Rorug01G0022400

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009332
Physical Location & Seq
Reverse (-)
3774442 .. 3778095
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0022400.1

Sequence Viewer

Length: 969 bp
ATGAGTGGAGCAGGGAAGGTGGCGGTGAGCGTCACCGGAGCATCCGGTTACATTGCTTCATGGCTGGTCAAACTGTTGCTGCAACGCGGCTACACTGTCAAGGCCTCTGTTCGTGACCCAAATGACAAGAAGAAAACAGAACACTTGCTTGCACTAGATGGAGCCAAGGAAAGGCTTCAATTGTTCAAAGCAGACCTGCTGGAAGAAGGATCTTTTGATTCCTTAGTTGATGGTAGTGTAGCTGTTTTTCATACAGCATCCCCATTTTATCACAATCCCAATGATCCACAGGTAGAACTAATTGACCCTGCCTTGAAGGGAACGCTTAATGTCCTCAGATCATGTGTTAAGGTTCCGTCTATCAAGAGGGTGGTTATAACTTCCTCTATGGCAGCAGTCGCGTTCAATGGGAAACCTGTCGCTCCTGATGTAATAATTGATGAATCTTGGTTTTCAGATCCAGCTTTCTGTGAAAAATCAAAGGCTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTGTTACAATAAATCCGGGATTGGTGATTGGCCCTCTCTTACAGCCAACTCTCAACACAAGTGTGGAGCCAATCCTGAAACTCATAAATGGGACTGAAAAATTTCCAAACACAACTTACAGATTTGTTGATGTTAGAGATGTTGCCAATGCACATATTCTAGCCTTTGAGAATCCTTCAGCTAGTGGTCGCTATTGTTTAGTTGGAAGTGTAACACACTGTTCAGAGATTGTGAAAATTCTGCGTCATATCTTCCCTGCTCTCAGTCTCCCAGAAAAGCCTGCAGATGACAAACCTTTCACACCAACTTACCAGGTATCCAAGGAAAGAGCACAAACTTTGGGTGTAAAGTATACTCCGCTAGAAGTGACTCTAAAGGATACTGTTGAAAGTTTGAAGGACAAGAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

322

Amino Acids

35.26

Weight (kDa)

8.2

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 105 2.6e-10 NmrA-like family
Epimerase PF01370 10 - 247 1.8e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 242 1.1e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 200 1.9e-11 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 251 3e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 133 3.3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 908
AccII CGCG 2 cut(s) 87, 401
AciI CCGC 3 cut(s) 23, 87, 914
AclWI GGATC 3 cut(s) 217, 278, 452
AcsI RAATTY 2 cut(s) 656, 792
AcuI CTGAAG 1 cut(s) 717
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 7 cut(s) 179, 187, 316, 406, 501, 944, 952
AjnI CCWGG 1 cut(s) 867
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 5 cut(s) 242, 464, 512, 521, 737
AluI AGCT 5 cut(s) 242, 464, 512, 521, 737
Alw21I GWGCWC 1 cut(s) 889
Alw26I GTCTC 1 cut(s) 827
AlwI GGATC 3 cut(s) 217, 278, 452
AoxI GGCC 2 cut(s) 102, 586
ApeKI GCWGC 3 cut(s) 79, 392, 521
ApoI RAATTY 2 cut(s) 656, 792
Asp700I GAANNNNTTC 2 cut(s) 174, 657
AspS9I GGNCC 1 cut(s) 587
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 25, 37, 592
Bbv12I GWGCWC 1 cut(s) 889
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 66, 404, 508
BccI CCATC 2 cut(s) 152, 224
BciT130I CCWGG 1 cut(s) 869
BciVI GTATCC 2 cut(s) 883, 928
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 827
BfaI CTAG 4 cut(s) 155, 716, 738, 917
BfmI CTRYAG 1 cut(s) 837
BfuAI ACCTGC 1 cut(s) 204
BfuI GTATCC 2 cut(s) 883, 928
BisI GCNGC 4 cut(s) 80, 88, 393, 522
BlsI GCNGC 4 cut(s) 81, 89, 394, 523
Bme1390I CCNGG 2 cut(s) 573, 869
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 3 cut(s) 163, 354, 624
BmrFI CCNGG 2 cut(s) 573, 869
BmsI GCATC 2 cut(s) 50, 266
Bpu10I CCTNAGC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 165, 876
BsaWI WCCGGW 2 cut(s) 35, 44
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 869
BseDI CCNNGG 2 cut(s) 165, 876
BseGI GGATG 2 cut(s) 41, 257
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 3 cut(s) 349, 504, 832
BseXI GCAGC 3 cut(s) 66, 404, 508
Bsh1236I CGCG 2 cut(s) 87, 401
BshFI GGCC 2 cut(s) 104, 588
BsiHKAI GWGCWC 1 cut(s) 889
BsiSI CCGG 3 cut(s) 36, 45, 572
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 827
BsmFI GGGAC 1 cut(s) 661
BsnI GGCC 2 cut(s) 104, 588
Bsp1286I GDGCHC 1 cut(s) 889
Bsp143I GATC 4 cut(s) 209, 283, 338, 457
BspACI CCGC 3 cut(s) 23, 87, 914
BspANI GGCC 2 cut(s) 104, 588
BspCNI CTCAG 3 cut(s) 348, 505, 831
BspFNI CGCG 2 cut(s) 87, 401
BspLI GGNNCC 3 cut(s) 163, 354, 624
BspMAI CTGCAG 1 cut(s) 841
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 3 cut(s) 217, 278, 452
BsrDI GCAATG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 165, 876
BssMI GATC 4 cut(s) 209, 283, 338, 457
BssNAI GTATAC 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 165, 876
Bst1107I GTATAC 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 4 cut(s) 75, 97, 776, 940
BstC8I GCNNGC 2 cut(s) 150, 837
BstDEI CTNAG 4 cut(s) 223, 335, 513, 818
BstF5I GGATG 2 cut(s) 41, 257
BstFNI CGCG 2 cut(s) 87, 401
BstKTI GATC 4 cut(s) 212, 286, 341, 460
BstMAI GTCTC 1 cut(s) 827
BstMBI GATC 4 cut(s) 209, 283, 338, 457
BstMWI GCNNNNNNNGC 2 cut(s) 398, 518
BstNI CCWGG 1 cut(s) 869
BstSCI CCNGG 2 cut(s) 571, 867
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 2 cut(s) 87, 401
BstV1I GCAGC 3 cut(s) 66, 404, 508
BstX2I RGATCY 2 cut(s) 209, 457
BstYI RGATCY 2 cut(s) 209, 457
BstZ17I GTATAC 1 cut(s) 909
BsuI GTATCC 2 cut(s) 883, 928
BsuRI GGCC 2 cut(s) 104, 588
BtsCI GGATG 2 cut(s) 41, 257
BtsIMutI CAGTG 2 cut(s) 93, 772
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 150, 837
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 2 cut(s) 19, 788
CsiI ACCWGGT 1 cut(s) 867
CviAII CATG 2 cut(s) 60, 342
DdeI CTNAG 4 cut(s) 223, 335, 513, 818
DpnI GATC 4 cut(s) 211, 285, 340, 459
DpnII GATC 4 cut(s) 209, 283, 338, 457
Eco130I CCWWGG 2 cut(s) 165, 876
Eco147I AGGCCT 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 165, 876
ErhI CCWWGG 2 cut(s) 165, 876
FaeI CATG 2 cut(s) 63, 345
FaqI GGGAC 1 cut(s) 661
FatI CATG 2 cut(s) 59, 341
FblI GTMKAC 1 cut(s) 908
Fnu4HI GCNGC 4 cut(s) 80, 88, 393, 522
FokI GGATG 2 cut(s) 28, 244
Fsp4HI GCNGC 4 cut(s) 80, 88, 393, 522
FspBI CTAG 4 cut(s) 155, 716, 738, 917
GluI GCNGC 4 cut(s) 80, 88, 393, 522
HaeIII GGCC 2 cut(s) 104, 588
HapII CCGG 3 cut(s) 36, 45, 572
HgaI GACGC 2 cut(s) 19, 788
Hin1II CATG 2 cut(s) 63, 345
HinfI GANTC 4 cut(s) 218, 443, 727, 925
HpaII CCGG 3 cut(s) 36, 45, 572
HphI GGTGA 3 cut(s) 25, 37, 592
Hpy166II GTNNAC 1 cut(s) 909
Hpy188I TCNGA 3 cut(s) 338, 457, 781
Hpy188III TCNNGA 4 cut(s) 113, 364, 425, 631
Hpy8I GTNNAC 1 cut(s) 909
HpyAV CCTTC 5 cut(s) 10, 200, 310, 741, 946
HpyCH4III ACNGT 4 cut(s) 75, 97, 776, 940
HpyCH4V TGCA 5 cut(s) 82, 152, 536, 707, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 518
HpyF3I CTNAG 4 cut(s) 223, 335, 513, 818
Hsp92II CATG 2 cut(s) 63, 345
Kzo9I GATC 4 cut(s) 209, 283, 338, 457
LmnI GCTCC 5 cut(s) 8, 38, 161, 427, 622
Lsp1109I GCAGC 3 cut(s) 66, 404, 508
LweI GCATC 2 cut(s) 50, 266
MabI ACCWGGT 1 cut(s) 867
MaeI CTAG 4 cut(s) 155, 716, 738, 917
MaeIII GTNAC 6 cut(s) 31, 47, 113, 559, 766, 922
MalI GATC 4 cut(s) 211, 285, 340, 459
MboI GATC 4 cut(s) 209, 283, 338, 457
MboII GAAGA 3 cut(s) 142, 215, 799
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 2 cut(s) 209, 457
MhlI GDGCHC 1 cut(s) 889
MluCI AATT 6 cut(s) 179, 300, 435, 549, 656, 792
MlyI GAGTC 1 cut(s) 919
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 6 cut(s) 115, 344, 360, 394, 508, 600
MroXI GAANNNNTTC 2 cut(s) 174, 657
MseI TTAA 2 cut(s) 327, 348
MslI CAYNNNNRTG 1 cut(s) 617
MspI CCGG 3 cut(s) 36, 45, 572
MspR9I CCNGG 2 cut(s) 573, 869
MunI CAATTG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 869
MvnI CGCG 2 cut(s) 87, 401
MwoI GCNNNNNNNGC 2 cut(s) 398, 518
NciI CCSGG 1 cut(s) 573
NdeII GATC 4 cut(s) 209, 283, 338, 457
NlaIII CATG 2 cut(s) 63, 345
NlaIV GGNNCC 3 cut(s) 163, 354, 624
NmuCI GTSAC 3 cut(s) 31, 113, 922
OliI CACNNNNGTG 1 cut(s) 617
PceI AGGCCT 1 cut(s) 104
PdmI GAANNNNTTC 2 cut(s) 174, 657
PfeI GAWTC 3 cut(s) 218, 443, 727
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 4 cut(s) 81, 89, 394, 523
PleI GAGTC 1 cut(s) 919
PpsI GAGTC 1 cut(s) 919
PsiI TTATAA 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 867
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 3 cut(s) 163, 354, 624
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 841
PsuI RGATCY 2 cut(s) 209, 457
RseI CAYNNNNRTG 1 cut(s) 617
SaqAI TTAA 2 cut(s) 327, 348
SatI GCNGC 4 cut(s) 80, 88, 393, 522
Sau3AI GATC 4 cut(s) 209, 283, 338, 457
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 919
ScrFI CCNGG 2 cut(s) 573, 869
SduI GDGCHC 1 cut(s) 889
SexAI ACCWGGT 1 cut(s) 867
SfaNI GCATC 2 cut(s) 50, 266
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 617
Sse9I AATT 6 cut(s) 179, 300, 435, 549, 656, 792
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 3 cut(s) 23, 87, 914
SspMI CTAG 4 cut(s) 155, 716, 738, 917
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 571, 867
StyI CCWWGG 2 cut(s) 165, 876
TaaI ACNGT 4 cut(s) 75, 97, 776, 940
TasI AATT 6 cut(s) 179, 300, 435, 549, 656, 792
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 3 cut(s) 218, 443, 727
Tru1I TTAA 2 cut(s) 327, 348
Tru9I TTAA 2 cut(s) 327, 348
TscAI CASTG 2 cut(s) 100, 779
TseFI GTSAC 3 cut(s) 31, 113, 922
TseI GCWGC 3 cut(s) 79, 392, 521
Tsp45I GTSAC 3 cut(s) 31, 113, 922
TspDTI ATGAA 3 cut(s) 48, 239, 456
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 100, 779
XapI RAATTY 2 cut(s) 656, 792
XmiI GTMKAC 1 cut(s) 908
XmnI GAANNNNTTC 2 cut(s) 174, 657
XspI CTAG 4 cut(s) 155, 716, 738, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.