MD10G1226300.v1.1

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007824
Physical Location & Seq
Forward (+)
32349881 .. 32351547
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1226300.v1.1.491

Sequence Viewer

Length: 288 bp
ATGTTGGTTGCGGCAGTCAGTACTGGGGTGACTGAGGCTAGGGCGAGGATATTCGGGCACATACTTAACCCAACCGGCCAGAGATCTGCCCATAAGATTTTGCGCAAGAAGTTGATTGGTGAGAAAGTTGCCCAGTGGTACCCATATGACATCAAGAAAGATGACCGCCGAATCATGGCTATGGAAGAACAAGAGCGCTTATCCAAGCTCGAAATGTTGAAACGTCGAGGAAAAGGACCACCGAAGAAGGGTCAAGGAAGGCGTGCTGCCAAGCGTAACAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

96

Amino Acids

10.92

Weight (kDa)

11.07

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 10 - 83 3.8e-16 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AciI CCGC 2 cut(s) 11, 166
AcoI YGGCCR 1 cut(s) 76
AfaI GTAC 2 cut(s) 22, 140
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 175, 248
AgsI TTSAA 1 cut(s) 220
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 2 cut(s) 105, 198
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 40, 131
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 253
BfaI CTAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 199
BglII AGATCT 1 cut(s) 83
BisI GCNGC 2 cut(s) 12, 267
BlsI GCNGC 2 cut(s) 13, 268
BmcAI AGTACT 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 140
BmrI ACTGGG 2 cut(s) 33, 127
BmuI ACTGGG 2 cut(s) 33, 127
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 248
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 28, 133
BseLI CCNNNNNNNGG 2 cut(s) 175, 248
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 28, 133
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 1 cut(s) 253
BshFI GGCC 1 cut(s) 78
BshNI GGYRCC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 175, 248
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 83
BspACI CCGC 2 cut(s) 11, 166
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 2 cut(s) 28, 133
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 105, 198
BstKTI GATC 1 cut(s) 86
BstMBI GATC 1 cut(s) 83
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 2 cut(s) 105, 198
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 21, 139
CviAII CATG 1 cut(s) 175
CviJI RGCY 4 cut(s) 38, 78, 179, 208
CviKI_1 RGCY 4 cut(s) 38, 78, 179, 208
CviQI GTAC 2 cut(s) 21, 139
DdeI CTNAG 1 cut(s) 33
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
EaeI YGGCCR 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 236
Eco47III AGCGCT 1 cut(s) 197
FaeI CATG 1 cut(s) 178
FaiI YATR 6 cut(s) 62, 93, 145, 147, 176, 182
FatI CATG 1 cut(s) 174
FauNDI CATATG 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 12, 267
Fsp4HI GCNGC 2 cut(s) 12, 267
FspBI CTAG 1 cut(s) 39
FspI TGCGCA 1 cut(s) 104
GlaI GCGC 2 cut(s) 104, 197
GluI GCNGC 2 cut(s) 12, 267
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 75
HhaI GCGC 2 cut(s) 105, 198
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 2 cut(s) 103, 196
HinP1I GCGC 2 cut(s) 103, 196
HinfI GANTC 1 cut(s) 171
HpaII CCGG 1 cut(s) 75
HphI GGTGA 2 cut(s) 40, 131
Hpy188III TCNNGA 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 2 cut(s) 241, 252
HpyCH4IV ACGT 1 cut(s) 223
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 223
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 2 cut(s) 103, 196
KpnI GGTACC 1 cut(s) 142
Kzo9I GATC 1 cut(s) 83
LpnPI CCDG 4 cut(s) 9, 88, 92, 146
Lsp1109I GCAGC 1 cut(s) 253
MaeI CTAG 1 cut(s) 39
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 2 cut(s) 28, 275
MalI GATC 1 cut(s) 85
MboI GATC 1 cut(s) 83
MboII GAAGA 2 cut(s) 197, 256
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 60
MnlI CCTC 3 cut(s) 28, 39, 221
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 1 cut(s) 75
NdeI CATATG 1 cut(s) 145
NdeII GATC 1 cut(s) 83
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 104
PfeI GAWTC 1 cut(s) 171
PkrI GCNGC 2 cut(s) 13, 268
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 1 cut(s) 236
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 2 cut(s) 22, 140
RsaNI GTAC 2 cut(s) 21, 139
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 12, 267
Sau3AI GATC 1 cut(s) 83
Sau96I GGNCC 1 cut(s) 236
ScaI AGTACT 1 cut(s) 22
SduI GDGCHC 1 cut(s) 60
SetI ASST 2 cut(s) 210, 226
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 179
SsiI CCGC 2 cut(s) 11, 166
SspMI CTAG 1 cut(s) 39
TaiI ACGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 210, 226
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 28
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 236
XspI CTAG 1 cut(s) 39
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.