Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Reverse (-)

18075562 .. 18080160

Gene Structure 4599 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G44790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGCGCCGAGTAGACGGGATTTACAGCTTACTCCGGTCACCGGAGGTTCTTCTTCCCAGATCAGTGATATGGAAGAAGTTGGTCTTCTCGATTCGTATCACAACGAAGCGAACGCCGATGATATTTTGACTAAAATCGAAGAAGGAAGAGATGTTTCCGGTTTAAGGAAGATTCAGGTCGGAGTCACCGGTATGACTTGTGCTGCTTGTTCTAATTCCGTTGAAGCAGCTTTGATGAACGTTAATGGCGTCTTCAAAGCCTCTGTTGCTTTGTTACAGAATCGAGCCGATGTCGTCTTCGACCCTAATTTAGTCAAGGAAGAGGATATTAAAGAAGCTATAGAGGATGCTGGATTTGAGGCTGAGATATTGGCTGAAGAACAGACACAAGCTACTTTGGTGGGTCAATTTACAATTGGAGGTATGACTTGTGCAGCTTGTGTTAACTCTGTTGAAGGTATTCTAAGAGATCTTCCTGGAGTGAAAAGAGCAGTGGTAGCTTTGTCTACATCATTAGGAGAAGTTGAGTATGATCCAAATGTTATTAACAAGGATGATATTGTTAATGCGATTGAAGATGCTGGGTTTGAAGGTTCGCTTGTGCAGAGTAATCAGCAGGATAAGCTTGTTTTAAGGGTGGATGGGATTTTGAATGAGCTTGATGCTCAGGTTTTAGAAGGGATACTTACTAGATTGAATGGGGTTAGACAGTTTCGTCTTGACAGGATATCTGGAGAGCTTGAGGTTGTGTTTGATCCGGAAGTTGTTAGTTCGAGATCTTTGGTTGATGGGATTGAAGAAGATGGATTTGGGAAGTTTAAGTTGCGTGTTATGAGCCCTTATGAAAGGTTGTCTTCGAAAGATACTGGAGAGGCCTCAAATATGTTTCGGCGTTTTATCTCCAGTCTTGTTCTTAGTATTCCTCTCTTCTTCATCCAAGTAATCTGCCCCCACATTGCTCTTTTTGATGCCTTACTGGTATGGAGATGTGGACCCTTCATGATGGGTGATTGGTTGAAATGGGCCTTGGTAAGTGTTATTCAGTTTGTTATCGGCAAGCGATTCTATGTTGCAGCATGGAGAGCTCTACGAAATGGTTCAACTAACATGGATGTGCTCGTCGCTCTGGGCACGTCTGCTTCTTACTTCTACTCTGTTGGGGCTCTTTTATATGGAGCAGTCACTGGATTTTGGTCTCCAACATACTTTGATGCAAGCGCCATGCTGATAACTTTTGTCTTACTGGGAAAATATTTAGAGTCTCTTGCAAAGGGGAAAACCTCGGATGCTATGAAGAAACTAGTACAACTTACGCCAGCAACAGCGATTTTACTAACTGAAGGCAAAGGAGGAAAGCTTGTAGGGGAAAGGGAGATAGATGCATTATTGATTCAGCCTGGTGATACATTAAAAGTTCATCCCGGTGCAAAGATCCCTGCAGATGGTGTTGTGGTGTGGGGTTCAAGTTACGTTAATGAAAGTATGGTGACTGGTGAATCAGTTCCTGTATCAAAAGAGGTTGATTCACCAGTAATCGGGGGTACTATTAATATGCATGGTGCTCTTCACATGAAAGCTACAAAGGTGGGATCTGATGCAGTTCTGAGCCAGATTATTAGTTTGGTCGAGACCGCCCAAATGTCAAAAGCTCCTATTCAGAAATTTGCGGATTATGTTGCAAGTATTTTTGTTCCGGTAGTGATTACTTTGGCATTGTTCACCTTAGTAGGCTGGTCAATTGGTGGAGCTGTTGGAGCTTACCCAGACGAATGGCTTCCGGAGAACGGGACTCACTTTGTTTTCTCGCTTATGTTCTCAATATCCGTTGTGGTAATTGCTTGTCCTTGTGCACTTGGCTTGGCAACACCGACTGCTGTTATGGTGGCAACAGGAGTTGGAGCAACCAATGGTGTATTGATCAAAGGAGGTGATGCATTAGAGAAAGCTCACAAAGTGAAGTATGTAATCTTTGACAAAACAGGCACCTTAACTCAAGGAAAAGCTACTGTGACAACCACAAAGGTCTTCTCAGAGATGGACCGTGGAGAATTCCTTACACTTGTTGCGTCTGCTGAGGCTAGCAGTGAACACCCATTGGCAAAAGCTATAGTTGCGTACGCTCGGCATTTCCACTTCTTTGATGAATCCACTGAAGATGGCGAAACAAATAACAAAGATTTGCAAAACTCTGGATGGTTACTTGATACCTCGGATTTCTCTGCTCTTCCCGGAAAAGGGATTCAGTGCTTAGTCAATGAGAAGATGATTCTGGTGGGAAACCGTAAACTTATGTCAGAAAATGCCATAAACATCCCAGACCATGTCGAGAAATTTGTGGAAGATCTTGAAGAAAGCGGAAAGACGGGAGTCATTGTAGCCTATAATGGGAAATTAGTTGGTGTGATGGGAATCGCGGATCCTCTAAAGCGAGAAGCCGCTTTGGTTGTGGAAGGTCTCCTTAGAATGGGTGTTCGACCCATAATGGTTACAGGTGATAACTGGAGAACAGCAAGAGCAGTCGCCAAAGAGGTTGGTATCGAAGATGTGAGAGCAGAAGTAATGCCAGCTGGTAAAGCTGACGTTATACGTTCGCTGCAAAAGGACGGAAGCACAGTAGCCATGGTAGGAGACGGAATCAATGACTCTCCCGCTCTAGCCGCTGCAGATGTGGGAATGGCAATTGGTGCAGGAACAGATGTAGCAATAGAAGCAGCAGATTACGTTCTGATGAGAAACAACTTAGAAGACGTTATAACAGCCATCGATCTCTCTCGAAAAACCTTAACACGAATCCGATTAAATTACGTCTTCGCAATGGCGTACAATGTAGTGTCAATCCCGATAGCAGCAGGAGTGTTTTTCCCGGTTCTGCGGGTGCAATTACCGCCTTGGGCAGCCGGAGCATGTATGGCACTCTCTTCGGTTAGTGTTGTTTGCTCTTCTTTGCTTCTTAGAAGATACAAGAAGCCAAGACTTACCACTGTTTTGAAAATCACCACGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1001

Amino Acids

107.39

Weight (kDa)

5.06

Isoelectric Point (pI)

34.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	62 - 120	3.4e-13	Heavy-metal-associated domain
HMA	PF00403	137 - 197	1.5e-13	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	440 - 543	6e-25	P-type ATPase actuator domain
Hydrolase	PF00702	652 - 889	5.6e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2753
AasI	GACNNNNNNGTC	1 cut(s)	714
AccB1I	GGYRCC	2 cut(s)	4, 1982
AccBSI	CCGCTC	1 cut(s)	2651
AccI	GTMKAC	2 cut(s)	13, 506
AccII	CGCG	1 cut(s)	2414
AccIII	TCCGGA	2 cut(s)	757, 1777
AciI	CCGC	9 cut(s)	1632, 1667, 2355, 2414, 2436, 2649, 2658, 2872, 2885
AclI	AACGTT	1 cut(s)	240
AclWI	GGATC	6 cut(s)	527, 749, 1426, 1597, 2411, 2424
AcsI	RAATTY	3 cut(s)	1661, 2048, 2330
AcuI	CTGAAG	3 cut(s)	396, 1359, 2172
AcyI	GRCGYC	2 cut(s)	5, 249
AdeI	CACNNNGTG	1 cut(s)	1954
AfaI	GTAC	4 cut(s)	1305, 1543, 2117, 2822
AfiI	CCNNNNNNNGG	9 cut(s)	41, 165, 1442, 1535, 1784, 2234, 2235, 2385, 2464
AgeI	ACCGGT	1 cut(s)	188
AhlI	ACTAGT	1 cut(s)	1300
AjiI	CACGTC	1 cut(s)	1134
AjnI	CCWGG	2 cut(s)	475, 1396
AjuI	GAANNNNNNNTTGG	4 cut(s)	792, 824, 1010, 1042
Alw21I	GWGCWC	4 cut(s)	1087, 1119, 1564, 1852
Alw26I	GTCTC	5 cut(s)	1200, 1266, 1622, 2459, 2622
Alw44I	GTGCAC	1 cut(s)	1848
AlwI	GGATC	6 cut(s)	527, 749, 1426, 1597, 2411, 2424
AlwNI	CAGNNNCTG	2 cut(s)	1184, 1505
Aor13HI	TCCGGA	2 cut(s)	757, 1777
AoxI	GGCC	2 cut(s)	873, 1023
ApaLI	GTGCAC	1 cut(s)	1848
ApeKI	GCWGC	9 cut(s)	203, 227, 434, 1073, 2593, 2660, 2711, 2846, 2894
ApoI	RAATTY	3 cut(s)	1661, 2048, 2330
AseI	ATTAAT	1 cut(s)	1548
AsiGI	ACCGGT	1 cut(s)	188
Asp700I	GAANNNNTTC	4 cut(s)	459, 1096, 1500, 1773
AspLEI	GCGC	2 cut(s)	7, 1220
AspS9I	GGNCC	3 cut(s)	992, 1023, 2038
AsuC2I	CCSGG	3 cut(s)	1422, 2229, 2864
AsuII	TTCGAA	1 cut(s)	857
AsuNHI	GCTAGC	1 cut(s)	2078
AvaII	GGWCC	2 cut(s)	992, 2038
BaeGI	GKGCMC	2 cut(s)	1133, 1852
BamHI	GGATCC	1 cut(s)	2416
BanI	GGYRCC	2 cut(s)	4, 1982
BanII	GRGCYC	3 cut(s)	839, 1087, 1165
BbsI	GAAGAC	7 cut(s)	77, 244, 289, 846, 2017, 2751, 2800
Bbv12I	GWGCWC	4 cut(s)	1087, 1119, 1564, 1852
BbvCI	CCTCAGC	1 cut(s)	2073
BbvI	GCAGC	9 cut(s)	190, 239, 446, 1085, 2580, 2647, 2723, 2858, 2906
BcgI	CGANNNNNNTGC	2 cut(s)	2901, 2935
BciT130I	CCWGG	2 cut(s)	477, 1398
BciVI	GTATCC	1 cut(s)	675
BclI	TGATCA	1 cut(s)	1917
BcnI	CCSGG	3 cut(s)	1422, 2229, 2864
BcoDI	GTCTC	5 cut(s)	1200, 1266, 1622, 2459, 2622
BcuI	ACTAGT	1 cut(s)	1300
BfaI	CTAG	4 cut(s)	690, 1301, 2079, 2654
BfmI	CTRYAG	4 cut(s)	339, 1437, 2106, 2661
BfoI	RGCGCY	2 cut(s)	8, 1221
BfuI	GTATCC	1 cut(s)	675
BglII	AGATCT	3 cut(s)	469, 776, 2341
Bme1390I	CCNGG	5 cut(s)	477, 1398, 1422, 2229, 2864
Bme18I	GGWCC	2 cut(s)	992, 2038
BmgBI	CACGTC	1 cut(s)	1134
BmgT120I	GGNCC	3 cut(s)	992, 1023, 2038
BmiI	GGNNCC	4 cut(s)	6, 994, 1984, 2418
BmrFI	CCNGG	5 cut(s)	477, 1398, 1422, 2229, 2864
BmrI	ACTGGG	1 cut(s)	1253
BmsI	GCATC	9 cut(s)	337, 568, 652, 958, 1201, 1276, 1369, 1585, 1921
BmtI	GCTAGC	1 cut(s)	2082
BmuI	ACTGGG	1 cut(s)	1253
BoxI	GACNNNNGTC	1 cut(s)	2366
BpiI	GAAGAC	7 cut(s)	77, 244, 289, 846, 2017, 2751, 2800
BpmI	CTGGAG	5 cut(s)	498, 753, 886, 888, 2521
Bpu10I	CCTNAGC	2 cut(s)	666, 2073
Bpu14I	TTCGAA	1 cut(s)	857
BpuEI	CTTGAG	2 cut(s)	761, 1977
BpuMI	CCSGG	3 cut(s)	1422, 2229, 2864
Bsa29I	ATCGAT	1 cut(s)	2763
BsaBI	GATNNNNATC	2 cut(s)	96, 2408
BsaHI	GRCGYC	2 cut(s)	5, 249
BsaI	GGTCTC	3 cut(s)	1200, 1622, 2459
BsaJI	CCNNGG	7 cut(s)	1026, 1281, 2041, 2208, 2619, 2888, 2997
BsaWI	WCCGGW	7 cut(s)	34, 41, 158, 188, 757, 1693, 1777
BsaXI	ACNNNNNCTCC	2 cut(s)	2358, 2388
Bsc4I	CCNNNNNNNGG	9 cut(s)	41, 165, 1442, 1535, 1784, 2234, 2235, 2385, 2464
Bse118I	RCCGGY	1 cut(s)	188
Bse1I	ACTGG	8 cut(s)	871, 903, 981, 1189, 1248, 1495, 1529, 2504
Bse3DI	GCAATG	2 cut(s)	954, 2820
Bse8I	GATNNNNATC	2 cut(s)	96, 2408
BseAI	TCCGGA	2 cut(s)	757, 1777
BseBI	CCWGG	2 cut(s)	477, 1398
BseCI	ATCGAT	1 cut(s)	2763
BseDI	CCNNGG	7 cut(s)	1026, 1281, 2041, 2208, 2619, 2888, 2997
BseGI	GGATG	9 cut(s)	352, 559, 646, 933, 1117, 1291, 1417, 2198, 2310
BseJI	GATNNNNATC	2 cut(s)	96, 2408
BseLI	CCNNNNNNNGG	9 cut(s)	41, 165, 1442, 1535, 1784, 2234, 2235, 2385, 2464
BseMI	GCAATG	2 cut(s)	954, 2820
BseMII	CTCAG	5 cut(s)	354, 680, 1595, 2043, 2064
BseNI	ACTGG	8 cut(s)	871, 903, 981, 1189, 1248, 1495, 1529, 2504
BseSI	GKGCMC	2 cut(s)	1133, 1852
BseXI	GCAGC	9 cut(s)	190, 239, 446, 1085, 2580, 2647, 2723, 2858, 2906
BseYI	CCCAGC	1 cut(s)	581
BsgI	GTGCAG	3 cut(s)	453, 623, 2706
Bsh1236I	CGCG	1 cut(s)	2414
BshFI	GGCC	2 cut(s)	875, 1025
BshNI	GGYRCC	2 cut(s)	4, 1982
BshTI	ACCGGT	1 cut(s)	188
BshVI	ATCGAT	1 cut(s)	2763
BsiHKAI	GWGCWC	4 cut(s)	1087, 1119, 1564, 1852
BsiWI	CGTACG	1 cut(s)	2115
BslFI	GGGAC	1 cut(s)	1801
BslI	CCNNNNNNNGG	9 cut(s)	41, 165, 1442, 1535, 1784, 2234, 2235, 2385, 2464
BsmAI	GTCTC	5 cut(s)	1200, 1266, 1622, 2459, 2622
BsmBI	CGTCTC	1 cut(s)	2622
BsmFI	GGGAC	1 cut(s)	1801
BsnI	GGCC	2 cut(s)	875, 1025
Bso31I	GGTCTC	3 cut(s)	1200, 1622, 2459
Bsp119I	TTCGAA	1 cut(s)	857
Bsp1286I	GDGCHC	7 cut(s)	839, 1087, 1119, 1133, 1165, 1564, 1852
Bsp13I	TCCGGA	2 cut(s)	757, 1777
Bsp19I	CCATGG	1 cut(s)	2619
BspACI	CCGC	9 cut(s)	1632, 1667, 2355, 2414, 2436, 2649, 2658, 2872, 2885
BspANI	GGCC	2 cut(s)	875, 1025
BspCNI	CTCAG	5 cut(s)	355, 679, 1596, 2042, 2065
BspDI	ATCGAT	1 cut(s)	2763
BspEI	TCCGGA	2 cut(s)	757, 1777
BspFNI	CGCG	1 cut(s)	2414
BspHI	TCATGA	1 cut(s)	999
BspLI	GGNNCC	4 cut(s)	6, 994, 1984, 2418
BspMAI	CTGCAG	2 cut(s)	1441, 2665
BspOI	GCTAGC	1 cut(s)	2082
BspPI	GGATC	6 cut(s)	527, 749, 1426, 1597, 2411, 2424
BspQI	GCTCTTC	3 cut(s)	1569, 2229, 2944
BspT104I	TTCGAA	1 cut(s)	857
BspT107I	GGYRCC	2 cut(s)	4, 1982
BspTNI	GGTCTC	3 cut(s)	1200, 1622, 2459
BsrBI	CCGCTC	1 cut(s)	2651
BsrDI	GCAATG	2 cut(s)	954, 2820
BsrFI	RCCGGY	1 cut(s)	188
BsrI	ACTGG	8 cut(s)	871, 903, 981, 1189, 1248, 1495, 1529, 2504
BssAI	RCCGGY	1 cut(s)	188
BssECI	CCNNGG	7 cut(s)	1026, 1281, 2041, 2208, 2619, 2888, 2997
BssNI	GRCGYC	2 cut(s)	5, 249
BssT1I	CCWWGG	3 cut(s)	1026, 2619, 2888
Bst2UI	CCWGG	2 cut(s)	477, 1398
Bst4CI	ACNGT	6 cut(s)	711, 2008, 2042, 2282, 2614, 2983
Bst6I	CTCTTC	7 cut(s)	142, 315, 932, 1569, 2229, 2923, 2944
BstACI	GRCGYC	2 cut(s)	5, 249
BstAPI	GCANNNNNTGC	1 cut(s)	2684
BstBI	TTCGAA	1 cut(s)	857
BstC8I	GCNNGC	5 cut(s)	1058, 1216, 1317, 2080, 2565
BstDSI	CCRYGG	3 cut(s)	2041, 2619, 2997
BstEII	GGTNACC	1 cut(s)	37
BstF5I	GGATG	9 cut(s)	352, 559, 646, 933, 1117, 1291, 1417, 2198, 2310
BstFNI	CGCG	1 cut(s)	2414
BstH2I	RGCGCY	2 cut(s)	8, 1221
BstHHI	GCGC	2 cut(s)	7, 1220
BstMAI	GTCTC	5 cut(s)	1200, 1266, 1622, 2459, 2622
BstNI	CCWGG	2 cut(s)	477, 1398
BstNSI	RCATGY	1 cut(s)	2907
BstPAI	GACNNNNGTC	1 cut(s)	2366
BstPI	GGTNACC	1 cut(s)	37
BstSCI	CCNGG	5 cut(s)	475, 1396, 1420, 2227, 2862
BstSFI	CTRYAG	4 cut(s)	339, 1437, 2106, 2661
BstSLI	GKGCMC	2 cut(s)	1133, 1852
BstUI	CGCG	1 cut(s)	2414
BstV1I	GCAGC	9 cut(s)	190, 239, 446, 1085, 2580, 2647, 2723, 2858, 2906
BstV2I	GAAGAC	7 cut(s)	77, 244, 289, 846, 2017, 2751, 2800
BstX2I	RGATCY	6 cut(s)	469, 776, 1431, 1589, 2341, 2416
BstXI	CCANNNNNNTGG	1 cut(s)	1770
BstYI	RGATCY	6 cut(s)	469, 776, 1431, 1589, 2341, 2416
Bsu15I	ATCGAT	1 cut(s)	2763
BsuI	GTATCC	1 cut(s)	675
BsuRI	GGCC	2 cut(s)	875, 1025
BsuTUI	ATCGAT	1 cut(s)	2763
BtgI	CCRYGG	3 cut(s)	2041, 2619, 2997
BtrI	CACGTC	1 cut(s)	1134
BtsCI	GGATG	9 cut(s)	352, 559, 646, 933, 1117, 1291, 1417, 2198, 2310
BtsI	GCAGTG	2 cut(s)	498, 2089
BtsIMutI	CAGTG	7 cut(s)	70, 498, 1182, 2089, 2148, 2249, 2979
Cac8I	GCNNGC	5 cut(s)	1058, 1216, 1317, 2080, 2565
CaiI	CAGNNNCTG	2 cut(s)	1184, 1505
CciI	TCATGA	1 cut(s)	999
CfoI	GCGC	2 cut(s)	7, 1220
Cfr10I	RCCGGY	1 cut(s)	188
Cfr13I	GGNCC	3 cut(s)	992, 1023, 2038
ClaI	ATCGAT	1 cut(s)	2763
CseI	GACGC	2 cut(s)	238, 2055
Csp6I	GTAC	4 cut(s)	1304, 1542, 2116, 2821
CspAI	ACCGGT	1 cut(s)	188
CviAII	CATG	9 cut(s)	1000, 1077, 1108, 1222, 1556, 1570, 2321, 2620, 2904
CviQI	GTAC	4 cut(s)	1304, 1542, 2116, 2821
DinI	GGCGCC	1 cut(s)	6
DraIII	CACNNNGTG	1 cut(s)	1954
DrdI	GACNNNNNNGTC	1 cut(s)	714
DseDI	GACNNNNNNGTC	1 cut(s)	714
Eam1104I	CTCTTC	7 cut(s)	142, 315, 932, 1569, 2229, 2923, 2944
EarI	CTCTTC	7 cut(s)	142, 315, 932, 1569, 2229, 2923, 2944
Ecl136II	GAGCTC	1 cut(s)	1085
Eco130I	CCWWGG	3 cut(s)	1026, 2619, 2888
Eco147I	AGGCCT	1 cut(s)	875
Eco24I	GRGCYC	3 cut(s)	839, 1087, 1165
Eco31I	GGTCTC	3 cut(s)	1200, 1622, 2459
Eco32I	GATATC	1 cut(s)	729
Eco47I	GGWCC	2 cut(s)	992, 2038
Eco53kI	GAGCTC	1 cut(s)	1085
Eco57I	CTGAAG	3 cut(s)	396, 1359, 2172
Eco91I	GGTNACC	1 cut(s)	37
EcoICRI	GAGCTC	1 cut(s)	1085
EcoO65I	GGTNACC	1 cut(s)	37
EcoRI	GAATTC	1 cut(s)	2048
EcoRII	CCWGG	2 cut(s)	475, 1396
EcoRV	GATATC	1 cut(s)	729
EcoT14I	CCWWGG	3 cut(s)	1026, 2619, 2888
EcoT22I	ATGCAT	3 cut(s)	1384, 1557, 1936
EcoT38I	GRGCYC	3 cut(s)	839, 1087, 1165
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	1026, 2619, 2888
Esp3I	CGTCTC	1 cut(s)	2622
FaeI	CATG	9 cut(s)	1003, 1080, 1111, 1225, 1559, 1573, 2324, 2623, 2907
FaqI	GGGAC	1 cut(s)	1801
FatI	CATG	9 cut(s)	999, 1076, 1107, 1221, 1555, 1569, 2320, 2619, 2903
FauI	CCCGC	2 cut(s)	2656, 2865
FbaI	TGATCA	1 cut(s)	1917
FblI	GTMKAC	2 cut(s)	13, 506
FokI	GGATG	9 cut(s)	359, 566, 653, 920, 1124, 1298, 1404, 2205, 2297
FriOI	GRGCYC	3 cut(s)	839, 1087, 1165
FspBI	CTAG	4 cut(s)	690, 1301, 2079, 2654
GlaI	GCGC	2 cut(s)	6, 1219
GsaI	CCCAGC	1 cut(s)	585
GsuI	CTGGAG	5 cut(s)	498, 753, 886, 888, 2521
HaeII	RGCGCY	2 cut(s)	8, 1221
HaeIII	GGCC	2 cut(s)	875, 1025
HgaI	GACGC	2 cut(s)	238, 2055
HhaI	GCGC	2 cut(s)	7, 1220
Hin1I	GRCGYC	2 cut(s)	5, 249
Hin1II	CATG	9 cut(s)	1003, 1080, 1111, 1225, 1559, 1573, 2324, 2623, 2907
Hin6I	GCGC	2 cut(s)	5, 1218
HinP1I	GCGC	2 cut(s)	5, 1218
HincII	GTYRAC	1 cut(s)	445
HindII	GTYRAC	1 cut(s)	445
HindIII	AAGCTT	2 cut(s)	623, 1355
HpaI	GTTAAC	1 cut(s)	445
Hpy166II	GTNNAC	8 cut(s)	14, 445, 507, 992, 1719, 1850, 2087, 2285
Hpy8I	GTNNAC	8 cut(s)	14, 445, 507, 992, 1719, 1850, 2087, 2285
Hpy99I	CGWCG	1 cut(s)	1124
HpyAV	CCTTC	7 cut(s)	137, 449, 584, 671, 1006, 1334, 2444
HpyCH4III	ACNGT	6 cut(s)	711, 2008, 2042, 2282, 2614, 2983
HpyCH4IV	ACGT	8 cut(s)	240, 1133, 1470, 2580, 2587, 2721, 2748, 2805
HpySE526I	ACGT	8 cut(s)	240, 1133, 1470, 2580, 2587, 2721, 2748, 2805
Hsp92I	GRCGYC	2 cut(s)	5, 249
Hsp92II	CATG	9 cut(s)	1003, 1080, 1111, 1225, 1559, 1573, 2324, 2623, 2907
HspAI	GCGC	2 cut(s)	5, 1218
KasI	GGCGCC	1 cut(s)	4
Kpn2I	TCCGGA	2 cut(s)	757, 1777
Ksp22I	TGATCA	1 cut(s)	1917
KspAI	GTTAAC	1 cut(s)	445
LguI	GCTCTTC	3 cut(s)	1569, 2229, 2944
LmnI	GCTCC	6 cut(s)	1175, 1654, 1745, 1754, 1898, 2900
Lsp1109I	GCAGC	9 cut(s)	190, 239, 446, 1085, 2580, 2647, 2723, 2858, 2906
LweI	GCATC	9 cut(s)	337, 568, 652, 958, 1201, 1276, 1369, 1585, 1921
MaeI	CTAG	4 cut(s)	690, 1301, 2079, 2654
MaeII	ACGT	8 cut(s)	240, 1133, 1470, 2580, 2587, 2721, 2748, 2805
MaeIII	GTNAC	9 cut(s)	37, 184, 273, 1180, 1466, 1486, 2008, 2196, 2485
MbiI	CCGCTC	1 cut(s)	2651
MfeI	CAATTG	3 cut(s)	414, 1737, 2679
MflI	RGATCY	6 cut(s)	469, 776, 1431, 1589, 2341, 2416
MhlI	GDGCHC	7 cut(s)	839, 1087, 1119, 1133, 1165, 1564, 1852
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	192, 1268, 1783, 2376, 2636
MmeI	TCCRAC	4 cut(s)	160, 1223, 1732, 1876
Mph1103I	ATGCAT	3 cut(s)	1384, 1557, 1936
MroI	TCCGGA	2 cut(s)	757, 1777
MroXI	GAANNNNTTC	4 cut(s)	459, 1096, 1500, 1773
MslI	CAYNNNNRTG	3 cut(s)	191, 1112, 1422
MspA1I	CMGCKG	2 cut(s)	2567, 2660
MspR9I	CCNGG	5 cut(s)	477, 1398, 1422, 2229, 2864
MunI	CAATTG	3 cut(s)	414, 1737, 2679
MvaI	CCWGG	2 cut(s)	477, 1398
MvnI	CGCG	1 cut(s)	2414
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	3 cut(s)	1422, 2229, 2864
NcoI	CCATGG	1 cut(s)	2619
NheI	GCTAGC	1 cut(s)	2078
NlaIII	CATG	9 cut(s)	1003, 1080, 1111, 1225, 1559, 1573, 2324, 2623, 2907
NlaIV	GGNNCC	4 cut(s)	6, 994, 1984, 2418
NmeAIII	GCCGAG	2 cut(s)	33, 2101
NmuCI	GTSAC	5 cut(s)	37, 184, 1180, 1486, 2008
NsiI	ATGCAT	3 cut(s)	1384, 1557, 1936
NspI	RCATGY	1 cut(s)	2907
NspV	TTCGAA	1 cut(s)	857
PagI	TCATGA	1 cut(s)	999
PceI	AGGCCT	1 cut(s)	875
PciSI	GCTCTTC	3 cut(s)	1569, 2229, 2944
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PdmI	GAANNNNTTC	4 cut(s)	459, 1096, 1500, 1773
Pfl23II	CGTACG	1 cut(s)	2115
PflFI	GACNNNGTC	1 cut(s)	2321
PfoI	TCCNGGA	2 cut(s)	475, 2227
PinAI	ACCGGT	1 cut(s)	188
PleI	GAGTC	5 cut(s)	191, 1267, 1783, 2375, 2636
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	5 cut(s)	191, 1267, 1783, 2375, 2636
PshAI	GACNNNNGTC	1 cut(s)	2366
PshBI	ATTAAT	1 cut(s)	1548
PsiI	TTATAA	1 cut(s)	2753
Psp124BI	GAGCTC	1 cut(s)	1087
Psp1406I	AACGTT	1 cut(s)	240
Psp6I	CCWGG	2 cut(s)	475, 1396
PspEI	GGTNACC	1 cut(s)	37
PspFI	CCCAGC	1 cut(s)	581
PspGI	CCWGG	2 cut(s)	475, 1396
PspLI	CGTACG	1 cut(s)	2115
PspN4I	GGNNCC	4 cut(s)	6, 994, 1984, 2418
PspPI	GGNCC	3 cut(s)	992, 1023, 2038
PstI	CTGCAG	2 cut(s)	1441, 2665
PstNI	CAGNNNCTG	2 cut(s)	1184, 1505
PsuI	RGATCY	6 cut(s)	469, 776, 1431, 1589, 2341, 2416
PsyI	GACNNNGTC	1 cut(s)	2321
PvuII	CAGCTG	1 cut(s)	2567
RsaI	GTAC	4 cut(s)	1305, 1543, 2117, 2822
RsaNI	GTAC	4 cut(s)	1304, 1542, 2116, 2821
RseI	CAYNNNNRTG	3 cut(s)	191, 1112, 1422
SacI	GAGCTC	1 cut(s)	1087
SapI	GCTCTTC	3 cut(s)	1569, 2229, 2944
Sau96I	GGNCC	3 cut(s)	992, 1023, 2038
SchI	GAGTC	5 cut(s)	192, 1268, 1783, 2376, 2636
ScrFI	CCNGG	5 cut(s)	477, 1398, 1422, 2229, 2864
SduI	GDGCHC	7 cut(s)	839, 1087, 1119, 1133, 1165, 1564, 1852
SfaNI	GCATC	9 cut(s)	337, 568, 652, 958, 1201, 1276, 1369, 1585, 1921
SfcI	CTRYAG	4 cut(s)	339, 1437, 2106, 2661
SfoI	GGCGCC	1 cut(s)	6
SfuI	TTCGAA	1 cut(s)	857
SinI	GGWCC	2 cut(s)	992, 2038
SmiMI	CAYNNNNRTG	3 cut(s)	191, 1112, 1422
SmlI	CTYRAG	2 cut(s)	740, 1992
SmoI	CTYRAG	2 cut(s)	740, 1992
SpeI	ACTAGT	1 cut(s)	1300
SseBI	AGGCCT	1 cut(s)	875
SsiI	CCGC	9 cut(s)	1632, 1667, 2355, 2414, 2436, 2649, 2658, 2872, 2885
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	1253
SspMI	CTAG	4 cut(s)	690, 1301, 2079, 2654
SstI	GAGCTC	1 cut(s)	1087
StuI	AGGCCT	1 cut(s)	875
StyD4I	CCNGG	5 cut(s)	475, 1396, 1420, 2227, 2862
StyI	CCWWGG	3 cut(s)	1026, 2619, 2888
TaaI	ACNGT	6 cut(s)	711, 2008, 2042, 2282, 2614, 2983
TaiI	ACGT	8 cut(s)	243, 1136, 1473, 2583, 2590, 2724, 2751, 2808
TatI	WGTACW	1 cut(s)	1303
TauI	GCSGC	2 cut(s)	2438, 2660
TscAI	CASTG	7 cut(s)	70, 498, 1189, 2089, 2155, 2249, 2986
TseFI	GTSAC	5 cut(s)	37, 184, 1180, 1486, 2008
TseI	GCWGC	9 cut(s)	203, 227, 434, 1073, 2593, 2660, 2711, 2846, 2894
Tsp45I	GTSAC	5 cut(s)	37, 184, 1180, 1486, 2008
TspDTI	ATGAA	9 cut(s)	251, 858, 922, 988, 1307, 1406, 1491, 1586, 2157
TspGWI	ACGGA	4 cut(s)	208, 1813, 2619, 2646
TspRI	CASTG	7 cut(s)	70, 498, 1189, 2089, 2155, 2249, 2986
Tth111I	GACNNNGTC	1 cut(s)	2321
VneI	GTGCAC	1 cut(s)	1848
VpaK11BI	GGWCC	2 cut(s)	992, 2038
VspI	ATTAAT	1 cut(s)	1548
XapI	RAATTY	3 cut(s)	1661, 2048, 2330
XceI	RCATGY	1 cut(s)	2907
XmiI	GTMKAC	2 cut(s)	13, 506
XmnI	GAANNNNTTC	4 cut(s)	459, 1096, 1500, 1773
XspI	CTAG	4 cut(s)	690, 1301, 2079, 2654
Zsp2I	ATGCAT	3 cut(s)	1384, 1557, 1936

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.