FvH4_3g13100

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007824
Physical Location & Seq
Reverse (-)
7826422 .. 7827164
743 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13100.t1

Sequence Viewer

Length: 306 bp
ATGGCTTCTCGAAGTCTGAAGAGCATTTTGACTGCCGCGGTTAATACTGGGGTGACTGAGGCCAGGGCAAGGATATTCGGGCATGTGCTTAACCCGACAGGGCTGAAGTCTGCGCATAAGATCTTAAGGAAGAAGCTGATTGGGGAGAAAGTTGCCCAGTGGTACCCGCATGACATCAGGAAAGACGACCCCCTTGTCATGGAAGCCGAGGAAAAAGAGCGCTTAAACAAGCTTGAAATGTTGAAGCGTCGAGGAAAAGGGCCACCCAAGAAGGGTCAAGGAAAGCGTTCCAAGCGCAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

102

Amino Acids

11.41

Weight (kDa)

10.76

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 18 - 91 2.2e-15 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc16I TGCGCA 1 cut(s) 114
Acc65I GGTACC 1 cut(s) 162
AccB1I GGYRCC 1 cut(s) 162
AccII CGCG 1 cut(s) 38
AciI CCGC 3 cut(s) 36, 38, 167
AcuI CTGAAG 2 cut(s) 38, 125
AfaI GTAC 1 cut(s) 164
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 69, 199, 272
AflII CTTAAG 1 cut(s) 124
AgsI TTSAA 2 cut(s) 236, 244
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 3 cut(s) 136, 232, 303
AluI AGCT 3 cut(s) 136, 232, 303
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 2 cut(s) 60, 260
Asp700I GAANNNNTTC 1 cut(s) 286
Asp718I GGTACC 1 cut(s) 162
AspLEI GCGC 3 cut(s) 115, 222, 297
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 64
BanI GGYRCC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 64
BfaI CTAG 1 cut(s) 304
BfoI RGCGCY 1 cut(s) 223
BfrI CTTAAG 1 cut(s) 124
BglII AGATCT 1 cut(s) 120
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 64
BmrI ACTGGG 2 cut(s) 57, 151
BmuI ACTGGG 2 cut(s) 57, 151
BsaJI CCNNGG 3 cut(s) 36, 63, 207
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 199, 272
Bse1I ACTGG 2 cut(s) 52, 157
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 36, 63, 207
BseLI CCNNNNNNNGG 3 cut(s) 69, 199, 272
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 2 cut(s) 52, 157
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 62, 262
BshNI GGYRCC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 69, 199, 272
BsnI GGCC 2 cut(s) 62, 262
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 3 cut(s) 36, 38, 167
BspANI GGCC 2 cut(s) 62, 262
BspCNI CTCAG 1 cut(s) 49
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 164
BspQI GCTCTTC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 124
BsrI ACTGG 2 cut(s) 52, 157
BssECI CCNNGG 3 cut(s) 36, 63, 207
BssMI GATC 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 14
BstAFI CTTAAG 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 36
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 3 cut(s) 115, 222, 297
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 62
BstUI CGCG 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 2 cut(s) 62, 262
BtgI CCRYGG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 164
CfoI GCGC 3 cut(s) 115, 222, 297
Cfr13I GGNCC 1 cut(s) 260
Cfr42I CCGCGG 1 cut(s) 39
CseI GACGC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 163
CviAII CATG 3 cut(s) 83, 170, 199
CviJI RGCY 8 cut(s) 5, 62, 103, 136, 206, 232, 262, 303
CviKI_1 RGCY 8 cut(s) 5, 62, 103, 136, 206, 232, 262, 303
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 57
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco47III AGCGCT 1 cut(s) 221
Eco57I CTGAAG 2 cut(s) 38, 125
EcoRII CCWGG 1 cut(s) 62
FaeI CATG 3 cut(s) 86, 173, 202
FaiI YATR 4 cut(s) 84, 117, 171, 200
FatI CATG 3 cut(s) 82, 169, 198
FauI CCCGC 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 1 cut(s) 304
FspI TGCGCA 1 cut(s) 114
GlaI GCGC 3 cut(s) 114, 221, 296
GluI GCNGC 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 2 cut(s) 62, 262
HgaI GACGC 1 cut(s) 236
HhaI GCGC 3 cut(s) 115, 222, 297
Hin1II CATG 3 cut(s) 86, 173, 202
Hin6I GCGC 3 cut(s) 113, 220, 295
HinP1I GCGC 3 cut(s) 113, 220, 295
HindIII AAGCTT 1 cut(s) 230
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 9, 178
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 1 cut(s) 57
Hsp92II CATG 3 cut(s) 86, 173, 202
HspAI GCGC 3 cut(s) 113, 220, 295
KpnI GGTACC 1 cut(s) 166
KspI CCGCGG 1 cut(s) 39
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 14
LpnPI CCDG 6 cut(s) 33, 49, 76, 84, 163, 170
MaeI CTAG 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 52
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 2 cut(s) 31, 142
MflI RGATCY 1 cut(s) 120
MnlI CCTC 3 cut(s) 52, 202, 245
MroXI GAANNNNTTC 1 cut(s) 286
MseI TTAA 4 cut(s) 42, 90, 125, 224
MspA1I CMGCKG 1 cut(s) 38
MspCI CTTAAG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 1 cut(s) 120
NlaIII CATG 3 cut(s) 86, 173, 202
NlaIV GGNNCC 1 cut(s) 164
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 1 cut(s) 114
NspI RCATGY 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 286
PkrI GCNGC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 260
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SacII CCGCGG 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 4 cut(s) 42, 90, 125, 224
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 64
SetI ASST 3 cut(s) 138, 234, 305
Sfr303I CCGCGG 1 cut(s) 39
SgrBI CCGCGG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
SsiI CCGC 3 cut(s) 36, 38, 167
SspMI CTAG 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 62
TaqI TCGA 2 cut(s) 10, 250
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 4 cut(s) 42, 90, 125, 224
Tru9I TTAA 4 cut(s) 42, 90, 125, 224
TscAI CASTG 1 cut(s) 164
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspRI CASTG 1 cut(s) 164
Vha464I CTTAAG 1 cut(s) 124
XceI RCATGY 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 286
XspI CTAG 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.