Rroxscaffold_4G00329010

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009332
Physical Location & Seq
Forward (+)
62150085 .. 62153522
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329010.1

Sequence Viewer

Length: 531 bp
ATGATGACAAGACTCTCCAGAACATTCCCTTCCGTAAAGCTCCCAAACCCTCACCCCTCCTCAATTCTATTACCATGTTCAACATCACTACCCAATATCAAATCAGAAGCAAAATCGAATTCTCCCTCAAAATCAAAACCAAAATCGAGGTCTTGGTCTGTGTACCTCATCCTTTCCACCAATGCACCCATCAAGACCTACGTTGGTGTCACCACTGATTTCTCTCGCCGTCTGAAACAACACAATGGTGAACTAAAAGGTGGTGCAAAAGCTTCCCGTGCTGGAAGACCATGGATTTGTGCGTGCCTAATACATGGTTTCAAGGACCAAAATGAAGCATGTTCATTTGAATCAAAATGGAAAATTTTATCAAGGAGTTTGCCTCGCAAAAGAAAGGATGACAACAAGGTTGAGCAAGTAAATGATTTGTCGCTTCCTCTACTACAACACAGGCAAGCAGCTCTAAATAGAGTTGAAGGTATATTAGATTGCGCTCACTTAGAAATTGACTGGAAACTGAACCCCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

176

Amino Acids

19.77

Weight (kDa)

9.99

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 52 - 126 4.1e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 529
AcsI RAATTY 2 cut(s) 118, 363
AfaI GTAC 1 cut(s) 164
AgsI TTSAA 4 cut(s) 81, 322, 350, 476
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 3 cut(s) 40, 272, 461
AluI AGCT 3 cut(s) 40, 272, 461
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 2 cut(s) 118, 363
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 279, 311
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 3 cut(s) 44, 202, 260
AvaII GGWCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 470
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 213
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BsaJI CCNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 168, 403
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 470
Bsp19I CCATGG 1 cut(s) 290
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 304, 456
BstDEI CTNAG 1 cut(s) 499
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 168, 403
BstHHI GCGC 1 cut(s) 494
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 470
BstV2I GAAGAC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 2 cut(s) 168, 403
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 304, 456
CfoI GCGC 1 cut(s) 494
Cfr13I GGNCC 1 cut(s) 325
Csp6I GTAC 1 cut(s) 163
CviAII CATG 4 cut(s) 75, 291, 314, 339
CviJI RGCY 3 cut(s) 40, 272, 461
CviKI_1 RGCY 3 cut(s) 40, 272, 461
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 4 cut(s) 78, 294, 317, 342
FaiI YATR 6 cut(s) 76, 292, 315, 340, 482, 529
FatI CATG 4 cut(s) 74, 290, 313, 338
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 2 cut(s) 155, 410
Fsp4HI GCNGC 1 cut(s) 459
GlaI GCGC 1 cut(s) 493
GluI GCNGC 1 cut(s) 459
HhaI GCGC 1 cut(s) 494
Hin1II CATG 4 cut(s) 78, 294, 317, 342
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 2 cut(s) 12, 350
HphI GGTGA 3 cut(s) 44, 202, 260
Hpy166II GTNNAC 2 cut(s) 163, 251
Hpy188I TCNGA 2 cut(s) 106, 234
Hpy188III TCNNGA 2 cut(s) 18, 193
Hpy8I GTNNAC 2 cut(s) 163, 251
HpyAV CCTTC 2 cut(s) 39, 470
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 185, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 4 cut(s) 78, 294, 317, 342
HspAI GCGC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 45
LpnPI CCDG 4 cut(s) 31, 267, 436, 496
Lsp1109I GCAGC 1 cut(s) 470
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 1 cut(s) 297
MluCI AATT 4 cut(s) 63, 118, 363, 504
MlyI GAGTC 1 cut(s) 6
MnlI CCTC 8 cut(s) 60, 67, 70, 136, 141, 176, 393, 447
MslI CAYNNNNRTG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 278
NcoI CCATGG 1 cut(s) 290
NlaIII CATG 4 cut(s) 78, 294, 317, 342
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 246
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PsiI TTATAA 1 cut(s) 529
PspPI GGNCC 1 cut(s) 325
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 246
SatI GCNGC 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 1 cut(s) 6
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 246
Sse9I AATT 4 cut(s) 63, 118, 363, 504
StyI CCWWGG 1 cut(s) 290
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 116, 146
TasI AATT 4 cut(s) 63, 118, 363, 504
TfiI GAWTC 1 cut(s) 350
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 333, 348
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 2 cut(s) 118, 363
XceI RCATGY 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.