AT5G43210

GIY-YIG catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009332
Physical Location & Seq
Forward (+)
17347565 .. 17348746
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43210.1

Sequence Viewer

Length: 513 bp
ATGACGAGGTTGCTCTCTAGGACGTTCCCGTCCGTGAAGCTTCGCGATTCCAATCCTAAAGCTCCCGAATTCAAAAATTTGAAAAATTCACAAACCCAAGTTCCGATTCCATCGTCTTCTTCTCCGCAAGAGAACTCAAAGATTCCCAAATCCAAATCCTGGTCTGTTTATCTCATTCTCTCCACGACCGAACCCATCAAAACATATGTCGGAATCACCACCGATTTTTCTCGCCGATTAAAGCAGCACAATGGTGAAATCAGAGGTGGTGCAAAAGCTTCAAGTGCAGGAAGACCATGGCTTTGTGCTTGCATTATCACTGGATTCACTTGTTTAAGTCAAGCTTCTTCGTTTGAATCAAAATGGAAGATCTTTACAAGAAAGTTACCGCGGAGAAAGAAAGACGAAGACATGAGTCAGAGTGATGCTTTGCTACAACACCGTAGGAGAGCTTTAAACAAAGTCGAGGAATCATTAGACTGTAGTTATCTTGAAACTGACTGGAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

170

Amino Acids

19.35

Weight (kDa)

9.94

Isoelectric Point (pI)

65.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 54 - 127 1.4e-12 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 28
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 2 cut(s) 45, 391
AciI CCGC 3 cut(s) 125, 389, 391
AcsI RAATTY 3 cut(s) 68, 76, 85
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 5 cut(s) 73, 82, 282, 356, 494
AjnI CCWGG 1 cut(s) 158
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AleI CACNNNNGTG 1 cut(s) 252
AluBI AGCT 5 cut(s) 40, 62, 278, 344, 452
AluI AGCT 5 cut(s) 40, 62, 278, 344, 452
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 3 cut(s) 68, 76, 85
ArsI GACNNNNNNTTYG 4 cut(s) 192, 224, 285, 317
AsuHPI GGTGA 2 cut(s) 208, 266
BbsI GAAGAC 3 cut(s) 108, 298, 414
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 118, 203
BciT130I CCWGG 1 cut(s) 160
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 481
BglII AGATCT 1 cut(s) 369
BisI GCNGC 1 cut(s) 245
BlsI GCNGC 1 cut(s) 246
Bme1390I CCNGG 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 415
BoxI GACNNNNGTC 1 cut(s) 414
BpiI GAAGAC 3 cut(s) 108, 298, 414
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 296, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse1I ACTGG 2 cut(s) 325, 506
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 2 cut(s) 296, 389
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 325, 506
BseXI GCAGC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 306
Bsh1236I CGCG 2 cut(s) 45, 391
Bsh1285I CGRYCG 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 159
Bsp143I GATC 1 cut(s) 369
Bsp19I CCATGG 1 cut(s) 296
Bsp68I TCGCGA 1 cut(s) 45
BspACI CCGC 3 cut(s) 125, 389, 391
BspFNI CGCG 2 cut(s) 45, 391
BsrI ACTGG 2 cut(s) 325, 506
BssECI CCNNGG 2 cut(s) 296, 389
BssMI GATC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 2 cut(s) 443, 482
BstC8I GCNNGC 1 cut(s) 310
BstDSI CCRYGG 2 cut(s) 296, 389
BstFNI CGCG 2 cut(s) 45, 391
BstKTI GATC 1 cut(s) 372
BstMBI GATC 1 cut(s) 369
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 1 cut(s) 160
BstPAI GACNNNNGTC 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 481
BstUI CGCG 2 cut(s) 45, 391
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 3 cut(s) 108, 298, 414
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 296, 389
BtsIMutI CAGTG 1 cut(s) 318
BtuMI TCGCGA 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 310
Cfr42I CCGCGG 1 cut(s) 392
CviAII CATG 2 cut(s) 297, 412
CviJI RGCY 6 cut(s) 40, 62, 278, 301, 344, 452
CviKI_1 RGCY 6 cut(s) 40, 62, 278, 301, 344, 452
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 2 cut(s) 300, 415
FaiI YATR 4 cut(s) 205, 207, 298, 413
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 2 cut(s) 296, 411
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 245
Fsp4HI GCNGC 1 cut(s) 245
FspBI CTAG 1 cut(s) 18
GluI GCNGC 1 cut(s) 245
Hin1II CATG 2 cut(s) 300, 415
HindIII AAGCTT 3 cut(s) 38, 276, 342
HinfI GANTC 8 cut(s) 47, 106, 142, 213, 324, 356, 415, 470
HphI GGTGA 2 cut(s) 208, 266
Hpy188I TCNGA 4 cut(s) 105, 212, 263, 420
Hpy188III TCNNGA 3 cut(s) 44, 65, 491
HpyCH4III ACNGT 2 cut(s) 443, 482
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 272, 287, 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 300, 415
KspI CCGCGG 1 cut(s) 392
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 5 cut(s) 145, 172, 273, 306, 487
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 415
MaeI CTAG 1 cut(s) 18
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 384
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 6 cut(s) 108, 111, 303, 339, 379, 419
MflI RGATCY 1 cut(s) 369
MluCI AATT 3 cut(s) 68, 76, 85
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 2 cut(s) 257, 460
MseI TTAA 3 cut(s) 239, 335, 455
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 391
MspR9I CCNGG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 160
MvnI CGCG 2 cut(s) 45, 391
MwoI GCNNNNNNNGC 1 cut(s) 284
NcoI CCATGG 1 cut(s) 296
NdeI CATATG 1 cut(s) 205
NdeII GATC 1 cut(s) 369
NlaIII CATG 2 cut(s) 300, 415
NruI TCGCGA 1 cut(s) 45
OliI CACNNNNGTG 1 cut(s) 252
PfeI GAWTC 7 cut(s) 47, 106, 142, 213, 324, 356, 470
PflMI CCANNNNNTGG 1 cut(s) 159
PkrI GCNGC 1 cut(s) 246
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
PshAI GACNNNNGTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 158
PspGI CCWGG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 369
RruI TCGCGA 1 cut(s) 45
RseI CAYNNNNRTG 1 cut(s) 252
SacII CCGCGG 1 cut(s) 392
SaqAI TTAA 3 cut(s) 239, 335, 455
SatI GCNGC 1 cut(s) 245
Sau3AI GATC 1 cut(s) 369
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 160
SetI ASST 8 cut(s) 11, 26, 42, 64, 268, 280, 346, 454
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 481
Sfr303I CCGCGG 1 cut(s) 392
SgrBI CCGCGG 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 252
Sse9I AATT 3 cut(s) 68, 76, 85
SsiI CCGC 3 cut(s) 125, 389, 391
SspMI CTAG 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 443, 482
TaiI ACGT 1 cut(s) 26
TaqI TCGA 1 cut(s) 465
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 68, 76, 85
TfiI GAWTC 7 cut(s) 47, 106, 142, 213, 324, 356, 470
Tru1I TTAA 3 cut(s) 239, 335, 455
Tru9I TTAA 3 cut(s) 239, 335, 455
TscAI CASTG 1 cut(s) 325
TseI GCWGC 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 159
XapI RAATTY 3 cut(s) 68, 76, 85
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.