RLG00000030521

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009332
Physical Location & Seq
Forward (+)
62868520 .. 62871366
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030521

Sequence Viewer

Length: 531 bp
ATGATGACACGACTCTCCAGAACATTCCCTTCCGTAAAGCTCCCGAACCTTCACCCCTCCTCAATTCTATTACCATGTTCAACATCACTACCCAATATCAAATCAGAAGCAAAATCGAATTCTCCCTCAAAATCAAAACCAAAATCGAGGTCTTGGTCTGTGTACCTCATCCTTTCCACCACTGCACCCATCAAGACCTACGTTGGTGTCACCACCGATTTCTCTCGCCGTCTGAAACAACACAATGGTGAACTAAAAGGTGGGGCAAAGGCATCCCATGCTGGAAGACCATGGATTTGTGCGTGCCTAATACATGGTTTCAAGGACCAAAATGAAGCATGTGCATTTGAATCAAAATGGAAAACTTTATCAAGGAGTTTGCCTCGCAAAAGAAAGGACGATAACAAGGTAGAGCAAGTAAATGATTTGTCGCGCCCTCTACTACAACATAGGCAAGCAGCTCTAAATAGAGTTAAAGGTAAATTTGATTGTACTCGCTTAGAAATTGACTGGAAACTGAATCCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

177

Amino Acids

19.87

Weight (kDa)

10.22

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 52 - 126 3.6e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 529
AccII CGCG 1 cut(s) 433
AcsI RAATTY 2 cut(s) 118, 482
AfaI GTAC 2 cut(s) 164, 493
AgsI TTSAA 3 cut(s) 81, 322, 350
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 2 cut(s) 40, 461
AluI AGCT 2 cut(s) 40, 461
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 2 cut(s) 118, 482
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 279, 311
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 3 cut(s) 44, 202, 260
AvaII GGWCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 470
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 213
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmsI GCATC 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BsaJI CCNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 168, 272
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 470
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 290
BspFNI CGCG 1 cut(s) 433
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 304, 456
BstDEI CTNAG 1 cut(s) 499
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 168, 272
BstFNI CGCG 1 cut(s) 433
BstHHI GCGC 1 cut(s) 435
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 342
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 470
BstV2I GAAGAC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 2 cut(s) 168, 272
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 304, 456
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 163, 492
CviAII CATG 5 cut(s) 75, 278, 291, 314, 339
CviJI RGCY 2 cut(s) 40, 461
CviKI_1 RGCY 2 cut(s) 40, 461
CviQI GTAC 2 cut(s) 163, 492
DdeI CTNAG 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 5 cut(s) 78, 281, 294, 317, 342
FaiI YATR 7 cut(s) 76, 279, 292, 315, 340, 450, 529
FatI CATG 5 cut(s) 74, 277, 290, 313, 338
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 2 cut(s) 155, 259
Fsp4HI GCNGC 1 cut(s) 459
GlaI GCGC 1 cut(s) 434
GluI GCNGC 1 cut(s) 459
HhaI GCGC 1 cut(s) 435
Hin1II CATG 5 cut(s) 78, 281, 294, 317, 342
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 3 cut(s) 12, 350, 520
HphI GGTGA 3 cut(s) 44, 202, 260
Hpy166II GTNNAC 2 cut(s) 163, 251
Hpy188I TCNGA 2 cut(s) 106, 234
Hpy188III TCNNGA 3 cut(s) 18, 43, 193
Hpy8I GTNNAC 2 cut(s) 163, 251
HpyAV CCTTC 2 cut(s) 39, 59
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 185, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 5 cut(s) 78, 281, 294, 317, 342
HspAI GCGC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 45
LpnPI CCDG 3 cut(s) 31, 267, 496
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 1 cut(s) 281
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 1 cut(s) 297
MluCI AATT 4 cut(s) 63, 118, 482, 504
MlyI GAGTC 1 cut(s) 6
MnlI CCTC 7 cut(s) 67, 70, 136, 141, 176, 393, 447
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 246
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 1 cut(s) 278
NcoI CCATGG 1 cut(s) 290
NlaIII CATG 5 cut(s) 78, 281, 294, 317, 342
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 246
PfeI GAWTC 2 cut(s) 350, 520
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PsiI TTATAA 1 cut(s) 529
PspPI GGNCC 1 cut(s) 325
RsaI GTAC 2 cut(s) 164, 493
RsaNI GTAC 2 cut(s) 163, 492
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 1 cut(s) 6
SfaNI GCATC 1 cut(s) 281
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 246
Sse9I AATT 4 cut(s) 63, 118, 482, 504
StyI CCWWGG 1 cut(s) 290
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 116, 146
TasI AATT 4 cut(s) 63, 118, 482, 504
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 2 cut(s) 350, 520
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 2 cut(s) 118, 482
XceI RCATGY 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.