FvH4_7g01930

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009332
Physical Location & Seq
Reverse (-)
2309507 .. 2312692
3186 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01930.t1

Sequence Viewer

Length: 525 bp
ATGATGACGCGGCTCTCCACGACATTTCCTTCCGTGAAGCTCACAAACCCTCGCCCCTCCTCAAATCTATTATCATGTTCAACATCACTACCCAAGATCAAATCAGAAGCAACATCCAATTCTCCCTCAAAATCAAAGTCAAGGTCTTGGTCTGTGTATCTCATCCTTTCCACCAATGCCCCCATCAAGACCTACGTTGGTGTCACCACCGATTTCTCTCGCCGTCTGAAACAACACAATGGTGAACTAAAAGGTGGCGCAAAAGCATCCCGTGCTGGAAGACCATGGATTTGTGCATGCCTAGTACATGGTTTCAAGGACCAAAACGAAGTGTGTTCTTTTGAATCAAAATGGAAAACTTTATCAAGGAGTTTGCCTCGAAAAAGAAAGGATGACAGTGAGGCAGAACAAGCAAACAATTTGTCGCTTCCTCTACTACAACACAGGCAAGCAGCTCTAAATAGAGTTAAAGGTATATTTGATTGCACTGACTTGGAAATTGACTGGAAACTGAACCCGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

175

Amino Acids

19.45

Weight (kDa)

9.94

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 50 - 124 3.5e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 523
AccII CGCG 1 cut(s) 10
AciI CCGC 1 cut(s) 10
AfaI GTAC 1 cut(s) 306
AgsI TTSAA 3 cut(s) 81, 316, 344
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 2 cut(s) 40, 455
AluI AGCT 2 cut(s) 40, 455
ApeKI GCWGC 1 cut(s) 452
ArsI GACNNNNNNTTYG 8 cut(s) 122, 128, 154, 160, 273, 305, 407, 439
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 2 cut(s) 196, 254
AvaII GGWCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 464
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 207
BfaI CTAG 1 cut(s) 302
BisI GCNGC 2 cut(s) 11, 453
BlsI GCNGC 2 cut(s) 12, 454
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 319
BmsI GCATC 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 286
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BsaJI CCNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 4 cut(s) 113, 162, 266, 397
BseNI ACTGG 1 cut(s) 509
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 10
Bsp143I GATC 1 cut(s) 96
Bsp19I CCATGG 1 cut(s) 284
BspACI CCGC 1 cut(s) 10
BspFNI CGCG 1 cut(s) 10
BsrI ACTGG 1 cut(s) 509
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 298, 450
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 4 cut(s) 113, 162, 266, 397
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 2 cut(s) 272, 410
BstNSI RCATGY 1 cut(s) 300
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 286
BtgI CCRYGG 1 cut(s) 284
BtsCI GGATG 4 cut(s) 113, 162, 266, 397
BtsIMutI CAGTG 2 cut(s) 403, 486
Cac8I GCNNGC 2 cut(s) 298, 450
CfoI GCGC 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 305
CviAII CATG 4 cut(s) 75, 285, 297, 308
CviJI RGCY 3 cut(s) 13, 40, 455
CviKI_1 RGCY 3 cut(s) 13, 40, 455
CviQI GTAC 1 cut(s) 305
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 284
FaeI CATG 4 cut(s) 78, 288, 300, 311
FaiI YATR 6 cut(s) 76, 286, 298, 309, 476, 523
FatI CATG 4 cut(s) 74, 284, 296, 307
Fnu4HI GCNGC 2 cut(s) 11, 453
FokI GGATG 4 cut(s) 100, 149, 253, 404
Fsp4HI GCNGC 2 cut(s) 11, 453
FspBI CTAG 1 cut(s) 302
GlaI GCGC 1 cut(s) 259
GluI GCNGC 2 cut(s) 11, 453
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 260
Hin1II CATG 4 cut(s) 78, 288, 300, 311
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HinfI GANTC 1 cut(s) 344
HphI GGTGA 2 cut(s) 196, 254
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 106, 228
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 296, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 410
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 4 cut(s) 78, 288, 300, 311
HspAI GCGC 1 cut(s) 258
Kzo9I GATC 1 cut(s) 96
LpnPI CCDG 3 cut(s) 261, 430, 490
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 1 cut(s) 275
MaeI CTAG 1 cut(s) 302
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 1 cut(s) 291
MluCI AATT 3 cut(s) 118, 418, 498
MnlI CCTC 7 cut(s) 60, 67, 70, 136, 387, 394, 441
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 240
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 2 cut(s) 272, 410
NcoI CCATGG 1 cut(s) 284
NdeII GATC 1 cut(s) 96
NlaIII CATG 4 cut(s) 78, 288, 300, 311
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 240
PaeI GCATGC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 2 cut(s) 12, 454
PsiI TTATAA 1 cut(s) 523
PspPI GGNCC 1 cut(s) 319
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 240
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 2 cut(s) 11, 453
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 319
SetI ASST 7 cut(s) 42, 146, 194, 198, 256, 457, 475
SfaNI GCATC 1 cut(s) 275
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 240
SphI GCATGC 1 cut(s) 300
Sse9I AATT 3 cut(s) 118, 418, 498
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 379
TasI AATT 3 cut(s) 118, 418, 498
TatI WGTACW 1 cut(s) 304
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 2 cut(s) 403, 493
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 2 cut(s) 403, 493
VpaK11BI GGWCC 1 cut(s) 319
XceI RCATGY 1 cut(s) 300
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.