AT5G44710

Ribosomal protein S27 S33, mitochondrial (InterPro IPR013219)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007824
Physical Location & Seq
Forward (+)
18040910 .. 18042681
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44710.1

Sequence Viewer

Length: 309 bp
ATGGCTAGTGGTAGCCTGAAGAGCCTTATATCTTCAGCAGTGGGTCGAGGTGTGACCGAAGCAAGAGCAAGGATTTTCGGACATATGCTTAACCCAACGGGACAGAGATCTCCTCATAAGATATTAAGGAAGAAGCTTATTGGTGATAAGGTTGCAGAATGGTATCCATACGACATCAAGAATGAGGATCCCAATGTCTTGGCTAGAGAAGAAAAAGAGCGGATATCGAAGCTTGAGATGTTGAAACGTCGTGACAAAGGACCACCAAAGAAGGGTCATGGAAAACGTGCTGCGAAGCGTAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

102

Amino Acids

11.53

Weight (kDa)

10.6

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 18 - 91 7.2e-15 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 220
AciI CCGC 1 cut(s) 220
AclWI GGATC 2 cut(s) 182, 195
AcuI CTGAAG 2 cut(s) 18, 38
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 1 cut(s) 244
AluBI AGCT 2 cut(s) 136, 232
AluI AGCT 2 cut(s) 136, 232
AlwI GGATC 2 cut(s) 182, 195
ApeKI GCWGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 277
BciVI GTATCC 1 cut(s) 174
BfaI CTAG 2 cut(s) 6, 204
BfuI GTATCC 1 cut(s) 174
BglII AGATCT 1 cut(s) 107
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 97, 129
BpuEI CTTGAG 1 cut(s) 254
Bsc4I CCNNNNNNNGG 1 cut(s) 272
BseLI CCNNNNNNNGG 1 cut(s) 272
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 277
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 107, 187
BspACI CCGC 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 2 cut(s) 182, 195
BspQI GCTCTTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 220
BssMI GATC 2 cut(s) 107, 187
Bst6I CTCTTC 1 cut(s) 14
BstKTI GATC 2 cut(s) 110, 190
BstMBI GATC 2 cut(s) 107, 187
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 107, 187
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 107, 187
BsuI GTATCC 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 260
CviAII CATG 1 cut(s) 278
CviJI RGCY 6 cut(s) 5, 15, 24, 136, 203, 232
CviKI_1 RGCY 6 cut(s) 5, 15, 24, 136, 203, 232
DpnI GATC 2 cut(s) 109, 189
DpnII GATC 2 cut(s) 107, 187
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 2 cut(s) 18, 38
EcoRV GATATC 1 cut(s) 225
FaeI CATG 1 cut(s) 281
FaiI YATR 6 cut(s) 29, 84, 86, 117, 169, 279
FaqI GGGAC 1 cut(s) 114
FatI CATG 1 cut(s) 277
FauNDI CATATG 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 2 cut(s) 6, 204
GluI GCNGC 1 cut(s) 291
Hin1II CATG 1 cut(s) 281
HindIII AAGCTT 2 cut(s) 134, 230
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 2 cut(s) 178, 251
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 265
HpyCH4IV ACGT 2 cut(s) 247, 286
HpyCH4V TGCA 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpySE526I ACGT 2 cut(s) 247, 286
Hsp92II CATG 1 cut(s) 281
Kzo9I GATC 2 cut(s) 107, 187
LguI GCTCTTC 1 cut(s) 14
LpnPI CCDG 1 cut(s) 29
Lsp1109I GCAGC 1 cut(s) 277
MaeI CTAG 2 cut(s) 6, 204
MaeII ACGT 2 cut(s) 247, 286
MaeIII GTNAC 3 cut(s) 52, 251, 299
MalI GATC 2 cut(s) 109, 189
MbiI CCGCTC 1 cut(s) 220
MboI GATC 2 cut(s) 107, 187
MboII GAAGA 4 cut(s) 24, 31, 142, 221
MflI RGATCY 2 cut(s) 107, 187
MnlI CCTC 3 cut(s) 41, 123, 178
MseI TTAA 2 cut(s) 90, 125
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeI CATATG 1 cut(s) 84
NdeII GATC 2 cut(s) 107, 187
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 2 cut(s) 52, 251
PciSI GCTCTTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 260
PsuI RGATCY 2 cut(s) 107, 187
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 90, 125
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 2 cut(s) 107, 187
Sau96I GGNCC 1 cut(s) 260
SetI ASST 6 cut(s) 52, 138, 153, 234, 250, 289
SinI GGWCC 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SsiI CCGC 1 cut(s) 220
SspMI CTAG 2 cut(s) 6, 204
TaiI ACGT 2 cut(s) 250, 289
TaqI TCGA 2 cut(s) 46, 227
TaqII GACCGA 1 cut(s) 71
Tru1I TTAA 2 cut(s) 90, 125
Tru9I TTAA 2 cut(s) 90, 125
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 52, 251
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 52, 251
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 260
XspI CTAG 2 cut(s) 6, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.