Rw5G013870

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007824
Physical Location & Seq
Reverse (-)
16374142 .. 16375354
1213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013870.1

Sequence Viewer

Length: 339 bp
ATGTTTTTCCATTTTTCAGAGAGGCAAAGTGAGATGGCGCCTGGCAGTTTAAAGAGCATTTTGACTGCGGCGGTTAATACTGGGGTGACTGAGGCTAGGGCAAGGATATTCGGGCATGTGCTTAACCCGACAGGCCTGAAGTCTGCGCATAAGATTTTGCGCAAGAAGCTGATTGGGGAGAAAGTTGCCCAGTGGTACCCACACGACATCAGGAAAGATGACCCCCTTATCATGGAAGCTGAGGAAAAAGAGCGCACAAACAAGCTTGAAATGTTGAAGCGTCGAGGAAAAGGACTACCCAAGAAGGGTCAAGGAAAACGTTCCAAGCGCAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

112

Amino Acids

12.77

Weight (kDa)

10.47

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 29 - 102 2.5e-14 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 147, 161
Acc65I GGTACC 1 cut(s) 195
AccB1I GGYRCC 2 cut(s) 37, 195
AciI CCGC 2 cut(s) 68, 71
AclI AACGTT 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 232, 305
AgsI TTSAA 2 cut(s) 269, 277
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 4 cut(s) 169, 239, 265, 336
AluI AGCT 4 cut(s) 169, 239, 265, 336
AoxI GGCC 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 319
Asp718I GGTACC 1 cut(s) 195
AspLEI GCGC 5 cut(s) 40, 148, 162, 255, 330
AsuHPI GGTGA 1 cut(s) 97
BanI GGYRCC 2 cut(s) 37, 195
BbvCI CCTCAGC 1 cut(s) 240
BccI CCATC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 42
BfaI CTAG 2 cut(s) 96, 337
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 42
BmiI GGNNCC 2 cut(s) 39, 197
BmrFI CCNGG 1 cut(s) 42
BmrI ACTGGG 2 cut(s) 90, 184
BmuI ACTGGG 2 cut(s) 90, 184
Bpu10I CCTNAGC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 38
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 305
Bse1I ACTGG 2 cut(s) 85, 190
BseBI CCWGG 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 232, 305
BseMII CTCAG 2 cut(s) 81, 231
BseNI ACTGG 2 cut(s) 85, 190
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 2 cut(s) 37, 195
BslI CCNNNNNNNGG 2 cut(s) 232, 305
BsnI GGCC 1 cut(s) 135
BspACI CCGC 2 cut(s) 68, 71
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 82, 232
BspLI GGNNCC 2 cut(s) 39, 197
BspT107I GGYRCC 2 cut(s) 37, 195
BsrI ACTGG 2 cut(s) 85, 190
BssNI GRCGYC 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 90, 240
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 5 cut(s) 40, 148, 162, 255, 330
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 40
BsuRI GGCC 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 197
CfoI GCGC 5 cut(s) 40, 148, 162, 255, 330
CseI GACGC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 196
CviAII CATG 2 cut(s) 116, 232
CviJI RGCY 6 cut(s) 95, 135, 169, 239, 265, 336
CviKI_1 RGCY 6 cut(s) 95, 135, 169, 239, 265, 336
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 90, 240
DinI GGCGCC 1 cut(s) 39
DraI TTTAAA 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
FaeI CATG 2 cut(s) 119, 235
FaiI YATR 3 cut(s) 117, 150, 233
FatI CATG 2 cut(s) 115, 231
Fnu4HI GCNGC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 2 cut(s) 96, 337
FspI TGCGCA 2 cut(s) 147, 161
GlaI GCGC 5 cut(s) 39, 147, 161, 254, 329
GluI GCNGC 1 cut(s) 69
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 1 cut(s) 135
HgaI GACGC 1 cut(s) 269
HhaI GCGC 5 cut(s) 40, 148, 162, 255, 330
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 2 cut(s) 119, 235
Hin6I GCGC 5 cut(s) 38, 146, 160, 253, 328
HinP1I GCGC 5 cut(s) 38, 146, 160, 253, 328
HindIII AAGCTT 1 cut(s) 263
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 90, 240
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 2 cut(s) 119, 235
HspAI GCGC 5 cut(s) 38, 146, 160, 253, 328
KasI GGCGCC 1 cut(s) 37
KpnI GGTACC 1 cut(s) 199
LpnPI CCDG 7 cut(s) 27, 54, 66, 117, 149, 196, 203
MaeI CTAG 2 cut(s) 96, 337
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 1 cut(s) 85
Mly113I GGCGCC 1 cut(s) 38
MnlI CCTC 4 cut(s) 15, 85, 235, 278
MroXI GAANNNNTTC 1 cut(s) 319
MseI TTAA 3 cut(s) 50, 75, 123
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 166
NarI GGCGCC 1 cut(s) 38
NlaIII CATG 2 cut(s) 119, 235
NlaIV GGNNCC 2 cut(s) 39, 197
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 2 cut(s) 147, 161
NspI RCATGY 1 cut(s) 119
PceI AGGCCT 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 70
PluTI GGCGCC 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 39, 197
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 3 cut(s) 50, 75, 123
SatI GCNGC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 42
SetI ASST 5 cut(s) 171, 241, 267, 322, 338
SfoI GGCGCC 1 cut(s) 39
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 2 cut(s) 68, 71
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 96, 337
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 40
TaiI ACGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 283
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 3 cut(s) 50, 75, 123
Tru9I TTAA 3 cut(s) 50, 75, 123
TscAI CASTG 1 cut(s) 197
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspRI CASTG 1 cut(s) 197
XceI RCATGY 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 2 cut(s) 96, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.