RLG00000022798

Mitochondrial ribosomal subunit S27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007824
Physical Location & Seq
Forward (+)
12368616 .. 12371789
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022798

Sequence Viewer

Length: 711 bp
ATGGTTGTGAAATTGGGTTGCGTTATCAACAAGGAGCTAGAGGGAGGGAAGACGACGACGTTGAGGAAAGCGGGAGAGCTTTCTTTATGGATTGGGGATTTTGAAGGTTCCGGTGGAAGCTCAGGTCAGGTTAGAATTTCATATGCTATCATTACTTGTGAGAAGGAGAAGAGGTCGAATCAGTTTGTGTTTCTTTTGAAAAAGTACATTGTCTGTACAATCCGCACTGATATATCAAGCCTCCTTTTCTCTATGTTGGTTTTGGAAGTGAGAGTCGCTCTTGAAGAGACTAAACCTGAAACCAAAGGCTTTGCAGATCCTATTTTGAAGTTTTGCAGTTTTCTAAGGTTGAATTGTTCCCAAAAGGCTTGCAAATTTGGTCTCTTTGACAGAAGGCAAAGTGGGATGGCGTCTGGCAGTCTAAAGAGCATTTTGACTGCGGCGGTTAATACTGGGGTGACTGAGGCTAGGGCAAGGATATTCGGGCATGTGCTTAACCAGACAGGCTTGAAGTCTGCGCATAAGATTTTGCGCAAGAAGCTGATTGGGGAGAAAGTTGCCCAGTGGTACCCACATGACATTAGGAAAGATGACCCCCTTATTATGGAAGCTGAGGAAAAAGAGCGCACAAACAAGCTTGAAATGTTGAAGCGTCGAGGAAAAGGACCACCCAAGAAGGGTCAAGGAAAGCGTTCCAAGCGCAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S33

Protein Analysis

237

Amino Acids

26.39

Weight (kDa)

9.97

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S33 PF08293 153 - 226 2.1e-13 Mitochondrial ribosomal subunit S27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 519, 533
Acc65I GGTACC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 567
AciI CCGC 4 cut(s) 71, 223, 440, 443
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 2 cut(s) 135, 374
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 3 cut(s) 206, 217, 569
AfiI CCNNNNNNNGG 2 cut(s) 604, 677
AgsI TTSAA 8 cut(s) 104, 199, 284, 328, 352, 511, 641, 649
AluBI AGCT 7 cut(s) 37, 79, 120, 541, 611, 637, 708
AluI AGCT 7 cut(s) 37, 79, 120, 541, 611, 637, 708
Alw26I GTCTC 2 cut(s) 281, 386
AlwI GGATC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 135, 374
Asp700I GAANNNNTTC 1 cut(s) 691
Asp718I GGTACC 1 cut(s) 567
AspLEI GCGC 4 cut(s) 520, 534, 627, 702
AspS9I GGNCC 1 cut(s) 665
AsuHPI GGTGA 1 cut(s) 469
AvaII GGWCC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 56
BbvCI CCTCAGC 1 cut(s) 612
BccI CCATC 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 281, 386
BfaI CTAG 3 cut(s) 38, 468, 709
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme18I GGWCC 1 cut(s) 665
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 2 cut(s) 109, 569
BmrI ACTGGG 2 cut(s) 462, 556
BmuI ACTGGG 2 cut(s) 462, 556
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 262, 294
Bpu10I CCTNAGC 2 cut(s) 121, 612
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 386
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 677
Bse1I ACTGG 2 cut(s) 457, 562
BseGI GGATG 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 604, 677
BseMII CTCAG 3 cut(s) 135, 453, 603
BseNI ACTGG 2 cut(s) 457, 562
BshNI GGYRCC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 604, 677
BsmAI GTCTC 2 cut(s) 281, 386
Bso31I GGTCTC 1 cut(s) 386
Bsp1407I TGTACA 1 cut(s) 215
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 4 cut(s) 71, 223, 440, 443
BspCNI CTCAG 3 cut(s) 134, 454, 604
BspLI GGNNCC 2 cut(s) 109, 569
BspPI GGATC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 567
BspTNI GGTCTC 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 2 cut(s) 457, 562
BssMI GATC 1 cut(s) 316
BssNI GRCGYC 1 cut(s) 410
Bst6I CTCTTC 2 cut(s) 164, 279
BstACI GRCGYC 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 4 cut(s) 121, 344, 462, 612
BstF5I GGATG 1 cut(s) 411
BstHHI GCGC 4 cut(s) 520, 534, 627, 702
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 2 cut(s) 281, 386
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 2 cut(s) 538, 697
BstNSI RCATGY 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BtsCI GGATG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 225, 569
Cac8I GCNNGC 1 cut(s) 370
CfoI GCGC 4 cut(s) 520, 534, 627, 702
Cfr13I GGNCC 1 cut(s) 665
CseI GACGC 2 cut(s) 399, 641
Csp6I GTAC 3 cut(s) 205, 216, 568
CviAII CATG 2 cut(s) 488, 575
CviQI GTAC 3 cut(s) 205, 216, 568
DdeI CTNAG 4 cut(s) 121, 344, 462, 612
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 164, 279
EarI CTCTTC 2 cut(s) 164, 279
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 665
FaeI CATG 2 cut(s) 491, 578
FaiI YATR 9 cut(s) 88, 142, 144, 233, 254, 489, 522, 576, 605
FatI CATG 2 cut(s) 487, 574
FauI CCCGC 1 cut(s) 64
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 1 cut(s) 441
FspBI CTAG 3 cut(s) 38, 468, 709
FspI TGCGCA 2 cut(s) 519, 533
GlaI GCGC 4 cut(s) 519, 533, 626, 701
GluI GCNGC 1 cut(s) 441
HapII CCGG 1 cut(s) 111
HgaI GACGC 2 cut(s) 399, 641
HhaI GCGC 4 cut(s) 520, 534, 627, 702
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 2 cut(s) 491, 578
Hin6I GCGC 4 cut(s) 518, 532, 625, 700
HinP1I GCGC 4 cut(s) 518, 532, 625, 700
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 2 cut(s) 178, 273
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 469
Hpy188III TCNNGA 1 cut(s) 281
Hpy99I CGWCG 3 cut(s) 58, 61, 657
HpyAV CCTTC 4 cut(s) 98, 157, 387, 670
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 3 cut(s) 314, 336, 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 538, 697
HpyF3I CTNAG 4 cut(s) 121, 344, 462, 612
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 2 cut(s) 491, 578
HspAI GCGC 4 cut(s) 518, 532, 625, 700
KpnI GGTACC 1 cut(s) 571
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 9 cut(s) 108, 113, 124, 309, 399, 438, 489, 512, 575
MaeI CTAG 3 cut(s) 38, 468, 709
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 457
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 3 cut(s) 61, 181, 296
MflI RGATCY 1 cut(s) 316
MluCI AATT 4 cut(s) 11, 135, 352, 374
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 8 cut(s) 34, 38, 57, 165, 251, 457, 607, 650
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 2 cut(s) 447, 495
MspI CCGG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 538, 697
NdeI CATATG 1 cut(s) 142
NdeII GATC 1 cut(s) 316
NlaIII CATG 2 cut(s) 491, 578
NlaIV GGNNCC 2 cut(s) 109, 569
NmuCI GTSAC 1 cut(s) 457
NsbI TGCGCA 2 cut(s) 519, 533
NspI RCATGY 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 1 cut(s) 442
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PspN4I GGNNCC 2 cut(s) 109, 569
PspPI GGNCC 1 cut(s) 665
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 3 cut(s) 206, 217, 569
RsaNI GTAC 3 cut(s) 205, 216, 568
SaqAI TTAA 2 cut(s) 447, 495
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 1 cut(s) 282
SinI GGWCC 1 cut(s) 665
Sse9I AATT 4 cut(s) 11, 135, 352, 374
SsiI CCGC 4 cut(s) 71, 223, 440, 443
SspMI CTAG 3 cut(s) 38, 468, 709
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 176, 655
TasI AATT 4 cut(s) 11, 135, 352, 374
TatI WGTACW 2 cut(s) 204, 215
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 447, 495
Tru9I TTAA 2 cut(s) 447, 495
TscAI CASTG 2 cut(s) 232, 569
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 1 cut(s) 129
TspRI CASTG 2 cut(s) 232, 569
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 2 cut(s) 135, 374
XceI RCATGY 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 3 cut(s) 38, 468, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.