Rh5DG155700

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007824
Physical Location & Seq
Reverse (-)
15835424 .. 15837086
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG155700.1

Sequence Viewer

Length: 321 bp
ATGGCGCCTGGCAGTTTAAAGAGCATTTTGACTGCGGCGGTTAATACTGGGGTGACTGAGGCTAGGGCAAGGATATTCGGGCATGTGCTTAACCCGACAGGCCTGAAGTCTGCGCATAAGATTTTGCGCAAGAAGCTGATTGGGGAGAAAGTTGCCCAGTGGTACCCACACGACATCAGGAAAGATGACCCCCTTATCATGGAAGCTGAGGAAAAAGAGTATGCTTTCTTCTCCATCTCCTTCACTCTATTGTCTAGCCATTTGCTTATGTTTCACTCTTGTATGAAATTTCGAACGAACAGTGTACTACTTCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

106

Amino Acids

12.01

Weight (kDa)

9.56

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 18 - 60 5.9e-10 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 114, 128
Acc65I GGTACC 1 cut(s) 162
AccB1I GGYRCC 2 cut(s) 4, 162
AciI CCGC 2 cut(s) 35, 38
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 164, 306
AfiI CCNNNNNNNGG 1 cut(s) 199
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 2 cut(s) 136, 206
AluI AGCT 2 cut(s) 136, 206
AoxI GGCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 287
Asp718I GGTACC 1 cut(s) 162
AspLEI GCGC 3 cut(s) 7, 115, 129
AsuHPI GGTGA 1 cut(s) 64
AsuII TTCGAA 1 cut(s) 292
BanI GGYRCC 2 cut(s) 4, 162
BarI GAAGNNNNNNTAC 2 cut(s) 212, 244
BbvCI CCTCAGC 1 cut(s) 207
BccI CCATC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 9
BfaI CTAG 2 cut(s) 63, 255
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 9
BmiI GGNNCC 2 cut(s) 6, 164
BmrFI CCNGG 1 cut(s) 9
BmrI ACTGGG 2 cut(s) 57, 151
BmuI ACTGGG 2 cut(s) 57, 151
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 52, 157
BseBI CCWGG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 48, 198
BseNI ACTGG 2 cut(s) 52, 157
BshFI GGCC 1 cut(s) 102
BshNI GGYRCC 2 cut(s) 4, 162
BslI CCNNNNNNNGG 1 cut(s) 199
BsnI GGCC 1 cut(s) 102
Bsp119I TTCGAA 1 cut(s) 292
BspACI CCGC 2 cut(s) 35, 38
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 49, 199
BspLI GGNNCC 2 cut(s) 6, 164
BspT104I TTCGAA 1 cut(s) 292
BspT107I GGYRCC 2 cut(s) 4, 162
BsrI ACTGG 2 cut(s) 52, 157
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 292
BstDEI CTNAG 2 cut(s) 57, 207
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 115, 129
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 7
BsuRI GGCC 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 164, 307
CfoI GCGC 3 cut(s) 7, 115, 129
Csp6I GTAC 2 cut(s) 163, 305
CviAII CATG 2 cut(s) 83, 199
CviJI RGCY 5 cut(s) 62, 102, 136, 206, 258
CviKI_1 RGCY 5 cut(s) 62, 102, 136, 206, 258
CviQI GTAC 2 cut(s) 163, 305
DdeI CTNAG 2 cut(s) 57, 207
DinI GGCGCC 1 cut(s) 6
DraI TTTAAA 1 cut(s) 18
Eco147I AGGCCT 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 7
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 2 cut(s) 86, 202
FaiI YATR 6 cut(s) 84, 117, 200, 222, 269, 284
FatI CATG 2 cut(s) 82, 198
Fnu4HI GCNGC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 2 cut(s) 63, 255
FspI TGCGCA 2 cut(s) 114, 128
GlaI GCGC 3 cut(s) 6, 114, 128
GluI GCNGC 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 102
HhaI GCGC 3 cut(s) 7, 115, 129
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 86, 202
Hin6I GCGC 3 cut(s) 5, 113, 127
HinP1I GCGC 3 cut(s) 5, 113, 127
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 305
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 2 cut(s) 57, 207
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 86, 202
HspAI GCGC 3 cut(s) 5, 113, 127
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 166
LpnPI CCDG 6 cut(s) 21, 33, 84, 116, 163, 170
MaeI CTAG 2 cut(s) 63, 255
MaeIII GTNAC 1 cut(s) 52
MboII GAAGA 1 cut(s) 220
MluCI AATT 1 cut(s) 287
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 2 cut(s) 52, 202
MseI TTAA 4 cut(s) 17, 42, 90, 319
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 133
NarI GGCGCC 1 cut(s) 5
NlaIII CATG 2 cut(s) 86, 202
NlaIV GGNNCC 2 cut(s) 6, 164
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 2 cut(s) 114, 128
NspI RCATGY 1 cut(s) 86
NspV TTCGAA 1 cut(s) 292
PceI AGGCCT 1 cut(s) 102
PkrI GCNGC 1 cut(s) 37
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 2 cut(s) 6, 164
RsaI GTAC 2 cut(s) 164, 306
RsaNI GTAC 2 cut(s) 163, 305
SaqAI TTAA 4 cut(s) 17, 42, 90, 319
SatI GCNGC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 9
SetI ASST 2 cut(s) 138, 208
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 292
Sse9I AATT 1 cut(s) 287
SseBI AGGCCT 1 cut(s) 102
SsiI CCGC 2 cut(s) 35, 38
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 63, 255
StuI AGGCCT 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 7
TaaI ACNGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 292
TasI AATT 1 cut(s) 287
TatI WGTACW 1 cut(s) 304
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 4 cut(s) 17, 42, 90, 319
Tru9I TTAA 4 cut(s) 17, 42, 90, 319
TscAI CASTG 2 cut(s) 164, 307
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 299, 302
TspRI CASTG 2 cut(s) 164, 307
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 86
XspI CTAG 2 cut(s) 63, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.