Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012766

Physical Location & Seq

Genomic Coordinates Reverse (-)

7808391 .. 7812462

Gene Structure 4072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1093900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGGGCCGTACCGATCGTCGCCGGCCGTGGCGGTCCTCCTCCTCACGATCTTCACTGTCTCATCGGCCCTCGACATGTCCATCATCTCCTACGACAACAGCCTCGGAGACGGCAAGTCCACGGGCAGCTGGAGGACCGACGATGAGGTGATGTCGATTTACGAGGGGTGGCTGGCGGAGCACGGCAAGGCATACAACGCCTTGGGAGAGAAGGAGCGGCGGTTCCAGATCTTTAAAGACAACCTCAGGTACATCGACGAACAGAACTCGAAGAACCTCAGCTACAAGCTCGGTCTGAACCGGTTCGCTGATCTGTCGAACGATGAGTACCGGAACACTTACCTTGGCACCAAGACCCGCGCTCAGAAGAAACGGCTATCCAACAGGAACACCAAGAGCGACCGGTACGCGCCGCGTGTCGGCGATTCGTTGCCTGACTCCATTGACTGGAGGGAGGAGGGCGCAGTTAACCCAATCAAAGACCAAGGCAGCTGCGGGAGTTGCTGGGCATTCTCAACTATCTCCGCCGTGGAAGGTGTCAATAAGCTAGTGACCGGGGACCTTATCTCACTATCTGAGCAGGAGCTGGTAGACTGCGATACAACATACAATGAAGGATGCAATGGAGGTCTCATGGACTACGCCTTCGAGTTCATCATCAAAAATGGCGGAATTGACAGCGAAGAGGACTATCCTTACAAAGGATACGATGCCACATGTGACCCCTACAGGAAAAATGCCAGGGTGGTTTCGATCGACACTTATGAAGATGTTCCTGTAAACAGTGAGAATGCATTGAAAAAGGCTGTTGCCAATCAGCCAATCGCTGTTGCCATTGAAGGAGGTGGCAGAGACTTCCAGTTGTATAACTCCGGTGTATTTACAGGACGTTGTGGGACGGCATTGGACCATGGTGTGACAGTTGTGGGATACGGCACAGACAAGGGGCTTGATTACTGGATAGTGAGAAACTCATGGGGTGCAAGCTGGGGAGAGAAGGGATACATCAGGATGCAGCGTAATCTGGGCAACACCGCTAATGGGATATGTGGAATTGCCATGGAGCCTTCTTACCCGATAAAGAAAGGCCAGAATCCCCCTAACCCCGGTCCTTCTCCTCCATCTCCGATAAAGCCCCCGTCAGTTTGTGACAACTACTACTCCTGCCCTGAGAGCAACACGTGCTGCTGCATCTACCAGTTTGGAAACTATTGCTTTGATTGGGGATGTTGTCCACTTGAAGGTGCCACCTGCTGTGATGACCATTACAGTTGCTGCCCAAGCGAATATCCTGTCTGCGATGTCAATGCCGGAGCTTGTTTGATGAGCAAGGGCAATCCCTTGGGAGTGAAGGCATTGAAGCGCACCCCCGCGAAACCCCACTGGGCTGTTGGTGGCGGAAAGAGCAGCAGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010494 GO:0016787 GO:0019538 GO:0030054 GO:0030163 GO:0032991 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0070011 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

473

Amino Acids

51.63

Weight (kDa)

5.22

Isoelectric Point (pI)

34.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	54 - 110	6.9e-18	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	144 - 360	1.1e-84	Papain family cysteine protease
Granulin	PF00396	394 - 441	1.6e-08	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47128 AT5G43060
fragaria_vesca	FvH4_2g33910
malus_domestica	MD08G1065500.v1.1 MD08G1093900.v1.1 MD15G1078300.v1.1
prunus_persica	Prupe.1G431000_v2.0.a1
pyrus_communis	pycom08g07660 pycom15g07310
rosa_chinensis	RchiOBHm_Chr6g0308501
rosa_laevigata	RLG00000010637
rosa_multiflora	Rmu_sc0020439.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160070
rosa_rugosa	Rorug06G0367300
rosa_samantha	Rh6AG477200 Rh6BG487400 Rh6DG478500
rosa_wichuraiana	Rw6G041640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1259
Acc36I	ACCTGC	1 cut(s)	1259
AccB1I	GGYRCC	2 cut(s)	347, 1244
AccB7I	CCANNNNNTGG	1 cut(s)	447
AccBSI	CCGCTC	1 cut(s)	217
AccI	GTMKAC	1 cut(s)	591
AccII	CGCG	4 cut(s)	360, 410, 415, 1373
AcoI	YGGCCR	1 cut(s)	24
AcvI	CACGTG	1 cut(s)	1182
AfaI	GTAC	4 cut(s)	11, 251, 329, 407
AfiI	CCNNNNNNNGG	6 cut(s)	419, 447, 701, 1041, 1106, 1241
AflIII	ACRYGT	3 cut(s)	75, 716, 1179
AgeI	ACCGGT	2 cut(s)	300, 402
AgsI	TTSAA	4 cut(s)	799, 839, 1241, 1360
AhdI	GACNNNNNGTC	1 cut(s)	115
AjnI	CCWGG	1 cut(s)	740
AloI	GAACNNNNNNTCC	2 cut(s)	206, 238
AluBI	AGCT	8 cut(s)	129, 282, 289, 492, 547, 586, 987, 1316
AluI	AGCT	8 cut(s)	129, 282, 289, 492, 547, 586, 987, 1316
Alw21I	GWGCWC	1 cut(s)	183
Alw26I	GTCTC	4 cut(s)	64, 102, 635, 846
AlwNI	CAGNNNCTG	2 cut(s)	586, 1275
AoxI	GGCC	4 cut(s)	5, 24, 66, 1087
ApeKI	GCWGC	8 cut(s)	126, 489, 492, 1015, 1185, 1188, 1275, 1407
ArsI	GACNNNNNNTTYG	4 cut(s)	629, 661, 1278, 1310
AsiGI	ACCGGT	2 cut(s)	300, 402
Asp700I	GAANNNNTTC	2 cut(s)	302, 771
AspLEI	GCGC	4 cut(s)	362, 412, 464, 1365
AspS9I	GGNCC	7 cut(s)	5, 34, 67, 135, 559, 907, 1109
AsuC2I	CCSGG	2 cut(s)	556, 1107
AsuHPI	GGTGA	1 cut(s)	160
AvaII	GGWCC	5 cut(s)	34, 135, 559, 907, 1109
AxyI	CCTNAGG	1 cut(s)	245
BanI	GGYRCC	2 cut(s)	347, 1244
BbrPI	CACGTG	1 cut(s)	1182
Bbv12I	GWGCWC	1 cut(s)	183
BbvCI	CCTCAGC	1 cut(s)	278
BbvI	GCAGC	7 cut(s)	138, 479, 501, 1027, 1172, 1175, 1262
BccI	CCATC	2 cut(s)	89, 1129
BceAI	ACGGC	7 cut(s)	11, 127, 199, 389, 512, 915, 949
BcgI	CGANNNNNNTGC	2 cut(s)	1289, 1323
BciT130I	CCWGG	1 cut(s)	742
BciVI	GTATCC	3 cut(s)	698, 923, 995
BcnI	CCSGG	2 cut(s)	556, 1107
BcoDI	GTCTC	4 cut(s)	64, 102, 635, 846
BfaI	CTAG	1 cut(s)	548
BfmI	CTRYAG	1 cut(s)	727
BfuAI	ACCTGC	1 cut(s)	1259
BfuI	GTATCC	3 cut(s)	698, 923, 995
BglII	AGATCT	1 cut(s)	228
Bme1390I	CCNGG	3 cut(s)	556, 742, 1107
Bme18I	GGWCC	5 cut(s)	34, 135, 559, 907, 1109
BmeRI	GACNNNNNGTC	1 cut(s)	115
BmgT120I	GGNCC	7 cut(s)	5, 34, 67, 135, 559, 907, 1109
BmiI	GGNNCC	6 cut(s)	6, 224, 349, 560, 1065, 1246
BmrFI	CCNGG	3 cut(s)	556, 742, 1107
BmrI	ACTGGG	1 cut(s)	1393
BmsI	GCATC	4 cut(s)	608, 700, 1002, 1200
BmuI	ACTGGG	1 cut(s)	1393
BpmI	CTGGAG	2 cut(s)	151, 469
Bpu10I	CCTNAGC	1 cut(s)	278
BpuMI	CCSGG	2 cut(s)	556, 1107
BsaAI	YACGTR	1 cut(s)	1182
BsaI	GGTCTC	1 cut(s)	635
BsaWI	WCCGGW	4 cut(s)	300, 330, 402, 872
BsaXI	ACNNNNNCTCC	4 cut(s)	206, 236, 1145, 1175
Bsc4I	CCNNNNNNNGG	6 cut(s)	419, 447, 701, 1041, 1106, 1241
Bse118I	RCCGGY	3 cut(s)	22, 300, 402
Bse1I	ACTGG	5 cut(s)	452, 859, 962, 1198, 1388
Bse21I	CCTNAGG	1 cut(s)	245
Bse3DI	GCAATG	1 cut(s)	628
BseBI	CCWGG	1 cut(s)	742
BseGI	GGATG	3 cut(s)	623, 1017, 1232
BseLI	CCNNNNNNNGG	6 cut(s)	419, 447, 701, 1041, 1106, 1241
BseMI	GCAATG	1 cut(s)	628
BseMII	CTCAG	5 cut(s)	259, 292, 377, 567, 1161
BseNI	ACTGG	5 cut(s)	452, 859, 962, 1198, 1388
BseRI	GAGGAG	4 cut(s)	29, 32, 470, 1107
BseX3I	CGGCCG	1 cut(s)	24
BseXI	GCAGC	7 cut(s)	138, 479, 501, 1027, 1172, 1175, 1262
BseYI	CCCAGC	2 cut(s)	504, 987
Bsh1236I	CGCG	4 cut(s)	360, 410, 415, 1373
Bsh1285I	CGRYCG	4 cut(s)	17, 27, 403, 756
BshFI	GGCC	4 cut(s)	7, 26, 68, 1089
BshNI	GGYRCC	2 cut(s)	347, 1244
BshTI	ACCGGT	2 cut(s)	300, 402
BsiEI	CGRYCG	4 cut(s)	17, 27, 403, 756
BsiHKAI	GWGCWC	1 cut(s)	183
BsiSI	CCGG	8 cut(s)	23, 301, 331, 403, 555, 873, 1107, 1311
BslFI	GGGAC	2 cut(s)	572, 910
BslI	CCNNNNNNNGG	6 cut(s)	419, 447, 701, 1041, 1106, 1241
BsmAI	GTCTC	4 cut(s)	64, 102, 635, 846
BsmBI	CGTCTC	1 cut(s)	102
BsmFI	GGGAC	2 cut(s)	572, 910
BsmI	GAATGC	2 cut(s)	509, 796
BsnI	GGCC	4 cut(s)	7, 26, 68, 1089
Bso31I	GGTCTC	1 cut(s)	635
Bsp1286I	GDGCHC	1 cut(s)	183
Bsp143I	GATC	5 cut(s)	14, 48, 228, 310, 753
Bsp19I	CCATGG	2 cut(s)	910, 1059
BspANI	GGCC	4 cut(s)	7, 26, 68, 1089
BspCNI	CTCAG	5 cut(s)	258, 291, 376, 568, 1162
BspFNI	CGCG	4 cut(s)	360, 410, 415, 1373
BspLI	GGNNCC	6 cut(s)	6, 224, 349, 560, 1065, 1246
BspMI	ACCTGC	1 cut(s)	1259
BspT107I	GGYRCC	2 cut(s)	347, 1244
BspTNI	GGTCTC	1 cut(s)	635
BsrBI	CCGCTC	1 cut(s)	217
BsrDI	GCAATG	1 cut(s)	628
BsrFI	RCCGGY	3 cut(s)	22, 300, 402
BsrI	ACTGG	5 cut(s)	452, 859, 962, 1198, 1388
BssAI	RCCGGY	3 cut(s)	22, 300, 402
BssMI	GATC	5 cut(s)	14, 48, 228, 310, 753
BssT1I	CCWWGG	6 cut(s)	201, 343, 484, 910, 1059, 1341
Bst2UI	CCWGG	1 cut(s)	742
Bst4CI	ACNGT	4 cut(s)	58, 785, 922, 1271
Bst6I	CTCTTC	1 cut(s)	678
BstAPI	GCANNNNNTGC	1 cut(s)	1182
BstBAI	YACGTR	1 cut(s)	1182
BstC8I	GCNNGC	3 cut(s)	24, 174, 985
BstDEI	CTNAG	5 cut(s)	245, 278, 363, 576, 1170
BstDSI	CCRYGG	5 cut(s)	27, 120, 528, 910, 1059
BstENI	CCTNNNNNAGG	1 cut(s)	699
BstF5I	GGATG	3 cut(s)	623, 1017, 1232
BstFNI	CGCG	4 cut(s)	360, 410, 415, 1373
BstHHI	GCGC	4 cut(s)	362, 412, 464, 1365
BstKTI	GATC	5 cut(s)	17, 51, 231, 313, 756
BstMAI	GTCTC	4 cut(s)	64, 102, 635, 846
BstMBI	GATC	5 cut(s)	14, 48, 228, 310, 753
BstMCI	CGRYCG	4 cut(s)	17, 27, 403, 756
BstMWI	GCNNNNNNNGC	7 cut(s)	178, 197, 501, 1173, 1182, 1281, 1404
BstNI	CCWGG	1 cut(s)	742
BstNSI	RCATGY	2 cut(s)	79, 720
BstSCI	CCNGG	3 cut(s)	554, 740, 1105
BstSFI	CTRYAG	1 cut(s)	727
BstUI	CGCG	4 cut(s)	360, 410, 415, 1373
BstV1I	GCAGC	7 cut(s)	138, 479, 501, 1027, 1172, 1175, 1262
BstX2I	RGATCY	1 cut(s)	228
BstYI	RGATCY	1 cut(s)	228
BstZI	CGGCCG	1 cut(s)	24
Bsu36I	CCTNAGG	1 cut(s)	245
BsuI	GTATCC	3 cut(s)	698, 923, 995
BsuRI	GGCC	4 cut(s)	7, 26, 68, 1089
BtgI	CCRYGG	5 cut(s)	27, 120, 528, 910, 1059
BtgZI	GCGATG	1 cut(s)	1314
BtsCI	GGATG	3 cut(s)	623, 1017, 1232
BtsI	GCAGTG	1 cut(s)	1417
BtsIMutI	CAGTG	4 cut(s)	54, 790, 1381, 1417
BveI	ACCTGC	1 cut(s)	1259
Cac8I	GCNNGC	3 cut(s)	24, 174, 985
CaiI	CAGNNNCTG	2 cut(s)	586, 1275
CfoI	GCGC	4 cut(s)	362, 412, 464, 1365
Cfr10I	RCCGGY	3 cut(s)	22, 300, 402
Cfr13I	GGNCC	7 cut(s)	5, 34, 67, 135, 559, 907, 1109
Csp6I	GTAC	4 cut(s)	10, 250, 328, 406
CspAI	ACCGGT	2 cut(s)	300, 402
CviAII	CATG	6 cut(s)	76, 634, 717, 911, 975, 1060
CviQI	GTAC	4 cut(s)	10, 250, 328, 406
DdeI	CTNAG	5 cut(s)	245, 278, 363, 576, 1170
DpnI	GATC	5 cut(s)	16, 50, 230, 312, 755
DpnII	GATC	5 cut(s)	14, 48, 228, 310, 753
DraI	TTTAAA	1 cut(s)	235
DriI	GACNNNNNGTC	1 cut(s)	115
EaeI	YGGCCR	1 cut(s)	24
EagI	CGGCCG	1 cut(s)	24
Eam1104I	CTCTTC	1 cut(s)	678
Eam1105I	GACNNNNNGTC	1 cut(s)	115
EarI	CTCTTC	1 cut(s)	678
EciI	GGCGGA	4 cut(s)	191, 514, 684, 1413
EclXI	CGGCCG	1 cut(s)	24
Eco130I	CCWWGG	6 cut(s)	201, 343, 484, 910, 1059, 1341
Eco31I	GGTCTC	1 cut(s)	635
Eco47I	GGWCC	5 cut(s)	34, 135, 559, 907, 1109
Eco52I	CGGCCG	1 cut(s)	24
Eco72I	CACGTG	1 cut(s)	1182
Eco81I	CCTNAGG	1 cut(s)	245
EcoNI	CCTNNNNNAGG	1 cut(s)	699
EcoO109I	RGGNCCY	1 cut(s)	559
EcoRII	CCWGG	1 cut(s)	740
EcoT14I	CCWWGG	6 cut(s)	201, 343, 484, 910, 1059, 1341
EcoT22I	ATGCAT	1 cut(s)	796
ErhI	CCWWGG	6 cut(s)	201, 343, 484, 910, 1059, 1341
Esp3I	CGTCTC	1 cut(s)	102
FaeI	CATG	6 cut(s)	79, 637, 720, 914, 978, 1063
FaqI	GGGAC	2 cut(s)	572, 910
FatI	CATG	6 cut(s)	75, 633, 716, 910, 974, 1059
FauI	CCCGC	3 cut(s)	365, 488, 1378
FblI	GTMKAC	1 cut(s)	591
FokI	GGATG	3 cut(s)	630, 1024, 1239
FspBI	CTAG	1 cut(s)	548
GlaI	GCGC	4 cut(s)	361, 411, 463, 1364
GsaI	CCCAGC	2 cut(s)	508, 991
GsuI	CTGGAG	2 cut(s)	151, 469
HaeIII	GGCC	4 cut(s)	7, 26, 68, 1089
HapII	CCGG	8 cut(s)	23, 301, 331, 403, 555, 873, 1107, 1311
HhaI	GCGC	4 cut(s)	362, 412, 464, 1365
Hin1II	CATG	6 cut(s)	79, 637, 720, 914, 978, 1063
Hin6I	GCGC	4 cut(s)	360, 410, 462, 1363
HinP1I	GCGC	4 cut(s)	360, 410, 462, 1363
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HinfI	GANTC	3 cut(s)	425, 437, 1093
HpaI	GTTAAC	1 cut(s)	469
HpaII	CCGG	8 cut(s)	23, 301, 331, 403, 555, 873, 1107, 1311
HphI	GGTGA	1 cut(s)	160
Hpy166II	GTNNAC	5 cut(s)	120, 469, 592, 781, 1235
Hpy188I	TCNGA	5 cut(s)	107, 297, 366, 577, 1128
Hpy188III	TCNNGA	3 cut(s)	46, 226, 1009
Hpy8I	GTNNAC	5 cut(s)	120, 469, 592, 781, 1235
Hpy99I	CGWCG	3 cut(s)	22, 143, 260
HpyCH4III	ACNGT	4 cut(s)	58, 785, 922, 1271
HpyCH4IV	ACGT	2 cut(s)	889, 1181
HpyCH4V	TGCA	5 cut(s)	621, 794, 983, 1015, 1191
HpyF10VI	GCNNNNNNNGC	7 cut(s)	178, 197, 501, 1173, 1182, 1281, 1404
HpyF3I	CTNAG	5 cut(s)	245, 278, 363, 576, 1170
HpySE526I	ACGT	2 cut(s)	889, 1181
Hsp92II	CATG	6 cut(s)	79, 637, 720, 914, 978, 1063
HspAI	GCGC	4 cut(s)	360, 410, 462, 1363
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
KspAI	GTTAAC	1 cut(s)	469
Kzo9I	GATC	5 cut(s)	14, 48, 228, 310, 753
LmnI	GCTCC	5 cut(s)	178, 214, 583, 1063, 1313
Lsp1109I	GCAGC	7 cut(s)	138, 479, 501, 1027, 1172, 1175, 1262
LweI	GCATC	4 cut(s)	608, 700, 1002, 1200
MaeI	CTAG	1 cut(s)	548
MaeII	ACGT	2 cut(s)	889, 1181
MaeIII	GTNAC	4 cut(s)	550, 719, 916, 1148
MalI	GATC	5 cut(s)	16, 50, 230, 312, 755
MbiI	CCGCTC	1 cut(s)	217
MboI	GATC	5 cut(s)	14, 48, 228, 310, 753
MboII	GAAGA	5 cut(s)	43, 283, 379, 695, 779
MflI	RGATCY	1 cut(s)	228
MhlI	GDGCHC	1 cut(s)	183
MluCI	AATT	2 cut(s)	672, 1053
MlyI	GAGTC	1 cut(s)	431
MmeI	TCCRAC	1 cut(s)	405
Mph1103I	ATGCAT	1 cut(s)	796
MroNI	GCCGGC	1 cut(s)	22
MroXI	GAANNNNTTC	2 cut(s)	302, 771
MseI	TTAA	3 cut(s)	234, 468, 1417
MslI	CAYNNNNRTG	1 cut(s)	1010
MspA1I	CMGCKG	2 cut(s)	129, 492
MspI	CCGG	8 cut(s)	23, 301, 331, 403, 555, 873, 1107, 1311
MspR9I	CCNGG	3 cut(s)	556, 742, 1107
Mva1269I	GAATGC	2 cut(s)	509, 796
MvaI	CCWGG	1 cut(s)	742
MvnI	CGCG	4 cut(s)	360, 410, 415, 1373
MwoI	GCNNNNNNNGC	7 cut(s)	178, 197, 501, 1173, 1182, 1281, 1404
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	2 cut(s)	556, 1107
NcoI	CCATGG	2 cut(s)	910, 1059
NdeII	GATC	5 cut(s)	14, 48, 228, 310, 753
NgoMIV	GCCGGC	1 cut(s)	22
NlaIII	CATG	6 cut(s)	79, 637, 720, 914, 978, 1063
NlaIV	GGNNCC	6 cut(s)	6, 224, 349, 560, 1065, 1246
NmuCI	GTSAC	4 cut(s)	550, 719, 916, 1148
NsiI	ATGCAT	1 cut(s)	796
NspI	RCATGY	2 cut(s)	79, 720
PaqCI	CACCTGC	1 cut(s)	1259
PciI	ACATGT	2 cut(s)	75, 716
PctI	GAATGC	2 cut(s)	509, 796
PdiI	GCCGGC	1 cut(s)	24
PdmI	GAANNNNTTC	2 cut(s)	302, 771
PfeI	GAWTC	2 cut(s)	425, 1093
PflMI	CCANNNNNTGG	1 cut(s)	447
PinAI	ACCGGT	2 cut(s)	300, 402
Ple19I	CGATCG	2 cut(s)	17, 756
PleI	GAGTC	1 cut(s)	431
PmaCI	CACGTG	1 cut(s)	1182
PmlI	CACGTG	1 cut(s)	1182
PpsI	GAGTC	1 cut(s)	431
Ppu21I	YACGTR	1 cut(s)	1182
PpuMI	RGGWCCY	1 cut(s)	559
PscI	ACATGT	2 cut(s)	75, 716
Psp5II	RGGWCCY	1 cut(s)	559
Psp6I	CCWGG	1 cut(s)	740
PspCI	CACGTG	1 cut(s)	1182
PspFI	CCCAGC	2 cut(s)	504, 987
PspGI	CCWGG	1 cut(s)	740
PspN4I	GGNNCC	6 cut(s)	6, 224, 349, 560, 1065, 1246
PspPI	GGNCC	7 cut(s)	5, 34, 67, 135, 559, 907, 1109
PspPPI	RGGWCCY	1 cut(s)	559
PsrI	GAACNNNNNNTAC	4 cut(s)	266, 298, 311, 343
PstNI	CAGNNNCTG	2 cut(s)	586, 1275
PsuI	RGATCY	1 cut(s)	228
PvuI	CGATCG	2 cut(s)	17, 756
PvuII	CAGCTG	2 cut(s)	129, 492
RsaI	GTAC	4 cut(s)	11, 251, 329, 407
RsaNI	GTAC	4 cut(s)	10, 250, 328, 406
RseI	CAYNNNNRTG	1 cut(s)	1010
SaqAI	TTAA	3 cut(s)	234, 468, 1417
Sau3AI	GATC	5 cut(s)	14, 48, 228, 310, 753
Sau96I	GGNCC	7 cut(s)	5, 34, 67, 135, 559, 907, 1109
SchI	GAGTC	1 cut(s)	431
ScrFI	CCNGG	3 cut(s)	556, 742, 1107
SduI	GDGCHC	1 cut(s)	183
SfaNI	GCATC	4 cut(s)	608, 700, 1002, 1200
SfcI	CTRYAG	1 cut(s)	727
SinI	GGWCC	5 cut(s)	34, 135, 559, 907, 1109
SmiMI	CAYNNNNRTG	1 cut(s)	1010
Sse9I	AATT	2 cut(s)	672, 1053
SspMI	CTAG	1 cut(s)	548
StyD4I	CCNGG	3 cut(s)	554, 740, 1105
StyI	CCWWGG	6 cut(s)	201, 343, 484, 910, 1059, 1341
TaaI	ACNGT	4 cut(s)	58, 785, 922, 1271
TaiI	ACGT	2 cut(s)	892, 1184
TaqI	TCGA	8 cut(s)	72, 155, 255, 269, 317, 648, 752, 756
TaqII	GACCGA	2 cut(s)	152, 281
TasI	AATT	2 cut(s)	672, 1053
TauI	GCSGC	2 cut(s)	220, 415
TfiI	GAWTC	2 cut(s)	425, 1093
Tru1I	TTAA	3 cut(s)	234, 468, 1417
Tru9I	TTAA	3 cut(s)	234, 468, 1417
TscAI	CASTG	4 cut(s)	61, 790, 1388, 1417
TseFI	GTSAC	4 cut(s)	550, 719, 916, 1148
TseI	GCWGC	8 cut(s)	126, 489, 492, 1015, 1185, 1188, 1275, 1407
Tsp45I	GTSAC	4 cut(s)	550, 719, 916, 1148
TspDTI	ATGAA	3 cut(s)	627, 643, 780
TspRI	CASTG	4 cut(s)	61, 790, 1388, 1417
Van91I	CCANNNNNTGG	1 cut(s)	447
VpaK11BI	GGWCC	5 cut(s)	34, 135, 559, 907, 1109
XagI	CCTNNNNNAGG	1 cut(s)	699
XceI	RCATGY	2 cut(s)	79, 720
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1388
XmiI	GTMKAC	1 cut(s)	591
XmnI	GAANNNNTTC	2 cut(s)	302, 771
XspI	CTAG	1 cut(s)	548
Zsp2I	ATGCAT	1 cut(s)	796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.