MD05G1245000.v1.1

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007824
Physical Location & Seq
Forward (+)
37717379 .. 37718993
1615 bp
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UTR
Exon/CDS
Intron
MD05G1245000.v1.1.491

Sequence Viewer

Length: 288 bp
ATGTTGGTTGCGGCAGTCAATACCGGGGTGACTGAGGCTAGGGCGAGGATATTCGGGCACGTAATTAACCCAACGGGCCAGAGATCTGCCCATAAGATTCTGCGCAAGAAGTTGATCGGTGAGAAAGTTGCCCAGTGGTACCCTTATGACATCAAGAAAGATGACCCCCGAATCATGGCTATGGAAGAACAAGAGCGCTTATCCAAGCTTGAAATGTTGAAGCGTCGAGGAAAAGGACCACCGAAGAAGGGTCAAGGAAGGCGTGCTGCCAAGCGTAACAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

96

Amino Acids

10.88

Weight (kDa)

10.9

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 10 - 83 2.6e-16 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AciI CCGC 1 cut(s) 11
AfaI GTAC 1 cut(s) 140
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 175, 248
AgsI TTSAA 2 cut(s) 212, 220
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 2 cut(s) 105, 198
AspS9I GGNCC 2 cut(s) 76, 236
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 40, 131
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 253
BcnI CCSGG 1 cut(s) 25
BfaI CTAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 199
BglII AGATCT 1 cut(s) 83
BisI GCNGC 2 cut(s) 12, 267
BlsI GCNGC 2 cut(s) 13, 268
Bme1390I CCNGG 1 cut(s) 25
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 76, 236
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 25
BmrI ACTGGG 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 127
BpuMI CCSGG 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 248
Bse1I ACTGG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 175, 248
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 133
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 1 cut(s) 253
BshFI GGCC 1 cut(s) 78
BshNI GGYRCC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 175, 248
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 2 cut(s) 83, 114
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 138
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 2 cut(s) 83, 114
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 105, 198
BstKTI GATC 2 cut(s) 86, 117
BstMBI GATC 2 cut(s) 83, 114
BstSCI CCNGG 1 cut(s) 23
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 2 cut(s) 105, 198
Cfr13I GGNCC 2 cut(s) 76, 236
CseI GACGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 139
CviAII CATG 1 cut(s) 175
CviJI RGCY 4 cut(s) 38, 78, 179, 208
CviKI_1 RGCY 4 cut(s) 38, 78, 179, 208
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 33
DpnI GATC 2 cut(s) 85, 116
DpnII GATC 2 cut(s) 83, 114
Eco47I GGWCC 1 cut(s) 236
Eco47III AGCGCT 1 cut(s) 197
FaeI CATG 1 cut(s) 178
FaiI YATR 4 cut(s) 93, 147, 176, 182
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 12, 267
Fsp4HI GCNGC 2 cut(s) 12, 267
FspBI CTAG 1 cut(s) 39
FspI TGCGCA 1 cut(s) 104
GlaI GCGC 2 cut(s) 104, 197
GluI GCNGC 2 cut(s) 12, 267
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 212
HhaI GCGC 2 cut(s) 105, 198
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 2 cut(s) 103, 196
HinP1I GCGC 2 cut(s) 103, 196
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 2 cut(s) 97, 171
HpaII CCGG 1 cut(s) 24
HphI GGTGA 2 cut(s) 40, 131
Hpy188III TCNNGA 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 2 cut(s) 241, 252
HpyCH4IV ACGT 1 cut(s) 60
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 2 cut(s) 103, 196
KpnI GGTACC 1 cut(s) 142
Kzo9I GATC 2 cut(s) 83, 114
LpnPI CCDG 3 cut(s) 37, 92, 146
Lsp1109I GCAGC 1 cut(s) 253
MaeI CTAG 1 cut(s) 39
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 2 cut(s) 28, 275
MalI GATC 2 cut(s) 85, 116
MboI GATC 2 cut(s) 83, 114
MboII GAAGA 2 cut(s) 197, 256
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 1 cut(s) 63
MnlI CCTC 3 cut(s) 28, 39, 221
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 25
NciI CCSGG 1 cut(s) 25
NdeII GATC 2 cut(s) 83, 114
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 104
PfeI GAWTC 2 cut(s) 97, 171
PkrI GCNGC 2 cut(s) 13, 268
Ppu21I YACGTR 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 76, 236
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 12, 267
Sau3AI GATC 2 cut(s) 83, 114
Sau96I GGNCC 2 cut(s) 76, 236
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 1 cut(s) 60
SetI ASST 2 cut(s) 63, 210
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 1 cut(s) 63
SsiI CCGC 1 cut(s) 11
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 23
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 226
TasI AATT 1 cut(s) 63
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 97, 171
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 28
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 236
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.