Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

15984657 .. 15991447

Gene Structure 6791 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG152200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCGCCGAGTCTCAGGGACCTCCAGCTCACGCAGGTCTGGAACTCCGGCCGCGGCGGCGGCGACGGCGGCGGCGGCGAAGACCTCGAGGACGTGAGGCTTTTGGATTCGTATGAGAATTCGGAGGGAGTTGAAGAAGGGATGAGGACGGTTCAGGTTAGAGTTACCGGGATGACGTGTGCTGCTTGCTCTAATTCGGTTGAGGGAGCTCTCCAGAGCGTCAATGGCGTGCTCAGGGCCTCCGTGGCTTTGCTTCAGAATCGGGCTGACGTGGTTTTTGATCCCAGATTGGTCAAGGATGAAGATATCAAAAATGCAATTGAAGATGCTGGGTTTGAGGCAGAGGTTATACCTGAGCCCAGTGCAAACGGAGTAAAGCAGCATGGAAACCTGTCCGGTCAGTTTTCGATTGGAGGTATGACATGTGCAGCTTGCGTGAACTCTGTTGAAGGTATTCTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGGGAAGTGGAGTATGATCCTACTGTAATTAGTAAAGATGACATAGTCAATGCCATTGAAGATGCTGGTTTTGAAGCATCTTTTGTACAGAGTAGTGAGCAGGATAAAATTATACTGGGAGTTGCTGGCATATTCAGTGAGATAGATGCACAGGTTTTAGAAGCCATAATCAGCAACTTGAAAGGGGTGAGACATTTTCGTCTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCAATGGGAAGTTCAAACTTCAAGTAGCAAACCCGTATACAAGAATGACTTGCAAAGATGTTGATGAAGCCGCAAATATGTTTCGGCTTTTCATTTCCAGCCTGGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTAGTCTGCCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGGCCTTTCGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTGATTGGAAAGCGCTTCTACATTGCAGCTGCAAGAGCCCTACGAAATGGTTCAACTAACATGGATGTTCTGGTCGCCTTGGGAACTTCGGCCTCTTACTTCTACTCTGTTTGTGCACTTCTATATGGTGCAATCACTGGGTTTTGGTCTCCGACATACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTATTGGGGAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCCATCAAAAAGTTGATAGAACTCACTCCAGCTACAGCTTTGTTGCTTGTTAAAGATAAAGGTGGGAGATGTGTTGGAGAGAGGGAAATTGATGCTTTGCTCATTCAGCCTGGAGATACGTTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGATTGTGTGGGGTTCAAGTTATGTAAATGAGAGTATGGTAACTGGGGAAGCTATACCTGTTTCAAAAGAAGTCAACACGTTGGTGATTGGAGGAACAATAAATTTACATGGTGCCCTTCACGTACAAGTTACAAAAGTTGGATCTGATACAGTTTTACATCAAATAATTAATTTGGTCGAGACTGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGTATTGTCATTGTTGACATTTTTGGGTTGGTATGTTGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCTGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATATCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACAGCTGTCATGGTCGCAACAGGGGTTGGTGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTTTGGAAAGAGCTCAAAAGGTTAAGTACGTGATATTTGATAAAACAGGCACCCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAGTTTTCACAGGAATGGATCGTGGAGATTTTCTTAAGTTGGTGGCATCTGCAGAGGCTAGCAGTGAACACCCACTAGGAAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAGCCTTCTGCAAACAATGATGCAACAAACCAAAGCAAGGAGCCCCTGATCTCTGGATGGCTTTTTGATGTCTCAGATTTCTCTGCTTTGCCAGGTAGAGGCATCCAGTGCTCAATAAATGGAAAACTTATTCTGGTTGGTAATAGGAAATTGATGACTGAAAGCGGAATCGACATTCCAACCCATGTTGAGGACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATCCTTGTTGCATATGAAGGCAATTTAGTTGGTGTATTGGGGGTTGCCGACCCACTAAAAAGAGAAGCTGCAATAGTCATAGAGGGACTTGGTAAGATGGGTGTCATACCAGTCATGGTTACAGGAGATAATTGGAGGACAGCACAGGCTGTTGCAAAGGAGGTTTGTATTTATCTCTCTATACCTTCAGTTTTTCACTACGCTGTTGCTGAACTTTATAACTATTCTCTTTCTTGCAACCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

870

Amino Acids

93.31

Weight (kDa)

5.12

Isoelectric Point (pI)

27.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	53 - 113	5e-13	Heavy-metal-associated domain
HMA	PF00403	135 - 195	1.9e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	436 - 541	1.4e-24	P-type ATPase actuator domain
Hydrolase	PF00702	650 - 844	3.4e-16	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2586
AasI	GACNNNNNNGTC	1 cut(s)	708
AbsI	CCTCGAGG	1 cut(s)	86
Acc36I	ACCTGC	1 cut(s)	25
AccB1I	GGYRCC	3 cut(s)	4, 1553, 1976
AccI	GTMKAC	1 cut(s)	836
AccII	CGCG	1 cut(s)	54
AciI	CCGC	9 cut(s)	52, 54, 57, 60, 69, 72, 75, 870, 2301
AclWI	GGATC	6 cut(s)	275, 521, 743, 765, 1591, 2040
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	3 cut(s)	118, 1543, 1655
AcuI	CTGAAG	3 cut(s)	239, 774, 2538
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	5 cut(s)	597, 1233, 1420, 1566, 1955
AfeI	AGCGCT	1 cut(s)	1055
AfiI	CCNNNNNNNGG	6 cut(s)	1436, 1778, 1993, 2173, 2234, 2326
AflII	CTTAAG	1 cut(s)	2048
AflIII	ACRYGT	3 cut(s)	176, 422, 1518
AjiI	CACGTC	3 cut(s)	94, 177, 271
AjnI	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
AjuI	GAANNNNNNNTTGG	2 cut(s)	1004, 1036
AleI	CACNNNNGTG	1 cut(s)	1523
AloI	GAACNNNNNNTCC	4 cut(s)	723, 755, 1752, 1784
Alw21I	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
Alw26I	GTCTC	5 cut(s)	17, 694, 1194, 1616, 2212
Alw44I	GTGCAC	2 cut(s)	1155, 1842
AlwI	GGATC	6 cut(s)	275, 521, 743, 765, 1591, 2040
AlwNI	CAGNNNCTG	1 cut(s)	2006
Ama87I	CYCGRG	1 cut(s)	86
Aor51HI	AGCGCT	1 cut(s)	1055
AoxI	GGCC	4 cut(s)	49, 237, 986, 1131
ApaLI	GTGCAC	2 cut(s)	1155, 1842
ApeKI	GCWGC	6 cut(s)	182, 379, 428, 1067, 1070, 2435
ApoI	RAATTY	3 cut(s)	118, 1543, 1655
ArsI	GACNNNNNNTTYG	2 cut(s)	260, 292
AseI	ATTAAT	1 cut(s)	1611
Asp700I	GAANNNNTTC	2 cut(s)	453, 1090
AspLEI	GCGC	2 cut(s)	7, 1056
AspS9I	GGNCC	3 cut(s)	19, 237, 986
AsuC2I	CCSGG	1 cut(s)	169
AsuHPI	GGTGA	4 cut(s)	709, 1013, 1036, 1537
AsuII	TTCGAA	1 cut(s)	993
AsuNHI	GCTAGC	2 cut(s)	797, 2072
AvaI	CYCGRG	1 cut(s)	86
AvaII	GGWCC	1 cut(s)	19
BaeGI	GKGCMC	3 cut(s)	1159, 1558, 1846
BaeI	ACNNNNGTAYC	2 cut(s)	1410, 1443
BanI	GGYRCC	3 cut(s)	4, 1553, 1976
BanII	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
BbsI	GAAGAC	1 cut(s)	87
Bbv12I	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
BbvI	GCAGC	6 cut(s)	169, 391, 440, 1057, 1079, 2422
BccI	CCATC	5 cut(s)	776, 1292, 1430, 2187, 2458
BceAI	ACGGC	1 cut(s)	82
BcgI	CGANNNNNNTGC	2 cut(s)	921, 955
BciT130I	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BcnI	CCSGG	1 cut(s)	169
BcoDI	GTCTC	5 cut(s)	17, 694, 1194, 1616, 2212
BfaI	CTAG	5 cut(s)	509, 768, 798, 2073, 2090
BfmI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
BfoI	RGCGCY	2 cut(s)	8, 1057
BfrI	CTTAAG	1 cut(s)	2048
BfuAI	ACCTGC	1 cut(s)	25
BglI	GCCNNNNNGGC	2 cut(s)	57, 245
Bme1390I	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
Bme18I	GGWCC	1 cut(s)	19
BmeT110I	CYCGRG	1 cut(s)	86
BmgBI	CACGTC	3 cut(s)	94, 177, 271
BmgT120I	GGNCC	3 cut(s)	19, 237, 986
BmiI	GGNNCC	7 cut(s)	6, 20, 1555, 1741, 1769, 1978, 2178
BmrFI	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
BmrI	ACTGGG	4 cut(s)	354, 635, 1188, 1494
BmtI	GCTAGC	2 cut(s)	801, 2076
BmuI	ACTGGG	4 cut(s)	354, 635, 1188, 1494
BpiI	GAAGAC	1 cut(s)	87
BpmI	CTGGAG	6 cut(s)	8, 197, 997, 1293, 1413, 1758
Bpu10I	CCTNAGC	2 cut(s)	233, 354
Bpu14I	TTCGAA	1 cut(s)	993
BpuMI	CCSGG	1 cut(s)	169
BsaAI	YACGTR	2 cut(s)	1564, 1957
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	1194
BsaJI	CCNNGG	6 cut(s)	52, 243, 470, 774, 1119, 1987
BsaWI	WCCGGW	1 cut(s)	395
BsaXI	ACNNNNNCTCC	2 cut(s)	1752, 1782
Bsc4I	CCNNNNNNNGG	6 cut(s)	1436, 1778, 1993, 2173, 2234, 2326
Bse1I	ACTGG	6 cut(s)	360, 630, 1183, 1489, 2242, 2477
Bse3DI	GCAATG	3 cut(s)	808, 1062, 1827
BseBI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BseDI	CCNNGG	6 cut(s)	52, 243, 470, 774, 1119, 1987
BseGI	GGATG	7 cut(s)	147, 177, 304, 1111, 2198, 2238, 2367
BseLI	CCNNNNNNNGG	6 cut(s)	1436, 1778, 1993, 2173, 2234, 2326
BseMI	GCAATG	3 cut(s)	808, 1062, 1827
BseMII	CTCAG	5 cut(s)	28, 247, 345, 922, 2223
BseNI	ACTGG	6 cut(s)	360, 630, 1183, 1489, 2242, 2477
BseRI	GAGGAG	1 cut(s)	1935
BseSI	GKGCMC	3 cut(s)	1159, 1558, 1846
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	6 cut(s)	169, 391, 440, 1057, 1079, 2422
BseYI	CCCAGC	1 cut(s)	329
BsgI	GTGCAG	1 cut(s)	447
Bsh1236I	CGCG	1 cut(s)	54
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	4 cut(s)	51, 239, 988, 1133
BshNI	GGYRCC	3 cut(s)	4, 1553, 1976
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
BsiHKCI	CYCGRG	1 cut(s)	86
BsiSI	CCGG	3 cut(s)	48, 168, 396
BslFI	GGGAC	2 cut(s)	32, 2466
BslI	CCNNNNNNNGG	6 cut(s)	1436, 1778, 1993, 2173, 2234, 2326
BsmAI	GTCTC	5 cut(s)	17, 694, 1194, 1616, 2212
BsmFI	GGGAC	2 cut(s)	32, 2466
BsnI	GGCC	4 cut(s)	51, 239, 988, 1133
Bso31I	GGTCTC	1 cut(s)	1194
BsoBI	CYCGRG	1 cut(s)	86
Bsp119I	TTCGAA	1 cut(s)	993
Bsp1407I	TGTACA	1 cut(s)	595
Bsp143I	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
BspACI	CCGC	9 cut(s)	52, 54, 57, 60, 69, 72, 75, 870, 2301
BspANI	GGCC	4 cut(s)	51, 239, 988, 1133
BspCNI	CTCAG	5 cut(s)	27, 246, 346, 921, 2222
BspFNI	CGCG	1 cut(s)	54
BspLI	GGNNCC	7 cut(s)	6, 20, 1555, 1741, 1769, 1978, 2178
BspMAI	CTGCAG	1 cut(s)	2068
BspMI	ACCTGC	1 cut(s)	25
BspOI	GCTAGC	2 cut(s)	801, 2076
BspPI	GGATC	6 cut(s)	275, 521, 743, 765, 1591, 2040
BspT104I	TTCGAA	1 cut(s)	993
BspT107I	GGYRCC	3 cut(s)	4, 1553, 1976
BspTI	CTTAAG	1 cut(s)	2048
BspTNI	GGTCTC	1 cut(s)	1194
BsrDI	GCAATG	3 cut(s)	808, 1062, 1827
BsrGI	TGTACA	1 cut(s)	595
BsrI	ACTGG	6 cut(s)	360, 630, 1183, 1489, 2242, 2477
BssECI	CCNNGG	6 cut(s)	52, 243, 470, 774, 1119, 1987
BssMI	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
BssNAI	GTATAC	1 cut(s)	837
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	3 cut(s)	774, 1119, 1987
Bst1107I	GTATAC	1 cut(s)	837
Bst2UI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
Bst4CI	ACNGT	6 cut(s)	151, 535, 1594, 1693, 1764, 2002
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2048
BstAPI	GCANNNNNTGC	1 cut(s)	2244
BstAUI	TGTACA	1 cut(s)	595
BstBAI	YACGTR	2 cut(s)	1564, 1957
BstBI	TTCGAA	1 cut(s)	993
BstC8I	GCNNGC	9 cut(s)	187, 230, 433, 637, 799, 1675, 1834, 2074, 2116
BstDEI	CTNAG	5 cut(s)	14, 233, 354, 908, 2209
BstDSI	CCRYGG	2 cut(s)	52, 243
BstF5I	GGATG	7 cut(s)	147, 177, 304, 1111, 2198, 2238, 2367
BstFNI	CGCG	1 cut(s)	54
BstH2I	RGCGCY	2 cut(s)	8, 1057
BstHHI	GCGC	2 cut(s)	7, 1056
BstKTI	GATC	7 cut(s)	283, 529, 751, 773, 1586, 2035, 2187
BstMAI	GTCTC	5 cut(s)	17, 694, 1194, 1616, 2212
BstMBI	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
BstMCI	CGRYCG	1 cut(s)	52
BstNI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BstNSI	RCATGY	2 cut(s)	426, 1836
BstSCI	CCNGG	6 cut(s)	167, 469, 900, 1390, 1414, 2227
BstSFI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
BstSLI	GKGCMC	3 cut(s)	1159, 1558, 1846
BstUI	CGCG	1 cut(s)	54
BstV1I	GCAGC	6 cut(s)	169, 391, 440, 1057, 1079, 2422
BstV2I	GAAGAC	1 cut(s)	87
BstX2I	RGATCY	2 cut(s)	770, 1583
BstXI	CCANNNNNNTGG	1 cut(s)	1764
BstYI	RGATCY	2 cut(s)	770, 1583
BstZ17I	GTATAC	1 cut(s)	837
BstZI	CGGCCG	1 cut(s)	49
BsuRI	GGCC	4 cut(s)	51, 239, 988, 1133
BtgI	CCRYGG	2 cut(s)	52, 243
BtrI	CACGTC	3 cut(s)	94, 177, 271
BtsCI	GGATG	7 cut(s)	147, 177, 304, 1111, 2198, 2238, 2367
BtsI	GCAGTG	1 cut(s)	2083
BtsIMutI	CAGTG	7 cut(s)	367, 652, 916, 1176, 1769, 2083, 2249
BveI	ACCTGC	1 cut(s)	25
Cac8I	GCNNGC	9 cut(s)	187, 230, 433, 637, 799, 1675, 1834, 2074, 2116
CaiI	CAGNNNCTG	1 cut(s)	2006
CfoI	GCGC	2 cut(s)	7, 1056
Cfr13I	GGNCC	3 cut(s)	19, 237, 986
Cfr42I	CCGCGG	1 cut(s)	55
CseI	GACGC	1 cut(s)	208
Csp6I	GTAC	5 cut(s)	596, 1232, 1419, 1565, 1954
CviAII	CATG	9 cut(s)	383, 423, 1102, 1216, 1550, 1833, 1873, 2321, 2482
CviQI	GTAC	5 cut(s)	596, 1232, 1419, 1565, 1954
DdeI	CTNAG	5 cut(s)	14, 233, 354, 908, 2209
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	7 cut(s)	282, 528, 750, 772, 1585, 2034, 2186
DpnII	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
DrdI	GACNNNNNNGTC	1 cut(s)	708
DseDI	GACNNNNNNGTC	1 cut(s)	708
EaeI	YGGCCR	1 cut(s)	49
EagI	CGGCCG	1 cut(s)	49
Ecl136II	GAGCTC	2 cut(s)	209, 1940
EclXI	CGGCCG	1 cut(s)	49
Eco130I	CCWWGG	3 cut(s)	774, 1119, 1987
Eco24I	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
Eco31I	GGTCTC	1 cut(s)	1194
Eco32I	GATATC	1 cut(s)	307
Eco47I	GGWCC	1 cut(s)	19
Eco47III	AGCGCT	1 cut(s)	1055
Eco52I	CGGCCG	1 cut(s)	49
Eco53kI	GAGCTC	2 cut(s)	209, 1940
Eco57I	CTGAAG	3 cut(s)	239, 774, 2538
Eco88I	CYCGRG	1 cut(s)	86
EcoICRI	GAGCTC	2 cut(s)	209, 1940
EcoO109I	RGGNCCY	2 cut(s)	19, 237
EcoRI	GAATTC	1 cut(s)	118
EcoRII	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
EcoRV	GATATC	1 cut(s)	307
EcoT14I	CCWWGG	3 cut(s)	774, 1119, 1987
EcoT38I	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	774, 1119, 1987
FaeI	CATG	9 cut(s)	386, 426, 1105, 1219, 1553, 1836, 1876, 2324, 2485
FalI	AAGNNNNNCTT	2 cut(s)	832, 864
FaqI	GGGAC	2 cut(s)	32, 2466
FatI	CATG	9 cut(s)	382, 422, 1101, 1215, 1549, 1832, 1872, 2320, 2481
FauNDI	CATATG	1 cut(s)	2380
FblI	GTMKAC	1 cut(s)	836
FokI	GGATG	7 cut(s)	154, 184, 311, 1118, 2205, 2225, 2354
FriOI	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
FspBI	CTAG	5 cut(s)	509, 768, 798, 2073, 2090
GlaI	GCGC	2 cut(s)	6, 1055
GsaI	CCCAGC	1 cut(s)	333
GsuI	CTGGAG	6 cut(s)	8, 197, 997, 1293, 1413, 1758
HaeII	RGCGCY	2 cut(s)	8, 1057
HaeIII	GGCC	4 cut(s)	51, 239, 988, 1133
HapII	CCGG	3 cut(s)	48, 168, 396
HgaI	GACGC	1 cut(s)	208
HhaI	GCGC	2 cut(s)	7, 1056
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	386, 426, 1105, 1219, 1553, 1836, 1876, 2324, 2485
Hin6I	GCGC	2 cut(s)	5, 1054
HinP1I	GCGC	2 cut(s)	5, 1054
HincII	GTYRAC	2 cut(s)	1516, 1713
HindII	GTYRAC	2 cut(s)	1516, 1713
HinfI	GANTC	4 cut(s)	10, 107, 259, 2304
HpaII	CCGG	3 cut(s)	48, 168, 396
HphI	GGTGA	4 cut(s)	709, 1013, 1036, 1537
Hpy166II	GTNNAC	7 cut(s)	439, 837, 1157, 1516, 1713, 1844, 2081
Hpy188I	TCNGA	8 cut(s)	124, 258, 459, 1194, 1279, 1588, 1653, 2212
Hpy8I	GTNNAC	7 cut(s)	439, 837, 1157, 1516, 1713, 1844, 2081
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	6 cut(s)	131, 443, 1568, 2151, 2378, 2562
HpyCH4III	ACNGT	6 cut(s)	151, 535, 1594, 1693, 1764, 2002
HpyCH4IV	ACGT	7 cut(s)	93, 176, 270, 1400, 1520, 1563, 1956
HpyF3I	CTNAG	5 cut(s)	14, 233, 354, 908, 2209
HpySE526I	ACGT	7 cut(s)	93, 176, 270, 1400, 1520, 1563, 1956
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	386, 426, 1105, 1219, 1553, 1836, 1876, 2324, 2485
HspAI	GCGC	2 cut(s)	5, 1054
KasI	GGCGCC	1 cut(s)	4
KspI	CCGCGG	1 cut(s)	55
Kzo9I	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
LmnI	GCTCC	6 cut(s)	206, 1648, 1739, 1748, 1773, 2176
Lsp1109I	GCAGC	6 cut(s)	169, 391, 440, 1057, 1079, 2422
MaeI	CTAG	5 cut(s)	509, 768, 798, 2073, 2090
MaeII	ACGT	7 cut(s)	93, 176, 270, 1400, 1520, 1563, 1956
MaeIII	GTNAC	6 cut(s)	163, 760, 1480, 1570, 2002, 2485
MalI	GATC	7 cut(s)	282, 528, 750, 772, 1585, 2034, 2186
MboI	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
MboII	GAAGA	7 cut(s)	92, 146, 314, 335, 581, 1403, 2360
MfeI	CAATTG	1 cut(s)	318
MflI	RGATCY	2 cut(s)	770, 1583
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	19
MmeI	TCCRAC	4 cut(s)	1217, 1336, 1561, 2339
MroXI	GAANNNNTTC	2 cut(s)	453, 1090
MseI	TTAA	5 cut(s)	1332, 1403, 1611, 1950, 2049
MslI	CAYNNNNRTG	2 cut(s)	1523, 2027
MspA1I	CMGCKG	3 cut(s)	54, 1070, 1868
MspCI	CTTAAG	1 cut(s)	2048
MspI	CCGG	3 cut(s)	48, 168, 396
MspR9I	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
MunI	CAATTG	1 cut(s)	318
MvaI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
MvnI	CGCG	1 cut(s)	54
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	169
NdeI	CATATG	1 cut(s)	2380
NdeII	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
NheI	GCTAGC	2 cut(s)	797, 2072
NlaIII	CATG	9 cut(s)	386, 426, 1105, 1219, 1553, 1836, 1876, 2324, 2485
NlaIV	GGNNCC	7 cut(s)	6, 20, 1555, 1741, 1769, 1978, 2178
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	1 cut(s)	760
NspI	RCATGY	2 cut(s)	426, 1836
NspV	TTCGAA	1 cut(s)	993
OliI	CACNNNNGTG	1 cut(s)	1523
PaeI	GCATGC	1 cut(s)	1836
PaeR7I	CTCGAG	1 cut(s)	86
PciI	ACATGT	1 cut(s)	422
PdmI	GAANNNNTTC	2 cut(s)	453, 1090
PfeI	GAWTC	3 cut(s)	107, 259, 2304
PflFI	GACNNNGTC	1 cut(s)	554
PleI	GAGTC	1 cut(s)	18
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	1 cut(s)	18
Ppu21I	YACGTR	2 cut(s)	1564, 1957
PpuMI	RGGWCCY	1 cut(s)	19
PscI	ACATGT	1 cut(s)	422
PshBI	ATTAAT	1 cut(s)	1611
PsiI	TTATAA	1 cut(s)	2586
Psp124BI	GAGCTC	2 cut(s)	211, 1942
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
PspFI	CCCAGC	1 cut(s)	329
PspGI	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
PspN4I	GGNNCC	7 cut(s)	6, 20, 1555, 1741, 1769, 1978, 2178
PspPI	GGNCC	3 cut(s)	19, 237, 986
PspPPI	RGGWCCY	1 cut(s)	19
PspXI	VCTCGAGB	1 cut(s)	86
PstI	CTGCAG	1 cut(s)	2068
PstNI	CAGNNNCTG	1 cut(s)	2006
PsuI	RGATCY	2 cut(s)	770, 1583
PsyI	GACNNNGTC	1 cut(s)	554
PvuII	CAGCTG	2 cut(s)	1070, 1868
RsaI	GTAC	5 cut(s)	597, 1233, 1420, 1566, 1955
RsaNI	GTAC	5 cut(s)	596, 1232, 1419, 1565, 1954
RseI	CAYNNNNRTG	2 cut(s)	1523, 2027
SacI	GAGCTC	2 cut(s)	211, 1942
SacII	CCGCGG	1 cut(s)	55
SaqAI	TTAA	5 cut(s)	1332, 1403, 1611, 1950, 2049
Sau3AI	GATC	7 cut(s)	280, 526, 748, 770, 1583, 2032, 2184
Sau96I	GGNCC	3 cut(s)	19, 237, 986
SchI	GAGTC	1 cut(s)	19
ScrFI	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
SfcI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	86
Sfr303I	CCGCGG	1 cut(s)	55
SfuI	TTCGAA	1 cut(s)	993
SgrBI	CCGCGG	1 cut(s)	55
SinI	GGWCC	1 cut(s)	19
SlaI	CTCGAG	1 cut(s)	86
SmiMI	CAYNNNNRTG	2 cut(s)	1523, 2027
SmlI	CTYRAG	2 cut(s)	86, 2048
SmoI	CTYRAG	2 cut(s)	86, 2048
SphI	GCATGC	1 cut(s)	1836
SsiI	CCGC	9 cut(s)	52, 54, 57, 60, 69, 72, 75, 870, 2301
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	5 cut(s)	509, 768, 798, 2073, 2090
SstI	GAGCTC	2 cut(s)	211, 1942
StyD4I	CCNGG	6 cut(s)	167, 469, 900, 1390, 1414, 2227
StyI	CCWWGG	3 cut(s)	774, 1119, 1987
TaaI	ACNGT	6 cut(s)	151, 535, 1594, 1693, 1764, 2002
TaiI	ACGT	7 cut(s)	96, 179, 273, 1403, 1523, 1566, 1959
TaqI	TCGA	6 cut(s)	87, 407, 931, 993, 1620, 2307
TatI	WGTACW	2 cut(s)	595, 1231
TauI	GCSGC	8 cut(s)	54, 57, 60, 63, 72, 75, 78, 872
TfiI	GAWTC	3 cut(s)	107, 259, 2304
Tru1I	TTAA	5 cut(s)	1332, 1403, 1611, 1950, 2049
Tru9I	TTAA	5 cut(s)	1332, 1403, 1611, 1950, 2049
TscAI	CASTG	7 cut(s)	367, 652, 916, 1183, 1769, 2083, 2249
TseFI	GTSAC	1 cut(s)	760
TseI	GCWGC	6 cut(s)	182, 379, 428, 1067, 1070, 2435
Tsp45I	GTSAC	1 cut(s)	760
TspDTI	ATGAA	5 cut(s)	315, 879, 880, 954, 2397
TspGWI	ACGGA	2 cut(s)	232, 384
TspRI	CASTG	7 cut(s)	367, 652, 916, 1183, 1769, 2083, 2249
Tth111I	GACNNNGTC	1 cut(s)	554
Vha464I	CTTAAG	1 cut(s)	2048
VneI	GTGCAC	2 cut(s)	1155, 1842
VpaK11BI	GGWCC	1 cut(s)	19
VspI	ATTAAT	1 cut(s)	1611
XapI	RAATTY	3 cut(s)	118, 1543, 1655
XbaI	TCTAGA	1 cut(s)	767
XceI	RCATGY	2 cut(s)	426, 1836
XcmI	CCANNNNNNNNNTGG	2 cut(s)	221, 1819
XhoI	CTCGAG	1 cut(s)	86
XmiI	GTMKAC	1 cut(s)	836
XmnI	GAANNNNTTC	2 cut(s)	453, 1090
XspI	CTAG	5 cut(s)	509, 768, 798, 2073, 2090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.