Rh1CG034400

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0009332
Physical Location & Seq
Reverse (-)
6558278 .. 6561098
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG034400.1

Sequence Viewer

Length: 531 bp
ATGATGACACGACTCTCCAGAACATTCCCTTCCGTAAAGCTCCCAAACCCTCACCCCTCCTCAATTCTATTACCATGTTCAACATCACTACCCAATATCAAATCAGAAGCAAAATCGAATTCTCCCTCAAAATCAAAACCAAAATCGAGGTCTTGGTCTGTGTATCTCATCCTTTCCACCAATGCACCCATCAAGACCTACGTTGGTGTCACCACCGATTTCTCTCGCCGTCTGAAACAACACAATGGTGAACTAAAAGGTGGGGCAAAGGCATCCCATGCTGGAAGACCGTGGATTTGTGCGTGCCTAATACATGGTTTCAAGGACCAAAATGAAGCATGTGCATTTGAATCAAAATGGAAAACTTTATCAAGGAGTTTGCCTCGCAAAAGAAAGGATGATAACAAGGTAGAGCAAGTAAATGATTTGTCGCGTCCTCTACTACAACATAGGCAAGCAGCTCTAAATAGAGTTAAAGGTAAATTTGATTGTACTCACTTAGAAATTGACTGGAAACTGAATCCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

176

Amino Acids

19.84

Weight (kDa)

10.14

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 52 - 126 1.7e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 529
AccII CGCG 1 cut(s) 433
AcsI RAATTY 2 cut(s) 118, 482
AfaI GTAC 1 cut(s) 493
AgsI TTSAA 3 cut(s) 81, 322, 350
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 2 cut(s) 40, 461
AluI AGCT 2 cut(s) 40, 461
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 2 cut(s) 118, 482
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 279, 311
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 3 cut(s) 44, 202, 260
AvaII GGWCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 470
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 213
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmsI GCATC 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BsaJI CCNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 3 cut(s) 168, 272, 403
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 433
BspFNI CGCG 1 cut(s) 433
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 304, 456
BstDEI CTNAG 1 cut(s) 499
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 3 cut(s) 168, 272, 403
BstFNI CGCG 1 cut(s) 433
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 342
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 470
BstV2I GAAGAC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 3 cut(s) 168, 272, 403
Cac8I GCNNGC 2 cut(s) 304, 456
Cfr13I GGNCC 1 cut(s) 325
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 492
CviAII CATG 4 cut(s) 75, 278, 314, 339
CviJI RGCY 2 cut(s) 40, 461
CviKI_1 RGCY 2 cut(s) 40, 461
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 118
FaeI CATG 4 cut(s) 78, 281, 317, 342
FaiI YATR 6 cut(s) 76, 279, 315, 340, 450, 529
FatI CATG 4 cut(s) 74, 277, 313, 338
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 3 cut(s) 155, 259, 410
Fsp4HI GCNGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 459
HgaI GACGC 1 cut(s) 422
Hin1II CATG 4 cut(s) 78, 281, 317, 342
HinfI GANTC 3 cut(s) 12, 350, 520
HphI GGTGA 3 cut(s) 44, 202, 260
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 2 cut(s) 106, 234
Hpy188III TCNNGA 2 cut(s) 18, 193
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 185, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 4 cut(s) 78, 281, 317, 342
LmnI GCTCC 1 cut(s) 45
LpnPI CCDG 3 cut(s) 31, 267, 496
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 1 cut(s) 281
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 1 cut(s) 297
MluCI AATT 4 cut(s) 63, 118, 482, 504
MlyI GAGTC 1 cut(s) 6
MnlI CCTC 7 cut(s) 60, 67, 70, 136, 141, 393, 447
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 246
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 1 cut(s) 278
NlaIII CATG 4 cut(s) 78, 281, 317, 342
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 246
PfeI GAWTC 2 cut(s) 350, 520
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PsiI TTATAA 1 cut(s) 529
PspPI GGNCC 1 cut(s) 325
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 1 cut(s) 6
SetI ASST 8 cut(s) 42, 152, 200, 204, 262, 411, 463, 481
SfaNI GCATC 1 cut(s) 281
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 246
Sse9I AATT 4 cut(s) 63, 118, 482, 504
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 116, 146
TasI AATT 4 cut(s) 63, 118, 482, 504
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 2 cut(s) 350, 520
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 2 cut(s) 118, 482
XceI RCATGY 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.