, 363, 800, 801
StyI	CCWWGG	1 cut(s)	490
TaaI	ACNGT	1 cut(s)	58
TaiI	ACGT	1 cut(s)	614
TaqI	TCGA	6 cut(s)	72, 80, 161, 261, 315, 788
TaqII	GACCGA	3 cut(s)	158, 287, 423
TasI	AATT	1 cut(s)	746
TfiI	GAWTC	1 cut(s)	431
TscAI	CASTG	1 cut(s)	61
TseFI	GTSAC	2 cut(s)	556, 725
TseI	GCWGC	4 cut(s)	132, 495, 498, 624
Tsp45I	GTSAC	2 cut(s)	556, 725
TspDTI	ATGAA	3 cut(s)	389, 633, 649
TspGWI	ACGGA	4 cut(s)	204, 436, 656, 786
TspMI	CCCGGG	2 cut(s)	363, 801
TspRI	CASTG	1 cut(s)	61
VpaK11BI	GGWCC	2 cut(s)	141, 794
XagI	CCTNNNNNAGG	1 cut(s)	705
XceI	RCATGY	3 cut(s)	79, 425, 726
XmaI	CCCGGG	2 cut(s)	363, 801
XmnI	GAANNNNTTC	1 cut(s)	308
XspI	CTAG	1 cut(s)	554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.