Rh1DG036000

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0009332
Physical Location & Seq
Forward (+)
5799466 .. 5804698
5233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG036000.1

Sequence Viewer

Length: 558 bp
ATGGAGAAGCAGGAGAGGAAGGCGGAGGTGGACTCGAAGGAAGGGGAGGGGTCGAAGGAATTGGCGGCCGAGCAACACGACGCCGACTCTGGAGGCGGAGAGGGTGTCGGTGGAAAGGCGAAGAGCACTGGATGGGAGGAGCGTGGGGAGGATGGAGCTGGAGGAGATAGATCGGAGTCTTGGTCTGTGTATCTCATCCTTTCCACCAATGCACCCATCAAGACCTACGTTGGTGTCACCACTGATTTCTCTCGCCGTCTGAAACAACACAATGGTGAACTAAAAGGTGGGGCAAAGGCATCCCATGCTGGAAGACCATGGATTTGTGCGTGCCTAATACATGGTTTCAAGGACCAAAATGAAGCATGTGCATTTGAATCAAAATGGAAAACTTTATCAAGGAGTTTGCCTCGCAAAAGAAAGGACGATAACAAGGTAGAGCAAGTAAGTGATTTGTCGCTTCCTCTACTACAACATAGGCAAGCAGCTCTAAATAGAGTTAAAGGTATATTTGATTGTACTCACTTAGAAATTGACTGGAAACTGAACCCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

185

Amino Acids

20.4

Weight (kDa)

7.01

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 61 - 135 1.9e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 556
AciI CCGC 3 cut(s) 23, 65, 96
AcoI YGGCCR 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 520
AgsI TTSAA 2 cut(s) 349, 377
AleI CACNNNNGTG 1 cut(s) 273
AluBI AGCT 2 cut(s) 158, 488
AluI AGCT 2 cut(s) 158, 488
Alw21I GWGCWC 1 cut(s) 128
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 485
ArsI GACNNNNNNTTYG 2 cut(s) 306, 338
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 229, 287
AvaII GGWCC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 497
BccI CCATC 3 cut(s) 126, 146, 224
BceAI ACGGC 1 cut(s) 240
BisI GCNGC 2 cut(s) 66, 486
BlsI GCNGC 2 cut(s) 67, 487
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 352
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 394, 426
BpmI CTGGAG 2 cut(s) 111, 180
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 317
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 167, 197
Bse1I ACTGG 2 cut(s) 133, 542
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 4 cut(s) 137, 157, 195, 299
BseNI ACTGG 2 cut(s) 133, 542
BseRI GAGGAG 2 cut(s) 152, 177
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 497
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 128
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 170
Bsp19I CCATGG 1 cut(s) 317
BspACI CCGC 3 cut(s) 23, 65, 96
BspANI GGCC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 116
BsrI ACTGG 2 cut(s) 133, 542
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 1 cut(s) 170
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 331, 483
BstDEI CTNAG 1 cut(s) 526
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 4 cut(s) 137, 157, 195, 299
BstKTI GATC 1 cut(s) 173
BstMBI GATC 1 cut(s) 170
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 497
BstV2I GAAGAC 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 4 cut(s) 137, 157, 195, 299
BtsIMutI CAGTG 2 cut(s) 126, 240
Cac8I GCNNGC 2 cut(s) 331, 483
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 519
CviAII CATG 4 cut(s) 305, 318, 341, 366
CviJI RGCY 3 cut(s) 68, 158, 488
CviKI_1 RGCY 3 cut(s) 68, 158, 488
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 1 cut(s) 526
DpnI GATC 1 cut(s) 172
DpnII GATC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 2 cut(s) 38, 111
EclXI CGGCCG 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 4 cut(s) 308, 321, 344, 369
FaiI YATR 7 cut(s) 306, 319, 342, 367, 477, 509, 556
FatI CATG 4 cut(s) 304, 317, 340, 365
Fnu4HI GCNGC 2 cut(s) 66, 486
FokI GGATG 4 cut(s) 144, 164, 182, 286
Fsp4HI GCNGC 2 cut(s) 66, 486
GluI GCNGC 2 cut(s) 66, 486
GsuI CTGGAG 2 cut(s) 111, 180
HaeIII GGCC 1 cut(s) 68
HgaI GACGC 1 cut(s) 89
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 308, 321, 344, 369
HinfI GANTC 4 cut(s) 32, 86, 176, 377
HphI GGTGA 2 cut(s) 229, 287
Hpy166II GTNNAC 2 cut(s) 31, 278
Hpy188I TCNGA 2 cut(s) 175, 261
Hpy188III TCNNGA 2 cut(s) 90, 220
Hpy8I GTNNAC 2 cut(s) 31, 278
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 13, 31, 35, 49
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 212, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 1 cut(s) 526
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 308, 321, 344, 369
Kzo9I GATC 1 cut(s) 170
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 139, 155
LpnPI CCDG 5 cut(s) 75, 114, 144, 294, 523
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 308
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 172
MboI GATC 1 cut(s) 170
MboII GAAGA 2 cut(s) 133, 324
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 2 cut(s) 59, 531
MlyI GAGTC 3 cut(s) 26, 80, 185
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 305
NcoI CCATGG 1 cut(s) 317
NdeII GATC 1 cut(s) 170
NlaIII CATG 4 cut(s) 308, 321, 344, 369
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 235
NspI RCATGY 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 116
PfeI GAWTC 1 cut(s) 377
PkrI GCNGC 2 cut(s) 67, 487
PleI GAGTC 3 cut(s) 26, 80, 184
PpsI GAGTC 3 cut(s) 26, 80, 184
PsiI TTATAA 1 cut(s) 556
PspPI GGNCC 1 cut(s) 352
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
RseI CAYNNNNRTG 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 2 cut(s) 66, 486
Sau3AI GATC 1 cut(s) 170
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 3 cut(s) 26, 80, 185
SduI GDGCHC 1 cut(s) 128
SetI ASST 8 cut(s) 30, 160, 227, 231, 289, 438, 490, 508
SfaNI GCATC 1 cut(s) 308
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 2 cut(s) 59, 531
SsiI CCGC 3 cut(s) 23, 65, 96
StyI CCWWGG 1 cut(s) 317
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 35, 53
TasI AATT 2 cut(s) 59, 531
TatI WGTACW 1 cut(s) 518
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 133, 247
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 375
TspRI CASTG 2 cut(s) 133, 247
VpaK11BI GGWCC 1 cut(s) 352
XceI RCATGY 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.